Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0001775

Physical Location & Seq

Genomic Coordinates Reverse (-)

61826783 .. 61829005

Gene Structure 2223 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G034910.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGGCTCACGACAACGGTGACGGCGTTACTCACGACCTGTTCTCGCTGCTCGCCTCCGGTGATCGGGACTTCCTCCTCCGTAACAACGGCGACCAGGTAAAGATCAATACTTTGACTGGTAAGATTTTGGGGTTGTATTTCTCTGGCTCATGGTGTGGTCCATGTCGTCGTTTCACCCCAAACTTGGTGGAGGTCTACCAGGACCTTGCTTCCAAAGGGGACTTTGAAGTTGTGTTTGTCTCTTCTGATAGAGATGAGGAGTCATTCAGTGGCTACTTCTCTGAAATGCCGTGGCTTGCGGTTCCCTTTTCGGATTTGGAGACCCGAAAAGGCCTGAAAGACTTGTTCAAAGTCAGGGGAATTCCTCATCTTGTTGTTATTGATGCCAATGGGAAGGTTTCCACTGATAAAGGGACTGTAGTTGTGAGGGAGTATGGTGTAGAGGGGTATCCGTTCACCGCGGAGAAGATTAGTCTCTTGAAAGAGCAAGAGGAGGCGGCTAAGAAGAATCAGTCCTTGAGTTCTCTCTTGGTTTCTAATTCTCGGGATTACTTGGTTTCGAGTGATGGGAATAAGGTGTCTGTGTCTGAGCTTGAAGGAAAGATAGTTGGTCTGTATTTCTCACTGAATATTCACAAGCCATGCAAAGACTTTACTCAGACACTTCTGAAGTTCTATAAGAGTCTCAAGGAGAAAGGAGAGAACTTTGAGATAGTGTTGGTATCTTTAGACTATGAAGAAGAACACTTCAAAGAAGGGATTGCAGTCCCATGGTTGGCATTGCCATTTAAGGACAAGAACTGCAAAAAACTGGCTCGCTACTTTGAGCTTGAGACAGTACCAACACTAGTTATAATTGGGCAAGATGGAAAGACCTTACACCCCAATGTGACTGAACTTATTGAAGAGCATGGGATTGAAGCCTACCCATTTTCAGCAGAGAAGGTTGCTGAGCTTGCTGAGATTGAGAAGGCCAAACTCGAAGCACAGACTCTGGAGTCGCTTTTGGTGACTGAAGACTTAGACTTTGTGATAGAGACGAGCGGTGCCAAGGTACCGGTGTCTGAGCTTGTTGGGAAGCACATTCTGCTCTATTTCTCAGCTCATTGGTGTCCCCCATGTCGTTCATTCCTGCCTAAGCTCATAGCAGCATATCACGAGATCAAGGCCAAGGACAATGCATTTGAAATCATCTTTATCTCAAGTGATCGTGATCAATCCTCCTTCGATGACTTCTTTTCGAGCATGCCTTGGTTAGCCCTCCCATTTGGTGATCCAAGGAAGATGTTCCTGCAGCGCAAATTCAAAATCCAAGGCATTCCTGCTGTGGTAGCTATCAGCCCCACTGGCAAGACACTCACCACGACAGCCCGAAAGCTTATACTAGCTCATGGTGCTGATGCTTTTCCATTCACTGAAGAGCATTTGAAGCATTTGGAAGAAAAGGTAGACGAAGAAGCAAAGGGGTGGCCTGAGAAAGTGAAGAGTGAGCTACATGTTGAACATGAACTTGCACGCACTCGTTGCCACGAATATGTGTGTAATGGGTGTAGGGAGTCCGGAAGTGGTTGGTCTTTTTCCTGTAAGAAGTGTGACTTTGATCTTCACCCACAGTGTGCTCTGAAGAACAATGAAGCAACAAAGGATGATCCTGAGACCAAGGCAAAGGAGGGATATGTTTGTGATGGTGACGTGTGCCGTAAAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0003824 GO:0004791 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006935 GO:0008150 GO:0008152 GO:0009605 GO:0009636 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010035 GO:0010038 GO:0010183 GO:0010769 GO:0015036 GO:0016043 GO:0016049 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0022414 GO:0022603 GO:0022604 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0040008 GO:0040011 GO:0042221 GO:0042330 GO:0043900 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0045595 GO:0046686 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050918 GO:0051128 GO:0051239 GO:0051510 GO:0051704 GO:0051716 GO:0055114 GO:0060284 GO:0060560 GO:0065007 GO:0070887 GO:0071840 GO:0080092 GO:0097237 GO:0098754 GO:0098869 GO:1990748 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C1_2 Thioredoxin_8

