Rh5DG437000

Carbohydrate-binding protein of the ER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001437
Physical Location & Seq
Forward (+)
69682926 .. 69685465
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG437000.1

Sequence Viewer

Length: 1545 bp
ATGGCTAGTAGCTCCATAGTAGTCTTACTCTTCCCACTTTTAGCTCTAGCTCTATTATCTGCATTGGCAGCCGATAATTTTCTGAACATAGATTGCGGGTCATCTGATTCGTACACCGATAAAAATTTGATCAACTGGGTTGGAGAGGATGCCTTTGTTCAGAACGGCAAGTCTCAAGTGGTGCAGATCCCCAATGGAGTCTCTCAAGAAATGAGCACTTTGAGAGTGTTTACAACCAGGAAGAAAAACTGTTACTCCATTGATGCAGAAAAGGGAGCTCAGTTTCTTGTGCGTGGAAGTTTCTACTACGGAAATTATGACAAAAAATCAGCTCCTCCTTCCTTTGATGTTCTTTTGGATGGGAATTTTTTTGACACAATTCAGACTACAGCTGAGGATGTTATTTATTTTGAAGCTATATATGTCGCAAGAAGCGGCTCTATACAGCTATGTCTTGCTCAATCTAAGCCTAACCAGTTTCCATTTATATCCGCGATTGAGATCCGGGGCCTGGATCCAACAATGTACTCTCATTTTGAGTCGAACTATATGATGGTAAATACAAGGAGGGTTGCTTTTGGTGCAACTAAATCTATAAGGTATCCAGATGATGGTTATGATCGTTATTGGGATCCATCAGGGGATGTCACCGGCATACTGAAGGTCACAAGTGATGCACCAGGAATTGATGGCATTGTGGAAGATCAGCCTCCAAAACTAGCTATGCAAACTGCAATAACCACCTCAAAAACATCACAACCATTGGGTATAATTGGCAATGGTCTTCCAAGTGCTGAAGTCCCCATTTACATCACTATGTTCTTCTCTGAAGTGAATCTACTTGATTCGTCAACTGATGTCAGATCCTTTCAGATTTTCATTGATGACAAGAATCCTTCAGATCCCATCATTCCCATCTATGGAGGTGTGACAGAAATGTACATTGCAAACAGGACCGGCTCTTCGAAGACCTCAATTTCTCTGGTGGCCACCACTGATTCGACGCTGCCTCCTCTGATCAATGCCATGGAAGTTTACTACCTTCTAGGGCCACTAACAGAGGGAACAAATAGCAAAGATGTTGAAGGGTTGGCTGCATTGCAAGGAAAGTTCAGTATTCTACAGGAATGGACTGGAGACCCTTGCCTCCCATCTCCATATACATGGGACTGGGTCAATTGTACTACTGATGACACACCGCGAGTAACAGCACTGTATTTGAATGGCTATGGTCTATCTGGGCAACTTCCAGACTTCAGTTCCATGGATGCTCTTGTCACAATAGACATGCACAACAACAGCCTGAGTGGAGCAATTCCTAGTTTTCTTGGCACCTTGCCCAATCTAAAAGAACTGAACGTAGGAGACAATAAACTGAGTGGACCGGTACCCTCCACAATTTCAAAGAATACCAAGCTGAAGTTCGAGGATTCTGGCAATCCTAATCTATGTGCGTCTGGCGAGTCCTGTAAGACAATTGCTAGCAATGCCCCTCTTCATCAGGCAGCAGTGGCACTAAGAAGAAAAGCAGCAAACTACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Malectin_like

Protein Analysis

514

Amino Acids

55.84

Weight (kDa)

4.7

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 30 - 347 2.4e-78 Malectin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1387
AccB1I GGYRCC 2 cut(s) 1331, 1387
AccB7I CCANNNNNTGG 1 cut(s) 611
AccII CGCG 2 cut(s) 494, 1201
AciI CCGC 4 cut(s) 96, 435, 492, 1199
