Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0001994

Physical Location & Seq

Genomic Coordinates Reverse (-)

15883190 .. 15890463

Gene Structure 7274 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG178500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGGCCTCCTGTGACGACGACTTCTCTCTCCTCGGCAGTAACGACTCAAACCCTAACCACCGCAACCCTCATGCGCCTCCCAACTCCCACCGCATTCCCCAGCCCTACGCGCCTCGCTTCGCCACCCGAACTACCCCGCCCGTGGTCACCGTCTCCAGCGGCGACAAGGCCAACGCCGGAGAAGATCAGGACGACGGCGAGGACTACGGAGACGCCGCCGCGTTCTGTTCCAACCCCTTCGACAGCGGCACGGATCAGAACGACGTCGCGTCGAAGAGGAAGGACCAGTCCGATGAGCTCAGCGACGGAGCCGGCGGCGGTGGCGGAGGAGGTTCGTACACCATCATCAGGAGATCGAAATCCTCGGCCGTGGCCGCCGGAGAGTACCGGAAGGATCGCGAGGAGTGGAGCGACGGCGCGATCGCTTGCCTTCTCGATGCGTACATGGACAAGTTCACGCAGCTGAATCGGGGAAATCTGCGAGGCCGGGATTGGGAGGAGGTGGCCGTGATCGTGAGCCAGAGGTCCGAGAGGCAAACCAAGAGCGTCGAACAGTGTAAGAACAAGGTCGATAATTTGAAGAAGAGGTACAAGCTCGAGAGGCACAGGATGACCAATTCTGGAAATTCGCCGAGTCATTGGCCTTGGTTCAAGAGAATGGAGCAGATCGTCGGCAATTCGCTTCCGGCGAAGGCCTCCTCTGATGATGACAGAGGCGTTACTTCCTCCGGTCCCACGCCTCAGAAATCAAAGAGAAATGCAAGTGCAATTACTAGTCCTGTGACTCAGATAAACAAGCAGCCGAAATTCAGTCTTAAATGGCGAAGAGTGGTGTTCAAAGTCAGTGGTGCTGCTCTAGCTGGCACTGCTGCTAACAACATTGACCCAAAGATAACAATGCTGATTGCGAGAGAAGTTGCAATGGCCTGCCGCAGTGGGGTGGAGGTGGCAATTGTTGTTGGTGGTCGCAACTTCTTTTGCGGAAATACGTGGGTGAATGAAACAGGTTTAGACAGAAGTACTGCATACCAAACTGGCATGATGACCACTGTAATGAATTCGATATTGCTCCAGTCAGCATTTGAGAAGATGGCAATCCAGACTCGTGTTCAAAGTGCATTTTCTCTGCCAGAGCTTGCTGAACCATACAATAGGCAACGGGCCATCCGGCATCTTGAAAAAGGCAGGGTTGTAATATTTGCTGGAATTGGTGCTGGCACTGGAAATCCACTCTTTACGACTGACACAGCTGCAGCACTTAGAGCTTCAGAAATCCATGCAGAGGCAGTACTCAAGGGTACCAATGTTGATGGTGTGTATGACCGCAATTCTCAAGACAATAATTTTACGTTCCAGCACATCTCTTTTAGGGAGTTGGTCACCAGAGGTGAGCACAACATGGACACAATGGCACTAACCTTATGTGAGGACAACAGGATTCCAGTTGTGATCTTTAATCTACTAGAGACTGGAAATATATCAAAAGCACTTCGTGGAGAACAAGTTGGTACCCTGATTGATCAGGTTGGAAGTATGAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AA_kinase Myb_DNA-bind_4

Protein Analysis

513

Amino Acids

56.01

Weight (kDa)

8.08

Isoelectric Point (pI)

