Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0001775

Physical Location & Seq

Genomic Coordinates Reverse (-)

61847727 .. 61850379

Gene Structure 2653 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G034920.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1884 bp

ATGGCGGACGGTGAAGATTCCGGTGCTGTAGCCGCTGAGCTCGTCAGGACTAGCACGCCTCTCGACTTCAAGACTCTTCTTTCTTCTCCGGCGAGGGACTTTCTTGTTCGCAACAATGGTGATCAGGTTAAGGTTGAAACCTTGAAGGGGAAGAAGCTGGGGTTGTATTTTTCTGCATCATGGTGCGGTCCATGTCAACGTTTCACCCCAGATTTGGTGGAGACCTACAATGTGCTCGCTCCAAAAGGCGATTTCGAGGTGATCTTTATTTCAGCTGATGAGGATGAGGAGTCGTTCAATGGGTACTTCTCCAAGATGCCGTGGCTTGCAATTCCGTTTTCGGATTCTGAGGCACGTGATAGTCTGGATGAGCAGTTCAAGGTGAGAGGGATACCACATCTTGTGTTCCTTTCTGAAGAAGGGAGAGTTGAGAATGCCAGTGGAGTTGAGATTGTTCGGGAGTATGGAGCTGATGGCTACCCTTTTACCATAGAAAGGACAAAAGAATTACAAGAACAAGAAGAGGCAGCTAAAAGGGAACAGTCTCTGAAAACAGTCCTGGTCTCCCGTTCACGCGACTTTGTAATTGCATCTGATGGGAAGAAGGTGCCTGTCTCTGAACTTGAAGGGAAGATGGTGGGTCTCTACTTCTCATTGTCTACATTCAGTCCATGCATAGAATTCACTCCAAAACTTGTGGAGGTTTATGAGAAGCTAAAAGCAAAGGGAGAGAGCTTTGAGATTGTGTTTATATCACTTGATGATGATGAAGAAGCATTTGCACAAGATTGTAAGAACATGCCTTGGTTTGCATTGCCCCAGAAGGACACCAAAACCATTGAGAAGTTGGCTCGGTATTTTGAGCTCTCAGCCTTACCCACTTTGGTTATTATTGGGGCAGATGGTAAAACTGTCCATAACAATGTTGTTGAAGCTATTGACGAGCATGGGCTTCTGGCTTACCCCTTCACACCTGAGAAATTTGCAGAGCTCGCTGAGATAGAGAAAGCGAAAGAGAAAGCACAAACTCTGGAGTCAATTTTGATTTCTGGGGATCAAAACTTTGTCATTGGAAAAGACGGAATCAAGATTCCGGTGTCAGATCTTGTGGGGAAGAACATCCTCCTTTATTTCTCAGCACACTGGTGCCCTCCATGCCGTGCCTTTCTACCAAAACTTATGGAAGCCTATGACAAGATCAAGGCAAAGGATGATGCATTTGAAGTTATTTTCATCTCTAGTGACAGGGACCAAGCCTCGTTTGATGACTTTTTCTCTGGAATGCCATGGCTTGCTCTTCCTTTTGGTGACTCAAGAAAGGCATCCTTGAGTCGCCGATTCAAGGTCCAAGGCATCCCCATGCTTGTAGCCATTGGTCCTACTGGTCAAACAGTCACAAAAGAAGCAATACGTCTCGTTATGCTTCATGGAGCTAATGCTTATCCTTTCACTGAGGAGCGGCTGGAAGAGATAGAAGCAGAATTTAAGGAGATGGCAAAGAGGTGGCCTGAGAAGTTGAAGAATGCACTCCATGAGGAGCATGAGCTTGTGCTCTGTCGCCGAAGAAGTTTCATATGTGATGGGTGCAACGAGAAAGGAGAGGTGTGGTCATTCTATTGCGAGGAGTGTGACTTTGATCTCCATCCAAAGTGTGCTCTGGAGGAAGAAAATGGAACCAAAACTGATGCCAACCCGGAAGAAGATCCTCATCCAAAGTGTGCTTTGGAGGAGGAGAAGGGAACCAAAACTGATGCCAAGCCGGAAGAAGATCCCCATCCAAAGTGTGCTTTGGAGGAGGAAAAGGGAACCAAAACTGATGCCAAGCCGGAAGAAGAGCCCCAAGAAGGATGGGTATGCGATGGTGAAGTTTGTAAAAAAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0003824 GO:0004791 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006935 GO:0008150 GO:0008152 GO:0009605 GO:0009636 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010035 GO:0010038 GO:0010183 GO:0010769 GO:0015036 GO:0016043 GO:0016049 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0022414 GO:0022603 GO:0022604 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0040008 GO:0040011 GO:0042221 GO:0042330 GO:0043900 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0045595 GO:0046686 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050918 GO:0051128 GO:0051239 GO:0051510 GO:0051704 GO:0051716 GO:0055114 GO:0060284 GO:0060560 GO:0065007 GO:0070887 GO:0071840 GO:0080092 GO:0097237 GO:0098754 GO:0098869 GO:1990748 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C1_2 Thioredoxin_8

