Rw3G020690

function. Its presence in a non-photosynthetic plant (Epifagus virginiana) and experiments in tobacco indicate that it has an essential function which is probably not related to photosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0004273
Physical Location & Seq
Reverse (-)
24498113 .. 24518609
20497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G020690.1

Sequence Viewer

Length: 5823 bp
ATGTTTGCTTTTCGTGAAAAAGTACCAATTGAAGCGGAGGTTTTCTTCAAACAACAAGGAGCTGGGTCAACTATTCAATCAAATGAAATTGAGCATGTTTCCCATCTCTTCTCGAGAAACAAGTGGGCTATGCAAAATTGTGCTCAATTTCATATGTGGCAATTCCGCCAAGATCTCTTCTTTAGTTGGGGGAAGAATCCGCACGAATCGGATTTTTTGAGGAACATATCGAGAGAGAATTGGTTAGACAATGTGTGGTTGGTAAACAAGGATCGGTTTTTTAGCAAGGTACGGAATGTATCGTCAAATATTCAATATGATTCCACAAGATCTAGTTTTGTTCAAGTAACGGATTCTAGCCAATTGAAAGGATCCTCTGATCAATCCAGAGATCGTTTCGATTCCATTAGTAATGAGGATTCAGAATATCACACATTGATCAATCAAAGAGAGATTCAACAACTAAAAGAAAGATCGATTCTTTGGGATCATTCTTTTCTTCAAACGGAACGAACAGAGATAGAATCAGACCGATTCCCTAAATGCCTTTCTGGATATTCCTCAATGTCCCGGCTATTCACGGAACGTGAGAAGCAGATGAATAAGCATCAGCTTCCGGAAGAAATCGAAGAATTTCTTAGGAATCCTACAAGATCCCTTCGTTCTTTTTTCTCTGACAGATGGTCAGAACTTCATCTAGAACAAGATGTTTCTTTTGTCCCTTCCAGGCGATCGGAAAATAAAGAAATAGTTAATATATTCAAGATAATTACGTATTTACAAAATATCGTCTCAATTCATCCTATTTCATCAGATCCGGGATGTGATATGGTTCCAAAGGATGAACTGGACAGTTCCAATAAGATTTCATTCTTGAACAAAAATCTATTTTGTGATTTATTTCATCTATTCCATGACCGGAACAGGGGGGGATACACGTTACACCACGATTTTGAATCAGAAGAGAGATTTCAAGAAATGGCAGATCTATTCACTCTATCAATAACCGAGCCGGATCCGTTATATCATAAGGGATTTGCCTTTTCTATTGATTCCTACGGATTGGATCAAAAACAATTCTTGAATGAGGTATTCAACTCCAGGGATGAATCGAAAAAGAAATCTTTATTGGTTCTACCTCCTATTTTTTATGAAGAGAATGAATCTTTTTATCGAAGGATCAGAAAAAAATGGGTCCGGACCTCCTGCGGGAATGATTTGGAAGATCCAAAACCAAAAATAGTGGTATTTGCTAGCAACAACATAATGGAGGCAGTCAATCAATATAGATTGATCCGAAATCTGATTCAAATCCAATATAGCACCTATGGGTACATAAGAAATGTATTGAATCGATTCTTTTTAATGAATAGATCCGATTGCAACTTCAAATATGGAATTCAAAGGGATCAAATAGAAAATGATACTCTGAATCATAGAACTATAATGAAATATACGATCAACCAACATTTATCAAATTTGAAAAAGAGTCAGAAGAAATGGTTCGATCCTCTTATTTTTATTTCTCGAACCGAGAGATCCATGAATCGGGATCCTAATGCATATAGATACAAATGGTCCAATGGGAGCAAGAAGTTACAGGAACATTTGGAACATTTCATTTCTGAGCAGAAGAGCCATTTTCATTTTCAAGTAGTCGATCGATTACGTATTAATCAATATTCGATTGATTGGTCTGAGGTTATCGACAAAAAAGATTTGTCTAAGTCACTTCCTTTCTTTTTGTCCAAGTTACTTCTTTTTTTGTCCAAGTTTCTTCTCTTTTTGTCTAACTCACTTCCTTTTTTCTTTATGAGTTTCGGGAATATCCCCATTTATAGGTCCGAGATCCACATATATGAATTGAAAGGTCCGAATGATCAACTCTGCAATCAGTTGTTAGAATCAATAGGTCTTCAAATCGTTCATTTGAAAAAATTAAAACCCCTCTTATTAGATGATCATGATACTTCCCAAAAATCAAAATTCTTGATCAATGGAGGAACAATATCACCGTTTTTGTTCAATAAGATTAAGATACCAAAGTGGGTGATTGACTCATTCCATACTAGAAATAATCGCAGGAAATCTTTTGATAACATGGATTCCTATTTCTCAATGATATCCCACGATCAAGACAATTGGCTGAATCCCGTGAAACCATTTCATAGAAGTTCATTGATATCTTCTTTTTATAAAGCAAATCGACTTCGATTCTTGAATAATCCACATCACTTCTGCTTCTATTGTAACAAAGGATTCCCTTTTTTTGTGGAAAAGGCCCGTATCAATAATTATGATTTTACATATGGACAATTCCTCAATAACTTGTTCATTCGCAACAAAATATTTTCTTTGCGCGGCGGTAAAAAAAAATGCTTTTTGGAGAGAGATACTATTTCACCAAATGAGTTACAGGTGTCTAACATATTCATACCTAACGATTTTCCACAAAATGGTGTCGAAAGGTATAACTTGTACAAATCTTTTCATTTTCCGATTCGATCCGATCCATTCATTCGTAGAGCTATTTACTCGATCGCAGACATTTCTGGAACACTTCTAACAGAAGGACAAATAGTCAGTTTTGAAAGAACTTATTGTCAACCCCTTTCATATATGAATCTATCTGATTCAGAAGGGAAGAACTTGCATCAGTATCTCAATTTCAATTCAAATATGGGTTTGATTCACACTCCATGTTCTGAGAAATATTTACCACCCGAAAAGAGGAAAAAGCGGGGTCGTTGTCTAAAGAAATGCTTTGAGAAAGGGCAGATGTATAGAACCTTTCAACGAGATAGTGCTTTTTCAACTCTCTCAAAATGGAATATATTCCAAACATATATGCCATGGTTCCTTACTTCGACAGGATACAAATATCTAAATTTGATATTTTTAGATACTTTTTCAGACCTATTGCCGATACTAAGTAGCAGCCAAAAATTTTTATCCATTTTTCATGATATATCTGATCCATGCATAATATCATGGCGAATTCTTCAGAAAAAATTGTGTCTTCCACAATGGAATATGATAAGTGATATTTCGAGTAAGTGTTTACATAATCTTCTTCTGTCCGAAGAAATGATTCATCGAAATAATGAGTCACCATTGATATCGACACATCTGAGATCGCCAAATGTTCGGGAGTTCCTCTATTCAATCCTTTTCCTTCTTCTTATTGCTGGATATCTCGTTCGTACACATCTTCTCTTTGTTTCTCGAGCCTATAGTGAGTTACAGACAGAGTTCGAAAGGGTCAAATCTTTGATGATTCCATCATACATGATTGAGTTGCGAAAACTTCTGGATAGGTATCCTCCATCTGAACTGAATTCTTTCTGGTTAAAGAATCTCTTTCTAGTTGCTGTGGAACAATTAGGAGATTCTCTAGAAGAAATACGGGGTTCCGCTTCTGGCGGCAACATGCTATGGGGTGGTGGTCCCGCTTATGGGGTCAAATCAATACGTTCTAAGAAGAAATATTTGAATATAAATCTCATCGATATCATCGATTTCATAAGTATCATACCAAATCCCATCAATCGAATCGCTTTTTCGAGAAATACGAGACATCTAAGTCATACAAGTAAAGAGATCTATTCATTGATAAGAAAAAGAAAAAACATTAACGGTGATTGGATTGACGATAAAATAGAATCATGGGTCTCGAACAGTGATTCAATTGGTGATAAAGAAAGAGAATTCTTGGTTCATCTGACTCATAATGATCATTTATCAAAGAATGACTCTGGTTATCAAATGATTGAACAACCGGGAGCAATTTACTTACGATACTTAGTTGACATTCATAAAAAGTATCTAATGAATTATGAGTTCAATACATCCTGTTTAGCAGAAAGGCGGATATTGCTTGCTCATTATCAGACAATCACTTATTCACAAACTGTACATACATATACATTTGCAAGCATAATTAGCTATAATGGTTTTCATTTCCCATCTCATGGAAAACCCTTTTCGCTCCGTTTAGCCCTATCCCTCTCTAGGGGTATTTTAGTGATAGGTTCTATGGGAACTGGACGATCCTATTTGGTCAAATACCTAGCGACAAACTCCTATGTTCCTTTCATTACAGTATTTCTGAACAAGTTCCTGGATAACAAGCCCAAGGGTTTTCTTGTTGATGATAGTGACGATATCGGCCGTGACCTTGATACGGAGCTGGAGCTTCTAACTATGATGAATGCGCTAACTATGGATATGATGCCGGAAATAGACCGATTTTATATCACCCTTCAATTCGAATTAGCAAAATCGAATTACTTATCCCTCGGTTTATTAGTGAGCTATCTCTCTAGGGATTGTGAAAGATGTTCCACTAGAAATATTCTTGTTATTGCTTCGACTCATATTCCCAAAAAAGTGGATCCCGCTCTAATAGCTCCGAATAAATTAAATACATGCATTAAGATACGAAGGCTTCTTATTCCACAACAGCGAAAGCACTTTTTCACTCTTTCATATACTAGGGGATTTCACTTGGAAAAGAAAATGTTCTATACTAATGGATTCGGGTCCATAACCATGGGTTCCAATGTACGAGATCTTGTAGCACTTACCAATGAGGCCCTATCGATTAGTATTACACAGAAGAAATCAATTATAGACACTAATATAATTAGATCTGCTCTTCATAGACAAACTTGGGATTTGCGATCCCAAGTAAGATCGGTTCAGGATCATGGGATCCTTTTCTATCAGATAGGAAGGGCCGTTGCACAAAATGTACTTCTAAGTAATTGCTCCATAGATCCTATATCTATCTATATGAAGAAGAAATCATGTAACGAAAGGGGTTCTTATTTGTACAAATGGTACTTCGAACTTGGAACGAGCATGAAGAAATTAATGATACTTCTTTATCTTTTGAGTTGTTCTGCCGGATCGGTCGCTCAAGACCTTTGGTCTCTACCCGGACCCGATGAAAAAAACGGGATTACTTCTTATGGACTCGTTGAGAATGATTCTGATCTAGTTCATGGCCTATTAGAAGTAGAAGTAGAAGGCGCTCCGGTGGGATCCTCCCGGACAGAAAAAGATTGCAGTCAGTTTGATAATGATCGAGTGACATTGCTTCTTCGGCCCGAACCAAGGAATCCCTTAGATATGATGCAAAATGGATCTTGTTCTATCGTTGATCAGAGATTTTTCTATGAACAATACGAATTGGAGTTTGAAGAAGGGGAAGGAGAAGGAGTCCTCGACCTGCAACGGATAGAGGAGGATTTATTCAATCACATAGTTTGGGCTCCTAGACTATGGCGCCCTTGGGGCTTTCTATTTGATTGTATCGAAAGGCCCAATGAATTGGGATTTTCCTATTGGAATGATTCAGAGTTCTTGCAGAGTCAGTACCAGACACGAGATAGATCTTCCAAACAACAAGGCTTATTTCGAATAAGCCAATTCGTTTGGGACCCTGCGGATCCACTCTTTTTCCTATTCAAAGATCCGCCTTTTATCTCTGTGTTTTCACATCGAGAATTCTTTGCAGATGAAGAGATGCCAAAGGGGCTTCTTACTTCCCAAACAGATCTTCCTACAACTCTATATAAACGCTGGTTTATCAAGAATACGGAAGAAAGGCACTTCGAATTGTTGATTCATCGCCAGAGATGGCTTAGAACCAACAGTTCATTATCTAATGGATTTTTCCGTTCTAATACTCCATCCGAGAGTTATCAGTATTTATCAAAGCTGTTCCTATCTAACGAAACACTATTGGATCAAATGGCAAAGACATTGTTGAGAAAAAGATGGCTTTTCCCGGATGAAATGAAAATTGGATTCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA DUF825

Protein Analysis

1940

Amino Acids

228.15

Weight (kDa)

8.85

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf2 PF05695 1 - 1198 0.0 Ycf2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2196
AasI GACNNNNNNGTC 1 cut(s) 3582
Acc36I ACCTGC 1 cut(s) 5241
AccB1I GGYRCC 1 cut(s) 5289
AccB7I CCANNNNNTGG 2 cut(s) 2456, 3971
AccBSI CCGCTC 1 cut(s) 4400
AccII CGCG 1 cut(s) 2361
AccIII TCCGGA 2 cut(s) 616, 1197
AcoI YGGCCR 1 cut(s) 4168
AcuI CTGAAG 1 cut(s) 2987
AcyI GRCGYC 1 cut(s) 5290
AflIII ACRYGT 1 cut(s) 936
AhdI GACNNNNNGTC 3 cut(s) 682, 2579, 4930
AjnI CCWGG 3 cut(s) 725, 1100, 4119
AjuI GAANNNNNNNTTGG 5 cut(s) 1112, 1144, 5547, 5579, 5793
AloI GAACNNNNNNTCC 2 cut(s) 3183, 3215
AluBI AGCT 9 cut(s) 62, 613, 2528, 3945, 4189, 4195, 4314, 4409, 5725
AluI AGCT 9 cut(s) 62, 613, 2528, 3945, 4189, 4195, 4314, 4409, 5725
Alw21I GWGCWC 1 cut(s) 145
Alw26I GTCTC 4 cut(s) 796, 3568, 3676, 4938
Ama87I CYCGRG 2 cut(s) 112, 3225
Aor13HI TCCGGA 2 cut(s) 616, 1197
AoxI GGCC 7 cut(s) 2280, 4168, 4593, 4737, 5008, 5108, 5324
ApeKI GCWGC 1 cut(s) 2937
AseI ATTAAT 2 cut(s) 1674, 4874
Asp700I GAANNNNTTC 8 cut(s) 633, 1355, 1502, 2834, 3090, 3341, 4115, 4520
AspLEI GCGC 4 cut(s) 2361, 4216, 5036, 5292
