AT1G27860

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001976
Physical Location & Seq
Reverse (-)
9704558 .. 9706238
1681 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27860.1

Sequence Viewer

Length: 870 bp
ATGCCGACGGAGGATAGGGTGTGTTTTACGTGGGAGGAATATGCCCGTCATGTGAGGGAATACTGGCGTGGAGTGGCAGAATCCGATGGTTTTGATTCCGAGGATATTCGAAGTCCAGTAGTGCTTACTGGACTGTTTCACTACGACTGTCAATCTGGTCGTCATTATCCTGAACCTCTCTTGGGGACAAGTTTCGAGCTCGCTGCCCTACTGAAATTCAACAAGACAATGAACCTTACGTCTTCGTACTACATTACTCTTCTTGCACATGATCCATCATTGGAGAAAACCTTTCAGGTTCGAGTTGATGAACGAGAATATGGCAGTTTGGATTTGACAGTCGCTATTGCTAGACCTAAAAAAGATCAAAATGAAGTGTCAGATGGTGTCTTCCAAGGTCCATTGCCTGATTGGCCCTCAGAAGATGCTTTGCGTCATGATAGAAACCGATTTTACGAGCTAGAGATATCAGAGTGGCAAGCCACTGATTGGATTTCTCTTTATTTGAAACTTTTGATTTTAGCGACTGATAGAGGGATGTTTGTGCAGACTGGTCTGCCCCAGGTTCAGATTCTGGAAGTGGTGATTGAAACTGAGGAAGAAAATGAGAAGCCCCCGGATAAGAGACTCAATGCCAGAAGGGCACATGTCTATATAACTTTTACGGGCTTGCCCAAGTCCCCACGTCTTGTTGAGATTGGTGAGCATGTTGAACGCAAAGCTATCATTAGAAGAGTCATCGATGCTTCAGGATACTTGACTCTCCTTGGTAAGTTTTGGAGTGGTAAAGATACTGATCAGCAGCGTTCTATGACTCTCCCGAGTGGAGAACAAGCTGAGAGTTCTAAAAAACGACCTCGCTTAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

289

Amino Acids

33.34

Weight (kDa)

5.41

Isoelectric Point (pI)

