Rorug06G0498000

cell fate commitment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002310
Physical Location & Seq
Forward (+)
61422257 .. 61424492
2236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0498000.1

Sequence Viewer

Length: 1116 bp
ATGGAGGGAGTAGCAGTGAAAGAAGAAATTATAGCTTGCACAGTTGGTTCATCGTCTTCATCTTCTTCAAGCGTGTCACCGCGGCCGATGGAGGGGTTACACGAGTTGGGTCCGCCACCATTTCTCACAAAGACGTTTGAAATGGTGGAGGACTCTTCTACAGACGCCATTGTTTCCTGGAGCAGAGCTCGCAATAGCTTCATCGTTTGGGACTCTCATCAGTTCTCCTCAATTCTCCTTCCTCGCTACTTCAAGCACAATAATTTCTCGAGCTTCATTCGCCAGCTTAATACATATGGTTTCAGAAAGGTTGATCCGGATAGATGGGAATTTGCTAATGAAGGGTTTCTTGGAGGGCAGAGGCATTTGTTGAAGACTATCAAGAGGAGGAGGCATGTGTCGCAGAGTACGCATGAAGATGGAGGAGGAGCTTGTGTTGAATTGGGACAGTACGGGCTGGAGACTGAGCTTGAAAGACTGAAAAGGGACAGAAATGTGTTGATGACTGAGATAGTGAGATTAAGGCAGCAACAACAGAATTCGAAAGAATTAGTCATTGCGATGGAGGATCGGTTGCAGACGACTGAGAAGAAACAGCAACAGATTATGAATTTCCTTGCAAAAGCACTCAAGAACCCATGTTTTATCCAAAACTTTGCTCAGAAGAGAGAGTTAGTTGGTGTTGGTGTAGGTCGAAAACGGAGACTAAGTGCTAGCCCCAGTGTGGAGAATTTGCAAGAGGTTCCTACAGCCCATGTTTTGGACATGGCAGCAAGTCAAGATCAGGGGGAGTTGTTAACCATGGAGTCAGACTTTGACTCACTTTTCTCAGCTGCTGCTTTGGACAATGAATCAAGCAGTGATATCAACGGCTCTAATCTGACTTCAATACCCACAAGTGGTGGTGGCAACTTGGGCACTGTGAATGAGACAATTTGGGACCACCTGTGGAGTGATGAGCTCATTGGTGGAAATCCAGAGGAAGAGGTTGTGGTTGGGAATGACTCTGAAATCGATGTGGGGGTGGAGGATCTGGTTGCTGATCCTTCAGAATGGGGTGAGGACTTGCAAGACCTTGTTGATCAAATGGATTTTCTGATCAACCCCAATCCTTGA

Protein Analysis

371

Amino Acids

41.37

Weight (kDa)