Protein Analysis

627

Amino Acids

70.49

Weight (kDa)

4.79

Isoelectric Point (pI)

44.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AhpC-TSA	PF00578	30 - 143	2.6e-07	AhpC/TSA family
Redoxin	PF08534	33 - 144	2.6e-08	Redoxin
Thioredoxin_2	PF13098	49 - 144	2.8e-06	Thioredoxin-like domain
Thioredoxin_8	PF13905	50 - 143	1.9e-23	Thioredoxin-like
Thioredoxin_8	PF13905	210 - 303	2.5e-22	Thioredoxin-like
Redoxin	PF08534	358 - 450	7.8e-07	Redoxin
AhpC-TSA	PF00578	359 - 450	1.7e-08	AhpC/TSA family
Thioredoxin_8	PF13905	371 - 463	3e-27	Thioredoxin-like
Thioredoxin_2	PF13098	371 - 461	7.3e-07	Thioredoxin-like domain
C1_2	PF03107	510 - 552	4.5e-08	C1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70480 AT1G70480
fragaria_vesca	FvH4_4g34241 FvH4_4g34250 FvH4_4g34260 FvH4_4g34260 FvH4_4g34260 FvH4_4g34260
malus_domestica	MD13G1093300.v1.1 MD13G1093500.v1.1 MD16G1094500.v1.1 MD16G1094600.v1.1 MD16G1094700.v1.1
prunus_persica	Prupe.1G251900_v2.0.a1 Prupe.1G252000_v2.0.a1
pyrus_communis	pycom13g08130 pycom13g08140 pycom16g08030 pycom16g08040 pycom16g08050
rosa_chinensis	RchiOBHm_Chr4g0443711 RchiOBHm_Chr6g0250421
rosa_laevigata	RLG00000005898 RLG00000005899 RLG00000005900
rosa_multiflora	Rmu_co8463221.1_g000001 Rmu_sc0001426.1_g000010 Rmu_sc0001426.1_g000011 Rmu_sc0001426.1_g000013 Rmu_sc0008739.1_g000001
rosa_roxburghii	Rroxscaffold_5G00384250 Rroxscaffold_5G00384260 Rroxscaffold_5G00384270
rosa_rugosa	Rorug05G0496400 Rorug05G0496500
rosa_samantha	Rh4AG406400 Rh4AG406500 Rh4AG406600 Rh4BG417400 Rh4BG417500 Rh4BG417600 Rh4BG417700 Rh4CG431900 Rh4DG413000
rosa_wichuraiana	Rw4G034900 Rw4G034910 Rw4G034920

