AT1G23970

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001976
Physical Location & Seq
Reverse (-)
8481997 .. 8483875
1879 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G23970.1

Sequence Viewer

Length: 1122 bp
ATGACTACGGAGGAGGAGTCCCTTTCTGCGGCGGAGGAGGGTTACAATGTACAGAGAGACTATTGGCGTCAAGCGGAGGAATCCGATGGTTTCGATATAGAGAACATTAAACTACCACCAGGTATGCATGGACGGGTCATGGGACTGATTACCTATAACTGTCAACTTTGTTACCGTTATCCGTTTCCTGTCTTGGTCAAACTTTATGCTAAGTTTGGGCTTCATCGTTACAATTTGATCAAGGGAACAAGCTTCGAGCTCGCTGCCTTAATGAAGTTCAACATGTTACAAAACTATATGTCCTCTTTCTACATGACTCTGCTTGCACATGATCCCGATCCAGCTGCTAGCTCATCGCAGAAAACCTTTCAGGTTCGAGTTGATGAACAACAATTTGGCACTTTGGGTATAAACTGCTCCATTGCTAGACCTAAACACGAAGTGTCCACCAAGACGCCCTTCATACCTCACTTTCATGGTGGCGCATTGGGCGATGGTATATTCAAAGGTGAATTGCCTGATTGTCTCTCAGATGATGCTTTGAATGCACTGATGGAGGCTGTCTCCAAAGATGAATTGCCTGAGCATGTCTTAGATGATGCTTTGTATGCACGGGCCGGTGGTATCTTCCAAGGTGAATTGCCTGATTGGCCCTCAGATGATGCTTTGAATGATGGAAAACGATTTTACATGCTGAAGGAATCAGAGTGGCAGGCCACTGATTGGATTTCTATGTATTTGGAACTTGTAATTACTACAACTGATAGGTCGATCACTGAGACAGTTCAGAAGCCAGAAGTTCTGTCCAAGTTGGAGATTGTGAAAGTGGCGATAGAAACTGCTACCAAAGATGAGGAGCCGTCTAATGAGAGACTCAAAGCCTACAGGGCACATTTCTACATAACGTTTAAGGGCTTGGCAGAGCCTCGAGCCCCTCGGCAGGTTTTTGAGATTGGGGAGCATGTTGAACGCCAAGCTATAGTTAGAAGAGTCATGGGCCATAGAGGAGACTTGACTCTCAAGGGTAAGCTTTGTGGTGGTCAATATATCAAAAGGCGTTCTCTGGCTCTCAAGAGTGGAGAAGAGTCCCCGAATTGTAAAAAGCAAACTCGCGTCGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

373

Amino Acids

42.22

Weight (kDa)

