Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0001775

Physical Location & Seq

Genomic Coordinates Forward (+)

5409612 .. 5411851

Gene Structure 2240 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom13g08130.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1701 bp

ATGGCTAACGGCGACGAAGACGGCGTCACTCACGACCTCCTTTCTATCCTCTCCTCCGCCGACAGAAACTTCCTCCTCCACAACAACGGCGGCCAGGCTGAGATCAGTAGTTTGACCGGAAAGATTGTGGGGTTGTATTTCTCCGCCTCGTGGTGCGGCCCGTGCCGCCGGTTCACCCCAAACTTGGTGGAGGTCCACCAGGACTTGGCCGCCAAAGGCGACTTTGAAGTTGTGTTCGTCTCTTCTGATAGAGATGAGGAATCGTTCACCGGATACTTCTCGAAAATGCCGTGGCTTTCGATTCCCTTTTCGGATTTGGAGACCCGAAAACGCCTCAAGGAGCTCTTCAAGGTCAGGGGGATTCCCCATCTTGTCATTATTGATGCCAATGGAAAAGTTTGTACTAATGATGGGACAAAAATTGTAATGGACCACGGGGCGGATGGTTATCCGTTCACCGCGGAGAAGATGAATTTATTAAAAGAGAAGGAAGCCGCGGTGAAGGAGAATCAGTCCTTGAGTTCTCTCTTGGTTTCTAGCTCCCGGGATTATTTGATTTCGAAGGATGGCAATAAGGTTTCTGTATCCGAGCTTGAAGGAAAGATTGTTGGGTTGTATTTCTCGCTTTATACTCACAAGCCATGCAAAGAATTTACTCAGGCACTTGTGAAGTTCCATGAGAAAATGAAGGAGAAAGGAGAGAACTTTGAGATTGTGTTGATATCTCTGGACCTTGAAGAGGAACACTTCAAAGAAGGGTTTCAAGTGTCATGGTTGGCATTGCCGTTTAAGGACAAGAACTGTGAAAAACTTGCTCGCTACTTTGATCTTGAGAATTTACCTACACTAGTTATAATTGGGCTAGATGGGAAGACCTTACACTCCAATGCGGCTAAACTCATTGAAGAGTATGGCGCTGAAGCCTACCCCTTTACACCAGAGAAGATTGCCGTGCTTGCTGAGTTGAAACTCGAAGAACAGACGATACAGTCACTTTTAGTTGCTGGGGGCAGAGACTTTGTGATTGAAAATAACGGTTCCAAGGTACCCATGTCTGAGCTTGCTGGGAAGCACATTCTGCTCTATTTCTCAGCTCATTGGTGCCCTCCATGTCGTGCATTCATGCCTAAGCTTATAAAAGCATACAATCAGATCAAGGCAAAGGACAATGCCATTGAAATAATCTTCCTCTCAAGCGATCGCGATCAACCCTCCTTCAAAGAACTATTTGCAACCATGCCTTGGTTAGCCATCCCATTAGGCGATCCAAGGAAGGCCCTCCTGCAGCACAAATTCAAAATCCAAGGCATTCCTGCATTGGTAGCCATCAGCCCCAACGGCCAGACACTCAGCACGCAGGCCCGGCAACTCATACAGGCGCATGGATCCGACGCTTATCCGTTCACAGAAGAGCATTTGAAGCAATTGGAGGAAAAGTTGGAGGAAGAAGCAAAGGGGTGGCCTGAGAAAGTGAAGAGTATACTCCATGCTGAACACGAGCTCACGCGTGTGCTCCACCACGACTATGTTTGCTGGTGTAGGGAGCCGGGAAGTGGCTGGTCTTTTTACTGCAAAGACTGCAACTTCGATCTCCACCCAAAATGTGCATTGAACAAGAGCAACAATGGAGGAACGGAGGATGACCCTCCCAAGGCAACGGAGGATTATATTTGTGACGGTGATGTGTGCCGTAAAGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0003824 GO:0004791 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006935 GO:0008150 GO:0008152 GO:0009605 GO:0009636 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010035 GO:0010038 GO:0010183 GO:0010769 GO:0015036 GO:0016043 GO:0016049 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0022414 GO:0022603 GO:0022604 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0040008 GO:0040011 GO:0042221 GO:0042330 GO:0043900 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0045595 GO:0046686 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050918 GO:0051128 GO:0051239 GO:0051510 GO:0051704 GO:0051716 GO:0055114 GO:0060284 GO:0060560 GO:0065007 GO:0070887 GO:0071840 GO:0080092 GO:0097237 GO:0098754 GO:0098869 GO:1990748 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C1_2 Thioredoxin_8

