Rorug04G0057800

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002197
Physical Location & Seq
Reverse (-)
9045034 .. 9050560
5527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0057800.1

Sequence Viewer

Length: 3342 bp
ATGTCCAGAAAATTTGTATCAAGGAGGGCTTCGGAATTGGAGTTTGCAGGAATCTTACTTGCTCTATTTATACTTGTTTGCACTTCAGAGGGGTTGAATTCTGAAGGGCTTTACCTTTTAGAGCTGAAGAAGAGTATTTTGGATGAAGCAAATAATTTGGGAAGTTGGAACTCTGCTGATCAAACACCCTGCGGATGGATGGGTGTGAATTGCACTTCTGGTTATGAGCCACTGGTCCAAGGTCTTGATTTGAAATCCATGAATCTTTCAGGAACCCTGAGCCCTAGCATTGGTGGTTTACTTCACCTAACCTCACTTGATCTTGCATCCAATGGTTTCTCTGGAGGCATACCCAAGGAGATTGGAAACTTCTCAAGTTTGGAATACCTTTATCTCAATGACAACAAGTTTACTGGCCAAATACCTGCTGAATTGGGTAAGCTGTCCAAATTAAGAAGTTTGAATCTCTGCAACAACAAAATCTCTGGTCCTCTTCCTGAGGAACTTGGGAACTTGTCTTCACTGGTTGAGTTTGTGGCATACACCAACAATATTACTGGTTCAATACCTCATTCTTTTGGGAATCTCAAAAGTTTGGTAACTTTTCGAGCTGGGCAGAATGCTATTTCTGGTAGCATACCAGCTGAAATAGGTGGATGTCAGAACTTGAAACTGCTTGGTCTTGCTCAAAATGTTATAGGAGGGGAGTTACCTAAGGAGCTTGGGATGCTTGGGAGCATGACAGATCTGATTCTGTGGGATAATCAGGTTTCGGGGTTTATTCCTAAGGAGCTTGGAAATTGCAGTAGTCTTGGGACGATTGCTTTGTATGAGAACAATCTCGTGGGGTCAATACCTCCAGAAATTGGGAATCTCAAGTCATTGAGGAGGTTGTACCTCTACAGGAATGGCTTGAATGGAACCATTCCCAGGGAAATTGGAAATCTTTCTTTTGCTGCTGAGATTGATTTTTCGGAGAACTATTTGATGGGTGAGATCCCATATGAGTTGAGTAAGATAAGTGGTCTAAGCTTACTGTACCTTTTCCAGAACCAGCTCACAGGTGTGATTCCAAATGAGCTTAGTAACTTGAGGAAATTATCAAAGCTTGACCTTTCAATTAATCACCTTGAAGGTCTTATTCCATATGGGTTTCAATATTTGACCGAATTGAGCCAGTTACAGTTGTTCGATAACTCTCTAAGGGGTAGCATACCCCTGTGGCTTGGGCGACACAGTCGACTTTGGGTAGTTGATCTTTCAGATAACTCCCTAACTGGGAGAATACCTCCGTACCTTTGTTGGCATTCCAATCTAATCTTGTTGAACCTGGAGTCTAATGATCTTTATGGAAGTATTCCAACTGGAGTTTTGAACTGTGAATCATTGGTGCAACTTCGCCTTGTTGGAAACAGGCTTACTGGCAGCTTTCCTTCTGAGTTATGCAATTTGGTGAACCTTTCTGCGATTGAGTTGGACGGGAACAAGTTCACTGGGTCTATTCCTCCAGAGATTAAAAACTGCCAAAAGTTGCAAAGGCTTCACATTGCAGACAATTACTTCACATCCGAGTTGCCTAAGGAAATAGGATATCTATCTCAATTGGTAACTTTCAACATATCATCCAATTCATTCACTGGGCAAATTCCGCCTGAAATTGTTAACTGCAAGATGCTTCAGCGACTTGACCTCAGTCGGAATAGATTCGAAAATGCTTTGCCAAATGAGCTGGGAACGCTTTTGCAGTTGGAGCTTCTGAGGCTTTCAGAAAATAGTTTTACTGGAAACATACCAGCAGCACTAGGAAACCTCTCACATTTGACAGAGTTGCAGATGGGTGGCAACTTGTTTTCTGGTGAAATACCACCTGAATTGGGTTCTCTTTCAAGCTTGCAGATTGCAATGAATCTCAGTTTTAATAATCTCAGCGGGAGAATACCACCAGAGCTTGGAAATCTAATTTTACTTGAATTCCTTCTGCTCAATAACAATAATTTGACCGGTGAAATTCCCAGCACATTTGAAAATTTGTCAAGCTTAATGGGCTGCAACTTCTCTTACAATGACCTTACCGGATCTTTACCTCCCATCCCATTGTTCCAGAACATGGCAATTAGCAGCTTTATTGGGAACAAAGGTCTTTGTGGTGGGCCTCTCGGTGTGTGCAGTGTAAATTCATCTCCAAATTCTGTTCGATCTCTGAATAGAGTGGATACTCCTCGCAGTAAAATCATTACAATAATTGCAGCTGTTGTGGGTGGTATTTCTCTTATTTTGATTGCTGTTATCTTATATTTCATGAGACGTCCAGGTCAGACGGTTCCTTCCATGCAAGATAAGGATACTTTGCCTCCTGATACAGATATATACTTACCTCCAAAGGAGGGGATCACGTTCCAAGACCTGGTTGAGGCGACAAATAACTTTCATGACAGCTATGCTGTTGGAAGGGGAGCTTGTGGAACAGTTTACAAGGCAGTAATGCAATCTGGACTTACTATTGCTGTTAAGAAGCTTTCAGCTAACCGGGAGGGCAACAACATTGAAAACAGTTTCCAGGCCGAGATTTTAACTCTGGGAAATATAAGGCATCGGAATATTGTGAAGCTCTATGGATTTTGTTATCACAAGGGTTCCAACCTACTTCTTTATGAGTACATGGAAAAGGGTAGCTTGGGTGAATTACTTCATGGTGAATCTTGCAGCTTGGAGTGGCCAACTCGTTTCATGATTGCTCTTGGAGCTGCTGAAGGTCTTGCTTATTTACATCATGACTGCAAACCAAGGATTGTCCACCGTGATATAAAGTCCAATAATATTTTGCTAGATGAGAAGTTTGAAGCCCATGTTGGTGACTTTGGTTTGGCAAAAGTGATTGATATGCCTCATTCGAAATCAATGTCAGCAGTTGCAGGATCATATGGATATATTGCTCCTGAGTATGCATACACCATGAAGGTCACAGAAAAATGTGACATCTATAGCTATGGGGTAGTGCTATTGGAGCTGCTAACTGGAAGAACTCCTGTACAATCACTAGATGAAGGAGGTGATCTTGTCACCTGGGTTAGACACTACATCAGACACCATTCTTTGACATCTGGGATACTCGACAGCCGGTTGAATCTTCAAGACAAAAGTATGGTTGATCACATGCTTACCGTCCTAAAGATTGCTTTGATGTGCACTAGTACGTCCCCATTCGACCGTCCATCAATTCGAGAAGTTGTGCTGATGCTTATTGAGTCCAATGAGCAAGAAGGAGACTTTGTGCCATCTCCAACTTATGATCTTCCTTTGACAGATGATTCAGATATTCCATTGAAAGATGATACTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

1113

Amino Acids

122.0

Weight (kDa)

5.3

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 32 - 72 1.3e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 82 - 200 6e-10 Leucine-rich repeat region
LRR_14 PF23598 288 - 394 3.1e-06 Leucine-rich repeat region
LRR_14 PF23598 547 - 633 5.3e-08 Leucine-rich repeat region
Pkinase PF00069 810 - 1077 2.3e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 814 - 1077 1e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2310
AatII GACGTC 1 cut(s) 2308
Acc36I ACCTGC 1 cut(s) 433
AccB7I CCANNNNNTGG 4 cut(s) 866, 1949, 2107, 2404
