Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001775

Physical Location & Seq

Genomic Coordinates Forward (+)

6550912 .. 6553504

Gene Structure 2593 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1094600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1716 bp

ATGGCTAACGGCGACGGACACGGCGTCACTCACGACCTCCTTTCTCTCCTCGCCTCCGCCGACCGAAACTTCCTCCTCCGCAACAACGGCGACCAGGTTGAGATCAGTAGTTTGACCGGAAAGATTGTGGGGTTGTATTTCTCCGCCTCGTGGTGCGGCCCCTGCCGCCGGTTCACCCCAAACTTGGTGGAGGTTCACCAGGACTTGGCTGCCAAAGGCGACTTTGAAGTTGTGTTCATCTCTTCTGATAGAGATGAGGAATCGTTCAATGGATACTTCTCTAAAATGCCTTGGCTTTCGATTCCCTTTTCGGATTTGGAGACCCGGAAACGCCTCAAGGAGTTCTTCAAAGTCAGGGGGATTCCCCATCTTGTCATTATTGATGCCAATGGGAAGGTTTCTATTGATGATGGAACTAAAATTGTGATGGACCACGGCGTGGATGGTTATCCGTTTACTGCGGAGAAGATGAATTTCTTGATTGAGCAGGAAGCCGTGGCGAAGGAGAATCAGTCCTTGAGTTCTCTTTTGGTCTCTAACTCCCGGGATTATTTGATTTCGAAGGATGGGAATAAGGTTTCTGTGTCCGAGCTTGAAGGAAAGATTGTTGGATTGTATTTCTCGCTTTATACTCACAAGCCATGCAAAGAATTTACTCAGGCACTTGTGAAGTTCCATGAGGAACTGAAGGAGAAAGGAGAGAACTTTGAGATTGTATTGATATCTCTAGACCTTGAAGAGGAACACTTCAAAGAAGGATTTCAAGTGTCATGGTTGGCATTGCCGTTTAAGGACAAGAACTGTGAAAAACTTGCTCGCTACTTTGCGCTTGAGAGCTTACCTAGACTAGTTATAATTGGGCAAGATGGGAAGACCTTACACTCCAATGCGGCTGAACTCATTGAAGAGTATGGCGCTGAAGCCTATCCGTTTACAGCAGAGAAGATTGCTGAGCTTGCTGAATTGAAACTCGAAGAACAGACTGTACAGTCACTTTTGGTTGCTGGGGGCAGAGACTTTGTGGTTGAAAAGAACGGTTCCAGGGTACCCGTGTCTGAGCTTGCTGGGAAGCACATTCTGCTCTATTTCTCAGCTCATTGGTGCCGTCCATGTCGTGCATTCATGCCTAAGCTTATAAAAGCATACAATCAGATCAAGGCAAAGGACAATGCAATCGAAATAATCTTCCTCTCAAGCGATCGTGATCATCATTCCTTCAAAGAATTCTTTGAGACCATGCCTTGGTTAGCCATCCCATTAGGCGATCCAAGGAAGGCCCTCCTGCAGCGCAAATTCAAAATCCAAGCAATTCCTGCATTGGTAGCCATTAGCCCCAGCGGTCAGACACTCAGCACGCAGGCCCGGCAGCTCATACAGGCACATGGAGCCGATGCTTATCCTTTCACCGAAGAGCATTTGAAGCAATTGGAGGAAAAGCTGGAGGAAGAAGCAAAGGGGTGGCCTGAGAAAGTGAAGAGTATACTCCATGCTGAACACGAGCTCATTCGTGTGCTCCACCACGACTATGTTTGCTGGTGTAGGGAGCCGGGAAGTGGCTGGTCCTTTTACTGCAAAGACTGCGACTTCGATCTCCACCCAAGATGTGCTTTGGGCAACAATGGAGGAACAAAGGATGACCCTCCCACGGCGAAGGATGACCCTTCCAAGGCAGCCGAGGATTATATTTGTGACGGCGATGTGTGCCGTAAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0003824 GO:0004791 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006935 