5.77

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
protein_MS5 PF04776 214 - 338 6.6e-38 Protein MS5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 931
AccB7I CCANNNNNTGG 1 cut(s) 723
AccII CGCG 1 cut(s) 1113
AciI CCGC 3 cut(s) 29, 32, 74
AclI AACGTT 1 cut(s) 905
AclWI GGATC 2 cut(s) 326, 332
AcuI CTGAAG 1 cut(s) 716
AcyI GRCGYC 2 cut(s) 67, 455
AdeI CACNNNGTG 1 cut(s) 442
AfaI GTAC 1 cut(s) 51
AfiI CCNNNNNNNGG 3 cut(s) 28, 723, 940
AflIII ACRYGT 1 cut(s) 282
AgsI TTSAA 5 cut(s) 280, 505, 544, 670, 968
AjnI CCWGG 1 cut(s) 118
AjuI GAANNNNNNNTTGG 4 cut(s) 378, 410, 443, 475
AluBI AGCT 6 cut(s) 252, 259, 344, 351, 977, 1030
AluI AGCT 6 cut(s) 252, 259, 344, 351, 977, 1030
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 6 cut(s) 51, 530, 568, 773, 865, 1002
AlwI GGATC 2 cut(s) 326, 332
Ama87I CYCGRG 1 cut(s) 927
AoxI GGCC 4 cut(s) 615, 650, 714, 997
ApeKI GCWGC 2 cut(s) 263, 344
AspLEI GCGC 1 cut(s) 485
AspS9I GGNCC 3 cut(s) 615, 651, 997
AsuHPI GGTGA 2 cut(s) 521, 647
AsuNHI GCTAGC 1 cut(s) 347
AvaI CYCGRG 1 cut(s) 927
BaeGI GKGCMC 1 cut(s) 892
BanII GRGCYC 2 cut(s) 261, 934
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 2 cut(s) 250, 331
BccI CCATC 4 cut(s) 80, 488, 547, 668
BceAI ACGGC 1 cut(s) 844
BcgI CGANNNNNNTGC 4 cut(s) 245, 279, 326, 360
BciT130I CCWGG 1 cut(s) 120
BclI TGATCA 1 cut(s) 237
BcoDI GTCTC 6 cut(s) 51, 530, 568, 773, 865, 1002
BfaI CTAG 3 cut(s) 348, 426, 1120
BfmI CTRYAG 2 cut(s) 883, 978
BfuAI ACCTGC 1 cut(s) 931
BglI GCCNNNNNGGC 2 cut(s) 649, 887
BisI GCNGC 3 cut(s) 30, 264, 345
BlsI GCNGC 3 cut(s) 31, 265, 346
Bme1390I CCNGG 1 cut(s) 120
BmeT110I CYCGRG 1 cut(s) 927
BmgT120I GGNCC 3 cut(s) 615, 651, 997
BmiI GGNNCC 1 cut(s) 858
BmrFI CCNGG 1 cut(s) 120
BmsI GCATC 3 cut(s) 526, 589, 652
BmtI GCTAGC 1 cut(s) 351
BplI GAGNNNNNCTC 2 cut(s) 548, 580
Bpu10I CCTNAGC 1 cut(s) 582
BpuEI CTTGAG 2 cut(s) 1004, 1055
BsaBI GATNNNNATC 1 cut(s) 336
BsaHI GRCGYC 2 cut(s) 67, 455
BsaJI CCNNGG 2 cut(s) 631, 935
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 723, 940
Bse118I RCCGGY 1 cut(s) 617
Bse3DI GCAATG 1 cut(s) 420
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 2 cut(s) 631, 935
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 3 cut(s) 28, 723, 940
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 4 cut(s) 543, 573, 669, 768
BseRI GAGGAG 5 cut(s) 26, 29, 50, 869, 1020
BseSI GKGCMC 1 cut(s) 892
BseXI GCAGC 2 cut(s) 250, 331
Bsh1236I CGCG 1 cut(s) 1113
BshFI GGCC 4 cut(s) 617, 652, 716, 999
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 927
BsiSI CCGG 1 cut(s) 618
BslFI GGGAC 3 cut(s) 4, 156, 1072
BslI CCNNNNNNNGG 3 cut(s) 28, 723, 940
BsmAI GTCTC 6 cut(s) 51, 530, 568, 773, 865, 1002
BsmFI GGGAC 3 cut(s) 4, 156, 1072
BsmI GAATGC 1 cut(s) 550
BsnI GGCC 4 cut(s) 617, 652, 716, 999
BsoBI CYCGRG 1 cut(s) 927
Bsp1286I GDGCHC 3 cut(s) 261, 892, 934
Bsp1407I TGTACA 1 cut(s) 49
Bsp143I GATC 4 cut(s) 237, 331, 337, 771
BspACI CCGC 3 cut(s) 29, 32, 74
BspANI GGCC 4 cut(s) 617, 652, 716, 999
BspCNI CTCAG 4 cut(s) 542, 574, 668, 769
BspFNI CGCG 1 cut(s) 1113
BspLI GGNNCC 1 cut(s) 858
BspMI ACCTGC 1 cut(s) 931
BspOI GCTAGC 1 cut(s) 351