Protein Analysis

567

Amino Acids

63.61

Weight (kDa)

5.69

Isoelectric Point (pI)

33.51

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70480 AT1G70480
fragaria_vesca	FvH4_4g34241 FvH4_4g34250 FvH4_4g34260 FvH4_4g34260 FvH4_4g34260 FvH4_4g34260
malus_domestica	MD13G1093300.v1.1 MD13G1093500.v1.1 MD16G1094500.v1.1 MD16G1094600.v1.1 MD16G1094700.v1.1
prunus_persica	Prupe.1G251900_v2.0.a1 Prupe.1G252000_v2.0.a1
pyrus_communis	pycom13g08130 pycom13g08140 pycom16g08030 pycom16g08040 pycom16g08050
rosa_chinensis	RchiOBHm_Chr4g0443711 RchiOBHm_Chr6g0250421
rosa_laevigata	RLG00000005898 RLG00000005899 RLG00000005900
rosa_multiflora	Rmu_co8463221.1_g000001 Rmu_sc0001426.1_g000010 Rmu_sc0001426.1_g000011 Rmu_sc0001426.1_g000013 Rmu_sc0008739.1_g000001
rosa_roxburghii	Rroxscaffold_5G00384250 Rroxscaffold_5G00384260 Rroxscaffold_5G00384270
rosa_rugosa	Rorug05G0496400 Rorug05G0496500
rosa_samantha	Rh4AG406400 Rh4AG406500 Rh4AG406600 Rh4BG417400 Rh4BG417500 Rh4BG417600 Rh4BG417700 Rh4CG431900 Rh4DG413000
rosa_wichuraiana	Rw4G034900 Rw4G034910 Rw4G034920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	854, 1136
AasI	GACNNNNNNGTC	1 cut(s)	988
Acc65I	GGTACC	1 cut(s)	1045
AccB1I	GGYRCC	2 cut(s)	1045, 1101
AccB7I	CCANNNNNTGG	2 cut(s)	205, 1242
AccI	GTMKAC	1 cut(s)	1480
AccII	CGCG	4 cut(s)	461, 497, 1203, 1507
AclWI	GGATC	3 cut(s)	1259, 1380, 1393
AcoI	YGGCCR	3 cut(s)	91, 207, 1339
AcsI	RAATTY	4 cut(s)	472, 650, 835, 1292
AcuI	CTGAAG	1 cut(s)	939
AcyI	GRCGYC	1 cut(s)	24
AfaI	GTAC	2 cut(s)	403, 1047
AfiI	CCNNNNNNNGG	8 cut(s)	150, 184, 205, 439, 739, 1242, 1553, 1651
AflIII	ACRYGT	1 cut(s)	1505
AhlI	ACTAGT	1 cut(s)	847
AjnI	CCWGG	2 cut(s)	93, 198
AleI	CACNNNNGTG	1 cut(s)	1508
AluBI	AGCT	7 cut(s)	343, 540, 592, 1060, 1094, 1132, 1501
AluI	AGCT	7 cut(s)	343, 540, 592, 1060, 1094, 1132, 1501
Alw21I	GWGCWC	3 cut(s)	345, 1503, 1515
Alw26I	GTCTC	3 cut(s)	244, 314, 1008
AlwI	GGATC	3 cut(s)	1259, 1380, 1393
Ama87I	CYCGRG	1 cut(s)	543
AoxI	GGCC	7 cut(s)	91, 157, 207, 1275, 1339, 1359, 1460
ApeKI	GCWGC	1 cut(s)	1285
ApoI	RAATTY	4 cut(s)	472, 650, 835, 1292
ArsI	GACNNNNNNTTYG	4 cut(s)	9, 41, 1568, 1600
