Rh4AG131300

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002197
Physical Location & Seq
Reverse (-)
28475161 .. 28477537
2377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG131300.1

Sequence Viewer

Length: 1044 bp
ATGGCCAAGTTATCACCTCAGAACCAGAAAACTAATGCAAGTAATAATGCAACTACTACTGCTGCTGTTAATAATGCAAATGCAGCTAATAAAACAGTAGTCACAAAGGTGAAACGAACCAGGAGAAGTGTGCCGAGGGACTCCCCACCTCAACGCAGCTCCATTTATCGAGGCGTCACCAGGTTCCTAAACGAGGTTTTCTCTGCGGTTACTTGTTCAGGCATCGATGGACTGGCCGATATGAGGCTCATCTGTGGGACAAGAATTGCTGGAATGAATCACAAAACAAGAAAGGCCGCCAAGTTAGCTACATATCACAAAGAAATGAATGATATGGAAGGCCAATCAAGAGAGGAATATATTGGATCCTTGAGAAGGAAGAGCAGTGGTTTTTCTCGTGGAGTTTCCAAATACAGAGGTGTTGCTAGACATCATCACAATGGAAGATGGGAAGCTCGAATTGGCCGGGTCTTTGGAAACAAATATCTCTATCTAGGGACATATGCTACCCAAGAAGAGGCTGCAACAGCTTATGACATGGCAGCTATAGAGTATCGTGGTCTCAATGCAGTTACCAACTTTGATCTCAGCCGTTACATCAAATGGCTAAAACCAAATGGTCCTAACAATGACATTATTGCCCAGACCACTAACCCTAACGTTGAAGCTAAATTGGTTGGAGATGTAACCCAAAACCCTAATGATCAAGACCAAACTGGACTAGGTTTCTTCCACAACCTCGCATACAGTCCTCCTGAAGAAATCACAATGACCCAGTTGCCTCGGCCGACCACTGCTACTTCAGCCCTAGGACTCCTGCTTCAGTCATCAAAGTTCAAGGAGATGATGGAGATGACAACGGCCACCGACTCATCCATGACATCGTCATCCCTGCCGGAACTGCCTCAGTGCACTTTTCCCGATGACATACAGACTTACTTCGACTGCCAAGATTCTACGAGCTATGGAGATGGAGACGATGCTATGTTCAGTGAGCTCAACTCATACATCCCGTCCATGTTTCAATGCGATTTCACCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

347

Amino Acids

38.51

Weight (kDa)

