Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0004868

Physical Location & Seq

Genomic Coordinates Reverse (-)

34970079 .. 34979391

Gene Structure 9313 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0244200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4542 bp

ATGGACGTTGCTTCCTCCTCTTCTTCTTCTTCTTCTTTGCCCACAGATGTCTTCCTCAGTTTTAGAGGCCAAGACACTCGAGAGAGCTTTGTCTGCCATCTTTACGCAGCTCTGGAACGGCGAGCAGTCAAAGTCTACCTTGATAATGAAGAACTTAAAACAGGCGACGAGCTTTCGGATCTACAGAAAGCCATTGCCGAATCAAAGATTTCGATTGTAGTTTTCTCTGAAAACTACGCGACTTCCACATGGTGCTTGAAAGAACTGGTCAAGATCCTGAAATGCAAGAAGGCGGAGCAGAAGCAGATTGTGATGCCCATTTTCTACCGAGTAGATCCTTCCGACATTCGTAAACAGACGGGAAGTTTTGCCAAAGCTTTTGCCGAGCATGAACGCGACCCCGAAAAGGACAAGGAGGAAGTGCAGAGCTGGAGAACCGCTTTAAAGGAAGTCGCAAGTATAGTTGGCTGCCATTCCAAAAATTTTGGGGATGATGCTCAGCTTATTGATGATATTGTAAAGGAAGTCTTTGATAAAGTGAATCGGATCTCGCCACACATAGAGGATGGCGTGGTTGCAATGGATTCCCATCTGCATAAAGTGGGACGACTATTAAATGCTGCGCCGGATGATGTTGTCTATGTCGGAATCTGGGGAATGGGCGGCTTGGGTAAAACAACCATTGCCAGGGCTGTTTATGACAGAATTTCTCATAAATTTGACCACCGTTGCTTTCTTCCAAATGTGAGGGAACGCTTCACGAGGAACGAAGAAGTACAAATGAAAGTTGAATTTCTATCTGGAATCTTGAACGAAAAAGTCAGCAATTTTGATGGAGCTCTTGTAACTATCTCATATTCCAAAACGCTTGAGATGCACGATTTACTACAAGAAATGGGTAGAAAAATTGAAGAAGGCGGGAAGCGGAGTAGATTTTGGGGTGAAGAAGCTGAGCGTGTGCTTACTGAAAATACGGCTACGGAAAATGCTGGACGCTTGATGATTGACTTGCCGAAAAAGGAAGGCGGATCCTTCAATACTTCAATGCGCATATTCACTTACGGGCAAAGGTATTCTGGAGATGACGGTCAAAATCACTTGACTGGGGAATTCAATTTTCTTTCTCGTGAGTTGAAGGTTCTCGTCTGGCATGGATATCCCCAAAAGTGTTTGCCATCCAACTTTGACCCCAAGAATCTTGTTCAGCTTGACATGCCTTACAGTAACATTGAACAACAACTTTGGGAAGGATCCAAGCCGGCGGAAAACTTGATTAGCATCAATTTGAGTGGCTCTCCATATCTTAAAAGAATCCCTAACTTGACTCTTGCGACAAATCTTAAGGAACTCGACTTTCAAGGTTGTTCAAGTTTAACGGAGGTTGACCCATCCATTTCAGCTCTTAAAAACCTTGTTTTTCTTAATTTCCTTCATTGCGAGGAACTTAGGAGTCTTCCGAGCATTGGTCATATGAAGTCTCTCAAAACTCTTCATCTTGGTTACTGCTTAAAGTTTGAGATGTTTCCAGAGATTTTGGAAGGTATGGAGGCACTATCAACGCTTAAATTAACTCACACTCCAATTAAGGAGTTACCATCGTCAATTGAACGGCTTCGGGGGCTTAAATCATTAGACATGACATTCTGTGGAAGCCTTATCCGTCTTCCAGACAGTATCTGTAATTTAGCAGAACTTAGAGATCTCAGCTTGAGAGACTGCTCAAAACTCTGTAAGTTGCCCGAGAACATTGGGAATTTACAATCTTTGTTGGAACTTCAAGTATACGGAACTGGTTTAGAACAACTGCCAGTCTCTATCTTACGTTTGAAGCTCGGTGAATTATCGTTTTCTGGTTGCGGACAAATGGCGGCTCCCCTTTATTCATGGCCATCATCAATTGAAGATCGTTGTACTGTTGTGGTACTACTTGATTTTAGTGCTTGCAATCTGCTGGAATTATCAGATGCTATTGCTTATTTTTCTTCATTAAAAGTATTAAGGTTGTCCGGAAACAAAAATTTGGAGAGCTTGCCTGCAATCATGAAACGACTTGATTTCTTAGAAGAGCTTATATTGAATGGTTGCAAGAGACTGAGATCAATACCAGAGCTTTCACCAACAATAAGATACATAAATGCACATGATTGCACAGCTTTGGAATCTGTTTCGACACCACAGTCTCCCTACGATATCAGTCGGTGCTTCATATTTTCTAATTGCAGTCAGCTGGTACAGAAAGATGTTTTCAGAGATGTTGTGGAAACTCATCTCCCTCCTCAGCGTAATTGTTTCCGACCCTTTTATGTCTCTTTTCCTGGAAGGAAAATTCCAAAGTGGTTCACCCATCGGTGTAGGGGGTCTTCCGTAACTGCCAAGCTACCACCAAATTGGTTTGACAACAAGTTTTTGGGCTTTACTATATGCGCGGTTACTAATAATAAGCCCCGTGATGATGGACGGATTGTTCGATGTATCTGTACCTTCAAAGGAGATCATTGCAAGTACCGTTTCAGTTTCTATTTGTCTGCCAACTGCCGTGAATCAAATCACATGTTAGTGGGATATATGCCATGGTCTGAATCTGGCATGATTAAGAGAGAGGAAGTCAATGAACGCTGTTACACAGAGGCCAAATTTGAGATACAATGGCACTATAACTCGAATTATGAAATCTACGAATCGAGTGACCCATTCATCAAAAGGTGCGGAGTTCGATTCGTTTTTGCCAACAAGGAGGAAGATTCTGGGGAACCAATGGTAGAAGTTGATAATTTTGAGAGGCGCCTTGAGGGGTGCTCGGTTGAACCCAGTGGTAGTGGCATCACAAGTGACGCTTCCGATGAGGATGAGCAATACCTTAAATTATTATCAAAAGTTTTTAAAGGCGCAAACAGACCACCGAGTTACATCAAAATTGAGGACAGTGATTCATCTGAGGGTAGTGACATCACAGGTGACACCTCCGATGAGGATGAGCAATACCTTACATCATCACAAGTTTTTAAAGGCGCAAACAGAGCACCGAGTTACATCAAAATTGAGGACAATGATTCATTTGGGAAGAGGAAAACTCTGGAAAGTTGGACGCAAGAAGTCAGAGTTACATCGGGTCTGAGCCAAGGGCCTCTGGATGATGGTTTTGTCTGGTTCTGCTGCAGCTTTGGGATTGACGGAAACGAGTATATATGTACACATCGTGGACGTGTCTGTTGGGCCAAAGCTATAGTGCCAGCTCACACTGACCCAACAATCCTTAAAGTTACTTTCGTTGGGAGGCACACTTGTTGGAAATTTCAACCAATGTTGACACACCAGGTAAGAGTTACACCGGCTATGAAGATTGAAGGTCCTTTTGATGGCTTTACATGGAGGAAGTATGACCAAAAGGACATGCCAGGAGCTAAATACCCAAGAGTCTATTACGTATGCACTCCTCAAAATATCGGAGGCTGCATGGCCGTAAAGGAAGTCCAAAGCTCCAATGATAAAAAAATCCTTAAAATTACTTACAGAGGAAGGCACACATGTACACTAGCCTGCACCAACAATGGAAGAAGGGGTGATAGCAAGACCTTCGTGGGCAGTGGTGCATCGAGTATTCATGACCAAGTTGTCCAATCACCAAGCTCACCAGGAGCCAATGGTCATGGAGGAAATGTAATTACAAGTGCTGCTGGGCTTGCTGCTGCACCTGCTGTTGCCACTGAGACTTATGTTTCGGAGAAGAAGGAGAAGAAGAAGCGCAAACAAGAGGAAATAACTGACAGTCCTGTTCCAGTTCCCGCAAAGAAAAGCAAAGGTGAAGAAGTGGCTGAGCTTGAAGTTGAGAAGACAGAAAAGAAGAAGAGGAAGAAGAAGGATAAAGAAAATGGTGTTTTGGATTCTTTAGGTGGGGAGAAATCTGTGAAGGTGAAGAAGCACAAAGATAAAGAAGAAGCTGGTTCACCTCAAACTGAAAAGTCTGAAAAGAAGAAAAAGAAGAAAAACAAAGAGGCTGAGGATCATGGTGCTGATGCTGCCACTGATAATGGCAAGGGTGATGGAGAGGCTGATAAGAGTGAGAAAAAGAAGAAGAAGAAGAAGAAGAAAAAGGATAAATCGGAAGAAGTGGCTGAGCTTGAAGTTGAGAAGACAGAAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAAAGAATAAAGACAATGGTGTTTTGGATGCTTCAGATGGGGAGAAAACTGTGAAGGTGAAGAAGCACAAAGATAAAGAAGAAGCTGGTTCACCTCAAACTGAAAAGTCTGAAAAGAAGAAAAAGAAGAAAAACACAGAGGCTGAGGATCATGGTTCTGATGCTGCCACTGATAATGGCAAGGGTGATGGAGAGGCTGATAAGAGTGAGAAAAAGAAGAAGAAGAAGGAGAAGAAAAAGGATAAATCGGAAGAAGTGGCTGAGCTTGAAGTTGAGAAGACAGAAAAGAAGAAGAAGAGGAAGAAGAAGAAGGATAAAGAAAATGATGGATTCTTCAGATGGGGAGAAATCTGTGAAGGGGAAGAAGCACAAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009506 GO:0009719 GO:0009725 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030054 GO:0032870 GO:0033993 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048545 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