AsiSI	GCGATCGC	1 cut(s)	1201
Asp700I	GAANNNNTTC	1 cut(s)	759
Asp718I	GGTACC	1 cut(s)	1045
AspLEI	GCGC	2 cut(s)	917, 1381
AspS9I	GGNCC	6 cut(s)	158, 193, 430, 730, 1276, 1360
AsuC2I	CCSGG	4 cut(s)	544, 545, 1363, 1548
AsuHPI	GGTGA	5 cut(s)	166, 259, 448, 511, 1691
AsuII	TTCGAA	1 cut(s)	560
AvaI	CYCGRG	1 cut(s)	543
AvaII	GGWCC	3 cut(s)	193, 430, 730
BaeGI	GKGCMC	1 cut(s)	1106
BaeI	ACNNNNGTAYC	2 cut(s)	1037, 1070
BamHI	GGATCC	1 cut(s)	1385
BanI	GGYRCC	2 cut(s)	1045, 1101
BanII	GRGCYC	2 cut(s)	345, 1503
BauI	CACGAG	2 cut(s)	148, 1496
BbsI	GAAGAC	2 cut(s)	24, 878
Bbv12I	GWGCWC	3 cut(s)	345, 1503, 1515
BbvI	GCAGC	1 cut(s)	1297
BccI	CCATC	7 cut(s)	375, 404, 437, 560, 860, 1259, 1334
BceAI	ACGGC	8 cut(s)	25, 37, 103, 274, 769, 935, 1354, 1674
BciT130I	CCWGG	2 cut(s)	95, 200
BciVI	GTATCC	2 cut(s)	266, 595
BcnI	CCSGG	4 cut(s)	544, 545, 1363, 1548
BcoDI	GTCTC	3 cut(s)	244, 314, 1008
BcuI	ACTAGT	1 cut(s)	847
BfaI	CTAG	3 cut(s)	537, 848, 863
BfmI	CTRYAG	1 cut(s)	1283
BfoI	RGCGCY	1 cut(s)	918
BfuI	GTATCC	2 cut(s)	266, 595
BglI	GCCNNNNNGGC	1 cut(s)	1338
BisI	GCNGC	7 cut(s)	91, 157, 166, 210, 495, 891, 1286
BlsI	GCNGC	7 cut(s)	92, 158, 167, 211, 496, 892, 1287
Bme1390I	CCNGG	6 cut(s)	95, 200, 544, 545, 1363, 1548
Bme18I	GGWCC	3 cut(s)	193, 430, 730
BmeT110I	CYCGRG	1 cut(s)	543
BmgT120I	GGNCC	6 cut(s)	158, 193, 430, 730, 1276, 1360
BmiI	GGNNCC	5 cut(s)	1039, 1047, 1103, 1387, 1545
BmrFI	CCNGG	6 cut(s)	95, 200, 544, 545, 1363, 1548
BmsI	GCATC	1 cut(s)	373
BpiI	GAAGAC	2 cut(s)	24, 878
Bpu10I	CCTNAGC	1 cut(s)	1128
Bpu14I	TTCGAA	1 cut(s)	560
BpuEI	CTTGAG	4 cut(s)	320, 538, 851, 1177
BpuMI	CCSGG	4 cut(s)	544, 545, 1363, 1548
BsaBI	GATNNNNATC	2 cut(s)	447, 1203
BsaHI	GRCGYC	1 cut(s)	24
BsaI	GGTCTC	1 cut(s)	314
BsaWI	WCCGGW	2 cut(s)	116, 269
BsaXI	ACNNNNNCTCC	4 cut(s)	497, 527, 1421, 1451
Bsc4I	CCNNNNNNNGG	8 cut(s)	150, 184, 205, 439, 739, 1242, 1553, 1651
Bse118I	RCCGGY	1 cut(s)	168
Bse3DI	GCAATG	1 cut(s)	779
Bse8I	GATNNNNATC	2 cut(s)	447, 1203
BseBI	CCWGG	2 cut(s)	95, 200
BseGI	GGATG	4 cut(s)	448, 571, 1251, 1645
BseJI	GATNNNNATC	2 cut(s)	447, 1203
BseLI	CCNNNNNNNGG	8 cut(s)	150, 184, 205, 439, 739, 1242, 1553, 1651