GO:0008150 GO:0008152 GO:0009605 GO:0009636 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010035 GO:0010038 GO:0010183 GO:0010769 GO:0015036 GO:0016043 GO:0016049 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0022414 GO:0022603 GO:0022604 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0040008 GO:0040011 GO:0042221 GO:0042330 GO:0043900 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0045595 GO:0046686 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050918 GO:0051128 GO:0051239 GO:0051510 GO:0051704 GO:0051716 GO:0055114 GO:0060284 GO:0060560 GO:0065007 GO:0070887 GO:0071840 GO:0080092 GO:0097237 GO:0098754 GO:0098869 GO:1990748 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C1_2 Thioredoxin_8

Protein Analysis

572

Amino Acids

64.39

Weight (kDa)

5.57

Isoelectric Point (pI)

29.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Redoxin	PF08534	26 - 134	7.9e-09	Redoxin
AhpC-TSA	PF00578	27 - 133	9.2e-09	AhpC/TSA family
Thioredoxin_2	PF13098	39 - 130	2.5e-07	Thioredoxin-like domain
Thioredoxin_8	PF13905	40 - 133	1.4e-29	Thioredoxin-like
Thioredoxin	PF00085	42 - 87	9.4e-06	Thioredoxin
Thioredoxin_8	PF13905	200 - 291	4.1e-22	Thioredoxin-like
Redoxin	PF08534	342 - 444	8.8e-07	Redoxin
AhpC-TSA	PF00578	344 - 447	2.9e-09	AhpC/TSA family
Thioredoxin_8	PF13905	356 - 448	2.3e-26	Thioredoxin-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70480 AT1G70480
fragaria_vesca	FvH4_4g34241 FvH4_4g34250 FvH4_4g34260 FvH4_4g34260 FvH4_4g34260 FvH4_4g34260
malus_domestica	MD13G1093300.v1.1 MD13G1093500.v1.1 MD16G1094500.v1.1 MD16G1094600.v1.1 MD16G1094700.v1.1
prunus_persica	Prupe.1G251900_v2.0.a1 Prupe.1G252000_v2.0.a1
pyrus_communis	pycom13g08130 pycom13g08140 pycom16g08030 pycom16g08040 pycom16g08050
rosa_chinensis	RchiOBHm_Chr4g0443711 RchiOBHm_Chr6g0250421
rosa_laevigata	RLG00000005898 RLG00000005899 RLG00000005900
rosa_multiflora	Rmu_co8463221.1_g000001 Rmu_sc0001426.1_g000010 Rmu_sc0001426.1_g000011 Rmu_sc0001426.1_g000013 Rmu_sc0008739.1_g000001
rosa_roxburghii	Rroxscaffold_5G00384250 Rroxscaffold_5G00384260 Rroxscaffold_5G00384270
rosa_rugosa	Rorug05G0496400 Rorug05G0496500