1513

Amino Acids

170.57

Weight (kDa)

8.56

Isoelectric Point (pI)

45.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	16 - 187	3e-54	TIR domain
TIR_2	PF13676	17 - 113	1.7e-15	TIR domain
LRR_14	PF23598	459 - 573	3.6e-10	Leucine-rich repeat region
LRR_13	PF23286	491 - 576	3.9e-08	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	558 - 610	6e-09	Leucine-rich repeat region
LRR_13	PF23286	578 - 726	2.9e-07	Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID	PF20160	771 - 911	8.9e-20	C-JID domain
WRKY	PF03106	1119 - 1176	1.5e-18	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004868)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g14100
malus_domestica	MD01G1056900.v1.1 MD14G1186200.v1.1 MD14G1186400.v1.1 MD14G1186600.v1.1
pyrus_communis	pycom01g08300 pycom01g08310 pycom01g08390 pycom01g08420 pycom01g08480 pycom01g08650 pycom01g08680 pycom01g08710 pycom02g23240
rosa_chinensis	RchiOBHm_Chr1g0324601 RchiOBHm_Chr1g0354681 RchiOBHm_Chr1g0354861
rosa_laevigata	RLG00000028186
rosa_multiflora	Rmu_sc0003940.1_g000003 Rmu_sc0006632.1_g000017
rosa_roxburghii	Rroxscaffold_4G00325320
rosa_rugosa	Rorug01G0244200 Rorug01G0244200 Rorug01G0244300
rosa_samantha	Rh1AG255200 Rh1AG256400 Rh1BG225700 Rh1CG239700
rosa_wichuraiana	Rw1G022330 Rw1G022370 Rw1G022400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3700
AasI	GACNNNNNNGTC	2 cut(s)	2176, 3611
Acc16I	TGCGCA	1 cut(s)	1049
Acc36I	ACCTGC	1 cut(s)	3700
AccB1I	GGYRCC	1 cut(s)	2781
AccI	GTMKAC	2 cut(s)	135, 1782
AccII	CGCG	3 cut(s)	239, 396, 2426
AccIII	TCCGGA	1 cut(s)	2006
AcoI	YGGCCR	2 cut(s)	1886, 3456
AcuI	CTGAAG	2 cut(s)	4182, 4483
AcyI	GRCGYC	1 cut(s)	2782
AdeI	CACNNNGTG	2 cut(s)	252, 3197
AfaI	GTAC	8 cut(s)	777, 1912, 1923, 2232, 2479, 2504, 3190, 3529
AfiI	CCNNNNNNNGG	4 cut(s)	406, 687, 1463, 3356
AflII	CTTAAG	1 cut(s)	1340
AflIII	ACRYGT	3 cut(s)	2550, 3202, 3524
AjiI	CACGTC	1 cut(s)	3203
AjnI	CCWGG	5 cut(s)	686, 2314, 3312, 3394, 3631
AloI	GAACNNNNNNTCC	2 cut(s)	2448, 2480
Alw21I	GWGCWC	3 cut(s)	841, 2798, 3022
Alw26I	GTCTC	7 cut(s)	1482, 1707, 1816, 2083, 2184, 2311, 3701
AlwNI	CAGNNNCTG	2 cut(s)	1677, 4307
Ama87I	CYCGRG	2 cut(s)	78, 1739