BseMI	GCAATG	1 cut(s)	779
BseMII	CTCAG	7 cut(s)	90, 671, 951, 1047, 1104, 1363, 1455
BseRI	GAGGAG	2 cut(s)	43, 65
BseSI	GKGCMC	1 cut(s)	1106
BseXI	GCAGC	1 cut(s)	1297
BseYI	CCCAGC	2 cut(s)	1004, 1064
Bsh1236I	CGCG	4 cut(s)	461, 497, 1203, 1507
Bsh1285I	CGRYCG	1 cut(s)	1201
BshFI	GGCC	7 cut(s)	93, 159, 209, 1277, 1341, 1361, 1462
BshNI	GGYRCC	2 cut(s)	1045, 1101
BsiEI	CGRYCG	1 cut(s)	1201
BsiHKAI	GWGCWC	3 cut(s)	345, 1503, 1515
BsiHKCI	CYCGRG	1 cut(s)	543
BsiSI	CCGG	6 cut(s)	117, 169, 270, 544, 1363, 1547
BslFI	GGGAC	1 cut(s)	427
BslI	CCNNNNNNNGG	8 cut(s)	150, 184, 205, 439, 739, 1242, 1553, 1651
BsmAI	GTCTC	3 cut(s)	244, 314, 1008
BsmBI	CGTCTC	1 cut(s)	244
BsmFI	GGGAC	1 cut(s)	427
BsmI	GAATGC	2 cut(s)	1118, 1308
BsnI	GGCC	7 cut(s)	93, 159, 209, 1277, 1341, 1361, 1462
Bso31I	GGTCTC	1 cut(s)	314
BsoBI	CYCGRG	1 cut(s)	543
Bsp119I	TTCGAA	1 cut(s)	560
Bsp1286I	GDGCHC	4 cut(s)	345, 1106, 1503, 1515
Bsp143I	GATC	8 cut(s)	102, 826, 1152, 1198, 1204, 1264, 1385, 1588
Bsp68I	TCGCGA	1 cut(s)	1203
BspANI	GGCC	7 cut(s)	93, 159, 209, 1277, 1341, 1361, 1462
BspCNI	CTCAG	7 cut(s)	91, 670, 952, 1048, 1103, 1362, 1456
BspFNI	CGCG	4 cut(s)	461, 497, 1203, 1507
BspLI	GGNNCC	5 cut(s)	1039, 1047, 1103, 1387, 1545
BspMAI	CTGCAG	1 cut(s)	1287
BspPI	GGATC	3 cut(s)	1259, 1380, 1393
BspQI	GCTCTTC	2 cut(s)	350, 1404
BspT104I	TTCGAA	1 cut(s)	560
BspT107I	GGYRCC	2 cut(s)	1045, 1101
BspTNI	GGTCTC	1 cut(s)	314
BsrDI	GCAATG	1 cut(s)	779
BsrFI	RCCGGY	1 cut(s)	168
BssAI	RCCGGY	1 cut(s)	168
BssMI	GATC	8 cut(s)	102, 826, 1152, 1198, 1204, 1264, 1385, 1588
BssNAI	GTATAC	1 cut(s)	1481
BssNI	GRCGYC	1 cut(s)	24
BssSI	CACGAG	2 cut(s)	148, 1496
BssT1I	CCWWGG	5 cut(s)	1041, 1241, 1268, 1303, 1650
Bst1107I	GTATAC	1 cut(s)	1481
Bst2BI	CACGAG	2 cut(s)	148, 1496
Bst2UI	CCWGG	2 cut(s)	95, 200
Bst4CI	ACNGT	4 cut(s)	803, 990, 1037, 1679
Bst6I	CTCTTC	6 cut(s)	247, 350, 732, 900, 1404, 1469
BstACI	GRCGYC	1 cut(s)	24
BstAPI	GCANNNNNTGC	2 cut(s)	1078, 1578
BstBI	TTCGAA	1 cut(s)	560
BstC8I	GCNNGC	5 cut(s)	817, 957, 1062, 1355, 1359
BstDEI	CTNAG	8 cut(s)	99, 657, 960, 1056, 1090, 1128, 1349, 1464