AccI GTMKAC 1 cut(s) 1240
AciI CCGC 3 cut(s) 192, 1649, 1929
AclWI GGATC 4 cut(s) 991, 2083, 2396, 2923
AcoI YGGCCR 2 cut(s) 415, 2714
AcsI RAATTY 8 cut(s) 11, 97, 1644, 1970, 2007, 2026, 2173, 2185
AcuI CTGAAG 5 cut(s) 69, 123, 146, 1661, 2769
AcyI GRCGYC 1 cut(s) 2305
AfaI GTAC 6 cut(s) 896, 1040, 1295, 2657, 3030, 3193
AgeI ACCGGT 1 cut(s) 2000
AhlI ACTAGT 1 cut(s) 3188
AjnI CCWGG 6 cut(s) 929, 1329, 2308, 2403, 2556, 3062
AjuI GAANNNNNNNTTGG 4 cut(s) 122, 154, 2832, 2864
AleI CACNNNNGTG 1 cut(s) 1064
Alw21I GWGCWC 1 cut(s) 3188
Alw26I GTCTC 2 cut(s) 2296, 3258
Alw44I GTGCAC 1 cut(s) 3184
AlwI GGATC 4 cut(s) 991, 2083, 2396, 2923
AoxI GGCC 4 cut(s) 415, 2150, 2559, 2714
ApaLI GTGCAC 1 cut(s) 3184
ApeKI GCWGC 9 cut(s) 956, 1425, 1796, 2046, 2118, 2246, 2703, 2744, 3007
ApoI RAATTY 8 cut(s) 11, 97, 1644, 1970, 2007, 2026, 2173, 2185
ArsI GACNNNNNNTTYG 4 cut(s) 808, 840, 2885, 2917
AseI ATTAAT 1 cut(s) 1122
AsiGI ACCGGT 1 cut(s) 2000
Asp700I GAANNNNTTC 5 cut(s) 946, 1487, 1703, 1974, 2685
AspS9I GGNCC 3 cut(s) 235, 488, 2150
AsuC2I CCSGG 1 cut(s) 2528
AsuII TTCGAA 2 cut(s) 1707, 2891
AvaII GGWCC 2 cut(s) 235, 488
AxyI CCTNAGG 4 cut(s) 498, 714, 786, 1578
BaeGI GKGCMC 1 cut(s) 3188
BaeI ACNNNNGTAYC 2 cut(s) 1277, 1310
BalI TGGCCA 2 cut(s) 417, 2716
BanII GRGCYC 1 cut(s) 284
BauI CACGAG 1 cut(s) 842
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 3188
BbvI GCAGC 9 cut(s) 943, 1437, 1808, 2033, 2130, 2258, 2715, 2731, 2994
BccI CCATC 7 cut(s) 189, 193, 982, 1828, 2096, 3220, 3283
BciT130I CCWGG 6 cut(s) 931, 1331, 2310, 2405, 2558, 3064
BciVI GTATCC 3 cut(s) 2206, 2335, 3099
BclI TGATCA 2 cut(s) 178, 3148
BcnI CCSGG 1 cut(s) 2528
BcoDI GTCTC 2 cut(s) 2296, 3258
BcuI ACTAGT 1 cut(s) 3188
BfaI CTAG 5 cut(s) 285, 1802, 2825, 3038, 3189
BfmI CTRYAG 2 cut(s) 901, 2980
BfuAI ACCTGC 1 cut(s) 433
BfuI GTATCC 3 cut(s) 2206, 2335, 3099
BglII AGATCT 1 cut(s) 745
BisI GCNGC 9 cut(s) 957, 1426, 1797, 2047, 2119, 2247, 2704, 2745, 3008
BlsI GCNGC 9 cut(s) 958, 1427, 1798, 2048, 2120, 2248, 2705, 2746, 3009
Bme1390I CCNGG 7 cut(s) 931, 1331, 2310, 2405, 2528, 2558, 3064
