AT1G28500

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001976
Physical Location & Seq
Forward (+)
10018691 .. 10020479
1789 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G28500.1

Sequence Viewer

Length: 981 bp
ATGTCGACGGAGGATGCTCTGTGTTTTACGGGGGAGGAATATGCCCGTCATGTGAGGGAGTACTGGCGTGGAGTGGCGGAATCCGATGGTTTTGATTCCGAGGATATTCGAAGTCCAGTAGTGCTTACTGGACTGTTTCACTACGACTGTCAATCTGGTCGTCGTTATCCTGACCCTCTCTTGGTCAAGCGATATGCTTTGTTGGGGCTTCATCGTTTCAATATTTTGCAGGGGACAAGTTTCGAGCTCGATGCCCTACAGAAATTCAACAAGACAATGAACCTTACGTCTTCGTACTACATTACTTTGCTTGCAAACGAGCCAGGAGCTATTCCTTTGCAGAAAACCTTTCAGGTTCGAGTTGATGAACGAAAATATGACACTTTGGATTTGACAGTCGCTATTGCTAGGCTTAAAAAAGATCAAAATGAAGCGGCGGAGACCACCAAGGAGCCCTTCGTACCTCACTTTTGTTGTAGCGCAGTGTCAGATGGTGTCTTCCAAGGTCCATTGCCTGATTGGCCCTCAGATGATGCTTTGCGTCATGATAGAAACCGATTTTACGAGCTAGAGAAATCAGAGTGGCAAGCCACTGATTGGATTTCTCTTTATTTGGAACTTTTGATTTTAGCAACTGATAGAGGAATGTTTGGTGTAGCGCAGACTGGTCTGCCTCAGGTTCAGATTCTGAAAGTGGTGATTGAAACTGAGGAAGAAAATGAGAAGCCCCTGGATAAGAGACTCAATGCCAGAAGGGCACATGTCTATATAACTTTTACGGGCTTGCCCAAGTCCCCACGTCTTGTTGAGATTGGTGAGCATGTTGAACGCAAAGCTATCATTAGAAGAGTCATCGATGATTCAGGATACTTGACTCTCCTTGGTAAGTTTTGGAGTGGTAAAGATACCGAGCAGCGTTCTAAGACTCGCCAGAGTGAAGAAAAAGTCGAGAGTTCTCAAAAACGATCTCGCTTATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

326

Amino Acids

37.52

Weight (kDa)

5.9

Isoelectric Point (pI)

