Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0000936

Physical Location & Seq

Genomic Coordinates Reverse (-)

22119796 .. 22125947

Gene Structure 6152 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG217400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

ATGCTCACAGATATTAGAGTTGTTCGCGAGTCTAATGATGATGACCACTTGGTTTTGGAGTTGGGACTGAATTTTCTGACAGCTGATGATATGATTGCAATACTTGCTGTGAAGTTGAGGAAAAGACTAGGTTTCGGGATGTGGGCAAAGTTGCATATTACAGGCATGCATGTTGAAGGGAAGCTGTTGATTGGGGTTAAGTTTCTTCGTAGGTGGCCTTTCCTTGGCCGTTTGCGGATATGCTTCATTGAGCCTCCATATTTTCAGATGACAGTCAAGCCTATTTTTACACATGGCCTTGATGTTACAGTTCTTCCAGGAATTGCTGGGTGGCTGGAAAAGCTTCTTTCCATTGCCTTTGAGCAGACCCTTGTTGAGCCAAATATGCTGGTTGTTGACATGGAGAAATTCGCCTCACCGCAGCAAGAGAGTTGGTTCTCTGTGAATGAAAAGGAATCTCTTGGACATGTCAGAGTAGAAGTTATTGAAGCATCTGACATCAAAGCTGCAGATCTAAATGGATTATCTGACCCTTATGTTAAGGGTCAATTGGGCCTTTACCGGTTCAGGACGAAGACACAAAAGAAAACACTGGCTCCAAAATGGCATGAAGAATTCAAGATGCCCATTATTACATGGGATTCATCTACTGTGCTATCTATTGAAGTTCGTGACAAGGACCGATTTGTTGATGATTGCCTTGGATTCTGCTGCATTAACATTGGTGAGTATAGAGATGGCCAGAGGCATGACATGTGGTTGCCTCTTCAGAATATTAAAACAGGAAGGATCCATCTTGCAGTAACTGTGGTTGAAGATAATCCCAAGGGGGGTGATAATCCAAATGTTGAGGAAACACCAAATGTGGAAGATGAAAGAAAAAGGTTTGCCAATGACACTAGTAATAAAAGTTCCTTCTCATCTGCATCATCAGAAAAGTTTGACAAAGTGGCAGATAACTTTGAGCCAATTGATGTTGATGGGCAAAGAGAGACTGGAATATGGGTCCATCACCCAGGAAGTGAAGTTTCACAAACTTGGGAGAGTAGAAAAGGAAAGAGTCGGCTCCTTGATAATGAGATTCAAAATGATACTGGTGGCCATAGTTCAGTCACCTCTGTCTCCCCAACTAATGAGGACAGTGGCACTGAAAAGAATCATGAGGATAAACACAGGATGAGAGCAGTTCGCAAGGGTCTAAACAGGATCTGCTCGGTATTCCACAAGAATTCCAAAAAGGAGAATGATTCAAGCAGCTTTAGAGAGACTGTTGGATCTGGACATGCTAATATTCAGGAAATTAGTGAAAAAGATATTGCGGTAAGATTTATTGTGGAGGATGATCTTTCCAACCCTCAAGAGGGCAAGGGTTTGAAGGAAGAAGGGATTGTCACTTCTGGAGGGAGTGGTTCAAAGGGAAAGGGCAAGTGCAAGGGTTTAGACAAGGCAAAGAGTTTCCTTAAACCAGCAAGAAAATCTTCTCGAGATTTTAGGAAGTGTCAAAGTGATTCTTACAAAGGGAGGGAAAGAAAAACTTTAGAAGAATTTAACTCTACTGATGAGGATGATGCTCCTCCAAAACAGTTGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

C2 SMP_LBD

Protein Analysis

530

Amino Acids

59.83

Weight (kDa)

6.47

Isoelectric Point (pI)