Bme18I GGWCC 2 cut(s) 235, 488
BmgT120I GGNCC 3 cut(s) 235, 488, 2150
BmiI GGNNCC 4 cut(s) 274, 922, 2322, 2635
BmrFI CCNGG 7 cut(s) 931, 1331, 2310, 2405, 2528, 2558, 3064
BmrI ACTGGG 3 cut(s) 1287, 1503, 1647
BmsI GCATC 5 cut(s) 335, 717, 1662, 2599, 3225
BmuI ACTGGG 3 cut(s) 1287, 1503, 1647
BoxI GACNNNNGTC 1 cut(s) 1692
BpiI GAAGAC 1 cut(s) 510
BplI GAGNNNNNCTC 2 cut(s) 1273, 1305
BpmI CTGGAG 5 cut(s) 363, 843, 1352, 1386, 1491
Bpu10I CCTNAGC 1 cut(s) 278
Bpu14I TTCGAA 2 cut(s) 1707, 2891
BpuEI CTTGAG 3 cut(s) 358, 860, 1111
BpuMI CCSGG 1 cut(s) 2528
BsaBI GATNNNNATC 1 cut(s) 1594
BsaHI GRCGYC 1 cut(s) 2305
BsaJI CCNNGG 6 cut(s) 238, 354, 929, 930, 2783, 3063
BsaWI WCCGGW 2 cut(s) 2000, 2072
BsaXI ACNNNNNCTCC 4 cut(s) 2335, 2365, 3255, 3285
Bse118I RCCGGY 2 cut(s) 2000, 3117
Bse21I CCTNAGG 4 cut(s) 498, 714, 786, 1578
Bse3DI GCAATG 2 cut(s) 1545, 1908
Bse8I GATNNNNATC 1 cut(s) 1594
BseBI CCWGG 6 cut(s) 931, 1331, 2310, 2405, 2558, 3064
BseDI CCNNGG 6 cut(s) 238, 354, 929, 930, 2783, 3063
BseGI GGATG 9 cut(s) 148, 200, 204, 326, 662, 732, 1565, 1622, 2088
BseJI GATNNNNATC 1 cut(s) 1594
BseMI GCAATG 2 cut(s) 1545, 1908
BseMII CTCAG 9 cut(s) 269, 489, 951, 1428, 1705, 1748, 1924, 1939, 2928
BseRI GAGGAG 2 cut(s) 901, 2208
BseSI GKGCMC 1 cut(s) 3188
BseXI GCAGC 9 cut(s) 943, 1437, 1808, 2033, 2130, 2258, 2715, 2731, 2994
BseYI CCCAGC 3 cut(s) 611, 1729, 2012
BsgI GTGCAG 1 cut(s) 2185
Bsh1285I CGRYCG 1 cut(s) 3208
BshFI GGCC 4 cut(s) 417, 2152, 2561, 2716
BshTI ACCGGT 1 cut(s) 2000
BsiEI CGRYCG 1 cut(s) 3208
BsiHKAI GWGCWC 1 cut(s) 3188
BsiSI CCGG 4 cut(s) 2001, 2073, 2527, 3118
BslFI GGGAC 2 cut(s) 829, 3181
BsmAI GTCTC 2 cut(s) 2296, 3258
BsmBI CGTCTC 1 cut(s) 2296
BsmFI GGGAC 2 cut(s) 829, 3181
BsmI GAATGC 2 cut(s) 625, 1306
BsnI GGCC 4 cut(s) 417, 2152, 2561, 2716
Bsp119I TTCGAA 2 cut(s) 1707, 2891
Bsp1286I GDGCHC 2 cut(s) 284, 3188
Bsp1407I TGTACA 1 cut(s) 3028
BspACI CCGC 3 cut(s) 192, 1649, 1929
BspANI GGCC 4 cut(s) 417, 2152, 2561, 2716
BspCNI CTCAG 9 cut(s) 270, 490, 952, 1429, 1704, 1749, 1923, 1938, 2929
BspHI TCATGA 4 cut(s) 2298, 2428, 2727, 2770
BspLI GGNNCC 4 cut(s) 274, 922, 2322, 2635
