Rh2DG300400

function. Its presence in a non-photosynthetic plant (Epifagus virginiana) and experiments in tobacco indicate that it has an essential function which is probably not related to photosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004273
Physical Location & Seq
Reverse (-)
35742424 .. 35743293
870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG300400.1

Sequence Viewer

Length: 870 bp
ATGAAGAAGAAATCATGTAACGAAGGGGGTTCTTATTTGTACAAATGGTACTTCGAACTTGGAATGAGCATGAAGAAATTAACGATATTTCTTTATCTTTTGAGTTGTTCTACCGGATCGGTCGCTCAAGACCTTTGGTCTCTACCCAGACCCGATGAAAAAAAGGGGATTACTTCTTATGGACTCGTTGAGAATGATTCTGATCTAGTTCATGGCCTATTAGAAGTAGAATCATATGGCGCTCCGGTGGGATCCTCCAGGACAGAAAAAGATTGCAGTCAGTTTGATAATGATCGAGTGACATTGCTTCTTCGGCCCGAACCAAGGAATCCCTTAGATATGATGCAAAATGGATCTTGTTCTATTGTTGGTCAGAGATTTTTCTATGAACAATACAAATCGGAGTTTGAAGAAGGGGAAGGGGAAGGAGTCCTCAACCTACAACGGATAGAGGAGGATTTATTCAATCACATAGTTTGGGCTCCTAGAATATGGCGCCCTTGGGGCTTTCTATTTGATTGTATCAAAAGGCCCAATGAATTGGGATTTCCCTATTGGGTCAAATCATTTCAGGGCAAACGAATCATTTATGATGAAAAGGATGGGCTTCAAGAGAATGATTCAGAGTTCTTGCAGAGTCCGTACCAGACACGAGATAGATCTTCCAAAGAACAAGGCTTCTTTCGAATAAGCCAATTCGTTTGGGACCCTGTGGATCTACTTTTTTTCCTATTCAAAGATCCGCCTTTTATCTCTGTGTTTTCACATCGAGAATTCTTTGCAGATGAAGAGATGCCAAAGGGGCTTCTTACTTCCCAAACAGATCTTCCTAAAACTCTATATAAACGCTGGTTTATCAAGAATACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA DUF825

Protein Analysis

289

Amino Acids

33.93

Weight (kDa)

