Rorug06G0195500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003705
Physical Location & Seq
Forward (+)
31605772 .. 31607978
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0195500.1

Sequence Viewer

Length: 558 bp
ATGTCAGTGGCATCTTCCTTCCCCTGCATCAAAATCTCAACTTGCTCATCATCCCCATCTTGTTCATATTCTTCTTCTTCCTCTAGTAATTCTCTTATGTTGTCTTCTTCTTCAACTAAGCCCTGTTATGTAAAGACCACCATTAGGAGCTCCCAAACTGAAGGGCCTATAAGAAGACCTGCTGCTCCTTCAGTTAGAGAACCCTCTCCACCTACTCTCAAACCCCCAACACCTCCCTCTAAGTCTCCTTCGCCGCCGCAGCCTCAGCCGGCGGCTCCGGTTTCCGGGCTGATGATGGTGGAGGACAAGAATGTGGTGACACTCGAGTTTCAGAGGCAGAAGGCTAAGGAGATTCAGGACTACTTCAAGCAGAAGAAGCTTGAGGAAGCCAGTCAGGGTCCTTTCTTTGGCTTTGTTGGCAAGAATGAGATTAGCAATGGAAGATGGGCGATGTTTGGTTTTGCTGTTGGAATGCTAACAGAGTATGCAACTGGCTCAGACTTTGTTGATCAAGTAAAGATCCTTCTCTCCAATTTTGGGATAGTAGATCTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

19.97

Weight (kDa)

8.9

Isoelectric Point (pI)