AclWI GGATC 8 cut(s) 181, 496, 509, 522, 626, 639, 858, 896
AcoI YGGCCR 1 cut(s) 987
AcsI RAATTY 2 cut(s) 124, 364
AcuI CTGAAG 6 cut(s) 680, 816, 849, 882, 1240, 1439
AfaI GTAC 5 cut(s) 113, 527, 941, 1183, 1389
AfiI CCNNNNNNNGG 3 cut(s) 511, 611, 920
AgeI ACCGGT 1 cut(s) 1384
AgsI TTSAA 4 cut(s) 413, 1085, 1222, 1404
AjnI CCWGG 3 cut(s) 236, 510, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 1406, 1438
Alw21I GWGCWC 2 cut(s) 218, 280
Alw26I GTCTC 4 cut(s) 177, 205, 1131, 1359
AlwI GGATC 8 cut(s) 181, 496, 509, 522, 626, 639, 858, 896
AoxI GGCC 3 cut(s) 508, 987, 1049
ApeKI GCWGC 5 cut(s) 68, 1006, 1094, 1505, 1529
ApoI RAATTY 2 cut(s) 124, 364
ArsI GACNNNNNNTTYG 2 cut(s) 946, 978
AsiGI ACCGGT 1 cut(s) 1384
Asp718I GGTACC 1 cut(s) 1387
AspS9I GGNCC 4 cut(s) 508, 954, 1049, 1382
AsuC2I CCSGG 1 cut(s) 506
AsuHPI GGTGA 1 cut(s) 640
AsuII TTCGAA 1 cut(s) 965
AsuNHI GCTAGC 1 cut(s) 1481
AvaII GGWCC 2 cut(s) 954, 1382
BalI TGGCCA 1 cut(s) 989
BamHI GGATCC 2 cut(s) 514, 631
BanI GGYRCC 2 cut(s) 1331, 1387
BanII GRGCYC 1 cut(s) 280
BarI GAAGNNNNNNTAC 2 cut(s) 822, 854
BbsI GAAGAC 2 cut(s) 776, 974
Bbv12I GWGCWC 2 cut(s) 218, 280
BbvCI CCTCAGC 1 cut(s) 393
BbvI GCAGC 5 cut(s) 80, 993, 1081, 1517, 1541
BccI CCATC 8 cut(s) 353, 547, 605, 643, 683, 914, 923, 1159
BceAI ACGGC 1 cut(s) 181
BciT130I CCWGG 3 cut(s) 238, 512, 681
BciVI GTATCC 1 cut(s) 612
BclI TGATCA 2 cut(s) 129, 1017
BcnI CCSGG 1 cut(s) 506
BcoDI GTCTC 4 cut(s) 177, 205, 1131, 1359
BfaI CTAG 6 cut(s) 6, 47, 719, 1046, 1320, 1482
BfmI CTRYAG 2 cut(s) 387, 1121
BfuI GTATCC 1 cut(s) 612
BisI GCNGC 6 cut(s) 69, 436, 1007, 1095, 1506, 1530
BlsI GCNGC 6 cut(s) 70, 437, 1008, 1096, 1507, 1531
Bme1390I CCNGG 4 cut(s) 238, 506, 512, 681
Bme18I GGWCC 2 cut(s) 954, 1382
BmgT120I GGNCC 4 cut(s) 508, 954, 1049, 1382
BmiI GGNNCC 5 cut(s) 509, 516, 633, 1333, 1389
BmrFI CCNGG 4 cut(s) 238, 506, 512, 681
BmrI ACTGGG 2 cut(s) 145, 1180
BmsI GCATC 4 cut(s) 139, 253, 664, 1258
BmtI GCTAGC 1 cut(s) 1485
BmuI ACTGGG 2 cut(s) 145, 1180
BpiI GAAGAC 2 cut(s) 776, 974
BpmI CTGGAG 1 cut(s) 1155
Bpu10I CCTNAGC 1 cut(s) 393
Bpu14I TTCGAA 1 cut(s) 965
BpuEI CTTGAG 2 cut(s) 159, 189
BpuMI CCSGG 1 cut(s) 506