BspMI ACCTGC 1 cut(s) 433
BspPI GGATC 4 cut(s) 991, 2083, 2396, 2923
BspT104I TTCGAA 2 cut(s) 1707, 2891
BsrDI GCAATG 2 cut(s) 1545, 1908
BsrFI RCCGGY 2 cut(s) 2000, 3117
BsrGI TGTACA 1 cut(s) 3028
BssAI RCCGGY 2 cut(s) 2000, 3117
BssECI CCNNGG 6 cut(s) 238, 354, 929, 930, 2783, 3063
BssNI GRCGYC 1 cut(s) 2305
BssSI CACGAG 1 cut(s) 842
BssT1I CCWWGG 3 cut(s) 238, 354, 2783
Bst2BI CACGAG 1 cut(s) 842
Bst2UI CCWGG 6 cut(s) 931, 1331, 2310, 2405, 2558, 3064
Bst6I CTCTTC 2 cut(s) 125, 498
BstACI GRCGYC 1 cut(s) 2305
BstAUI TGTACA 1 cut(s) 3028
BstBI TTCGAA 2 cut(s) 1707, 2891
BstC8I GCNNGC 1 cut(s) 1892
BstENI CCTNNNNNAGG 1 cut(s) 2408
BstF5I GGATG 9 cut(s) 148, 200, 204, 326, 662, 732, 1565, 1622, 2088
BstMAI GTCTC 2 cut(s) 2296, 3258
BstMCI CGRYCG 1 cut(s) 3208
BstMWI GCNNNNNNNGC 9 cut(s) 727, 1648, 1726, 1735, 1750, 1759, 2043, 2741, 3004
BstNI CCWGG 6 cut(s) 931, 1331, 2310, 2405, 2558, 3064
BstNSI RCATGY 1 cut(s) 3157
BstPAI GACNNNNGTC 1 cut(s) 1692
BstSCI CCNGG 7 cut(s) 929, 1329, 2308, 2403, 2526, 2556, 3062
BstSFI CTRYAG 2 cut(s) 901, 2980
BstSLI GKGCMC 1 cut(s) 3188
BstV1I GCAGC 9 cut(s) 943, 1437, 1808, 2033, 2130, 2258, 2715, 2731, 2994
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 3 cut(s) 745, 996, 2075
BstYI RGATCY 3 cut(s) 745, 996, 2075
Bsu36I CCTNAGG 4 cut(s) 498, 714, 786, 1578
BsuI GTATCC 3 cut(s) 2206, 2335, 3099
BsuRI GGCC 4 cut(s) 417, 2152, 2561, 2716
BtsCI GGATG 9 cut(s) 148, 200, 204, 326, 662, 732, 1565, 1622, 2088
BtsI GCAGTG 1 cut(s) 2173
BtsIMutI CAGTG 5 cut(s) 230, 521, 1491, 1635, 2173
BveI ACCTGC 1 cut(s) 433
Cac8I GCNNGC 1 cut(s) 1892
CciI TCATGA 4 cut(s) 2298, 2428, 2727, 2770
Cfr10I RCCGGY 2 cut(s) 2000, 3117
Cfr13I GGNCC 3 cut(s) 235, 488, 2150
CsiI ACCWGGT 1 cut(s) 2403
Csp6I GTAC 6 cut(s) 895, 1039, 1294, 2656, 3029, 3192
CspAI ACCGGT 1 cut(s) 2000
CspCI CAANNNNNGTGG 2 cut(s) 1854, 1889
CviQI GTAC 6 cut(s) 895, 1039, 1294, 2656, 3029, 3192
DrdI GACNNNNNNGTC 1 cut(s) 2310
DseDI GACNNNNNNGTC 1 cut(s) 2310
EaeI YGGCCR 2 cut(s) 415, 2714
Eam1104I CTCTTC 2 cut(s) 125, 498
EarI CTCTTC 2 cut(s) 125, 498
EciI GGCGGA 1 cut(s) 1638