52.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
protein_MS5 PF04776 135 - 255 4.8e-35 Protein MS5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 489
AclWI GGATC 1 cut(s) 266
AcsI RAATTY 1 cut(s) 215
AcuI CTGAAG 1 cut(s) 732
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 2 cut(s) 182, 489
AflII CTTAAG 1 cut(s) 862
AflIII ACRYGT 1 cut(s) 646
AgsI TTSAA 4 cut(s) 220, 508, 590, 713
AjiI CACGTC 1 cut(s) 686
AjnI CCWGG 1 cut(s) 561
AluBI AGCT 5 cut(s) 199, 460, 722, 836, 867
AluI AGCT 5 cut(s) 199, 460, 722, 836, 867
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 1 cut(s) 619
AlwI GGATC 1 cut(s) 266
AlwNI CAGNNNCTG 1 cut(s) 574
Ama87I CYCGRG 1 cut(s) 820
AoxI GGCC 1 cut(s) 413
ApeKI GCWGC 2 cut(s) 203, 802
ApoI RAATTY 1 cut(s) 215
AspS9I GGNCC 2 cut(s) 398, 414
AsuC2I CCSGG 1 cut(s) 617
AsuHPI GGTGA 2 cut(s) 595, 713
AsuII TTCGAA 1 cut(s) 109
AvaI CYCGRG 1 cut(s) 820
AvaII GGWCC 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 646
BanII GRGCYC 1 cut(s) 201
BbsI GAAGAC 2 cut(s) 234, 382
Bbv12I GWGCWC 1 cut(s) 201
BbvI GCAGC 2 cut(s) 190, 814
BccI CCATC 3 cut(s) 80, 283, 377
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 1 cut(s) 563
BciVI GTATCC 1 cut(s) 746
BclI TGATCA 1 cut(s) 796
BcnI CCSGG 1 cut(s) 617
BcoDI GTCTC 1 cut(s) 619
BfaI CTAG 3 cut(s) 351, 461, 868
BfrI CTTAAG 1 cut(s) 862
BfuI GTATCC 1 cut(s) 746
BglI GCCNNNNNGGC 2 cut(s) 412, 641
BisI GCNGC 2 cut(s) 204, 803
BlsI GCNGC 2 cut(s) 205, 804
Bme1390I CCNGG 2 cut(s) 563, 617
Bme18I GGWCC 1 cut(s) 398
BmeT110I CYCGRG 1 cut(s) 820
BmgBI CACGTC 1 cut(s) 686
BmgT120I GGNCC 2 cut(s) 398, 414
BmrFI CCNGG 2 cut(s) 563, 617
BmsI GCATC 2 cut(s) 415, 733
BpiI GAAGAC 2 cut(s) 234, 382
Bpu14I TTCGAA 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 617
Bsa29I ATCGAT 1 cut(s) 741
BsaAI YACGTR 1 cut(s) 30
BsaBI GATNNNNATC 1 cut(s) 795
BsaJI CCNNGG 5 cut(s) 99, 394, 561, 615, 766
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 489
Bse1I ACTGG 4 cut(s) 68, 116, 133, 556
Bse3DI GCAATG 1 cut(s) 401
Bse8I GATNNNNATC 1 cut(s) 795
BseBI CCWGG 1 cut(s) 563
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 5 cut(s) 99, 394, 561, 615, 766
BseGI GGATG 1 cut(s) 543
BseJI GATNNNNATC 1 cut(s) 795
BseLI CCNNNNNNNGG 2 cut(s) 182, 489
BseMI GCAATG 1 cut(s) 401
BseMII CTCAG 3 cut(s) 432, 585, 828
BseNI ACTGG 4 cut(s) 68, 116, 133, 556
BseSI GKGCMC 1 cut(s) 646
BseXI GCAGC 2 cut(s) 190, 814
BsgI GTGCAG 1 cut(s) 566
BshFI GGCC 1 cut(s) 415
BshVI ATCGAT 1 cut(s) 741
BsiHKAI GWGCWC 1 cut(s) 201
BsiHKCI CYCGRG 1 cut(s) 820
BsiSI CCGG 1 cut(s) 617
BslFI GGGAC 2 cut(s) 199, 664
BslI CCNNNNNNNGG 2 cut(s) 182, 489
BsmAI GTCTC 1 cut(s) 619
BsmFI GGGAC 2 cut(s) 199, 664
BsnI GGCC 1 cut(s) 415
BsoBI CYCGRG 1 cut(s) 820
Bsp119I TTCGAA 1 cut(s) 109