Protein Analysis

569

Amino Acids

63.9

Weight (kDa)

5.47

Isoelectric Point (pI)

32.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AhpC-TSA	PF00578	25 - 134	6.1e-08	AhpC/TSA family
Redoxin	PF08534	27 - 135	2.3e-07	Redoxin
Thioredoxin_2	PF13098	39 - 132	4.8e-06	Thioredoxin-like domain
Thioredoxin_8	PF13905	40 - 133	3.2e-28	Thioredoxin-like
Thioredoxin_8	PF13905	200 - 291	5.6e-23	Thioredoxin-like
AhpC-TSA	PF00578	347 - 414	5.8e-08	AhpC/TSA family
Thioredoxin_8	PF13905	359 - 451	2.2e-26	Thioredoxin-like
Thioredoxin_2	PF13098	359 - 449	3.3e-06	Thioredoxin-like domain
C1_2	PF03107	499 - 540	1.3e-06	C1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70480 AT1G70480
fragaria_vesca	FvH4_4g34241 FvH4_4g34250 FvH4_4g34260 FvH4_4g34260 FvH4_4g34260 FvH4_4g34260
malus_domestica	MD13G1093300.v1.1 MD13G1093500.v1.1 MD16G1094500.v1.1 MD16G1094600.v1.1 MD16G1094700.v1.1
prunus_persica	Prupe.1G251900_v2.0.a1 Prupe.1G252000_v2.0.a1
pyrus_communis	pycom13g08130 pycom13g08140 pycom16g08030 pycom16g08040 pycom16g08050
rosa_chinensis	RchiOBHm_Chr4g0443711 RchiOBHm_Chr6g0250421
rosa_laevigata	RLG00000005898 RLG00000005899 RLG00000005900
rosa_multiflora	Rmu_co8463221.1_g000001 Rmu_sc0001426.1_g000010 Rmu_sc0001426.1_g000011 Rmu_sc0001426.1_g000013 Rmu_sc0008739.1_g000001
rosa_roxburghii	Rroxscaffold_5G00384250 Rroxscaffold_5G00384260 Rroxscaffold_5G00384270
rosa_rugosa	Rorug05G0496400 Rorug05G0496500
rosa_samantha	Rh4AG406400 Rh4AG406500 Rh4AG406600 Rh4BG417400 Rh4BG417500 Rh4BG417600 Rh4BG417700 Rh4CG431900 Rh4DG413000
rosa_wichuraiana	Rw4G034900 Rw4G034910 Rw4G034920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	854
AasI	GACNNNNNNGTC	1 cut(s)	997
Acc65I	GGTACC	1 cut(s)	1054
AccB1I	GGYRCC	2 cut(s)	1046, 1054
AccBSI	CCGCTC	1 cut(s)	1044
AccI	GTMKAC	2 cut(s)	195, 1449
AccII	CGCG	1 cut(s)	461
AccIII	TCCGGA	1 cut(s)	1559
AciI	CCGC	5 cut(s)	299, 459, 461, 497, 1044
AclWI	GGATC	2 cut(s)	1268, 1643
AcsI	RAATTY	2 cut(s)	360, 1301
AcuI	CTGAAG	4 cut(s)	689, 1035, 1437, 1643
AdeI	CACNNNGTG	1 cut(s)	1616
AfaI	GTAC	2 cut(s)	840, 1056
AfiI	CCNNNNNNNGG	3 cut(s)	63, 184, 775
AflIII	ACRYGT	2 cut(s)	1495, 1692
AgeI	ACCGGT	1 cut(s)	1057
AhlI	ACTAGT	1 cut(s)	847
AjiI	CACGTC	1 cut(s)	1693
AjnI	CCWGG	2 cut(s)	93, 198
Alw21I	GWGCWC	1 cut(s)	1621
Alw26I	GTCTC	7 cut(s)	244, 314, 479, 689, 827, 1031, 1649