Eco130I CCWWGG 3 cut(s) 238, 354, 2783
Eco24I GRGCYC 1 cut(s) 284
Eco32I GATATC 1 cut(s) 1592
Eco47I GGWCC 2 cut(s) 235, 488
Eco57I CTGAAG 5 cut(s) 69, 123, 146, 1661, 2769
Eco81I CCTNAGG 4 cut(s) 498, 714, 786, 1578
EcoNI CCTNNNNNAGG 1 cut(s) 2408
EcoRI GAATTC 2 cut(s) 97, 1970
EcoRII CCWGG 6 cut(s) 929, 1329, 2308, 2403, 2556, 3062
EcoRV GATATC 1 cut(s) 1592
EcoT14I CCWWGG 3 cut(s) 238, 354, 2783
EcoT22I ATGCAT 1 cut(s) 2947
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 3 cut(s) 238, 354, 2783
Esp3I CGTCTC 1 cut(s) 2296
FalI AAGNNNNNCTT 6 cut(s) 13, 45, 2440, 2472, 2657, 2689
FaqI GGGAC 2 cut(s) 829, 3181
FauI CCCGC 1 cut(s) 1922
FauNDI CATATG 3 cut(s) 1003, 1147, 2920
FbaI TGATCA 2 cut(s) 178, 3148
FblI GTMKAC 1 cut(s) 1240
Fnu4HI GCNGC 9 cut(s) 957, 1426, 1797, 2047, 2119, 2247, 2704, 2745, 3008
FokI GGATG 9 cut(s) 155, 207, 211, 313, 669, 739, 1552, 1609, 2075
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 9 cut(s) 957, 1426, 1797, 2047, 2119, 2247, 2704, 2745, 3008
FspBI CTAG 5 cut(s) 285, 1802, 2825, 3038, 3189
GluI GCNGC 9 cut(s) 957, 1426, 1797, 2047, 2119, 2247, 2704, 2745, 3008
GsaI CCCAGC 3 cut(s) 615, 1733, 2016
GsuI CTGGAG 5 cut(s) 363, 843, 1352, 1386, 1491
HaeIII GGCC 4 cut(s) 417, 2152, 2561, 2716
HapII CCGG 4 cut(s) 2001, 2073, 2527, 3118
Hin1I GRCGYC 1 cut(s) 2305
HincII GTYRAC 2 cut(s) 1241, 1663
HindII GTYRAC 2 cut(s) 1241, 1663
HindIII AAGCTT 5 cut(s) 1030, 1106, 1888, 2035, 2513
HpaI GTTAAC 1 cut(s) 1663
HpaII CCGG 4 cut(s) 2001, 2073, 2527, 3118
Hpy166II GTNNAC 9 cut(s) 299, 411, 1241, 1456, 1491, 1663, 2470, 2794, 3186
Hpy8I GTNNAC 9 cut(s) 299, 411, 1241, 1456, 1491, 1663, 2470, 2794, 3186
HpyCH4IV ACGT 3 cut(s) 2305, 2393, 3194
HpyF10VI GCNNNNNNNGC 9 cut(s) 727, 1648, 1726, 1735, 1750, 1759, 2043, 2741, 3004
HpySE526I ACGT 3 cut(s) 2305, 2393, 3194
Hsp92I GRCGYC 1 cut(s) 2305
Ksp22I TGATCA 2 cut(s) 178, 3148
KspAI GTTAAC 1 cut(s) 1663
LmnI GCTCC 8 cut(s) 718, 735, 790, 1750, 2453, 2741, 2938, 3004
Lsp1109I GCAGC 9 cut(s) 943, 1437, 1808, 2033, 2130, 2258, 2715, 2731, 2994
LweI GCATC 5 cut(s) 335, 717, 1662, 2599, 3225