56.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
protein_MS5 PF04776 171 - 293 1e-35 Protein MS5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 597
AccI GTMKAC 1 cut(s) 5
AciI CCGC 3 cut(s) 77, 434, 437
AcsI RAATTY 1 cut(s) 263
AfaI GTAC 3 cut(s) 62, 296, 462
AfiI CCNNNNNNNGG 2 cut(s) 181, 597
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 4 cut(s) 220, 268, 704, 827
AjiI CACGTC 1 cut(s) 800
AjnI CCWGG 2 cut(s) 322, 729
AjuI GAANNNNNNNTTGG 2 cut(s) 440, 472
AluBI AGCT 4 cut(s) 247, 329, 568, 836
AluI AGCT 4 cut(s) 247, 329, 568, 836
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 2 cut(s) 434, 733
AlwNI CAGNNNCTG 1 cut(s) 688
AoxI GGCC 1 cut(s) 521
ApeKI GCWGC 1 cut(s) 913
ApoI RAATTY 1 cut(s) 263
AspLEI GCGC 2 cut(s) 482, 661
AspS9I GGNCC 2 cut(s) 506, 522
AsuHPI GGTGA 2 cut(s) 709, 827
AsuII TTCGAA 1 cut(s) 109
AvaII GGWCC 1 cut(s) 506
AxyI CCTNAGG 1 cut(s) 675
BaeGI GKGCMC 1 cut(s) 760
BanII GRGCYC 2 cut(s) 249, 456
BbsI GAAGAC 2 cut(s) 282, 490
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 925
BccI CCATC 2 cut(s) 80, 485
BcgI CGANNNNNNTGC 2 cut(s) 233, 267
BciT130I CCWGG 2 cut(s) 324, 731
BciVI GTATCC 1 cut(s) 860
BcoDI GTCTC 2 cut(s) 434, 733
BfaI CTAG 3 cut(s) 408, 569, 979
BfmI CTRYAG 1 cut(s) 257
BfuI GTATCC 1 cut(s) 860
BglI GCCNNNNNGGC 2 cut(s) 520, 755
BisI GCNGC 2 cut(s) 435, 914
BlsI GCNGC 2 cut(s) 436, 915
BmcAI AGTACT 1 cut(s) 62
Bme1390I CCNGG 2 cut(s) 324, 731
Bme18I GGWCC 1 cut(s) 506
BmgBI CACGTC 1 cut(s) 800
BmgT120I GGNCC 2 cut(s) 506, 522
BmiI GGNNCC 1 cut(s) 453
BmrFI CCNGG 2 cut(s) 324, 731
BmsI GCATC 3 cut(s) 4, 241, 523
BpiI GAAGAC 2 cut(s) 282, 490
Bpu14I TTCGAA 1 cut(s) 109
Bsa29I ATCGAT 1 cut(s) 855
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 5 cut(s) 99, 447, 502, 729, 880
Bsc4I CCNNNNNNNGG 2 cut(s) 181, 597
Bse1I ACTGG 4 cut(s) 68, 116, 133, 670
Bse21I CCTNAGG 1 cut(s) 675
Bse3DI GCAATG 1 cut(s) 509
BseBI CCWGG 2 cut(s) 324, 731
BseCI ATCGAT 1 cut(s) 855
BseDI CCNNGG 5 cut(s) 99, 447, 502, 729, 880
BseGI GGATG 1 cut(s) 19
BseLI CCNNNNNNNGG 2 cut(s) 181, 597
BseMI GCAATG 1 cut(s) 509
BseMII CTCAG 3 cut(s) 540, 689, 699
BseNI ACTGG 4 cut(s) 68, 116, 133, 670
BseSI GKGCMC 1 cut(s) 760
BseXI GCAGC 1 cut(s) 925
BshFI GGCC 1 cut(s) 523
BshVI ATCGAT 1 cut(s) 855
BsiHKAI GWGCWC 1 cut(s) 249
BslFI GGGAC 2 cut(s) 247, 778
BslI CCNNNNNNNGG 2 cut(s) 181, 597
BsmAI GTCTC 2 cut(s) 434, 733
BsmFI GGGAC 2 cut(s) 247, 778
BsnI GGCC 1 cut(s) 523
Bso31I GGTCTC 1 cut(s) 434
Bsp119I TTCGAA 1 cut(s) 109
Bsp1286I GDGCHC 3 cut(s) 249, 456, 760
Bsp143I GATC 2 cut(s) 421, 965
BspACI CCGC 3 cut(s) 77, 434, 437
BspANI GGCC 1 cut(s) 523
BspCNI CTCAG 3 cut(s) 539, 688, 700
BspDI ATCGAT 1 cut(s) 855
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 1 cut(s) 453
BspT104I TTCGAA 1 cut(s) 109