40.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-bind_4	PF13837	133 - 223	8.4e-17	Myb/SANT-like DNA-binding domain
MADF_DNA_bdg	PF10545	157 - 221	5.5e-06	Alcohol dehydrogenase transcription factor Myb/SANT-like
AA_kinase	PF00696	276 - 486	1.3e-27	Amino acid kinase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001994)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10030 AT3G10030
fragaria_vesca	FvH4_6g17490 FvH4_6g17490 FvH4_6g17490 FvH4_6g17490
malus_domestica	MD12G1097000.v1.1 MD14G1094100.v1.1
prunus_persica	Prupe.7G120600_v2.0.a1 Prupe.7G120600_v2.0.a1 Prupe.7G120600_v2.0.a1 Prupe.7G120600_v2.0.a1 Prupe.7G120600_v2.0.a1 Prupe.7G120600_v2.0.a1
pyrus_communis	pycom14g09190
rosa_chinensis	RchiOBHm_Chr2g0173511 RchiOBHm_Chr3g0471731 RchiOBHm_Chr5g0071181
rosa_laevigata	RLG00000015608 RLG00000024138 RLG00000024278 RLG00000036093
rosa_multiflora	Rmu_sc0002476.1_g000019 Rmu_sc0004577.1_g000005 Rmu_sc0004577.1_g000006 Rmu_sc0013806.1_g000003 Rmu_sc0040530.1_g000001 Rmu_sc0041385.1_g000001 Rmu_ssc0000126.1_g000037 Rmu_ssc0000126.1_g000038
rosa_roxburghii	Rroxscaffold_3G00223840 Rroxscaffold_3G00265600 Rroxscaffold_6G00393240 Rroxscaffold_6G00409600
rosa_rugosa	Rorug03G0119300 Rorug03G0119400
rosa_samantha	Rh1CG168000 Rh2AG649600 Rh2DG555500 Rh2DG675400 Rh3BG196400 Rh3DG178500 Rh4BG099200 Rh4BG396300
rosa_wichuraiana	Rw0G009050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	267
Acc65I	GGTACC	2 cut(s)	1298, 1508
AccB1I	GGYRCC	2 cut(s)	1298, 1508
AccII	CGCG	5 cut(s)	110, 221, 269, 399, 419
AclWI	GGATC	2 cut(s)	261, 402
AcoI	YGGCCR	3 cut(s)	366, 372, 504
AcsI	RAATTY	3 cut(s)	625, 806, 1057
AcuI	CTGAAG	1 cut(s)	1251
AcyI	GRCGYC	2 cut(s)	213, 264
AdeI	CACNNNGTG	1 cut(s)	1493
AfaI	GTAC	8 cut(s)	338, 386, 443, 590, 1021, 1290, 1300, 1510
AfiI	CCNNNNNNNGG	5 cut(s)	142, 348, 493, 937, 1282
AgsI	TTSAA	5 cut(s)	580, 652, 838, 1112, 1178
AhdI	GACNNNNNGTC	1 cut(s)	268
AhlI	ACTAGT	1 cut(s)	773
AluBI	AGCT	7 cut(s)	298, 463, 595, 860, 1135, 1250, 1265
AluI	AGCT	7 cut(s)	298, 463, 595, 860, 1135, 1250, 1265
Alw21I	GWGCWC	2 cut(s)	300, 1395
Alw26I	GTCTC	3 cut(s)	157, 204, 1460
AlwI	GGATC	2 cut(s)	261, 402
Ama87I	CYCGRG	1 cut(s)	596
ApeKI	GCWGC	6 cut(s)	460, 799, 851, 869, 1250, 1253