BsaBI GATNNNNATC 1 cut(s) 500
BsaI GGTCTC 1 cut(s) 1131
BsaJI CCNNGG 3 cut(s) 505, 1026, 1263
BsaWI WCCGGW 1 cut(s) 1384
BsaXI ACNNNNNCTCC 4 cut(s) 239, 269, 994, 1024
Bsc4I CCNNNNNNNGG 3 cut(s) 511, 611, 920
Bse118I RCCGGY 3 cut(s) 650, 956, 1384
Bse1I ACTGG 4 cut(s) 140, 475, 1138, 1175
Bse3DI GCAATG 4 cut(s) 784, 942, 1097, 1492
Bse8I GATNNNNATC 1 cut(s) 500
BseBI CCWGG 3 cut(s) 238, 512, 681
BseDI CCNNGG 3 cut(s) 505, 1026, 1263
BseGI GGATG 5 cut(s) 154, 364, 403, 649, 1273
BseJI GATNNNNATC 1 cut(s) 500
BseLI CCNNNNNNNGG 3 cut(s) 511, 611, 920
BseMI GCAATG 4 cut(s) 784, 942, 1097, 1492
BseMII CTCAG 4 cut(s) 293, 384, 1295, 1367
BseNI ACTGG 4 cut(s) 140, 475, 1138, 1175
BseRI GAGGAG 2 cut(s) 324, 1002
BseXI GCAGC 5 cut(s) 80, 993, 1081, 1517, 1541
BsgI GTGCAG 1 cut(s) 203
Bsh1236I CGCG 2 cut(s) 494, 1201
BshFI GGCC 3 cut(s) 510, 989, 1051
BshNI GGYRCC 2 cut(s) 1331, 1387
BshTI ACCGGT 1 cut(s) 1384
BsiHKAI GWGCWC 2 cut(s) 218, 280
BsiSI CCGG 4 cut(s) 505, 651, 957, 1385
BslFI GGGAC 2 cut(s) 785, 1181
BslI CCNNNNNNNGG 3 cut(s) 511, 611, 920
BsmAI GTCTC 4 cut(s) 177, 205, 1131, 1359
BsmFI GGGAC 2 cut(s) 785, 1181
BsnI GGCC 3 cut(s) 510, 989, 1051
Bso31I GGTCTC 1 cut(s) 1131
Bsp119I TTCGAA 1 cut(s) 965
Bsp1286I GDGCHC 2 cut(s) 218, 280
Bsp1407I TGTACA 1 cut(s) 939
Bsp19I CCATGG 2 cut(s) 1026, 1263
BspACI CCGC 4 cut(s) 96, 435, 492, 1199
BspANI GGCC 3 cut(s) 510, 989, 1051
BspCNI CTCAG 4 cut(s) 292, 385, 1296, 1368
BspFNI CGCG 2 cut(s) 494, 1201
BspLI GGNNCC 5 cut(s) 509, 516, 633, 1333, 1389
BspOI GCTAGC 1 cut(s) 1485
BspPI GGATC 8 cut(s) 181, 496, 509, 522, 626, 639, 858, 896
BspQI GCTCTTC 1 cut(s) 967
BspT104I TTCGAA 1 cut(s) 965
BspT107I GGYRCC 2 cut(s) 1331, 1387
BspTNI GGTCTC 1 cut(s) 1131
BsrDI GCAATG 4 cut(s) 784, 942, 1097, 1492
BsrFI RCCGGY 3 cut(s) 650, 956, 1384
BsrGI TGTACA 1 cut(s) 939
BsrI ACTGG 4 cut(s) 140, 475, 1138, 1175
BssAI RCCGGY 3 cut(s) 650, 956, 1384
BssECI CCNNGG 3 cut(s) 505, 1026, 1263
BssT1I CCWWGG 2 cut(s) 1026, 1263
Bst2UI CCWGG 3 cut(s) 238, 512, 681
Bst4CI ACNGT 2 cut(s) 251, 1215
Bst6I CTCTTC 3 cut(s) 35, 967, 1500
BstAUI TGTACA 1 cut(s) 939
BstBI TTCGAA 1 cut(s) 965
BstC8I GCNNGC 1 cut(s) 1483
BstDEI CTNAG 6 cut(s) 279, 393, 465, 1304, 1376, 1517