AsuC2I CCSGG 6 cut(s) 571, 819, 3780, 4941, 5053, 5795
AsuHPI GGTGA 7 cut(s) 2004, 2062, 2394, 3102, 3650, 3704, 4249
AsuII TTCGAA 5 cut(s) 3255, 4269, 4848, 5422, 5619
AsuNHI GCTAGC 1 cut(s) 1253
AvaI CYCGRG 2 cut(s) 112, 3225
AvaII GGWCC 9 cut(s) 1195, 1200, 1578, 1842, 1871, 3446, 4542, 4943, 5443
BaeI ACNNNNGTAYC 2 cut(s) 2030, 2063
BamHI GGATCC 7 cut(s) 371, 1015, 1552, 4393, 4713, 5045, 5452
BanI GGYRCC 1 cut(s) 5289
BanII GRGCYC 1 cut(s) 5278
BauI CACGAG 1 cut(s) 5388
BbsI GAAGAC 2 cut(s) 1907, 3011
Bbv12I GWGCWC 1 cut(s) 145
BbvI GCAGC 1 cut(s) 2949
BccI CCATC 9 cut(s) 111, 675, 3289, 3334, 3551, 3973, 5637, 5704, 5778
BceAI ACGGC 2 cut(s) 4155, 4724
BciT130I CCWGG 3 cut(s) 727, 1102, 4121
BciVI GTATCC 3 cut(s) 926, 2867, 3330
BclI TGATCA 7 cut(s) 379, 438, 1879, 1960, 1992, 3733, 5164
BcnI CCSGG 6 cut(s) 571, 819, 3780, 4941, 5053, 5795
BcoDI GTCTC 4 cut(s) 796, 3568, 3676, 4938
BfmI CTRYAG 1 cut(s) 3232
BfoI RGCGCY 2 cut(s) 5037, 5293
BfuAI ACCTGC 1 cut(s) 5241
BfuI GTATCC 3 cut(s) 926, 2867, 3330
BglI GCCNNNNNGGC 2 cut(s) 5298, 5539
BglII AGATCT 8 cut(s) 172, 329, 985, 3600, 4570, 4649, 5396, 5560
BisI GCNGC 3 cut(s) 2362, 2938, 3424
BlsI GCNGC 3 cut(s) 2363, 2939, 3425
Bme1390I CCNGG 9 cut(s) 571, 727, 819, 1102, 3780, 4121, 4941, 5053, 5795
Bme18I GGWCC 9 cut(s) 1195, 1200, 1578, 1842, 1871, 3446, 4542, 4943, 5443
BmeRI GACNNNNNGTC 3 cut(s) 682, 2579, 4930
BmeT110I CYCGRG 2 cut(s) 112, 3225
BmrFI CCNGG 9 cut(s) 571, 727, 819, 1102, 3780, 4121, 4941, 5053, 5795
BmsI GCATC 5 cut(s) 616, 2662, 4221, 5127, 5520
BmtI GCTAGC 1 cut(s) 1257
BpiI GAAGAC 2 cut(s) 1907, 3011
BpmI CTGGAG 2 cut(s) 1084, 4211
Bpu14I TTCGAA 5 cut(s) 3255, 4269, 4848, 5422, 5619
BpuEI CTTGAG 1 cut(s) 4905
BpuMI CCSGG 6 cut(s) 571, 819, 3780, 4941, 5053, 5795
Bsa29I ATCGAT 6 cut(s) 476, 1354, 1663, 3507, 3516, 4601
BsaAI YACGTR 2 cut(s) 774, 1670
BsaBI GATNNNNATC 4 cut(s) 1310, 3318, 4995, 5085
BsaHI GRCGYC 1 cut(s) 5290
BsaI GGTCTC 2 cut(s) 3676, 4938
BsaJI CCNNGG 7 cut(s) 1101, 2852, 4134, 4297, 4551, 5117, 5294
BsaWI WCCGGW 4 cut(s) 616, 918, 1197, 5038
BsaXI ACNNNNNCTCC 2 cut(s) 5208, 5238
Bse1I ACTGG 2 cut(s) 852, 4048
Bse3DI GCAATG 1 cut(s) 5096
Bse8I GATNNNNATC 4 cut(s) 1310, 3318, 4995, 5085
BseAI TCCGGA 2 cut(s) 616, 1197
BseBI CCWGG 3 cut(s) 727, 1102, 4121
BseCI ATCGAT 6 cut(s) 476, 1354, 1663, 3507, 3516, 4601
BseDI CCNNGG 7 cut(s) 1101, 2852, 4134, 4297, 4551, 5117, 5294
BseGI GGATG 7 cut(s) 799, 827, 847, 1111, 3848, 5696, 5803
BseJI GATNNNNATC 4 cut(s) 1310, 3318, 4995, 5085