BstDSI	CCRYGG	4 cut(s)	290, 433, 459, 495
BstENI	CCTNNNNNAGG	1 cut(s)	737
BstF5I	GGATG	4 cut(s)	448, 571, 1251, 1645
BstFNI	CGCG	4 cut(s)	461, 497, 1203, 1507
BstH2I	RGCGCY	1 cut(s)	918
BstHHI	GCGC	2 cut(s)	917, 1381
BstKTI	GATC	8 cut(s)	105, 829, 1155, 1201, 1207, 1267, 1388, 1591
BstMAI	GTCTC	3 cut(s)	244, 314, 1008
BstMBI	GATC	8 cut(s)	102, 826, 1152, 1198, 1204, 1264, 1385, 1588
BstMCI	CGRYCG	1 cut(s)	1201
BstMWI	GCNNNNNNNGC	9 cut(s)	162, 165, 956, 1078, 1322, 1338, 1363, 1420, 1578
BstNI	CCWGG	2 cut(s)	95, 200
BstSCI	CCNGG	6 cut(s)	93, 198, 542, 543, 1361, 1546
BstSFI	CTRYAG	1 cut(s)	1283
BstSLI	GKGCMC	1 cut(s)	1106
BstUI	CGCG	4 cut(s)	461, 497, 1203, 1507
BstV1I	GCAGC	1 cut(s)	1297
BstV2I	GAAGAC	2 cut(s)	24, 878
BstX2I	RGATCY	1 cut(s)	1385
BstYI	RGATCY	1 cut(s)	1385
BstZ17I	GTATAC	1 cut(s)	1481
BsuI	GTATCC	2 cut(s)	266, 595
BsuRI	GGCC	7 cut(s)	93, 159, 209, 1277, 1341, 1361, 1462
BtgI	CCRYGG	4 cut(s)	290, 433, 459, 495
BtsCI	GGATG	4 cut(s)	448, 571, 1251, 1645
BtuMI	TCGCGA	1 cut(s)	1203
Cac8I	GCNNGC	5 cut(s)	817, 957, 1062, 1355, 1359
CfoI	GCGC	2 cut(s)	917, 1381
Cfr10I	RCCGGY	1 cut(s)	168
Cfr13I	GGNCC	6 cut(s)	158, 193, 430, 730, 1276, 1360
Cfr42I	CCGCGG	2 cut(s)	462, 498
Cfr9I	CCCGGG	1 cut(s)	543
CseI	GACGC	2 cut(s)	13, 1400
Csp6I	GTAC	2 cut(s)	402, 1046
CspCI	CAANNNNNGTGG	2 cut(s)	168, 203
CviAII	CATG	9 cut(s)	642, 677, 771, 1051, 1110, 1123, 1237, 1382, 1487
CviQI	GTAC	2 cut(s)	402, 1046
DdeI	CTNAG	8 cut(s)	99, 657, 960, 1056, 1090, 1128, 1349, 1464
DpnI	GATC	8 cut(s)	104, 828, 1154, 1200, 1206, 1266, 1387, 1590
DpnII	GATC	8 cut(s)	102, 826, 1152, 1198, 1204, 1264, 1385, 1588
DrdI	GACNNNNNNGTC	1 cut(s)	988
DseDI	GACNNNNNNGTC	1 cut(s)	988
EaeI	YGGCCR	3 cut(s)	91, 207, 1339
Eam1104I	CTCTTC	6 cut(s)	247, 350, 732, 900, 1404, 1469
EarI	CTCTTC	6 cut(s)	247, 350, 732, 900, 1404, 1469
EciI	GGCGGA	3 cut(s)	46, 133, 455
Ecl136II	GAGCTC	2 cut(s)	343, 1501
Eco130I	CCWWGG	5 cut(s)	1041, 1241, 1268, 1303, 1650
Eco24I	GRGCYC	2 cut(s)	345, 1503
Eco31I	GGTCTC	1 cut(s)	314
Eco32I	GATATC	1 cut(s)	723
Eco47I	GGWCC	3 cut(s)	193, 430, 730
Eco53kI	GAGCTC	2 cut(s)	343, 1501
Eco57I	CTGAAG	1 cut(s)	939
