Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0002197

Physical Location & Seq

Genomic Coordinates Forward (+)

33833301 .. 33841920

Gene Structure 8620 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0303000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6867 bp

ATGGATGTGCCTTCGTCATCTTCTTCCAAAACCCAAACAGTTACTTCGTCATCATCTAGTTCACCCTCTAACTGGGAACATGATGTCTTCCTCAGTTTTAGGGGCGAGGACACCCGCAATAGTTTTACAGACCATTTACACTATGCTATGAACCAGAGAGGAATCGACACATTTCGAGATACCGAAAAGCTTCAGAGGGGGAAATCCATTTCACCAGAACTTCTGAAAGCAATAGAAGAGTCCAAGTTTGCAGTTACCGTTCTTTCAACCAACTATGCTACTTCAACTTGGTGTCTGGATGAACTTGCGCATATTCTTGAATGCAAGAAACTGAGGGGACTACAAGTTCTTCCGGTTTTTTATCATGTAGAACCATCTGAGATAAGGAAGCAAACTGGAAACTATGGCAAGGCTTTTGCTAAGCATGAGATACATTTCAAGGACAATATGAGAAAGGTGGACAAGTGGAGGAAAGCTTTAGAGGATATAGCTGGTCTCTCCGGATGGCATGTGACAGAGGATAGGAGAGAATCAGAAGTTATTCAAGAAGTAGTTAATCAGATTCTAAATGTACTGAACAAAATGTTATCCGTTCCTGAAAGAGAGTTAATCGGAATGGATGCCCGTATAATAGAAATAGAGTCTCGCTTGGATCTAGAGTCAAATGATGTTCTTACCGTAGGGATTTGGGGGATGGGGGGAATTGGTAAGACAACTCTTGCTAAGGAAGTATTCAAAAAGATCTGTAACCAATTTCATCCTAGCGGCTTCGTTTCCCAAGTTAGATTGCAATCTGAAGTTGAGTTGCATCGACATCTCTGTGAATCCTTTTTGGGGGATGGCAATATAAATATCGACACCAGTGAAAAAGGGATCAAGTTATTGAAGAAGGCACTGTATAAGAAAAAGGTGCTTATTGTTCTGGATGATGTTGATGAATTCAAACAAATAGAATGTCTAGCACCTGGAGGCCCGCTTGGAGAGAATATTTGGGGTGGAGGGAGTCGACTGATTATAACAACTAGAGATAGGAGCCCATTGAGAAACTTTAATGTACAAGAAAATAAAATATATGAGGTTGAAAAACTGAGAGATGAGGAAGCTTTTCAGCTGTTATGTCAGAAAGCCTTCAAGAAAGACAATCCCCCAGAAGAGTTTGTAGCGTTGTCCAAGAGTTTTCTGCAATATGCTAGTGGCCTTCCTTTAGCTCATGAAGTTCTAGGGTCATACTTGTCTAGACTAAAGGTAGATGAATGGTCAGAGATATTGCATAGACTAGACGATGATCAAGATAAAGACATTTTCAGTGTGCTTCAAATAAGCTTCGATGGATTACAGGATACAGATAGGAAAATATTTTTGGACATTGCATGTTTCTTCAATGGCGAGGATCATGTCCGTGTAAAGAATATATTGAAAGGTTGTGGCTTTTCTTCCAGAATAGGTATAAGCAACCTCATTGACAAGTCTCTGATTAAAATTGAAAGAAATAAACTGTGGATGCATGATTTACTACGGTGCTTGGGTTGGCATATTGTTCGTGGAGAATCTTCTTTTCCAGGTCAACGTAGCAGGTTGTGGCTCAATGACAATGTACACAAGTACGAAGGCAGAGGGTCATGGCGTTTTGAGGATGCTCGTAGCGTGCTCATGGACAATACGGGGACAACTACTGTTGAAGGCTTATTCTTAAGCTTGCCTGAAAAAGAAGAAATGCCATTGGAAGATGACCCGTTCTTAACTATGGGCAACCTACGATTGCTGAAGATTTGTAATGTAAATTTTCTGGATGTGCACTTCAGATATGTCTCTAAGAATTTACGACTTTTGGAATGGCACGAATGCCCTCTAGTATCTCTGCCATCTAGTTTTAAATCGGACAAGTTGGTTGAATTAAAGATGCCTAACAGCCGCATTGAACAACTATGGAATGAAACGCTTTCTCTGAAAATGCTTATACTCATGGATCTTTCTGACTGCAAGTATTTAACCAAGACCCTGGACTTCAGTAAGATCCCAAAGCTTGAGAAATTGATCCTTAAAGGTTGTATAGAGTTATTGGAGGTTCACCCTACTATTGGGGATCTCCAACATTTGGTTTTATTGAACTTGAAAGGTTGTGAAAGTCTAGAGAACCTTCCTCAATCCATCAGGTTGAGATCTCTTCGAACTTTTATTCTTTCGGGATGTTCAAAACTCAGACACTTTCCAGAGATTGTGGGGAACATGGATACTTTATCAGAACTTTATTTAGATGGCACGGCTATACGGGAGCTGCCTGTATCAATCCAGCATTTGGAAGGCCTCATTTTACTTAATCTAAGTGGGTGCAGGAACCTTCTCAGTCTTCCAAGCATCCTTTCTAGTAGTTTGACATCACTGAAATTTCTCTATCTGTCACTATGCTCACGTATGGACAGACTGCCGGATAACATAGGCTGCTTGGAACACTTGGAGGAGCTTGATGCGTGTAATATTGCTATAAGAAAAGTGCCCGATTCCATTTTTCTCCTCAAGAATCTTAAATTATTGTGTTTCCATGGCTGTGCTAGATCCACAGGCTTAGAGTTGCCAAGCAAGTTCTCAGGTTTAAGATCTTTGACAACACTAAACCTAGGTGGATGTAATCTCGCAGAAGGAGCAATCCCCAATGACATTGGTGATTTATTCTCACTGCAGAGTCTGGATTTAAGTGAGAACAACTTTTTCACCATACCTGAAAGCATCTCTCAGCTTTCTGAGCTTACAGAAATTTCTCTGTTTAAGTGTAGCAAACTATGGACGTTACCAAAAGATCTTCCGTCAAGTCTAAGAAATTTAAATGTACGTGGTTGTCCTATGCTGACAAGTTCTTCATCTAGTTGGAGGAGATATCCGCCTCAAAAGGGTTTGAGTATCATAAACTGTCGAAAACCAGAGGAGGATGAAATCTTTCCCGAACTCTATAAGTTTCGTTTGGGAAATCTGGAAGAACTCGATCTTTCCAACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCAAATCTTAAGAAATTGAACCTTGAAGGTTGTGAAAAATTATCAGAGGTTCACCCAACCATTGGGTGTCTCCAACATTTGGTGTTTCTGAATTTGAAAGGATGTGTAAATCTAGAGAGCCTTCCCAGTTCTATCAGCTTGAAGTCTCTAAACATGTTTGTTCTTTCGGGATGTTCAAAGCTGAAAGAGTTTCCAAACATTAAGGGAGAAATGAACAATTTGTCACAACTGCATTTAGATGGGACGGCGTTAAGGCATCTGCGAATTCCGATGATGCATTTGAAAGGCCCAATTTTGATAAATCTGTCAGGCTGCAGGAACCTTTTGACTGTTCCAATCCTATTCAGTCTAAAATCTCTCAATCTATCACGGTGCTCAACTATATTCAAATTGCCACCGAGCCTGGCATACATGGAACATTTGGAGGAGCTTGATGCCTCTGAAACTGCTATAACAGGATTACCCCAGTCCATTTTACTCTTGAAGAAACTTAAAGTGTTATCATTCTGTGGATGCAAAGGTTTGCAATTGCCTAAATGGTTCTCGGATTTAAGCTCTTTGACATCATTAAATCTACGGAGGTGTAGTCTAGCAGAAGAAGTCCTTGATAGCCTCTACAGCTTATCTGCTCTGCAGATTTTGGATTTGAGTGAAAACAATCTTTCGATCATACCTAGTGGAATTGGTCGCTTATCCTCTTTGAAGCTATTGAATTTGAGTGAGAACAATTTTGACAATATACCAAATGAAATCGGTCACTTATCCTCTTTGCAGGTCTTAGATTTGAGTGAAAACAGCTTCATGAGTATACCTGATGAGAGCATCTCTCATCTTTCTGAGCTGACAGAACTACGCTTGTTTAGATGTAGTAAGCTACAGTCCTTGCCGAATAATCTTCCCTTCAATCTAAAACATGTCCATGCACAAGAATGTGCTATGTTGAAGAATAACGCAGATACTTTGACAATATGGGCATCTGGAAAGGGGTTCTGTTTCATAAATTGCAGACAATCTGACCAGGATGATGGTCAGCCAAATCACCTCCCAGTTCCAGTTCCCAAAGACCGTATTGAACTACTCTTTCCTATATACATTGAGGACCGAGTTTATGGCAAAAAACCATTTGAAATTCGCTTTCCACATTCGTGGAGTACAGGACTGAGTGCCGGAATTCCAAATTCGTGGAGTCGTTGGAGAAATGGCCCTTCCGTGACAATCCCACTCTCTGATGGTAACAGTACGTGGATGGGACTTGCTCTTTTTGTTGTTTTTGATATCCTTGAGCAAGACATTTTCAACAAGAGCTGGGAATCGGAGGAGACAGTCTGTGATTTTCACACCGATGTTGGGCATGATACTTCCCTAGTTTTTCAAAACTTCATAGACTTCAGGACTGGGTCATATGGTCTTAGTTGCTATGAACCACGTGGTGGGCAATTTAGTGGGTTGTTCGATAAACCAAGTTCACGACTTCGAGCTTCAGTTTCAACAAAGAGACCAAATTTAAAAGTTAGAGGTTGTGGGATACATCTAATATCAGAGGAATATGCTGCAGAGTTTGTTAAAAGTATAGCAACTCAGACTACTAATTCAACTCAGAGTCATCTTGATTCCAATTTTGATCGACATTGCGAGGACATATTAGATGAGGAAACAACTGGTGCCCTGATAGAACAGGGATCTACTAATTCAACTTTCAATGAAGATAGCTGCAGCAAAGTTAACTCTAAGATCAAACTCAGAGGAGAGCTGTCGATACTCTATGAGGGAAGCAAGGGACGTCAGAAGAGTTTCCACTTTTGCTTTCCTGCTTCAGTAATTTCAACCCTGCCTTGGTTCTTCCATCACCATGCAGGGGATGTAACATTGTGTTACATCACTAAAAACTTACTTGATGATCAGAGATGGGTGGGATTAGAACTATATGTTCAATTTTCTCGGTGTACATCTACTTCGACATCTGGCAATAGTAGTTTTTTCTTTTATGTTGATTTATGCTCTCATGATCATGGAAGTATGGTAATGCACGGATCCCTCAAGATAAAGAGTTGTGTTGGGACCTCAGATCAACTTGTTGTATTACATGTACCACGTGTTCATTTTCAACAACAGTTGAATCAATGTCAGGGTATCAGCGCATTGTTCAGAACCATTAATGCAGAAATGGAGGTCCAAGTGTGTGGAAGCCGTTTAGCATTTGAGCATGATTTGGAAGACTTGACCCATTCATTAACTGCTGCTGCCAGCACTTTGGGGCAACATGTCCTCACTCAACTATGTTCCCAGGCCCAACCTGTTGATCGACGCAATGCAGAGGAAGCAGAAATGCCCATCAATTGCTGTTCCTGGTTTAGAAGAAGTACTGCACTTGTACTACCAGAACAAGCTCCTTCTTCTTCAATTGGGCGGCTGCGAAATCATAGATGCTATCTCCAGCAACAGCAAGGTTTTGGGGAGTCTGACCTTACCCAATTGGTCCACTCCAGATCAGTAACATTACTCCACTCGAAAAGCCTTTTGGAAAACAGGGATAGAAACCATGAAAACGTTGCCGAAGAAGATAAATCGTTGGTGTTGGCTCGTCATCATGTACATATAATGGCTGAGACTCAACTTCAGGGAAGATATAGTTTACCACGGTGGAAGAGATGCCTGAAGTTGTTGCTTCGACAGTCCAAGGTGGCTACTCTCAGTCTTTGTGGACACGCAATTTCAGCTTTCAAGAATTTTGATCCTTTCTCCCCATACAATATTATTTGTTTCTCTGACAAGGAAATTCCAGTGTGGTTCAAACATGAGATGTCATATCAGATGTCTTCTAGGTCTAGGGTGGGAATCAAACTACCTCCAAGATTGCACGAGGATGAGAACTGGAAAGGACTTGCTATATGTGTTGCGTTTGAAGTTCATGACCAGCGTCCAACTACCTCCCCGGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAACTATTGCTTGAATCATATCCCTATGTGTTGCATCAATGAAGAGAAACTCAAGTCGCTGCATCTTGGTAGATTCATTTGGCTAACCTACATTCCCCGTATTTTGCTAACGGAGTTCAGTGTGATTAGTGATGTAGAGGCCAGAATTTATGTTAGTTGCAGAGGCTTGACAGTAGAGAAGAGTGGTATACGTCTCTTGTACAGGCAAGAAGAGGGGGAGTTTGAGAACACAATAACCGAGTGCTGGACATCTTTCTTTGATAATCTGTCTTTCATCCGTCAACTAGTAGAAGCAGATGATCAAAATATTCAACCATGGATAAGACATGAACTTCCGATGCTTGAAGGTCATATCAAGGTCTTTGAACCGGATTTAATATATAATGCAATCCCCCCGTCTAATGAAATTCCGGAGTGGTTCGGGCACCGCATTGAGGACCCCTGGGATGCCAGCTGTGGCTGGCAATTCCAGTTACCACCACCTTTGAGTGACACAAATTGGATAGGATTAGCTCTCTTTGTATCATATCGTATTAGCAGAAATTATTTGCAGGAAAGTGTTTTGTACCCCTTTATTATATCATTGAAAACTGAGAAGAATGGTTTGTCCTCTCTCCATCGGTATCAAATGAGCAATGAAGAATTTGAGTTCCTAAAGCGCTGTTGTACTGTGCATCGTGAATTTTTCTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAGCTAAATGATGAGTCTGTCCTCATAGTTTTATCTGGTCTGAACTGCTGGACGCCGGACATGGTCTATCTTCGTTTTGTGTATGCGGATGAGGTGGAAGAGTTTAAGCAGCTCTGTTTCAATCTTCATCGACCCCCTGCCCAACAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0001101 GO:0003674 GO:0003700 GO:0006355 GO:0006357 GO:0006950 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009891 GO:0009893 GO:0010029 GO:0010033 GO:0010035 GO:0010187 GO:0010468 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0019216 GO:0019217 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0033993 GO:0040008 GO:0042221 GO:0042304 GO:0045723 GO:0045834 GO:0045923 GO:0046889 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0060255 GO:0062012 GO:0062013 GO:0065007 GO:0080090 GO:0097305 GO:0140110 GO:1900140 GO:1901700 GO:1901957 GO:1901959 GO:1903506 GO:2000026 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AP2

Protein Analysis

2288

Amino Acids

260.45

Weight (kDa)

6.28

Isoelectric Point (pI)

48.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	27 - 197	3.8e-54	TIR domain
TIR_2	PF13676	29 - 125	1.6e-14	TIR domain
NB-ARC	PF00931	213 - 380	1.1e-21	NB-ARC domain
NACHT	PF05729	226 - 374	4.9e-06	NACHT domain
WHD_ROQ1	PF23282	448 - 517	1.2e-18	Disease resistance protein Roq1-like, winged-helix domain
LRR_14	PF23598	690 - 797	2.7e-06	Leucine-rich repeat region
LRR_13	PF23286	719 - 787	1.5e-08	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	772 - 872	1.9e-10	Leucine-rich repeat region
LRR_14	PF23598	977 - 1088	7.2e-06	Leucine-rich repeat region
LRR_4	PF12799	988 - 1025	2.2e-06	Leucine Rich repeats (2 copies)
LRR_14	PF23598	1111 - 1226	3.9e-11	Leucine-rich repeat region
LRR_8	PF13855	1195 - 1253	9.8e-06	Leucine rich repeat
