AT2G20625

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001976
Physical Location & Seq
Reverse (-)
8896154 .. 8897218
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G20625.1

Sequence Viewer

Length: 540 bp
ATGGTTGTTGAAGCTTATGTAAAGCAGACTGAAGCTCTTGAGAAGAAGAATAGAATAGTGGAACTGATGTTGGAGCGGGAGCATGCATCTAGCGTTAAGAGTGTACTAGAGACGCTTAATGGATTGCCTGGAGTTAGGATGTGGTCTCCATTTCACAAGACATCAATTGACCATCTTATAGCTGATGAAGCGAGTCGGCAGGGTTTTATAGCGTTTCCTAGGGCTGAACATAAGAGAACTGGAGAAGAAGATTTGATATGGGATCCAAATGCTGTTGATGACTTTTGTAGAGGTGATATGCATAAGTGGCTTGAGGGTGAAGGAATCGGGTTTGCGAGACAAAGAATGGCTTTATTTATATATGCTCTCCAAAGAGCTTATTTACCATTTGAGATGAAGAAAGTAGTAGTGCAAACAAGGGAAGATATAGAGTCGAGTATGAAACTGAAGGCCAGCAATGCAATCTTCTACATGAATTTCAAGGCATATGGAGGTCCACAATGCAGAGGAATAGTAAGGAAACAAGTGATTGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

179

Amino Acids

20.62

Weight (kDa)