BspPI GGATC 2 cut(s) 326, 332
BsrDI GCAATG 1 cut(s) 420
BsrFI RCCGGY 1 cut(s) 617
BsrGI TGTACA 1 cut(s) 49
BssAI RCCGGY 1 cut(s) 617
BssECI CCNNGG 2 cut(s) 631, 935
BssMI GATC 4 cut(s) 237, 331, 337, 771
BssNI GRCGYC 2 cut(s) 67, 455
BssT1I CCWWGG 1 cut(s) 631
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 3 cut(s) 161, 176, 784
Bst6I CTCTTC 2 cut(s) 982, 1077
BstACI GRCGYC 2 cut(s) 67, 455
BstAUI TGTACA 1 cut(s) 49
BstC8I GCNNGC 4 cut(s) 261, 324, 349, 714
BstDEI CTNAG 6 cut(s) 210, 529, 582, 592, 655, 777
BstFNI CGCG 1 cut(s) 1113
BstHHI GCGC 1 cut(s) 485
BstKTI GATC 4 cut(s) 240, 334, 340, 774
BstMAI GTCTC 6 cut(s) 51, 530, 568, 773, 865, 1002
BstMBI GATC 4 cut(s) 237, 331, 337, 771
BstMWI GCNNNNNNNGC 5 cut(s) 489, 545, 608, 649, 887
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 4 cut(s) 286, 590, 694, 965
BstSCI CCNGG 1 cut(s) 118
BstSFI CTRYAG 2 cut(s) 883, 978
BstSLI GKGCMC 1 cut(s) 892
BstUI CGCG 1 cut(s) 1113
BstV1I GCAGC 2 cut(s) 250, 331
BsuRI GGCC 4 cut(s) 617, 652, 716, 999
BtgZI GCGATG 2 cut(s) 339, 507
BtsIMutI CAGTG 3 cut(s) 548, 717, 774
BveI ACCTGC 1 cut(s) 931
Cac8I GCNNGC 4 cut(s) 261, 324, 349, 714
CfoI GCGC 1 cut(s) 485
Cfr10I RCCGGY 1 cut(s) 617
Cfr13I GGNCC 3 cut(s) 615, 651, 997
CseI GACGC 3 cut(s) 56, 463, 1102
CsiI ACCWGGT 1 cut(s) 118
Csp6I GTAC 1 cut(s) 50
CviQI GTAC 1 cut(s) 50
DdeI CTNAG 6 cut(s) 210, 529, 582, 592, 655, 777
DpnI GATC 4 cut(s) 239, 333, 339, 773
DpnII GATC 4 cut(s) 237, 331, 337, 771
DraIII CACNNNGTG 1 cut(s) 442
Eam1104I CTCTTC 2 cut(s) 982, 1077
EarI CTCTTC 2 cut(s) 982, 1077
EciI GGCGGA 1 cut(s) 47
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 631
Eco24I GRGCYC 2 cut(s) 261, 934
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 716
Eco88I CYCGRG 1 cut(s) 927
EcoICRI GAGCTC 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 118
EcoT14I CCWWGG 1 cut(s) 631
EcoT22I ATGCAT 1 cut(s) 129
EcoT38I GRGCYC 2 cut(s) 261, 934
ErhI CCWWGG 1 cut(s) 631
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FaqI GGGAC 3 cut(s) 4, 156, 1072
FbaI TGATCA 1 cut(s) 237
Fnu4HI GCNGC 3 cut(s) 30, 264, 345
FriOI GRGCYC 2 cut(s) 261, 934
Fsp4HI GCNGC 3 cut(s) 30, 264, 345
FspBI CTAG 3 cut(s) 348, 426, 1120
GlaI GCGC 1 cut(s) 484
GluI GCNGC 3 cut(s) 30, 264, 345
HaeIII GGCC 4 cut(s) 617, 652, 716, 999
HapII CCGG 1 cut(s) 618
HgaI GACGC 3 cut(s) 56, 463, 1102
HhaI GCGC 1 cut(s) 485
Hin1I GRCGYC 2 cut(s) 67, 455
Hin6I GCGC 1 cut(s) 483
HinP1I GCGC 1 cut(s) 483
HincII GTYRAC 1 cut(s) 164
HindII GTYRAC 1 cut(s) 164
HindIII AAGCTT 2 cut(s) 250, 1028
HinfI GANTC 8 cut(s) 17, 80, 316, 701, 873, 990, 1015, 1085
HpaII CCGG 1 cut(s) 618
HphI GGTGA 2 cut(s) 521, 647
Hpy166II GTNNAC 2 cut(s) 164, 447
Hpy188I TCNGA 5 cut(s) 85, 532, 658, 706, 789
Hpy188III TCNNGA 2 cut(s) 335, 1072
Hpy8I GTNNAC 2 cut(s) 164, 447
Hpy99I CGWCG 1 cut(s) 1118
HpyAV CCTTC 2 cut(s) 469, 691
HpyCH4III ACNGT 3 cut(s) 161, 176, 784
HpyCH4IV ACGT 1 cut(s) 905
HpyCH4V TGCA 4 cut(s) 127, 326, 548, 611
HpyF10VI GCNNNNNNNGC 5 cut(s) 489, 545, 608, 649, 887
HpyF3I CTNAG 6 cut(s) 210, 529, 582, 592, 655, 777
HpySE526I ACGT 1 cut(s) 905
Hsp92I GRCGYC 2 cut(s) 67, 455