LRR_14	PF23598	1209 - 1306	5.4e-12	Leucine-rich repeat region
LRR_8	PF13855	1242 - 1298	2.5e-07	Leucine rich repeat
C-JID	PF20160	1931 - 2068	6.8e-07	C-JID domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002197)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16060 AT1G16060 AT1G16060 AT1G16060 AT1G16060 AT1G16060 AT1G16060 AT1G79700 AT1G79700 AT1G79700 AT1G79700
fragaria_vesca	FvH4_4g08800 FvH4_6g28200 FvH4_6g28200 FvH4_6g28200
malus_domestica	MD09G1228500.v1.1 MD16G1258700.v1.1 MD17G1226700.v1.1
prunus_persica	Prupe.1G101600_v2.0.a1 Prupe.3G104100_v2.0.a1
pyrus_communis	pycom09g14740 pycom17g23010
rosa_chinensis	RchiOBHm_Chr2g0132251 RchiOBHm_Chr4g0405371
rosa_laevigata	RLG00000008847 RLG00000019240
rosa_multiflora	Rmu_sc0006313.1_g000002 Rmu_sc0011487.1_g000002
rosa_roxburghii	Rroxscaffold_2G00112250 Rroxscaffold_5G00349830
rosa_rugosa	Rorug02G0303000 Rorug02G0303000 Rorug04G0057800
rosa_samantha	Rh2AG356200 Rh2BG362600 Rh2CG339700 Rh2DG379500 Rh4AG131300 Rh4BG125900 Rh4CG138400 Rh4DG125400
rosa_wichuraiana	Rw2G028940 Rw4G010640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1016
AatII	GACGTC	1 cut(s)	4810
Acc16I	TGCGCA	1 cut(s)	309
Acc36I	ACCTGC	2 cut(s)	1563, 3790
AccB1I	GGYRCC	2 cut(s)	4688, 6417
AccB7I	CCANNNNNTGG	5 cut(s)	2095, 2296, 3089, 3106, 4457
AccI	GTMKAC	3 cut(s)	1006, 3835, 6181
AccIII	TCCGGA	2 cut(s)	500, 6403
AciI	CCGC	8 cut(s)	115, 765, 974, 1912, 2876, 5434, 6421, 6803
AclI	AACGTT	1 cut(s)	5574
AcvI	CACGTG	2 cut(s)	4454, 5120
AcyI	GRCGYC	2 cut(s)	4807, 6770
AdeI	CACNNNGTG	1 cut(s)	4457
AfeI	AGCGCT	1 cut(s)	6653
AflII	CTTAAG	2 cut(s)	1690, 3035
AflIII	ACRYGT	5 cut(s)	3180, 3940, 5110, 5119, 5287
AhlI	ACTAGT	1 cut(s)	6277
AjnI	CCWGG	8 cut(s)	964, 1558, 1998, 3429, 4044, 5310, 5372, 6434
AjuI	GAANNNNNNNTTGG	6 cut(s)	1343, 1375, 2344, 2376, 5528, 5560
AleI	CACNNNNGTG	1 cut(s)	4171
AloI	GAACNNNNNNTCC	2 cut(s)	330, 362
Alw21I	GWGCWC	3 cut(s)	1650, 1797, 3404
Alw44I	GTGCAC	1 cut(s)	1793
AlwNI	CAGNNNCTG	1 cut(s)	2683
Aor13HI	TCCGGA	2 cut(s)	500, 6403
Aor51HI	AGCGCT	1 cut(s)	6653
AoxI	GGCC	7 cut(s)	970, 1195, 2302, 3313, 4228, 5313, 6132
ApaLI	GTGCAC	1 cut(s)	1793
ArsI	GACNNNNNNTTYG	2 cut(s)	940, 972
AseI	ATTAAT	1 cut(s)	5181
Asp700I	GAANNNNTTC	6 cut(s)	189, 540, 1104, 1127, 2931, 4817
AspA2I	CCTAGG	1 cut(s)	2614
AspLEI	GCGC	3 cut(s)	310, 5165, 6654
AspS9I	GGNCC	9 cut(s)	971, 3314, 4126, 4229, 5085, 5197, 5314, 5503, 6430
AsuC2I	CCSGG	1 cut(s)	5960
AsuHPI	GGTGA	8 cut(s)	54, 204, 2060, 2672, 2701, 3071, 4058, 4865
AsuII	TTCGAA	1 cut(s)	2167
AvaII	GGWCC	5 cut(s)	4126, 5085, 5197, 5503, 6430
AvrII	CCTAGG	1 cut(s)	2614
BaeGI	GKGCMC	4 cut(s)	1797, 2496, 4693, 6420
BamHI	GGATCC	1 cut(s)	5057
BanI	GGYRCC	2 cut(s)	4688, 6417
BanII	GRGCYC	1 cut(s)	1037
BarI	GAAGNNNNNNTAC	2 cut(s)	4963, 4995
BauI	CACGAG	1 cut(s)	5885
BbrPI	CACGTG	2 cut(s)	4454, 5120
BbsI	GAAGAC	4 cut(s)	79, 2339, 5247, 5835
Bbv12I	GWGCWC	3 cut(s)	1650, 1797, 3404