BseMI GCAATG 1 cut(s) 5096
BseMII CTCAG 4 cut(s) 1617, 1689, 2697, 3122
BseNI ACTGG 2 cut(s) 852, 4048
BseRI GAGGAG 1 cut(s) 5261
BseX3I CGGCCG 1 cut(s) 4168
BseXI GCAGC 1 cut(s) 2949
BseYI CCCAGC 1 cut(s) 62
Bsh1236I CGCG 1 cut(s) 2361
Bsh1285I CGRYCG 5 cut(s) 734, 1663, 2541, 4171, 4917
BshFI GGCC 7 cut(s) 2282, 4170, 4595, 4739, 5010, 5110, 5326
BshNI GGYRCC 1 cut(s) 5289
BshVI ATCGAT 6 cut(s) 476, 1354, 1663, 3507, 3516, 4601
BsiEI CGRYCG 5 cut(s) 734, 1663, 2541, 4171, 4917
BsiHKAI GWGCWC 1 cut(s) 145
BsiHKCI CYCGRG 2 cut(s) 112, 3225
BslFI GGGAC 4 cut(s) 553, 704, 3432, 5456
BsmAI GTCTC 4 cut(s) 796, 3568, 3676, 4938
BsmBI CGTCTC 1 cut(s) 796
BsmFI GGGAC 4 cut(s) 553, 704, 3432, 5456
BsmI GAATGC 1 cut(s) 4216
BsnI GGCC 7 cut(s) 2282, 4170, 4595, 4739, 5010, 5110, 5326
Bso31I GGTCTC 2 cut(s) 3676, 4938
BsoBI CYCGRG 2 cut(s) 112, 3225
Bsp119I TTCGAA 5 cut(s) 3255, 4269, 4848, 5422, 5619
Bsp1286I GDGCHC 2 cut(s) 145, 5278
Bsp13I TCCGGA 2 cut(s) 616, 1197
Bsp1407I TGTACA 3 cut(s) 2478, 3913, 4833
Bsp19I CCATGG 2 cut(s) 2852, 4551
BspANI GGCC 7 cut(s) 2282, 4170, 4595, 4739, 5010, 5110, 5326
BspCNI CTCAG 4 cut(s) 1618, 1690, 2698, 3123
BspDI ATCGAT 6 cut(s) 476, 1354, 1663, 3507, 3516, 4601
BspEI TCCGGA 2 cut(s) 616, 1197
BspFNI CGCG 1 cut(s) 2361
BspHI TCATGA 2 cut(s) 1963, 2962
BspMI ACCTGC 1 cut(s) 5241
BspOI GCTAGC 1 cut(s) 1257
BspQI GCTCTTC 2 cut(s) 1628, 4662
BspT104I TTCGAA 5 cut(s) 3255, 4269, 4848, 5422, 5619
BspT107I GGYRCC 1 cut(s) 5289
BspTNI GGTCTC 2 cut(s) 3676, 4938
BsrBI CCGCTC 1 cut(s) 4400
BsrDI GCAATG 1 cut(s) 5096
BsrGI TGTACA 3 cut(s) 2478, 3913, 4833
BsrI ACTGG 2 cut(s) 852, 4048
BssECI CCNNGG 7 cut(s) 1101, 2852, 4134, 4297, 4551, 5117, 5294
BssNI GRCGYC 1 cut(s) 5290
BssSI CACGAG 1 cut(s) 5388
BssT1I CCWWGG 5 cut(s) 2852, 4134, 4551, 5117, 5294
Bst2BI CACGAG 1 cut(s) 5388
Bst2UI CCWGG 3 cut(s) 727, 1102, 4121
Bst4CI ACNGT 7 cut(s) 854, 2016, 3638, 3680, 3913, 4102, 5660
Bst6I CTCTTC 7 cut(s) 113, 182, 957, 1149, 1628, 4662, 5520
BstACI GRCGYC 1 cut(s) 5290
BstAUI TGTACA 3 cut(s) 2478, 3913, 4833
BstBAI YACGTR 2 cut(s) 774, 1670
BstBI TTCGAA 5 cut(s) 3255, 4269, 4848, 5422, 5619
BstC8I GCNNGC 3 cut(s) 1255, 3879, 3934
BstDSI CCRYGG 2 cut(s) 2852, 4551
BstF5I GGATG 7 cut(s) 799, 827, 847, 1111, 3848, 5696, 5803
BstFNI CGCG 1 cut(s) 2361
BstH2I RGCGCY 2 cut(s) 5037, 5293
BstHHI GCGC 4 cut(s) 2361, 4216, 5036, 5292
BstMAI GTCTC 4 cut(s) 796, 3568, 3676, 4938
BstMCI CGRYCG 5 cut(s) 734, 1663, 2541, 4171, 4917
