Rh4CG431900

Domain of unknown function DUF220

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001775
Physical Location & Seq
Reverse (-)
67203461 .. 67206123
2663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG431900.1

Sequence Viewer

Length: 873 bp
ATGTGTCAGTCACAGTTTAGAAGACTACCAAAAGCTGATGGAGTTAAAACAGTGAGCTCTGTCCCTAAAAAGGCTTCATCTTTGGAGGTTGACCTGGAAAAGCAGATGCAAGCATGGAGAGAAAATCCTTCATGGGATGATCAAACACCACATATAAAGGTCAGTGTGCCAAAAGGTTCTCTCTGCAACCTCAGTGTAAACGTCAATGTTGGGTTGCCCCCAGATGCAGTATATAATATTGTAACTGACCCCGACAATAAGAGGGTTTTCAAGAATATTAAGGAGGTGATATCCAGAAGAGTTTTGGTTGACGAAGGTCTTAGGCAGGTTGTTGATCTAGAGCAAGCAGCTATATGGAAATTTCTTTGGTGGTCAGGGACCATTGCAGTTCATGTTTTAGTAGATCAAAACAGAGAAGACCACTCAATGAAGTTCAAGCAAGTCAAAACAGGGTTTATGGAGAGATTTGAAGGCTGCTGGAGAGTGGAGCCTCTTTTTGTTGATGAAAAAACCTGCTTTCCCTATAAACCCACAACATGGGCAGACTATAATTCACACACTGGAGGCAAAGGAAGGATCGGATCAAAGGTGAGCTTGGACCAACTGATCCAACCGTCTATTGTTCCTCCCCCACCCATTTCTTGGTATCTGAGGGGTATCACCGCCAAGACCACAGAGATGTTGATAAATGATCTTCTTGCTGAAGCCGCCAGAATACGGGGAGAATTAAACCCTGAGAAGTCTGATAAAGAGGTTGAAATGTCTGAGGAAGTACTTAATCAAGTTGATAACACCTGTAACATCAAAGAAAGATGGGCTATGCATAGGAGAAACGCAAAGCAATGTCATCGGAGGCTACCGACTGCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF220

Protein Analysis

290

Amino Acids

33.21

Weight (kDa)