BceAI	ACGGC	3 cut(s)	2277, 3288, 5199
BcgI	CGANNNNNNTGC	2 cut(s)	1520, 1554
BciT130I	CCWGG	8 cut(s)	966, 1560, 2000, 3431, 4046, 5312, 5374, 6436
BciVI	GTATCC	3 cut(s)	1333, 2224, 4545
BclI	TGATCA	4 cut(s)	1285, 4924, 5032, 6292
BcnI	CCSGG	1 cut(s)	5960
BcuI	ACTAGT	1 cut(s)	6277
BfmI	CTRYAG	7 cut(s)	2675, 3340, 3643, 3659, 3902, 4578, 4738
BfoI	RGCGCY	1 cut(s)	6655
BfrI	CTTAAG	2 cut(s)	1690, 3035
BfuAI	ACCTGC	2 cut(s)	1563, 3790
BfuI	GTATCC	3 cut(s)	1333, 2224, 4545
BglII	AGATCT	4 cut(s)	741, 2159, 2594, 2794
BlnI	CCTAGG	1 cut(s)	2614
BlpI	GCTNAGC	1 cut(s)	420
BmcAI	AGTACT	1 cut(s)	5389
Bme1390I	CCNGG	9 cut(s)	966, 1560, 2000, 3431, 4046, 5312, 5374, 5960, 6436
Bme18I	GGWCC	5 cut(s)	4126, 5085, 5197, 5503, 6430
BmgT120I	GGNCC	9 cut(s)	971, 3314, 4126, 4229, 5085, 5197, 5314, 5503, 6430
BmiI	GGNNCC	9 cut(s)	1034, 2336, 3027, 3347, 4690, 5059, 5086, 6419, 6432
BmrFI	CCNGG	9 cut(s)	966, 1560, 2000, 3431, 4046, 5312, 5374, 5960, 6436
BmrI	ACTGGG	5 cut(s)	82, 3147, 3487, 4067, 4431
BmuI	ACTGGG	5 cut(s)	82, 3147, 3487, 4067, 4431
BoxI	GACNNNNGTC	1 cut(s)	5943
BpiI	GAAGAC	4 cut(s)	79, 2339, 5247, 5835
BplI	GAGNNNNNCTC	4 cut(s)	3836, 3868, 3838, 3870
BpmI	CTGGAG	3 cut(s)	987, 5444, 5494
Bpu10I	CCTNAGC	1 cut(s)	723
Bpu1102I	GCTNAGC	1 cut(s)	420
Bpu14I	TTCGAA	1 cut(s)	2167
BpuEI	CTTGAG	6 cut(s)	2045, 2498, 4328, 5048, 6001, 6029
BpuMI	CCSGG	1 cut(s)	5960
BsaAI	YACGTR	5 cut(s)	2411, 2828, 4269, 4454, 5120
BsaBI	GATNNNNATC	1 cut(s)	790
BsaHI	GRCGYC	2 cut(s)	4807, 6770
BsaI	GGTCTC	2 cut(s)	500, 4516
BsaWI	WCCGGW	4 cut(s)	352, 500, 6361, 6403
BsaXI	ACNNNNNCTCC	2 cut(s)	3598, 3628
Bse3DI	GCAATG	4 cut(s)	1365, 4654, 5341, 6634
Bse8I	GATNNNNATC	1 cut(s)	790
BseAI	TCCGGA	2 cut(s)	500, 6403
BseBI	CCWGG	8 cut(s)	966, 1560, 2000, 3431, 4046, 5312, 5374, 6436
BseJI	GATNNNNATC	1 cut(s)	790
BseMI	GCAATG	4 cut(s)	1365, 4654, 5341, 6634
BseRI	GAGGAG	7 cut(s)	2471, 2501, 2881, 2933, 3467, 4358, 4785
BseSI	GKGCMC	4 cut(s)	1797, 2496, 4693, 6420
BseYI	CCCAGC	1 cut(s)	4332
BsgI	GTGCAG	2 cut(s)	2350, 5376
BshFI	GGCC	7 cut(s)	972, 1197, 2304, 3315, 4230, 5315, 6134
BshNI	GGYRCC	2 cut(s)	4688, 6417
BsiHKAI	GWGCWC	3 cut(s)	1650, 1797, 3404
BsiSI	CCGG	8 cut(s)	353, 501, 2426, 4194, 5960, 6362, 6404, 6773
BslFI	GGGAC	6 cut(s)	351, 1678, 3283, 4290, 4818, 5098
BsmBI	CGTCTC	1 cut(s)	6191
BsmFI	GGGAC	6 cut(s)	351, 1678, 3283, 4290, 4818, 5098
BsmI	GAATGC	2 cut(s)	326, 1847
BsnI	GGCC	7 cut(s)	972, 1197, 2304, 3315, 4230, 5315, 6134
Bso31I	GGTCTC	2 cut(s)	500, 4516
Bsp119I	TTCGAA	1 cut(s)	2167
Bsp1286I	GDGCHC	7 cut(s)	1037, 1650, 1797, 2496, 3404, 4693, 6420
Bsp13I	TCCGGA	2 cut(s)	500, 6403
Bsp1407I	TGTACA	5 cut(s)	1054, 1594, 4970, 5617, 6192
Bsp1720I	GCTNAGC	1 cut(s)	420
Bsp19I	CCATGG	2 cut(s)	2539, 6308
BspACI	CCGC	8 cut(s)	115, 765, 974, 1912, 2876, 5434, 6421, 6803
BspANI	GGCC	7 cut(s)	972, 1197, 2304, 3315, 4230, 5315, 6134
BspEI	TCCGGA	2 cut(s)	500, 6403