BstDSI CCRYGG 2 cut(s) 1026, 1263
BstF5I GGATG 5 cut(s) 154, 364, 403, 649, 1273
BstFNI CGCG 2 cut(s) 494, 1201
BstMAI GTCTC 4 cut(s) 177, 205, 1131, 1359
BstMWI GCNNNNNNNGC 4 cut(s) 68, 581, 1487, 1511
BstNI CCWGG 3 cut(s) 238, 512, 681
BstNSI RCATGY 1 cut(s) 1291
BstSCI CCNGG 4 cut(s) 236, 504, 510, 679
BstSFI CTRYAG 2 cut(s) 387, 1121
BstUI CGCG 2 cut(s) 494, 1201
BstV1I GCAGC 5 cut(s) 80, 993, 1081, 1517, 1541
BstV2I GAAGAC 2 cut(s) 776, 974
BstX2I RGATCY 6 cut(s) 186, 501, 514, 631, 863, 901
BstXI CCANNNNNNTGG 1 cut(s) 1164
BstYI RGATCY 6 cut(s) 186, 501, 514, 631, 863, 901
BsuI GTATCC 1 cut(s) 612
BsuRI GGCC 3 cut(s) 510, 989, 1051
BtgI CCRYGG 2 cut(s) 1026, 1263
BtsCI GGATG 5 cut(s) 154, 364, 403, 649, 1273
BtsI GCAGTG 1 cut(s) 1515
BtsIMutI CAGTG 3 cut(s) 993, 1211, 1515
Cac8I GCNNGC 1 cut(s) 1483
Cfr10I RCCGGY 3 cut(s) 650, 956, 1384
Cfr13I GGNCC 4 cut(s) 508, 954, 1049, 1382
CseI GACGC 2 cut(s) 1012, 1443
Csp6I GTAC 5 cut(s) 112, 526, 940, 1182, 1388
CspAI ACCGGT 1 cut(s) 1384
CviAII CATG 4 cut(s) 1027, 1164, 1264, 1288
CviQI GTAC 5 cut(s) 112, 526, 940, 1182, 1388
DdeI CTNAG 6 cut(s) 279, 393, 465, 1304, 1376, 1517
EaeI YGGCCR 1 cut(s) 987
Eam1104I CTCTTC 3 cut(s) 35, 967, 1500
EarI CTCTTC 3 cut(s) 35, 967, 1500
Ecl136II GAGCTC 1 cut(s) 278
Eco130I CCWWGG 2 cut(s) 1026, 1263
Eco24I GRGCYC 1 cut(s) 280
Eco31I GGTCTC 1 cut(s) 1131
Eco47I GGWCC 2 cut(s) 954, 1382
Eco53kI GAGCTC 1 cut(s) 278
Eco57I CTGAAG 6 cut(s) 680, 816, 849, 882, 1240, 1439
EcoICRI GAGCTC 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 508
EcoRII CCWGG 3 cut(s) 236, 510, 679
EcoT14I CCWWGG 2 cut(s) 1026, 1263
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 2 cut(s) 1026, 1263
FaeI CATG 4 cut(s) 1030, 1167, 1267, 1291
FaqI GGGAC 2 cut(s) 785, 1181
FatI CATG 4 cut(s) 1026, 1163, 1263, 1287
FauI CCCGC 1 cut(s) 89
FbaI TGATCA 2 cut(s) 129, 1017
Fnu4HI GCNGC 6 cut(s) 69, 436, 1007, 1095, 1506, 1530
FokI GGATG 5 cut(s) 161, 371, 410, 656, 1280
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 6 cut(s) 69, 436, 1007, 1095, 1506, 1530
FspBI CTAG 6 cut(s) 6, 47, 719, 1046, 1320, 1482
GluI GCNGC 6 cut(s) 69, 436, 1007, 1095, 1506, 1530
GsuI CTGGAG 1 cut(s) 1155