Bsp1286I GDGCHC 2 cut(s) 201, 646
Bsp143I GATC 3 cut(s) 271, 364, 796
BspANI GGCC 1 cut(s) 415
BspCNI CTCAG 3 cut(s) 431, 586, 829
BspDI ATCGAT 1 cut(s) 741
BspHI TCATGA 1 cut(s) 436
BspPI GGATC 1 cut(s) 266
BspT104I TTCGAA 1 cut(s) 109
BspTI CTTAAG 1 cut(s) 862
BsrDI GCAATG 1 cut(s) 401
BsrI ACTGG 4 cut(s) 68, 116, 133, 556
BssECI CCNNGG 5 cut(s) 99, 394, 561, 615, 766
BssMI GATC 3 cut(s) 271, 364, 796
BssT1I CCWWGG 2 cut(s) 394, 766
Bst2UI CCWGG 1 cut(s) 563
Bst4CI ACNGT 3 cut(s) 135, 149, 340
Bst6I CTCTTC 2 cut(s) 264, 727
BstAFI CTTAAG 1 cut(s) 862
BstBAI YACGTR 1 cut(s) 30
BstBI TTCGAA 1 cut(s) 109
BstC8I GCNNGC 3 cut(s) 201, 480, 671
BstDEI CTNAG 3 cut(s) 418, 594, 837
BstF5I GGATG 1 cut(s) 543
BstKTI GATC 3 cut(s) 274, 367, 799
BstMAI GTCTC 1 cut(s) 619
BstMBI GATC 3 cut(s) 271, 364, 796
BstMWI GCNNNNNNNGC 2 cut(s) 412, 641
BstNI CCWGG 1 cut(s) 563
BstNSI RCATGY 2 cut(s) 650, 710
BstSCI CCNGG 2 cut(s) 561, 615
BstSLI GKGCMC 1 cut(s) 646
BstV1I GCAGC 2 cut(s) 190, 814
BstV2I GAAGAC 2 cut(s) 234, 382
Bsu15I ATCGAT 1 cut(s) 741
BsuI GTATCC 1 cut(s) 746
BsuRI GGCC 1 cut(s) 415
BsuTUI ATCGAT 1 cut(s) 741
BtrI CACGTC 1 cut(s) 686
BtsCI GGATG 1 cut(s) 543
BtsIMutI CAGTG 1 cut(s) 483
Cac8I GCNNGC 3 cut(s) 201, 480, 671
CaiI CAGNNNCTG 1 cut(s) 574
CciI TCATGA 1 cut(s) 436
Cfr13I GGNCC 2 cut(s) 398, 414
ClaI ATCGAT 1 cut(s) 741
CseI GACGC 1 cut(s) 422
Csp6I GTAC 1 cut(s) 247
CviAII CATG 5 cut(s) 50, 269, 437, 647, 707
CviJI RGCY 9 cut(s) 199, 415, 460, 482, 613, 669, 722, 836, 867
CviKI_1 RGCY 9 cut(s) 199, 415, 460, 482, 613, 669, 722, 836, 867
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 3 cut(s) 418, 594, 837
DpnI GATC 3 cut(s) 273, 366, 798
DpnII GATC 3 cut(s) 271, 364, 796
Eam1104I CTCTTC 2 cut(s) 264, 727
EarI CTCTTC 2 cut(s) 264, 727
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 2 cut(s) 394, 766
Eco24I GRGCYC 1 cut(s) 201
Eco32I GATATC 1 cut(s) 468
Eco47I GGWCC 1 cut(s) 398
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 732
Eco88I CYCGRG 1 cut(s) 820
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 561
EcoRV GATATC 1 cut(s) 468
EcoT14I CCWWGG 2 cut(s) 394, 766
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 2 cut(s) 394, 766
FaeI CATG 5 cut(s) 53, 272, 440, 650, 710
FaqI GGGAC 2 cut(s) 199, 664
FatI CATG 5 cut(s) 49, 268, 436, 646, 706
FbaI TGATCA 1 cut(s) 796
Fnu4HI GCNGC 2 cut(s) 204, 803
FokI GGATG 1 cut(s) 550
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 2 cut(s) 204, 803
FspBI CTAG 3 cut(s) 351, 461, 868
GluI GCNGC 2 cut(s) 204, 803
HaeIII GGCC 1 cut(s) 415
HapII CCGG 1 cut(s) 617
HgaI GACGC 1 cut(s) 422
Hin1II CATG 5 cut(s) 53, 272, 440, 650, 710
HinfI GANTC 7 cut(s) 80, 95, 571, 627, 735, 760, 814
HpaII CCGG 1 cut(s) 617
HphI GGTGA 2 cut(s) 595, 713