BspHI	TCATGA	4 cut(s)	1210, 3828, 5029, 5935
BspLI	GGNNCC	9 cut(s)	1034, 2336, 3027, 3347, 4690, 5059, 5086, 6419, 6432
BspMAI	CTGCAG	5 cut(s)	2679, 3344, 3663, 4582, 4742
BspMI	ACCTGC	2 cut(s)	1563, 3790
BspT104I	TTCGAA	1 cut(s)	2167
BspT107I	GGYRCC	2 cut(s)	4688, 6417
BspTI	CTTAAG	2 cut(s)	1690, 3035
BspTNI	GGTCTC	2 cut(s)	500, 4516
BsrDI	GCAATG	4 cut(s)	1365, 4654, 5341, 6634
BsrGI	TGTACA	5 cut(s)	1054, 1594, 4970, 5617, 6192
BssNAI	GTATAC	2 cut(s)	3836, 6182
BssNI	GRCGYC	2 cut(s)	4807, 6770
BssSI	CACGAG	1 cut(s)	5885
BssT1I	CCWWGG	6 cut(s)	2539, 2614, 4859, 5703, 5988, 6308
Bst1107I	GTATAC	2 cut(s)	3836, 6182
Bst2BI	CACGAG	1 cut(s)	5885
Bst2UI	CCWGG	8 cut(s)	966, 1560, 2000, 3431, 4046, 5312, 5374, 6436
Bst6I	CTCTTC	9 cut(s)	231, 1146, 2169, 4808, 5666, 6030, 6167, 6198, 6810
BstACI	GRCGYC	2 cut(s)	4807, 6770
BstAFI	CTTAAG	2 cut(s)	1690, 3035
BstAUI	TGTACA	5 cut(s)	1054, 1594, 4970, 5617, 6192
BstBAI	YACGTR	5 cut(s)	2411, 2828, 4269, 4454, 5120
BstBI	TTCGAA	1 cut(s)	2167
BstC8I	GCNNGC	6 cut(s)	974, 1646, 1697, 5272, 6445, 6455
BstDSI	CCRYGG	3 cut(s)	2539, 5663, 6308
BstH2I	RGCGCY	1 cut(s)	6655
BstHHI	GCGC	3 cut(s)	310, 5165, 6654
BstMWI	GCNNNNNNNGC	5 cut(s)	2639, 2740, 3645, 5345, 5741
BstNI	CCWGG	8 cut(s)	966, 1560, 2000, 3431, 4046, 5312, 5374, 6436
BstNSI	RCATGY	6 cut(s)	512, 1374, 3184, 3944, 5114, 5291
BstPAI	GACNNNNGTC	1 cut(s)	5943
BstSCI	CCNGG	9 cut(s)	964, 1558, 1998, 3429, 4044, 5310, 5372, 5958, 6434
BstSFI	CTRYAG	7 cut(s)	2675, 3340, 3643, 3659, 3902, 4578, 4738
BstSLI	GKGCMC	4 cut(s)	1797, 2496, 4693, 6420
BstV2I	GAAGAC	4 cut(s)	79, 2339, 5247, 5835
BstXI	CCANNNNNNTGG	7 cut(s)	1999, 4052, 4173, 4209, 5207, 5278, 6705
BstZ17I	GTATAC	2 cut(s)	3836, 6182
BsuI	GTATCC	3 cut(s)	1333, 2224, 4545
BsuRI	GGCC	7 cut(s)	972, 1197, 2304, 3315, 4230, 5315, 6134
BtgI	CCRYGG	3 cut(s)	2539, 5663, 6308
BtsI	GCAGTG	1 cut(s)	2672
BtsIMutI	CAGTG	7 cut(s)	868, 893, 1312, 2378, 2672, 5814, 6118
BveI	ACCTGC	2 cut(s)	1563, 3790
Cac8I	GCNNGC	6 cut(s)	974, 1646, 1697, 5272, 6445, 6455
CaiI	CAGNNNCTG	1 cut(s)	2683
CciI	TCATGA	4 cut(s)	1210, 3828, 5029, 5935
CfoI	GCGC	3 cut(s)	310, 5165, 6654
Cfr13I	GGNCC	9 cut(s)	971, 3314, 4126, 4229, 5085, 5197, 5314, 5503, 6430
CseI	GACGC	4 cut(s)	5340, 5933, 6711, 6778
CspCI	CAANNNNNGTGG	2 cut(s)	6459, 6494
DraI	TTTAAA	3 cut(s)	1873, 2820, 4533
DraIII	CACNNNGTG	1 cut(s)	4457
Eam1104I	CTCTTC	9 cut(s)	231, 1146, 2169, 4808, 5666, 6030, 6167, 6198, 6810
EarI	CTCTTC	9 cut(s)	231, 1146, 2169, 4808, 5666, 6030, 6167, 6198, 6810
EciI	GGCGGA	1 cut(s)	2865
Eco130I	CCWWGG	6 cut(s)	2539, 2614, 4859, 5703, 5988, 6308
Eco147I	AGGCCT	1 cut(s)	2304
Eco24I	GRGCYC	1 cut(s)	1037
Eco31I	GGTCTC	2 cut(s)	500, 4516
Eco32I	GATATC	2 cut(s)	2873, 4303
Eco47I	GGWCC	5 cut(s)	4126, 5085, 5197, 5503, 6430
Eco47III	AGCGCT	1 cut(s)	6653
Eco72I	CACGTG	2 cut(s)	4454, 5120
EcoO109I	RGGNCCY	2 cut(s)	5085, 6430
EcoRI	GAATTC	3 cut(s)	938, 3291, 4197
EcoRII	CCWGG	8 cut(s)	964, 1558, 1998, 3429, 4044, 5310, 5372, 6434
