AT1G70480

Domain of unknown function DUF220

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001775
Physical Location & Seq
Forward (+)
26561716 .. 26563952
2237 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G70480.2

Sequence Viewer

Length: 963 bp
ATGGGCAATCCGGGATCAGACACAGAGAGAAACAAGGGAAATGTGGTCTCAAGTGTGTTTGATCCTGCACTCTCTCGATTCGTTGGATTTTCCCAGAAACTCCAAACTCGTCTTAAGTCCCAGCTGAAGAATTTGACAGATGATAAGCATGGGATTGGTTCTTCATGGAGGCAAGATAAAGGAAACTCATCGATCTCCATGCAAATTGATCTGGAGAAACAGTTAGATTGTTGGAGGGAAAACCCTTCGTGGACTGATCAGATCCCAGTCGTTAAGGTGGGTATTCCAAAAGGATCGCTTTGCAATCTTAAAGCCGAGGTGAATGTTGGTTTACCTCCTGATGCAGTCTATAACATTGTAATTGACCCTGACAATAGAAGAGTCTTCAAGAATATCAAGGAGGTTATGTCGCGGAAAGTATTGGTAGATGACGGGTTAAGGCAAGTGGTGGAAGTTGAACAAGCAGCTTTGTGGAGATTTCTCTGGTGGTCAGGAACAATCTCAGTTCATGTCTTGGTGGATCAGGATCGGGCAGATCACTCTATGAAATTCAAGCAAGTGAAAAGTGGATTCATGAAGAGATTCGAAGGAAGCTGGCAAGTAAAGCCTTTGTTTGTGGATGAACATATGTGCGATCGTTTGAAACCGAAAACGCTGGAGGAGTATAACAGGTGCACTGGAGGTAAAGGGAGGATCGGATCTAAGGTAACGCTGGACCAGCTAATACAGCCAGCGATTGTTCCACCTCCGCCCATTTCTTGGTATCTCAGAGGAATCACGGCTAAGACGACAGAGATGCTTATCCATGACTTGTTGGCTGAGACCGCCAGGATCCGTGCAGCTGGAGTAATGGAGGATGGTCACTCGCCCGATGAGCAACGGATTGGCACACCTGGAGATATAAAAGAAAGATGGTCTGCACATAGAAGAACATCAAGGAGACGACGCAATGATTTGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF220

Protein Analysis

320

Amino Acids

36.34

Weight (kDa)