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	607, 1146
AccBSI	CCGCTC	1 cut(s)	1459
AccI	GTMKAC	1 cut(s)	659
AccII	CGCG	1 cut(s)	576
AciI	CCGC	4 cut(s)	5, 33, 186, 1459
AclI	AACGTT	1 cut(s)	199
AclWI	GGATC	3 cut(s)	1062, 1697, 1763
AcsI	RAATTY	3 cut(s)	680, 980, 1481
AcuI	CTGAAG	1 cut(s)	435
AcvI	CACGTG	1 cut(s)	356
AfaI	GTAC	1 cut(s)	305
AfiI	CCNNNNNNNGG	5 cut(s)	147, 214, 495, 1342, 1844
AjnI	CCWGG	1 cut(s)	558
AleI	CACNNNNGTG	1 cut(s)	1144
AloI	GAACNNNNNNTCC	2 cut(s)	359, 391
Alw21I	GWGCWC	6 cut(s)	42, 237, 867, 993, 1554, 1657
Alw26I	GTCTC	6 cut(s)	215, 549, 568, 619, 647, 1418
AlwI	GGATC	3 cut(s)	1062, 1697, 1763
AlwNI	CAGNNNCTG	1 cut(s)	547
AoxI	GGCC	1 cut(s)	1505
ApeKI	GCWGC	1 cut(s)	527
ApoI	RAATTY	3 cut(s)	680, 980, 1481
AspS9I	GGNCC	4 cut(s)	188, 1249, 1345, 1376
AsuC2I	CCSGG	1 cut(s)	1694
AsuHPI	GGTGA	7 cut(s)	23, 131, 196, 271, 394, 1319, 1874
AvaII	GGWCC	4 cut(s)	188, 1249, 1345, 1376
BaeGI	GKGCMC	1 cut(s)	1151
BanI	GGYRCC	2 cut(s)	607, 1146
BanII	GRGCYC	4 cut(s)	42, 867, 993, 1839
BbrPI	CACGTG	1 cut(s)	356
Bbv12I	GWGCWC	6 cut(s)	42, 237, 867, 993, 1554, 1657
BbvI	GCAGC	1 cut(s)	539
BceAI	ACGGC	2 cut(s)	304, 1143
BcgI	CGANNNNNNTGC	2 cut(s)	43, 77
BciT130I	CCWGG	1 cut(s)	560
BciVI	GTATCC	1 cut(s)	384
BclI	TGATCA	1 cut(s)	121
BcnI	CCSGG	1 cut(s)	1694
BcoDI	GTCTC	6 cut(s)	215, 549, 568, 619, 647, 1418
BfaI	CTAG	2 cut(s)	51, 1239
BfmI	CTRYAG	1 cut(s)	27
BfuI	GTATCC	1 cut(s)	384
BglII	AGATCT	1 cut(s)	1102
BisI	GCNGC	3 cut(s)	33, 528, 1460
BlpI	GCTNAGC	1 cut(s)	36
BlsI	GCNGC	3 cut(s)	34, 529, 1461
Bme1390I	CCNGG	2 cut(s)	560, 1694
Bme18I	GGWCC	4 cut(s)	188, 1249, 1345, 1376
BmgT120I	GGNCC	4 cut(s)	188, 1249, 1345, 1376
BmiI	GGNNCC	6 cut(s)	609, 1148, 1250, 1675, 1741, 1807
BmrFI	CCNGG	2 cut(s)	560, 1694
BmsI	GCATC	9 cut(s)	185, 306, 599, 1204, 1331, 1362, 1675, 1741, 1807
BpmI	CTGGAG	2 cut(s)	1052, 1679
Bpu1102I	GCTNAGC	1 cut(s)	36
BpuEI	CTTGAG	2 cut(s)	1297, 1348
BpuMI	CCSGG	1 cut(s)	1694
BsaAI	YACGTR	1 cut(s)	356
BsaBI	GATNNNNATC	3 cut(s)	1641, 1707, 1773
BsaI	GGTCTC	3 cut(s)	215, 568, 647
BsaJI	CCNNGG	4 cut(s)	320, 803, 1286, 1348
BsaWI	WCCGGW	2 cut(s)	20, 1093
Bsc4I	CCNNNNNNNGG	5 cut(s)	147, 214, 495, 1342, 1844
Bse1I	ACTGG	3 cut(s)	438, 1148, 1387