EcoRV	GATATC	2 cut(s)	2873, 4303
EcoT14I	CCWWGG	6 cut(s)	2539, 2614, 4859, 5703, 5988, 6308
EcoT22I	ATGCAT	2 cut(s)	1506, 3306
EcoT38I	GRGCYC	1 cut(s)	1037
ErhI	CCWWGG	6 cut(s)	2539, 2614, 4859, 5703, 5988, 6308
Esp3I	CGTCTC	1 cut(s)	6191
FalI	AAGNNNNNCTT	2 cut(s)	3615, 3647
FaqI	GGGAC	6 cut(s)	351, 1678, 3283, 4290, 4818, 5098
FauI	CCCGC	2 cut(s)	122, 981
FauNDI	CATATG	1 cut(s)	4429
FbaI	TGATCA	4 cut(s)	1285, 4924, 5032, 6292
FblI	GTMKAC	3 cut(s)	1006, 3835, 6181
FriOI	GRGCYC	1 cut(s)	1037
FspI	TGCGCA	1 cut(s)	309
GlaI	GCGC	3 cut(s)	309, 5164, 6653
GsaI	CCCAGC	1 cut(s)	4336
GsuI	CTGGAG	3 cut(s)	987, 5444, 5494
HaeII	RGCGCY	1 cut(s)	6655
HaeIII	GGCC	7 cut(s)	972, 1197, 2304, 3315, 4230, 5315, 6134
HapII	CCGG	8 cut(s)	353, 501, 2426, 4194, 5960, 6362, 6404, 6773
HgaI	GACGC	4 cut(s)	5340, 5933, 6711, 6778
HhaI	GCGC	3 cut(s)	310, 5165, 6654
Hin1I	GRCGYC	2 cut(s)	4807, 6770
Hin6I	GCGC	3 cut(s)	308, 5163, 6652
HinP1I	GCGC	3 cut(s)	308, 5163, 6652
HincII	GTYRAC	4 cut(s)	1007, 1565, 4750, 6275
HindII	GTYRAC	4 cut(s)	1007, 1565, 4750, 6275
HindIII	AAGCTT	6 cut(s)	188, 474, 1101, 1321, 1693, 2021
HpaI	GTTAAC	1 cut(s)	4750
HpaII	CCGG	8 cut(s)	353, 501, 2426, 4194, 5960, 6362, 6404, 6773
HphI	GGTGA	8 cut(s)	54, 204, 2060, 2672, 2701, 3071, 4058, 4865
Hpy99I	CGWCG	2 cut(s)	5334, 6705
HpyF10VI	GCNNNNNNNGC	5 cut(s)	2639, 2740, 3645, 5345, 5741
Hsp92I	GRCGYC	2 cut(s)	4807, 6770
HspAI	GCGC	3 cut(s)	308, 5163, 6652
Kpn2I	TCCGGA	2 cut(s)	500, 6403
Ksp22I	TGATCA	4 cut(s)	1285, 4924, 5032, 6292
KspAI	GTTAAC	1 cut(s)	4750
LmnI	GCTCC	6 cut(s)	1032, 2272, 2458, 2639, 3454, 5419
MfeI	CAATTG	4 cut(s)	3554, 5362, 5427, 5498
MhlI	GDGCHC	7 cut(s)	1037, 1650, 1797, 2496, 3404, 4693, 6420
MmeI	TCCRAC	6 cut(s)	2113, 2843, 3009, 3124, 4199, 5972
Mph1103I	ATGCAT	2 cut(s)	1506, 3306
MroI	TCCGGA	2 cut(s)	500, 6403
MroXI	GAANNNNTTC	6 cut(s)	189, 540, 1104, 1127, 2931, 4817
MspA1I	CMGCKG	2 cut(s)	1111, 6447
MspCI	CTTAAG	2 cut(s)	1690, 3035
MspI	CCGG	8 cut(s)	353, 501, 2426, 4194, 5960, 6362, 6404, 6773
MspR9I	CCNGG	9 cut(s)	966, 1560, 2000, 3431, 4046, 5312, 5374, 5960, 6436
MunI	CAATTG	4 cut(s)	3554, 5362, 5427, 5498
Mva1269I	GAATGC	2 cut(s)	326, 1847
MvaI	CCWGG	8 cut(s)	966, 1560, 2000, 3431, 4046, 5312, 5374, 6436
MwoI	GCNNNNNNNGC	5 cut(s)	2639, 2740, 3645, 5345, 5741
NciI	CCSGG	1 cut(s)	5960
NcoI	CCATGG	2 cut(s)	2539, 6308
NdeI	CATATG	1 cut(s)	4429
NlaIV	GGNNCC	9 cut(s)	1034, 2336, 3027, 3347, 4690, 5059, 5086, 6419, 6432
NmuCI	GTSAC	7 cut(s)	511, 2397, 3249, 3782, 4237, 5975, 6482
NsbI	TGCGCA	1 cut(s)	309
NsiI	ATGCAT	2 cut(s)	1506, 3306
NspI	RCATGY	6 cut(s)	512, 1374, 3184, 3944, 5114, 5291
NspV	TTCGAA	1 cut(s)	2167
OliI	CACNNNNGTG	1 cut(s)	4171
PagI	TCATGA	4 cut(s)	1210, 3828, 5029, 5935
PasI	CCCWGGG	1 cut(s)	6435
PceI	AGGCCT	1 cut(s)	2304
PciI	ACATGT	4 cut(s)	3180, 3940, 5110, 5287
PctI	GAATGC	2 cut(s)	326, 1847
PdmI	GAANNNNTTC	6 cut(s)	189, 540, 1104, 1127, 2931, 4817