MabI ACCWGGT 1 cut(s) 2403
MaeI CTAG 5 cut(s) 285, 1802, 2825, 3038, 3189
MaeII ACGT 3 cut(s) 2305, 2393, 3194
MaeIII GTNAC 9 cut(s) 598, 708, 1085, 1179, 1606, 2852, 2959, 2972, 3058
MboII GAAGA 7 cut(s) 139, 142, 485, 510, 3030, 3119, 3284
MfeI CAATTG 1 cut(s) 1601
MflI RGATCY 3 cut(s) 745, 996, 2075
MhlI GDGCHC 2 cut(s) 284, 3188
MlsI TGGCCA 2 cut(s) 417, 2716
MluNI TGGCCA 2 cut(s) 417, 2716
MlyI GAGTC 2 cut(s) 1343, 3254
MmeI TCCRAC 9 cut(s) 146, 1385, 1387, 1455, 1676, 1728, 2425, 2661, 3305
Mox20I TGGCCA 2 cut(s) 417, 2716
Mph1103I ATGCAT 1 cut(s) 2947
MroXI GAANNNNTTC 5 cut(s) 946, 1487, 1703, 1974, 2685
MscI TGGCCA 2 cut(s) 417, 2716
MseI TTAA 8 cut(s) 452, 1122, 1515, 1662, 1917, 2039, 2508, 2570
MslI CAYNNNNRTG 2 cut(s) 1064, 2850
Msp20I TGGCCA 2 cut(s) 417, 2716
MspA1I CMGCKG 3 cut(s) 644, 1929, 2249
MspI CCGG 4 cut(s) 2001, 2073, 2527, 3118
MspR9I CCNGG 7 cut(s) 931, 1331, 2310, 2405, 2528, 2558, 3064
MunI CAATTG 1 cut(s) 1601
Mva1269I GAATGC 2 cut(s) 625, 1306
MvaI CCWGG 6 cut(s) 931, 1331, 2310, 2405, 2558, 3064
MwoI GCNNNNNNNGC 9 cut(s) 727, 1648, 1726, 1735, 1750, 1759, 2043, 2741, 3004
NciI CCSGG 1 cut(s) 2528
NdeI CATATG 3 cut(s) 1003, 1147, 2920
NlaIV GGNNCC 4 cut(s) 274, 922, 2322, 2635
NmeAIII GCCGAG 1 cut(s) 2587
NmuCI GTSAC 4 cut(s) 2852, 2959, 2972, 3058
NsiI ATGCAT 1 cut(s) 2947
NspI RCATGY 1 cut(s) 3157
NspV TTCGAA 2 cut(s) 1707, 2891
OliI CACNNNNGTG 1 cut(s) 1064
PagI TCATGA 4 cut(s) 2298, 2428, 2727, 2770
PasI CCCWGGG 1 cut(s) 930
PctI GAATGC 2 cut(s) 625, 1306
PdmI GAANNNNTTC 5 cut(s) 946, 1487, 1703, 1974, 2685
PflFI GACNNNGTC 1 cut(s) 1236
PflMI CCANNNNNTGG 4 cut(s) 866, 1949, 2107, 2404
PinAI ACCGGT 1 cut(s) 2000
PkrI GCNGC 9 cut(s) 958, 1427, 1798, 2048, 2120, 2248, 2705, 2746, 3009
PleI GAGTC 2 cut(s) 1342, 3253
PpsI GAGTC 2 cut(s) 1342, 3253
PshAI GACNNNNGTC 1 cut(s) 1692
PshBI ATTAAT 1 cut(s) 1122
Psp6I CCWGG 6 cut(s) 929, 1329, 2308, 2403, 2556, 3062
PspFI CCCAGC 3 cut(s) 611, 1729, 2012
PspGI CCWGG 6 cut(s) 929, 1329, 2308, 2403, 2556, 3062
PspN4I GGNNCC 4 cut(s) 274, 922, 2322, 2635
PspPI GGNCC 3 cut(s) 235, 488, 2150