4.77

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 41 - 130 1.2e-28 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 760
AccII CGCG 1 cut(s) 82
AccIII TCCGGA 1 cut(s) 316
AciI CCGC 3 cut(s) 80, 82, 113
AclWI GGATC 4 cut(s) 308, 576, 1037, 1038
AcoI YGGCCR 1 cut(s) 83
AcsI RAATTY 4 cut(s) 329, 538, 610, 730
AcuI CTGAAG 1 cut(s) 1032
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 2 cut(s) 409, 452
AfiI CCNNNNNNNGG 4 cut(s) 92, 724, 760, 899
AgsI TTSAA 7 cut(s) 69, 140, 253, 373, 440, 473, 888
AjnI CCWGG 1 cut(s) 176
AjuI GAANNNNNNNTTGG 2 cut(s) 333, 365
AluBI AGCT 9 cut(s) 35, 188, 198, 273, 286, 431, 469, 833, 961
AluI AGCT 9 cut(s) 35, 188, 198, 273, 286, 431, 469, 833, 961
Alw21I GWGCWC 2 cut(s) 190, 963
Alw26I GTCTC 3 cut(s) 455, 697, 923
AlwI GGATC 4 cut(s) 308, 576, 1037, 1038
AlwNI CAGNNNCTG 1 cut(s) 836
Ama87I CYCGRG 1 cut(s) 268
Aor13HI TCCGGA 1 cut(s) 316
AoxI GGCC 1 cut(s) 83
ApeKI GCWGC 4 cut(s) 526, 770, 833, 836
ApoI RAATTY 4 cut(s) 329, 538, 610, 730
Asp700I GAANNNNTTC 1 cut(s) 345
AspS9I GGNCC 2 cut(s) 110, 940
AsuHPI GGTGA 2 cut(s) 69, 1070
AsuII TTCGAA 1 cut(s) 542
AsuNHI GCTAGC 1 cut(s) 713
AvaI CYCGRG 1 cut(s) 268
AvaII GGWCC 2 cut(s) 110, 940
BaeGI GKGCMC 1 cut(s) 920
BanII GRGCYC 2 cut(s) 190, 963
BauI CACGAG 1 cut(s) 101
BbsI GAAGAC 2 cut(s) 48, 380
Bbv12I GWGCWC 2 cut(s) 190, 963
BbvI GCAGC 4 cut(s) 538, 782, 820, 823
BccI CCATC 4 cut(s) 82, 318, 413, 556
BceAI ACGGC 1 cut(s) 886
BciT130I CCWGG 1 cut(s) 178
BclI TGATCA 2 cut(s) 1081, 1098
BcoDI GTCTC 3 cut(s) 455, 697, 923
BfaI CTAG 1 cut(s) 714
BfmI CTRYAG 2 cut(s) 159, 747
BisI GCNGC 5 cut(s) 83, 527, 771, 834, 837
BlsI GCNGC 5 cut(s) 84, 528, 772, 835, 838
Bme1390I CCNGG 1 cut(s) 178
Bme18I GGWCC 2 cut(s) 110, 940
BmeT110I CYCGRG 1 cut(s) 268
BmgT120I GGNCC 2 cut(s) 110, 940
BmiI GGNNCC 3 cut(s) 111, 744, 941
BmrFI CCNGG 1 cut(s) 178
BmrI ACTGGG 1 cut(s) 714
BmtI GCTAGC 1 cut(s) 717
BmuI ACTGGG 1 cut(s) 714
BpiI GAAGAC 2 cut(s) 48, 380
BplI GAGNNNNNCTC 2 cut(s) 172, 204
BpmI CTGGAG 2 cut(s) 199, 479
Bpu14I TTCGAA 1 cut(s) 542
BpuEI CTTGAG 1 cut(s) 614
Bsa29I ATCGAT 1 cut(s) 1014
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 2 cut(s) 80, 801
BsaWI WCCGGW 1 cut(s) 316
BsaXI ACNNNNNCTCC 7 cut(s) 30, 420, 450, 557, 587, 694, 724
Bsc4I CCNNNNNNNGG 4 cut(s) 92, 724, 760, 899
Bse1I ACTGG 1 cut(s) 720
Bse3DI GCAATG 1 cut(s) 555
BseAI TCCGGA 1 cut(s) 316
BseBI CCWGG 1 cut(s) 178
BseCI ATCGAT 1 cut(s) 1014
BseDI CCNNGG 2 cut(s) 80, 801
BseLI CCNNNNNNNGG 4 cut(s) 92, 724, 760, 899
BseMI GCAATG 1 cut(s) 555
BseMII CTCAG 5 cut(s) 456, 498, 576, 674, 843
BseNI ACTGG 1 cut(s) 720
BseRI GAGGAG 5 cut(s) 217, 400, 403, 438, 441