AlwI	GGATC	2 cut(s)	1268, 1643
AlwNI	CAGNNNCTG	1 cut(s)	994
Ama87I	CYCGRG	1 cut(s)	543
Aor13HI	TCCGGA	1 cut(s)	1559
AoxI	GGCC	4 cut(s)	331, 972, 1167, 1469
ApeKI	GCWGC	3 cut(s)	46, 1148, 1294
ApoI	RAATTY	2 cut(s)	360, 1301
ArsI	GACNNNNNNTTYG	4 cut(s)	1010, 1042, 1166, 1198
AsiGI	ACCGGT	1 cut(s)	1057
Asp700I	GAANNNNTTC	1 cut(s)	1286
Asp718I	GGTACC	1 cut(s)	1054
AspLEI	GCGC	1 cut(s)	1299
AspS9I	GGNCC	2 cut(s)	158, 202
AsuHPI	GGTGA	9 cut(s)	29, 71, 166, 448, 1021, 1283, 1351, 1598, 1700
AvaI	CYCGRG	1 cut(s)	543
AvaII	GGWCC	2 cut(s)	158, 202
BaeI	ACNNNNGTAYC	2 cut(s)	1046, 1079
BanI	GGYRCC	2 cut(s)	1046, 1054
BauI	CACGAG	1 cut(s)	1157
BbsI	GAAGAC	1 cut(s)	1023
Bbv12I	GWGCWC	1 cut(s)	1621
BbvI	GCAGC	3 cut(s)	33, 1160, 1306
BccI	CCATC	3 cut(s)	560, 860, 1679
BceAI	ACGGC	4 cut(s)	37, 103, 274, 1683
BciT130I	CCWGG	2 cut(s)	95, 200
BciVI	GTATCC	1 cut(s)	459
BclI	TGATCA	1 cut(s)	1213
BcoDI	GTCTC	7 cut(s)	244, 314, 479, 689, 827, 1031, 1649
BcuI	ACTAGT	1 cut(s)	847
BfaI	CTAG	2 cut(s)	848, 1385
BfmI	CTRYAG	2 cut(s)	417, 1292
BfuI	GTATCC	1 cut(s)	459
BglI	GCCNNNNNGGC	1 cut(s)	1347
BisI	GCNGC	4 cut(s)	47, 498, 1149, 1295
BlpI	GCTNAGC	1 cut(s)	951
BlsI	GCNGC	4 cut(s)	48, 499, 1150, 1296
Bme1390I	CCNGG	2 cut(s)	95, 200
Bme18I	GGWCC	2 cut(s)	158, 202
BmeT110I	CYCGRG	1 cut(s)	543
BmgBI	CACGTC	1 cut(s)	1693
BmgT120I	GGNCC	2 cut(s)	158, 202
BmiI	GGNNCC	3 cut(s)	303, 1048, 1056
BmrFI	CCNGG	2 cut(s)	95, 200
BmsI	GCATC	2 cut(s)	373, 1390
BpiI	GAAGAC	1 cut(s)	1023
BpmI	CTGGAG	1 cut(s)	1016
Bpu10I	CCTNAGC	1 cut(s)	1137
Bpu1102I	GCTNAGC	1 cut(s)	951
BpuEI	CTTGAG	4 cut(s)	538, 671, 851, 1186
BsaBI	GATNNNNATC	1 cut(s)	1212
BsaI	GGTCTC	2 cut(s)	314, 1649
BsaJI	CCNNGG	9 cut(s)	290, 459, 770, 1050, 1170, 1250, 1277, 1312, 1659
BsaWI	WCCGGW	3 cut(s)	56, 1057, 1559
Bsc4I	CCNNNNNNNGG	3 cut(s)	63, 184, 775
Bse118I	RCCGGY	1 cut(s)	1057
Bse1I	ACTGG	3 cut(s)	121, 816, 1351
Bse3DI	GCAATG	1 cut(s)	779
Bse8I	GATNNNNATC	1 cut(s)	1212
BseAI	TCCGGA	1 cut(s)	1559
BseBI	CCWGG	2 cut(s)	95, 200
BseDI	CCNNGG	9 cut(s)	290, 459, 770, 1050, 1170, 1250, 1277, 1312, 1659
BseGI	GGATG	1 cut(s)	1651
BseJI	GATNNNNATC	1 cut(s)	1212
BseLI	CCNNNNNNNGG	3 cut(s)	63, 184, 775
BseMI	GCAATG	1 cut(s)	779
BseMII	CTCAG	8 cut(s)	579, 671, 942, 951, 1056, 1113, 1464, 1644
BseNI	ACTGG	3 cut(s)	121, 816, 1351
BseRI	GAGGAG	3 cut(s)	65, 272, 506
