Rh4DG061600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004225
Physical Location & Seq
Forward (+)
9242777 .. 9243421
645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG061600.1

Sequence Viewer

Length: 507 bp
ATGAAATTACCAAGTCACCATCCTTCACTTGGCTACCCGAAACAACGTGTGGCTCGTTCTTCCCCGAGATCTGATGAGGCCATTGATGGGTCTAGGTTTTACAGTCTTGAAGGAAGCTTTTACAACTCGAACATCAACATGCCCGAAGAGTCAAGAGATGCAATTTGGATCCTCTCACGGTTGGCCAGTTTTGTTGTCAACCGGCTCGAGTTTATGCTCTCAACGTACTCTATTCTTATCTGGGATTCGGCTTCCCCTCCCACCACTCAAGACTCTTCCAGATATACTTGGCATTACATATCAGACAGATCTCAACATGCTACAGCTGCAGTAGTTGAACTCACTGGAACTCTGGAAGAGCTGAAGATCAAAGTGGCTGAGGAGGCTATAATACAAAAGAATATTGCAGATCAAGAAGATGCAGAATGCTTTATCTTGCATAAATTGCAGCCAAGGGAAAATGTCCATACCTCGCCGTTTATTGTTGGCTGTTCCTCTGACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

19.05

Weight (kDa)

5.48

Isoelectric Point (pI)

