Rorug05G0554700

Peptidase of plants and bacteria

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001463
Physical Location & Seq
Reverse (-)
74295999 .. 74297243
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0554700.1

Sequence Viewer

Length: 1245 bp
ATGGAAGACGTTGATGAGGATTCAGTTTCAGAGCTCCCAAGCGAAATCATATGCTCAGAAATACTCCCAAGGCTACCAGCCAAGTCCCTGATGCGCTTCAGATGTGTTAGTAAGCCATGGTCTTCTCTCTTCCGCAACCCTTCCTTTGTCACAGCCCACCGAAACTACCAGCAGTGCCCTAACAAGCAGATGACTAGAGTCCTCGTCGCTGTTTGGGACAAATCCGGAGCCCACCAACACTTGCTGTCAGTCCAAATCAGCCACCAAACAAGTACTAGTAATGATCTATCACAGGCTGTAGCCACCGACCTTCTAAAACTGTCCGCACCAACTCGAAGTGATCTCTACCAAGTACAGCGCACCAATGGCTTGATCTGCCTTCACTTTTGTTATATGTCACCGCAGAACGGTGAGCGTGAGCCTGACCGCGCCATTCGCGTATTTAATCCTAGCACTCGAGATTCCATTACTATTCCCCAGCCTTCCCCTCCTACCGGACGATGTGTCGAGGTTTCACACCACTTAGGGTTCTGTCCTGTTACGAACGAATATAAACTTCTCCAAGTCCAAAGGTTTTGGGACGAAAGGCCTAGACGTACGATAGTGTTCAATATTATCACGCTGGCCATGGGTTCCAGCAGCAGTGTTACTTCTTCATGGAGGCTCTTAGAGGTAGACAATGATGATGCTCTCCCGCTAAAGCTTCTTAATTGCCACTTTGAGAACGGAAGTGTGTGCGTCCGGGGGGCCTTACATTGGGTGGATAGGGTTCGACTATCGCGCTCTGATGAACTGGAGACGACTATTTTAGTAGCTTTCGATGTAAGAGACGAGAGATTCAGATCTATCATTCTACCTAGTGATAACATTACCGACCACTACATTGGTCACATTATTGAACTAAATGGATGTGTAGGTGTGGCATATGTCCGTACGCAAACGATGGAGTTGAGGATTTTGAAGGACTACAAAAATCTAGAATGGGTTAAGGAGACTACAAGCTTTCCCGTTCATTGGAGAGAGATAAAAAAGGTTTTTTTAGGCAGCGTCCATACCGGAGGCGATGTTATTCTCTTAAAAGTGAGCAACATGGCCAATGGCGTACGAGTTCATCTTTACAATACGAAAACTAAAAATTTCGAAAGTCGCGAAATTGTTTTGCCTGAATCGATAATCTTAGAGGATAGTACCGATGCATTGCGAGCATTTATTAGTTATGAGTCATGCATTGTGCCCTTAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSP

Protein Analysis

414

Amino Acids

47.19

Weight (kDa)

