FvH4_4g34241

Domain of unknown function DUF220

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001775
Physical Location & Seq
Reverse (-)
32137302 .. 32140114
2813 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34241.t1

Sequence Viewer

Length: 999 bp
ATGGGTGAGACTGAGGTGCCCACGACAAGCATAGACATCAGCAAGAATGAGAAAAATGGGCGCCTCAAGGCATTGGATGTAGTGCTCTTGAAAGCTTTTGTCCAGGTTCCTCACAAGCTACAGAATTTTCTTAAGTCACGGCTTAGAAGACTGTCAAATGCTGATGGAGTTAAAACAGTGAGCTCTGTCCCTAAAGTGACTTCATCTTTGGAGGTTGACCTGGAAAAGCAGTTGCAAGCTTGGAGAGAAAATCCTTCATGGGAAGATCAAACACCACAAATAAAGGTCAGTGTGCCAAAAGGTTCTTTGTGCAACCTCAGTGTAAACGTCAATGTTGGGTTGCCACCGGATGCAGTATATAATATTGTTACTGACCCTGACAATAAGAGGGTTTTCAAGAATATCAAGGAGGTCATATCCAGAAGAGTTTTGGTTGATGAAGGTCTTAGGCAGGTGGTTGAACTAGAGCAAGCAGCTTTATGGAGGTTTCTTTGGTGGTCAGGGACCATTGCAGTTCATGTTTTAGTAGATCAAAACAGAGAAGACCACTCAATGAAGTTCAAGCAAGTTAAAACAGGGTTTATGGAGAGATTCGAAGGTTGCTGGACAGTGGAGCCTCTTTTTGTTGATGAAAAAAGCTGCCTTCCCTTTAAACCCACAACATGGGCAGACTATAATTCACACACGGGAGGCAAGGGAAGGATTGGATCAAAAGTGAGCTTGGACCAACTGATCCAACCGTCTATTGTTCCTCCTCCGCCCATTTCTTGGTATCTAAGGGGTATCACTGCCAAGACCACAGAGATGTTGATAAATGATCTTCTTGCTGAAGCCGCCAGGATACGGGGAGAATTGAACCCTGAAAAGTCTGGTGAAGAGGTTGAAATGTCTGAGGAAGTTCTTGGTCAAGTTGATAAAACATTTAACATCAAAGAAAGATGGGCCATGCATAGGAGAAATGCAAAGCAATGTCATCGGAGGCTGCCGATTGCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF220

Protein Analysis

333

Amino Acids

37.71

Weight (kDa)