72.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 163, 176
AcoI YGGCCR 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 383
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 2 cut(s) 29, 87
AflIII ACRYGT 1 cut(s) 46
AgsI TTSAA 2 cut(s) 110, 338
AluBI AGCT 3 cut(s) 117, 326, 361
AluI AGCT 3 cut(s) 117, 326, 361
AlwI GGATC 2 cut(s) 163, 176
Ama87I CYCGRG 2 cut(s) 64, 206
AoxI GGCC 2 cut(s) 78, 183
ApeKI GCWGC 2 cut(s) 326, 448
AsuHPI GGTGA 1 cut(s) 8
AvaI CYCGRG 2 cut(s) 64, 206
BalI TGGCCA 1 cut(s) 185
BamHI GGATCC 1 cut(s) 168
BbvCI CCTCAGC 1 cut(s) 378
BbvI GCAGC 2 cut(s) 313, 460
BccI CCATC 2 cut(s) 27, 80
BceAI ACGGC 1 cut(s) 460
BfaI CTAG 1 cut(s) 93
BfmI CTRYAG 2 cut(s) 321, 327
BglII AGATCT 2 cut(s) 68, 308
BisI GCNGC 2 cut(s) 327, 449
BlsI GCNGC 2 cut(s) 328, 450
BmeT110I CYCGRG 2 cut(s) 64, 206
BmiI GGNNCC 1 cut(s) 170
BmsI GCATC 2 cut(s) 148, 409
Bpu10I CCTNAGC 1 cut(s) 378
BpuEI CTTGAG 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 452
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 87
Bse118I RCCGGY 1 cut(s) 201
Bse1I ACTGG 3 cut(s) 186, 349, 502
BseDI CCNNGG 1 cut(s) 452
BseGI GGATG 1 cut(s) 19
BseLI CCNNNNNNNGG 2 cut(s) 29, 87
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 3 cut(s) 186, 349, 502
BseRI GAGGAG 1 cut(s) 395
BseXI GCAGC 2 cut(s) 313, 460
BshFI GGCC 2 cut(s) 80, 185
BsiHKCI CYCGRG 2 cut(s) 64, 206
BsiSI CCGG 1 cut(s) 202
BslI CCNNNNNNNGG 2 cut(s) 29, 87
BsmI GAATGC 1 cut(s) 431
BsnI GGCC 2 cut(s) 80, 185
BsoBI CYCGRG 2 cut(s) 64, 206
Bsp143I GATC 5 cut(s) 68, 168, 308, 366, 409
BspANI GGCC 2 cut(s) 80, 185
BspCNI CTCAG 1 cut(s) 370
BspLI GGNNCC 1 cut(s) 170
BspMAI CTGCAG 1 cut(s) 331
BspPI GGATC 2 cut(s) 163, 176
BspQI GCTCTTC 1 cut(s) 351
BsrFI RCCGGY 1 cut(s) 201
BsrI ACTGG 3 cut(s) 186, 349, 502
BssAI RCCGGY 1 cut(s) 201
BssECI CCNNGG 1 cut(s) 452
BssMI GATC 5 cut(s) 68, 168, 308, 366, 409
BssT1I CCWWGG 1 cut(s) 452
Bst4CI ACNGT 2 cut(s) 104, 180
Bst6I CTCTTC 3 cut(s) 141, 280, 351
BstAPI GCANNNNNTGC 1 cut(s) 445
BstDEI CTNAG 1 cut(s) 378
BstF5I GGATG 1 cut(s) 19
BstKTI GATC 5 cut(s) 71, 171, 311, 369, 412
BstMBI GATC 5 cut(s) 68, 168, 308, 366, 409
BstMWI GCNNNNNNNGC 3 cut(s) 326, 383, 445
BstNSI RCATGY 2 cut(s) 142, 320
BstSFI CTRYAG 2 cut(s) 321, 327
BstV1I GCAGC 2 cut(s) 313, 460
BstX2I RGATCY 3 cut(s) 68, 168, 308
BstYI RGATCY 3 cut(s) 68, 168, 308
BsuRI GGCC 2 cut(s) 80, 185
BtsCI GGATG 1 cut(s) 19
BtsIMutI CAGTG 1 cut(s) 342
Cfr10I RCCGGY 1 cut(s) 201
Csp6I GTAC 1 cut(s) 226
CviAII CATG 2 cut(s) 139, 317
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 1 cut(s) 378
DpnI GATC 5 cut(s) 70, 170, 310, 368, 411
DpnII GATC 5 cut(s) 68, 168, 308, 366, 409
EaeI YGGCCR 1 cut(s) 183
Eam1104I CTCTTC 3 cut(s) 141, 280, 351
EarI CTCTTC 3 cut(s) 141, 280, 351
Eco130I CCWWGG 1 cut(s) 452
Eco57I CTGAAG 1 cut(s) 383
Eco88I CYCGRG 2 cut(s) 64, 206
EcoT14I CCWWGG 1 cut(s) 452
ErhI CCWWGG 1 cut(s) 452
FaeI CATG 2 cut(s) 142, 320
FaiI YATR 8 cut(s) 140, 215, 285, 299, 318, 389, 441, 468
FatI CATG 2 cut(s) 138, 316
Fnu4HI GCNGC 2 cut(s) 327, 449
FokI GGATG 1 cut(s) 6
Fsp4HI GCNGC 2 cut(s) 327, 449