8.68

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 136 - 187 1.9e-11 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 206, 297
AclI AACGTT 1 cut(s) 660
AclWI GGATC 2 cut(s) 360, 373
AcoI YGGCCR 5 cut(s) 3, 234, 463, 785, 861
AcuI CTGAAG 3 cut(s) 777, 786, 806
AcyI GRCGYC 1 cut(s) 174
AfiI CCNNNNNNNGG 4 cut(s) 193, 243, 375, 517
AgsI TTSAA 3 cut(s) 665, 838, 1025
AjnI CCWGG 2 cut(s) 119, 179
AjuI GAANNNNNNNTTGG 2 cut(s) 444, 476
AleI CACNNNNGTG 1 cut(s) 107
AluBI AGCT 9 cut(s) 86, 159, 308, 455, 530, 545, 668, 963, 997
AluI AGCT 9 cut(s) 86, 159, 308, 455, 530, 545, 668, 963, 997
Alw21I GWGCWC 2 cut(s) 914, 999
Alw26I GTCTC 2 cut(s) 566, 969
Alw44I GTGCAC 1 cut(s) 910
AlwI GGATC 2 cut(s) 360, 373
AoxI GGCC 7 cut(s) 3, 234, 294, 340, 463, 785, 861
ApaLI GTGCAC 1 cut(s) 910
ApeKI GCWGC 5 cut(s) 62, 83, 156, 521, 542
AspA2I CCTAGG 1 cut(s) 808
AspS9I GGNCC 1 cut(s) 620
AsuC2I CCSGG 1 cut(s) 467
AsuHPI GGTGA 4 cut(s) 6, 121, 169, 1027
AvaII GGWCC 1 cut(s) 620
AvrII CCTAGG 1 cut(s) 808
BaeGI GKGCMC 1 cut(s) 914
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 365
BanII GRGCYC 1 cut(s) 999
BauI CACGAG 1 cut(s) 396
Bbv12I GWGCWC 2 cut(s) 914, 999
BbvI GCAGC 5 cut(s) 49, 95, 168, 508, 554
BccI CCATC 4 cut(s) 221, 441, 841, 965
BceAI ACGGC 2 cut(s) 576, 876
BciT130I CCWGG 2 cut(s) 121, 181
BclI TGATCA 1 cut(s) 703
BcnI CCSGG 1 cut(s) 467
BcoDI GTCTC 2 cut(s) 566, 969
BfaI CTAG 5 cut(s) 426, 494, 722, 809, 1042
BfmI CTRYAG 1 cut(s) 546
BisI GCNGC 6 cut(s) 63, 84, 157, 297, 522, 543
BlnI CCTAGG 1 cut(s) 808
BlsI GCNGC 6 cut(s) 64, 85, 158, 298, 523, 544
Bme1390I CCNGG 3 cut(s) 121, 181, 467
Bme18I GGWCC 1 cut(s) 620
BmgT120I GGNCC 1 cut(s) 620
BmiI GGNNCC 2 cut(s) 185, 367
BmrFI CCNGG 3 cut(s) 121, 181, 467
BmrI ACTGGG 1 cut(s) 769
BmsI GCATC 2 cut(s) 231, 970
BmuI ACTGGG 1 cut(s) 769
BplI GAGNNNNNCTC 4 cut(s) 185, 217, 986, 1018
BpuEI CTTGAG 1 cut(s) 391
BpuMI CCSGG 1 cut(s) 467
Bsa29I ATCGAT 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 566
BsaJI CCNNGG 3 cut(s) 134, 782, 808
Bsc4I CCNNNNNNNGG 4 cut(s) 193, 243, 375, 517
Bse1I ACTGG 3 cut(s) 237, 721, 775
BseBI CCWGG 2 cut(s) 121, 181
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 3 cut(s) 134, 782, 808
BseGI GGATG 3 cut(s) 872, 887, 1008
BseLI CCNNNNNNNGG 4 cut(s) 193, 243, 375, 517
BseMII CTCAG 3 cut(s) 32, 601, 920
BseNI ACTGG 3 cut(s) 237, 721, 775
BseSI GKGCMC 1 cut(s) 914
BseX3I CGGCCG 1 cut(s) 785
BseXI GCAGC 5 cut(s) 49, 95, 168, 508, 554
Bsh1285I CGRYCG 1 cut(s) 788
BshFI GGCC 7 cut(s) 5, 236, 296, 342, 465, 787, 863
BshVI ATCGAT 1 cut(s) 225
BsiEI CGRYCG 1 cut(s) 788
BsiHKAI GWGCWC 2 cut(s) 914, 999
BsiSI CCGG 2 cut(s) 466, 896
BslFI GGGAC 3 cut(s) 152, 271, 511
BslI CCNNNNNNNGG 4 cut(s) 193, 243, 375, 517
BsmAI GTCTC 2 cut(s) 566, 969
BsmBI CGTCTC 1 cut(s) 969
BsmFI GGGAC 3 cut(s) 152, 271, 511
BsnI GGCC 7 cut(s) 5, 236, 296, 342, 465, 787, 863
Bso31I GGTCTC 1 cut(s) 566
Bsp1286I GDGCHC 2 cut(s) 914, 999
Bsp143I GATC 3 cut(s) 365, 583, 703
BspACI CCGC 2 cut(s) 206, 297
BspANI GGCC 7 cut(s) 5, 236, 296, 342, 465, 787, 863
BspCNI CTCAG 3 cut(s) 31, 600, 919
BspDI ATCGAT 1 cut(s) 225
