AT1G23950
WRKY Family

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001976
Physical Location & Seq
Reverse (-)
8469533 .. 8471417
1885 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G23950.4

Sequence Viewer

Length: 819 bp
ATGACGACGGAGGCGAATTCTACGGCGGAGGAGGGTTATAGTGTACAGAGAGACTTTTGGCGTCAAGCGGCGAAATCCGATGGTTTCGATTTAGAGAACATCTCACTTCCACCAGGTACGAATGGAATTGTCATGGGACTGATTCCCTACGACTGTCAACGTGCAAGGCATTATCCGTTTCCTGTCTTGGTCAAACTTTATGCTAAGTTTGGGCTTCATCGTTACAATATGTTGAAGGGGACAAGCTTCCAGCTCGCTACCCTAATGAAATTCAACATGTTACCGAACTATATTTCCTCTTTCTACATGACTCTGCTTGCACATGATCCCGATCCAGCTGCTGGCTCATCGCAGAAAACCTTTCAGGTTCGAGTTGATGAACAACAATTTGGCAGTTTGGATATAAACTGCTCCATTGCTAGACCTAAACACGAAGGTGACTTACTTGAAGTGTCCACCGAGACGCCCTTCATGCCTCACTTTCATGGTGGCGCACTGGGCGATGGTATCTTCAAAGTTGAATTGCCTGATTGTCTCTCAGATACTGCTTTGAATGAACTGGCCGGTGCTGTCCTCAGAGGTGAATTGCCTGAGCATGTCTTTGATGATGCTTTGTATGCACGGGCCGGTGGTATCTTCCAAGGTGAATTGCCTGATTGGCCCTCAGATGATGTTTTGAATGACGGAAAACGATTTTACATGGTGAAGGAATCAGAGTGGCAAGCCACTGATTGGATTTCTATGTATTTGGAACTTGTAATTACTACAACTGATAAGTCGATCAGTATCGTAAGTTTTACATATATATATATCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

272

Amino Acids

30.54

Weight (kDa)

4.77

Isoelectric Point (pI)

