Rh4DG413000

Domain of unknown function DUF220

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001775
Physical Location & Seq
Reverse (-)
62393614 .. 62397146
3533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG413000.1

Sequence Viewer

Length: 1029 bp
ATGATGCCATATTTGCTCTTTGCAGTAGTGATGGGTGAGACAGAGGTGCCCACGACAAGCATAGACATCCGGAAGAACGAGAAAAATGGGCGCCTCAAGGCATTGGATGTAGTGCTCTCTAAAGCTTTTGTCCAGGTTCCTCACAAGCTACAGAATTTTCTTAAGTCACAGTTTAGAAGACTACCAAAAGCTGATGGAGTTAAAACAGTGAGCTCTGTCCCTAAAAAGGCTTCATCTTTGGAGGTTGACCTGGAAAAGCAGATGCAAGCATGGAGAGAAAATCCTTCATGGGATGATCAAACACCACATATAAAGGTCAGTGTGCCAAAAGGTTCTCTCTGCAACCTCAGTGTAAACGTCAATGTTGGGTTGCCCCCAGATGCAGTATATAATATTGTAACTGACCCCGACAATAAGAGGGTTTTCAAGAATATTAAGGAGGTGATATCCAGAAGAGTTTTGGTTGACGAAGGTCTTAGGCAGGTTGTTGATCTAGAGCAAGCAGCTATATGGAAATTTCTTTGGTGGTCAGGGACCATTGCAGTTCATGTTTTAGTAGATCAAAACAGAGAAGACCACTCGATGAAGTTCAAGCAAGTCAAAACAGGGTTTATGGAGAGATTTGAAGGCTGCTGGAGAGTGGAGCCTCTTTTTGTTGATGAAAAAACCTGCTTTCCCTATAAACCCACAACATGGGCAGACTATAATTCACACACTGGAGGCAAAGGAAGGATCGGATCAAAGGTGAGCTTGGACCAACTGATCCAACCGTCTATTGTTCCTCCCCCACCCATTTCTTGGTATCTGAGGGGTATCACCGCCAAGACCACAGAGATGTTGATAAATGATCTTCTTGCTGAAGCCGCCAGAATACGGGGAGAATTAAACCCTGAGAAGTCTGATAAAGAGGTTGAAATGTCTGAGGAAGTACTTAATCAAGTTGATAACACCTGTAACATCAAAGAAAGATGGGCTATGCATAGGAGAAATGCAAAGCAATGTCATCGGAGGCTACCGACTGCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF220

Protein Analysis

342

Amino Acids

39.11

Weight (kDa)

9.22

Isoelectric Point (pI)