5.24

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 495
AciI CCGC 1 cut(s) 743
AclWI GGATC 6 cut(s) 124, 246, 259, 361, 723, 734
AcsI RAATTY 1 cut(s) 773
AcyI GRCGYC 1 cut(s) 496
AfaI GTAC 3 cut(s) 41, 50, 644
AfiI CCNNNNNNNGG 1 cut(s) 324
AgsI TTSAA 4 cut(s) 410, 466, 611, 736
AhdI GACNNNNNGTC 1 cut(s) 136
AjnI CCWGG 1 cut(s) 257
AjuI GAANNNNNNNTTGG 2 cut(s) 810, 842
Alw26I GTCTC 1 cut(s) 144
AlwI GGATC 6 cut(s) 124, 246, 259, 361, 723, 734
AoxI GGCC 3 cut(s) 214, 314, 530
ApoI RAATTY 1 cut(s) 773
AspLEI GCGC 2 cut(s) 242, 498
AspS9I GGNCC 3 cut(s) 315, 531, 706
AsuII TTCGAA 2 cut(s) 54, 685
AvaII GGWCC 1 cut(s) 706
BamHI GGATCC 1 cut(s) 251
BanI GGYRCC 1 cut(s) 495
BanII GRGCYC 1 cut(s) 484
BauI CACGAG 1 cut(s) 651
BccI CCATC 1 cut(s) 596
BciT130I CCWGG 1 cut(s) 259
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 2 cut(s) 206, 486
BfoI RGCGCY 2 cut(s) 243, 499
BglI GCCNNNNNGGC 2 cut(s) 504, 802
BglII AGATCT 2 cut(s) 659, 823
Bme1390I CCNGG 1 cut(s) 259
Bme18I GGWCC 1 cut(s) 706
BmeRI GACNNNNNGTC 1 cut(s) 136
BmgT120I GGNCC 3 cut(s) 315, 531, 706
BmiI GGNNCC 5 cut(s) 253, 483, 497, 707, 708
BmrFI CCNGG 1 cut(s) 259
BmsI GCATC 2 cut(s) 333, 783
BpmI CTGGAG 1 cut(s) 241
Bpu14I TTCGAA 2 cut(s) 54, 685
BpuEI CTTGAG 1 cut(s) 111
BsaAI YACGTR 1 cut(s) 867
BsaBI GATNNNNATC 2 cut(s) 201, 291
BsaHI GRCGYC 1 cut(s) 496
BsaI GGTCTC 1 cut(s) 144
BsaJI CCNNGG 2 cut(s) 323, 500
BsaWI WCCGGW 2 cut(s) 113, 244
Bsc4I CCNNNNNNNGG 1 cut(s) 324
Bse3DI GCAATG 1 cut(s) 302
Bse8I GATNNNNATC 2 cut(s) 201, 291
BseBI CCWGG 1 cut(s) 259
BseDI CCNNGG 2 cut(s) 323, 500
BseGI GGATG 1 cut(s) 607
BseJI GATNNNNATC 2 cut(s) 201, 291
BseLI CCNNNNNNNGG 1 cut(s) 324
BseMI GCAATG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 467
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 3 cut(s) 216, 316, 532
BshNI GGYRCC 1 cut(s) 495
BsiEI CGRYCG 1 cut(s) 123
BsiSI CCGG 2 cut(s) 114, 245
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 1 cut(s) 324
BsmAI GTCTC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 719
BsnI GGCC 3 cut(s) 216, 316, 532
Bso31I GGTCTC 1 cut(s) 144
Bsp119I TTCGAA 2 cut(s) 54, 685
Bsp1286I GDGCHC 1 cut(s) 484
Bsp1407I TGTACA 1 cut(s) 39
Bsp143I GATC 9 cut(s) 116, 202, 251, 292, 353, 659, 715, 739, 823
BspACI CCGC 1 cut(s) 743
BspANI GGCC 3 cut(s) 216, 316, 532
BspLI GGNNCC 5 cut(s) 253, 483, 497, 707, 708
BspPI GGATC 6 cut(s) 124, 246, 259, 361, 723, 734
BspT104I TTCGAA 2 cut(s) 54, 685
BspT107I GGYRCC 1 cut(s) 495
BspTNI GGTCTC 1 cut(s) 144
BsrDI GCAATG 1 cut(s) 302
BsrGI TGTACA 1 cut(s) 39
BssECI CCNNGG 2 cut(s) 323, 500
BssMI GATC 9 cut(s) 116, 202, 251, 292, 353, 659, 715, 739, 823
BssNI GRCGYC 1 cut(s) 496
BssSI CACGAG 1 cut(s) 651
BssT1I CCWWGG 2 cut(s) 323, 500
Bst2BI CACGAG 1 cut(s) 651