37.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SMP_MUG190-like	PF25669	58 - 137	8.1e-08	MUG190-like SMP domain
C2	PF00168	155 - 256	2e-22	C2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000936)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53590 AT3G14590 AT3G14590 AT3G14590 AT3G14590 AT3G14590
fragaria_vesca	FvH4_3g16980 FvH4_3g16980 FvH4_3g16980 FvH4_3g16980 FvH4_3g16980 FvH4_3g17020 FvH4_3g17020 FvH4_3g17020 FvH4_3g17020 FvH4_3g17020 FvH4_3g17020
malus_domestica	MD00G1185500.v1.1 MD00G1185600.v1.1 MD03G1254200.v1.1 MD03G1254900.v1.1 MD11G1275800.v1.1 MD11G1276000.v1.1
prunus_persica	Prupe.4G152200_v2.0.a1 Prupe.4G152200_v2.0.a1 Prupe.4G152300_v2.0.a1 Prupe.4G152300_v2.0.a1 Prupe.4G152300_v2.0.a1 Prupe.4G152300_v2.0.a1 Prupe.4G152300_v2.0.a1 Prupe.4G152300_v2.0.a1 Prupe.4G152300_v2.0.a1
pyrus_communis	pycom03g20170 pycom03g20190 pycom11g24400
rosa_chinensis	RchiOBHm_Chr2g0135571 RchiOBHm_Chr2g0159501 RchiOBHm_Chr2g0159511 RchiOBHm_Chr5g0028301 RchiOBHm_Chr5g0028351 RchiOBHm_Chr5g0039631
rosa_laevigata	RLG00000033080 RLG00000033088
rosa_multiflora	Rmu_sc0001020.1_g000001 Rmu_sc0002481.1_g000040 Rmu_sc0008526.1_g000010
rosa_roxburghii	Rroxscaffold_1G00051350 Rroxscaffold_1G00051420 Rroxscaffold_1G00051430
rosa_rugosa	Rorug05G0105600
rosa_samantha	Rh1AG292900 Rh4AG059100 Rh5AG199100 Rh5AG199600 Rh5BG197700 Rh5BG198100 Rh5CG217400 Rh5CG218100 Rh5DG199400 Rh5DG200000 Rh7DG370100
rosa_wichuraiana	Rw4G016870 Rw5G018160 Rw5G018190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	27
AciI	CCGC	3 cut(s)	235, 419, 1319
AclWI	GGATC	4 cut(s)	784, 797, 1214, 1282
AcoI	YGGCCR	3 cut(s)	226, 739, 1099
AcsI	RAATTY	5 cut(s)	70, 407, 614, 1228, 1544
AcuI	CTGAAG	1 cut(s)	752
AfiI	CCNNNNNNNGG	4 cut(s)	224, 830, 1360, 1361
AflIII	ACRYGT	2 cut(s)	466, 753
AgeI	ACCGGT	1 cut(s)	561
AhlI	ACTAGT	1 cut(s)	899
AjnI	CCWGG	2 cut(s)	316, 1015
AluBI	AGCT	5 cut(s)	83, 184, 343, 506, 1257
AluI	AGCT	5 cut(s)	83, 184, 343, 506, 1257
Alw26I	GTCTC	3 cut(s)	986, 1126, 1259
AlwI	GGATC	4 cut(s)	784, 797, 1214, 1282
AlwNI	CAGNNNCTG	2 cut(s)	806, 1209
Ama87I	CYCGRG	1 cut(s)	1482
AoxI	GGCC	6 cut(s)	215, 226, 295, 553, 739, 1099