rosa_samantha	Rh4AG406400 Rh4AG406500 Rh4AG406600 Rh4BG417400 Rh4BG417500 Rh4BG417600 Rh4BG417700 Rh4CG431900 Rh4DG413000
rosa_wichuraiana	Rw4G034900 Rw4G034910 Rw4G034920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	854, 1136
AasI	GACNNNNNNGTC	1 cut(s)	988
Acc65I	GGTACC	1 cut(s)	1045
AccB1I	GGYRCC	2 cut(s)	1045, 1101
AccB7I	CCANNNNNTGG	3 cut(s)	205, 439, 1242
AccI	GTMKAC	1 cut(s)	1480
AciI	CCGC	8 cut(s)	57, 79, 144, 156, 166, 461, 890, 1338
AclWI	GGATC	1 cut(s)	1259
AcsI	RAATTY	4 cut(s)	472, 650, 1223, 1292
AcuI	CTGAAG	2 cut(s)	707, 939
AcyI	GRCGYC	1 cut(s)	24
AdeI	CACNNNGTG	1 cut(s)	439
AfaI	GTAC	2 cut(s)	987, 1047
AhdI	GACNNNNNGTC	1 cut(s)	23
AhlI	ACTAGT	1 cut(s)	847
AjnI	CCWGG	3 cut(s)	93, 198, 1040
AluBI	AGCT	9 cut(s)	592, 837, 955, 1060, 1094, 1132, 1369, 1438, 1501
AluI	AGCT	9 cut(s)	592, 837, 955, 1060, 1094, 1132, 1369, 1438, 1501
Alw21I	GWGCWC	2 cut(s)	1503, 1515
Alw26I	GTCTC	4 cut(s)	314, 538, 1008, 1226
AlwI	GGATC	1 cut(s)	1259
Ama87I	CYCGRG	1 cut(s)	543
AoxI	GGCC	4 cut(s)	157, 1275, 1359, 1460
ApeKI	GCWGC	4 cut(s)	209, 1285, 1366, 1670
ApoI	RAATTY	4 cut(s)	472, 650, 1223, 1292
ArsI	GACNNNNNNTTYG	2 cut(s)	1568, 1600
Asp700I	GAANNNNTTC	1 cut(s)	759
Asp718I	GGTACC	1 cut(s)	1045
AspLEI	GCGC	3 cut(s)	829, 917, 1290
AspS9I	GGNCC	5 cut(s)	158, 430, 1276, 1360, 1560
AsuC2I	CCSGG	5 cut(s)	325, 544, 545, 1363, 1548
AsuHPI	GGTGA	3 cut(s)	166, 188, 1396
AsuII	TTCGAA	1 cut(s)	560
AvaI	CYCGRG	1 cut(s)	543
AvaII	GGWCC	2 cut(s)	430, 1560
BaeI	ACNNNNGTAYC	2 cut(s)	1037, 1070
BanI	GGYRCC	2 cut(s)	1045, 1101
BanII	GRGCYC	1 cut(s)	1503
BauI	CACGAG	2 cut(s)	148, 1496
BbsI	GAAGAC	1 cut(s)	878
Bbv12I	GWGCWC	2 cut(s)	1503, 1515
BbvI	GCAGC	4 cut(s)	196, 1297, 1378, 1682
BccI	CCATC	7 cut(s)	375, 404, 421, 437, 560, 860, 1259
BciT130I	CCWGG	3 cut(s)	95, 200, 1042
BciVI	GTATCC	1 cut(s)	266
BclI	TGATCA	1 cut(s)	1204
BcnI	CCSGG	5 cut(s)	325, 544, 545, 1363, 1548
BcoDI	GTCTC	4 cut(s)	314, 538, 1008, 1226
BcuI	ACTAGT	1 cut(s)	847
BfaI	CTAG	3 cut(s)	728, 843, 848
BfmI	CTRYAG	1 cut(s)	1283
BfoI	RGCGCY	1 cut(s)	918
BfuI	GTATCC	1 cut(s)	266
BisI	GCNGC	7 cut(s)	157, 166, 210, 891, 1286, 1367, 1671