34.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 341, 732
AciI CCGC 2 cut(s) 26, 68
AclWI GGATC 2 cut(s) 320, 326
AcoI YGGCCR 1 cut(s) 561
AcsI RAATTY 2 cut(s) 16, 269
AcyI GRCGYC 2 cut(s) 61, 464
AfaI GTAC 2 cut(s) 45, 118
AfiI CCNNNNNNNGG 2 cut(s) 341, 732
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 7 cut(s) 235, 274, 449, 514, 521, 553, 679
AjnI CCWGG 1 cut(s) 112
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AleI CACNNNNGTG 1 cut(s) 435
AloI GAACNNNNNNTCC 2 cut(s) 278, 310
AluBI AGCT 3 cut(s) 246, 253, 338
AluI AGCT 3 cut(s) 246, 253, 338
Alw26I GTCTC 3 cut(s) 45, 455, 539
AlwI GGATC 2 cut(s) 320, 326
AlwNI CAGNNNCTG 2 cut(s) 341, 545
AoxI GGCC 3 cut(s) 561, 624, 659
ApeKI GCWGC 1 cut(s) 338
ApoI RAATTY 2 cut(s) 16, 269
AspLEI GCGC 1 cut(s) 494
AspS9I GGNCC 2 cut(s) 624, 660
AsuHPI GGTGA 4 cut(s) 449, 593, 656, 715
BarI GAAGNNNNNNTAC 2 cut(s) 426, 458
BbvI GCAGC 1 cut(s) 325
BccI CCATC 2 cut(s) 74, 497
BceAI ACGGC 1 cut(s) 39
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BciT130I CCWGG 1 cut(s) 114
BcoDI GTCTC 3 cut(s) 45, 455, 539
BfaI CTAG 1 cut(s) 420
BglI GCCNNNNNGGC 1 cut(s) 658
BisI GCNGC 2 cut(s) 69, 339
BlsI GCNGC 2 cut(s) 70, 340
Bme1390I CCNGG 1 cut(s) 114
BmgT120I GGNCC 2 cut(s) 624, 660
BmrFI CCNGG 1 cut(s) 114
BmrI ACTGGG 1 cut(s) 506
BmsI GCATC 1 cut(s) 598
BmuI ACTGGG 1 cut(s) 506
BplI GAGNNNNNCTC 2 cut(s) 86, 118
Bpu10I CCTNAGC 1 cut(s) 591
BsaBI GATNNNNATC 2 cut(s) 330, 785
BsaHI GRCGYC 2 cut(s) 61, 464
BsaJI CCNNGG 1 cut(s) 640
Bsc4I CCNNNNNNNGG 2 cut(s) 341, 732
Bse118I RCCGGY 2 cut(s) 563, 626
Bse1I ACTGG 2 cut(s) 501, 564
Bse3DI GCAATG 1 cut(s) 414
Bse8I GATNNNNATC 2 cut(s) 330, 785
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 640
BseJI GATNNNNATC 2 cut(s) 330, 785
BseLI CCNNNNNNNGG 2 cut(s) 341, 732
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 4 cut(s) 552, 582, 589, 678
BseNI ACTGG 2 cut(s) 501, 564
BseRI GAGGAG 1 cut(s) 44
BseXI GCAGC 1 cut(s) 325
BshFI GGCC 3 cut(s) 563, 626, 661
BsiSI CCGG 2 cut(s) 564, 627
BslFI GGGAC 2 cut(s) 150, 253
BslI CCNNNNNNNGG 2 cut(s) 341, 732
BsmAI GTCTC 3 cut(s) 45, 455, 539
BsmBI CGTCTC 1 cut(s) 455
BsmFI GGGAC 2 cut(s) 150, 253
BsnI GGCC 3 cut(s) 563, 626, 661
Bsp1407I TGTACA 1 cut(s) 43
Bsp143I GATC 3 cut(s) 325, 331, 780
BspACI CCGC 2 cut(s) 26, 68
BspANI GGCC 3 cut(s) 563, 626, 661
BspCNI CTCAG 4 cut(s) 551, 583, 588, 677
BspPI GGATC 2 cut(s) 320, 326
BsrDI GCAATG 1 cut(s) 414
BsrFI RCCGGY 2 cut(s) 563, 626
BsrGI TGTACA 1 cut(s) 43
BsrI ACTGG 2 cut(s) 501, 564
BssAI RCCGGY 2 cut(s) 563, 626
BssECI CCNNGG 1 cut(s) 640
BssMI GATC 3 cut(s) 325, 331, 780
BssNI GRCGYC 2 cut(s) 61, 464
BssT1I CCWWGG 1 cut(s) 640
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 1 cut(s) 155
BstACI GRCGYC 2 cut(s) 61, 464
BstAUI TGTACA 1 cut(s) 43
BstC8I GCNNGC 4 cut(s) 255, 318, 343, 723
BstDEI CTNAG 5 cut(s) 204, 538, 575, 591, 664
BstHHI GCGC 1 cut(s) 494
BstKTI GATC 3 cut(s) 328, 334, 783
BstMAI GTCTC 3 cut(s) 45, 455, 539
BstMBI GATC 3 cut(s) 325, 331, 780
BstMWI GCNNNNNNNGC 4 cut(s) 472, 498, 617, 658
BstNI CCWGG 1 cut(s) 114
BstNSI RCATGY 2 cut(s) 280, 599
BstSCI CCNGG 1 cut(s) 112
BstV1I GCAGC 1 cut(s) 325
BsuRI GGCC 3 cut(s) 563, 626, 661
BtgZI GCGATG 2 cut(s) 333, 516
BtsIMutI CAGTG 2 cut(s) 494, 726
Cac8I GCNNGC 4 cut(s) 255, 318, 343, 723
CaiI CAGNNNCTG 2 cut(s) 341, 545
CfoI GCGC 1 cut(s) 494
Cfr10I RCCGGY 2 cut(s) 563, 626
Cfr13I GGNCC 2 cut(s) 624, 660
CseI GACGC 2 cut(s) 50, 472
CsiI ACCWGGT 1 cut(s) 112
Csp6I GTAC 2 cut(s) 44, 117
CviAII CATG 8 cut(s) 133, 277, 307, 323, 472, 485, 596, 700
CviJI RGCY 9 cut(s) 214, 246, 253, 338, 345, 563, 626, 661, 725
CviKI_1 RGCY 9 cut(s) 214, 246, 253, 338, 345, 563, 626, 661, 725
CviQI GTAC 2 cut(s) 44, 117
DdeI CTNAG 5 cut(s) 204, 538, 575, 591, 664
DpnI GATC 3 cut(s) 327, 333, 782
DpnII GATC 3 cut(s) 325, 331, 780
EaeI YGGCCR 1 cut(s) 561
EciI GGCGGA 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 640