ApeKI	GCWGC	4 cut(s)	421, 506, 711, 1254
ApoI	RAATTY	5 cut(s)	70, 407, 614, 1228, 1544
AsiGI	ACCGGT	1 cut(s)	561
Asp700I	GAANNNNTTC	2 cut(s)	342, 1477
AspS9I	GGNCC	3 cut(s)	553, 679, 1006
AsuHPI	GGTGA	5 cut(s)	408, 737, 845, 1004, 1105
AvaI	CYCGRG	1 cut(s)	1482
AvaII	GGWCC	2 cut(s)	679, 1006
BalI	TGGCCA	2 cut(s)	741, 1101
BamHI	GGATCC	1 cut(s)	789
BbsI	GAAGAC	1 cut(s)	581
BbvI	GCAGC	4 cut(s)	433, 493, 698, 1266
BccI	CCATC	4 cut(s)	731, 801, 974, 1017
BceAI	ACGGC	1 cut(s)	213
BciT130I	CCWGG	2 cut(s)	318, 1017
BcoDI	GTCTC	3 cut(s)	986, 1126, 1259
BcuI	ACTAGT	1 cut(s)	899
BfaI	CTAG	2 cut(s)	128, 900
BfmI	CTRYAG	1 cut(s)	507
BglII	AGATCT	1 cut(s)	511
BisI	GCNGC	4 cut(s)	422, 507, 712, 1255
BlsI	GCNGC	4 cut(s)	423, 508, 713, 1256
Bme1390I	CCNGG	2 cut(s)	318, 1017
Bme18I	GGWCC	2 cut(s)	679, 1006
BmeT110I	CYCGRG	1 cut(s)	1482
BmgT120I	GGNCC	3 cut(s)	553, 679, 1006
BmiI	GGNNCC	4 cut(s)	597, 791, 1007, 1067
BmrFI	CCNGG	2 cut(s)	318, 1017
BmsI	GCATC	4 cut(s)	500, 612, 935, 1558
BpiI	GAAGAC	1 cut(s)	581
BpmI	CTGGAG	1 cut(s)	1419
BpuEI	CTTGAG	1 cut(s)	1341
BsaJI	CCNNGG	4 cut(s)	223, 700, 825, 1015
BsaWI	WCCGGW	1 cut(s)	561
BsaXI	ACNNNNNCTCC	2 cut(s)	580, 610
Bsc4I	CCNNNNNNNGG	4 cut(s)	224, 830, 1360, 1361
Bse118I	RCCGGY	1 cut(s)	561
Bse1I	ACTGG	3 cut(s)	597, 1000, 1099
Bse3DI	GCAATG	1 cut(s)	351
BseBI	CCWGG	2 cut(s)	318, 1017
BseDI	CCNNGG	4 cut(s)	223, 700, 825, 1015
BseGI	GGATG	4 cut(s)	144, 1182, 1345, 1570
BseLI	CCNNNNNNNGG	4 cut(s)	224, 830, 1360, 1361
BseMI	GCAATG	1 cut(s)	351
BseNI	ACTGG	3 cut(s)	597, 1000, 1099
BseRI	GAGGAG	1 cut(s)	1563
BseXI	GCAGC	4 cut(s)	433, 493, 698, 1266
BseYI	CCCAGC	1 cut(s)	326
Bsh1236I	CGCG	1 cut(s)	27
BshFI	GGCC	6 cut(s)	217, 228, 297, 555, 741, 1101
BshTI	ACCGGT	1 cut(s)	561
BsiHKCI	CYCGRG	1 cut(s)	1482
BsiSI	CCGG	1 cut(s)	562
BslFI	GGGAC	1 cut(s)	78
BslI	CCNNNNNNNGG	4 cut(s)	224, 830, 1360, 1361
BsmAI	GTCTC	3 cut(s)	986, 1126, 1259
BsmFI	GGGAC	1 cut(s)	78
BsnI	GGCC	6 cut(s)	217, 228, 297, 555, 741, 1101
BsoBI	CYCGRG	1 cut(s)	1482
Bsp143I	GATC	5 cut(s)	511, 789, 1206, 1274, 1342
Bsp68I	TCGCGA	1 cut(s)	27
BspACI	CCGC	3 cut(s)	235, 419, 1319
BspANI	GGCC	6 cut(s)	217, 228, 297, 555, 741, 1101
BspFNI	CGCG	1 cut(s)	27