BlpI	GCTNAGC	1 cut(s)	951
BlsI	GCNGC	7 cut(s)	158, 167, 211, 892, 1287, 1368, 1672
Bme1390I	CCNGG	8 cut(s)	95, 200, 325, 544, 545, 1042, 1363, 1548
Bme18I	GGWCC	2 cut(s)	430, 1560
BmeRI	GACNNNNNGTC	1 cut(s)	23
BmeT110I	CYCGRG	1 cut(s)	543
BmgT120I	GGNCC	5 cut(s)	158, 430, 1276, 1360, 1560
BmiI	GGNNCC	6 cut(s)	160, 1039, 1047, 1103, 1387, 1545
BmrFI	CCNGG	8 cut(s)	95, 200, 325, 544, 545, 1042, 1363, 1548
BmsI	GCATC	2 cut(s)	373, 1381
BpiI	GAAGAC	1 cut(s)	878
BpmI	CTGGAG	1 cut(s)	1460
Bpu10I	CCTNAGC	1 cut(s)	1128
Bpu1102I	GCTNAGC	1 cut(s)	951
Bpu14I	TTCGAA	1 cut(s)	560
BpuEI	CTTGAG	4 cut(s)	320, 538, 851, 1177
BpuMI	CCSGG	5 cut(s)	325, 544, 545, 1363, 1548
BsaBI	GATNNNNATC	3 cut(s)	447, 1203, 1395
BsaHI	GRCGYC	1 cut(s)	24
BsaI	GGTCTC	3 cut(s)	314, 538, 1226
BsaWI	WCCGGW	1 cut(s)	116
BsaXI	ACNNNNNCTCC	2 cut(s)	497, 527
Bse118I	RCCGGY	1 cut(s)	168
Bse3DI	GCAATG	1 cut(s)	779
Bse8I	GATNNNNATC	3 cut(s)	447, 1203, 1395
BseBI	CCWGG	3 cut(s)	95, 200, 1042
BseGI	GGATG	5 cut(s)	448, 571, 1251, 1639, 1660
BseJI	GATNNNNATC	3 cut(s)	447, 1203, 1395
BseMI	GCAATG	1 cut(s)	779
BseMII	CTCAG	6 cut(s)	671, 942, 1047, 1104, 1363, 1455
BseRI	GAGGAG	2 cut(s)	38, 65
BseXI	GCAGC	4 cut(s)	196, 1297, 1378, 1682
BseYI	CCCAGC	3 cut(s)	1004, 1064, 1334
Bsh1285I	CGRYCG	2 cut(s)	64, 1201
BshFI	GGCC	4 cut(s)	159, 1277, 1361, 1462
BshNI	GGYRCC	2 cut(s)	1045, 1101
BsiEI	CGRYCG	2 cut(s)	64, 1201
BsiHKAI	GWGCWC	2 cut(s)	1503, 1515
BsiHKCI	CYCGRG	1 cut(s)	543
BsiSI	CCGG	6 cut(s)	117, 169, 325, 544, 1363, 1547
BsmAI	GTCTC	4 cut(s)	314, 538, 1008, 1226
BsmI	GAATGC	1 cut(s)	1118
BsnI	GGCC	4 cut(s)	159, 1277, 1361, 1462
Bso31I	GGTCTC	3 cut(s)	314, 538, 1226
BsoBI	CYCGRG	1 cut(s)	543
Bsp119I	TTCGAA	1 cut(s)	560
Bsp1286I	GDGCHC	2 cut(s)	1503, 1515
Bsp1407I	TGTACA	1 cut(s)	985
Bsp143I	GATC	6 cut(s)	102, 1152, 1198, 1204, 1264, 1588
Bsp1720I	GCTNAGC	1 cut(s)	951
BspACI	CCGC	8 cut(s)	57, 79, 144, 156, 166, 461, 890, 1338
BspANI	GGCC	4 cut(s)	159, 1277, 1361, 1462
BspCNI	CTCAG	6 cut(s)	670, 943, 1048, 1103, 1362, 1456
BspLI	GGNNCC	6 cut(s)	160, 1039, 1047, 1103, 1387, 1545
BspMAI	CTGCAG	1 cut(s)	1287
BspPI	GGATC	1 cut(s)	1259