BseXI	GCAGC	3 cut(s)	33, 1160, 1306
Bsh1236I	CGCG	1 cut(s)	461
BshFI	GGCC	4 cut(s)	333, 974, 1169, 1471
BshNI	GGYRCC	2 cut(s)	1046, 1054
BshTI	ACCGGT	1 cut(s)	1057
BsiHKAI	GWGCWC	1 cut(s)	1621
BsiHKCI	CYCGRG	1 cut(s)	543
BsiSI	CCGG	3 cut(s)	57, 1058, 1560
BslFI	GGGAC	5 cut(s)	80, 233, 427, 752, 1098
BslI	CCNNNNNNNGG	3 cut(s)	63, 184, 775
BsmAI	GTCTC	7 cut(s)	244, 314, 479, 689, 827, 1031, 1649
BsmBI	CGTCTC	1 cut(s)	1031
BsmFI	GGGAC	5 cut(s)	80, 233, 427, 752, 1098
BsmI	GAATGC	1 cut(s)	1317
BsnI	GGCC	4 cut(s)	333, 974, 1169, 1471
Bso31I	GGTCTC	2 cut(s)	314, 1649
BsoBI	CYCGRG	1 cut(s)	543
Bsp1286I	GDGCHC	1 cut(s)	1621
Bsp13I	TCCGGA	1 cut(s)	1559
Bsp143I	GATC	8 cut(s)	61, 102, 1161, 1207, 1213, 1273, 1600, 1648
Bsp1720I	GCTNAGC	1 cut(s)	951
Bsp19I	CCATGG	1 cut(s)	770
BspACI	CCGC	5 cut(s)	299, 459, 461, 497, 1044
BspANI	GGCC	4 cut(s)	333, 974, 1169, 1471
BspCNI	CTCAG	8 cut(s)	580, 670, 943, 952, 1057, 1112, 1465, 1645
BspEI	TCCGGA	1 cut(s)	1559
BspFNI	CGCG	1 cut(s)	461
BspLI	GGNNCC	3 cut(s)	303, 1048, 1056
BspMAI	CTGCAG	1 cut(s)	1296
BspPI	GGATC	2 cut(s)	1268, 1643
BspQI	GCTCTTC	2 cut(s)	900, 1413
BspT107I	GGYRCC	2 cut(s)	1046, 1054
BspTNI	GGTCTC	2 cut(s)	314, 1649
BsrBI	CCGCTC	1 cut(s)	1044
BsrDI	GCAATG	1 cut(s)	779
BsrFI	RCCGGY	1 cut(s)	1057
BsrI	ACTGG	3 cut(s)	121, 816, 1351
BssAI	RCCGGY	1 cut(s)	1057
BssECI	CCNNGG	9 cut(s)	290, 459, 770, 1050, 1170, 1250, 1277, 1312, 1659
BssMI	GATC	8 cut(s)	61, 102, 1161, 1207, 1213, 1273, 1600, 1648
BssSI	CACGAG	1 cut(s)	1157
BssT1I	CCWWGG	7 cut(s)	770, 1050, 1170, 1250, 1277, 1312, 1659
Bst2BI	CACGAG	1 cut(s)	1157
Bst2UI	CCWGG	2 cut(s)	95, 200
Bst4CI	ACNGT	4 cut(s)	17, 418, 838, 1614
Bst6I	CTCTTC	4 cut(s)	247, 900, 1413, 1478
BstAPI	GCANNNNNTGC	2 cut(s)	1087, 1524
BstC8I	GCNNGC	6 cut(s)	51, 297, 817, 957, 1247, 1516
BstDSI	CCRYGG	3 cut(s)	290, 459, 770
BstF5I	GGATG	1 cut(s)	1651
BstFNI	CGCG	1 cut(s)	461
BstHHI	GCGC	1 cut(s)	1299
BstKTI	GATC	8 cut(s)	64, 105, 1164, 1210, 1216, 1276, 1603, 1651
BstMAI	GTCTC	7 cut(s)	244, 314, 479, 689, 827, 1031, 1649
BstMBI	GATC	8 cut(s)	61, 102, 1161, 1207, 1213, 1273, 1600, 1648
BstMWI	GCNNNNNNNGC	7 cut(s)	956, 1087, 1331, 1347, 1394, 1429, 1524
BstNI	CCWGG	2 cut(s)	95, 200
BstNSI	RCATGY	2 cut(s)	1249, 1499
BstSCI	CCNGG	2 cut(s)	93, 198
BstSFI	CTRYAG	2 cut(s)	417, 1292
BstUI	CGCG	1 cut(s)	461
