Rw4G034900

Domain of unknown function DUF220

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001775
Physical Location & Seq
Reverse (-)
61823105 .. 61825373
2269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034900.1

Sequence Viewer

Length: 999 bp
ATGGGTGAGACAGAGGTGCCCACGACAAGCATAGACATCCGGAAGAACGAGAAAAATGGGCGCCTCAAGGCATTGGATGTAGTGCTCTCTAAAGCTTTTGTCCAGGTTCCTCACAAGCTACAGAATTTTCTTAAGTCACAGTTTAGAAGACTACCAAAAGCTGATGGAGTTAAAACAGTGAGCTCTGTCCTTAAAAAGGCTTCATCTTTGGAGGTTGACCTGGAAAAGCAGTTGCAAGCATGGAGAGAAAATCCTTCATGGGATGATCAAACACCACATATAAAGGTCAGTGTGCCAAAAGGCTCTCTCTGCAACCTCAGTGTAAACGTCAATGTTGGGTTGCCCCCAGATGCAGTATATAATATTGTAACTGACCCCGACAATAAGAGGGTTTTCAAGAATATTAAGGAGGTGATATCCAGAAGAGTTTTGGTTGACGAAGGTCTTAGGCAGGTTGTTGATCTAGAGCAAGCAGCTATATGGAAATTTCTTTGGTGGTCAGGGACCATTGCAGTTCATGTTTTAGTAGATCAAAACAGAGAAGACCACTCGATGAAGTTCAAGCAAGTCAAAACAGGGTTTATGGAGAGATTTGAAGGCTGCTGGAGAGTGGAGCCTCTTTTTGTTGATGAAAAAACCTGCTTTCCCTATAAACCCACAACATGGGCAGACTATAATTCACACACTGGAGGCAAAGGAAGGATCGGATCAAAGGTGAGCTTGGACCAACTGATCCAACCGTCTATTGTTCCTCCCCCACCCATTTCTTGGTATCTGAGGGGTATCACCGCCAAGACCACAGAGATGTTGATAAATGATCTTCTTGCTGAAGCCGCCAGAATACGGGGAGAATTAAACCCTGAGAAGTCTGATAAAGAGGTTGAAATGTCTGAGGAAGTACTTAATCAAGTTGATAACACCTGTAACATCAAAGAAAGATGGGCTATGCATAGGAGAAATGCAAAGCAATGTCATCGGAGGCTACCAACTGCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF220

Protein Analysis

332

Amino Acids

37.92

Weight (kDa)