Eco88I	CYCGRG	1 cut(s)	543
EcoICRI	GAGCTC	2 cut(s)	343, 1501
EcoNI	CCTNNNNNAGG	1 cut(s)	737
EcoO109I	RGGNCCY	1 cut(s)	1276
EcoRII	CCWGG	2 cut(s)	93, 198
EcoRV	GATATC	1 cut(s)	723
EcoT14I	CCWWGG	5 cut(s)	1041, 1241, 1268, 1303, 1650
EcoT38I	GRGCYC	2 cut(s)	345, 1503
ErhI	CCWWGG	5 cut(s)	1041, 1241, 1268, 1303, 1650
Esp3I	CGTCTC	1 cut(s)	244
FaeI	CATG	9 cut(s)	645, 680, 774, 1054, 1113, 1126, 1240, 1385, 1490
FalI	AAGNNNNNCTT	2 cut(s)	329, 361
FaqI	GGGAC	1 cut(s)	427
FatI	CATG	9 cut(s)	641, 676, 770, 1050, 1109, 1122, 1236, 1381, 1486
FblI	GTMKAC	1 cut(s)	1480
Fnu4HI	GCNGC	7 cut(s)	91, 157, 166, 210, 495, 891, 1286
FokI	GGATG	4 cut(s)	455, 578, 1238, 1652
FriOI	GRGCYC	2 cut(s)	345, 1503
Fsp4HI	GCNGC	7 cut(s)	91, 157, 166, 210, 495, 891, 1286
FspBI	CTAG	3 cut(s)	537, 848, 863
GlaI	GCGC	2 cut(s)	916, 1380
GluI	GCNGC	7 cut(s)	91, 157, 166, 210, 495, 891, 1286
GsaI	CCCAGC	2 cut(s)	1008, 1068
HaeII	RGCGCY	1 cut(s)	918
HaeIII	GGCC	7 cut(s)	93, 159, 209, 1277, 1341, 1361, 1462
HapII	CCGG	6 cut(s)	117, 169, 270, 544, 1363, 1547
HgaI	GACGC	2 cut(s)	13, 1400
HhaI	GCGC	2 cut(s)	917, 1381
Hin1I	GRCGYC	1 cut(s)	24
Hin1II	CATG	9 cut(s)	645, 680, 774, 1054, 1113, 1126, 1240, 1385, 1490
Hin6I	GCGC	2 cut(s)	915, 1379
HinP1I	GCGC	2 cut(s)	915, 1379
HindIII	AAGCTT	1 cut(s)	1130
HinfI	GANTC	4 cut(s)	260, 301, 361, 508
HpaII	CCGG	6 cut(s)	117, 169, 270, 544, 1363, 1547
HphI	GGTGA	5 cut(s)	166, 259, 448, 511, 1691
Hpy166II	GTNNAC	6 cut(s)	174, 196, 267, 456, 1404, 1481
Hpy188I	TCNGA	7 cut(s)	247, 313, 589, 1057, 1152, 1390, 1700
Hpy188III	TCNNGA	5 cut(s)	32, 280, 728, 830, 1202
Hpy8I	GTNNAC	6 cut(s)	174, 196, 267, 456, 1404, 1481
Hpy99I	CGWCG	2 cut(s)	17, 1394
HpyAV	CCTTC	8 cut(s)	481, 496, 556, 590, 682, 749, 1225, 1267
HpyCH4III	ACNGT	4 cut(s)	803, 990, 1037, 1679
HpyCH4V	TGCA	8 cut(s)	645, 1118, 1232, 1285, 1316, 1572, 1581, 1607
HpyF10VI	GCNNNNNNNGC	9 cut(s)	162, 165, 956, 1078, 1322, 1338, 1363, 1420, 1578
HpyF3I	CTNAG	8 cut(s)	99, 657, 960, 1056, 1090, 1128, 1349, 1464
Hsp92I	GRCGYC	1 cut(s)	24
Hsp92II	CATG	9 cut(s)	645, 680, 774, 1054, 1113, 1126, 1240, 1385, 1490
HspAI	GCGC	2 cut(s)	915, 1379