BseSI GKGCMC 1 cut(s) 920
BseX3I CGGCCG 1 cut(s) 83
BseXI GCAGC 4 cut(s) 538, 782, 820, 823
Bsh1236I CGCG 1 cut(s) 82
Bsh1285I CGRYCG 1 cut(s) 86
BshFI GGCC 1 cut(s) 85
BshVI ATCGAT 1 cut(s) 1014
BsiEI CGRYCG 1 cut(s) 86
BsiHKAI GWGCWC 2 cut(s) 190, 963
BsiHKCI CYCGRG 1 cut(s) 268
BsiSI CCGG 1 cut(s) 317
BslFI GGGAC 4 cut(s) 224, 459, 500, 953
BslI CCNNNNNNNGG 4 cut(s) 92, 724, 760, 899
BsmAI GTCTC 3 cut(s) 455, 697, 923
BsmFI GGGAC 4 cut(s) 224, 459, 500, 953
BsnI GGCC 1 cut(s) 85
BsoBI CYCGRG 1 cut(s) 268
Bsp119I TTCGAA 1 cut(s) 542
Bsp1286I GDGCHC 3 cut(s) 190, 920, 963
Bsp13I TCCGGA 1 cut(s) 316
Bsp143I GATC 7 cut(s) 313, 568, 781, 1030, 1042, 1081, 1098
Bsp19I CCATGG 1 cut(s) 801
BspACI CCGC 3 cut(s) 80, 82, 113
BspANI GGCC 1 cut(s) 85
BspCNI CTCAG 5 cut(s) 457, 499, 577, 673, 842
BspDI ATCGAT 1 cut(s) 1014
BspEI TCCGGA 1 cut(s) 316
BspFNI CGCG 1 cut(s) 82
BspLI GGNNCC 3 cut(s) 111, 744, 941
BspOI GCTAGC 1 cut(s) 717
BspPI GGATC 4 cut(s) 308, 576, 1037, 1038
BspT104I TTCGAA 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 555
BsrI ACTGG 1 cut(s) 720
BssECI CCNNGG 2 cut(s) 80, 801
BssMI GATC 7 cut(s) 313, 568, 781, 1030, 1042, 1081, 1098
BssNI GRCGYC 1 cut(s) 165
BssSI CACGAG 1 cut(s) 101
BssT1I CCWWGG 1 cut(s) 801
Bst2BI CACGAG 1 cut(s) 101
Bst2UI CCWGG 1 cut(s) 178
Bst4CI ACNGT 3 cut(s) 43, 450, 922
Bst6I CTCTTC 3 cut(s) 160, 659, 978
BstACI GRCGYC 1 cut(s) 165
BstBI TTCGAA 1 cut(s) 542
BstC8I GCNNGC 4 cut(s) 37, 190, 284, 715
BstDEI CTNAG 6 cut(s) 465, 507, 585, 660, 707, 829
BstDSI CCRYGG 2 cut(s) 80, 801
BstFNI CGCG 1 cut(s) 82
BstKTI GATC 7 cut(s) 316, 571, 784, 1033, 1045, 1084, 1101
BstMAI GTCTC 3 cut(s) 455, 697, 923
BstMBI GATC 7 cut(s) 313, 568, 781, 1030, 1042, 1081, 1098
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 5 cut(s) 189, 279, 400, 409, 915
BstNI CCWGG 1 cut(s) 178
BstNSI RCATGY 1 cut(s) 398
BstSCI CCNGG 1 cut(s) 176
BstSFI CTRYAG 2 cut(s) 159, 747
BstSLI GKGCMC 1 cut(s) 920
BstUI CGCG 1 cut(s) 82
BstV1I GCAGC 4 cut(s) 538, 782, 820, 823
BstV2I GAAGAC 2 cut(s) 48, 380
BstX2I RGATCY 1 cut(s) 1030
BstYI RGATCY 1 cut(s) 1030
BstZI CGGCCG 1 cut(s) 83
Bsu15I ATCGAT 1 cut(s) 1014
BsuRI GGCC 1 cut(s) 85
BsuTUI ATCGAT 1 cut(s) 1014
BtgI CCRYGG 2 cut(s) 80, 801
BtgZI GCGATG 1 cut(s) 575
BtsI GCAGTG 2 cut(s) 21, 865
BtsIMutI CAGTG 4 cut(s) 21, 727, 865, 918
Cac8I GCNNGC 4 cut(s) 37, 190, 284, 715
CaiI CAGNNNCTG 1 cut(s) 836
Cfr13I GGNCC 2 cut(s) 110, 940
Cfr42I CCGCGG 1 cut(s) 83
ClaI ATCGAT 1 cut(s) 1014
CseI GACGC 1 cut(s) 173
Csp6I GTAC 2 cut(s) 408, 451
CviAII CATG 6 cut(s) 395, 413, 639, 755, 766, 802
CviQI GTAC 2 cut(s) 408, 451
DdeI CTNAG 6 cut(s) 465, 507, 585, 660, 707, 829