Aor13HI	TCCGGA	1 cut(s)	2006
AoxI	GGCC	6 cut(s)	67, 1886, 2628, 3122, 3213, 3456
ArsI	GACNNNNNNTTYG	4 cut(s)	2448, 2480, 3700, 3732
Asp700I	GAANNNNTTC	1 cut(s)	1611
AspLEI	GCGC	7 cut(s)	625, 1050, 2426, 2784, 2888, 3011, 3744
AspS9I	GGNCC	3 cut(s)	3122, 3213, 3347
AvaI	CYCGRG	2 cut(s)	78, 1739
AvaII	GGWCC	1 cut(s)	3347
BalI	TGGCCA	1 cut(s)	1888
BamHI	GGATCC	2 cut(s)	1028, 1250
BanI	GGYRCC	1 cut(s)	2781
BanII	GRGCYC	1 cut(s)	841
BarI	GAAGNNNNNNTAC	2 cut(s)	2344, 2376
BauI	CACGAG	2 cut(s)	760, 1125
BbsI	GAAGAC	7 cut(s)	43, 1445, 1655, 2352, 3836, 4136, 4448
Bbv12I	GWGCWC	3 cut(s)	841, 2798, 3022
BbvCI	CCTCAGC	3 cut(s)	2277, 3996, 4308
BceAI	ACGGC	5 cut(s)	134, 990, 1625, 2520, 3443
BcgI	CGANNNNNNTGC	2 cut(s)	3556, 3590
BciT130I	CCWGG	5 cut(s)	688, 2316, 3314, 3396, 3633
BcoDI	GTCTC	7 cut(s)	1482, 1707, 1816, 2083, 2184, 2311, 3701
BfaI	CTAG	1 cut(s)	3533
BfmI	CTRYAG	3 cut(s)	182, 3154, 3222
BfoI	RGCGCY	1 cut(s)	2785
BfrI	CTTAAG	1 cut(s)	1340
BfuAI	ACCTGC	1 cut(s)	3700
BglII	AGATCT	1 cut(s)	1699
BlpI	GCTNAGC	5 cut(s)	498, 951, 3813, 4113, 4425
Bme1390I	CCNGG	5 cut(s)	688, 2316, 3314, 3396, 3633
Bme18I	GGWCC	1 cut(s)	3347
BmeT110I	CYCGRG	2 cut(s)	78, 1739
BmgBI	CACGTC	1 cut(s)	3203
BmgT120I	GGNCC	3 cut(s)	3122, 3213, 3347
BmiI	GGNNCC	6 cut(s)	1030, 1252, 1872, 2751, 2783, 3637
BmrFI	CCNGG	5 cut(s)	688, 2316, 3314, 3396, 3633
BmrI	ACTGGG	2 cut(s)	1113, 2802
BmuI	ACTGGG	2 cut(s)	1113, 2802
BpiI	GAAGAC	7 cut(s)	43, 1445, 1655, 2352, 3836, 4136, 4448
BplI	GAGNNNNNCTC	6 cut(s)	1279, 1311, 2780, 2812, 3055, 3087
BpmI	CTGGAG	2 cut(s)	451, 1098
Bpu10I	CCTNAGC	3 cut(s)	2277, 3996, 4308
Bpu1102I	GCTNAGC	5 cut(s)	498, 951, 3813, 4113, 4425
BpuEI	CTTGAG	3 cut(s)	890, 1729, 2807
BsaAI	YACGTR	1 cut(s)	3424
BsaBI	GATNNNNATC	2 cut(s)	588, 1277
BsaHI	GRCGYC	1 cut(s)	2782
BsaJI	CCNNGG	3 cut(s)	687, 2570, 3118
BsaWI	WCCGGW	1 cut(s)	2006
BsaXI	ACNNNNNCTCC	6 cut(s)	828, 858, 1561, 1591, 3642, 3672
Bsc4I	CCNNNNNNNGG	4 cut(s)	406, 687, 1463, 3356
Bse118I	RCCGGY	2 cut(s)	1258, 3328
Bse1I	ACTGG	6 cut(s)	270, 1108, 1795, 1808, 2808, 3776
Bse3DI	GCAATG	5 cut(s)	192, 585, 681, 1432, 2494
Bse8I	GATNNNNATC	2 cut(s)	588, 1277
BseAI	TCCGGA	1 cut(s)	2006
BseBI	CCWGG	5 cut(s)	688, 2316, 3314, 3396, 3633
BseDI	CCNNGG	3 cut(s)	687, 2570, 3118
BseGI	GGATG	9 cut(s)	496, 571, 634, 1175, 1388, 2851, 2977, 3136, 4198
BseJI	GATNNNNATC	2 cut(s)	588, 1277
BseLI	CCNNNNNNNGG	4 cut(s)	406, 687, 1463, 3356