38.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF220 PF02713 177 - 248 5.1e-29 Domain of unknown function DUF220
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 472, 677
AccB1I GGYRCC 2 cut(s) 46, 90
AccB7I CCANNNNNTGG 2 cut(s) 693, 798
AccIII TCCGGA 1 cut(s) 69
AciI CCGC 2 cut(s) 819, 864
AclWI GGATC 3 cut(s) 740, 745, 757
AcsI RAATTY 2 cut(s) 154, 515
AcuI CTGAAG 1 cut(s) 879
AcyI GRCGYC 1 cut(s) 91
AfaI GTAC 1 cut(s) 930
AfiI CCNNNNNNNGG 4 cut(s) 226, 693, 798, 873
AflII CTTAAG 1 cut(s) 161
AgsI TTSAA 4 cut(s) 427, 592, 626, 914
AjnI CCWGG 2 cut(s) 132, 249
AluBI AGCT 6 cut(s) 125, 148, 191, 213, 506, 750
AluI AGCT 6 cut(s) 125, 148, 191, 213, 506, 750
Alw21I GWGCWC 2 cut(s) 117, 215
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 3 cut(s) 740, 745, 757
Aor13HI TCCGGA 1 cut(s) 69
ApeKI GCWGC 2 cut(s) 503, 630
ApoI RAATTY 2 cut(s) 154, 515
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 2 cut(s) 534, 754
AsuHPI GGTGA 4 cut(s) 47, 454, 757, 808
AvaII GGWCC 2 cut(s) 534, 754
BaeGI GKGCMC 1 cut(s) 51
BanI GGYRCC 2 cut(s) 46, 90
BanII GRGCYC 1 cut(s) 215
BbsI GAAGAC 2 cut(s) 184, 579
Bbv12I GWGCWC 2 cut(s) 117, 215
BbvI GCAGC 2 cut(s) 515, 617
BccI CCATC 3 cut(s) 25, 188, 963
BcgI CGANNNNNNTGC 2 cut(s) 986, 1020
BciT130I CCWGG 2 cut(s) 134, 251
BclI TGATCA 1 cut(s) 295
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 1 cut(s) 494
BfmI CTRYAG 1 cut(s) 149
BfoI RGCGCY 1 cut(s) 94
BfrI CTTAAG 1 cut(s) 161
BfuAI ACCTGC 2 cut(s) 472, 677
BisI GCNGC 3 cut(s) 504, 631, 864
BlsI GCNGC 3 cut(s) 505, 632, 865
BmcAI AGTACT 1 cut(s) 930
Bme1390I CCNGG 2 cut(s) 134, 251
Bme18I GGWCC 2 cut(s) 534, 754
BmgT120I GGNCC 2 cut(s) 534, 754
BmiI GGNNCC 5 cut(s) 48, 92, 138, 535, 645
BmrFI CCNGG 2 cut(s) 134, 251
BmsI GCATC 2 cut(s) 252, 370
BoxI GACNNNNGTC 1 cut(s) 471
BpiI GAAGAC 2 cut(s) 184, 579
BpmI CTGGAG 2 cut(s) 655, 738
BpuEI CTTGAG 1 cut(s) 80
BsaHI GRCGYC 1 cut(s) 91
BsaWI WCCGGW 1 cut(s) 69
Bsc4I CCNNNNNNNGG 4 cut(s) 226, 693, 798, 873
Bse1I ACTGG 1 cut(s) 721
Bse3DI GCAATG 2 cut(s) 537, 1004
BseAI TCCGGA 1 cut(s) 69
BseBI CCWGG 2 cut(s) 134, 251
BseGI GGATG 3 cut(s) 66, 112, 298
BseLI CCNNNNNNNGG 4 cut(s) 226, 693, 798, 873
BseMI GCAATG 2 cut(s) 537, 1004
BseMII CTCAG 4 cut(s) 361, 797, 882, 912
BseNI ACTGG 1 cut(s) 721
BseSI GKGCMC 1 cut(s) 51
BseXI GCAGC 2 cut(s) 515, 617
BshNI GGYRCC 2 cut(s) 46, 90
BsiHKAI GWGCWC 2 cut(s) 117, 215
BsiSI CCGG 1 cut(s) 70
BslFI GGGAC 2 cut(s) 203, 547
BslI CCNNNNNNNGG 4 cut(s) 226, 693, 798, 873
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 203, 547
Bsp1286I GDGCHC 3 cut(s) 51, 117, 215
Bsp13I TCCGGA 1 cut(s) 69
Bsp143I GATC 7 cut(s) 295, 490, 559, 732, 737, 762, 847
BspACI CCGC 2 cut(s) 819, 864
BspCNI CTCAG 4 cut(s) 360, 798, 883, 913
BspEI TCCGGA 1 cut(s) 69
BspLI GGNNCC 5 cut(s) 48, 92, 138, 535, 645
BspMI ACCTGC 2 cut(s) 472, 677
BspPI GGATC 3 cut(s) 740, 745, 757
BspT107I GGYRCC 2 cut(s) 46, 90
BspTI CTTAAG 1 cut(s) 161
BsrDI GCAATG 2 cut(s) 537, 1004