Bse3DI	GCAATG	1 cut(s)	812
Bse8I	GATNNNNATC	3 cut(s)	1641, 1707, 1773
BseBI	CCWGG	1 cut(s)	560
BseDI	CCNNGG	4 cut(s)	320, 803, 1286, 1348
BseJI	GATNNNNATC	3 cut(s)	1641, 1707, 1773
BseLI	CCNNNNNNNGG	5 cut(s)	147, 214, 495, 1342, 1844
BseMI	GCAATG	1 cut(s)	812
BseMII	CTCAG	8 cut(s)	27, 339, 882, 966, 987, 1149, 1443, 1500
BseNI	ACTGG	3 cut(s)	438, 1148, 1387
BseRI	GAGGAG	7 cut(s)	302, 1469, 1550, 1637, 1742, 1745, 1808
BseSI	GKGCMC	1 cut(s)	1151
BseXI	GCAGC	1 cut(s)	539
BseYI	CCCAGC	1 cut(s)	157
Bsh1236I	CGCG	1 cut(s)	576
BshFI	GGCC	1 cut(s)	1507
BshNI	GGYRCC	2 cut(s)	607, 1146
BsiHKAI	GWGCWC	6 cut(s)	42, 237, 867, 993, 1554, 1657
BsiSI	CCGG	6 cut(s)	21, 89, 1094, 1694, 1760, 1826
BslFI	GGGAC	2 cut(s)	110, 1262
BslI	CCNNNNNNNGG	5 cut(s)	147, 214, 495, 1342, 1844
BsmAI	GTCTC	6 cut(s)	215, 549, 568, 619, 647, 1418
BsmBI	CGTCTC	1 cut(s)	1418
BsmFI	GGGAC	2 cut(s)	110, 1262
BsmI	GAATGC	3 cut(s)	439, 1287, 1528
BsnI	GGCC	1 cut(s)	1507
Bso31I	GGTCTC	3 cut(s)	215, 568, 647
Bsp1286I	GDGCHC	8 cut(s)	42, 237, 867, 993, 1151, 1554, 1657, 1839
Bsp143I	GATC	8 cut(s)	121, 261, 1054, 1102, 1197, 1636, 1702, 1768
Bsp1720I	GCTNAGC	1 cut(s)	36
Bsp19I	CCATGG	1 cut(s)	1286
BspACI	CCGC	4 cut(s)	5, 33, 186, 1459
BspANI	GGCC	1 cut(s)	1507
BspCNI	CTCAG	8 cut(s)	28, 340, 881, 967, 988, 1148, 1444, 1501
BspFNI	CGCG	1 cut(s)	576
BspLI	GGNNCC	6 cut(s)	609, 1148, 1250, 1675, 1741, 1807
BspPI	GGATC	3 cut(s)	1062, 1697, 1763
BspQI	GCTCTTC	2 cut(s)	1302, 1827
BspT107I	GGYRCC	2 cut(s)	607, 1146
BspTNI	GGTCTC	3 cut(s)	215, 568, 647
BsrBI	CCGCTC	1 cut(s)	1459
BsrDI	GCAATG	1 cut(s)	812
BsrI	ACTGG	3 cut(s)	438, 1148, 1387
BssECI	CCNNGG	4 cut(s)	320, 803, 1286, 1348
BssMI	GATC	8 cut(s)	121, 261, 1054, 1102, 1197, 1636, 1702, 1768
BssT1I	CCWWGG	3 cut(s)	803, 1286, 1348
Bst2UI	CCWGG	1 cut(s)	560
Bst4CI	ACNGT	5 cut(s)	11, 543, 556, 913, 1393
Bst6I	CTCTTC	5 cut(s)	81, 516, 1302, 1461, 1827
BstBAI	YACGTR	1 cut(s)	356
BstC8I	GCNNGC	5 cut(s)	56, 237, 327, 993, 1293
BstDEI	CTNAG	8 cut(s)	36, 348, 868, 975, 996, 1135, 1452, 1509
BstDSI	CCRYGG	2 cut(s)	320, 1286
BstFNI	CGCG	1 cut(s)	576
BstKTI	GATC	8 cut(s)	124, 264, 1057, 1105, 1200, 1639, 1705, 1771
BstMAI	GTCTC	6 cut(s)	215, 549, 568, 619, 647, 1418