ApoI	RAATTY	3 cut(s)	625, 806, 1057
AsiSI	GCGATCGC	1 cut(s)	423
Asp718I	GGTACC	2 cut(s)	1298, 1508
AspLEI	GCGC	3 cut(s)	76, 112, 419
AspS9I	GGNCC	4 cut(s)	283, 525, 731, 1161
AsuC2I	CCSGG	1 cut(s)	488
AsuHPI	GGTGA	4 cut(s)	139, 1006, 1372, 1400
AvaI	CYCGRG	1 cut(s)	596
AvaII	GGWCC	3 cut(s)	283, 525, 731
BaeI	ACNNNNGTAYC	2 cut(s)	1290, 1323
BanI	GGYRCC	2 cut(s)	1298, 1508
BanII	GRGCYC	1 cut(s)	300
BarI	GAAGNNNNNNTAC	2 cut(s)	572, 604
BauI	CACGAG	1 cut(s)	1104
Bbv12I	GWGCWC	2 cut(s)	300, 1395
BbvI	GCAGC	6 cut(s)	472, 811, 838, 856, 1237, 1265
BccI	CCATC	4 cut(s)	350, 1084, 1172, 1304
BceAI	ACGGC	4 cut(s)	211, 353, 430, 491
BcgI	CGANNNNNNTGC	2 cut(s)	449, 483
BclI	TGATCA	1 cut(s)	1519
BcnI	CCSGG	1 cut(s)	488
BcoDI	GTCTC	3 cut(s)	157, 204, 1460
BcuI	ACTAGT	1 cut(s)	773
BfaI	CTAG	3 cut(s)	774, 857, 1463
BfmI	CTRYAG	1 cut(s)	1251
BlpI	GCTNAGC	1 cut(s)	299
BmcAI	AGTACT	2 cut(s)	1021, 1290
Bme1390I	CCNGG	1 cut(s)	488
Bme18I	GGWCC	3 cut(s)	283, 525, 731
BmeRI	GACNNNNNGTC	1 cut(s)	268
BmeT110I	CYCGRG	1 cut(s)	596
BmgT120I	GGNCC	4 cut(s)	283, 525, 731, 1161
BmiI	GGNNCC	4 cut(s)	310, 733, 1300, 1510
BmrFI	CCNGG	1 cut(s)	488
BmsI	GCATC	2 cut(s)	427, 1180
BpmI	CTGGAG	2 cut(s)	139, 1055
Bpu1102I	GCTNAGC	1 cut(s)	299
BpuEI	CTTGAG	2 cut(s)	1277, 1317
BpuMI	CCSGG	1 cut(s)	488
BsaAI	YACGTR	1 cut(s)	990
BsaBI	GATNNNNATC	2 cut(s)	358, 1094
BsaHI	GRCGYC	2 cut(s)	213, 264
BsaJI	CCNNGG	5 cut(s)	31, 141, 363, 369, 644
BsaWI	WCCGGW	2 cut(s)	387, 728
BsaXI	ACNNNNNCTCC	2 cut(s)	1488, 1518
Bsc4I	CCNNNNNNNGG	5 cut(s)	142, 348, 493, 937, 1282
Bse118I	RCCGGY	1 cut(s)	311
Bse1I	ACTGG	6 cut(s)	286, 1039, 1072, 1225, 1442, 1474
Bse3DI	GCAATG	1 cut(s)	927
Bse8I	GATNNNNATC	2 cut(s)	358, 1094
BseDI	CCNNGG	5 cut(s)	31, 141, 363, 369, 644
BseGI	GGATG	2 cut(s)	615, 1164
BseJI	GATNNNNATC	2 cut(s)	358, 1094
BseLI	CCNNNNNNNGG	5 cut(s)	142, 348, 493, 937, 1282
BseMI	GCAATG	1 cut(s)	927
BseMII	CTCAG	3 cut(s)	313, 755, 800
BseNI	ACTGG	6 cut(s)	286, 1039, 1072, 1225, 1442, 1474
BseRI	GAGGAG	5 cut(s)	20, 342, 416, 512, 688
BseX3I	CGGCCG	1 cut(s)	366
BseXI	GCAGC	6 cut(s)	472, 811, 838, 856, 1237, 1265
BseYI	CCCAGC	1 cut(s)	99
Bsh1236I	CGCG	5 cut(s)	110, 221, 269, 399, 419
Bsh1285I	CGRYCG	2 cut(s)	369, 423