HaeIII GGCC 3 cut(s) 510, 989, 1051
HapII CCGG 4 cut(s) 505, 651, 957, 1385
HgaI GACGC 2 cut(s) 1012, 1443
Hin1II CATG 4 cut(s) 1030, 1167, 1267, 1291
HincII GTYRAC 1 cut(s) 852
HindII GTYRAC 1 cut(s) 852
HinfI GANTC 9 cut(s) 107, 198, 539, 835, 845, 892, 998, 1430, 1463
HpaII CCGG 4 cut(s) 505, 651, 957, 1385
HphI GGTGA 1 cut(s) 640
Hpy166II GTNNAC 5 cut(s) 114, 231, 852, 1036, 1382
Hpy188I TCNGA 9 cut(s) 84, 106, 162, 384, 829, 863, 873, 901, 1017
Hpy188III TCNNGA 3 cut(s) 206, 605, 1250
Hpy8I GTNNAC 5 cut(s) 114, 231, 852, 1036, 1382
Hpy99I CGWCG 1 cut(s) 1006
HpyAV CCTTC 5 cut(s) 348, 655, 906, 1052, 1079
HpyCH4III ACNGT 2 cut(s) 251, 1215
HpyCH4IV ACGT 1 cut(s) 1359
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 581, 1487, 1511
HpyF3I CTNAG 6 cut(s) 279, 393, 465, 1304, 1376, 1517
HpySE526I ACGT 1 cut(s) 1359
Hsp92II CATG 4 cut(s) 1030, 1167, 1267, 1291
KpnI GGTACC 1 cut(s) 1391
Ksp22I TGATCA 2 cut(s) 129, 1017
LguI GCTCTTC 1 cut(s) 967
LmnI GCTCC 4 cut(s) 17, 275, 337, 1310
Lsp1109I GCAGC 5 cut(s) 80, 993, 1081, 1517, 1541
LweI GCATC 4 cut(s) 139, 253, 664, 1258
MaeI CTAG 6 cut(s) 6, 47, 719, 1046, 1320, 1482
MaeII ACGT 1 cut(s) 1359
MaeIII GTNAC 6 cut(s) 251, 646, 664, 928, 1204, 1276
MboII GAAGA 9 cut(s) 22, 253, 713, 776, 814, 954, 979, 1487, 1533
MfeI CAATTG 2 cut(s) 1177, 1476
MflI RGATCY 6 cut(s) 186, 501, 514, 631, 863, 901
MhlI GDGCHC 2 cut(s) 218, 280
MlsI TGGCCA 1 cut(s) 989
MluNI TGGCCA 1 cut(s) 989
MlyI GAGTC 3 cut(s) 207, 548, 1472
MmeI TCCRAC 2 cut(s) 121, 542
Mox20I TGGCCA 1 cut(s) 989
MscI TGGCCA 1 cut(s) 989
MseI TTAA 1 cut(s) 1543
MslI CAYNNNNRTG 2 cut(s) 815, 1162
Msp20I TGGCCA 1 cut(s) 989
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 4 cut(s) 505, 651, 957, 1385
MspR9I CCNGG 4 cut(s) 238, 506, 512, 681
MunI CAATTG 2 cut(s) 1177, 1476
MvaI CCWGG 3 cut(s) 238, 512, 681
MvnI CGCG 2 cut(s) 494, 1201
MwoI GCNNNNNNNGC 4 cut(s) 68, 581, 1487, 1511
NciI CCSGG 1 cut(s) 506
NcoI CCATGG 2 cut(s) 1026, 1263
NheI GCTAGC 1 cut(s) 1481
NlaIII CATG 4 cut(s) 1030, 1167, 1267, 1291
NlaIV GGNNCC 5 cut(s) 509, 516, 633, 1333, 1389
NmuCI GTSAC 4 cut(s) 646, 664, 928, 1276
NspI RCATGY 1 cut(s) 1291
NspV TTCGAA 1 cut(s) 965