BstMBI	GATC	8 cut(s)	121, 261, 1054, 1102, 1197, 1636, 1702, 1768
BstMWI	GCNNNNNNNGC	3 cut(s)	32, 992, 1155
BstNI	CCWGG	1 cut(s)	560
BstNSI	RCATGY	1 cut(s)	802
BstSCI	CCNGG	2 cut(s)	558, 1692
BstSFI	CTRYAG	1 cut(s)	27
BstSLI	GKGCMC	1 cut(s)	1151
BstUI	CGCG	1 cut(s)	576
BstV1I	GCAGC	1 cut(s)	539
BstX2I	RGATCY	3 cut(s)	1102, 1702, 1768
BstYI	RGATCY	3 cut(s)	1102, 1702, 1768
BsuI	GTATCC	1 cut(s)	384
BsuRI	GGCC	1 cut(s)	1507
BtgI	CCRYGG	2 cut(s)	320, 1286
BtgZI	GCGATG	1 cut(s)	1872
BtsIMutI	CAGTG	3 cut(s)	445, 1141, 1449
Cac8I	GCNNGC	5 cut(s)	56, 237, 327, 993, 1293
CaiI	CAGNNNCTG	1 cut(s)	547
Cfr13I	GGNCC	4 cut(s)	188, 1249, 1345, 1376
Csp6I	GTAC	1 cut(s)	304
CspCI	CAANNNNNGTGG	2 cut(s)	678, 713
CviQI	GTAC	1 cut(s)	304
DdeI	CTNAG	8 cut(s)	36, 348, 868, 975, 996, 1135, 1452, 1509
DpnI	GATC	8 cut(s)	123, 263, 1056, 1104, 1199, 1638, 1704, 1770
DpnII	GATC	8 cut(s)	121, 261, 1054, 1102, 1197, 1636, 1702, 1768
Eam1104I	CTCTTC	5 cut(s)	81, 516, 1302, 1461, 1827
EarI	CTCTTC	5 cut(s)	81, 516, 1302, 1461, 1827
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	3 cut(s)	40, 865, 991
Eco130I	CCWWGG	3 cut(s)	803, 1286, 1348
Eco24I	GRGCYC	4 cut(s)	42, 867, 993, 1839
Eco31I	GGTCTC	3 cut(s)	215, 568, 647
Eco47I	GGWCC	4 cut(s)	188, 1249, 1345, 1376
Eco53kI	GAGCTC	3 cut(s)	40, 865, 991
Eco57I	CTGAAG	1 cut(s)	435
Eco72I	CACGTG	1 cut(s)	356
EcoICRI	GAGCTC	3 cut(s)	40, 865, 991
EcoRI	GAATTC	1 cut(s)	680
EcoRII	CCWGG	1 cut(s)	558
EcoT14I	CCWWGG	3 cut(s)	803, 1286, 1348
EcoT22I	ATGCAT	2 cut(s)	677, 1219
EcoT38I	GRGCYC	4 cut(s)	42, 867, 993, 1839
ErhI	CCWWGG	3 cut(s)	803, 1286, 1348
Esp3I	CGTCTC	1 cut(s)	1418
FalI	AAGNNNNNCTT	2 cut(s)	1310, 1342
FaqI	GGGAC	2 cut(s)	110, 1262
FauNDI	CATATG	1 cut(s)	1574
FbaI	TGATCA	1 cut(s)	121
FblI	GTMKAC	1 cut(s)	659
Fnu4HI	GCNGC	3 cut(s)	33, 528, 1460
FriOI	GRGCYC	4 cut(s)	42, 867, 993, 1839
Fsp4HI	GCNGC	3 cut(s)	33, 528, 1460
FspBI	CTAG	2 cut(s)	51, 1239
GluI	GCNGC	3 cut(s)	33, 528, 1460
GsaI	CCCAGC	1 cut(s)	161
GsuI	CTGGAG	2 cut(s)	1052, 1679
HaeIII	GGCC	1 cut(s)	1507
HapII	CCGG	6 cut(s)	21, 89, 1094, 1694, 1760, 1826
HincII	GTYRAC	1 cut(s)	197
HindII	GTYRAC	1 cut(s)	197
HindIII	AAGCTT	1 cut(s)	1878
HpaII	CCGG	6 cut(s)	21, 89, 1094, 1694, 1760, 1826