EcoRI GAATTC 1 cut(s) 16
EcoRII CCWGG 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 640
ErhI CCWWGG 1 cut(s) 640
Esp3I CGTCTC 1 cut(s) 455
FaeI CATG 8 cut(s) 136, 280, 310, 326, 475, 488, 599, 703
FaqI GGGAC 2 cut(s) 150, 253
FatI CATG 8 cut(s) 132, 276, 306, 322, 471, 484, 595, 699
Fnu4HI GCNGC 2 cut(s) 69, 339
Fsp4HI GCNGC 2 cut(s) 69, 339
FspBI CTAG 1 cut(s) 420
GlaI GCGC 1 cut(s) 493
GluI GCNGC 2 cut(s) 69, 339
HaeIII GGCC 3 cut(s) 563, 626, 661
HapII CCGG 2 cut(s) 564, 627
HgaI GACGC 2 cut(s) 50, 472
HhaI GCGC 1 cut(s) 494
Hin1I GRCGYC 2 cut(s) 61, 464
Hin1II CATG 8 cut(s) 136, 280, 310, 326, 475, 488, 599, 703
Hin6I GCGC 1 cut(s) 492
HinP1I GCGC 1 cut(s) 492
HincII GTYRAC 1 cut(s) 158
HindII GTYRAC 1 cut(s) 158
HindIII AAGCTT 1 cut(s) 244
HinfI GANTC 3 cut(s) 142, 310, 710
HpaII CCGG 2 cut(s) 564, 627
HphI GGTGA 4 cut(s) 449, 593, 656, 715
Hpy166II GTNNAC 3 cut(s) 44, 158, 456
Hpy188I TCNGA 5 cut(s) 79, 541, 578, 667, 715
Hpy188III TCNNGA 1 cut(s) 329
Hpy8I GTNNAC 3 cut(s) 44, 158, 456
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 4 cut(s) 229, 428, 478, 700
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 3 cut(s) 164, 320, 620
HpyF10VI GCNNNNNNNGC 4 cut(s) 472, 498, 617, 658
HpyF3I CTNAG 5 cut(s) 204, 538, 575, 591, 664
HpySE526I ACGT 1 cut(s) 160
Hsp92I GRCGYC 2 cut(s) 61, 464
Hsp92II CATG 8 cut(s) 136, 280, 310, 326, 475, 488, 599, 703
HspAI GCGC 1 cut(s) 492
Kzo9I GATC 3 cut(s) 325, 331, 780
LmnI GCTCC 1 cut(s) 416
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 1 cut(s) 598
MabI ACCWGGT 1 cut(s) 112
MaeI CTAG 1 cut(s) 420
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 3 cut(s) 221, 279, 437
MalI GATC 3 cut(s) 327, 333, 782
MboI GATC 3 cut(s) 325, 331, 780
MboII GAAGA 2 cut(s) 502, 628
MluCI AATT 9 cut(s) 16, 126, 269, 386, 521, 584, 647, 759, 814
MlyI GAGTC 1 cut(s) 304
MnlI CCTC 8 cut(s) 4, 22, 25, 307, 486, 572, 584, 673
MseI TTAA 1 cut(s) 817
MslI CAYNNNNRTG 2 cut(s) 435, 483
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 2 cut(s) 564, 627
MspR9I CCNGG 1 cut(s) 114
MvaI CCWGG 1 cut(s) 114
MwoI GCNNNNNNNGC 4 cut(s) 472, 498, 617, 658
NdeII GATC 3 cut(s) 325, 331, 780
NlaIII CATG 8 cut(s) 136, 280, 310, 326, 475, 488, 599, 703
NmuCI GTSAC 1 cut(s) 437
NspI RCATGY 2 cut(s) 280, 599
OliI CACNNNNGTG 1 cut(s) 435
PciI ACATGT 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PfeI GAWTC 2 cut(s) 142, 710
PflMI CCANNNNNTGG 2 cut(s) 341, 732
PkrI GCNGC 2 cut(s) 70, 340
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PscI ACATGT 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspPI GGNCC 2 cut(s) 624, 660
PstNI CAGNNNCTG 2 cut(s) 341, 545
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 2 cut(s) 45, 118
RsaNI GTAC 2 cut(s) 44, 117
RseI CAYNNNNRTG 2 cut(s) 435, 483
SaqAI TTAA 1 cut(s) 817
SatI GCNGC 2 cut(s) 69, 339
Sau3AI GATC 3 cut(s) 325, 331, 780
Sau96I GGNCC 2 cut(s) 624, 660
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 1 cut(s) 114
SexAI ACCWGGT 1 cut(s) 112
SfaNI GCATC 1 cut(s) 598
SmiMI CAYNNNNRTG 2 cut(s) 435, 483
Sse9I AATT 9 cut(s) 16, 126, 269, 386, 521, 584, 647, 759, 814
SsiI CCGC 2 cut(s) 26, 68
SspMI CTAG 1 cut(s) 420
StyD4I CCNGG 1 cut(s) 112
StyI CCWWGG 1 cut(s) 640
TaaI ACNGT 1 cut(s) 155
TaiI ACGT 1 cut(s) 163
TaqI TCGA 3 cut(s) 87, 370, 779
TasI AATT 9 cut(s) 16, 126, 269, 386, 521, 584, 647, 759, 814
TatI WGTACW 1 cut(s) 43
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 2 cut(s) 142, 710
Tru1I TTAA 1 cut(s) 817
Tru9I TTAA 1 cut(s) 817
TscAI CASTG 2 cut(s) 501, 733
TseFI GTSAC 1 cut(s) 437
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 437
TspDTI ATGAA 6 cut(s) 206, 281, 393, 460, 473, 570
TspGWI ACGGA 3 cut(s) 23, 165, 699
TspRI CASTG 2 cut(s) 501, 733
Van91I CCANNNNNTGG 2 cut(s) 341, 732
XapI RAATTY 2 cut(s) 16, 269
XceI RCATGY 2 cut(s) 280, 599
XspI CTAG 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.