9.03

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF220 PF02713 167 - 238 1.5e-28 Domain of unknown function DUF220
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 442
Acc36I ACCTGC 1 cut(s) 442
AccB1I GGYRCC 2 cut(s) 16, 60
AccB7I CCANNNNNTGG 2 cut(s) 663, 768
AciI CCGC 2 cut(s) 758, 834
AclWI GGATC 2 cut(s) 715, 727
AcsI RAATTY 1 cut(s) 124
AcuI CTGAAG 1 cut(s) 849
AcyI GRCGYC 1 cut(s) 61
AfiI CCNNNNNNNGG 4 cut(s) 663, 768, 843, 951
AflII CTTAAG 1 cut(s) 131
AgsI TTSAA 6 cut(s) 91, 397, 461, 562, 856, 884
AjnI CCWGG 3 cut(s) 102, 219, 836
AluBI AGCT 7 cut(s) 95, 118, 183, 239, 476, 639, 720
AluI AGCT 7 cut(s) 95, 118, 183, 239, 476, 639, 720
Alw21I GWGCWC 2 cut(s) 87, 185
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 2 cut(s) 715, 727
AoxI GGCC 1 cut(s) 942
ApeKI GCWGC 3 cut(s) 473, 639, 982
ApoI RAATTY 1 cut(s) 124
ArsI GACNNNNNNTTYG 2 cut(s) 190, 222
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 3 cut(s) 504, 724, 942
AsuHPI GGTGA 2 cut(s) 17, 884
AsuII TTCGAA 1 cut(s) 594
AvaII GGWCC 2 cut(s) 504, 724
BaeGI GKGCMC 1 cut(s) 21
BanI GGYRCC 2 cut(s) 16, 60
BanII GRGCYC 1 cut(s) 185
BbsI GAAGAC 2 cut(s) 154, 549
Bbv12I GWGCWC 2 cut(s) 87, 185
BbvI GCAGC 3 cut(s) 485, 626, 969
BccI CCATC 2 cut(s) 158, 933
BceAI ACGGC 1 cut(s) 155
BcgI CGANNNNNNTGC 2 cut(s) 956, 990
BciT130I CCWGG 3 cut(s) 104, 221, 838
BciVI GTATCC 1 cut(s) 834
BcoDI GTCTC 1 cut(s) 2
BfaI CTAG 1 cut(s) 464
BfmI CTRYAG 1 cut(s) 119
BfoI RGCGCY 1 cut(s) 64
BfrI CTTAAG 1 cut(s) 131
BfuAI ACCTGC 1 cut(s) 442
BfuI GTATCC 1 cut(s) 834
BisI GCNGC 4 cut(s) 474, 640, 834, 983
BlsI GCNGC 4 cut(s) 475, 641, 835, 984
Bme1390I CCNGG 3 cut(s) 104, 221, 838
Bme18I GGWCC 2 cut(s) 504, 724
BmgT120I GGNCC 3 cut(s) 504, 724, 942
BmiI GGNNCC 5 cut(s) 18, 62, 108, 505, 615
BmrFI CCNGG 3 cut(s) 104, 221, 838
BmsI GCATC 1 cut(s) 340
BpiI GAAGAC 2 cut(s) 154, 549
Bpu14I TTCGAA 1 cut(s) 594
BpuEI CTTGAG 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 61
BsaWI WCCGGW 1 cut(s) 346
Bsc4I CCNNNNNNNGG 4 cut(s) 663, 768, 843, 951
Bse3DI GCAATG 2 cut(s) 507, 974
BseBI CCWGG 3 cut(s) 104, 221, 838
BseGI GGATG 2 cut(s) 82, 355
BseLI CCNNNNNNNGG 4 cut(s) 663, 768, 843, 951
BseMI GCAATG 2 cut(s) 507, 974
BseMII CTCAG 2 cut(s) 331, 882
BseRI GAGGAG 1 cut(s) 744
BseSI GKGCMC 1 cut(s) 21
BseXI GCAGC 3 cut(s) 485, 626, 969
BshFI GGCC 1 cut(s) 944
BshNI GGYRCC 2 cut(s) 16, 60
BsiHKAI GWGCWC 2 cut(s) 87, 185
BsiSI CCGG 1 cut(s) 347
BslFI GGGAC 2 cut(s) 173, 517
BslI CCNNNNNNNGG 4 cut(s) 663, 768, 843, 951
BsmAI GTCTC 1 cut(s) 2
BsmFI GGGAC 2 cut(s) 173, 517
BsnI GGCC 1 cut(s) 944
Bsp119I TTCGAA 1 cut(s) 594
Bsp1286I GDGCHC 3 cut(s) 21, 87, 185
Bsp143I GATC 5 cut(s) 265, 529, 707, 732, 817
BspACI CCGC 2 cut(s) 758, 834
BspANI GGCC 1 cut(s) 944
BspCNI CTCAG 3 cut(s) 4, 330, 883
BspLI GGNNCC 5 cut(s) 18, 62, 108, 505, 615
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 2 cut(s) 715, 727