BspQI	GCTCTTC	1 cut(s)	1404
BspT104I	TTCGAA	1 cut(s)	560
BspT107I	GGYRCC	2 cut(s)	1045, 1101
BspTNI	GGTCTC	3 cut(s)	314, 538, 1226
BsrDI	GCAATG	1 cut(s)	779
BsrFI	RCCGGY	1 cut(s)	168
BsrGI	TGTACA	1 cut(s)	985
BssAI	RCCGGY	1 cut(s)	168
BssMI	GATC	6 cut(s)	102, 1152, 1198, 1204, 1264, 1588
BssNAI	GTATAC	1 cut(s)	1481
BssNI	GRCGYC	1 cut(s)	24
BssSI	CACGAG	2 cut(s)	148, 1496
BssT1I	CCWWGG	4 cut(s)	290, 1241, 1268, 1665
Bst1107I	GTATAC	1 cut(s)	1481
Bst2BI	CACGAG	2 cut(s)	148, 1496
Bst2UI	CCWGG	3 cut(s)	95, 200, 1042
Bst4CI	ACNGT	4 cut(s)	803, 985, 990, 1037
Bst6I	CTCTTC	5 cut(s)	247, 732, 900, 1404, 1469
BstACI	GRCGYC	1 cut(s)	24
BstAPI	GCANNNNNTGC	3 cut(s)	1078, 1313, 1578
BstAUI	TGTACA	1 cut(s)	985
BstBI	TTCGAA	1 cut(s)	560
BstC8I	GCNNGC	5 cut(s)	817, 957, 1062, 1355, 1359
BstDEI	CTNAG	7 cut(s)	657, 951, 1056, 1090, 1128, 1349, 1464
BstDSI	CCRYGG	3 cut(s)	433, 495, 1644
BstENI	CCTNNNNNAGG	1 cut(s)	737
BstF5I	GGATG	5 cut(s)	448, 571, 1251, 1639, 1660
BstH2I	RGCGCY	1 cut(s)	918
BstHHI	GCGC	3 cut(s)	829, 917, 1290
BstKTI	GATC	6 cut(s)	105, 1155, 1201, 1207, 1267, 1591
BstMAI	GTCTC	4 cut(s)	314, 538, 1008, 1226
BstMBI	GATC	6 cut(s)	102, 1152, 1198, 1204, 1264, 1588
BstMCI	CGRYCG	2 cut(s)	64, 1201
BstNI	CCWGG	3 cut(s)	95, 200, 1042
BstSCI	CCNGG	8 cut(s)	93, 198, 323, 542, 543, 1040, 1361, 1546
BstSFI	CTRYAG	1 cut(s)	1283
BstV1I	GCAGC	4 cut(s)	196, 1297, 1378, 1682
BstV2I	GAAGAC	1 cut(s)	878
BstZ17I	GTATAC	1 cut(s)	1481
BsuI	GTATCC	1 cut(s)	266
BsuRI	GGCC	4 cut(s)	159, 1277, 1361, 1462
BtgI	CCRYGG	3 cut(s)	433, 495, 1644
BtgZI	GCGATG	1 cut(s)	1710
BtsCI	GGATG	5 cut(s)	448, 571, 1251, 1639, 1660
Cac8I	GCNNGC	5 cut(s)	817, 957, 1062, 1355, 1359
CfoI	GCGC	3 cut(s)	829, 917, 1290
Cfr10I	RCCGGY	1 cut(s)	168
Cfr13I	GGNCC	5 cut(s)	158, 430, 1276, 1360, 1560
Cfr9I	CCCGGG	1 cut(s)	543
CseI	GACGC	1 cut(s)	13
CsiI	ACCWGGT	1 cut(s)	93
Csp6I	GTAC	2 cut(s)	986, 1046
CspCI	CAANNNNNGTGG	2 cut(s)	168, 203
CviAII	CATG	8 cut(s)	642, 677, 771, 1110, 1123, 1237, 1382, 1487
CviQI	GTAC	2 cut(s)	986, 1046
DdeI	CTNAG	7 cut(s)	657, 951, 1056, 1090, 1128, 1349, 1464
DpnI	GATC	6 cut(s)	104, 1154, 1200, 1206, 1266, 1590