9.4

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF220 PF02713 182 - 253 2e-32 Domain of unknown function DUF220
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 759
AccII CGCG 1 cut(s) 412
AciI CCGC 3 cut(s) 412, 749, 825
AcsI RAATTY 2 cut(s) 130, 548
AcuI CTGAAG 1 cut(s) 146
AfiI CCNNNNNNNGG 1 cut(s) 759
AflII CTTAAG 1 cut(s) 113
AgsI TTSAA 4 cut(s) 388, 458, 553, 643
AjnI CCWGG 2 cut(s) 827, 892
AluBI AGCT 5 cut(s) 124, 467, 594, 721, 842
AluI AGCT 5 cut(s) 124, 467, 594, 721, 842
Alw21I GWGCWC 1 cut(s) 677
Alw26I GTCTC 3 cut(s) 52, 815, 934
Alw44I GTGCAC 1 cut(s) 673
ApaLI GTGCAC 1 cut(s) 673
ApeKI GCWGC 2 cut(s) 464, 839
ApoI RAATTY 2 cut(s) 130, 548
Asp700I GAANNNNTTC 1 cut(s) 581
AspS9I GGNCC 1 cut(s) 715
AsuC2I CCSGG 1 cut(s) 12
AsuHPI GGTGA 1 cut(s) 331
AsuII TTCGAA 1 cut(s) 585
AvaII GGWCC 1 cut(s) 715
BaeGI GKGCMC 1 cut(s) 677
BamHI GGATCC 1 cut(s) 831
BbsI GAAGAC 1 cut(s) 376
Bbv12I GWGCWC 1 cut(s) 677
BbvI GCAGC 2 cut(s) 476, 851
BccI CCATC 2 cut(s) 851, 906
BceAI ACGGC 1 cut(s) 795
BcgI CGANNNNNNTGC 4 cut(s) 181, 215, 778, 812
BciT130I CCWGG 2 cut(s) 829, 894
BclI TGATCA 1 cut(s) 256
BcnI CCSGG 1 cut(s) 12
BcoDI GTCTC 3 cut(s) 52, 815, 934
BfrI CTTAAG 1 cut(s) 113
BisI GCNGC 2 cut(s) 465, 840
BlsI GCNGC 2 cut(s) 466, 841
Bme1390I CCNGG 3 cut(s) 12, 829, 894
Bme18I GGWCC 1 cut(s) 715
BmgT120I GGNCC 1 cut(s) 715
BmiI GGNNCC 1 cut(s) 833
BmrFI CCNGG 3 cut(s) 12, 829, 894
BmrI ACTGGG 1 cut(s) 260
BmsI GCATC 2 cut(s) 331, 786
BmuI ACTGGG 1 cut(s) 260
BpiI GAAGAC 1 cut(s) 376
BpmI CTGGAG 5 cut(s) 233, 677, 699, 864, 915
Bpu14I TTCGAA 1 cut(s) 585
BpuEI CTTGAG 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 12
Bsa29I ATCGAT 1 cut(s) 191
BsaBI GATNNNNATC 2 cut(s) 525, 800
BsaI GGTCTC 2 cut(s) 52, 815
BsaJI CCNNGG 1 cut(s) 315
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 392, 422
Bsc4I CCNNNNNNNGG 1 cut(s) 759
Bse1I ACTGG 2 cut(s) 266, 682
Bse3DI GCAATG 1 cut(s) 955
Bse8I GATNNNNATC 2 cut(s) 525, 800
BseBI CCWGG 2 cut(s) 829, 894
BseCI ATCGAT 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 2 cut(s) 625, 862
BseJI GATNNNNATC 2 cut(s) 525, 800
BseLI CCNNNNNNNGG 1 cut(s) 759
BseMI GCAATG 1 cut(s) 955
BseMII CTCAG 3 cut(s) 516, 781, 810
BseNI ACTGG 2 cut(s) 266, 682
BseRI GAGGAG 1 cut(s) 674
BseSI GKGCMC 1 cut(s) 677
BseXI GCAGC 2 cut(s) 476, 851
BseYI CCCAGC 1 cut(s) 120
BsgI GTGCAG 3 cut(s) 51, 858, 903
Bsh1236I CGCG 1 cut(s) 412
Bsh1285I CGRYCG 1 cut(s) 637
BshVI ATCGAT 1 cut(s) 191
BsiEI CGRYCG 1 cut(s) 637
BsiHKAI GWGCWC 1 cut(s) 677
BsiSI CCGG 1 cut(s) 11
BslFI GGGAC 1 cut(s) 103
BslI CCNNNNNNNGG 1 cut(s) 759
BsmAI GTCTC 3 cut(s) 52, 815, 934