FspBI CTAG 1 cut(s) 93
GluI GCNGC 2 cut(s) 327, 449
HaeIII GGCC 2 cut(s) 80, 185
HapII CCGG 1 cut(s) 202
Hin1II CATG 2 cut(s) 142, 320
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HindIII AAGCTT 1 cut(s) 115
HinfI GANTC 3 cut(s) 149, 245, 272
HpaII CCGG 1 cut(s) 202
HphI GGTGA 1 cut(s) 8
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 3 cut(s) 73, 304, 499
Hpy188III TCNNGA 6 cut(s) 107, 153, 269, 279, 353, 413
Hpy8I GTNNAC 1 cut(s) 199
HpyAV CCTTC 2 cut(s) 33, 104
HpyCH4III ACNGT 2 cut(s) 104, 180
HpyCH4IV ACGT 2 cut(s) 46, 224
HpyCH4V TGCA 6 cut(s) 161, 329, 407, 422, 439, 448
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 383, 445
HpyF3I CTNAG 1 cut(s) 378
HpySE526I ACGT 2 cut(s) 46, 224
Hsp92II CATG 2 cut(s) 142, 320
Kzo9I GATC 5 cut(s) 68, 168, 308, 366, 409
LguI GCTCTTC 1 cut(s) 351
LpnPI CCDG 6 cut(s) 199, 215, 226, 292, 330, 338
Lsp1109I GCAGC 2 cut(s) 313, 460
LweI GCATC 2 cut(s) 148, 409
MaeI CTAG 1 cut(s) 93
MaeII ACGT 2 cut(s) 46, 224
MaeIII GTNAC 1 cut(s) 14
MalI GATC 5 cut(s) 70, 170, 310, 368, 411
MboI GATC 5 cut(s) 68, 168, 308, 366, 409
MboII GAAGA 6 cut(s) 51, 158, 267, 368, 376, 428
MflI RGATCY 3 cut(s) 68, 168, 308
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 3 cut(s) 5, 162, 443
MluNI TGGCCA 1 cut(s) 185
MlyI GAGTC 2 cut(s) 158, 266
MnlI CCTC 7 cut(s) 70, 182, 267, 373, 376, 481, 505
Mox20I TGGCCA 1 cut(s) 185
MscI TGGCCA 1 cut(s) 185
MslI CAYNNNNRTG 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 1 cut(s) 202
Mva1269I GAATGC 1 cut(s) 431
MwoI GCNNNNNNNGC 3 cut(s) 326, 383, 445
NdeII GATC 5 cut(s) 68, 168, 308, 366, 409
NlaIII CATG 2 cut(s) 142, 320
NlaIV GGNNCC 1 cut(s) 170
NmuCI GTSAC 1 cut(s) 14
NspI RCATGY 2 cut(s) 142, 320
PaeR7I CTCGAG 1 cut(s) 206
PciSI GCTCTTC 1 cut(s) 351
PcsI WCGNNNNNNNCGW 1 cut(s) 52
PctI GAATGC 1 cut(s) 431
PfeI GAWTC 1 cut(s) 245
PkrI GCNGC 2 cut(s) 328, 450
PleI GAGTC 2 cut(s) 157, 266
PpsI GAGTC 2 cut(s) 157, 266
PspN4I GGNNCC 1 cut(s) 170
PspXI VCTCGAGB 1 cut(s) 206
PstI CTGCAG 1 cut(s) 331
PsuI RGATCY 3 cut(s) 68, 168, 308
PvuII CAGCTG 1 cut(s) 326
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
RseI CAYNNNNRTG 1 cut(s) 137
SapI GCTCTTC 1 cut(s) 351
SatI GCNGC 2 cut(s) 327, 449
Sau3AI GATC 5 cut(s) 68, 168, 308, 366, 409
SchI GAGTC 2 cut(s) 158, 266
SetI ASST 7 cut(s) 49, 98, 119, 227, 328, 363, 473
SfaNI GCATC 2 cut(s) 148, 409
SfcI CTRYAG 2 cut(s) 321, 327
Sfr274I CTCGAG 1 cut(s) 206
SlaI CTCGAG 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 2 cut(s) 206, 267
SmoI CTYRAG 2 cut(s) 206, 267
Sse9I AATT 3 cut(s) 5, 162, 443
SspI AATATT 1 cut(s) 403
SspMI CTAG 1 cut(s) 93
StyI CCWWGG 1 cut(s) 452
TaaI ACNGT 2 cut(s) 104, 180
TaiI ACGT 2 cut(s) 49, 227
TaqI TCGA 2 cut(s) 128, 207
TasI AATT 3 cut(s) 5, 162, 443
TfiI GAWTC 1 cut(s) 245
TscAI CASTG 1 cut(s) 349
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 2 cut(s) 326, 448
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 349
XceI RCATGY 2 cut(s) 142, 320
XcmI CCANNNNNNNNNTGG 1 cut(s) 26
XhoI CTCGAG 1 cut(s) 206
XspI CTAG 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.