BstMWI GCNNNNNNNGC 6 cut(s) 3874, 3942, 4406, 5107, 5298, 5539
BstNI CCWGG 3 cut(s) 727, 1102, 4121
BstNSI RCATGY 3 cut(s) 98, 3433, 4431
BstSCI CCNGG 9 cut(s) 569, 725, 817, 1100, 3778, 4119, 4939, 5051, 5793
BstSFI CTRYAG 1 cut(s) 3232
BstSNI TACGTA 2 cut(s) 774, 1670
BstUI CGCG 1 cut(s) 2361
BstV1I GCAGC 1 cut(s) 2949
BstV2I GAAGAC 2 cut(s) 1907, 3011
BstXI CCANNNNNNTGG 3 cut(s) 4390, 4552, 5335
BstZI CGGCCG 1 cut(s) 4168
Bsu15I ATCGAT 6 cut(s) 476, 1354, 1663, 3507, 3516, 4601
BsuI GTATCC 3 cut(s) 926, 2867, 3330
BsuRI GGCC 7 cut(s) 2282, 4170, 4595, 4739, 5010, 5110, 5326
BsuTUI ATCGAT 6 cut(s) 476, 1354, 1663, 3507, 3516, 4601
BtgI CCRYGG 2 cut(s) 2852, 4551
BtgZI GCGATG 1 cut(s) 5618
BtsCI GGATG 7 cut(s) 799, 827, 847, 1111, 3848, 5696, 5803
BtsIMutI CAGTG 1 cut(s) 3685
BveI ACCTGC 1 cut(s) 5241
Cac8I GCNNGC 3 cut(s) 1255, 3879, 3934
CciI TCATGA 2 cut(s) 1963, 2962
CfoI GCGC 4 cut(s) 2361, 4216, 5036, 5292
ClaI ATCGAT 6 cut(s) 476, 1354, 1663, 3507, 3516, 4601
CspCI CAANNNNNGTGG 2 cut(s) 1224, 1259
DinI GGCGCC 1 cut(s) 5291
DrdI GACNNNNNNGTC 1 cut(s) 3582
DriI GACNNNNNGTC 3 cut(s) 682, 2579, 4930
DseDI GACNNNNNNGTC 1 cut(s) 3582
EaeI YGGCCR 1 cut(s) 4168
EagI CGGCCG 1 cut(s) 4168
Eam1104I CTCTTC 7 cut(s) 113, 182, 957, 1149, 1628, 4662, 5520
Eam1105I GACNNNNNGTC 3 cut(s) 682, 2579, 4930
EarI CTCTTC 7 cut(s) 113, 182, 957, 1149, 1628, 4662, 5520
EciI GGCGGA 3 cut(s) 155, 3883, 5469
EclXI CGGCCG 1 cut(s) 4168
Eco105I TACGTA 2 cut(s) 774, 1670
Eco130I CCWWGG 5 cut(s) 2852, 4134, 4551, 5117, 5294
Eco24I GRGCYC 1 cut(s) 5278
Eco31I GGTCTC 2 cut(s) 3676, 4938
Eco32I GATATC 6 cut(s) 2124, 2184, 3120, 3194, 3511, 4165
Eco47I GGWCC 9 cut(s) 1195, 1200, 1578, 1842, 1871, 3446, 4542, 4943, 5443
Eco52I CGGCCG 1 cut(s) 4168
Eco57I CTGAAG 1 cut(s) 2987
Eco88I CYCGRG 2 cut(s) 112, 3225
EcoO109I RGGNCCY 2 cut(s) 4594, 5443
EcoRI GAATTC 5 cut(s) 1398, 2997, 3337, 3707, 5510
EcoRII CCWGG 3 cut(s) 725, 1100, 4119
EcoRV GATATC 6 cut(s) 2124, 2184, 3120, 3194, 3511, 4165
EcoT14I CCWWGG 5 cut(s) 2852, 4134, 4551, 5117, 5294
EcoT22I ATGCAT 3 cut(s) 1564, 2984, 4433
EcoT38I GRGCYC 1 cut(s) 5278
EgeI GGCGCC 1 cut(s) 5291
EheI GGCGCC 1 cut(s) 5291
ErhI CCWWGG 5 cut(s) 2852, 4134, 4551, 5117, 5294
Esp3I CGTCTC 1 cut(s) 796
FalI AAGNNNNNCTT 8 cut(s) 621, 653, 2747, 2779, 3344, 3376, 4810, 4842
FaqI GGGAC 4 cut(s) 553, 704, 3432, 5456
FauI CCCGC 4 cut(s) 1202, 2733, 3457, 4405
FauNDI CATATG 2 cut(s) 153, 2308
FbaI TGATCA 7 cut(s) 379, 438, 1879, 1960, 1992, 3733, 5164
Fnu4HI GCNGC 3 cut(s) 2362, 2938, 3424