BsmBI CGTCTC 1 cut(s) 934
BsmFI GGGAC 1 cut(s) 103
Bso31I GGTCTC 2 cut(s) 52, 815
Bsp119I TTCGAA 1 cut(s) 585
Bsp1286I GDGCHC 1 cut(s) 677
BspACI CCGC 3 cut(s) 412, 749, 825
BspCNI CTCAG 3 cut(s) 515, 780, 811
BspDI ATCGAT 1 cut(s) 191
BspFNI CGCG 1 cut(s) 412
BspHI TCATGA 1 cut(s) 573
BspLI GGNNCC 1 cut(s) 833
BspT104I TTCGAA 1 cut(s) 585
BspTI CTTAAG 1 cut(s) 113
BspTNI GGTCTC 2 cut(s) 52, 815
BsrDI GCAATG 1 cut(s) 955
BsrI ACTGG 2 cut(s) 266, 682
BssECI CCNNGG 1 cut(s) 315
Bst2UI CCWGG 2 cut(s) 829, 894
Bst4CI ACNGT 1 cut(s) 222
Bst6I CTCTTC 2 cut(s) 373, 572
BstAFI CTTAAG 1 cut(s) 113
BstBI TTCGAA 1 cut(s) 585
BstC8I GCNNGC 2 cut(s) 596, 732
BstDEI CTNAG 5 cut(s) 502, 702, 767, 783, 819
BstF5I GGATG 2 cut(s) 625, 862
BstFNI CGCG 1 cut(s) 412
BstMAI GTCTC 3 cut(s) 52, 815, 934
BstMCI CGRYCG 1 cut(s) 637
BstMWI GCNNNNNNNGC 5 cut(s) 604, 718, 727, 824, 874
BstNI CCWGG 2 cut(s) 829, 894
BstSCI CCNGG 3 cut(s) 10, 827, 892
BstSLI GKGCMC 1 cut(s) 677
BstUI CGCG 1 cut(s) 412
BstV1I GCAGC 2 cut(s) 476, 851
BstV2I GAAGAC 1 cut(s) 376
BstX2I RGATCY 3 cut(s) 261, 698, 831
BstYI RGATCY 3 cut(s) 261, 698, 831
Bsu15I ATCGAT 1 cut(s) 191
BsuTUI ATCGAT 1 cut(s) 191
BtsCI GGATG 2 cut(s) 625, 862
BtsIMutI CAGTG 1 cut(s) 675
Cac8I GCNNGC 2 cut(s) 596, 732
CciI TCATGA 1 cut(s) 573
Cfr13I GGNCC 1 cut(s) 715
ClaI ATCGAT 1 cut(s) 191
CseI GACGC 1 cut(s) 954
CviAII CATG 6 cut(s) 149, 165, 199, 509, 574, 806
DdeI CTNAG 5 cut(s) 502, 702, 767, 783, 819
Eam1104I CTCTTC 2 cut(s) 373, 572
EarI CTCTTC 2 cut(s) 373, 572
EciI GGCGGA 1 cut(s) 738
Eco31I GGTCTC 2 cut(s) 52, 815
Eco47I GGWCC 1 cut(s) 715
Eco57I CTGAAG 1 cut(s) 146
EcoRII CCWGG 2 cut(s) 827, 892
Esp3I CGTCTC 1 cut(s) 934
FaeI CATG 6 cut(s) 152, 168, 202, 512, 577, 809
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FaqI GGGAC 1 cut(s) 103
FatI CATG 6 cut(s) 148, 164, 198, 508, 573, 805
FauNDI CATATG 1 cut(s) 627
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 2 cut(s) 465, 840
FokI GGATG 2 cut(s) 632, 869
Fsp4HI GCNGC 2 cut(s) 465, 840
GluI GCNGC 2 cut(s) 465, 840
GsaI CCCAGC 1 cut(s) 124
GsuI CTGGAG 5 cut(s) 233, 677, 699, 864, 915
HapII CCGG 1 cut(s) 11
HgaI GACGC 1 cut(s) 954
Hin1II CATG 6 cut(s) 152, 168, 202, 512, 577, 809
HinfI GANTC 5 cut(s) 78, 381, 570, 582, 774
HpaII CCGG 1 cut(s) 11
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 3 cut(s) 252, 332, 675
Hpy188I TCNGA 4 cut(s) 19, 261, 698, 770
Hpy188III TCNNGA 7 cut(s) 75, 212, 338, 388, 492, 524, 574
Hpy8I GTNNAC 3 cut(s) 252, 332, 675
Hpy99I CGWCG 1 cut(s) 948
HpyAV CCTTC 2 cut(s) 255, 581
HpyCH4III ACNGT 1 cut(s) 222
HpyCH4V TGCA 7 cut(s) 68, 202, 303, 344, 675, 839, 920