BshNI	GGYRCC	2 cut(s)	1298, 1508
BsiEI	CGRYCG	2 cut(s)	369, 423
BsiHKAI	GWGCWC	2 cut(s)	300, 1395
BsiHKCI	CYCGRG	1 cut(s)	596
BsiSI	CCGG	8 cut(s)	177, 312, 378, 388, 487, 686, 729, 1168
BslFI	GGGAC	1 cut(s)	717
BslI	CCNNNNNNNGG	5 cut(s)	142, 348, 493, 937, 1282
BsmAI	GTCTC	3 cut(s)	157, 204, 1460
BsmBI	CGTCTC	2 cut(s)	157, 204
BsmFI	GGGAC	1 cut(s)	717
BsmI	GAATGC	1 cut(s)	93
BsoBI	CYCGRG	1 cut(s)	596
Bsp1286I	GDGCHC	2 cut(s)	300, 1395
Bsp143I	GATC	9 cut(s)	184, 253, 353, 394, 420, 510, 666, 1449, 1519
Bsp1720I	GCTNAGC	1 cut(s)	299
Bsp68I	TCGCGA	1 cut(s)	399
BspCNI	CTCAG	3 cut(s)	312, 754, 799
BspFNI	CGCG	5 cut(s)	110, 221, 269, 399, 419
BspLI	GGNNCC	4 cut(s)	310, 733, 1300, 1510
BspMAI	CTGCAG	1 cut(s)	1255
BspPI	GGATC	2 cut(s)	261, 402
BspT107I	GGYRCC	2 cut(s)	1298, 1508
BsrDI	GCAATG	1 cut(s)	927
BsrFI	RCCGGY	1 cut(s)	311
BsrI	ACTGG	6 cut(s)	286, 1039, 1072, 1225, 1442, 1474
BssAI	RCCGGY	1 cut(s)	311
BssECI	CCNNGG	5 cut(s)	31, 141, 363, 369, 644
BssMI	GATC	9 cut(s)	184, 253, 353, 394, 420, 510, 666, 1449, 1519
BssNI	GRCGYC	2 cut(s)	213, 264
BssSI	CACGAG	1 cut(s)	1104
BssT1I	CCWWGG	1 cut(s)	644
Bst2BI	CACGAG	1 cut(s)	1104
Bst4CI	ACNGT	3 cut(s)	151, 555, 1051
Bst6I	CTCTTC	3 cut(s)	269, 578, 820
BstACI	GRCGYC	2 cut(s)	213, 264
BstBAI	YACGTR	1 cut(s)	990
BstC8I	GCNNGC	6 cut(s)	313, 427, 862, 928, 1137, 1216
BstDEI	CTNAG	4 cut(s)	299, 741, 786, 1259
BstDSI	CCRYGG	2 cut(s)	141, 369
BstEII	GGTNACC	2 cut(s)	145, 1378
BstF5I	GGATG	2 cut(s)	615, 1164
BstFNI	CGCG	5 cut(s)	110, 221, 269, 399, 419
BstHHI	GCGC	3 cut(s)	76, 112, 419
BstKTI	GATC	9 cut(s)	187, 256, 356, 397, 423, 513, 669, 1452, 1522
BstMAI	GTCTC	3 cut(s)	157, 204, 1460
BstMBI	GATC	9 cut(s)	184, 253, 353, 394, 420, 510, 666, 1449, 1519
BstMCI	CGRYCG	2 cut(s)	369, 423
BstMWI	GCNNNNNNNGC	7 cut(s)	109, 321, 374, 601, 857, 866, 1262
BstPI	GGTNACC	2 cut(s)	145, 1378
BstSCI	CCNGG	1 cut(s)	486
BstSFI	CTRYAG	1 cut(s)	1251
BstUI	CGCG	5 cut(s)	110, 221, 269, 399, 419
BstV1I	GCAGC	6 cut(s)	472, 811, 838, 856, 1237, 1265
BstZI	CGGCCG	1 cut(s)	366
BtgI	CCRYGG	2 cut(s)	141, 369
BtsCI	GGATG	2 cut(s)	615, 1164
BtsI	GCAGTG	2 cut(s)	864, 940
BtsIMutI	CAGTG	6 cut(s)	560, 850, 864, 940, 1047, 1218
BtuMI	TCGCGA	1 cut(s)	399