FokI GGATG 7 cut(s) 786, 834, 854, 1118, 3835, 5683, 5810
FriOI GRGCYC 1 cut(s) 5278
Fsp4HI GCNGC 3 cut(s) 2362, 2938, 3424
GlaI GCGC 4 cut(s) 2360, 4215, 5035, 5291
GluI GCNGC 3 cut(s) 2362, 2938, 3424
GsaI CCCAGC 1 cut(s) 66
GsuI CTGGAG 2 cut(s) 1084, 4211
HaeII RGCGCY 2 cut(s) 5037, 5293
HaeIII GGCC 7 cut(s) 2282, 4170, 4595, 4739, 5010, 5110, 5326
HhaI GCGC 4 cut(s) 2361, 4216, 5036, 5292
Hin1I GRCGYC 1 cut(s) 5290
Hin6I GCGC 4 cut(s) 2359, 4214, 5034, 5290
HinP1I GCGC 4 cut(s) 2359, 4214, 5034, 5290
HincII GTYRAC 3 cut(s) 69, 2606, 3808
HindII GTYRAC 3 cut(s) 69, 2606, 3808
HphI GGTGA 7 cut(s) 2004, 2062, 2394, 3102, 3650, 3704, 4249
Hpy166II GTNNAC 6 cut(s) 69, 265, 2606, 3062, 3206, 3808
Hpy8I GTNNAC 6 cut(s) 69, 265, 2606, 3062, 3206, 3808
HpyCH4III ACNGT 7 cut(s) 854, 2016, 3638, 3680, 3913, 4102, 5660
HpyCH4IV ACGT 5 cut(s) 586, 773, 938, 1669, 3472
HpyF10VI GCNNNNNNNGC 6 cut(s) 3874, 3942, 4406, 5107, 5298, 5539
HpySE526I ACGT 5 cut(s) 586, 773, 938, 1669, 3472
Hsp92I GRCGYC 1 cut(s) 5290
HspAI GCGC 4 cut(s) 2359, 4214, 5034, 5290
KasI GGCGCC 1 cut(s) 5289
KflI GGGWCCC 1 cut(s) 5443
Kpn2I TCCGGA 2 cut(s) 616, 1197
Ksp22I TGATCA 7 cut(s) 379, 438, 1879, 1960, 1992, 3733, 5164
LguI GCTCTTC 2 cut(s) 1628, 4662
Lsp1109I GCAGC 1 cut(s) 2949
LweI GCATC 5 cut(s) 616, 2662, 4221, 5127, 5520
MaeII ACGT 5 cut(s) 586, 773, 938, 1669, 3472
MbiI CCGCTC 1 cut(s) 4400
MfeI CAATTG 4 cut(s) 27, 362, 2140, 3687
MhlI GDGCHC 2 cut(s) 145, 5278
Mly113I GGCGCC 1 cut(s) 5290
MlyI GAGTC 9 cut(s) 1498, 2051, 3116, 3718, 3746, 4366, 4971, 5232, 5383
Mph1103I ATGCAT 3 cut(s) 1564, 2984, 4433
MroI TCCGGA 2 cut(s) 616, 1197
MroXI GAANNNNTTC 8 cut(s) 633, 1355, 1502, 2834, 3090, 3341, 4115, 4520
MslI CAYNNNNRTG 2 cut(s) 1857, 4550
MspR9I CCNGG 9 cut(s) 571, 727, 819, 1102, 3780, 4121, 4941, 5053, 5795
MunI CAATTG 4 cut(s) 27, 362, 2140, 3687
Mva1269I GAATGC 1 cut(s) 4216
MvaI CCWGG 3 cut(s) 727, 1102, 4121
MvnI CGCG 1 cut(s) 2361
MwoI GCNNNNNNNGC 6 cut(s) 3874, 3942, 4406, 5107, 5298, 5539
NarI GGCGCC 1 cut(s) 5290
NciI CCSGG 6 cut(s) 571, 819, 3780, 4941, 5053, 5795
NcoI CCATGG 2 cut(s) 2852, 4551
NdeI CATATG 2 cut(s) 153, 2308
NheI GCTAGC 1 cut(s) 1253
NmuCI GTSAC 5 cut(s) 1728, 3108, 4157, 4172, 5092
NsiI ATGCAT 3 cut(s) 1564, 2984, 4433
NspI RCATGY 3 cut(s) 98, 3433, 4431
NspV TTCGAA 5 cut(s) 3255, 4269, 4848, 5422, 5619
PaeR7I CTCGAG 2 cut(s) 112, 3225
PagI TCATGA 2 cut(s) 1963, 2962
PciSI GCTCTTC 2 cut(s) 1628, 4662
PcsI WCGNNNNNNNCGW 2 cut(s) 3513, 3569