6.98

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
protein_MS5 PF04776 127 - 176 2.2e-10 Protein MS5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 76
AciI CCGC 1 cut(s) 76
AclWI GGATC 2 cut(s) 257, 270
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 2 cut(s) 51, 467
AfaI GTAC 1 cut(s) 105
AgsI TTSAA 3 cut(s) 11, 481, 533
AjnI CCWGG 1 cut(s) 127
AluBI AGCT 4 cut(s) 14, 35, 182, 377
AluI AGCT 4 cut(s) 14, 35, 182, 377
Alw26I GTCTC 3 cut(s) 104, 150, 331
AlwI GGATC 2 cut(s) 257, 270
AoxI GGCC 1 cut(s) 450
ApoI RAATTY 1 cut(s) 475
AspA2I CCTAGG 1 cut(s) 218
AspS9I GGNCC 1 cut(s) 494
AsuHPI GGTGA 2 cut(s) 305, 329
AvaII GGWCC 1 cut(s) 494
AvrII CCTAGG 1 cut(s) 218
BamHI GGATCC 1 cut(s) 262
BccI CCATC 1 cut(s) 180
BciT130I CCWGG 1 cut(s) 129
BcoDI GTCTC 3 cut(s) 104, 150, 331
BfaI CTAG 4 cut(s) 90, 107, 219, 538
BlnI CCTAGG 1 cut(s) 218
Bme1390I CCNGG 1 cut(s) 129
Bme18I GGWCC 1 cut(s) 494
BmgT120I GGNCC 1 cut(s) 494
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 1 cut(s) 129
BmsI GCATC 1 cut(s) 95
BpmI CTGGAG 2 cut(s) 150, 261
BpuEI CTTGAG 2 cut(s) 59, 332
BsaI GGTCTC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 218
Bse1I ACTGG 1 cut(s) 244
Bse3DI GCAATG 1 cut(s) 463
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 218
BseGI GGATG 1 cut(s) 144
BseMI GCAATG 1 cut(s) 463
BseNI ACTGG 1 cut(s) 244
BshFI GGCC 1 cut(s) 452
BsmAI GTCTC 3 cut(s) 104, 150, 331
BsmBI CGTCTC 1 cut(s) 104
BsnI GGCC 1 cut(s) 452
Bso31I GGTCTC 1 cut(s) 150
Bsp143I GATC 1 cut(s) 262
BspACI CCGC 1 cut(s) 76
BspANI GGCC 1 cut(s) 452
BspLI GGNNCC 1 cut(s) 264
BspPI GGATC 2 cut(s) 257, 270
BspTNI GGTCTC 1 cut(s) 150
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 463
BsrI ACTGG 1 cut(s) 244
BssECI CCNNGG 1 cut(s) 218
BssMI GATC 1 cut(s) 262
BssT1I CCWWGG 1 cut(s) 218
Bst2UI CCWGG 1 cut(s) 129
BstC8I GCNNGC 2 cut(s) 84, 454
BstF5I GGATG 1 cut(s) 144
BstKTI GATC 1 cut(s) 265
BstMAI GTCTC 3 cut(s) 104, 150, 331
BstMBI GATC 1 cut(s) 262
BstMWI GCNNNNNNNGC 3 cut(s) 188, 307, 458
BstNI CCWGG 1 cut(s) 129
BstNSI RCATGY 1 cut(s) 86
BstSCI CCNGG 1 cut(s) 127
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BsuRI GGCC 1 cut(s) 452
BtsCI GGATG 1 cut(s) 144
Cac8I GCNNGC 2 cut(s) 84, 454
Cfr13I GGNCC 1 cut(s) 494
CseI GACGC 1 cut(s) 121
Csp6I GTAC 1 cut(s) 104
CviAII CATG 2 cut(s) 83, 472
CviJI RGCY 8 cut(s) 14, 35, 182, 224, 310, 350, 377, 452
CviKI_1 RGCY 8 cut(s) 14, 35, 182, 224, 310, 350, 377, 452
CviQI GTAC 1 cut(s) 104
DpnI GATC 1 cut(s) 264
DpnII GATC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 218
Eco31I GGTCTC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 494
Eco57I CTGAAG 2 cut(s) 51, 467
EcoRII CCWGG 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 218
EcoT22I ATGCAT 2 cut(s) 88, 303
ErhI CCWWGG 1 cut(s) 218
Esp3I CGTCTC 1 cut(s) 104
FaeI CATG 2 cut(s) 86, 475
FalI AAGNNNNNCTT 2 cut(s) 334, 366
FatI CATG 2 cut(s) 82, 471
FauI CCCGC 1 cut(s) 69
FauNDI CATATG 1 cut(s) 487
FokI GGATG 1 cut(s) 151
FspBI CTAG 4 cut(s) 90, 107, 219, 538
GsuI CTGGAG 2 cut(s) 150, 261
HaeIII GGCC 1 cut(s) 452
HgaI GACGC 1 cut(s) 121
Hin1II CATG 2 cut(s) 86, 475
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 3 cut(s) 193, 324, 431
HphI GGTGA 2 cut(s) 305, 329
Hpy166II GTNNAC 2 cut(s) 104, 497
Hpy188III TCNNGA 1 cut(s) 38
Hpy8I GTNNAC 2 cut(s) 104, 497
HpyAV CCTTC 2 cut(s) 314, 442
HpyCH4V TGCA 5 cut(s) 86, 301, 412, 461, 504
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 307, 458
Hsp92II CATG 2 cut(s) 86, 475
Kzo9I GATC 1 cut(s) 262
LmnI GCTCC 2 cut(s) 73, 79
LpnPI CCDG 5 cut(s) 114, 141, 185, 225, 466
LweI GCATC 1 cut(s) 95
MaeI CTAG 4 cut(s) 90, 107, 219, 538
MalI GATC 1 cut(s) 264
MbiI CCGCTC 1 cut(s) 76
MboI GATC 1 cut(s) 262
MboII GAAGA 7 cut(s) 55, 58, 257, 260, 409, 434, 457
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 1 cut(s) 262
MluCI AATT 2 cut(s) 165, 475
MlyI GAGTC 2 cut(s) 202, 440
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 4 cut(s) 284, 307, 485, 500
Mph1103I ATGCAT 2 cut(s) 88, 303
MseI TTAA 2 cut(s) 96, 117
MspR9I CCNGG 1 cut(s) 129
MunI CAATTG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 3 cut(s) 188, 307, 458
NdeI CATATG 1 cut(s) 487
NdeII GATC 1 cut(s) 262
NlaIII CATG 2 cut(s) 86, 475
NlaIV GGNNCC 1 cut(s) 264
NsiI ATGCAT 2 cut(s) 88, 303
NspI RCATGY 1 cut(s) 86
PaeI GCATGC 1 cut(s) 86
PfeI GAWTC 1 cut(s) 324
PleI GAGTC 2 cut(s) 201, 439
PpsI GAGTC 2 cut(s) 201, 439
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 1 cut(s) 264
PspPI GGNCC 1 cut(s) 494
PsuI RGATCY 1 cut(s) 262
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
SaqAI TTAA 2 cut(s) 96, 117
Sau3AI GATC 1 cut(s) 262
Sau96I GGNCC 1 cut(s) 494
SchI GAGTC 2 cut(s) 202, 440
ScrFI CCNGG 1 cut(s) 129
SetI ASST 6 cut(s) 16, 37, 184, 295, 379, 496
SfaNI GCATC 1 cut(s) 95
SinI GGWCC 1 cut(s) 494
SmlI CTYRAG 2 cut(s) 38, 311
SmoI CTYRAG 2 cut(s) 38, 311
SphI GCATGC 1 cut(s) 86
Sse9I AATT 2 cut(s) 165, 475
SsiI CCGC 1 cut(s) 76
SspMI CTAG 4 cut(s) 90, 107, 219, 538
StyD4I CCNGG 1 cut(s) 127
StyI CCWWGG 1 cut(s) 218
TaqI TCGA 1 cut(s) 434
TasI AATT 2 cut(s) 165, 475
TatI WGTACW 1 cut(s) 103
TfiI GAWTC 1 cut(s) 324
Tru1I TTAA 2 cut(s) 96, 117
Tru9I TTAA 2 cut(s) 96, 117
TspDTI ATGAA 4 cut(s) 201, 410, 455, 488
VpaK11BI GGWCC 1 cut(s) 494
XapI RAATTY 1 cut(s) 475
XceI RCATGY 1 cut(s) 86
XmaJI CCTAGG 1 cut(s) 218
XspI CTAG 4 cut(s) 90, 107, 219, 538
Zsp2I ATGCAT 2 cut(s) 88, 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.