HphI	GGTGA	7 cut(s)	23, 131, 196, 271, 394, 1319, 1874
Hpy166II	GTNNAC	3 cut(s)	197, 572, 660
Hpy188I	TCNGA	7 cut(s)	343, 349, 415, 549, 595, 619, 1102
Hpy8I	GTNNAC	3 cut(s)	197, 572, 660
HpyAV	CCTTC	8 cut(s)	139, 413, 598, 620, 817, 976, 1729, 1838
HpyCH4III	ACNGT	5 cut(s)	11, 543, 556, 913, 1393
HpyCH4IV	ACGT	3 cut(s)	199, 355, 1411
HpyF10VI	GCNNNNNNNGC	3 cut(s)	32, 992, 1155
HpyF3I	CTNAG	8 cut(s)	36, 348, 868, 975, 996, 1135, 1452, 1509
HpySE526I	ACGT	3 cut(s)	199, 355, 1411
Ksp22I	TGATCA	1 cut(s)	121
Kzo9I	GATC	8 cut(s)	121, 261, 1054, 1102, 1197, 1636, 1702, 1768
LguI	GCTCTTC	2 cut(s)	1302, 1827
LmnI	GCTCC	5 cut(s)	244, 467, 1430, 1456, 1537
Lsp1109I	GCAGC	1 cut(s)	539
LweI	GCATC	9 cut(s)	185, 306, 599, 1204, 1331, 1362, 1675, 1741, 1807
MaeI	CTAG	2 cut(s)	51, 1239
MaeII	ACGT	3 cut(s)	199, 355, 1411
MaeIII	GTNAC	4 cut(s)	1241, 1307, 1393, 1628
MalI	GATC	8 cut(s)	123, 263, 1056, 1104, 1199, 1638, 1704, 1770
MbiI	CCGCTC	1 cut(s)	1459
MboI	GATC	8 cut(s)	121, 261, 1054, 1102, 1197, 1636, 1702, 1768
MflI	RGATCY	3 cut(s)	1102, 1702, 1768
MhlI	GDGCHC	8 cut(s)	42, 237, 867, 993, 1151, 1554, 1657, 1839
MluCI	AATT	7 cut(s)	330, 506, 585, 680, 980, 1038, 1481
MlyI	GAGTC	5 cut(s)	67, 299, 1043, 1304, 1339
Mph1103I	ATGCAT	2 cut(s)	677, 1219
MseI	TTAA	3 cut(s)	129, 1485, 1882
MslI	CAYNNNNRTG	4 cut(s)	181, 921, 1144, 1358
MspA1I	CMGCKG	2 cut(s)	35, 275
MspI	CCGG	6 cut(s)	21, 89, 1094, 1694, 1760, 1826
MspR9I	CCNGG	2 cut(s)	560, 1694
Mva1269I	GAATGC	3 cut(s)	439, 1287, 1528
MvaI	CCWGG	1 cut(s)	560
MvnI	CGCG	1 cut(s)	576
MwoI	GCNNNNNNNGC	3 cut(s)	32, 992, 1155
NciI	CCSGG	1 cut(s)	1694
NcoI	CCATGG	1 cut(s)	1286
NdeI	CATATG	1 cut(s)	1574
NdeII	GATC	8 cut(s)	121, 261, 1054, 1102, 1197, 1636, 1702, 1768
NlaIV	GGNNCC	6 cut(s)	609, 1148, 1250, 1675, 1741, 1807
NmuCI	GTSAC	4 cut(s)	1241, 1307, 1393, 1628
NsiI	ATGCAT	2 cut(s)	677, 1219
NspI	RCATGY	1 cut(s)	802
OliI	CACNNNNGTG	1 cut(s)	1144
PciSI	GCTCTTC	2 cut(s)	1302, 1827
PctI	GAATGC	3 cut(s)	439, 1287, 1528
PfeI	GAWTC	5 cut(s)	17, 344, 1083, 1090, 1338
PkrI	GCNGC	3 cut(s)	34, 529, 1461
PleI	GAGTC	5 cut(s)	67, 298, 1042, 1304, 1338
PmaCI	CACGTG	1 cut(s)	356
PmlI	CACGTG	1 cut(s)	356
PpsI	GAGTC	5 cut(s)	67, 298, 1042, 1304, 1338