6.92

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 80 - 387 1.2e-31 F-box associated beta propeller domain
FBA_1 PF07734 87 - 291 6e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 675
AccII CGCG 4 cut(s) 429, 438, 781, 1149
AccIII TCCGGA 1 cut(s) 224
AciI CCGC 5 cut(s) 133, 324, 401, 427, 695
AcoI YGGCCR 2 cut(s) 624, 1092
AcsI RAATTY 1 cut(s) 1135
AcuI CTGAAG 1 cut(s) 82
AfaI GTAC 6 cut(s) 274, 354, 598, 934, 1104, 1189
AfiI CCNNNNNNNGG 4 cut(s) 407, 494, 756, 1014
AgsI TTSAA 3 cut(s) 610, 899, 961
AhdI GACNNNNNGTC 1 cut(s) 503
AhlI ACTAGT 1 cut(s) 275
AluBI AGCT 4 cut(s) 34, 703, 815, 1002
AluI AGCT 4 cut(s) 34, 703, 815, 1002
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 3 cut(s) 791, 822, 986
Ama87I CYCGRG 1 cut(s) 456
Aor13HI TCCGGA 1 cut(s) 224
AoxI GGCC 4 cut(s) 587, 624, 747, 1092
ApeKI GCWGC 2 cut(s) 639, 1044
ApoI RAATTY 1 cut(s) 1135
AspLEI GCGC 4 cut(s) 96, 360, 431, 783
AspS9I GGNCC 1 cut(s) 747
AsuC2I CCSGG 1 cut(s) 743
AsuHPI GGTGA 2 cut(s) 390, 422
AsuII TTCGAA 1 cut(s) 1140
AvaI CYCGRG 1 cut(s) 456
BaeGI GKGCMC 2 cut(s) 179, 1236
BalI TGGCCA 2 cut(s) 626, 1094
BanII GRGCYC 2 cut(s) 36, 232
BbsI GAAGAC 2 cut(s) 12, 114
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 651, 1056
BccI CCATC 1 cut(s) 937
BcnI CCSGG 1 cut(s) 743
BcoDI GTCTC 3 cut(s) 791, 822, 986
BcuI ACTAGT 1 cut(s) 275
BfaI CTAG 6 cut(s) 195, 276, 450, 591, 858, 977
BfmI CTRYAG 1 cut(s) 297
BglII AGATCT 1 cut(s) 842
BisI GCNGC 2 cut(s) 640, 1045
BlsI GCNGC 2 cut(s) 641, 1046
BmcAI AGTACT 1 cut(s) 274
Bme1390I CCNGG 1 cut(s) 743
BmeRI GACNNNNNGTC 1 cut(s) 503
BmeT110I CYCGRG 1 cut(s) 456
BmgT120I GGNCC 1 cut(s) 747
BmiI GGNNCC 3 cut(s) 229, 634, 748
BmrFI CCNGG 1 cut(s) 743
BmsI GCATC 3 cut(s) 81, 676, 1183
BoxI GACNNNNGTC 1 cut(s) 197
BpiI GAAGAC 2 cut(s) 12, 114
BpmI CTGGAG 1 cut(s) 815
Bpu14I TTCGAA 1 cut(s) 1140
BpuMI CCSGG 1 cut(s) 743
Bsa29I ATCGAT 1 cut(s) 1169
BsaBI GATNNNNATC 1 cut(s) 841
BsaJI CCNNGG 4 cut(s) 68, 116, 627, 742
BsaWI WCCGGW 3 cut(s) 224, 494, 1055
BsaXI ACNNNNNCTCC 2 cut(s) 1050, 1080
Bsc4I CCNNNNNNNGG 4 cut(s) 407, 494, 756, 1014
Bse1I ACTGG 1 cut(s) 798
Bse3DI GCAATG 1 cut(s) 1196
Bse8I GATNNNNATC 1 cut(s) 841
BseAI TCCGGA 1 cut(s) 224
BseCI ATCGAT 1 cut(s) 1169
BseDI CCNNGG 4 cut(s) 68, 116, 627, 742
BseGI GGATG 1 cut(s) 914
BseJI GATNNNNATC 1 cut(s) 841
BseLI CCNNNNNNNGG 4 cut(s) 407, 494, 756, 1014
BseMI GCAATG 1 cut(s) 1196
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 1 cut(s) 798
BseSI GKGCMC 2 cut(s) 179, 1236
BseXI GCAGC 2 cut(s) 651, 1056
BseYI CCCAGC 1 cut(s) 477
Bsh1236I CGCG 4 cut(s) 429, 438, 781, 1149
BshFI GGCC 4 cut(s) 589, 626, 749, 1094
BshVI ATCGAT 1 cut(s) 1169