BsrI ACTGG 1 cut(s) 721
BssMI GATC 7 cut(s) 295, 490, 559, 732, 737, 762, 847
BssNI GRCGYC 1 cut(s) 91
Bst2UI CCWGG 2 cut(s) 134, 251
Bst4CI ACNGT 3 cut(s) 171, 208, 771
Bst6I CTCTTC 1 cut(s) 448
BstACI GRCGYC 1 cut(s) 91
BstAFI CTTAAG 1 cut(s) 161
BstC8I GCNNGC 2 cut(s) 267, 501
BstDEI CTNAG 5 cut(s) 347, 476, 806, 891, 921
BstF5I GGATG 3 cut(s) 66, 112, 298
BstH2I RGCGCY 1 cut(s) 94
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 7 cut(s) 298, 493, 562, 735, 740, 765, 850
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 7 cut(s) 295, 490, 559, 732, 737, 762, 847
BstMWI GCNNNNNNNGC 2 cut(s) 13, 863
BstNI CCWGG 2 cut(s) 134, 251
BstPAI GACNNNNGTC 1 cut(s) 471
BstSCI CCNGG 2 cut(s) 132, 249
BstSFI CTRYAG 1 cut(s) 149
BstSLI GKGCMC 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 515, 617
BstV2I GAAGAC 2 cut(s) 184, 579
BtsCI GGATG 3 cut(s) 66, 112, 298
BtsIMutI CAGTG 4 cut(s) 213, 325, 355, 714
BveI ACCTGC 2 cut(s) 472, 677
Cac8I GCNNGC 2 cut(s) 267, 501
CfoI GCGC 1 cut(s) 93
Cfr13I GGNCC 2 cut(s) 534, 754
Csp6I GTAC 1 cut(s) 929
CviAII CATG 4 cut(s) 270, 288, 548, 693
CviQI GTAC 1 cut(s) 929
DdeI CTNAG 5 cut(s) 347, 476, 806, 891, 921
DinI GGCGCC 1 cut(s) 92
DpnI GATC 7 cut(s) 297, 492, 561, 734, 739, 764, 849
DpnII GATC 7 cut(s) 295, 490, 559, 732, 737, 762, 847
Eam1104I CTCTTC 1 cut(s) 448
EarI CTCTTC 1 cut(s) 448
Ecl136II GAGCTC 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 215
Eco32I GATATC 1 cut(s) 447
Eco47I GGWCC 2 cut(s) 534, 754
Eco53kI GAGCTC 1 cut(s) 213
Eco57I CTGAAG 1 cut(s) 879
EcoICRI GAGCTC 1 cut(s) 213
EcoRII CCWGG 2 cut(s) 132, 249
EcoRV GATATC 1 cut(s) 447
EcoT22I ATGCAT 1 cut(s) 981
EcoT38I GRGCYC 1 cut(s) 215
EgeI GGCGCC 1 cut(s) 92
EheI GGCGCC 1 cut(s) 92
FaeI CATG 4 cut(s) 273, 291, 551, 696
FalI AAGNNNNNCTT 2 cut(s) 734, 766
FaqI GGGAC 2 cut(s) 203, 547
FatI CATG 4 cut(s) 269, 287, 547, 692
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 3 cut(s) 504, 631, 864
FokI GGATG 3 cut(s) 53, 119, 305
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 3 cut(s) 504, 631, 864
FspBI CTAG 1 cut(s) 494
GlaI GCGC 1 cut(s) 92
GluI GCNGC 3 cut(s) 504, 631, 864
GsuI CTGGAG 2 cut(s) 655, 738
HaeII RGCGCY 1 cut(s) 94
HapII CCGG 1 cut(s) 70
HhaI GCGC 1 cut(s) 93
Hin1I GRCGYC 1 cut(s) 91
Hin1II CATG 4 cut(s) 273, 291, 551, 696
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HincII GTYRAC 2 cut(s) 247, 466
HindII GTYRAC 2 cut(s) 247, 466
HindIII AAGCTT 1 cut(s) 123
HpaII CCGG 1 cut(s) 70
HphI GGTGA 4 cut(s) 47, 454, 757, 808
Hpy166II GTNNAC 3 cut(s) 247, 355, 466
Hpy188I TCNGA 5 cut(s) 737, 807, 901, 922, 1008
Hpy188III TCNNGA 4 cut(s) 70, 427, 450, 494
Hpy8I GTNNAC 3 cut(s) 247, 355, 466
HpyAV CCTTC 4 cut(s) 294, 464, 620, 723
HpyCH4III ACNGT 3 cut(s) 171, 208, 771
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 7 cut(s) 23, 265, 342, 383, 542, 979, 992
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 863
HpyF3I CTNAG 5 cut(s) 347, 476, 806, 891, 921
HpySE526I ACGT 1 cut(s) 357