Cac8I	GCNNGC	6 cut(s)	313, 427, 862, 928, 1137, 1216
CfoI	GCGC	3 cut(s)	76, 112, 419
Cfr10I	RCCGGY	1 cut(s)	311
Cfr13I	GGNCC	4 cut(s)	283, 525, 731, 1161
CseI	GACGC	3 cut(s)	221, 258, 535
Csp6I	GTAC	8 cut(s)	337, 385, 442, 589, 1020, 1289, 1299, 1509
CspCI	CAANNNNNGTGG	2 cut(s)	826, 861
CviAII	CATG	5 cut(s)	71, 445, 1039, 1277, 1399
CviQI	GTAC	8 cut(s)	337, 385, 442, 589, 1020, 1289, 1299, 1509
DdeI	CTNAG	4 cut(s)	299, 741, 786, 1259
DpnI	GATC	9 cut(s)	186, 255, 355, 396, 422, 512, 668, 1451, 1521
DpnII	GATC	9 cut(s)	184, 253, 353, 394, 420, 510, 666, 1449, 1519
DraIII	CACNNNGTG	1 cut(s)	1493
DriI	GACNNNNNGTC	1 cut(s)	268
EaeI	YGGCCR	3 cut(s)	366, 372, 504
EagI	CGGCCG	1 cut(s)	366
Eam1104I	CTCTTC	3 cut(s)	269, 578, 820
Eam1105I	GACNNNNNGTC	1 cut(s)	268
EarI	CTCTTC	3 cut(s)	269, 578, 820
EciI	GGCGGA	1 cut(s)	339
Ecl136II	GAGCTC	1 cut(s)	298
EclXI	CGGCCG	1 cut(s)	366
Eco130I	CCWWGG	1 cut(s)	644
Eco147I	AGGCCT	1 cut(s)	695
Eco24I	GRGCYC	1 cut(s)	300
Eco47I	GGWCC	3 cut(s)	283, 525, 731
Eco52I	CGGCCG	1 cut(s)	366
Eco53kI	GAGCTC	1 cut(s)	298
Eco57I	CTGAAG	1 cut(s)	1251
Eco88I	CYCGRG	1 cut(s)	596
Eco91I	GGTNACC	2 cut(s)	145, 1378
EcoICRI	GAGCTC	1 cut(s)	298
EcoO65I	GGTNACC	2 cut(s)	145, 1378
EcoRI	GAATTC	1 cut(s)	1057
EcoT14I	CCWWGG	1 cut(s)	644
EcoT38I	GRGCYC	1 cut(s)	300
ErhI	CCWWGG	1 cut(s)	644
Esp3I	CGTCTC	2 cut(s)	157, 204
FaeI	CATG	5 cut(s)	74, 448, 1042, 1280, 1402
FaqI	GGGAC	1 cut(s)	717
FatI	CATG	5 cut(s)	70, 444, 1038, 1276, 1398
FauI	CCCGC	1 cut(s)	144
FbaI	TGATCA	1 cut(s)	1519
FokI	GGATG	2 cut(s)	622, 1151
FriOI	GRGCYC	1 cut(s)	300
FspBI	CTAG	3 cut(s)	774, 857, 1463
GlaI	GCGC	3 cut(s)	75, 111, 418
GsaI	CCCAGC	1 cut(s)	103
GsuI	CTGGAG	2 cut(s)	139, 1055
HapII	CCGG	8 cut(s)	177, 312, 378, 388, 487, 686, 729, 1168
HgaI	GACGC	3 cut(s)	221, 258, 535
HhaI	GCGC	3 cut(s)	76, 112, 419
Hin1I	GRCGYC	2 cut(s)	213, 264
Hin1II	CATG	5 cut(s)	74, 448, 1042, 1280, 1402
Hin6I	GCGC	3 cut(s)	74, 110, 417
HinP1I	GCGC	3 cut(s)	74, 110, 417
HinfI	GANTC	6 cut(s)	44, 466, 634, 784, 1102, 1438
HpaII	CCGG	8 cut(s)	177, 312, 378, 388, 487, 686, 729, 1168
HphI	GGTGA	4 cut(s)	139, 1006, 1372, 1400
Hpy166II	GTNNAC	2 cut(s)	339, 456
Hpy188I	TCNGA	7 cut(s)	258, 292, 529, 703, 744, 789, 1270
