MD16G1094700.v1.1

Domain of unknown function DUF220

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001775
Physical Location & Seq
Forward (+)
6553999 .. 6556602
2604 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1094700.v1.1.491

Sequence Viewer

Length: 1005 bp
ATGGGTGAGACAGAGGTGACCACGACGAACGTAAACGTTGGCAAGAACGAGAGAAATGGGCAGCTCAAGGCCTTTGATCTGGCGTTTTCCAAAGCGTTGTTGCAGGTTCCTCAGAAGCTGCAAAATTGTGTAAAGTCGCAGCTAAGGAGACTGGTAAAGGATGACGGAGTGAAAACGGTGAGCTGTCTACCGAAAAAGGCTGCATCTTTGGAGCTTGACTTGGAGAAGCAGTTGCAAGCATGGAGAGAAAATCCTTCTTGGGTTGATCAACCACCAGATATGAAGGTCAGCGTACCAAAAGGTTCTCTTTGCAACCTCGGTGTAAGCGTTGATGTTGGGTTGCCCCCGGATGCAGTATATAATATTGTAACTGACCCTGATAATAAGAGGGTTTTCAAAAATATTAAGGAAGTGATATCCAGAAACGTTTTGGTCGATGAAGGTCATAGGCAGGTCGTTGAACTGGAGCAAGCTGCTATATGGAAGTTTCTTTGGTGGTCAGGGACCTTTGCGGTTCATGTTTTAGTAGATCAAAACAGAAAAGATCACTCAATGAAGTTCAAGCAAATCAAAACAGGGTTTATGGAGAAATTTGAAGGCTGCTGGAGAATGGAACCTCTTTTTGTCGATGAAAAGACCTGCTTTCCGTTCAAACCCAAGACATGGGCAGATTATAATTCTTGCACAGGAGGCAAAGGAAGGATCGGTTCAAAGTTGAGCTTGGAGCAACTGATCCAGCCATCTATAGTTCCACCCCCACCTATTTCTTGGTACCTACGGGGAATCACCAGCAAGACTACTGAGATGCTGATAAACGACATGCTTGCGGAAGCTGCCAGATTACGAGGAGAGTTAAATCCTGAAGAGTCTAATAAAGAGAACGAAATGTCTCAGGACAGGGAAGTACTTAGTCGCGTTGATAATGTATCTGACATCAAAGAAAGATGGGCTTTGCACAGGAGGAACGCAAAGCAATGCCATCGGAGGCTACCGCCTGTCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF220

Protein Analysis

335

Amino Acids

38.09

Weight (kDa)