HspAI GCGC 1 cut(s) 483
Ksp22I TGATCA 1 cut(s) 237
Kzo9I GATC 4 cut(s) 237, 331, 337, 771
LmnI GCTCC 3 cut(s) 422, 856, 958
Lsp1109I GCAGC 2 cut(s) 250, 331
LweI GCATC 3 cut(s) 526, 589, 652
MabI ACCWGGT 1 cut(s) 118
MaeI CTAG 3 cut(s) 348, 426, 1120
MaeII ACGT 1 cut(s) 905
MaeIII GTNAC 4 cut(s) 41, 170, 227, 285
MalI GATC 4 cut(s) 239, 333, 339, 773
MboI GATC 4 cut(s) 237, 331, 337, 771
MboII GAAGA 3 cut(s) 619, 999, 1094
MhlI GDGCHC 3 cut(s) 261, 892, 934
MluCI AATT 7 cut(s) 232, 392, 512, 575, 638, 750, 1093
MlyI GAGTC 6 cut(s) 26, 310, 867, 999, 1009, 1094
MmeI TCCRAC 1 cut(s) 792
Mph1103I ATGCAT 1 cut(s) 129
MseI TTAA 3 cut(s) 108, 269, 909
MslI CAYNNNNRTG 1 cut(s) 474
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 1 cut(s) 618
MspR9I CCNGG 1 cut(s) 120
Mva1269I GAATGC 1 cut(s) 550
MvaI CCWGG 1 cut(s) 120
MvnI CGCG 1 cut(s) 1113
MwoI GCNNNNNNNGC 5 cut(s) 489, 545, 608, 649, 887
NdeII GATC 4 cut(s) 237, 331, 337, 771
NheI GCTAGC 1 cut(s) 347
NlaIV GGNNCC 1 cut(s) 858
NmeAIII GCCGAG 1 cut(s) 916
NsiI ATGCAT 1 cut(s) 129
NspI RCATGY 4 cut(s) 286, 590, 694, 965
PaeR7I CTCGAG 1 cut(s) 927
PciI ACATGT 1 cut(s) 282
PctI GAATGC 1 cut(s) 550
PfeI GAWTC 2 cut(s) 80, 701
PflMI CCANNNNNTGG 1 cut(s) 723
PkrI GCNGC 3 cut(s) 31, 265, 346
PleI GAGTC 6 cut(s) 25, 310, 867, 998, 1009, 1093
PpsI GAGTC 6 cut(s) 25, 310, 867, 998, 1009, 1093
PscI ACATGT 1 cut(s) 282
Psp124BI GAGCTC 1 cut(s) 261
Psp1406I AACGTT 1 cut(s) 905
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 1 cut(s) 858
PspPI GGNCC 3 cut(s) 615, 651, 997
PspXI VCTCGAGB 1 cut(s) 927
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 1 cut(s) 51
RsaNI GTAC 1 cut(s) 50
RseI CAYNNNNRTG 1 cut(s) 474
SacI GAGCTC 1 cut(s) 261
SaqAI TTAA 3 cut(s) 108, 269, 909
SatI GCNGC 3 cut(s) 30, 264, 345
Sau3AI GATC 4 cut(s) 237, 331, 337, 771
Sau96I GGNCC 3 cut(s) 615, 651, 997
SchI GAGTC 6 cut(s) 26, 310, 867, 999, 1009, 1094
ScrFI CCNGG 1 cut(s) 120
SduI GDGCHC 3 cut(s) 261, 892, 934
SexAI ACCWGGT 1 cut(s) 118
SfaNI GCATC 3 cut(s) 526, 589, 652
SfcI CTRYAG 2 cut(s) 883, 978
Sfr274I CTCGAG 1 cut(s) 927
SlaI CTCGAG 1 cut(s) 927
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 3 cut(s) 927, 1019, 1070
SmoI CTYRAG 3 cut(s) 927, 1019, 1070
Sse9I AATT 7 cut(s) 232, 392, 512, 575, 638, 750, 1093
SsiI CCGC 3 cut(s) 29, 32, 74
SspMI CTAG 3 cut(s) 348, 426, 1120
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 1 cut(s) 118
StyI CCWWGG 1 cut(s) 631
TaaI ACNGT 3 cut(s) 161, 176, 784
TaiI ACGT 1 cut(s) 908
TaqI TCGA 5 cut(s) 93, 255, 376, 770, 928
TasI AATT 7 cut(s) 232, 392, 512, 575, 638, 750, 1093
TatI WGTACW 1 cut(s) 49
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 2 cut(s) 80, 701
Tru1I TTAA 3 cut(s) 108, 269, 909
Tru9I TTAA 3 cut(s) 108, 269, 909
TscAI CASTG 3 cut(s) 555, 724, 781
TseI GCWGC 2 cut(s) 263, 344
TspDTI ATGAA 6 cut(s) 212, 287, 399, 451, 464, 588
TspGWI ACGGA 2 cut(s) 23, 171
TspRI CASTG 3 cut(s) 555, 724, 781
Van91I CCANNNNNTGG 1 cut(s) 723
XceI RCATGY 4 cut(s) 286, 590, 694, 965
XhoI CTCGAG 1 cut(s) 927
XspI CTAG 3 cut(s) 348, 426, 1120
Zsp2I ATGCAT 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.