Hpy8I	GTNNAC	2 cut(s)	339, 456
Hpy99I	CGWCG	9 cut(s)	20, 197, 266, 269, 274, 308, 416, 551, 674
HpyAV	CCTTC	5 cut(s)	247, 274, 385, 440, 685
HpyCH4III	ACNGT	3 cut(s)	151, 555, 1051
HpyCH4IV	ACGT	3 cut(s)	264, 989, 1349
HpyCH4V	TGCA	7 cut(s)	761, 767, 920, 1025, 1118, 1253, 1280
HpyF10VI	GCNNNNNNNGC	7 cut(s)	109, 321, 374, 601, 857, 866, 1262
HpyF3I	CTNAG	4 cut(s)	299, 741, 786, 1259
HpySE526I	ACGT	3 cut(s)	264, 989, 1349
Hsp92I	GRCGYC	2 cut(s)	213, 264
Hsp92II	CATG	5 cut(s)	74, 448, 1042, 1280, 1402
HspAI	GCGC	3 cut(s)	74, 110, 417
KpnI	GGTACC	2 cut(s)	1302, 1512
KroI	GCCGGC	1 cut(s)	311
KroNI	GCCGGC	1 cut(s)	313
Ksp22I	TGATCA	1 cut(s)	1519
Kzo9I	GATC	9 cut(s)	184, 253, 353, 394, 420, 510, 666, 1449, 1519
LmnI	GCTCC	4 cut(s)	308, 408, 661, 1074
Lsp1109I	GCAGC	6 cut(s)	472, 811, 838, 856, 1237, 1265
LweI	GCATC	2 cut(s)	427, 1180
MaeI	CTAG	3 cut(s)	774, 857, 1463
MaeII	ACGT	3 cut(s)	264, 989, 1349
MaeIII	GTNAC	6 cut(s)	11, 38, 145, 718, 781, 1378
MalI	GATC	9 cut(s)	186, 255, 355, 396, 422, 512, 668, 1451, 1521
MboI	GATC	9 cut(s)	184, 253, 353, 394, 420, 510, 666, 1449, 1519
MboII	GAAGA	6 cut(s)	194, 286, 592, 595, 837, 1099
MfeI	CAATTG	1 cut(s)	951
MhlI	GDGCHC	2 cut(s)	300, 1395
MlyI	GAGTC	4 cut(s)	38, 643, 778, 1096
MmeI	TCCRAC	2 cut(s)	255, 1507
MroNI	GCCGGC	1 cut(s)	311
MseI	TTAA	3 cut(s)	816, 1455, 1540
MslI	CAYNNNNRTG	1 cut(s)	1052
MspA1I	CMGCKG	4 cut(s)	159, 246, 463, 1250
MspI	CCGG	8 cut(s)	177, 312, 378, 388, 487, 686, 729, 1168
MspR9I	CCNGG	1 cut(s)	488
MunI	CAATTG	1 cut(s)	951
Mva1269I	GAATGC	1 cut(s)	93
MvnI	CGCG	5 cut(s)	110, 221, 269, 399, 419
MwoI	GCNNNNNNNGC	7 cut(s)	109, 321, 374, 601, 857, 866, 1262
NaeI	GCCGGC	1 cut(s)	313
NciI	CCSGG	1 cut(s)	488
NdeII	GATC	9 cut(s)	184, 253, 353, 394, 420, 510, 666, 1449, 1519
NgoMIV	GCCGGC	1 cut(s)	311
NlaIII	CATG	5 cut(s)	74, 448, 1042, 1280, 1402
NlaIV	GGNNCC	4 cut(s)	310, 733, 1300, 1510
NmeAIII	GCCGAG	3 cut(s)	12, 344, 657
NmuCI	GTSAC	4 cut(s)	11, 145, 781, 1378
NruI	TCGCGA	1 cut(s)	399
PaeR7I	CTCGAG	1 cut(s)	596
PceI	AGGCCT	1 cut(s)	695
PcsI	WCGNNNNNNNCGW	2 cut(s)	39, 686
PctI	GAATGC	1 cut(s)	93
PdiI	GCCGGC	1 cut(s)	313
PfeI	GAWTC	2 cut(s)	466, 1438
Ple19I	CGATCG	1 cut(s)	423
PleI	GAGTC	4 cut(s)	38, 642, 778, 1096