BsiHKAI GWGCWC 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 456
BsiSI CCGG 4 cut(s) 225, 495, 742, 1056
BsiWI CGTACG 3 cut(s) 596, 932, 1102
BslFI GGGAC 3 cut(s) 70, 230, 593
BslI CCNNNNNNNGG 4 cut(s) 407, 494, 756, 1014
BsmAI GTCTC 3 cut(s) 791, 822, 986
BsmBI CGTCTC 2 cut(s) 791, 822
BsmFI GGGAC 3 cut(s) 70, 230, 593
BsnI GGCC 4 cut(s) 589, 626, 749, 1094
BsoBI CYCGRG 1 cut(s) 456
Bsp119I TTCGAA 1 cut(s) 1140
Bsp1286I GDGCHC 4 cut(s) 36, 179, 232, 1236
Bsp13I TCCGGA 1 cut(s) 224
Bsp143I GATC 4 cut(s) 283, 340, 372, 842
Bsp19I CCATGG 2 cut(s) 116, 627
Bsp68I TCGCGA 1 cut(s) 1149
BspACI CCGC 5 cut(s) 133, 324, 401, 427, 695
BspANI GGCC 4 cut(s) 589, 626, 749, 1094
BspCNI CTCAG 1 cut(s) 68
BspDI ATCGAT 1 cut(s) 1169
BspEI TCCGGA 1 cut(s) 224
BspFNI CGCG 4 cut(s) 429, 438, 781, 1149
BspLI GGNNCC 3 cut(s) 229, 634, 748
BspT104I TTCGAA 1 cut(s) 1140
BsrDI GCAATG 1 cut(s) 1196
BsrI ACTGG 1 cut(s) 798
BssECI CCNNGG 4 cut(s) 68, 116, 627, 742
BssMI GATC 4 cut(s) 283, 340, 372, 842
BssT1I CCWWGG 3 cut(s) 68, 116, 627
Bst4CI ACNGT 2 cut(s) 321, 410
Bst6I CTCTTC 1 cut(s) 134
BstBI TTCGAA 1 cut(s) 1140
BstC8I GCNNGC 2 cut(s) 624, 1203
BstDEI CTNAG 4 cut(s) 55, 523, 667, 1177
BstDSI CCRYGG 2 cut(s) 116, 627
BstF5I GGATG 1 cut(s) 914
BstFNI CGCG 4 cut(s) 429, 438, 781, 1149
BstHHI GCGC 4 cut(s) 96, 360, 431, 783
BstKTI GATC 4 cut(s) 286, 343, 375, 845
BstMAI GTCTC 3 cut(s) 791, 822, 986
BstMBI GATC 4 cut(s) 283, 340, 372, 842
BstMWI GCNNNNNNNGC 4 cut(s) 366, 375, 435, 1202
BstPAI GACNNNNGTC 1 cut(s) 197
BstSCI CCNGG 1 cut(s) 741
BstSFI CTRYAG 1 cut(s) 297
BstSLI GKGCMC 2 cut(s) 179, 1236
BstUI CGCG 4 cut(s) 429, 438, 781, 1149
BstV1I GCAGC 2 cut(s) 651, 1056
BstV2I GAAGAC 2 cut(s) 12, 114
BstX2I RGATCY 1 cut(s) 842
BstXI CCANNNNNNTGG 1 cut(s) 884
BstYI RGATCY 1 cut(s) 842
Bsu15I ATCGAT 1 cut(s) 1169
BsuRI GGCC 4 cut(s) 589, 626, 749, 1094
BsuTUI ATCGAT 1 cut(s) 1169
BtgI CCRYGG 2 cut(s) 116, 627
BtgZI GCGATG 1 cut(s) 1077
BtsCI GGATG 1 cut(s) 914
BtsI GCAGTG 2 cut(s) 179, 649
BtsIMutI CAGTG 2 cut(s) 179, 649
BtuMI TCGCGA 1 cut(s) 1149
Cac8I GCNNGC 2 cut(s) 624, 1203
CfoI GCGC 4 cut(s) 96, 360, 431, 783
Cfr13I GGNCC 1 cut(s) 747
ClaI ATCGAT 1 cut(s) 1169
CseI GACGC 2 cut(s) 727, 1036
Csp6I GTAC 6 cut(s) 273, 353, 597, 933, 1103, 1188
CviAII CATG 5 cut(s) 117, 628, 657, 1090, 1224
CviQI GTAC 6 cut(s) 273, 353, 597, 933, 1103, 1188
DdeI CTNAG 4 cut(s) 55, 523, 667, 1177
DpnI GATC 4 cut(s) 285, 342, 374, 844
DpnII GATC 4 cut(s) 283, 340, 372, 842
DriI GACNNNNNGTC 1 cut(s) 503
EaeI YGGCCR 2 cut(s) 624, 1092
Eam1104I CTCTTC 1 cut(s) 134
Eam1105I GACNNNNNGTC 1 cut(s) 503
EarI CTCTTC 1 cut(s) 134
Ecl136II GAGCTC 1 cut(s) 34
Eco130I CCWWGG 3 cut(s) 68, 116, 627