KpnI	GGTACC	1 cut(s)	1049
KspI	CCGCGG	2 cut(s)	462, 498
Kzo9I	GATC	8 cut(s)	102, 826, 1152, 1198, 1204, 1264, 1385, 1588
LguI	GCTCTTC	2 cut(s)	350, 1404
LmnI	GCTCC	4 cut(s)	340, 545, 1518, 1543
Lsp1109I	GCAGC	1 cut(s)	1297
LweI	GCATC	1 cut(s)	373
MaeI	CTAG	3 cut(s)	537, 848, 863
MaeIII	GTNAC	3 cut(s)	25, 990, 1673
MalI	GATC	8 cut(s)	104, 828, 1154, 1200, 1206, 1266, 1387, 1590
MboI	GATC	8 cut(s)	102, 826, 1152, 1198, 1204, 1264, 1385, 1588
MfeI	CAATTG	1 cut(s)	1424
MflI	RGATCY	1 cut(s)	1385
MhlI	GDGCHC	4 cut(s)	345, 1106, 1503, 1515
MluCI	AATT	7 cut(s)	420, 472, 650, 835, 855, 1292, 1424
MluI	ACGCGT	1 cut(s)	1505
MmeI	TCCRAC	2 cut(s)	1413, 1419
MroXI	GAANNNNTTC	1 cut(s)	759
MseI	TTAA	2 cut(s)	479, 789
MslI	CAYNNNNRTG	3 cut(s)	885, 1508, 1524
MspA1I	CMGCKG	2 cut(s)	461, 497
MspI	CCGG	6 cut(s)	117, 169, 270, 544, 1363, 1547
MspR9I	CCNGG	6 cut(s)	95, 200, 544, 545, 1363, 1548
MunI	CAATTG	1 cut(s)	1424
Mva1269I	GAATGC	2 cut(s)	1118, 1308
MvaI	CCWGG	2 cut(s)	95, 200
MvnI	CGCG	4 cut(s)	461, 497, 1203, 1507
MwoI	GCNNNNNNNGC	9 cut(s)	162, 165, 956, 1078, 1322, 1338, 1363, 1420, 1578
NciI	CCSGG	4 cut(s)	544, 545, 1363, 1548
NdeII	GATC	8 cut(s)	102, 826, 1152, 1198, 1204, 1264, 1385, 1588
NlaIII	CATG	9 cut(s)	645, 680, 774, 1054, 1113, 1126, 1240, 1385, 1490
NlaIV	GGNNCC	5 cut(s)	1039, 1047, 1103, 1387, 1545
NmuCI	GTSAC	3 cut(s)	25, 990, 1673
NruI	TCGCGA	1 cut(s)	1203
NspV	TTCGAA	1 cut(s)	560
OliI	CACNNNNGTG	1 cut(s)	1508
PciSI	GCTCTTC	2 cut(s)	350, 1404
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 287
PctI	GAATGC	2 cut(s)	1118, 1308
PdmI	GAANNNNTTC	1 cut(s)	759
PfeI	GAWTC	4 cut(s)	260, 301, 361, 508
PflFI	GACNNNGTC	1 cut(s)	23
PflMI	CCANNNNNTGG	2 cut(s)	205, 1242
PkrI	GCNGC	7 cut(s)	92, 158, 167, 211, 496, 892, 1287
Ple19I	CGATCG	1 cut(s)	1201
PsiI	TTATAA	2 cut(s)	854, 1136
Psp124BI	GAGCTC	2 cut(s)	345, 1503
Psp6I	CCWGG	2 cut(s)	93, 198
PspFI	CCCAGC	2 cut(s)	1004, 1064
PspGI	CCWGG	2 cut(s)	93, 198
PspN4I	GGNNCC	5 cut(s)	1039, 1047, 1103, 1387, 1545
PspPI	GGNCC	6 cut(s)	158, 193, 430, 730, 1276, 1360
PsrI	GAACNNNNNNTAC	2 cut(s)	969, 1001
PstI	CTGCAG	1 cut(s)	1287
PsuI	RGATCY	1 cut(s)	1385