9.04

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF220 PF02713 167 - 238 8.9e-32 Domain of unknown function DUF220
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 675
Acc36I ACCTGC 3 cut(s) 94, 442, 647
Acc65I GGTACC 1 cut(s) 771
AccB1I GGYRCC 1 cut(s) 771
AccB7I CCANNNNNTGG 1 cut(s) 663
AccI GTMKAC 1 cut(s) 187
AccII CGCG 1 cut(s) 915
AciI CCGC 3 cut(s) 512, 827, 992
AclI AACGTT 2 cut(s) 36, 426
AclWI GGATC 2 cut(s) 710, 727
AcsI RAATTY 1 cut(s) 590
AcuI CTGAAG 1 cut(s) 882
AfaI GTAC 3 cut(s) 294, 773, 906
AfiI CCNNNNNNNGG 1 cut(s) 663
AgsI TTSAA 6 cut(s) 397, 461, 562, 596, 652, 711
AluBI AGCT 8 cut(s) 64, 118, 142, 183, 214, 473, 720, 833
AluI AGCT 8 cut(s) 64, 118, 142, 183, 214, 473, 720, 833
Alw26I GTCTC 3 cut(s) 2, 142, 894
AlwI GGATC 2 cut(s) 710, 727
AlwNI CAGNNNCTG 1 cut(s) 118
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 7 cut(s) 61, 118, 139, 200, 473, 600, 833
ApoI RAATTY 1 cut(s) 590
Asp718I GGTACC 1 cut(s) 771
AspS9I GGNCC 1 cut(s) 504
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 4 cut(s) 17, 28, 190, 778
AvaII GGWCC 1 cut(s) 504
BanI GGYRCC 1 cut(s) 771
BbvI GCAGC 7 cut(s) 73, 105, 151, 187, 460, 587, 820
BccI CCATC 3 cut(s) 748, 939, 987
BcgI CGANNNNNNTGC 4 cut(s) 806, 840, 962, 996
BclI TGATCA 1 cut(s) 265
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 3 cut(s) 2, 142, 894
BfmI CTRYAG 1 cut(s) 744
BfuAI ACCTGC 3 cut(s) 94, 442, 647
BisI GCNGC 7 cut(s) 62, 119, 140, 201, 474, 601, 834
BlsI GCNGC 7 cut(s) 63, 120, 141, 202, 475, 602, 835
BmcAI AGTACT 1 cut(s) 906
Bme1390I CCNGG 1 cut(s) 347
Bme18I GGWCC 1 cut(s) 504
BmgT120I GGNCC 1 cut(s) 504
BmiI GGNNCC 4 cut(s) 108, 505, 615, 773
BmrFI CCNGG 1 cut(s) 347
BmsI GCATC 3 cut(s) 212, 340, 795
BpmI CTGGAG 2 cut(s) 485, 625
Bpu10I CCTNAGC 1 cut(s) 143
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 347
BsaJI CCNNGG 2 cut(s) 316, 345
BsaXI ACNNNNNCTCC 2 cut(s) 215, 245
Bsc4I CCNNNNNNNGG 1 cut(s) 663
Bse1I ACTGG 2 cut(s) 156, 468
Bse3DI GCAATG 1 cut(s) 980
BseDI CCNNGG 2 cut(s) 316, 345
BseGI GGATG 2 cut(s) 166, 355
BseLI CCNNNNNNNGG 1 cut(s) 663
BseMI GCAATG 1 cut(s) 980
BseMII CTCAG 3 cut(s) 125, 792, 905
BseNI ACTGG 2 cut(s) 156, 468
BseRI GAGGAG 1 cut(s) 861
BseXI GCAGC 7 cut(s) 73, 105, 151, 187, 460, 587, 820
Bsh1236I CGCG 1 cut(s) 915
BshFI GGCC 1 cut(s) 71
BshNI GGYRCC 1 cut(s) 771
BsiSI CCGG 1 cut(s) 347
BslFI GGGAC 1 cut(s) 517
BslI CCNNNNNNNGG 1 cut(s) 663
BsmAI GTCTC 3 cut(s) 2, 142, 894
BsmFI GGGAC 1 cut(s) 517
BsnI GGCC 1 cut(s) 71
Bsp143I GATC 6 cut(s) 76, 265, 529, 544, 702, 732
BspACI CCGC 3 cut(s) 512, 827, 992
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 3 cut(s) 124, 793, 904
BspFNI CGCG 1 cut(s) 915