Bst2UI CCWGG 1 cut(s) 259
Bst6I CTCTTC 1 cut(s) 783
BstACI GRCGYC 1 cut(s) 496
BstAUI TGTACA 1 cut(s) 39
BstBAI YACGTR 1 cut(s) 867
BstBI TTCGAA 2 cut(s) 54, 685
BstDEI CTNAG 1 cut(s) 334
BstF5I GGATG 1 cut(s) 607
BstH2I RGCGCY 2 cut(s) 243, 499
BstHHI GCGC 2 cut(s) 242, 498
BstKTI GATC 9 cut(s) 119, 205, 254, 295, 356, 662, 718, 742, 826
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 9 cut(s) 116, 202, 251, 292, 353, 659, 715, 739, 823
BstMCI CGRYCG 1 cut(s) 123
BstMWI GCNNNNNNNGC 3 cut(s) 313, 504, 802
BstNI CCWGG 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 257
BstSNI TACGTA 1 cut(s) 867
BstX2I RGATCY 6 cut(s) 251, 353, 659, 715, 739, 823
BstXI CCANNNNNNTGG 1 cut(s) 541
BstYI RGATCY 6 cut(s) 251, 353, 659, 715, 739, 823
BsuRI GGCC 3 cut(s) 216, 316, 532
BtsCI GGATG 1 cut(s) 607
CfoI GCGC 2 cut(s) 242, 498
Cfr13I GGNCC 3 cut(s) 315, 531, 706
Csp6I GTAC 3 cut(s) 40, 49, 643
CviAII CATG 3 cut(s) 15, 70, 212
CviJI RGCY 9 cut(s) 216, 316, 482, 507, 532, 607, 678, 693, 805
CviKI_1 RGCY 9 cut(s) 216, 316, 482, 507, 532, 607, 678, 693, 805
CviQI GTAC 3 cut(s) 40, 49, 643
DdeI CTNAG 1 cut(s) 334
DinI GGCGCC 1 cut(s) 497
DpnI GATC 9 cut(s) 118, 204, 253, 294, 355, 661, 717, 741, 825
DpnII GATC 9 cut(s) 116, 202, 251, 292, 353, 659, 715, 739, 823
DriI GACNNNNNGTC 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 783
Eam1105I GACNNNNNGTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 783
EciI GGCGGA 1 cut(s) 732
Eco105I TACGTA 1 cut(s) 867
Eco130I CCWWGG 2 cut(s) 323, 500
Eco24I GRGCYC 1 cut(s) 484
Eco31I GGTCTC 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 706
EcoO109I RGGNCCY 1 cut(s) 706
EcoRI GAATTC 1 cut(s) 773
EcoRII CCWGG 1 cut(s) 257
EcoT14I CCWWGG 2 cut(s) 323, 500
EcoT38I GRGCYC 1 cut(s) 484
EgeI GGCGCC 1 cut(s) 497
EheI GGCGCC 1 cut(s) 497
ErhI CCWWGG 2 cut(s) 323, 500
FaeI CATG 3 cut(s) 18, 73, 215
FaqI GGGAC 1 cut(s) 719
FatI CATG 3 cut(s) 14, 69, 211
FauNDI CATATG 1 cut(s) 235
FokI GGATG 1 cut(s) 614
FriOI GRGCYC 1 cut(s) 484
FspBI CTAG 2 cut(s) 206, 486
GlaI GCGC 2 cut(s) 241, 497
GsuI CTGGAG 1 cut(s) 241
HaeII RGCGCY 2 cut(s) 243, 499
HaeIII GGCC 3 cut(s) 216, 316, 532
HapII CCGG 2 cut(s) 114, 245
HhaI GCGC 2 cut(s) 242, 498
Hin1I GRCGYC 1 cut(s) 496
Hin1II CATG 3 cut(s) 18, 73, 215
Hin6I GCGC 2 cut(s) 240, 496
HinP1I GCGC 2 cut(s) 240, 496
HinfI GANTC 8 cut(s) 183, 197, 230, 328, 429, 582, 620, 637
HpaII CCGG 2 cut(s) 114, 245
Hpy188I TCNGA 4 cut(s) 202, 375, 403, 625
Hpy188III TCNNGA 4 cut(s) 128, 611, 770, 859
HpyAV CCTTC 4 cut(s) 17, 407, 413, 419
HpyCH4IV ACGT 1 cut(s) 866
HpyCH4V TGCA 4 cut(s) 276, 346, 634, 782
HpyF10VI GCNNNNNNNGC 3 cut(s) 313, 504, 802
HpyF3I CTNAG 1 cut(s) 334
HpySE526I ACGT 1 cut(s) 866
Hsp92I GRCGYC 1 cut(s) 496
Hsp92II CATG 3 cut(s) 18, 73, 215