68.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 187
AciI CCGC 3 cut(s) 254, 257, 272
AclWI GGATC 1 cut(s) 514
AcuI CTGAAG 2 cut(s) 174, 180
AfiI CCNNNNNNNGG 4 cut(s) 144, 284, 407, 537
AgsI TTSAA 2 cut(s) 114, 367
AluBI AGCT 2 cut(s) 150, 379
AluI AGCT 2 cut(s) 150, 379
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 1 cut(s) 249
AlwI GGATC 1 cut(s) 514
Ama87I CYCGRG 1 cut(s) 323
AoxI GGCC 1 cut(s) 164
ApeKI GCWGC 2 cut(s) 182, 259
AspS9I GGNCC 2 cut(s) 164, 398
AsuC2I CCSGG 1 cut(s) 286
AsuHPI GGTGA 1 cut(s) 328
AvaI CYCGRG 1 cut(s) 323
AvaII GGWCC 1 cut(s) 398
BanII GRGCYC 1 cut(s) 152
BarI GAAGNNNNNNTAC 2 cut(s) 507, 539
BbsI GAAGAC 2 cut(s) 96, 181
Bbv12I GWGCWC 1 cut(s) 152
BbvCI CCTCAGC 1 cut(s) 264
BbvI GCAGC 2 cut(s) 169, 271
BccI CCATC 3 cut(s) 64, 289, 438
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 249
BfaI CTAG 1 cut(s) 84
BfuAI ACCTGC 1 cut(s) 187
BglII AGATCT 1 cut(s) 547
BisI GCNGC 5 cut(s) 183, 254, 257, 260, 273
BlsI GCNGC 5 cut(s) 184, 255, 258, 261, 274
Bme1390I CCNGG 1 cut(s) 286
Bme18I GGWCC 1 cut(s) 398
BmeT110I CYCGRG 1 cut(s) 323
BmgT120I GGNCC 2 cut(s) 164, 398
BmiI GGNNCC 2 cut(s) 276, 399
BmrFI CCNGG 1 cut(s) 286
BmsI GCATC 2 cut(s) 20, 36
BpiI GAAGAC 2 cut(s) 96, 181
Bpu10I CCTNAGC 2 cut(s) 264, 345
BpuEI CTTGAG 1 cut(s) 401
BpuMI CCSGG 1 cut(s) 286
BsaWI WCCGGW 1 cut(s) 277
Bsc4I CCNNNNNNNGG 4 cut(s) 144, 284, 407, 537
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 2 cut(s) 390, 496
Bse3DI GCAATG 1 cut(s) 442
BseGI GGATG 1 cut(s) 50
BseLI CCNNNNNNNGG 4 cut(s) 144, 284, 407, 537
BseMI GCAATG 1 cut(s) 442
BseMII CTCAG 2 cut(s) 278, 510
BseNI ACTGG 2 cut(s) 390, 496
BseXI GCAGC 2 cut(s) 169, 271
BshFI GGCC 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 152
BsiHKCI CYCGRG 1 cut(s) 323
BsiSI CCGG 3 cut(s) 269, 278, 285
BslI CCNNNNNNNGG 4 cut(s) 144, 284, 407, 537
BsmAI GTCTC 1 cut(s) 249
BsmI GAATGC 1 cut(s) 477
BsnI GGCC 1 cut(s) 166
BsoBI CYCGRG 1 cut(s) 323
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 3 cut(s) 508, 519, 547
BspACI CCGC 3 cut(s) 254, 257, 272
BspANI GGCC 1 cut(s) 166
BspCNI CTCAG 2 cut(s) 277, 509
BspLI GGNNCC 2 cut(s) 276, 399
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 1 cut(s) 514
BsrDI GCAATG 1 cut(s) 442
BsrFI RCCGGY 1 cut(s) 268
BsrI ACTGG 2 cut(s) 390, 496
BssAI RCCGGY 1 cut(s) 268
BssMI GATC 3 cut(s) 508, 519, 547
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 5 cut(s) 117, 240, 264, 345, 496
BstF5I GGATG 1 cut(s) 50
BstKTI GATC 3 cut(s) 511, 522, 550
BstMAI GTCTC 1 cut(s) 249
BstMBI GATC 3 cut(s) 508, 519, 547
BstMWI GCNNNNNNNGC 4 cut(s) 259, 265, 376, 417
BstSCI CCNGG 1 cut(s) 284
BstV1I GCAGC 2 cut(s) 169, 271
BstV2I GAAGAC 2 cut(s) 96, 181
BstX2I RGATCY 2 cut(s) 519, 547
BstYI RGATCY 2 cut(s) 519, 547
BsuRI GGCC 1 cut(s) 166
BtgZI GCGATG 1 cut(s) 464
BtsCI GGATG 1 cut(s) 50
BtsIMutI CAGTG 1 cut(s) 12
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 1 cut(s) 270
Cfr10I RCCGGY 1 cut(s) 268
Cfr13I GGNCC 2 cut(s) 164, 398
DdeI CTNAG 5 cut(s) 117, 240, 264, 345, 496
DpnI GATC 3 cut(s) 510, 521, 549
DpnII GATC 3 cut(s) 508, 519, 547
Ecl136II GAGCTC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 152