DpnI GATC 7 cut(s) 315, 570, 783, 1032, 1044, 1083, 1100
DpnII GATC 7 cut(s) 313, 568, 781, 1030, 1042, 1081, 1098
EaeI YGGCCR 1 cut(s) 83
EagI CGGCCG 1 cut(s) 83
Eam1104I CTCTTC 3 cut(s) 160, 659, 978
EarI CTCTTC 3 cut(s) 160, 659, 978
EciI GGCGGA 1 cut(s) 102
Ecl136II GAGCTC 2 cut(s) 188, 961
EclXI CGGCCG 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 801
Eco24I GRGCYC 2 cut(s) 190, 963
Eco32I GATATC 1 cut(s) 865
Eco47I GGWCC 2 cut(s) 110, 940
Eco52I CGGCCG 1 cut(s) 83
Eco53kI GAGCTC 2 cut(s) 188, 961
Eco57I CTGAAG 1 cut(s) 1032
Eco88I CYCGRG 1 cut(s) 268
EcoICRI GAGCTC 2 cut(s) 188, 961
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 1 cut(s) 176
EcoRV GATATC 1 cut(s) 865
EcoT14I CCWWGG 1 cut(s) 801
EcoT38I GRGCYC 2 cut(s) 190, 963
ErhI CCWWGG 1 cut(s) 801
FaeI CATG 6 cut(s) 398, 416, 642, 758, 769, 805
FalI AAGNNNNNCTT 2 cut(s) 333, 365
FaqI GGGAC 4 cut(s) 224, 459, 500, 953
FatI CATG 6 cut(s) 394, 412, 638, 754, 765, 801
FauNDI CATATG 1 cut(s) 295
FbaI TGATCA 2 cut(s) 1081, 1098
Fnu4HI GCNGC 5 cut(s) 83, 527, 771, 834, 837
FriOI GRGCYC 2 cut(s) 190, 963
Fsp4HI GCNGC 5 cut(s) 83, 527, 771, 834, 837
FspBI CTAG 1 cut(s) 714
GluI GCNGC 5 cut(s) 83, 527, 771, 834, 837
GsuI CTGGAG 2 cut(s) 199, 479
HaeIII GGCC 1 cut(s) 85
HapII CCGG 1 cut(s) 317
HgaI GACGC 1 cut(s) 173
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 6 cut(s) 398, 416, 642, 758, 769, 805
HincII GTYRAC 1 cut(s) 798
HindII GTYRAC 1 cut(s) 798
HinfI GANTC 6 cut(s) 152, 212, 806, 818, 851, 1004
HpaI GTTAAC 1 cut(s) 798
HpaII CCGG 1 cut(s) 317
HphI GGTGA 2 cut(s) 69, 1070
Hpy166II GTNNAC 1 cut(s) 798
Hpy188I TCNGA 7 cut(s) 305, 663, 811, 882, 1009, 1051, 1098
Hpy188III TCNNGA 6 cut(s) 268, 317, 382, 631, 779, 977
Hpy8I GTNNAC 1 cut(s) 798
HpyAV CCTTC 3 cut(s) 248, 335, 1056
HpyCH4III ACNGT 3 cut(s) 43, 450, 922
HpyCH4IV ACGT 1 cut(s) 134
HpyCH4V TGCA 5 cut(s) 39, 577, 620, 736, 1069
HpyF10VI GCNNNNNNNGC 5 cut(s) 189, 279, 400, 409, 915
HpyF3I CTNAG 6 cut(s) 465, 507, 585, 660, 707, 829
HpySE526I ACGT 1 cut(s) 134
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 6 cut(s) 398, 416, 642, 758, 769, 805
Kpn2I TCCGGA 1 cut(s) 316
Ksp22I TGATCA 2 cut(s) 1081, 1098
KspAI GTTAAC 1 cut(s) 798
KspI CCGCGG 1 cut(s) 83
Kzo9I GATC 7 cut(s) 313, 568, 781, 1030, 1042, 1081, 1098
LmnI GCTCC 2 cut(s) 180, 428
Lsp1109I GCAGC 4 cut(s) 538, 782, 820, 823
MaeI CTAG 1 cut(s) 714
MaeII ACGT 1 cut(s) 134
MaeIII GTNAC 2 cut(s) 75, 96
MalI GATC 7 cut(s) 315, 570, 783, 1032, 1044, 1083, 1100
MboI GATC 7 cut(s) 313, 568, 781, 1030, 1042, 1081, 1098
MflI RGATCY 1 cut(s) 1030
MhlI GDGCHC 3 cut(s) 190, 920, 963
MlyI GAGTC 5 cut(s) 146, 206, 812, 815, 998