9.22

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF220 PF02713 167 - 238 4.8e-29 Domain of unknown function DUF220
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 442, 647
AccB1I GGYRCC 2 cut(s) 16, 60
AccB7I CCANNNNNTGG 2 cut(s) 663, 768
AccIII TCCGGA 1 cut(s) 39
AciI CCGC 2 cut(s) 789, 834
AclWI GGATC 3 cut(s) 710, 715, 727
AcsI RAATTY 2 cut(s) 124, 485
AcuI CTGAAG 1 cut(s) 849
AcyI GRCGYC 1 cut(s) 61
AfaI GTAC 1 cut(s) 900
AfiI CCNNNNNNNGG 4 cut(s) 196, 663, 768, 843
AflII CTTAAG 1 cut(s) 131
AgsI TTSAA 4 cut(s) 397, 562, 596, 884
AjnI CCWGG 2 cut(s) 102, 219
AluBI AGCT 6 cut(s) 95, 118, 161, 183, 476, 720
AluI AGCT 6 cut(s) 95, 118, 161, 183, 476, 720
Alw21I GWGCWC 2 cut(s) 87, 185
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 3 cut(s) 710, 715, 727
Aor13HI TCCGGA 1 cut(s) 39
ApeKI GCWGC 2 cut(s) 473, 600
ApoI RAATTY 2 cut(s) 124, 485
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 2 cut(s) 504, 724
AsuHPI GGTGA 4 cut(s) 17, 424, 727, 778
AvaII GGWCC 2 cut(s) 504, 724
BaeGI GKGCMC 1 cut(s) 21
BanI GGYRCC 2 cut(s) 16, 60
BanII GRGCYC 1 cut(s) 185
BbsI GAAGAC 2 cut(s) 154, 549
Bbv12I GWGCWC 2 cut(s) 87, 185
BbvI GCAGC 2 cut(s) 485, 587
BccI CCATC 2 cut(s) 158, 933
BcgI CGANNNNNNTGC 2 cut(s) 956, 990
BciT130I CCWGG 2 cut(s) 104, 221
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 1 cut(s) 2
BfaI CTAG 1 cut(s) 464
BfmI CTRYAG 1 cut(s) 119
BfoI RGCGCY 1 cut(s) 64
BfrI CTTAAG 1 cut(s) 131
BfuAI ACCTGC 2 cut(s) 442, 647
BisI GCNGC 3 cut(s) 474, 601, 834
BlsI GCNGC 3 cut(s) 475, 602, 835
BmcAI AGTACT 1 cut(s) 900
Bme1390I CCNGG 2 cut(s) 104, 221
Bme18I GGWCC 2 cut(s) 504, 724
BmgT120I GGNCC 2 cut(s) 504, 724
BmiI GGNNCC 5 cut(s) 18, 62, 108, 505, 615
BmrFI CCNGG 2 cut(s) 104, 221
BmsI GCATC 1 cut(s) 340
BoxI GACNNNNGTC 1 cut(s) 441
BpiI GAAGAC 2 cut(s) 154, 549
BpmI CTGGAG 2 cut(s) 625, 708
BpuEI CTTGAG 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 61
BsaWI WCCGGW 1 cut(s) 39
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 663, 768, 843
Bse1I ACTGG 1 cut(s) 691
Bse3DI GCAATG 2 cut(s) 507, 974
BseAI TCCGGA 1 cut(s) 39
BseBI CCWGG 2 cut(s) 104, 221
BseGI GGATG 3 cut(s) 36, 82, 268
BseLI CCNNNNNNNGG 4 cut(s) 196, 663, 768, 843
BseMI GCAATG 2 cut(s) 507, 974
BseMII CTCAG 4 cut(s) 331, 767, 852, 882
BseNI ACTGG 1 cut(s) 691
BseSI GKGCMC 1 cut(s) 21
BseXI GCAGC 2 cut(s) 485, 587
BshNI GGYRCC 2 cut(s) 16, 60
BsiHKAI GWGCWC 2 cut(s) 87, 185
BsiSI CCGG 1 cut(s) 40
BslFI GGGAC 1 cut(s) 517
BslI CCNNNNNNNGG 4 cut(s) 196, 663, 768, 843
BsmAI GTCTC 1 cut(s) 2
BsmFI GGGAC 1 cut(s) 517
Bsp1286I GDGCHC 3 cut(s) 21, 87, 185
Bsp13I TCCGGA 1 cut(s) 39
Bsp143I GATC 7 cut(s) 265, 460, 529, 702, 707, 732, 817
BspACI CCGC 2 cut(s) 789, 834
BspCNI CTCAG 4 cut(s) 330, 768, 853, 883
BspEI TCCGGA 1 cut(s) 39
BspLI GGNNCC 5 cut(s) 18, 62, 108, 505, 615
BspMI ACCTGC 2 cut(s) 442, 647
BspPI GGATC 3 cut(s) 710, 715, 727
BspT107I GGYRCC 2 cut(s) 16, 60
BspTI CTTAAG 1 cut(s) 131
BsrDI GCAATG 2 cut(s) 507, 974
BsrI ACTGG 1 cut(s) 691