Eco47I GGWCC 1 cut(s) 398
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 2 cut(s) 174, 180
Eco88I CYCGRG 1 cut(s) 323
EcoICRI GAGCTC 1 cut(s) 150
EcoO109I RGGNCCY 2 cut(s) 164, 398
EcoT38I GRGCYC 1 cut(s) 152
FaiI YATR 5 cut(s) 67, 98, 129, 170, 486
FbaI TGATCA 1 cut(s) 508
Fnu4HI GCNGC 5 cut(s) 183, 254, 257, 260, 273
FokI GGATG 1 cut(s) 37
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 5 cut(s) 183, 254, 257, 260, 273
FspBI CTAG 1 cut(s) 84
GluI GCNGC 5 cut(s) 183, 254, 257, 260, 273
HaeIII GGCC 1 cut(s) 166
HapII CCGG 3 cut(s) 269, 278, 285
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 1 cut(s) 352
HpaII CCGG 3 cut(s) 269, 278, 285
HphI GGTGA 1 cut(s) 328
Hpy188I TCNGA 2 cut(s) 333, 499
Hpy188III TCNNGA 2 cut(s) 356, 551
HpyAV CCTTC 6 cut(s) 28, 155, 198, 258, 334, 533
HpyCH4V TGCA 2 cut(s) 27, 488
HpyF10VI GCNNNNNNNGC 4 cut(s) 259, 265, 376, 417
HpyF3I CTNAG 5 cut(s) 117, 240, 264, 345, 496
KroI GCCGGC 1 cut(s) 268
KroNI GCCGGC 1 cut(s) 270
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 3 cut(s) 508, 519, 547
LmnI GCTCC 4 cut(s) 147, 155, 190, 280
Lsp1109I GCAGC 2 cut(s) 169, 271
LweI GCATC 2 cut(s) 20, 36
MaeI CTAG 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 316
MalI GATC 3 cut(s) 510, 521, 549
MboI GATC 3 cut(s) 508, 519, 547
MflI RGATCY 2 cut(s) 519, 547
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 2 cut(s) 88, 532
MmeI TCCRAC 1 cut(s) 448
MnlI CCTC 8 cut(s) 91, 214, 243, 247, 273, 295, 327, 376
MroNI GCCGGC 1 cut(s) 268
MspI CCGG 3 cut(s) 269, 278, 285
MspR9I CCNGG 1 cut(s) 286
Mva1269I GAATGC 1 cut(s) 477
MwoI GCNNNNNNNGC 4 cut(s) 259, 265, 376, 417
NaeI GCCGGC 1 cut(s) 270
NciI CCSGG 1 cut(s) 286
NdeII GATC 3 cut(s) 508, 519, 547
NgoMIV GCCGGC 1 cut(s) 268
NlaIV GGNNCC 2 cut(s) 276, 399
NmuCI GTSAC 1 cut(s) 316
PaeR7I CTCGAG 1 cut(s) 323
PctI GAATGC 1 cut(s) 477
PdiI GCCGGC 1 cut(s) 270
PfeI GAWTC 1 cut(s) 352
PkrI GCNGC 5 cut(s) 184, 255, 258, 261, 274
PpuMI RGGWCCY 1 cut(s) 398
Psp124BI GAGCTC 1 cut(s) 152
Psp5II RGGWCCY 1 cut(s) 398
PspN4I GGNNCC 2 cut(s) 276, 399
PspPI GGNCC 2 cut(s) 164, 398
PspPPI RGGWCCY 1 cut(s) 398
PspXI VCTCGAGB 1 cut(s) 323
PsuI RGATCY 2 cut(s) 519, 547
SacI GAGCTC 1 cut(s) 152
SatI GCNGC 5 cut(s) 183, 254, 257, 260, 273
Sau3AI GATC 3 cut(s) 508, 519, 547
Sau96I GGNCC 2 cut(s) 164, 398
ScrFI CCNGG 1 cut(s) 286
SduI GDGCHC 1 cut(s) 152
SetI ASST 5 cut(s) 152, 181, 214, 235, 381
SfaNI GCATC 2 cut(s) 20, 36
Sfr274I CTCGAG 1 cut(s) 323
SinI GGWCC 1 cut(s) 398
SlaI CTCGAG 1 cut(s) 323
SmlI CTYRAG 2 cut(s) 323, 380
SmoI CTYRAG 2 cut(s) 323, 380
Sse9I AATT 2 cut(s) 88, 532
SsiI CCGC 3 cut(s) 254, 257, 272
SspMI CTAG 1 cut(s) 84
SstI GAGCTC 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 284
TaqI TCGA 1 cut(s) 324
TasI AATT 2 cut(s) 88, 532
TauI GCSGC 3 cut(s) 256, 259, 275
TfiI GAWTC 1 cut(s) 352
TscAI CASTG 1 cut(s) 12
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 2 cut(s) 182, 259
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 1 cut(s) 54
TspRI CASTG 1 cut(s) 12
VpaK11BI GGWCC 1 cut(s) 398
XhoI CTCGAG 1 cut(s) 323
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.