DpnII	GATC	6 cut(s)	102, 1152, 1198, 1204, 1264, 1588
DraIII	CACNNNGTG	1 cut(s)	439
DrdI	GACNNNNNNGTC	1 cut(s)	988
DriI	GACNNNNNGTC	1 cut(s)	23
DseDI	GACNNNNNNGTC	1 cut(s)	988
Eam1104I	CTCTTC	5 cut(s)	247, 732, 900, 1404, 1469
Eam1105I	GACNNNNNGTC	1 cut(s)	23
EarI	CTCTTC	5 cut(s)	247, 732, 900, 1404, 1469
EciI	GGCGGA	2 cut(s)	46, 133
Ecl136II	GAGCTC	1 cut(s)	1501
Eco130I	CCWWGG	4 cut(s)	290, 1241, 1268, 1665
Eco24I	GRGCYC	1 cut(s)	1503
Eco31I	GGTCTC	3 cut(s)	314, 538, 1226
Eco32I	GATATC	1 cut(s)	723
Eco47I	GGWCC	2 cut(s)	430, 1560
Eco53kI	GAGCTC	1 cut(s)	1501
Eco57I	CTGAAG	2 cut(s)	707, 939
Eco88I	CYCGRG	1 cut(s)	543
EcoICRI	GAGCTC	1 cut(s)	1501
EcoNI	CCTNNNNNAGG	1 cut(s)	737
EcoO109I	RGGNCCY	1 cut(s)	1276
EcoRI	GAATTC	1 cut(s)	1223
EcoRII	CCWGG	3 cut(s)	93, 198, 1040
EcoRV	GATATC	1 cut(s)	723
EcoT14I	CCWWGG	4 cut(s)	290, 1241, 1268, 1665
EcoT38I	GRGCYC	1 cut(s)	1503
ErhI	CCWWGG	4 cut(s)	290, 1241, 1268, 1665
FaeI	CATG	8 cut(s)	645, 680, 774, 1113, 1126, 1240, 1385, 1490
FalI	AAGNNNNNCTT	4 cut(s)	329, 361, 1591, 1623
FatI	CATG	8 cut(s)	641, 676, 770, 1109, 1122, 1236, 1381, 1486
FbaI	TGATCA	1 cut(s)	1204
FblI	GTMKAC	1 cut(s)	1480
Fnu4HI	GCNGC	7 cut(s)	157, 166, 210, 891, 1286, 1367, 1671
FokI	GGATG	5 cut(s)	455, 578, 1238, 1646, 1667
FriOI	GRGCYC	1 cut(s)	1503
Fsp4HI	GCNGC	7 cut(s)	157, 166, 210, 891, 1286, 1367, 1671
FspBI	CTAG	3 cut(s)	728, 843, 848
GlaI	GCGC	3 cut(s)	828, 916, 1289
GluI	GCNGC	7 cut(s)	157, 166, 210, 891, 1286, 1367, 1671
GsaI	CCCAGC	3 cut(s)	1008, 1068, 1338
GsuI	CTGGAG	1 cut(s)	1460
HaeII	RGCGCY	1 cut(s)	918
HaeIII	GGCC	4 cut(s)	159, 1277, 1361, 1462
HapII	CCGG	6 cut(s)	117, 169, 325, 544, 1363, 1547
HgaI	GACGC	1 cut(s)	13
HhaI	GCGC	3 cut(s)	829, 917, 1290
Hin1I	GRCGYC	1 cut(s)	24
Hin1II	CATG	8 cut(s)	645, 680, 774, 1113, 1126, 1240, 1385, 1490
Hin6I	GCGC	3 cut(s)	827, 915, 1288
HinP1I	GCGC	3 cut(s)	827, 915, 1288
HindIII	AAGCTT	1 cut(s)	1130
HinfI	GANTC	4 cut(s)	260, 301, 361, 508
HpaII	CCGG	6 cut(s)	117, 169, 325, 544, 1363, 1547
HphI	GGTGA	3 cut(s)	166, 188, 1396
Hpy166II	GTNNAC	5 cut(s)	174, 196, 456, 933, 1481
Hpy188I	TCNGA	6 cut(s)	247, 313, 589, 1057, 1152, 1344