BspTNI GGTCTC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 509
BsrI ACTGG 4 cut(s) 68, 116, 133, 670
BssECI CCNNGG 5 cut(s) 99, 447, 502, 729, 880
BssMI GATC 2 cut(s) 421, 965
BssT1I CCWWGG 3 cut(s) 447, 502, 880
Bst2UI CCWGG 2 cut(s) 324, 731
Bst4CI ACNGT 3 cut(s) 135, 149, 397
Bst6I CTCTTC 1 cut(s) 841
BstBI TTCGAA 1 cut(s) 109
BstC8I GCNNGC 3 cut(s) 312, 588, 785
BstDEI CTNAG 4 cut(s) 526, 675, 708, 921
BstF5I GGATG 1 cut(s) 19
BstHHI GCGC 2 cut(s) 482, 661
BstKTI GATC 2 cut(s) 424, 968
BstMAI GTCTC 2 cut(s) 434, 733
BstMBI GATC 2 cut(s) 421, 965
BstMWI GCNNNNNNNGC 2 cut(s) 520, 755
BstNI CCWGG 2 cut(s) 324, 731
BstNSI RCATGY 2 cut(s) 764, 824
BstSCI CCNGG 2 cut(s) 322, 729
BstSFI CTRYAG 1 cut(s) 257
BstSLI GKGCMC 1 cut(s) 760
BstV1I GCAGC 1 cut(s) 925
BstV2I GAAGAC 2 cut(s) 282, 490
Bsu15I ATCGAT 1 cut(s) 855
Bsu36I CCTNAGG 1 cut(s) 675
BsuI GTATCC 1 cut(s) 860
BsuRI GGCC 1 cut(s) 523
BsuTUI ATCGAT 1 cut(s) 855
BtrI CACGTC 1 cut(s) 800
BtsCI GGATG 1 cut(s) 19
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 2 cut(s) 489, 591
Cac8I GCNNGC 3 cut(s) 312, 588, 785
CaiI CAGNNNCTG 1 cut(s) 688
CciI TCATGA 1 cut(s) 544
CfoI GCGC 2 cut(s) 482, 661
Cfr13I GGNCC 2 cut(s) 506, 522
ClaI ATCGAT 1 cut(s) 855
CseI GACGC 1 cut(s) 530
Csp6I GTAC 3 cut(s) 61, 295, 461
CviAII CATG 4 cut(s) 50, 545, 761, 821
CviQI GTAC 3 cut(s) 61, 295, 461
DdeI CTNAG 4 cut(s) 526, 675, 708, 921
DpnI GATC 2 cut(s) 423, 967
DpnII GATC 2 cut(s) 421, 965
Eam1104I CTCTTC 1 cut(s) 841
EarI CTCTTC 1 cut(s) 841
EciI GGCGGA 2 cut(s) 92, 452
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 3 cut(s) 447, 502, 880
Eco24I GRGCYC 2 cut(s) 249, 456
Eco31I GGTCTC 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 506
Eco53kI GAGCTC 1 cut(s) 247
Eco81I CCTNAGG 1 cut(s) 675
EcoICRI GAGCTC 1 cut(s) 247
EcoRII CCWGG 2 cut(s) 322, 729
EcoT14I CCWWGG 3 cut(s) 447, 502, 880
EcoT38I GRGCYC 2 cut(s) 249, 456
ErhI CCWWGG 3 cut(s) 447, 502, 880
FaeI CATG 4 cut(s) 53, 548, 764, 824
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FaqI GGGAC 2 cut(s) 247, 778
FatI CATG 4 cut(s) 49, 544, 760, 820
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 435, 914
FokI GGATG 1 cut(s) 26
FriOI GRGCYC 2 cut(s) 249, 456
Fsp4HI GCNGC 2 cut(s) 435, 914
FspBI CTAG 3 cut(s) 408, 569, 979
GlaI GCGC 2 cut(s) 481, 660
GluI GCNGC 2 cut(s) 435, 914
HaeIII GGCC 1 cut(s) 523
HgaI GACGC 1 cut(s) 530
HhaI GCGC 2 cut(s) 482, 661
Hin1II CATG 4 cut(s) 53, 548, 764, 824
Hin6I GCGC 2 cut(s) 480, 659
HinP1I GCGC 2 cut(s) 480, 659
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 8 cut(s) 80, 95, 685, 741, 849, 860, 874, 925
HphI GGTGA 2 cut(s) 709, 827
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 7 cut(s) 85, 100, 490, 529, 580, 684, 690
Hpy188III TCNNGA 4 cut(s) 170, 545, 864, 949
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 2 cut(s) 10, 165