BspT104I TTCGAA 1 cut(s) 594
BspT107I GGYRCC 2 cut(s) 16, 60
BspTI CTTAAG 1 cut(s) 131
BsrDI GCAATG 2 cut(s) 507, 974
BssMI GATC 5 cut(s) 265, 529, 707, 732, 817
BssNI GRCGYC 1 cut(s) 61
Bst2UI CCWGG 3 cut(s) 104, 221, 838
Bst4CI ACNGT 4 cut(s) 153, 178, 610, 741
Bst6I CTCTTC 2 cut(s) 418, 870
BstACI GRCGYC 1 cut(s) 61
BstAFI CTTAAG 1 cut(s) 131
BstBI TTCGAA 1 cut(s) 594
BstC8I GCNNGC 2 cut(s) 237, 471
BstDEI CTNAG 6 cut(s) 12, 143, 317, 446, 776, 891
BstF5I GGATG 2 cut(s) 82, 355
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 5 cut(s) 268, 532, 710, 735, 820
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 5 cut(s) 265, 529, 707, 732, 817
BstMWI GCNNNNNNNGC 1 cut(s) 833
BstNI CCWGG 3 cut(s) 104, 221, 838
BstSCI CCNGG 3 cut(s) 102, 219, 836
BstSFI CTRYAG 1 cut(s) 119
BstSLI GKGCMC 1 cut(s) 21
BstV1I GCAGC 3 cut(s) 485, 626, 969
BstV2I GAAGAC 2 cut(s) 154, 549
BsuI GTATCC 1 cut(s) 834
BsuRI GGCC 1 cut(s) 944
BtsCI GGATG 2 cut(s) 82, 355
BtsI GCAGTG 1 cut(s) 786
BtsIMutI CAGTG 5 cut(s) 183, 295, 325, 615, 786
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 2 cut(s) 237, 471
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 3 cut(s) 504, 724, 942
CviAII CATG 4 cut(s) 258, 518, 663, 946
DdeI CTNAG 6 cut(s) 12, 143, 317, 446, 776, 891
DinI GGCGCC 1 cut(s) 62
DpnI GATC 5 cut(s) 267, 531, 709, 734, 819
DpnII GATC 5 cut(s) 265, 529, 707, 732, 817
DraI TTTAAA 1 cut(s) 652
Eam1104I CTCTTC 2 cut(s) 418, 870
EarI CTCTTC 2 cut(s) 418, 870
EciI GGCGGA 1 cut(s) 747
Ecl136II GAGCTC 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 185
Eco47I GGWCC 2 cut(s) 504, 724
Eco53kI GAGCTC 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 849
EcoICRI GAGCTC 1 cut(s) 183
EcoRII CCWGG 3 cut(s) 102, 219, 836
EcoT22I ATGCAT 1 cut(s) 951
EcoT38I GRGCYC 1 cut(s) 185
EgeI GGCGCC 1 cut(s) 62
EheI GGCGCC 1 cut(s) 62
FaeI CATG 4 cut(s) 261, 521, 666, 949
FaqI GGGAC 2 cut(s) 173, 517
FatI CATG 4 cut(s) 257, 517, 662, 945
Fnu4HI GCNGC 4 cut(s) 474, 640, 834, 983
FokI GGATG 2 cut(s) 89, 362
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 4 cut(s) 474, 640, 834, 983
FspBI CTAG 1 cut(s) 464
GlaI GCGC 1 cut(s) 62
GluI GCNGC 4 cut(s) 474, 640, 834, 983
HaeII RGCGCY 1 cut(s) 64
HaeIII GGCC 1 cut(s) 944
HapII CCGG 1 cut(s) 347
HhaI GCGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 61
Hin1II CATG 4 cut(s) 261, 521, 666, 949
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 2 cut(s) 93, 237
HinfI GANTC 1 cut(s) 591
HpaII CCGG 1 cut(s) 347
HphI GGTGA 2 cut(s) 17, 884
Hpy166II GTNNAC 2 cut(s) 217, 325
Hpy188I TCNGA 2 cut(s) 892, 978
Hpy188III TCNNGA 3 cut(s) 88, 397, 420
Hpy8I GTNNAC 2 cut(s) 217, 325
HpyAV CCTTC 5 cut(s) 264, 434, 590, 653, 693
HpyCH4III ACNGT 4 cut(s) 153, 178, 610, 741
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 6 cut(s) 235, 312, 353, 512, 949, 962
HpyF10VI GCNNNNNNNGC 1 cut(s) 833
HpyF3I CTNAG 6 cut(s) 12, 143, 317, 446, 776, 891