Eco147I AGGCCT 1 cut(s) 589
Eco24I GRGCYC 2 cut(s) 36, 232
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 456
EcoICRI GAGCTC 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 747
EcoT14I CCWWGG 3 cut(s) 68, 116, 627
EcoT22I ATGCAT 2 cut(s) 1198, 1229
EcoT38I GRGCYC 2 cut(s) 36, 232
ErhI CCWWGG 3 cut(s) 68, 116, 627
Esp3I CGTCTC 2 cut(s) 791, 822
FaeI CATG 5 cut(s) 120, 631, 660, 1093, 1227
FaqI GGGAC 3 cut(s) 70, 230, 593
FatI CATG 5 cut(s) 116, 627, 656, 1089, 1223
FauI CCCGC 1 cut(s) 702
FauNDI CATATG 2 cut(s) 50, 925
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 2 cut(s) 640, 1045
FokI GGATG 1 cut(s) 921
FriOI GRGCYC 2 cut(s) 36, 232
Fsp4HI GCNGC 2 cut(s) 640, 1045
FspBI CTAG 6 cut(s) 195, 276, 450, 591, 858, 977
GlaI GCGC 4 cut(s) 95, 359, 430, 782
GluI GCNGC 2 cut(s) 640, 1045
GsaI CCCAGC 1 cut(s) 481
GsuI CTGGAG 1 cut(s) 815
HaeIII GGCC 4 cut(s) 589, 626, 749, 1094
HapII CCGG 4 cut(s) 225, 495, 742, 1056
HgaI GACGC 2 cut(s) 727, 1036
HhaI GCGC 4 cut(s) 96, 360, 431, 783
Hin1II CATG 5 cut(s) 120, 631, 660, 1093, 1227
Hin6I GCGC 4 cut(s) 94, 358, 429, 781
HinP1I GCGC 4 cut(s) 94, 358, 429, 781
HindIII AAGCTT 2 cut(s) 701, 1000
HinfI GANTC 6 cut(s) 20, 198, 461, 837, 1166, 1220
HpaII CCGG 4 cut(s) 225, 495, 742, 1056
HphI GGTGA 2 cut(s) 390, 422
Hpy166II GTNNAC 1 cut(s) 676
Hpy188I TCNGA 5 cut(s) 31, 58, 101, 787, 842
Hpy188III TCNNGA 4 cut(s) 225, 458, 977, 1148
Hpy8I GTNNAC 1 cut(s) 676
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 5 cut(s) 150, 320, 389, 492, 955
HpyCH4III ACNGT 2 cut(s) 321, 410
HpyCH4IV ACGT 2 cut(s) 9, 595
HpyCH4V TGCA 2 cut(s) 1196, 1227
HpyF10VI GCNNNNNNNGC 4 cut(s) 366, 375, 435, 1202
HpyF3I CTNAG 4 cut(s) 55, 523, 667, 1177
HpySE526I ACGT 2 cut(s) 9, 595
Hsp92II CATG 5 cut(s) 120, 631, 660, 1093, 1227
HspAI GCGC 4 cut(s) 94, 358, 429, 781
Kpn2I TCCGGA 1 cut(s) 224
Kzo9I GATC 4 cut(s) 283, 340, 372, 842
LmnI GCTCC 2 cut(s) 39, 227
Lsp1109I GCAGC 2 cut(s) 651, 1056
LweI GCATC 3 cut(s) 81, 676, 1183
MaeI CTAG 6 cut(s) 195, 276, 450, 591, 858, 977
MaeII ACGT 2 cut(s) 9, 595
MaeIII GTNAC 5 cut(s) 148, 396, 538, 646, 887
MalI GATC 4 cut(s) 285, 342, 374, 844
MboI GATC 4 cut(s) 283, 340, 372, 842
MboII GAAGA 4 cut(s) 17, 114, 121, 645
MflI RGATCY 1 cut(s) 842
MhlI GDGCHC 4 cut(s) 36, 179, 232, 1236
MlsI TGGCCA 2 cut(s) 626, 1094
MluCI AATT 3 cut(s) 709, 1135, 1152
MluNI TGGCCA 2 cut(s) 626, 1094
MlyI GAGTC 2 cut(s) 207, 1229
MnlI CCTC 9 cut(s) 10, 212, 498, 502, 654, 664, 945, 1052, 1174
Mox20I TGGCCA 2 cut(s) 626, 1094
Mph1103I ATGCAT 2 cut(s) 1198, 1229
MroI TCCGGA 1 cut(s) 224
MscI TGGCCA 2 cut(s) 626, 1094
MseI TTAA 5 cut(s) 444, 708, 987, 1076, 1238