BspLI GGNNCC 4 cut(s) 108, 505, 615, 773
BspMI ACCTGC 3 cut(s) 94, 442, 647
BspPI GGATC 2 cut(s) 710, 727
BspT107I GGYRCC 1 cut(s) 771
BsrDI GCAATG 1 cut(s) 980
BsrI ACTGG 2 cut(s) 156, 468
BssECI CCNNGG 2 cut(s) 316, 345
BssMI GATC 6 cut(s) 76, 265, 529, 544, 702, 732
Bst4CI ACNGT 1 cut(s) 178
Bst6I CTCTTC 1 cut(s) 858
BstC8I GCNNGC 3 cut(s) 237, 471, 825
BstDEI CTNAG 5 cut(s) 111, 143, 801, 891, 908
BstEII GGTNACC 1 cut(s) 16
BstF5I GGATG 2 cut(s) 166, 355
BstFNI CGCG 1 cut(s) 915
BstKTI GATC 6 cut(s) 79, 268, 532, 547, 705, 735
BstMAI GTCTC 3 cut(s) 2, 142, 894
BstMBI GATC 6 cut(s) 76, 265, 529, 544, 702, 732
BstMWI GCNNNNNNNGC 2 cut(s) 690, 833
BstNSI RCATGY 1 cut(s) 823
BstPI GGTNACC 1 cut(s) 16
BstSCI CCNGG 1 cut(s) 345
BstSFI CTRYAG 1 cut(s) 744
BstUI CGCG 1 cut(s) 915
BstV1I GCAGC 7 cut(s) 73, 105, 151, 187, 460, 587, 820
BsuRI GGCC 1 cut(s) 71
BtsCI GGATG 2 cut(s) 166, 355
BveI ACCTGC 3 cut(s) 94, 442, 647
Cac8I GCNNGC 3 cut(s) 237, 471, 825
CaiI CAGNNNCTG 1 cut(s) 118
Cfr13I GGNCC 1 cut(s) 504
Csp6I GTAC 3 cut(s) 293, 772, 905
CviAII CATG 4 cut(s) 240, 518, 663, 820
CviQI GTAC 3 cut(s) 293, 772, 905
DdeI CTNAG 5 cut(s) 111, 143, 801, 891, 908
DpnI GATC 6 cut(s) 78, 267, 531, 546, 704, 734
DpnII GATC 6 cut(s) 76, 265, 529, 544, 702, 732
Eam1104I CTCTTC 1 cut(s) 858
EarI CTCTTC 1 cut(s) 858
Eco147I AGGCCT 1 cut(s) 71
Eco32I GATATC 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 504
Eco57I CTGAAG 1 cut(s) 882
Eco91I GGTNACC 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 504
EcoO65I GGTNACC 1 cut(s) 16
EcoRV GATATC 1 cut(s) 417
FaeI CATG 4 cut(s) 243, 521, 666, 823
FalI AAGNNNNNCTT 8 cut(s) 291, 323, 626, 658, 704, 736, 934, 966
FaqI GGGAC 1 cut(s) 517
FatI CATG 4 cut(s) 239, 517, 662, 819
FbaI TGATCA 1 cut(s) 265
FblI GTMKAC 1 cut(s) 187
Fnu4HI GCNGC 7 cut(s) 62, 119, 140, 201, 474, 601, 834
FokI GGATG 2 cut(s) 173, 362
Fsp4HI GCNGC 7 cut(s) 62, 119, 140, 201, 474, 601, 834
GluI GCNGC 7 cut(s) 62, 119, 140, 201, 474, 601, 834
GsuI CTGGAG 2 cut(s) 485, 625
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 347
Hin1II CATG 4 cut(s) 243, 521, 666, 823
HinfI GANTC 2 cut(s) 783, 866
HpaII CCGG 1 cut(s) 347
HphI GGTGA 4 cut(s) 17, 28, 190, 778
Hpy166II GTNNAC 2 cut(s) 34, 188
Hpy188I TCNGA 3 cut(s) 114, 931, 984
Hpy188III TCNNGA 3 cut(s) 420, 860, 893
Hpy8I GTNNAC 2 cut(s) 34, 188
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 5 cut(s) 264, 277, 434, 590, 693
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4IV ACGT 3 cut(s) 30, 36, 426
HpyCH4V TGCA 8 cut(s) 103, 121, 203, 235, 312, 353, 684, 955
HpyF10VI GCNNNNNNNGC 2 cut(s) 690, 833
HpyF3I CTNAG 5 cut(s) 111, 143, 801, 891, 908
HpySE526I ACGT 3 cut(s) 30, 36, 426