BspHI	TCATGA	1 cut(s)	1159
BspLI	GGNNCC	4 cut(s)	597, 791, 1007, 1067
BspMAI	CTGCAG	1 cut(s)	511
BspPI	GGATC	4 cut(s)	784, 797, 1214, 1282
BsrDI	GCAATG	1 cut(s)	351
BsrFI	RCCGGY	1 cut(s)	561
BsrI	ACTGG	3 cut(s)	597, 1000, 1099
BssAI	RCCGGY	1 cut(s)	561
BssECI	CCNNGG	4 cut(s)	223, 700, 825, 1015
BssMI	GATC	5 cut(s)	511, 789, 1206, 1274, 1342
BssT1I	CCWWGG	3 cut(s)	223, 700, 825
Bst2UI	CCWGG	2 cut(s)	318, 1017
Bst4CI	ACNGT	7 cut(s)	274, 310, 652, 808, 1142, 1270, 1584
Bst6I	CTCTTC	1 cut(s)	771
BstAPI	GCANNNNNTGC	1 cut(s)	104
BstC8I	GCNNGC	1 cut(s)	167
BstF5I	GGATG	4 cut(s)	144, 1182, 1345, 1570
BstFNI	CGCG	1 cut(s)	27
BstKTI	GATC	5 cut(s)	514, 792, 1209, 1277, 1345
BstMAI	GTCTC	3 cut(s)	986, 1126, 1259
BstMBI	GATC	5 cut(s)	511, 789, 1206, 1274, 1342
BstMWI	GCNNNNNNNGC	3 cut(s)	104, 340, 385
BstNI	CCWGG	2 cut(s)	318, 1017
BstNSI	RCATGY	5 cut(s)	169, 173, 470, 757, 1286
BstSCI	CCNGG	2 cut(s)	316, 1015
BstSFI	CTRYAG	1 cut(s)	507
BstUI	CGCG	1 cut(s)	27
BstV1I	GCAGC	4 cut(s)	433, 493, 698, 1266
BstV2I	GAAGAC	1 cut(s)	581
BstX2I	RGATCY	4 cut(s)	511, 789, 1206, 1274
BstYI	RGATCY	4 cut(s)	511, 789, 1206, 1274
BsuRI	GGCC	6 cut(s)	217, 228, 297, 555, 741, 1101
BtsCI	GGATG	4 cut(s)	144, 1182, 1345, 1570
BtsIMutI	CAGTG	3 cut(s)	590, 1146, 1147
BtuMI	TCGCGA	1 cut(s)	27
Cac8I	GCNNGC	1 cut(s)	167
CaiI	CAGNNNCTG	2 cut(s)	806, 1209
CciI	TCATGA	1 cut(s)	1159
Cfr10I	RCCGGY	1 cut(s)	561
Cfr13I	GGNCC	3 cut(s)	553, 679, 1006
CspAI	ACCGGT	1 cut(s)	561
DpnI	GATC	5 cut(s)	513, 791, 1208, 1276, 1344
DpnII	GATC	5 cut(s)	511, 789, 1206, 1274, 1342
EaeI	YGGCCR	3 cut(s)	226, 739, 1099
Eam1104I	CTCTTC	1 cut(s)	771
EarI	CTCTTC	1 cut(s)	771
Eco130I	CCWWGG	3 cut(s)	223, 700, 825
Eco47I	GGWCC	2 cut(s)	679, 1006
Eco57I	CTGAAG	1 cut(s)	752
Eco88I	CYCGRG	1 cut(s)	1482
EcoRI	GAATTC	2 cut(s)	614, 1228
EcoRII	CCWGG	2 cut(s)	316, 1015
EcoT14I	CCWWGG	3 cut(s)	223, 700, 825
EcoT22I	ATGCAT	1 cut(s)	171
ErhI	CCWWGG	3 cut(s)	223, 700, 825
FalI	AAGNNNNNCTT	6 cut(s)	1462, 1494, 1495, 1527, 1519, 1551
FaqI	GGGAC	1 cut(s)	78
Fnu4HI	GCNGC	4 cut(s)	422, 507, 712, 1255
FokI	GGATG	4 cut(s)	151, 1189, 1352, 1577
Fsp4HI	GCNGC	4 cut(s)	422, 507, 712, 1255
FspBI	CTAG	2 cut(s)	128, 900