8.94

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF220 PF02713 125 - 196 3.8e-29 Domain of unknown function DUF220
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 316, 521
AccB7I CCANNNNNTGG 2 cut(s) 537, 642
AciI CCGC 2 cut(s) 663, 708
AclWI GGATC 3 cut(s) 584, 589, 601
AcsI RAATTY 1 cut(s) 359
AcuI CTGAAG 1 cut(s) 723
AfaI GTAC 1 cut(s) 774
AfiI CCNNNNNNNGG 4 cut(s) 70, 537, 642, 717
AgsI TTSAA 4 cut(s) 271, 436, 470, 758
AjnI CCWGG 1 cut(s) 93
AluBI AGCT 4 cut(s) 35, 57, 350, 594
AluI AGCT 4 cut(s) 35, 57, 350, 594
Alw21I GWGCWC 1 cut(s) 59
AlwI GGATC 3 cut(s) 584, 589, 601
ApeKI GCWGC 2 cut(s) 347, 474
ApoI RAATTY 1 cut(s) 359
AspS9I GGNCC 2 cut(s) 378, 598
AsuHPI GGTGA 3 cut(s) 298, 601, 652
AvaII GGWCC 2 cut(s) 378, 598
BanII GRGCYC 1 cut(s) 59
BbsI GAAGAC 2 cut(s) 28, 423
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 2 cut(s) 359, 461
BccI CCATC 2 cut(s) 32, 807
BcgI CGANNNNNNTGC 2 cut(s) 830, 864
BciT130I CCWGG 1 cut(s) 95
BclI TGATCA 1 cut(s) 139
BfaI CTAG 1 cut(s) 338
BfuAI ACCTGC 2 cut(s) 316, 521
BisI GCNGC 3 cut(s) 348, 475, 708
BlsI GCNGC 3 cut(s) 349, 476, 709
BmcAI AGTACT 1 cut(s) 774
Bme1390I CCNGG 1 cut(s) 95
Bme18I GGWCC 2 cut(s) 378, 598
BmgT120I GGNCC 2 cut(s) 378, 598
BmiI GGNNCC 2 cut(s) 379, 489
BmrFI CCNGG 1 cut(s) 95
BmsI GCATC 2 cut(s) 96, 214
BoxI GACNNNNGTC 1 cut(s) 315
BpiI GAAGAC 2 cut(s) 28, 423
BpmI CTGGAG 2 cut(s) 499, 582
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 537, 642, 717
Bse1I ACTGG 1 cut(s) 565
Bse3DI GCAATG 2 cut(s) 381, 848
BseBI CCWGG 1 cut(s) 95
BseGI GGATG 1 cut(s) 142
BseLI CCNNNNNNNGG 4 cut(s) 70, 537, 642, 717
BseMI GCAATG 2 cut(s) 381, 848
BseMII CTCAG 4 cut(s) 205, 641, 726, 756
BseNI ACTGG 1 cut(s) 565
BseXI GCAGC 2 cut(s) 359, 461
BsiHKAI GWGCWC 1 cut(s) 59
BslFI GGGAC 2 cut(s) 47, 391
BslI CCNNNNNNNGG 4 cut(s) 70, 537, 642, 717
BsmFI GGGAC 2 cut(s) 47, 391
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 7 cut(s) 139, 334, 403, 576, 581, 606, 691
BspACI CCGC 2 cut(s) 663, 708
BspCNI CTCAG 4 cut(s) 204, 642, 727, 757
BspLI GGNNCC 2 cut(s) 379, 489
BspMI ACCTGC 2 cut(s) 316, 521
BspPI GGATC 3 cut(s) 584, 589, 601
BsrDI GCAATG 2 cut(s) 381, 848
BsrI ACTGG 1 cut(s) 565
BssMI GATC 7 cut(s) 139, 334, 403, 576, 581, 606, 691
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 3 cut(s) 15, 52, 615
Bst6I CTCTTC 1 cut(s) 292
BstC8I GCNNGC 2 cut(s) 111, 345
BstDEI CTNAG 5 cut(s) 191, 320, 650, 735, 765
BstF5I GGATG 1 cut(s) 142
BstKTI GATC 7 cut(s) 142, 337, 406, 579, 584, 609, 694
BstMBI GATC 7 cut(s) 139, 334, 403, 576, 581, 606, 691
BstMWI GCNNNNNNNGC 1 cut(s) 707
BstNI CCWGG 1 cut(s) 95
BstPAI GACNNNNGTC 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 359, 461
BstV2I GAAGAC 2 cut(s) 28, 423
BtsCI GGATG 1 cut(s) 142
BtsIMutI CAGTG 4 cut(s) 57, 169, 199, 558
BveI ACCTGC 2 cut(s) 316, 521
Cac8I GCNNGC 2 cut(s) 111, 345
Cfr13I GGNCC 2 cut(s) 378, 598
Csp6I GTAC 1 cut(s) 773
CviAII CATG 4 cut(s) 114, 132, 392, 537
CviQI GTAC 1 cut(s) 773
DdeI CTNAG 5 cut(s) 191, 320, 650, 735, 765
DpnI GATC 7 cut(s) 141, 336, 405, 578, 583, 608, 693
DpnII GATC 7 cut(s) 139, 334, 403, 576, 581, 606, 691
Eam1104I CTCTTC 1 cut(s) 292
EarI CTCTTC 1 cut(s) 292
Ecl136II GAGCTC 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 59
Eco32I GATATC 1 cut(s) 291
Eco47I GGWCC 2 cut(s) 378, 598
Eco53kI GAGCTC 1 cut(s) 57
Eco57I CTGAAG 1 cut(s) 723
EcoICRI GAGCTC 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 93
EcoRV GATATC 1 cut(s) 291
EcoT22I ATGCAT 1 cut(s) 825