HpyF10VI GCNNNNNNNGC 5 cut(s) 604, 718, 727, 824, 874
HpyF3I CTNAG 5 cut(s) 502, 702, 767, 783, 819
Hsp92II CATG 6 cut(s) 152, 168, 202, 512, 577, 809
Ksp22I TGATCA 1 cut(s) 256
Lsp1109I GCAGC 2 cut(s) 476, 851
LweI GCATC 2 cut(s) 331, 786
MaeIII GTNAC 2 cut(s) 706, 860
MboII GAAGA 6 cut(s) 139, 153, 376, 390, 589, 939
MflI RGATCY 3 cut(s) 261, 698, 831
MhlI GDGCHC 1 cut(s) 677
MluCI AATT 4 cut(s) 130, 204, 360, 548
MlyI GAGTC 1 cut(s) 390
MmeI TCCRAC 2 cut(s) 64, 212
MroXI GAANNNNTTC 1 cut(s) 581
MseI TTAA 5 cut(s) 114, 273, 309, 437, 961
MspA1I CMGCKG 2 cut(s) 124, 842
MspCI CTTAAG 1 cut(s) 113
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 3 cut(s) 12, 829, 894
MvaI CCWGG 2 cut(s) 829, 894
MvnI CGCG 1 cut(s) 412
MwoI GCNNNNNNNGC 5 cut(s) 604, 718, 727, 824, 874
NciI CCSGG 1 cut(s) 12
NdeI CATATG 1 cut(s) 627
NlaIII CATG 6 cut(s) 152, 168, 202, 512, 577, 809
NlaIV GGNNCC 1 cut(s) 833
NmeAIII GCCGAG 1 cut(s) 340
NmuCI GTSAC 1 cut(s) 860
NspV TTCGAA 1 cut(s) 585
PagI TCATGA 1 cut(s) 573
PcsI WCGNNNNNNNCGW 1 cut(s) 785
PdmI GAANNNNTTC 1 cut(s) 581
PfeI GAWTC 4 cut(s) 78, 570, 582, 774
PflMI CCANNNNNTGG 1 cut(s) 759
PfoI TCCNGGA 1 cut(s) 10
PkrI GCNGC 2 cut(s) 466, 841
Ple19I CGATCG 1 cut(s) 637
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
Psp6I CCWGG 2 cut(s) 827, 892
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 2 cut(s) 827, 892
PspN4I GGNNCC 1 cut(s) 833
PspPI GGNCC 1 cut(s) 715
PsuI RGATCY 3 cut(s) 261, 698, 831
PvuI CGATCG 1 cut(s) 637
PvuII CAGCTG 2 cut(s) 124, 842
SaqAI TTAA 5 cut(s) 114, 273, 309, 437, 961
SatI GCNGC 2 cut(s) 465, 840
Sau96I GGNCC 1 cut(s) 715
SchI GAGTC 1 cut(s) 390
ScrFI CCNGG 3 cut(s) 12, 829, 894
SduI GDGCHC 1 cut(s) 677
SfaNI GCATC 2 cut(s) 331, 786
SfuI TTCGAA 1 cut(s) 585
SinI GGWCC 1 cut(s) 715
SmlI CTYRAG 2 cut(s) 49, 113
SmoI CTYRAG 2 cut(s) 49, 113
Sse9I AATT 4 cut(s) 130, 204, 360, 548
SsiI CCGC 3 cut(s) 412, 749, 825
StyD4I CCNGG 3 cut(s) 10, 827, 892
TaaI ACNGT 1 cut(s) 222
TaqI TCGA 3 cut(s) 76, 191, 585
TasI AATT 4 cut(s) 130, 204, 360, 548
TfiI GAWTC 4 cut(s) 78, 570, 582, 774
Tru1I TTAA 5 cut(s) 114, 273, 309, 437, 961
Tru9I TTAA 5 cut(s) 114, 273, 309, 437, 961
TscAI CASTG 1 cut(s) 682
TseFI GTSAC 1 cut(s) 860
TseI GCWGC 2 cut(s) 464, 839
Tsp45I GTSAC 1 cut(s) 860
TspDTI ATGAA 6 cut(s) 153, 497, 560, 562, 590, 636
TspGWI ACGGA 2 cut(s) 824, 895
TspRI CASTG 1 cut(s) 682
Van91I CCANNNNNTGG 1 cut(s) 759
Vha464I CTTAAG 1 cut(s) 113
VneI GTGCAC 1 cut(s) 673
VpaK11BI GGWCC 1 cut(s) 715
XapI RAATTY 2 cut(s) 130, 548
XmnI GAANNNNTTC 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.