MroI TCCGGA 1 cut(s) 316
MroXI GAANNNNTTC 1 cut(s) 345
MseI TTAA 3 cut(s) 288, 521, 797
MslI CAYNNNNRTG 2 cut(s) 417, 560
MspA1I CMGCKG 2 cut(s) 82, 833
MspI CCGG 1 cut(s) 317
MspR9I CCNGG 1 cut(s) 178
MvaI CCWGG 1 cut(s) 178
MvnI CGCG 1 cut(s) 82
MwoI GCNNNNNNNGC 5 cut(s) 189, 279, 400, 409, 915
NcoI CCATGG 1 cut(s) 801
NdeI CATATG 1 cut(s) 295
NdeII GATC 7 cut(s) 313, 568, 781, 1030, 1042, 1081, 1098
NheI GCTAGC 1 cut(s) 713
NlaIII CATG 6 cut(s) 398, 416, 642, 758, 769, 805
NlaIV GGNNCC 3 cut(s) 111, 744, 941
NmuCI GTSAC 1 cut(s) 75
NspI RCATGY 1 cut(s) 398
NspV TTCGAA 1 cut(s) 542
PaeR7I CTCGAG 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 1 cut(s) 851
PflMI CCANNNNNTGG 1 cut(s) 760
PfoI TCCNGGA 1 cut(s) 176
PkrI GCNGC 5 cut(s) 84, 528, 772, 835, 838
PleI GAGTC 5 cut(s) 146, 206, 812, 814, 998
PpsI GAGTC 5 cut(s) 146, 206, 812, 814, 998
Psp124BI GAGCTC 2 cut(s) 190, 963
Psp6I CCWGG 1 cut(s) 176
PspGI CCWGG 1 cut(s) 176
PspN4I GGNNCC 3 cut(s) 111, 744, 941
PspPI GGNCC 2 cut(s) 110, 940
PstNI CAGNNNCTG 1 cut(s) 836
PsuI RGATCY 1 cut(s) 1030
PvuII CAGCTG 1 cut(s) 833
RsaI GTAC 2 cut(s) 409, 452
RsaNI GTAC 2 cut(s) 408, 451
RseI CAYNNNNRTG 2 cut(s) 417, 560
SacI GAGCTC 2 cut(s) 190, 963
SacII CCGCGG 1 cut(s) 83
SaqAI TTAA 3 cut(s) 288, 521, 797
SatI GCNGC 5 cut(s) 83, 527, 771, 834, 837
Sau3AI GATC 7 cut(s) 313, 568, 781, 1030, 1042, 1081, 1098
Sau96I GGNCC 2 cut(s) 110, 940
SchI GAGTC 5 cut(s) 146, 206, 812, 815, 998
ScrFI CCNGG 1 cut(s) 178
SduI GDGCHC 3 cut(s) 190, 920, 963
SfcI CTRYAG 2 cut(s) 159, 747
Sfr274I CTCGAG 1 cut(s) 268
Sfr303I CCGCGG 1 cut(s) 83
SfuI TTCGAA 1 cut(s) 542
SgrBI CCGCGG 1 cut(s) 83
SinI GGWCC 2 cut(s) 110, 940
SlaI CTCGAG 1 cut(s) 268
SmiMI CAYNNNNRTG 2 cut(s) 417, 560
SmlI CTYRAG 2 cut(s) 268, 629
SmoI CTYRAG 2 cut(s) 268, 629
SsiI CCGC 3 cut(s) 80, 82, 113
SspMI CTAG 1 cut(s) 714
SstI GAGCTC 2 cut(s) 190, 963
StyD4I CCNGG 1 cut(s) 176
StyI CCWWGG 1 cut(s) 801
TaaI ACNGT 3 cut(s) 43, 450, 922
TaiI ACGT 1 cut(s) 137
TaqI TCGA 4 cut(s) 269, 542, 694, 1014
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 1 cut(s) 851
Tru1I TTAA 3 cut(s) 288, 521, 797
Tru9I TTAA 3 cut(s) 288, 521, 797
TscAI CASTG 4 cut(s) 21, 727, 865, 925
TseFI GTSAC 1 cut(s) 75
TseI GCWGC 4 cut(s) 526, 770, 833, 836
Tsp45I GTSAC 1 cut(s) 75
TspDTI ATGAA 8 cut(s) 39, 48, 190, 265, 354, 429, 623, 864
TspGWI ACGGA 1 cut(s) 715
TspRI CASTG 4 cut(s) 21, 727, 865, 925
Van91I CCANNNNNTGG 1 cut(s) 760
VpaK11BI GGWCC 2 cut(s) 110, 940
XapI RAATTY 4 cut(s) 329, 538, 610, 730
XceI RCATGY 1 cut(s) 398
XhoI CTCGAG 1 cut(s) 268
XmnI GAANNNNTTC 1 cut(s) 345
XspI CTAG 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.