Hpy188III	TCNNGA	4 cut(s)	32, 478, 728, 1202
Hpy8I	GTNNAC	5 cut(s)	174, 196, 456, 933, 1481
Hpy99I	CGWCG	1 cut(s)	17
HpyCH4III	ACNGT	4 cut(s)	803, 985, 990, 1037
HpyCH4V	TGCA	6 cut(s)	645, 1118, 1172, 1285, 1316, 1572
HpyF3I	CTNAG	7 cut(s)	657, 951, 1056, 1090, 1128, 1349, 1464
Hsp92I	GRCGYC	1 cut(s)	24
Hsp92II	CATG	8 cut(s)	645, 680, 774, 1113, 1126, 1240, 1385, 1490
HspAI	GCGC	3 cut(s)	827, 915, 1288
KpnI	GGTACC	1 cut(s)	1049
Ksp22I	TGATCA	1 cut(s)	1204
Kzo9I	GATC	6 cut(s)	102, 1152, 1198, 1204, 1264, 1588
LguI	GCTCTTC	1 cut(s)	1404
LmnI	GCTCC	3 cut(s)	1385, 1518, 1543
Lsp1109I	GCAGC	4 cut(s)	196, 1297, 1378, 1682
LweI	GCATC	2 cut(s)	373, 1381
MabI	ACCWGGT	1 cut(s)	93
MaeI	CTAG	3 cut(s)	728, 843, 848
MaeIII	GTNAC	3 cut(s)	25, 990, 1688
MalI	GATC	6 cut(s)	104, 1154, 1200, 1206, 1266, 1590
MboI	GATC	6 cut(s)	102, 1152, 1198, 1204, 1264, 1588
MfeI	CAATTG	1 cut(s)	1424
MhlI	GDGCHC	2 cut(s)	1503, 1515
MluCI	AATT	9 cut(s)	420, 472, 650, 855, 962, 1223, 1292, 1308, 1424
MmeI	TCCRAC	1 cut(s)	589
MroXI	GAANNNNTTC	1 cut(s)	759
MseI	TTAA	1 cut(s)	789
MslI	CAYNNNNRTG	3 cut(s)	885, 1508, 1524
MspA1I	CMGCKG	1 cut(s)	1338
MspI	CCGG	6 cut(s)	117, 169, 325, 544, 1363, 1547
MspR9I	CCNGG	8 cut(s)	95, 200, 325, 544, 545, 1042, 1363, 1548
MunI	CAATTG	1 cut(s)	1424
Mva1269I	GAATGC	1 cut(s)	1118
MvaI	CCWGG	3 cut(s)	95, 200, 1042
NciI	CCSGG	5 cut(s)	325, 544, 545, 1363, 1548
NdeII	GATC	6 cut(s)	102, 1152, 1198, 1204, 1264, 1588
NlaIII	CATG	8 cut(s)	645, 680, 774, 1113, 1126, 1240, 1385, 1490
NlaIV	GGNNCC	6 cut(s)	160, 1039, 1047, 1103, 1387, 1545
NmeAIII	GCCGAG	1 cut(s)	1699
NmuCI	GTSAC	3 cut(s)	25, 990, 1688
NspV	TTCGAA	1 cut(s)	560
PciSI	GCTCTTC	1 cut(s)	1404
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 57
PctI	GAATGC	1 cut(s)	1118
PdmI	GAANNNNTTC	1 cut(s)	759
PfeI	GAWTC	4 cut(s)	260, 301, 361, 508
PflMI	CCANNNNNTGG	3 cut(s)	205, 439, 1242
PkrI	GCNGC	7 cut(s)	158, 167, 211, 892, 1287, 1368, 1672
Ple19I	CGATCG	1 cut(s)	1201
PsiI	TTATAA	2 cut(s)	854, 1136
Psp124BI	GAGCTC	1 cut(s)	1503
Psp6I	CCWGG	3 cut(s)	93, 198, 1040
PspFI	CCCAGC	3 cut(s)	1004, 1064, 1334
PspGI	CCWGG	3 cut(s)	93, 198, 1040