PsyI	GACNNNGTC	1 cut(s)	23
PvuI	CGATCG	1 cut(s)	1201
RgaI	GCGATCGC	1 cut(s)	1201
RruI	TCGCGA	1 cut(s)	1203
RsaI	GTAC	2 cut(s)	403, 1047
RsaNI	GTAC	2 cut(s)	402, 1046
RseI	CAYNNNNRTG	3 cut(s)	885, 1508, 1524
SacI	GAGCTC	2 cut(s)	345, 1503
SacII	CCGCGG	2 cut(s)	462, 498
SapI	GCTCTTC	2 cut(s)	350, 1404
SaqAI	TTAA	2 cut(s)	479, 789
SatI	GCNGC	7 cut(s)	91, 157, 166, 210, 495, 891, 1286
Sau3AI	GATC	8 cut(s)	102, 826, 1152, 1198, 1204, 1264, 1385, 1588
Sau96I	GGNCC	6 cut(s)	158, 193, 430, 730, 1276, 1360
ScrFI	CCNGG	6 cut(s)	95, 200, 544, 545, 1363, 1548
SduI	GDGCHC	4 cut(s)	345, 1106, 1503, 1515
SfaAI	GCGATCGC	1 cut(s)	1201
SfaNI	GCATC	1 cut(s)	373
SfcI	CTRYAG	1 cut(s)	1283
Sfr303I	CCGCGG	2 cut(s)	462, 498
SfuI	TTCGAA	1 cut(s)	560
SgfI	GCGATCGC	1 cut(s)	1201
SgrBI	CCGCGG	2 cut(s)	462, 498
SinI	GGWCC	3 cut(s)	193, 430, 730
SmaI	CCCGGG	1 cut(s)	545
SmiMI	CAYNNNNRTG	3 cut(s)	885, 1508, 1524
SmlI	CTYRAG	4 cut(s)	335, 517, 830, 1192
SmoI	CTYRAG	4 cut(s)	335, 517, 830, 1192
SpeI	ACTAGT	1 cut(s)	847
Sse9I	AATT	7 cut(s)	420, 472, 650, 835, 855, 1292, 1424
SspMI	CTAG	3 cut(s)	537, 848, 863
SstI	GAGCTC	2 cut(s)	345, 1503
StyD4I	CCNGG	6 cut(s)	93, 198, 542, 543, 1361, 1546
StyI	CCWWGG	5 cut(s)	1041, 1241, 1268, 1303, 1650
TaaI	ACNGT	4 cut(s)	803, 990, 1037, 1679
TaqI	TCGA	5 cut(s)	281, 299, 560, 972, 1587
TasI	AATT	7 cut(s)	420, 472, 650, 835, 855, 1292, 1424
TatI	WGTACW	1 cut(s)	401
TauI	GCSGC	6 cut(s)	93, 159, 168, 212, 497, 893
TfiI	GAWTC	4 cut(s)	260, 301, 361, 508
Tru1I	TTAA	2 cut(s)	479, 789
Tru9I	TTAA	2 cut(s)	479, 789
TseFI	GTSAC	3 cut(s)	25, 990, 1673
TseI	GCWGC	1 cut(s)	1285
Tsp45I	GTSAC	3 cut(s)	25, 990, 1673
TspDTI	ATGAA	3 cut(s)	485, 701, 1111
TspGWI	ACGGA	4 cut(s)	441, 1389, 1649, 1673
TspMI	CCCGGG	1 cut(s)	543
Tth111I	GACNNNGTC	1 cut(s)	23
Van91I	CCANNNNNTGG	2 cut(s)	205, 1242
VpaK11BI	GGWCC	3 cut(s)	193, 430, 730
XagI	CCTNNNNNAGG	1 cut(s)	737
XapI	RAATTY	4 cut(s)	472, 650, 835, 1292
XcmI	CCANNNNNNNNNTGG	1 cut(s)	440
XmaI	CCCGGG	1 cut(s)	543
XmiI	GTMKAC	1 cut(s)	1480
XmnI	GAANNNNTTC	1 cut(s)	759
XspI	CTAG	3 cut(s)	537, 848, 863

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.