Hsp92I GRCGYC 1 cut(s) 91
Hsp92II CATG 4 cut(s) 273, 291, 551, 696
HspAI GCGC 1 cut(s) 91
KasI GGCGCC 1 cut(s) 90
Kpn2I TCCGGA 1 cut(s) 69
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 7 cut(s) 295, 490, 559, 732, 737, 762, 847
LmnI GCTCC 1 cut(s) 643
Lsp1109I GCAGC 2 cut(s) 515, 617
LweI GCATC 2 cut(s) 252, 370
MaeI CTAG 1 cut(s) 494
MaeII ACGT 1 cut(s) 357
MaeIII GTNAC 3 cut(s) 165, 397, 953
MalI GATC 7 cut(s) 297, 492, 561, 734, 739, 764, 849
MboI GATC 7 cut(s) 295, 490, 559, 732, 737, 762, 847
MboII GAAGA 5 cut(s) 85, 189, 465, 584, 842
MhlI GDGCHC 3 cut(s) 51, 117, 215
MluCI AATT 4 cut(s) 154, 515, 706, 881
Mly113I GGCGCC 1 cut(s) 91
MmeI TCCRAC 1 cut(s) 790
Mph1103I ATGCAT 1 cut(s) 981
MroI TCCGGA 1 cut(s) 69
MseI TTAA 5 cut(s) 162, 201, 435, 884, 933
MslI CAYNNNNRTG 1 cut(s) 833
MspCI CTTAAG 1 cut(s) 161
MspI CCGG 1 cut(s) 70
MspR9I CCNGG 2 cut(s) 134, 251
MvaI CCWGG 2 cut(s) 134, 251
MwoI GCNNNNNNNGC 2 cut(s) 13, 863
NarI GGCGCC 1 cut(s) 91
NdeII GATC 7 cut(s) 295, 490, 559, 732, 737, 762, 847
NlaIII CATG 4 cut(s) 273, 291, 551, 696
NlaIV GGNNCC 5 cut(s) 48, 92, 138, 535, 645
NmuCI GTSAC 1 cut(s) 165
NsiI ATGCAT 1 cut(s) 981
PflMI CCANNNNNTGG 2 cut(s) 693, 798
PkrI GCNGC 3 cut(s) 505, 632, 865
PluTI GGCGCC 1 cut(s) 94
PshAI GACNNNNGTC 1 cut(s) 471
Psp124BI GAGCTC 1 cut(s) 215
Psp6I CCWGG 2 cut(s) 132, 249
PspGI CCWGG 2 cut(s) 132, 249
PspN4I GGNNCC 5 cut(s) 48, 92, 138, 535, 645
PspPI GGNCC 2 cut(s) 534, 754
RsaI GTAC 1 cut(s) 930
RsaNI GTAC 1 cut(s) 929
RseI CAYNNNNRTG 1 cut(s) 833
SacI GAGCTC 1 cut(s) 215
SaqAI TTAA 5 cut(s) 162, 201, 435, 884, 933
SatI GCNGC 3 cut(s) 504, 631, 864
Sau3AI GATC 7 cut(s) 295, 490, 559, 732, 737, 762, 847
Sau96I GGNCC 2 cut(s) 534, 754
ScaI AGTACT 1 cut(s) 930
ScrFI CCNGG 2 cut(s) 134, 251
SduI GDGCHC 3 cut(s) 51, 117, 215
SfaNI GCATC 2 cut(s) 252, 370
SfcI CTRYAG 1 cut(s) 149
SfoI GGCGCC 1 cut(s) 92
SinI GGWCC 2 cut(s) 534, 754
SmiMI CAYNNNNRTG 1 cut(s) 833
SmlI CTYRAG 2 cut(s) 95, 161
SmoI CTYRAG 2 cut(s) 95, 161
Sse9I AATT 4 cut(s) 154, 515, 706, 881
SsiI CCGC 2 cut(s) 819, 864
SspDI GGCGCC 1 cut(s) 90
SspI AATATT 2 cut(s) 394, 433
SspMI CTAG 1 cut(s) 494
SstI GAGCTC 1 cut(s) 215
StyD4I CCNGG 2 cut(s) 132, 249
TaaI ACNGT 3 cut(s) 171, 208, 771
TaiI ACGT 1 cut(s) 360
TaqI TCGA 1 cut(s) 581
TasI AATT 4 cut(s) 154, 515, 706, 881
TatI WGTACW 1 cut(s) 928
TauI GCSGC 1 cut(s) 866
Tru1I TTAA 5 cut(s) 162, 201, 435, 884, 933
Tru9I TTAA 5 cut(s) 162, 201, 435, 884, 933
TscAI CASTG 4 cut(s) 213, 325, 355, 721
TseFI GTSAC 1 cut(s) 165
TseI GCWGC 2 cut(s) 503, 630
Tsp45I GTSAC 1 cut(s) 165
TspDTI ATGAA 5 cut(s) 222, 276, 536, 599, 675
TspRI CASTG 4 cut(s) 213, 325, 355, 721
Van91I CCANNNNNTGG 2 cut(s) 693, 798
Vha464I CTTAAG 1 cut(s) 161
VpaK11BI GGWCC 2 cut(s) 534, 754
XapI RAATTY 2 cut(s) 154, 515
XbaI TCTAGA 1 cut(s) 493
XcmI CCANNNNNNNNNTGG 2 cut(s) 457, 795
XspI CTAG 1 cut(s) 494
ZrmI AGTACT 1 cut(s) 930
Zsp2I ATGCAT 1 cut(s) 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.