PspN4I	GGNNCC	6 cut(s)	160, 1039, 1047, 1103, 1387, 1545
PspPI	GGNCC	5 cut(s)	158, 430, 1276, 1360, 1560
PsrI	GAACNNNNNNTAC	2 cut(s)	969, 1001
PstI	CTGCAG	1 cut(s)	1287
PvuI	CGATCG	1 cut(s)	1201
RsaI	GTAC	2 cut(s)	987, 1047
RsaNI	GTAC	2 cut(s)	986, 1046
RseI	CAYNNNNRTG	3 cut(s)	885, 1508, 1524
SacI	GAGCTC	1 cut(s)	1503
SapI	GCTCTTC	1 cut(s)	1404
SaqAI	TTAA	1 cut(s)	789
SatI	GCNGC	7 cut(s)	157, 166, 210, 891, 1286, 1367, 1671
Sau3AI	GATC	6 cut(s)	102, 1152, 1198, 1204, 1264, 1588
Sau96I	GGNCC	5 cut(s)	158, 430, 1276, 1360, 1560
ScrFI	CCNGG	8 cut(s)	95, 200, 325, 544, 545, 1042, 1363, 1548
SduI	GDGCHC	2 cut(s)	1503, 1515
SexAI	ACCWGGT	1 cut(s)	93
SfaNI	GCATC	2 cut(s)	373, 1381
SfcI	CTRYAG	1 cut(s)	1283
SfuI	TTCGAA	1 cut(s)	560
SinI	GGWCC	2 cut(s)	430, 1560
SmaI	CCCGGG	1 cut(s)	545
SmiMI	CAYNNNNRTG	3 cut(s)	885, 1508, 1524
SmlI	CTYRAG	4 cut(s)	335, 517, 830, 1192
SmoI	CTYRAG	4 cut(s)	335, 517, 830, 1192
SpeI	ACTAGT	1 cut(s)	847
Sse9I	AATT	9 cut(s)	420, 472, 650, 855, 962, 1223, 1292, 1308, 1424
SsiI	CCGC	8 cut(s)	57, 79, 144, 156, 166, 461, 890, 1338
SspMI	CTAG	3 cut(s)	728, 843, 848
SstI	GAGCTC	1 cut(s)	1503
StyD4I	CCNGG	8 cut(s)	93, 198, 323, 542, 543, 1040, 1361, 1546
StyI	CCWWGG	4 cut(s)	290, 1241, 1268, 1665
TaaI	ACNGT	4 cut(s)	803, 985, 990, 1037
TaqI	TCGA	5 cut(s)	299, 560, 972, 1176, 1587
TaqII	GACCGA	1 cut(s)	78
TasI	AATT	9 cut(s)	420, 472, 650, 855, 962, 1223, 1292, 1308, 1424
TatI	WGTACW	1 cut(s)	985
TauI	GCSGC	3 cut(s)	159, 168, 893
TfiI	GAWTC	4 cut(s)	260, 301, 361, 508
Tru1I	TTAA	1 cut(s)	789
Tru9I	TTAA	1 cut(s)	789
TseFI	GTSAC	3 cut(s)	25, 990, 1688
TseI	GCWGC	4 cut(s)	209, 1285, 1366, 1670
Tsp45I	GTSAC	3 cut(s)	25, 990, 1688
TspDTI	ATGAA	3 cut(s)	226, 485, 1111
TspGWI	ACGGA	3 cut(s)	30, 441, 918
TspMI	CCCGGG	1 cut(s)	543
Van91I	CCANNNNNTGG	3 cut(s)	205, 439, 1242
VpaK11BI	GGWCC	2 cut(s)	430, 1560
XagI	CCTNNNNNAGG	1 cut(s)	737
XapI	RAATTY	4 cut(s)	472, 650, 1223, 1292
XbaI	TCTAGA	1 cut(s)	727
XcmI	CCANNNNNNNNNTGG	1 cut(s)	440
XmaI	CCCGGG	1 cut(s)	543
XmiI	GTMKAC	1 cut(s)	1480
XmnI	GAANNNNTTC	1 cut(s)	759
XspI	CTAG	3 cut(s)	728, 843, 848

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.