BspLI GGNNCC 2 cut(s) 185, 367
BspPI GGATC 2 cut(s) 360, 373
BspQI GCTCTTC 1 cut(s) 374
BspTNI GGTCTC 1 cut(s) 566
BsrI ACTGG 3 cut(s) 237, 721, 775
BssECI CCNNGG 3 cut(s) 134, 782, 808
BssMI GATC 3 cut(s) 365, 583, 703
BssNI GRCGYC 1 cut(s) 174
BssSI CACGAG 1 cut(s) 396
BssT1I CCWWGG 1 cut(s) 808
Bst2BI CACGAG 1 cut(s) 396
Bst2UI CCWGG 2 cut(s) 121, 181
Bst4CI ACNGT 3 cut(s) 97, 749, 1039
Bst6I CTCTTC 2 cut(s) 374, 510
BstACI GRCGYC 1 cut(s) 174
BstDEI CTNAG 3 cut(s) 18, 587, 906
BstENI CCTNNNNNAGG 2 cut(s) 191, 373
BstF5I GGATG 3 cut(s) 872, 887, 1008
BstKTI GATC 3 cut(s) 368, 586, 706
BstMAI GTCTC 2 cut(s) 566, 969
BstMBI GATC 3 cut(s) 365, 583, 703
BstMCI CGRYCG 1 cut(s) 788
BstMWI GCNNNNNNNGC 5 cut(s) 83, 305, 527, 803, 901
BstNI CCWGG 2 cut(s) 121, 181
BstSCI CCNGG 3 cut(s) 119, 179, 465
BstSFI CTRYAG 1 cut(s) 546
BstSLI GKGCMC 1 cut(s) 914
BstV1I GCAGC 5 cut(s) 49, 95, 168, 508, 554
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
BstZI CGGCCG 1 cut(s) 785
Bsu15I ATCGAT 1 cut(s) 225
BsuRI GGCC 7 cut(s) 5, 236, 296, 342, 465, 787, 863
BsuTUI ATCGAT 1 cut(s) 225
BtsCI GGATG 3 cut(s) 872, 887, 1008
BtsI GCAGTG 2 cut(s) 391, 792
BtsIMutI CAGTG 4 cut(s) 391, 792, 914, 997
Cfr13I GGNCC 1 cut(s) 620
ClaI ATCGAT 1 cut(s) 225
CseI GACGC 1 cut(s) 163
CsiI ACCWGGT 1 cut(s) 179
CviAII CATG 3 cut(s) 538, 877, 1018
DdeI CTNAG 3 cut(s) 18, 587, 906
DpnI GATC 3 cut(s) 367, 585, 705
DpnII GATC 3 cut(s) 365, 583, 703
EaeI YGGCCR 5 cut(s) 3, 234, 463, 785, 861
EagI CGGCCG 1 cut(s) 785
Eam1104I CTCTTC 2 cut(s) 374, 510
EarI CTCTTC 2 cut(s) 374, 510
Ecl136II GAGCTC 1 cut(s) 997
EclXI CGGCCG 1 cut(s) 785
Eco130I CCWWGG 1 cut(s) 808
Eco24I GRGCYC 1 cut(s) 999
Eco31I GGTCTC 1 cut(s) 566
Eco47I GGWCC 1 cut(s) 620
Eco52I CGGCCG 1 cut(s) 785
Eco53kI GAGCTC 1 cut(s) 997
Eco57I CTGAAG 3 cut(s) 777, 786, 806
EcoICRI GAGCTC 1 cut(s) 997
EcoNI CCTNNNNNAGG 2 cut(s) 191, 373
EcoRII CCWGG 2 cut(s) 119, 179
EcoT14I CCWWGG 1 cut(s) 808
EcoT38I GRGCYC 1 cut(s) 999
ErhI CCWWGG 1 cut(s) 808
Esp3I CGTCTC 1 cut(s) 969
FaeI CATG 3 cut(s) 541, 880, 1021
FaqI GGGAC 3 cut(s) 152, 271, 511
FatI CATG 3 cut(s) 537, 876, 1017
FauNDI CATATG 1 cut(s) 502
FbaI TGATCA 1 cut(s) 703
Fnu4HI GCNGC 6 cut(s) 63, 84, 157, 297, 522, 543
FokI GGATG 3 cut(s) 859, 874, 995
FriOI GRGCYC 1 cut(s) 999
Fsp4HI GCNGC 6 cut(s) 63, 84, 157, 297, 522, 543
FspBI CTAG 5 cut(s) 426, 494, 722, 809, 1042
GluI GCNGC 6 cut(s) 63, 84, 157, 297, 522, 543
HaeIII GGCC 7 cut(s) 5, 236, 296, 342, 465, 787, 863
HapII CCGG 2 cut(s) 466, 896
HgaI GACGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 3 cut(s) 541, 880, 1021
HinfI GANTC 5 cut(s) 140, 277, 813, 869, 953
HpaII CCGG 2 cut(s) 466, 896
HphI GGTGA 4 cut(s) 6, 121, 169, 1027
Hpy166II GTNNAC 1 cut(s) 912
Hpy188I TCNGA 1 cut(s) 21
Hpy188III TCNNGA 4 cut(s) 348, 707, 755, 920
Hpy8I GTNNAC 1 cut(s) 912
HpyAV CCTTC 2 cut(s) 332, 369
HpyCH4III ACNGT 3 cut(s) 97, 749, 1039
HpyCH4IV ACGT 1 cut(s) 660
HpyCH4V TGCA 7 cut(s) 38, 50, 77, 83, 524, 569, 912
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 305, 527, 803, 901
HpyF3I CTNAG 3 cut(s) 18, 587, 906