BseMI	GCAATG	5 cut(s)	192, 585, 681, 1432, 2494
BseNI	ACTGG	6 cut(s)	270, 1108, 1795, 1808, 2808, 3776
BseRI	GAGGAG	3 cut(s)	7, 2265, 3423
BseYI	CCCAGC	1 cut(s)	3674
BsgI	GTGCAG	3 cut(s)	443, 3523, 3672
Bsh1236I	CGCG	3 cut(s)	239, 396, 2426
BshFI	GGCC	6 cut(s)	69, 1888, 2630, 3124, 3215, 3458
BshNI	GGYRCC	1 cut(s)	2781
BsiHKAI	GWGCWC	3 cut(s)	841, 2798, 3022
BsiHKCI	CYCGRG	2 cut(s)	78, 1739
BsiSI	CCGG	4 cut(s)	626, 1259, 2007, 3329
BslFI	GGGAC	1 cut(s)	618
BslI	CCNNNNNNNGG	4 cut(s)	406, 687, 1463, 3356
BsmAI	GTCTC	7 cut(s)	1482, 1707, 1816, 2083, 2184, 2311, 3701
BsmFI	GGGAC	1 cut(s)	618
BsnI	GGCC	6 cut(s)	69, 1888, 2630, 3124, 3215, 3458
BsoBI	CYCGRG	2 cut(s)	78, 1739
Bsp1286I	GDGCHC	3 cut(s)	841, 2798, 3022
Bsp13I	TCCGGA	1 cut(s)	2006
Bsp1407I	TGTACA	2 cut(s)	3188, 3527
Bsp1720I	GCTNAGC	5 cut(s)	498, 951, 3813, 4113, 4425
Bsp19I	CCATGG	1 cut(s)	2570
BspANI	GGCC	6 cut(s)	69, 1888, 2630, 3124, 3215, 3458
BspEI	TCCGGA	1 cut(s)	2006
BspFNI	CGCG	3 cut(s)	239, 396, 2426
BspHI	TCATGA	2 cut(s)	2040, 3601
BspLI	GGNNCC	6 cut(s)	1030, 1252, 1872, 2751, 2783, 3637
BspMAI	CTGCAG	1 cut(s)	3158
BspMI	ACCTGC	1 cut(s)	3700
BspQI	GCTCTTC	1 cut(s)	2058
BspT107I	GGYRCC	1 cut(s)	2781
BspTI	CTTAAG	1 cut(s)	1340
BsrDI	GCAATG	5 cut(s)	192, 585, 681, 1432, 2494
BsrFI	RCCGGY	2 cut(s)	1258, 3328
BsrGI	TGTACA	2 cut(s)	3188, 3527
BsrI	ACTGG	6 cut(s)	270, 1108, 1795, 1808, 2808, 3776
BssAI	RCCGGY	2 cut(s)	1258, 3328
BssECI	CCNNGG	3 cut(s)	687, 2570, 3118
BssNAI	GTATAC	1 cut(s)	1783
BssNI	GRCGYC	1 cut(s)	2782
BssSI	CACGAG	2 cut(s)	760, 1125
BssT1I	CCWWGG	2 cut(s)	2570, 3118
Bst1107I	GTATAC	1 cut(s)	1783
Bst2BI	CACGAG	2 cut(s)	760, 1125
Bst2UI	CCWGG	5 cut(s)	688, 2316, 3314, 3396, 3633
Bst6I	CTCTTC	6 cut(s)	25, 1494, 2058, 3056, 3839, 4454
BstACI	GRCGYC	1 cut(s)	2782
BstAFI	CTTAAG	1 cut(s)	1340
BstAUI	TGTACA	2 cut(s)	3188, 3527
BstBAI	YACGTR	1 cut(s)	3424
BstC8I	GCNNGC	8 cut(s)	123, 1260, 1942, 2030, 2034, 3231, 3538, 3681
BstDSI	CCRYGG	1 cut(s)	2570
BstF5I	GGATG	9 cut(s)	496, 571, 634, 1175, 1388, 2851, 2977, 3136, 4198
BstFNI	CGCG	3 cut(s)	239, 396, 2426
BstH2I	RGCGCY	1 cut(s)	2785
BstHHI	GCGC	7 cut(s)	625, 1050, 2426, 2784, 2888, 3011, 3744
BstMAI	GTCTC	7 cut(s)	1482, 1707, 1816, 2083, 2184, 2311, 3701
BstMWI	GCNNNNNNNGC	8 cut(s)	93, 874, 1213, 1618, 3017, 3680, 3692, 4016
BstNI	CCWGG	5 cut(s)	688, 2316, 3314, 3396, 3633
BstNSI	RCATGY	4 cut(s)	1216, 2554, 3394, 3528
BstSCI	CCNGG	5 cut(s)	686, 2314, 3312, 3394, 3631