BssMI GATC 7 cut(s) 265, 460, 529, 702, 707, 732, 817
BssNI GRCGYC 1 cut(s) 61
Bst2UI CCWGG 2 cut(s) 104, 221
Bst4CI ACNGT 3 cut(s) 141, 178, 741
Bst6I CTCTTC 1 cut(s) 418
BstACI GRCGYC 1 cut(s) 61
BstAFI CTTAAG 1 cut(s) 131
BstC8I GCNNGC 2 cut(s) 237, 471
BstDEI CTNAG 5 cut(s) 317, 446, 776, 861, 891
BstENI CCTNNNNNAGG 1 cut(s) 194
BstF5I GGATG 3 cut(s) 36, 82, 268
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 7 cut(s) 268, 463, 532, 705, 710, 735, 820
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 7 cut(s) 265, 460, 529, 702, 707, 732, 817
BstMWI GCNNNNNNNGC 2 cut(s) 309, 833
BstNI CCWGG 2 cut(s) 104, 221
BstPAI GACNNNNGTC 1 cut(s) 441
BstSCI CCNGG 2 cut(s) 102, 219
BstSFI CTRYAG 1 cut(s) 119
BstSLI GKGCMC 1 cut(s) 21
BstV1I GCAGC 2 cut(s) 485, 587
BstV2I GAAGAC 2 cut(s) 154, 549
BtsCI GGATG 3 cut(s) 36, 82, 268
BtsIMutI CAGTG 4 cut(s) 183, 295, 325, 684
BveI ACCTGC 2 cut(s) 442, 647
Cac8I GCNNGC 2 cut(s) 237, 471
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 2 cut(s) 504, 724
Csp6I GTAC 1 cut(s) 899
CviAII CATG 4 cut(s) 240, 258, 518, 663
CviQI GTAC 1 cut(s) 899
DdeI CTNAG 5 cut(s) 317, 446, 776, 861, 891
DinI GGCGCC 1 cut(s) 62
DpnI GATC 7 cut(s) 267, 462, 531, 704, 709, 734, 819
DpnII GATC 7 cut(s) 265, 460, 529, 702, 707, 732, 817
Eam1104I CTCTTC 1 cut(s) 418
EarI CTCTTC 1 cut(s) 418
Ecl136II GAGCTC 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 185
Eco32I GATATC 1 cut(s) 417
Eco47I GGWCC 2 cut(s) 504, 724
Eco53kI GAGCTC 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 849
EcoICRI GAGCTC 1 cut(s) 183
EcoNI CCTNNNNNAGG 1 cut(s) 194
EcoRII CCWGG 2 cut(s) 102, 219
EcoRV GATATC 1 cut(s) 417
EcoT22I ATGCAT 1 cut(s) 951
EcoT38I GRGCYC 1 cut(s) 185
EgeI GGCGCC 1 cut(s) 62
EheI GGCGCC 1 cut(s) 62
FaeI CATG 4 cut(s) 243, 261, 521, 666
FalI AAGNNNNNCTT 2 cut(s) 704, 736
FaqI GGGAC 1 cut(s) 517
FatI CATG 4 cut(s) 239, 257, 517, 662
FbaI TGATCA 1 cut(s) 265
Fnu4HI GCNGC 3 cut(s) 474, 601, 834
FokI GGATG 3 cut(s) 23, 89, 275
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 3 cut(s) 474, 601, 834
FspBI CTAG 1 cut(s) 464
GlaI GCGC 1 cut(s) 62
GluI GCNGC 3 cut(s) 474, 601, 834
GsuI CTGGAG 2 cut(s) 625, 708
HaeII RGCGCY 1 cut(s) 64
HapII CCGG 1 cut(s) 40
HhaI GCGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 61
Hin1II CATG 4 cut(s) 243, 261, 521, 666
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 2 cut(s) 217, 436
HindII GTYRAC 2 cut(s) 217, 436
HindIII AAGCTT 1 cut(s) 93
HpaII CCGG 1 cut(s) 40
HphI GGTGA 4 cut(s) 17, 424, 727, 778
Hpy166II GTNNAC 3 cut(s) 217, 325, 436
Hpy188I TCNGA 5 cut(s) 707, 777, 871, 892, 978
Hpy188III TCNNGA 4 cut(s) 40, 397, 420, 464
Hpy8I GTNNAC 3 cut(s) 217, 325, 436
HpyAV CCTTC 4 cut(s) 264, 434, 590, 693
HpyCH4III ACNGT 3 cut(s) 141, 178, 741
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 6 cut(s) 235, 312, 353, 512, 949, 962
HpyF10VI GCNNNNNNNGC 2 cut(s) 309, 833
HpyF3I CTNAG 5 cut(s) 317, 446, 776, 861, 891
HpySE526I ACGT 1 cut(s) 327
Hsp92I GRCGYC 1 cut(s) 61
Hsp92II CATG 4 cut(s) 243, 261, 521, 666