GluI	GCNGC	4 cut(s)	422, 507, 712, 1255
GsaI	CCCAGC	1 cut(s)	330
GsuI	CTGGAG	1 cut(s)	1419
HaeIII	GGCC	6 cut(s)	217, 228, 297, 555, 741, 1101
HapII	CCGG	1 cut(s)	562
HincII	GTYRAC	1 cut(s)	397
HindII	GTYRAC	1 cut(s)	397
HindIII	AAGCTT	1 cut(s)	341
HinfI	GANTC	9 cut(s)	29, 455, 641, 705, 1060, 1081, 1156, 1247, 1508
HpaII	CCGG	1 cut(s)	562
HphI	GGTGA	5 cut(s)	408, 737, 845, 1004, 1105
Hpy166II	GTNNAC	1 cut(s)	397
Hpy188I	TCNGA	7 cut(s)	78, 267, 473, 496, 529, 771, 934
Hpy8I	GTNNAC	1 cut(s)	397
HpyAV	CCTTC	5 cut(s)	170, 780, 925, 1369, 1376
HpyCH4III	ACNGT	7 cut(s)	274, 310, 652, 808, 1142, 1270, 1584
HpyCH4V	TGCA	8 cut(s)	98, 154, 169, 509, 714, 800, 926, 1431
HpyF10VI	GCNNNNNNNGC	3 cut(s)	104, 340, 385
Kzo9I	GATC	5 cut(s)	511, 789, 1206, 1274, 1342
LmnI	GCTCC	3 cut(s)	601, 1071, 1576
Lsp1109I	GCAGC	4 cut(s)	433, 493, 698, 1266
LweI	GCATC	4 cut(s)	500, 612, 935, 1558
MaeI	CTAG	2 cut(s)	128, 900
MaeIII	GTNAC	5 cut(s)	304, 671, 802, 1111, 1390
MalI	GATC	5 cut(s)	513, 791, 1208, 1276, 1344
MboI	GATC	5 cut(s)	511, 789, 1206, 1274, 1342
MfeI	CAATTG	2 cut(s)	548, 969
MflI	RGATCY	4 cut(s)	511, 789, 1206, 1274
MlsI	TGGCCA	2 cut(s)	741, 1101
MluCI	AATT	9 cut(s)	70, 321, 407, 548, 614, 969, 1228, 1299, 1544
MluNI	TGGCCA	2 cut(s)	741, 1101
MlyI	GAGTC	2 cut(s)	38, 1069
MmeI	TCCRAC	2 cut(s)	1252, 1374
Mox20I	TGGCCA	2 cut(s)	741, 1101
Mph1103I	ATGCAT	1 cut(s)	171
MroXI	GAANNNNTTC	2 cut(s)	342, 1477
MscI	TGGCCA	2 cut(s)	741, 1101
MseI	TTAA	6 cut(s)	198, 540, 717, 777, 1461, 1548
Msp20I	TGGCCA	2 cut(s)	741, 1101
MspA1I	CMGCKG	1 cut(s)	83
MspI	CCGG	1 cut(s)	562
MspR9I	CCNGG	2 cut(s)	318, 1017
MunI	CAATTG	2 cut(s)	548, 969
MvaI	CCWGG	2 cut(s)	318, 1017
MvnI	CGCG	1 cut(s)	27
MwoI	GCNNNNNNNGC	3 cut(s)	104, 340, 385
NdeII	GATC	5 cut(s)	511, 789, 1206, 1274, 1342
NlaIV	GGNNCC	4 cut(s)	597, 791, 1007, 1067
NmuCI	GTSAC	3 cut(s)	671, 1111, 1390
NruI	TCGCGA	1 cut(s)	27
NsiI	ATGCAT	1 cut(s)	171
NspI	RCATGY	5 cut(s)	169, 173, 470, 757, 1286
PaeI	GCATGC	1 cut(s)	169
PaeR7I	CTCGAG	1 cut(s)	1482
PagI	TCATGA	1 cut(s)	1159
PciI	ACATGT	2 cut(s)	466, 753
PdmI	GAANNNNTTC	2 cut(s)	342, 1477
PfeI	GAWTC	7 cut(s)	455, 641, 705, 1081, 1156, 1247, 1508
PfoI	TCCNGGA	1 cut(s)	316