PctI GAATGC 1 cut(s) 4216
PdmI GAANNNNTTC 8 cut(s) 633, 1355, 1502, 2834, 3090, 3341, 4115, 4520
PflMI CCANNNNNTGG 2 cut(s) 2456, 3971
PfoI TCCNGGA 4 cut(s) 817, 4119, 5051, 5793
PkrI GCNGC 3 cut(s) 2363, 2939, 3425
Ple19I CGATCG 3 cut(s) 734, 1663, 2541
PleI GAGTC 9 cut(s) 1497, 2051, 3115, 3718, 3746, 4366, 4971, 5231, 5382
PluTI GGCGCC 1 cut(s) 5293
PpsI GAGTC 9 cut(s) 1497, 2051, 3115, 3718, 3746, 4366, 4971, 5231, 5382
Ppu21I YACGTR 2 cut(s) 774, 1670
PpuMI RGGWCCY 1 cut(s) 5443
PshBI ATTAAT 2 cut(s) 1674, 4874
PsiI TTATAA 1 cut(s) 2196
Psp5II RGGWCCY 1 cut(s) 5443
Psp6I CCWGG 3 cut(s) 725, 1100, 4119
PspFI CCCAGC 1 cut(s) 62
PspGI CCWGG 3 cut(s) 725, 1100, 4119
PspPPI RGGWCCY 1 cut(s) 5443
PsrI GAACNNNNNNTAC 2 cut(s) 4683, 4715
PvuI CGATCG 3 cut(s) 734, 1663, 2541
RseI CAYNNNNRTG 2 cut(s) 1857, 4550
SapI GCTCTTC 2 cut(s) 1628, 4662
SatI GCNGC 3 cut(s) 2362, 2938, 3424
SchI GAGTC 9 cut(s) 1498, 2051, 3116, 3718, 3746, 4366, 4971, 5232, 5383
ScrFI CCNGG 9 cut(s) 571, 727, 819, 1102, 3780, 4121, 4941, 5053, 5795
SduI GDGCHC 2 cut(s) 145, 5278
SfaNI GCATC 5 cut(s) 616, 2662, 4221, 5127, 5520
SfcI CTRYAG 1 cut(s) 3232
SfoI GGCGCC 1 cut(s) 5291
Sfr274I CTCGAG 2 cut(s) 112, 3225
SfuI TTCGAA 5 cut(s) 3255, 4269, 4848, 5422, 5619
SinI GGWCC 9 cut(s) 1195, 1200, 1578, 1842, 1871, 3446, 4542, 4943, 5443
SlaI CTCGAG 2 cut(s) 112, 3225
SmiMI CAYNNNNRTG 2 cut(s) 1857, 4550
SmlI CTYRAG 3 cut(s) 112, 3225, 4920
SmoI CTYRAG 3 cut(s) 112, 3225, 4920
SnaBI TACGTA 2 cut(s) 774, 1670
SspDI GGCGCC 1 cut(s) 5289
SspI AATATT 6 cut(s) 310, 1682, 2349, 2714, 3488, 4354
StyD4I CCNGG 9 cut(s) 569, 725, 817, 1100, 3778, 4119, 4939, 5051, 5793
StyI CCWWGG 5 cut(s) 2852, 4134, 4551, 5117, 5294
TaaI ACNGT 7 cut(s) 854, 2016, 3638, 3680, 3913, 4102, 5660
TaiI ACGT 5 cut(s) 589, 776, 941, 1672, 3475
TaqII GACCGA 3 cut(s) 546, 4260, 4903
TatI WGTACW 4 cut(s) 2478, 3913, 4753, 4833
TauI GCSGC 2 cut(s) 2364, 3426
TscAI CASTG 1 cut(s) 3685
TseFI GTSAC 5 cut(s) 1728, 3108, 4157, 4172, 5092
TseI GCWGC 1 cut(s) 2937
Tsp45I GTSAC 5 cut(s) 1728, 3108, 4157, 4172, 5092
TspRI CASTG 1 cut(s) 3685
Van91I CCANNNNNTGG 2 cut(s) 2456, 3971
VpaK11BI GGWCC 9 cut(s) 1195, 1200, 1578, 1842, 1871, 3446, 4542, 4943, 5443
VspI ATTAAT 2 cut(s) 1674, 4874
XbaI TCTAGA 2 cut(s) 697, 3394
XceI RCATGY 3 cut(s) 98, 3433, 4431
XhoI CTCGAG 2 cut(s) 112, 3225
XmnI GAANNNNTTC 8 cut(s) 633, 1355, 1502, 2834, 3090, 3341, 4115, 4520
Zsp2I ATGCAT 3 cut(s) 1564, 2984, 4433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.