PflFI	GACNNNGTC	2 cut(s)	4423, 6779
PflMI	CCANNNNNTGG	5 cut(s)	2095, 2296, 3089, 3106, 4457
PmaCI	CACGTG	2 cut(s)	4454, 5120
PmlI	CACGTG	2 cut(s)	4454, 5120
Ppu21I	YACGTR	5 cut(s)	2411, 2828, 4269, 4454, 5120
PpuMI	RGGWCCY	2 cut(s)	5085, 6430
PscI	ACATGT	4 cut(s)	3180, 3940, 5110, 5287
PshAI	GACNNNNGTC	1 cut(s)	5943
PshBI	ATTAAT	1 cut(s)	5181
PsiI	TTATAA	1 cut(s)	1016
Psp1406I	AACGTT	1 cut(s)	5574
Psp5II	RGGWCCY	2 cut(s)	5085, 6430
Psp6I	CCWGG	8 cut(s)	964, 1558, 1998, 3429, 4044, 5310, 5372, 6434
PspCI	CACGTG	2 cut(s)	4454, 5120
PspFI	CCCAGC	1 cut(s)	4332
PspGI	CCWGG	8 cut(s)	964, 1558, 1998, 3429, 4044, 5310, 5372, 6434
PspN4I	GGNNCC	9 cut(s)	1034, 2336, 3027, 3347, 4690, 5059, 5086, 6419, 6432
PspPI	GGNCC	9 cut(s)	971, 3314, 4126, 4229, 5085, 5197, 5314, 5503, 6430
PspPPI	RGGWCCY	2 cut(s)	5085, 6430
PsrI	GAACNNNNNNTAC	2 cut(s)	5106, 5138
PstI	CTGCAG	5 cut(s)	2679, 3344, 3663, 4582, 4742
PstNI	CAGNNNCTG	1 cut(s)	2683
PsyI	GACNNNGTC	2 cut(s)	4423, 6779
PvuII	CAGCTG	2 cut(s)	1111, 6447
SalI	GTCGAC	1 cut(s)	1005
Sau96I	GGNCC	9 cut(s)	971, 3314, 4126, 4229, 5085, 5197, 5314, 5503, 6430
ScaI	AGTACT	1 cut(s)	5389
ScrFI	CCNGG	9 cut(s)	966, 1560, 2000, 3431, 4046, 5312, 5374, 5960, 6436
SduI	GDGCHC	7 cut(s)	1037, 1650, 1797, 2496, 3404, 4693, 6420
SfcI	CTRYAG	7 cut(s)	2675, 3340, 3643, 3659, 3902, 4578, 4738
SfuI	TTCGAA	1 cut(s)	2167
SinI	GGWCC	5 cut(s)	4126, 5085, 5197, 5503, 6430
SmiI	ATTTAAAT	1 cut(s)	2820
SmlI	CTYRAG	8 cut(s)	1690, 2024, 2513, 3035, 4307, 5063, 5980, 6044
SmoI	CTYRAG	8 cut(s)	1690, 2024, 2513, 3035, 4307, 5063, 5980, 6044
SpeI	ACTAGT	1 cut(s)	6277
SseBI	AGGCCT	1 cut(s)	2304
SsiI	CCGC	8 cut(s)	115, 765, 974, 1912, 2876, 5434, 6421, 6803
SspI	AATATT	5 cut(s)	988, 1356, 2476, 5779, 6301
StuI	AGGCCT	1 cut(s)	2304
StyD4I	CCNGG	9 cut(s)	964, 1558, 1998, 3429, 4044, 5310, 5372, 5958, 6434
StyI	CCWWGG	6 cut(s)	2539, 2614, 4859, 5703, 5988, 6308
SwaI	ATTTAAAT	1 cut(s)	2820
TaqII	GACCGA	2 cut(s)	3770, 4143
TauI	GCSGC	3 cut(s)	768, 1914, 5437
TscAI	CASTG	7 cut(s)	868, 900, 1312, 2385, 2679, 5814, 6118
TseFI	GTSAC	7 cut(s)	511, 2397, 3249, 3782, 4237, 5975, 6482
Tsp45I	GTSAC	7 cut(s)	511, 2397, 3249, 3782, 4237, 5975, 6482
TspGWI	ACGGA	8 cut(s)	580, 1388, 2790, 3619, 4225, 5070, 6119, 6260
TspRI	CASTG	7 cut(s)	868, 900, 1312, 2385, 2679, 5814, 6118
Tth111I	GACNNNGTC	2 cut(s)	4423, 6779
Van91I	CCANNNNNTGG	5 cut(s)	2095, 2296, 3089, 3106, 4457
Vha464I	CTTAAG	2 cut(s)	1690, 3035
VneI	GTGCAC	1 cut(s)	1793
VpaK11BI	GGWCC	5 cut(s)	4126, 5085, 5197, 5503, 6430
VspI	ATTAAT	1 cut(s)	5181
XbaI	TCTAGA	4 cut(s)	655, 1235, 2128, 3139
XceI	RCATGY	6 cut(s)	512, 1374, 3184, 3944, 5114, 5291
XmaJI	CCTAGG	1 cut(s)	2614
XmiI	GTMKAC	3 cut(s)	1006, 3835, 6181
XmnI	GAANNNNTTC	6 cut(s)	189, 540, 1104, 1127, 2931, 4817
ZraI	GACGTC	1 cut(s)	4808
ZrmI	AGTACT	1 cut(s)	5389
Zsp2I	ATGCAT	2 cut(s)	1506, 3306

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.