Msp20I TGGCCA 2 cut(s) 626, 1094
MspI CCGG 4 cut(s) 225, 495, 742, 1056
MspR9I CCNGG 1 cut(s) 743
MvnI CGCG 4 cut(s) 429, 438, 781, 1149
MwoI GCNNNNNNNGC 4 cut(s) 366, 375, 435, 1202
NciI CCSGG 1 cut(s) 743
NcoI CCATGG 2 cut(s) 116, 627
NdeI CATATG 2 cut(s) 50, 925
NdeII GATC 4 cut(s) 283, 340, 372, 842
NlaIII CATG 5 cut(s) 120, 631, 660, 1093, 1227
NlaIV GGNNCC 3 cut(s) 229, 634, 748
NmuCI GTSAC 3 cut(s) 148, 396, 887
NruI TCGCGA 1 cut(s) 1149
NsiI ATGCAT 2 cut(s) 1198, 1229
NspV TTCGAA 1 cut(s) 1140
PaeR7I CTCGAG 1 cut(s) 456
PceI AGGCCT 1 cut(s) 589
PcsI WCGNNNNNNNCGW 1 cut(s) 1146
PfeI GAWTC 4 cut(s) 20, 461, 837, 1166
Pfl23II CGTACG 3 cut(s) 596, 932, 1102
PkrI GCNGC 2 cut(s) 641, 1046
PleI GAGTC 2 cut(s) 206, 1228
PpsI GAGTC 2 cut(s) 206, 1228
PshAI GACNNNNGTC 1 cut(s) 197
Psp124BI GAGCTC 1 cut(s) 36
PspFI CCCAGC 1 cut(s) 477
PspLI CGTACG 3 cut(s) 596, 932, 1102
PspN4I GGNNCC 3 cut(s) 229, 634, 748
PspPI GGNCC 1 cut(s) 747
PsuI RGATCY 1 cut(s) 842
RruI TCGCGA 1 cut(s) 1149
RsaI GTAC 6 cut(s) 274, 354, 598, 934, 1104, 1189
RsaNI GTAC 6 cut(s) 273, 353, 597, 933, 1103, 1188
SacI GAGCTC 1 cut(s) 36
SaqAI TTAA 5 cut(s) 444, 708, 987, 1076, 1238
SatI GCNGC 2 cut(s) 640, 1045
Sau3AI GATC 4 cut(s) 283, 340, 372, 842
Sau96I GGNCC 1 cut(s) 747
ScaI AGTACT 1 cut(s) 274
SchI GAGTC 2 cut(s) 207, 1229
ScrFI CCNGG 1 cut(s) 743
SduI GDGCHC 4 cut(s) 36, 179, 232, 1236
SfaNI GCATC 3 cut(s) 81, 676, 1183
SfcI CTRYAG 1 cut(s) 297
Sfr274I CTCGAG 1 cut(s) 456
SfuI TTCGAA 1 cut(s) 1140
SlaI CTCGAG 1 cut(s) 456
SmlI CTYRAG 1 cut(s) 456
SmoI CTYRAG 1 cut(s) 456
SpeI ACTAGT 1 cut(s) 275
Sse9I AATT 3 cut(s) 709, 1135, 1152
SseBI AGGCCT 1 cut(s) 589
SsiI CCGC 5 cut(s) 133, 324, 401, 427, 695
SspI AATATT 1 cut(s) 613
SspMI CTAG 6 cut(s) 195, 276, 450, 591, 858, 977
SstI GAGCTC 1 cut(s) 36
StuI AGGCCT 1 cut(s) 589
StyD4I CCNGG 1 cut(s) 741
StyI CCWWGG 3 cut(s) 68, 116, 627
TaaI ACNGT 2 cut(s) 321, 410
TaiI ACGT 2 cut(s) 12, 598
TaqI TCGA 7 cut(s) 334, 457, 507, 772, 819, 1140, 1169
TasI AATT 3 cut(s) 709, 1135, 1152
TatI WGTACW 2 cut(s) 272, 352
TfiI GAWTC 4 cut(s) 20, 461, 837, 1166
Tru1I TTAA 5 cut(s) 444, 708, 987, 1076, 1238
Tru9I TTAA 5 cut(s) 444, 708, 987, 1076, 1238
TscAI CASTG 2 cut(s) 179, 649
TseFI GTSAC 3 cut(s) 148, 396, 887
TseI GCWGC 2 cut(s) 639, 1044
Tsp45I GTSAC 3 cut(s) 148, 396, 887
TspDTI ATGAA 4 cut(s) 645, 804, 1001, 1100
TspGWI ACGGA 2 cut(s) 741, 920
TspRI CASTG 2 cut(s) 179, 649
XapI RAATTY 1 cut(s) 1135
XbaI TCTAGA 1 cut(s) 976
XhoI CTCGAG 1 cut(s) 456
XmiI GTMKAC 1 cut(s) 675
XspI CTAG 6 cut(s) 195, 276, 450, 591, 858, 977
ZrmI AGTACT 1 cut(s) 274
Zsp2I ATGCAT 2 cut(s) 1198, 1229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.