Ppu21I	YACGTR	1 cut(s)	356
Psp124BI	GAGCTC	3 cut(s)	42, 867, 993
Psp1406I	AACGTT	1 cut(s)	199
Psp6I	CCWGG	1 cut(s)	558
PspCI	CACGTG	1 cut(s)	356
PspFI	CCCAGC	1 cut(s)	157
PspGI	CCWGG	1 cut(s)	558
PspN4I	GGNNCC	6 cut(s)	609, 1148, 1250, 1675, 1741, 1807
PspPI	GGNCC	4 cut(s)	188, 1249, 1345, 1376
PstNI	CAGNNNCTG	1 cut(s)	547
PsuI	RGATCY	3 cut(s)	1102, 1702, 1768
PvuII	CAGCTG	1 cut(s)	275
RsaI	GTAC	1 cut(s)	305
RsaNI	GTAC	1 cut(s)	304
RseI	CAYNNNNRTG	4 cut(s)	181, 921, 1144, 1358
SacI	GAGCTC	3 cut(s)	42, 867, 993
SapI	GCTCTTC	2 cut(s)	1302, 1827
SaqAI	TTAA	3 cut(s)	129, 1485, 1882
SatI	GCNGC	3 cut(s)	33, 528, 1460
Sau3AI	GATC	8 cut(s)	121, 261, 1054, 1102, 1197, 1636, 1702, 1768
Sau96I	GGNCC	4 cut(s)	188, 1249, 1345, 1376
SchI	GAGTC	5 cut(s)	67, 299, 1043, 1304, 1339
ScrFI	CCNGG	2 cut(s)	560, 1694
SduI	GDGCHC	8 cut(s)	42, 237, 867, 993, 1151, 1554, 1657, 1839
SfaNI	GCATC	9 cut(s)	185, 306, 599, 1204, 1331, 1362, 1675, 1741, 1807
SfcI	CTRYAG	1 cut(s)	27
SinI	GGWCC	4 cut(s)	188, 1249, 1345, 1376
SmiMI	CAYNNNNRTG	4 cut(s)	181, 921, 1144, 1358
SmlI	CTYRAG	2 cut(s)	1312, 1327
SmoI	CTYRAG	2 cut(s)	1312, 1327
Sse9I	AATT	7 cut(s)	330, 506, 585, 680, 980, 1038, 1481
SsiI	CCGC	4 cut(s)	5, 33, 186, 1459
SspMI	CTAG	2 cut(s)	51, 1239
SstI	GAGCTC	3 cut(s)	42, 867, 993
StyD4I	CCNGG	2 cut(s)	558, 1692
StyI	CCWWGG	3 cut(s)	803, 1286, 1348
TaaI	ACNGT	5 cut(s)	11, 543, 556, 913, 1393
TaiI	ACGT	3 cut(s)	202, 358, 1414
TaqI	TCGA	2 cut(s)	63, 255
TasI	AATT	7 cut(s)	330, 506, 585, 680, 980, 1038, 1481
TauI	GCSGC	2 cut(s)	35, 1462
TfiI	GAWTC	5 cut(s)	17, 344, 1083, 1090, 1338
Tru1I	TTAA	3 cut(s)	129, 1485, 1882
Tru9I	TTAA	3 cut(s)	129, 1485, 1882
TscAI	CASTG	3 cut(s)	445, 1148, 1456
TseFI	GTSAC	4 cut(s)	1241, 1307, 1393, 1628
TseI	GCWGC	1 cut(s)	527
Tsp45I	GTSAC	4 cut(s)	1241, 1307, 1393, 1628
TspDTI	ATGAA	4 cut(s)	783, 1222, 1415, 1561
TspGWI	ACGGA	2 cut(s)	324, 1095
TspRI	CASTG	3 cut(s)	445, 1148, 1456
VpaK11BI	GGWCC	4 cut(s)	188, 1249, 1345, 1376
XapI	RAATTY	3 cut(s)	680, 980, 1481
XceI	RCATGY	1 cut(s)	802
XcmI	CCANNNNNNNNNTGG	1 cut(s)	844
XmiI	GTMKAC	1 cut(s)	659
XspI	CTAG	2 cut(s)	51, 1239
Zsp2I	ATGCAT	2 cut(s)	677, 1219

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.