BstSFI	CTRYAG	3 cut(s)	182, 3154, 3222
BstSNI	TACGTA	1 cut(s)	3424
BstUI	CGCG	3 cut(s)	239, 396, 2426
BstV2I	GAAGAC	7 cut(s)	43, 1445, 1655, 2352, 3836, 4136, 4448
BstX2I	RGATCY	7 cut(s)	178, 273, 334, 546, 1028, 1250, 1699
BstXI	CCANNNNNNTGG	1 cut(s)	2388
BstYI	RGATCY	7 cut(s)	178, 273, 334, 546, 1028, 1250, 1699
BstZ17I	GTATAC	1 cut(s)	1783
BsuRI	GGCC	6 cut(s)	69, 1888, 2630, 3124, 3215, 3458
BtgI	CCRYGG	1 cut(s)	2570
BtrI	CACGTC	1 cut(s)	3203
BtsCI	GGATG	9 cut(s)	496, 571, 634, 1175, 1388, 2851, 2977, 3136, 4198
BtsI	GCAGTG	1 cut(s)	3589
BtsIMutI	CAGTG	7 cut(s)	2815, 2929, 3237, 3589, 3702, 4020, 4332
BveI	ACCTGC	1 cut(s)	3700
Cac8I	GCNNGC	8 cut(s)	123, 1260, 1942, 2030, 2034, 3231, 3538, 3681
CaiI	CAGNNNCTG	2 cut(s)	1677, 4307
CciI	TCATGA	2 cut(s)	2040, 3601
CfoI	GCGC	7 cut(s)	625, 1050, 2426, 2784, 2888, 3011, 3744
Cfr10I	RCCGGY	2 cut(s)	1258, 3328
Cfr13I	GGNCC	3 cut(s)	3122, 3213, 3347
CseI	GACGC	3 cut(s)	1002, 2840, 3094
CsiI	ACCWGGT	1 cut(s)	3312
Csp6I	GTAC	8 cut(s)	776, 1911, 1922, 2231, 2478, 2503, 3189, 3528
CspCI	CAANNNNNGTGG	2 cut(s)	1270, 1305
CviQI	GTAC	8 cut(s)	776, 1911, 1922, 2231, 2478, 2503, 3189, 3528
DinI	GGCGCC	1 cut(s)	2783
DraI	TTTAAA	3 cut(s)	444, 2881, 3004
DraIII	CACNNNGTG	2 cut(s)	252, 3197
DrdI	GACNNNNNNGTC	2 cut(s)	2176, 3611
DseDI	GACNNNNNNGTC	2 cut(s)	2176, 3611
EaeI	YGGCCR	2 cut(s)	1886, 3456
Eam1104I	CTCTTC	6 cut(s)	25, 1494, 2058, 3056, 3839, 4454
EarI	CTCTTC	6 cut(s)	25, 1494, 2058, 3056, 3839, 4454
EciI	GGCGGA	3 cut(s)	308, 1041, 1277
Ecl136II	GAGCTC	1 cut(s)	839
Eco105I	TACGTA	1 cut(s)	3424
Eco130I	CCWWGG	2 cut(s)	2570, 3118
Eco24I	GRGCYC	1 cut(s)	841
Eco32I	GATATC	2 cut(s)	1157, 2191
Eco47I	GGWCC	1 cut(s)	3347
Eco53kI	GAGCTC	1 cut(s)	839
Eco57I	CTGAAG	2 cut(s)	4182, 4483
Eco88I	CYCGRG	2 cut(s)	78, 1739
EcoICRI	GAGCTC	1 cut(s)	839
EcoO109I	RGGNCCY	2 cut(s)	3122, 3347
EcoRI	GAATTC	1 cut(s)	1109
EcoRII	CCWGG	5 cut(s)	686, 2314, 3312, 3394, 3631
EcoRV	GATATC	2 cut(s)	1157, 2191
EcoT14I	CCWWGG	2 cut(s)	2570, 3118
EcoT38I	GRGCYC	1 cut(s)	841
EgeI	GGCGCC	1 cut(s)	2783
EheI	GGCGCC	1 cut(s)	2783
ErhI	CCWWGG	2 cut(s)	2570, 3118
FalI	AAGNNNNNCTT	6 cut(s)	123, 155, 512, 544, 2818, 2850
FaqI	GGGAC	1 cut(s)	618
FauI	CCCGC	2 cut(s)	911, 3790
FauNDI	CATATG	1 cut(s)	1470
FblI	GTMKAC	2 cut(s)	135, 1782
FokI	GGATG	9 cut(s)	503, 578, 641, 1162, 1375, 2858, 2984, 3143, 4205
FriOI	GRGCYC	1 cut(s)	841
FspAI	RTGCGCAY	1 cut(s)	1049
FspBI	CTAG	1 cut(s)	3533