HspAI GCGC 1 cut(s) 61
KasI GGCGCC 1 cut(s) 60
Kpn2I TCCGGA 1 cut(s) 39
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 7 cut(s) 265, 460, 529, 702, 707, 732, 817
LmnI GCTCC 1 cut(s) 613
Lsp1109I GCAGC 2 cut(s) 485, 587
LweI GCATC 1 cut(s) 340
MaeI CTAG 1 cut(s) 464
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 3 cut(s) 135, 367, 923
MalI GATC 7 cut(s) 267, 462, 531, 704, 709, 734, 819
MboI GATC 7 cut(s) 265, 460, 529, 702, 707, 732, 817
MboII GAAGA 5 cut(s) 55, 159, 435, 554, 812
MhlI GDGCHC 3 cut(s) 21, 87, 185
MluCI AATT 4 cut(s) 124, 485, 676, 851
Mly113I GGCGCC 1 cut(s) 61
MmeI TCCRAC 1 cut(s) 760
Mph1103I ATGCAT 1 cut(s) 951
MroI TCCGGA 1 cut(s) 39
MseI TTAA 6 cut(s) 132, 171, 192, 405, 854, 903
MslI CAYNNNNRTG 1 cut(s) 803
MspCI CTTAAG 1 cut(s) 131
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 2 cut(s) 104, 221
MvaI CCWGG 2 cut(s) 104, 221
MwoI GCNNNNNNNGC 2 cut(s) 309, 833
NarI GGCGCC 1 cut(s) 61
NdeII GATC 7 cut(s) 265, 460, 529, 702, 707, 732, 817
NlaIII CATG 4 cut(s) 243, 261, 521, 666
NlaIV GGNNCC 5 cut(s) 18, 62, 108, 505, 615
NmuCI GTSAC 1 cut(s) 135
NsiI ATGCAT 1 cut(s) 951
PflMI CCANNNNNTGG 2 cut(s) 663, 768
PkrI GCNGC 3 cut(s) 475, 602, 835
PluTI GGCGCC 1 cut(s) 64
PshAI GACNNNNGTC 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 185
Psp6I CCWGG 2 cut(s) 102, 219
PspGI CCWGG 2 cut(s) 102, 219
PspN4I GGNNCC 5 cut(s) 18, 62, 108, 505, 615
PspPI GGNCC 2 cut(s) 504, 724
RsaI GTAC 1 cut(s) 900
RsaNI GTAC 1 cut(s) 899
RseI CAYNNNNRTG 1 cut(s) 803
SacI GAGCTC 1 cut(s) 185
SaqAI TTAA 6 cut(s) 132, 171, 192, 405, 854, 903
SatI GCNGC 3 cut(s) 474, 601, 834
Sau3AI GATC 7 cut(s) 265, 460, 529, 702, 707, 732, 817
Sau96I GGNCC 2 cut(s) 504, 724
ScaI AGTACT 1 cut(s) 900
ScrFI CCNGG 2 cut(s) 104, 221
SduI GDGCHC 3 cut(s) 21, 87, 185
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 119
SfoI GGCGCC 1 cut(s) 62
SinI GGWCC 2 cut(s) 504, 724
SmiMI CAYNNNNRTG 1 cut(s) 803
SmlI CTYRAG 2 cut(s) 65, 131
SmoI CTYRAG 2 cut(s) 65, 131
Sse9I AATT 4 cut(s) 124, 485, 676, 851
SsiI CCGC 2 cut(s) 789, 834
SspDI GGCGCC 1 cut(s) 60
SspI AATATT 2 cut(s) 364, 403
SspMI CTAG 1 cut(s) 464
SstI GAGCTC 1 cut(s) 185
StyD4I CCNGG 2 cut(s) 102, 219
TaaI ACNGT 3 cut(s) 141, 178, 741
TaiI ACGT 1 cut(s) 330
TaqI TCGA 1 cut(s) 551
TasI AATT 4 cut(s) 124, 485, 676, 851
TatI WGTACW 1 cut(s) 898
TauI GCSGC 1 cut(s) 836
Tru1I TTAA 6 cut(s) 132, 171, 192, 405, 854, 903
Tru9I TTAA 6 cut(s) 132, 171, 192, 405, 854, 903
TscAI CASTG 4 cut(s) 183, 295, 325, 691
TseFI GTSAC 1 cut(s) 135
TseI GCWGC 2 cut(s) 473, 600
Tsp45I GTSAC 1 cut(s) 135
TspDTI ATGAA 5 cut(s) 192, 246, 506, 569, 645
TspRI CASTG 4 cut(s) 183, 295, 325, 691
Van91I CCANNNNNTGG 2 cut(s) 663, 768
Vha464I CTTAAG 1 cut(s) 131
VpaK11BI GGWCC 2 cut(s) 504, 724
XagI CCTNNNNNAGG 1 cut(s) 194
XapI RAATTY 2 cut(s) 124, 485
XbaI TCTAGA 1 cut(s) 463
XcmI CCANNNNNNNNNTGG 2 cut(s) 427, 765
XspI CTAG 1 cut(s) 464
ZrmI AGTACT 1 cut(s) 900
Zsp2I ATGCAT 1 cut(s) 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.