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 4 cut(s) 117, 135, 395, 540
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 2 cut(s) 47, 391
FatI CATG 4 cut(s) 113, 131, 391, 536
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 3 cut(s) 348, 475, 708
FokI GGATG 1 cut(s) 149
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 3 cut(s) 348, 475, 708
FspBI CTAG 1 cut(s) 338
GluI GCNGC 3 cut(s) 348, 475, 708
GsuI CTGGAG 2 cut(s) 499, 582
Hin1II CATG 4 cut(s) 117, 135, 395, 540
HincII GTYRAC 2 cut(s) 91, 310
HindII GTYRAC 2 cut(s) 91, 310
HphI GGTGA 3 cut(s) 298, 601, 652
Hpy166II GTNNAC 3 cut(s) 91, 199, 310
Hpy188I TCNGA 5 cut(s) 581, 651, 745, 766, 852
Hpy188III TCNNGA 3 cut(s) 271, 294, 338
Hpy8I GTNNAC 3 cut(s) 91, 199, 310
HpyAV CCTTC 4 cut(s) 138, 308, 464, 567
HpyCH4III ACNGT 3 cut(s) 15, 52, 615
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 5 cut(s) 109, 186, 227, 386, 823
HpyF10VI GCNNNNNNNGC 1 cut(s) 707
HpyF3I CTNAG 5 cut(s) 191, 320, 650, 735, 765
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 4 cut(s) 117, 135, 395, 540
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 7 cut(s) 139, 334, 403, 576, 581, 606, 691
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 2 cut(s) 359, 461
LweI GCATC 2 cut(s) 96, 214
MaeI CTAG 1 cut(s) 338
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 3 cut(s) 9, 241, 797
MalI GATC 7 cut(s) 141, 336, 405, 578, 583, 608, 693
MboI GATC 7 cut(s) 139, 334, 403, 576, 581, 606, 691
MboII GAAGA 4 cut(s) 33, 309, 428, 686
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 3 cut(s) 359, 550, 725
MmeI TCCRAC 1 cut(s) 634
Mph1103I ATGCAT 1 cut(s) 825
MseI TTAA 4 cut(s) 45, 279, 728, 777
MslI CAYNNNNRTG 1 cut(s) 677
MspR9I CCNGG 1 cut(s) 95
MvaI CCWGG 1 cut(s) 95
MwoI GCNNNNNNNGC 1 cut(s) 707
NdeII GATC 7 cut(s) 139, 334, 403, 576, 581, 606, 691
NlaIII CATG 4 cut(s) 117, 135, 395, 540
NlaIV GGNNCC 2 cut(s) 379, 489
NmuCI GTSAC 1 cut(s) 9
NsiI ATGCAT 1 cut(s) 825
PflMI CCANNNNNTGG 2 cut(s) 537, 642
PkrI GCNGC 3 cut(s) 349, 476, 709
PshAI GACNNNNGTC 1 cut(s) 315
Psp124BI GAGCTC 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 93
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 2 cut(s) 379, 489
PspPI GGNCC 2 cut(s) 378, 598
RsaI GTAC 1 cut(s) 774
RsaNI GTAC 1 cut(s) 773
RseI CAYNNNNRTG 1 cut(s) 677
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 4 cut(s) 45, 279, 728, 777
SatI GCNGC 3 cut(s) 348, 475, 708
Sau3AI GATC 7 cut(s) 139, 334, 403, 576, 581, 606, 691
Sau96I GGNCC 2 cut(s) 378, 598
ScaI AGTACT 1 cut(s) 774
ScrFI CCNGG 1 cut(s) 95
SduI GDGCHC 1 cut(s) 59
SfaNI GCATC 2 cut(s) 96, 214
SinI GGWCC 2 cut(s) 378, 598
SmiMI CAYNNNNRTG 1 cut(s) 677
Sse9I AATT 3 cut(s) 359, 550, 725
SsiI CCGC 2 cut(s) 663, 708
SspI AATATT 2 cut(s) 238, 277
SspMI CTAG 1 cut(s) 338
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 93
TaaI ACNGT 3 cut(s) 15, 52, 615
TaiI ACGT 1 cut(s) 204
TasI AATT 3 cut(s) 359, 550, 725
TatI WGTACW 1 cut(s) 772
TauI GCSGC 1 cut(s) 710
Tru1I TTAA 4 cut(s) 45, 279, 728, 777
Tru9I TTAA 4 cut(s) 45, 279, 728, 777
TscAI CASTG 4 cut(s) 57, 169, 199, 565
TseFI GTSAC 1 cut(s) 9
TseI GCWGC 2 cut(s) 347, 474
Tsp45I GTSAC 1 cut(s) 9
TspDTI ATGAA 5 cut(s) 66, 120, 380, 443, 519
TspRI CASTG 4 cut(s) 57, 169, 199, 565
Van91I CCANNNNNTGG 2 cut(s) 537, 642
VpaK11BI GGWCC 2 cut(s) 378, 598
XapI RAATTY 1 cut(s) 359
XbaI TCTAGA 1 cut(s) 337
XcmI CCANNNNNNNNNTGG 2 cut(s) 301, 639
XspI CTAG 1 cut(s) 338
ZrmI AGTACT 1 cut(s) 774
Zsp2I ATGCAT 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.