Hpy188I TCNGA 6 cut(s) 85, 100, 382, 421, 472, 570
Hpy188III TCNNGA 5 cut(s) 170, 437, 575, 750, 820
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 633
HpyCH4III ACNGT 3 cut(s) 135, 149, 340
HpyCH4IV ACGT 3 cut(s) 29, 239, 685
HpyCH4V TGCA 2 cut(s) 266, 547
HpyF10VI GCNNNNNNNGC 2 cut(s) 412, 641
HpyF3I CTNAG 3 cut(s) 418, 594, 837
HpySE526I ACGT 3 cut(s) 29, 239, 685
Hsp92II CATG 5 cut(s) 53, 272, 440, 650, 710
Ksp22I TGATCA 1 cut(s) 796
Kzo9I GATC 3 cut(s) 271, 364, 796
Lsp1109I GCAGC 2 cut(s) 190, 814
LweI GCATC 2 cut(s) 415, 733
MaeI CTAG 3 cut(s) 351, 461, 868
MaeII ACGT 3 cut(s) 29, 239, 685
MalI GATC 3 cut(s) 273, 366, 798
MboI GATC 3 cut(s) 271, 364, 796
MboII GAAGA 6 cut(s) 234, 251, 382, 434, 611, 744
MhlI GDGCHC 2 cut(s) 201, 646
MluCI AATT 1 cut(s) 215
MlyI GAGTC 4 cut(s) 621, 744, 754, 808
MnlI CCTC 9 cut(s) 4, 28, 48, 94, 186, 427, 527, 589, 867
MseI TTAA 1 cut(s) 863
MspCI CTTAAG 1 cut(s) 862
MspI CCGG 1 cut(s) 617
MspR9I CCNGG 2 cut(s) 563, 617
MvaI CCWGG 1 cut(s) 563
MwoI GCNNNNNNNGC 2 cut(s) 412, 641
NciI CCSGG 1 cut(s) 617
NdeII GATC 3 cut(s) 271, 364, 796
NlaIII CATG 5 cut(s) 53, 272, 440, 650, 710
NspI RCATGY 2 cut(s) 650, 710
NspV TTCGAA 1 cut(s) 109
PagI TCATGA 1 cut(s) 436
PciI ACATGT 1 cut(s) 646
PfeI GAWTC 3 cut(s) 80, 95, 571
PflMI CCANNNNNTGG 1 cut(s) 489
PkrI GCNGC 2 cut(s) 205, 804
PleI GAGTC 4 cut(s) 621, 743, 754, 808
PpsI GAGTC 4 cut(s) 621, 743, 754, 808
Ppu21I YACGTR 1 cut(s) 30
PscI ACATGT 1 cut(s) 646
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 561
PspGI CCWGG 1 cut(s) 561
PspPI GGNCC 2 cut(s) 398, 414
PstNI CAGNNNCTG 1 cut(s) 574
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 1 cut(s) 863
SatI GCNGC 2 cut(s) 204, 803
Sau3AI GATC 3 cut(s) 271, 364, 796
Sau96I GGNCC 2 cut(s) 398, 414
SchI GAGTC 4 cut(s) 621, 744, 754, 808
ScrFI CCNGG 2 cut(s) 563, 617
SduI GDGCHC 2 cut(s) 201, 646
SfaNI GCATC 2 cut(s) 415, 733
SfuI TTCGAA 1 cut(s) 109
SinI GGWCC 1 cut(s) 398
SmlI CTYRAG 1 cut(s) 862
SmoI CTYRAG 1 cut(s) 862
Sse9I AATT 1 cut(s) 215
SspMI CTAG 3 cut(s) 351, 461, 868
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 2 cut(s) 561, 615
StyI CCWWGG 2 cut(s) 394, 766
TaaI ACNGT 3 cut(s) 135, 149, 340
TaiI ACGT 3 cut(s) 32, 242, 688
TaqI TCGA 4 cut(s) 109, 195, 301, 741
TasI AATT 1 cut(s) 215
TfiI GAWTC 3 cut(s) 80, 95, 571
Tru1I TTAA 1 cut(s) 863
Tru9I TTAA 1 cut(s) 863
TscAI CASTG 1 cut(s) 490
TseI GCWGC 2 cut(s) 203, 802
TspDTI ATGAA 3 cut(s) 245, 324, 387
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 1 cut(s) 490
Van91I CCANNNNNTGG 1 cut(s) 489
Vha464I CTTAAG 1 cut(s) 862
VpaK11BI GGWCC 1 cut(s) 398
XapI RAATTY 1 cut(s) 215
XceI RCATGY 2 cut(s) 650, 710
XcmI CCANNNNNNNNNTGG 1 cut(s) 408
XspI CTAG 3 cut(s) 351, 461, 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.