FspI	TGCGCA	1 cut(s)	1049
GlaI	GCGC	7 cut(s)	624, 1049, 2425, 2783, 2887, 3010, 3743
GsaI	CCCAGC	1 cut(s)	3678
GsuI	CTGGAG	2 cut(s)	451, 1098
HaeII	RGCGCY	1 cut(s)	2785
HaeIII	GGCC	6 cut(s)	69, 1888, 2630, 3124, 3215, 3458
HapII	CCGG	4 cut(s)	626, 1259, 2007, 3329
HgaI	GACGC	3 cut(s)	1002, 2840, 3094
HhaI	GCGC	7 cut(s)	625, 1050, 2426, 2784, 2888, 3011, 3744
Hin1I	GRCGYC	1 cut(s)	2782
Hin6I	GCGC	7 cut(s)	623, 1048, 2424, 2782, 2886, 3009, 3742
HinP1I	GCGC	7 cut(s)	623, 1048, 2424, 2782, 2886, 3009, 3742
HincII	GTYRAC	2 cut(s)	1384, 3306
HindII	GTYRAC	2 cut(s)	1384, 3306
HindIII	AAGCTT	1 cut(s)	375
HpaII	CCGG	4 cut(s)	626, 1259, 2007, 3329
Hpy99I	CGWCG	1 cut(s)	170
HpyCH4IV	ACGT	4 cut(s)	6, 1822, 3202, 3423
HpyF10VI	GCNNNNNNNGC	8 cut(s)	93, 874, 1213, 1618, 3017, 3680, 3692, 4016
HpySE526I	ACGT	4 cut(s)	6, 1822, 3202, 3423
Hsp92I	GRCGYC	1 cut(s)	2782
HspAI	GCGC	7 cut(s)	623, 1048, 2424, 2782, 2886, 3009, 3742
KasI	GGCGCC	1 cut(s)	2781
Kpn2I	TCCGGA	1 cut(s)	2006
KroI	GCCGGC	1 cut(s)	1258
KroNI	GCCGGC	1 cut(s)	1260
LguI	GCTCTTC	1 cut(s)	2058
LmnI	GCTCC	6 cut(s)	295, 836, 1876, 3398, 3482, 3635
MabI	ACCWGGT	1 cut(s)	3312
MaeI	CTAG	1 cut(s)	3533
MaeII	ACGT	4 cut(s)	6, 1822, 3202, 3423
MfeI	CAATTG	2 cut(s)	1602, 1896
MflI	RGATCY	7 cut(s)	178, 273, 334, 546, 1028, 1250, 1699
MhlI	GDGCHC	3 cut(s)	841, 2798, 3022
MlsI	TGGCCA	1 cut(s)	1888
MluNI	TGGCCA	1 cut(s)	1888
Mly113I	GGCGCC	1 cut(s)	2782
MlyI	GAGTC	3 cut(s)	1318, 1459, 3423
MmeI	TCCRAC	7 cut(s)	366, 625, 1203, 1749, 2317, 3062, 3266
Mox20I	TGGCCA	1 cut(s)	1888
MroI	TCCGGA	1 cut(s)	2006
MroNI	GCCGGC	1 cut(s)	1258
MroXI	GAANNNNTTC	1 cut(s)	1611
MscI	TGGCCA	1 cut(s)	1888
MslI	CAYNNNNRTG	1 cut(s)	2555
Msp20I	TGGCCA	1 cut(s)	1888
MspA1I	CMGCKG	1 cut(s)	2227
MspCI	CTTAAG	1 cut(s)	1340
MspI	CCGG	4 cut(s)	626, 1259, 2007, 3329
MspR9I	CCNGG	5 cut(s)	688, 2316, 3314, 3396, 3633
MunI	CAATTG	2 cut(s)	1602, 1896
MvaI	CCWGG	5 cut(s)	688, 2316, 3314, 3396, 3633
MvnI	CGCG	3 cut(s)	239, 396, 2426
MwoI	GCNNNNNNNGC	8 cut(s)	93, 874, 1213, 1618, 3017, 3680, 3692, 4016
NaeI	GCCGGC	1 cut(s)	1260
NarI	GGCGCC	1 cut(s)	2782
NcoI	CCATGG	1 cut(s)	2570
NdeI	CATATG	1 cut(s)	1470
NgoMIV	GCCGGC	1 cut(s)	1258
NlaIV	GGNNCC	6 cut(s)	1030, 1252, 1872, 2751, 2783, 3637
NmeAIII	GCCGAG	1 cut(s)	409
NmuCI	GTSAC	4 cut(s)	2684, 2828, 2942, 2954
NsbI	TGCGCA	1 cut(s)	1049
NspI	RCATGY	4 cut(s)	1216, 2554, 3394, 3528
PaeR7I	CTCGAG	1 cut(s)	78
PagI	TCATGA	2 cut(s)	2040, 3601