HpySE526I ACGT 1 cut(s) 660
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 3 cut(s) 541, 880, 1021
Ksp22I TGATCA 1 cut(s) 703
Kzo9I GATC 3 cut(s) 365, 583, 703
LguI GCTCTTC 1 cut(s) 374
LmnI GCTCC 1 cut(s) 164
Lsp1109I GCAGC 5 cut(s) 49, 95, 168, 508, 554
LweI GCATC 2 cut(s) 231, 970
MabI ACCWGGT 1 cut(s) 179
MaeI CTAG 5 cut(s) 426, 494, 722, 809, 1042
MaeII ACGT 1 cut(s) 660
MaeIII GTNAC 6 cut(s) 100, 175, 208, 571, 593, 685
MalI GATC 3 cut(s) 367, 585, 705
MboI GATC 3 cut(s) 365, 583, 703
MboII GAAGA 5 cut(s) 391, 456, 527, 721, 770
MflI RGATCY 1 cut(s) 365
MhlI GDGCHC 2 cut(s) 914, 999
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 264, 459, 671
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 134, 807, 863
MmeI TCCRAC 1 cut(s) 658
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 69
MslI CAYNNNNRTG 2 cut(s) 107, 438
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 466, 896
MspR9I CCNGG 3 cut(s) 121, 181, 467
MvaI CCWGG 2 cut(s) 121, 181
MwoI GCNNNNNNNGC 5 cut(s) 83, 305, 527, 803, 901
NciI CCSGG 1 cut(s) 467
NdeI CATATG 1 cut(s) 502
NdeII GATC 3 cut(s) 365, 583, 703
NlaIII CATG 3 cut(s) 541, 880, 1021
NlaIV GGNNCC 2 cut(s) 185, 367
NmeAIII GCCGAG 2 cut(s) 159, 763
NmuCI GTSAC 2 cut(s) 100, 175
OliI CACNNNNGTG 1 cut(s) 107
PciSI GCTCTTC 1 cut(s) 374
PfeI GAWTC 2 cut(s) 277, 953
PflFI GACNNNGTC 1 cut(s) 883
PkrI GCNGC 6 cut(s) 64, 85, 158, 298, 523, 544
PleI GAGTC 3 cut(s) 134, 807, 863
PpsI GAGTC 3 cut(s) 134, 807, 863
Psp124BI GAGCTC 1 cut(s) 999
Psp1406I AACGTT 1 cut(s) 660
Psp6I CCWGG 2 cut(s) 119, 179
PspGI CCWGG 2 cut(s) 119, 179
PspN4I GGNNCC 2 cut(s) 185, 367
PspPI GGNCC 1 cut(s) 620
PsuI RGATCY 1 cut(s) 365
PsyI GACNNNGTC 1 cut(s) 883
RseI CAYNNNNRTG 2 cut(s) 107, 438
SacI GAGCTC 1 cut(s) 999
SapI GCTCTTC 1 cut(s) 374
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 6 cut(s) 63, 84, 157, 297, 522, 543
Sau3AI GATC 3 cut(s) 365, 583, 703
Sau96I GGNCC 1 cut(s) 620
SchI GAGTC 3 cut(s) 134, 807, 863
ScrFI CCNGG 3 cut(s) 121, 181, 467
SduI GDGCHC 2 cut(s) 914, 999
SexAI ACCWGGT 1 cut(s) 179
SfaNI GCATC 2 cut(s) 231, 970
SfcI CTRYAG 1 cut(s) 546
SinI GGWCC 1 cut(s) 620
SmiMI CAYNNNNRTG 2 cut(s) 107, 438
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
Sse9I AATT 3 cut(s) 264, 459, 671
SsiI CCGC 2 cut(s) 206, 297
SspMI CTAG 5 cut(s) 426, 494, 722, 809, 1042
SstI GAGCTC 1 cut(s) 999
StyD4I CCNGG 3 cut(s) 119, 179, 465
StyI CCWWGG 1 cut(s) 808
TaaI ACNGT 3 cut(s) 97, 749, 1039
TaiI ACGT 1 cut(s) 663
TaqI TCGA 4 cut(s) 169, 225, 457, 942
TasI AATT 3 cut(s) 264, 459, 671
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 2 cut(s) 277, 953
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 4 cut(s) 391, 799, 914, 997
TseFI GTSAC 2 cut(s) 100, 175
TseI GCWGC 5 cut(s) 62, 83, 156, 521, 542
Tsp45I GTSAC 2 cut(s) 100, 175
TspDTI ATGAA 2 cut(s) 290, 341
TspRI CASTG 4 cut(s) 391, 799, 914, 997
Tth111I GACNNNGTC 1 cut(s) 883
VneI GTGCAC 1 cut(s) 910
VpaK11BI GGWCC 1 cut(s) 620
XagI CCTNNNNNAGG 2 cut(s) 191, 373
XmaJI CCTAGG 1 cut(s) 808
XspI CTAG 5 cut(s) 426, 494, 722, 809, 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.