PpsI	GAGTC	4 cut(s)	38, 642, 778, 1096
Ppu21I	YACGTR	1 cut(s)	990
Psp124BI	GAGCTC	1 cut(s)	300
PspEI	GGTNACC	2 cut(s)	145, 1378
PspFI	CCCAGC	1 cut(s)	99
PspN4I	GGNNCC	4 cut(s)	310, 733, 1300, 1510
PspPI	GGNCC	4 cut(s)	283, 525, 731, 1161
PstI	CTGCAG	1 cut(s)	1255
PvuI	CGATCG	1 cut(s)	423
PvuII	CAGCTG	2 cut(s)	463, 1250
RgaI	GCGATCGC	1 cut(s)	423
RruI	TCGCGA	1 cut(s)	399
RsaI	GTAC	8 cut(s)	338, 386, 443, 590, 1021, 1290, 1300, 1510
RsaNI	GTAC	8 cut(s)	337, 385, 442, 589, 1020, 1289, 1299, 1509
RseI	CAYNNNNRTG	1 cut(s)	1052
SacI	GAGCTC	1 cut(s)	300
SaqAI	TTAA	3 cut(s)	816, 1455, 1540
Sau3AI	GATC	9 cut(s)	184, 253, 353, 394, 420, 510, 666, 1449, 1519
Sau96I	GGNCC	4 cut(s)	283, 525, 731, 1161
ScaI	AGTACT	2 cut(s)	1021, 1290
SchI	GAGTC	4 cut(s)	38, 643, 778, 1096
ScrFI	CCNGG	1 cut(s)	488
SduI	GDGCHC	2 cut(s)	300, 1395
SfaAI	GCGATCGC	1 cut(s)	423
SfaNI	GCATC	2 cut(s)	427, 1180
SfcI	CTRYAG	1 cut(s)	1251
Sfr274I	CTCGAG	1 cut(s)	596
SgfI	GCGATCGC	1 cut(s)	423
SinI	GGWCC	3 cut(s)	283, 525, 731
SlaI	CTCGAG	1 cut(s)	596
SmiMI	CAYNNNNRTG	1 cut(s)	1052
SmlI	CTYRAG	3 cut(s)	596, 1292, 1332
SmoI	CTYRAG	3 cut(s)	596, 1292, 1332
SpeI	ACTAGT	1 cut(s)	773
SseBI	AGGCCT	1 cut(s)	695
SspI	AATATT	1 cut(s)	1197
SspMI	CTAG	3 cut(s)	774, 857, 1463
SstI	GAGCTC	1 cut(s)	300
StuI	AGGCCT	1 cut(s)	695
StyD4I	CCNGG	1 cut(s)	486
StyI	CCWWGG	1 cut(s)	644
TaaI	ACNGT	3 cut(s)	151, 555, 1051
TaiI	ACGT	3 cut(s)	267, 992, 1352
TaqI	TCGA	8 cut(s)	240, 272, 356, 435, 549, 570, 597, 1061
TatI	WGTACW	2 cut(s)	1019, 1288
TauI	GCSGC	7 cut(s)	162, 218, 221, 249, 318, 377, 933
TfiI	GAWTC	2 cut(s)	466, 1438
Tru1I	TTAA	3 cut(s)	816, 1455, 1540
Tru9I	TTAA	3 cut(s)	816, 1455, 1540
TscAI	CASTG	6 cut(s)	560, 850, 871, 940, 1054, 1225
TseFI	GTSAC	4 cut(s)	11, 145, 781, 1378
TseI	GCWGC	6 cut(s)	460, 799, 851, 869, 1250, 1253
Tsp45I	GTSAC	4 cut(s)	11, 145, 781, 1378
TspDTI	ATGAA	2 cut(s)	1014, 1070
TspGWI	ACGGA	3 cut(s)	222, 266, 321
TspRI	CASTG	6 cut(s)	560, 850, 871, 940, 1054, 1225
VpaK11BI	GGWCC	3 cut(s)	283, 525, 731
XapI	RAATTY	3 cut(s)	625, 806, 1057
XhoI	CTCGAG	1 cut(s)	596
XspI	CTAG	3 cut(s)	774, 857, 1463
ZraI	GACGTC	1 cut(s)	265
ZrmI	AGTACT	2 cut(s)	1021, 1290

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.