PaqCI	CACCTGC	1 cut(s)	3700
PciI	ACATGT	2 cut(s)	2550, 3524
PciSI	GCTCTTC	1 cut(s)	2058
PcsI	WCGNNNNNNNCGW	1 cut(s)	2464
PdiI	GCCGGC	1 cut(s)	1260
PdmI	GAANNNNTTC	1 cut(s)	1611
PfoI	TCCNGGA	1 cut(s)	2314
PleI	GAGTC	3 cut(s)	1318, 1458, 3422
PluTI	GGCGCC	1 cut(s)	2785
PpsI	GAGTC	3 cut(s)	1318, 1458, 3422
Ppu21I	YACGTR	1 cut(s)	3424
PpuMI	RGGWCCY	1 cut(s)	3347
PscI	ACATGT	2 cut(s)	2550, 3524
Psp124BI	GAGCTC	1 cut(s)	841
Psp5II	RGGWCCY	1 cut(s)	3347
Psp6I	CCWGG	5 cut(s)	686, 2314, 3312, 3394, 3631
PspFI	CCCAGC	1 cut(s)	3674
PspGI	CCWGG	5 cut(s)	686, 2314, 3312, 3394, 3631
PspN4I	GGNNCC	6 cut(s)	1030, 1252, 1872, 2751, 2783, 3637
PspPI	GGNCC	3 cut(s)	3122, 3213, 3347
PspPPI	RGGWCCY	1 cut(s)	3347
PstI	CTGCAG	1 cut(s)	3158
PstNI	CAGNNNCTG	2 cut(s)	1677, 4307
PsuI	RGATCY	7 cut(s)	178, 273, 334, 546, 1028, 1250, 1699
PvuII	CAGCTG	1 cut(s)	2227
RsaI	GTAC	8 cut(s)	777, 1912, 1923, 2232, 2479, 2504, 3190, 3529
RsaNI	GTAC	8 cut(s)	776, 1911, 1922, 2231, 2478, 2503, 3189, 3528
RseI	CAYNNNNRTG	1 cut(s)	2555
SacI	GAGCTC	1 cut(s)	841
SapI	GCTCTTC	1 cut(s)	2058
Sau96I	GGNCC	3 cut(s)	3122, 3213, 3347
SchI	GAGTC	3 cut(s)	1318, 1459, 3423
ScrFI	CCNGG	5 cut(s)	688, 2316, 3314, 3396, 3633
SduI	GDGCHC	3 cut(s)	841, 2798, 3022
SexAI	ACCWGGT	1 cut(s)	3312
SfcI	CTRYAG	3 cut(s)	182, 3154, 3222
SfoI	GGCGCC	1 cut(s)	2783
Sfr274I	CTCGAG	1 cut(s)	78
SinI	GGWCC	1 cut(s)	3347
SlaI	CTCGAG	1 cut(s)	78
SmiMI	CAYNNNNRTG	1 cut(s)	2555
SmlI	CTYRAG	5 cut(s)	78, 869, 1340, 1708, 2786
SmoI	CTYRAG	5 cut(s)	78, 869, 1340, 1708, 2786
SnaBI	TACGTA	1 cut(s)	3424
SspDI	GGCGCC	1 cut(s)	2781
SspMI	CTAG	1 cut(s)	3533
SstI	GAGCTC	1 cut(s)	841
StyD4I	CCNGG	5 cut(s)	686, 2314, 3312, 3394, 3631
StyI	CCWWGG	2 cut(s)	2570, 3118
TaiI	ACGT	4 cut(s)	9, 1825, 3205, 3426
TaqI	TCGA	9 cut(s)	79, 212, 1350, 2168, 2467, 2660, 2681, 2713, 3593
TatI	WGTACW	4 cut(s)	775, 1910, 3188, 3527
TauI	GCSGC	2 cut(s)	666, 1871
TscAI	CASTG	7 cut(s)	2815, 2929, 3244, 3589, 3709, 4027, 4339
TseFI	GTSAC	4 cut(s)	2684, 2828, 2942, 2954
Tsp45I	GTSAC	4 cut(s)	2684, 2828, 2942, 2954
TspGWI	ACGGA	7 cut(s)	995, 1391, 1649, 1800, 2353, 2473, 3186
TspRI	CASTG	7 cut(s)	2815, 2929, 3244, 3589, 3709, 4027, 4339
Vha464I	CTTAAG	1 cut(s)	1340
VpaK11BI	GGWCC	1 cut(s)	3347
XceI	RCATGY	4 cut(s)	1216, 2554, 3394, 3528
XhoI	CTCGAG	1 cut(s)	78
XmiI	GTMKAC	2 cut(s)	135, 1782
XmnI	GAANNNNTTC	1 cut(s)	1611
XspI	CTAG	1 cut(s)	3533

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.