HpySE526I ACGT 1 cut(s) 327
Hsp92I GRCGYC 1 cut(s) 61
Hsp92II CATG 4 cut(s) 261, 521, 666, 949
HspAI GCGC 1 cut(s) 61
KasI GGCGCC 1 cut(s) 60
Kzo9I GATC 5 cut(s) 265, 529, 707, 732, 817
LmnI GCTCC 1 cut(s) 613
Lsp1109I GCAGC 3 cut(s) 485, 626, 969
LweI GCATC 1 cut(s) 340
MaeI CTAG 1 cut(s) 464
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 3 cut(s) 135, 196, 367
MalI GATC 5 cut(s) 267, 531, 709, 734, 819
MboI GATC 5 cut(s) 265, 529, 707, 732, 817
MboII GAAGA 6 cut(s) 159, 275, 435, 554, 812, 887
MhlI GDGCHC 3 cut(s) 21, 87, 185
MluCI AATT 3 cut(s) 124, 676, 851
Mly113I GGCGCC 1 cut(s) 61
MmeI TCCRAC 1 cut(s) 760
Mph1103I ATGCAT 1 cut(s) 951
MseI TTAA 5 cut(s) 132, 171, 570, 651, 924
MslI CAYNNNNRTG 1 cut(s) 803
MspCI CTTAAG 1 cut(s) 131
MspI CCGG 1 cut(s) 347
MspR9I CCNGG 3 cut(s) 104, 221, 838
MvaI CCWGG 3 cut(s) 104, 221, 838
MwoI GCNNNNNNNGC 1 cut(s) 833
NarI GGCGCC 1 cut(s) 61
NdeII GATC 5 cut(s) 265, 529, 707, 732, 817
NlaIII CATG 4 cut(s) 261, 521, 666, 949
NlaIV GGNNCC 5 cut(s) 18, 62, 108, 505, 615
NmuCI GTSAC 2 cut(s) 135, 196
NsiI ATGCAT 1 cut(s) 951
NspV TTCGAA 1 cut(s) 594
PaqCI CACCTGC 1 cut(s) 442
PfeI GAWTC 1 cut(s) 591
PflMI CCANNNNNTGG 2 cut(s) 663, 768
PkrI GCNGC 4 cut(s) 475, 641, 835, 984
PluTI GGCGCC 1 cut(s) 64
Psp124BI GAGCTC 1 cut(s) 185
Psp6I CCWGG 3 cut(s) 102, 219, 836
PspGI CCWGG 3 cut(s) 102, 219, 836
PspN4I GGNNCC 5 cut(s) 18, 62, 108, 505, 615
PspPI GGNCC 3 cut(s) 504, 724, 942
RseI CAYNNNNRTG 1 cut(s) 803
SacI GAGCTC 1 cut(s) 185
SaqAI TTAA 5 cut(s) 132, 171, 570, 651, 924
SatI GCNGC 4 cut(s) 474, 640, 834, 983
Sau3AI GATC 5 cut(s) 265, 529, 707, 732, 817
Sau96I GGNCC 3 cut(s) 504, 724, 942
ScrFI CCNGG 3 cut(s) 104, 221, 838
SduI GDGCHC 3 cut(s) 21, 87, 185
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 119
SfoI GGCGCC 1 cut(s) 62
SfuI TTCGAA 1 cut(s) 594
SinI GGWCC 2 cut(s) 504, 724
SmiMI CAYNNNNRTG 1 cut(s) 803
SmlI CTYRAG 2 cut(s) 65, 131
SmoI CTYRAG 2 cut(s) 65, 131
Sse9I AATT 3 cut(s) 124, 676, 851
SsiI CCGC 2 cut(s) 758, 834
SspDI GGCGCC 1 cut(s) 60
SspI AATATT 1 cut(s) 364
SspMI CTAG 1 cut(s) 464
SstI GAGCTC 1 cut(s) 185
StyD4I CCNGG 3 cut(s) 102, 219, 836
TaaI ACNGT 4 cut(s) 153, 178, 610, 741
TaiI ACGT 1 cut(s) 330
TaqI TCGA 1 cut(s) 594
TasI AATT 3 cut(s) 124, 676, 851
TauI GCSGC 1 cut(s) 836
TfiI GAWTC 1 cut(s) 591
Tru1I TTAA 5 cut(s) 132, 171, 570, 651, 924
Tru9I TTAA 5 cut(s) 132, 171, 570, 651, 924
TscAI CASTG 5 cut(s) 183, 295, 325, 615, 793
TseFI GTSAC 2 cut(s) 135, 196
TseI GCWGC 3 cut(s) 473, 639, 982
Tsp45I GTSAC 2 cut(s) 135, 196
TspDTI ATGAA 6 cut(s) 192, 246, 453, 506, 569, 645
TspRI CASTG 5 cut(s) 183, 295, 325, 615, 793
Van91I CCANNNNNTGG 2 cut(s) 663, 768
Vha464I CTTAAG 1 cut(s) 131
VpaK11BI GGWCC 2 cut(s) 504, 724
XapI RAATTY 1 cut(s) 124
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XspI CTAG 1 cut(s) 464
Zsp2I ATGCAT 1 cut(s) 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.