PciSI GCTCTTC 1 cut(s) 967
PfeI GAWTC 6 cut(s) 107, 835, 845, 892, 998, 1430
PflFI GACNNNGTC 1 cut(s) 1172
PflMI CCANNNNNTGG 1 cut(s) 611
PinAI ACCGGT 1 cut(s) 1384
PkrI GCNGC 6 cut(s) 70, 437, 1008, 1096, 1507, 1531
PleI GAGTC 3 cut(s) 206, 547, 1471
PpsI GAGTC 3 cut(s) 206, 547, 1471
Psp124BI GAGCTC 1 cut(s) 280
Psp6I CCWGG 3 cut(s) 236, 510, 679
PspGI CCWGG 3 cut(s) 236, 510, 679
PspN4I GGNNCC 5 cut(s) 509, 516, 633, 1333, 1389
PspPI GGNCC 4 cut(s) 508, 954, 1049, 1382
PsuI RGATCY 6 cut(s) 186, 501, 514, 631, 863, 901
PsyI GACNNNGTC 1 cut(s) 1172
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 5 cut(s) 113, 527, 941, 1183, 1389
RsaNI GTAC 5 cut(s) 112, 526, 940, 1182, 1388
RseI CAYNNNNRTG 2 cut(s) 815, 1162
SacI GAGCTC 1 cut(s) 280
SapI GCTCTTC 1 cut(s) 967
SaqAI TTAA 1 cut(s) 1543
SatI GCNGC 6 cut(s) 69, 436, 1007, 1095, 1506, 1530
Sau96I GGNCC 4 cut(s) 508, 954, 1049, 1382
SchI GAGTC 3 cut(s) 207, 548, 1472
ScrFI CCNGG 4 cut(s) 238, 506, 512, 681
SduI GDGCHC 2 cut(s) 218, 280
SfaNI GCATC 4 cut(s) 139, 253, 664, 1258
SfcI CTRYAG 2 cut(s) 387, 1121
SfuI TTCGAA 1 cut(s) 965
SinI GGWCC 2 cut(s) 954, 1382
SmiMI CAYNNNNRTG 2 cut(s) 815, 1162
SmlI CTYRAG 2 cut(s) 174, 204
SmoI CTYRAG 2 cut(s) 174, 204
SsiI CCGC 4 cut(s) 96, 435, 492, 1199
SspMI CTAG 6 cut(s) 6, 47, 719, 1046, 1320, 1482
SstI GAGCTC 1 cut(s) 280
StyD4I CCNGG 4 cut(s) 236, 504, 510, 679
StyI CCWWGG 2 cut(s) 1026, 1263
TaaI ACNGT 2 cut(s) 251, 1215
TaiI ACGT 1 cut(s) 1362
TaqI TCGA 4 cut(s) 542, 965, 1001, 1425
TatI WGTACW 3 cut(s) 525, 939, 1181
TauI GCSGC 1 cut(s) 438
TfiI GAWTC 6 cut(s) 107, 835, 845, 892, 998, 1430
Tru1I TTAA 1 cut(s) 1543
Tru9I TTAA 1 cut(s) 1543
TscAI CASTG 3 cut(s) 1000, 1218, 1515
TseFI GTSAC 4 cut(s) 646, 664, 928, 1276
TseI GCWGC 5 cut(s) 68, 1006, 1094, 1505, 1529
Tsp45I GTSAC 4 cut(s) 646, 664, 928, 1276
TspDTI ATGAA 2 cut(s) 868, 1487
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 3 cut(s) 1000, 1218, 1515
Tth111I GACNNNGTC 1 cut(s) 1172
Van91I CCANNNNNTGG 1 cut(s) 611
VpaK11BI GGWCC 2 cut(s) 954, 1382
XapI RAATTY 2 cut(s) 124, 364
XceI RCATGY 1 cut(s) 1291
XspI CTAG 6 cut(s) 6, 47, 719, 1046, 1320, 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.