HspAI GCGC 2 cut(s) 240, 496
KasI GGCGCC 1 cut(s) 495
KflI GGGWCCC 1 cut(s) 706
Kzo9I GATC 9 cut(s) 116, 202, 251, 292, 353, 659, 715, 739, 823
LmnI GCTCC 2 cut(s) 247, 487
LpnPI CCDG 9 cut(s) 127, 160, 244, 258, 271, 557, 659, 723, 835
LweI GCATC 2 cut(s) 333, 783
MaeI CTAG 2 cut(s) 206, 486
MaeII ACGT 1 cut(s) 866
MaeIII GTNAC 2 cut(s) 17, 298
MalI GATC 9 cut(s) 118, 204, 253, 294, 355, 661, 717, 741, 825
MboI GATC 9 cut(s) 116, 202, 251, 292, 353, 659, 715, 739, 823
MboII GAAGA 8 cut(s) 16, 19, 85, 302, 422, 654, 800, 818
MflI RGATCY 6 cut(s) 251, 353, 659, 715, 739, 823
MhlI GDGCHC 1 cut(s) 484
MluCI AATT 4 cut(s) 77, 539, 695, 773
Mly113I GGCGCC 1 cut(s) 496
MlyI GAGTC 3 cut(s) 177, 438, 646
MnlI CCTC 4 cut(s) 265, 443, 445, 448
MseI TTAA 1 cut(s) 80
MspI CCGG 2 cut(s) 114, 245
MspR9I CCNGG 1 cut(s) 259
MvaI CCWGG 1 cut(s) 259
MwoI GCNNNNNNNGC 3 cut(s) 313, 504, 802
NarI GGCGCC 1 cut(s) 496
NdeI CATATG 1 cut(s) 235
NdeII GATC 9 cut(s) 116, 202, 251, 292, 353, 659, 715, 739, 823
NlaIII CATG 3 cut(s) 18, 73, 215
NlaIV GGNNCC 5 cut(s) 253, 483, 497, 707, 708
NmuCI GTSAC 1 cut(s) 298
NspV TTCGAA 2 cut(s) 54, 685
PfeI GAWTC 5 cut(s) 197, 230, 328, 582, 620
PfoI TCCNGGA 1 cut(s) 257
PleI GAGTC 3 cut(s) 177, 437, 645
PluTI GGCGCC 1 cut(s) 499
PpsI GAGTC 3 cut(s) 177, 437, 645
Ppu21I YACGTR 1 cut(s) 867
PpuMI RGGWCCY 1 cut(s) 706
Psp5II RGGWCCY 1 cut(s) 706
Psp6I CCWGG 1 cut(s) 257
PspGI CCWGG 1 cut(s) 257
PspN4I GGNNCC 5 cut(s) 253, 483, 497, 707, 708
PspPI GGNCC 3 cut(s) 315, 531, 706
PspPPI RGGWCCY 1 cut(s) 706
PsuI RGATCY 6 cut(s) 251, 353, 659, 715, 739, 823
RsaI GTAC 3 cut(s) 41, 50, 644
RsaNI GTAC 3 cut(s) 40, 49, 643
SaqAI TTAA 1 cut(s) 80
Sau3AI GATC 9 cut(s) 116, 202, 251, 292, 353, 659, 715, 739, 823
Sau96I GGNCC 3 cut(s) 315, 531, 706
SchI GAGTC 3 cut(s) 177, 438, 646
ScrFI CCNGG 1 cut(s) 259
SduI GDGCHC 1 cut(s) 484
SetI ASST 3 cut(s) 135, 441, 869
SfaNI GCATC 2 cut(s) 333, 783
SfoI GGCGCC 1 cut(s) 497
SfuI TTCGAA 2 cut(s) 54, 685
SinI GGWCC 1 cut(s) 706
SmlI CTYRAG 1 cut(s) 126
SmoI CTYRAG 1 cut(s) 126
SnaBI TACGTA 1 cut(s) 867
Sse9I AATT 4 cut(s) 77, 539, 695, 773
SsiI CCGC 1 cut(s) 743
SspDI GGCGCC 1 cut(s) 495
SspMI CTAG 2 cut(s) 206, 486
StyD4I CCNGG 1 cut(s) 257
StyI CCWWGG 2 cut(s) 323, 500
TaiI ACGT 1 cut(s) 869
TaqI TCGA 4 cut(s) 54, 295, 685, 769
TaqII GACCGA 1 cut(s) 109
TasI AATT 4 cut(s) 77, 539, 695, 773
TatI WGTACW 1 cut(s) 39
TfiI GAWTC 5 cut(s) 197, 230, 328, 582, 620
Tru1I TTAA 1 cut(s) 80
Tru9I TTAA 1 cut(s) 80
TseFI GTSAC 1 cut(s) 298
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 8 cut(s) 17, 86, 171, 200, 402, 552, 609, 801
TspGWI ACGGA 2 cut(s) 460, 630
VpaK11BI GGWCC 1 cut(s) 706
XapI RAATTY 1 cut(s) 773
XspI CTAG 2 cut(s) 206, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.