PsuI RGATCY 3 cut(s) 745, 996, 2075
PsyI GACNNNGTC 1 cut(s) 1236
PvuII CAGCTG 2 cut(s) 644, 2249
RsaI GTAC 6 cut(s) 896, 1040, 1295, 2657, 3030, 3193
RsaNI GTAC 6 cut(s) 895, 1039, 1294, 2656, 3029, 3192
RseI CAYNNNNRTG 2 cut(s) 1064, 2850
SalI GTCGAC 1 cut(s) 1239
SaqAI TTAA 8 cut(s) 452, 1122, 1515, 1662, 1917, 2039, 2508, 2570
SatI GCNGC 9 cut(s) 957, 1426, 1797, 2047, 2119, 2247, 2704, 2745, 3008
Sau96I GGNCC 3 cut(s) 235, 488, 2150
SchI GAGTC 2 cut(s) 1343, 3254
ScrFI CCNGG 7 cut(s) 931, 1331, 2310, 2405, 2528, 2558, 3064
SduI GDGCHC 2 cut(s) 284, 3188
SexAI ACCWGGT 1 cut(s) 2403
SfaNI GCATC 5 cut(s) 335, 717, 1662, 2599, 3225
SfcI CTRYAG 2 cut(s) 901, 2980
SfuI TTCGAA 2 cut(s) 1707, 2891
SinI GGWCC 2 cut(s) 235, 488
SmiMI CAYNNNNRTG 2 cut(s) 1064, 2850
SmlI CTYRAG 3 cut(s) 373, 875, 1090
SmoI CTYRAG 3 cut(s) 373, 875, 1090
SpeI ACTAGT 1 cut(s) 3188
SsiI CCGC 3 cut(s) 192, 1649, 1929
SspI AATATT 4 cut(s) 553, 1160, 2599, 2818
SspMI CTAG 5 cut(s) 285, 1802, 2825, 3038, 3189
StyD4I CCNGG 7 cut(s) 929, 1329, 2308, 2403, 2526, 2556, 3062
StyI CCWWGG 3 cut(s) 238, 354, 2783
TaiI ACGT 3 cut(s) 2308, 2396, 3197
TaqII GACCGA 1 cut(s) 1181
TatI WGTACW 2 cut(s) 2655, 3028
Tru1I TTAA 8 cut(s) 452, 1122, 1515, 1662, 1917, 2039, 2508, 2570
Tru9I TTAA 8 cut(s) 452, 1122, 1515, 1662, 1917, 2039, 2508, 2570
TscAI CASTG 5 cut(s) 237, 528, 1498, 1642, 2173
TseFI GTSAC 4 cut(s) 2852, 2959, 2972, 3058
TseI GCWGC 9 cut(s) 956, 1425, 1796, 2046, 2118, 2246, 2703, 2744, 3007
Tsp45I GTSAC 4 cut(s) 2852, 2959, 2972, 3058
TspGWI ACGGA 1 cut(s) 1281
TspRI CASTG 5 cut(s) 237, 528, 1498, 1642, 2173
Tth111I GACNNNGTC 1 cut(s) 1236
Van91I CCANNNNNTGG 4 cut(s) 866, 1949, 2107, 2404
VneI GTGCAC 1 cut(s) 3184
VpaK11BI GGWCC 2 cut(s) 235, 488
VspI ATTAAT 1 cut(s) 1122
XagI CCTNNNNNAGG 1 cut(s) 2408
XapI RAATTY 8 cut(s) 11, 97, 1644, 1970, 2007, 2026, 2173, 2185
XceI RCATGY 1 cut(s) 3157
XmiI GTMKAC 1 cut(s) 1240
XmnI GAANNNNTTC 5 cut(s) 946, 1487, 1703, 1974, 2685
XspI CTAG 5 cut(s) 285, 1802, 2825, 3038, 3189
ZraI GACGTC 1 cut(s) 2306
Zsp2I ATGCAT 1 cut(s) 2947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.