HpyAV CCTTC 2 cut(s) 466, 747
HpyCH4III ACNGT 3 cut(s) 135, 149, 397
HpyCH4IV ACGT 2 cut(s) 287, 799
HpyCH4V TGCA 3 cut(s) 229, 314, 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 520, 755
HpyF3I CTNAG 4 cut(s) 526, 675, 708, 921
HpySE526I ACGT 2 cut(s) 287, 799
Hsp92II CATG 4 cut(s) 53, 548, 764, 824
HspAI GCGC 2 cut(s) 480, 659
Kzo9I GATC 2 cut(s) 421, 965
LmnI GCTCC 2 cut(s) 326, 451
Lsp1109I GCAGC 1 cut(s) 925
LweI GCATC 3 cut(s) 4, 241, 523
MaeI CTAG 3 cut(s) 408, 569, 979
MaeII ACGT 2 cut(s) 287, 799
MalI GATC 2 cut(s) 423, 967
MboI GATC 2 cut(s) 421, 965
MboII GAAGA 5 cut(s) 282, 490, 725, 858, 950
MhlI GDGCHC 3 cut(s) 249, 456, 760
MluCI AATT 1 cut(s) 263
MlyI GAGTC 4 cut(s) 735, 858, 868, 919
MseI TTAA 1 cut(s) 414
MspR9I CCNGG 2 cut(s) 324, 731
MvaI CCWGG 2 cut(s) 324, 731
MwoI GCNNNNNNNGC 2 cut(s) 520, 755
NdeII GATC 2 cut(s) 421, 965
NlaIII CATG 4 cut(s) 53, 548, 764, 824
NlaIV GGNNCC 1 cut(s) 453
NspI RCATGY 2 cut(s) 764, 824
NspV TTCGAA 1 cut(s) 109
PagI TCATGA 1 cut(s) 544
PciI ACATGT 1 cut(s) 760
PfeI GAWTC 4 cut(s) 80, 95, 685, 860
PflMI CCANNNNNTGG 1 cut(s) 597
PkrI GCNGC 2 cut(s) 436, 915
PleI GAGTC 4 cut(s) 735, 857, 868, 919
PpsI GAGTC 4 cut(s) 735, 857, 868, 919
PscI ACATGT 1 cut(s) 760
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 2 cut(s) 322, 729
PspGI CCWGG 2 cut(s) 322, 729
PspN4I GGNNCC 1 cut(s) 453
PspPI GGNCC 2 cut(s) 506, 522
PstNI CAGNNNCTG 1 cut(s) 688
RsaI GTAC 3 cut(s) 62, 296, 462
RsaNI GTAC 3 cut(s) 61, 295, 461
SacI GAGCTC 1 cut(s) 249
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 2 cut(s) 435, 914
Sau3AI GATC 2 cut(s) 421, 965
Sau96I GGNCC 2 cut(s) 506, 522
ScaI AGTACT 1 cut(s) 62
SchI GAGTC 4 cut(s) 735, 858, 868, 919
ScrFI CCNGG 2 cut(s) 324, 731
SduI GDGCHC 3 cut(s) 249, 456, 760
SfaNI GCATC 3 cut(s) 4, 241, 523
SfcI CTRYAG 1 cut(s) 257
SfuI TTCGAA 1 cut(s) 109
SinI GGWCC 1 cut(s) 506
Sse9I AATT 1 cut(s) 263
SsiI CCGC 3 cut(s) 77, 434, 437
SspI AATATT 1 cut(s) 223
SspMI CTAG 3 cut(s) 408, 569, 979
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 2 cut(s) 322, 729
StyI CCWWGG 3 cut(s) 447, 502, 880
TaaI ACNGT 3 cut(s) 135, 149, 397
TaiI ACGT 2 cut(s) 290, 802
TaqI TCGA 7 cut(s) 5, 109, 243, 249, 358, 855, 948
TasI AATT 1 cut(s) 263
TatI WGTACW 1 cut(s) 60
TauI GCSGC 1 cut(s) 437
TfiI GAWTC 4 cut(s) 80, 95, 685, 860
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 2 cut(s) 489, 598
TseI GCWGC 1 cut(s) 913
TspDTI ATGAA 4 cut(s) 200, 293, 381, 444
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 2 cut(s) 489, 598
Van91I CCANNNNNTGG 1 cut(s) 597
VpaK11BI GGWCC 1 cut(s) 506
XapI RAATTY 1 cut(s) 263
XceI RCATGY 2 cut(s) 764, 824
XcmI CCANNNNNNNNNTGG 1 cut(s) 516
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 3 cut(s) 408, 569, 979
ZrmI AGTACT 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.