PinAI	ACCGGT	1 cut(s)	561
PkrI	GCNGC	4 cut(s)	423, 508, 713, 1256
PleI	GAGTC	2 cut(s)	37, 1068
PpsI	GAGTC	2 cut(s)	37, 1068
PscI	ACATGT	2 cut(s)	466, 753
Psp6I	CCWGG	2 cut(s)	316, 1015
PspFI	CCCAGC	1 cut(s)	326
PspGI	CCWGG	2 cut(s)	316, 1015
PspN4I	GGNNCC	4 cut(s)	597, 791, 1007, 1067
PspPI	GGNCC	3 cut(s)	553, 679, 1006
PsrI	GAACNNNNNNTAC	2 cut(s)	895, 927
PstI	CTGCAG	1 cut(s)	511
PstNI	CAGNNNCTG	2 cut(s)	806, 1209
PsuI	RGATCY	4 cut(s)	511, 789, 1206, 1274
PvuII	CAGCTG	1 cut(s)	83
RruI	TCGCGA	1 cut(s)	27
SaqAI	TTAA	6 cut(s)	198, 540, 717, 777, 1461, 1548
SatI	GCNGC	4 cut(s)	422, 507, 712, 1255
Sau3AI	GATC	5 cut(s)	511, 789, 1206, 1274, 1342
Sau96I	GGNCC	3 cut(s)	553, 679, 1006
SchI	GAGTC	2 cut(s)	38, 1069
ScrFI	CCNGG	2 cut(s)	318, 1017
SetI	ASST	9 cut(s)	85, 133, 186, 215, 345, 508, 887, 1118, 1259
SfaNI	GCATC	4 cut(s)	500, 612, 935, 1558
SfcI	CTRYAG	1 cut(s)	507
Sfr274I	CTCGAG	1 cut(s)	1482
SinI	GGWCC	2 cut(s)	679, 1006
SlaI	CTCGAG	1 cut(s)	1482
SmlI	CTYRAG	2 cut(s)	1356, 1482
SmoI	CTYRAG	2 cut(s)	1356, 1482
SpeI	ACTAGT	1 cut(s)	899
SphI	GCATGC	1 cut(s)	169
Sse9I	AATT	9 cut(s)	70, 321, 407, 548, 614, 969, 1228, 1299, 1544
SsiI	CCGC	3 cut(s)	235, 419, 1319
SspI	AATATT	2 cut(s)	775, 1291
SspMI	CTAG	2 cut(s)	128, 900
StyD4I	CCNGG	2 cut(s)	316, 1015
StyI	CCWWGG	3 cut(s)	223, 700, 825
TaaI	ACNGT	7 cut(s)	274, 310, 652, 808, 1142, 1270, 1584
TaqI	TCGA	1 cut(s)	1483
TaqII	GACCGA	1 cut(s)	696
TasI	AATT	9 cut(s)	70, 321, 407, 548, 614, 969, 1228, 1299, 1544
TfiI	GAWTC	7 cut(s)	455, 641, 705, 1081, 1156, 1247, 1508
Tru1I	TTAA	6 cut(s)	198, 540, 717, 777, 1461, 1548
Tru9I	TTAA	6 cut(s)	198, 540, 717, 777, 1461, 1548
TscAI	CASTG	3 cut(s)	597, 1147, 1153
TseFI	GTSAC	3 cut(s)	671, 1111, 1390
TseI	GCWGC	4 cut(s)	421, 506, 711, 1254
Tsp45I	GTSAC	3 cut(s)	671, 1111, 1390
TspDTI	ATGAA	5 cut(s)	235, 462, 624, 633, 888
TspRI	CASTG	3 cut(s)	597, 1147, 1153
VpaK11BI	GGWCC	2 cut(s)	679, 1006
XapI	RAATTY	5 cut(s)	70, 407, 614, 1228, 1544
XceI	RCATGY	5 cut(s)	169, 173, 470, 757, 1286
XhoI	CTCGAG	1 cut(s)	1482
XmnI	GAANNNNTTC	2 cut(s)	342, 1477
XspI	CTAG	2 cut(s)	128, 900
Zsp2I	ATGCAT	1 cut(s)	171

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.