Hsp92II CATG 4 cut(s) 243, 521, 666, 823
KpnI GGTACC 1 cut(s) 775
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 6 cut(s) 76, 265, 529, 544, 702, 732
LmnI GCTCC 3 cut(s) 211, 466, 724
Lsp1109I GCAGC 7 cut(s) 73, 105, 151, 187, 460, 587, 820
LweI GCATC 3 cut(s) 212, 340, 795
MaeII ACGT 3 cut(s) 30, 36, 426
MaeIII GTNAC 2 cut(s) 16, 367
MalI GATC 6 cut(s) 78, 267, 531, 546, 704, 734
MboI GATC 6 cut(s) 76, 265, 529, 544, 702, 732
MboII GAAGA 1 cut(s) 875
MluCI AATT 3 cut(s) 124, 590, 676
MlyI GAGTC 1 cut(s) 875
MnlI CCTC 9 cut(s) 7, 120, 326, 381, 627, 683, 839, 954, 978
MseI TTAA 2 cut(s) 405, 854
MspI CCGG 1 cut(s) 347
MspR9I CCNGG 1 cut(s) 347
MvnI CGCG 1 cut(s) 915
MwoI GCNNNNNNNGC 2 cut(s) 690, 833
NciI CCSGG 1 cut(s) 347
NdeII GATC 6 cut(s) 76, 265, 529, 544, 702, 732
NlaIII CATG 4 cut(s) 243, 521, 666, 823
NlaIV GGNNCC 4 cut(s) 108, 505, 615, 773
NmuCI GTSAC 1 cut(s) 16
NspI RCATGY 1 cut(s) 823
PceI AGGCCT 1 cut(s) 71
PcsI WCGNNNNNNNCGW 2 cut(s) 324, 432
PfeI GAWTC 1 cut(s) 783
PflMI CCANNNNNTGG 1 cut(s) 663
PkrI GCNGC 7 cut(s) 63, 120, 141, 202, 475, 602, 835
PleI GAGTC 1 cut(s) 874
PpsI GAGTC 1 cut(s) 874
PpuMI RGGWCCY 1 cut(s) 504
PsiI TTATAA 1 cut(s) 675
Psp1406I AACGTT 2 cut(s) 36, 426
Psp5II RGGWCCY 1 cut(s) 504
PspEI GGTNACC 1 cut(s) 16
PspN4I GGNNCC 4 cut(s) 108, 505, 615, 773
PspPI GGNCC 1 cut(s) 504
PspPPI RGGWCCY 1 cut(s) 504
PstNI CAGNNNCTG 1 cut(s) 118
RsaI GTAC 3 cut(s) 294, 773, 906
RsaNI GTAC 3 cut(s) 293, 772, 905
SaqAI TTAA 2 cut(s) 405, 854
SatI GCNGC 7 cut(s) 62, 119, 140, 201, 474, 601, 834
Sau3AI GATC 6 cut(s) 76, 265, 529, 544, 702, 732
Sau96I GGNCC 1 cut(s) 504
ScaI AGTACT 1 cut(s) 906
SchI GAGTC 1 cut(s) 875
ScrFI CCNGG 1 cut(s) 347
SfaNI GCATC 3 cut(s) 212, 340, 795
SfcI CTRYAG 1 cut(s) 744
SinI GGWCC 1 cut(s) 504
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
Sse9I AATT 3 cut(s) 124, 590, 676
SseBI AGGCCT 1 cut(s) 71
SsiI CCGC 3 cut(s) 512, 827, 992
SspI AATATT 2 cut(s) 364, 403
StuI AGGCCT 1 cut(s) 71
StyD4I CCNGG 1 cut(s) 345
TaaI ACNGT 1 cut(s) 178
TaiI ACGT 3 cut(s) 33, 39, 429
TaqI TCGA 2 cut(s) 435, 627
TasI AATT 3 cut(s) 124, 590, 676
TatI WGTACW 1 cut(s) 904
TfiI GAWTC 1 cut(s) 783
Tru1I TTAA 2 cut(s) 405, 854
Tru9I TTAA 2 cut(s) 405, 854
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 7 cut(s) 61, 118, 139, 200, 473, 600, 833
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 5 cut(s) 296, 453, 506, 569, 645
TspGWI ACGGA 2 cut(s) 180, 636
Van91I CCANNNNNTGG 1 cut(s) 663
VpaK11BI GGWCC 1 cut(s) 504
XapI RAATTY 1 cut(s) 590
XceI RCATGY 1 cut(s) 823
XcmI CCANNNNNNNNNTGG 2 cut(s) 427, 765
XmiI GTMKAC 1 cut(s) 187
ZrmI AGTACT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.