BstV1I	GCAGC	3 cut(s)	33, 1160, 1306
BstV2I	GAAGAC	1 cut(s)	1023
BsuI	GTATCC	1 cut(s)	459
BsuRI	GGCC	4 cut(s)	333, 974, 1169, 1471
BtgI	CCRYGG	3 cut(s)	290, 459, 770
BtrI	CACGTC	1 cut(s)	1693
BtsCI	GGATG	1 cut(s)	1651
BtsIMutI	CAGTG	6 cut(s)	274, 402, 623, 1344, 1413, 1619
Cac8I	GCNNGC	6 cut(s)	51, 297, 817, 957, 1247, 1516
CaiI	CAGNNNCTG	1 cut(s)	994
CfoI	GCGC	1 cut(s)	1299
Cfr10I	RCCGGY	1 cut(s)	1057
Cfr13I	GGNCC	2 cut(s)	158, 202
Cfr42I	CCGCGG	1 cut(s)	462
CsiI	ACCWGGT	1 cut(s)	93
Csp6I	GTAC	2 cut(s)	839, 1055
CspAI	ACCGGT	1 cut(s)	1057
CspCI	CAANNNNNGTGG	2 cut(s)	168, 203
CviQI	GTAC	2 cut(s)	839, 1055
DpnI	GATC	8 cut(s)	63, 104, 1163, 1209, 1215, 1275, 1602, 1650
DpnII	GATC	8 cut(s)	61, 102, 1161, 1207, 1213, 1273, 1600, 1648
DraIII	CACNNNGTG	1 cut(s)	1616
DrdI	GACNNNNNNGTC	1 cut(s)	997
DseDI	GACNNNNNNGTC	1 cut(s)	997
Eam1104I	CTCTTC	4 cut(s)	247, 900, 1413, 1478
EarI	CTCTTC	4 cut(s)	247, 900, 1413, 1478
Eco130I	CCWWGG	7 cut(s)	770, 1050, 1170, 1250, 1277, 1312, 1659
Eco147I	AGGCCT	1 cut(s)	333
Eco31I	GGTCTC	2 cut(s)	314, 1649
Eco47I	GGWCC	2 cut(s)	158, 202
Eco57I	CTGAAG	4 cut(s)	689, 1035, 1437, 1643
Eco88I	CYCGRG	1 cut(s)	543
EcoO109I	RGGNCCY	1 cut(s)	202
EcoRI	GAATTC	1 cut(s)	360
EcoRII	CCWGG	2 cut(s)	93, 198
EcoT14I	CCWWGG	7 cut(s)	770, 1050, 1170, 1250, 1277, 1312, 1659
EcoT22I	ATGCAT	1 cut(s)	1183
ErhI	CCWWGG	7 cut(s)	770, 1050, 1170, 1250, 1277, 1312, 1659
Esp3I	CGTCTC	1 cut(s)	1031
FalI	AAGNNNNNCTT	2 cut(s)	1580, 1612
FaqI	GGGAC	5 cut(s)	80, 233, 427, 752, 1098
FbaI	TGATCA	1 cut(s)	1213
FblI	GTMKAC	2 cut(s)	195, 1449
Fnu4HI	GCNGC	4 cut(s)	47, 498, 1149, 1295
FokI	GGATG	1 cut(s)	1658
Fsp4HI	GCNGC	4 cut(s)	47, 498, 1149, 1295
FspBI	CTAG	2 cut(s)	848, 1385
GlaI	GCGC	1 cut(s)	1298
GluI	GCNGC	4 cut(s)	47, 498, 1149, 1295
GsuI	CTGGAG	1 cut(s)	1016
HaeIII	GGCC	4 cut(s)	333, 974, 1169, 1471
HapII	CCGG	3 cut(s)	57, 1058, 1560
HhaI	GCGC	1 cut(s)	1299
Hin6I	GCGC	1 cut(s)	1297
HinP1I	GCGC	1 cut(s)	1297
HindIII	AAGCTT	2 cut(s)	1376, 1704
HinfI	GANTC	6 cut(s)	260, 508, 682, 991, 998, 1556
HpaII	CCGG	3 cut(s)	57, 1058, 1560
HphI	GGTGA	9 cut(s)	29, 71, 166, 448, 1021, 1283, 1351, 1598, 1700
Hpy166II	GTNNAC	3 cut(s)	196, 456, 1450
Hpy188I	TCNGA	8 cut(s)	247, 283, 313, 589, 660, 669, 1066, 1623
Hpy8I	GTNNAC	3 cut(s)	196, 456, 1450
Hpy99I	CGWCG	1 cut(s)	171
HpyAV	CCTTC	6 cut(s)	388, 590, 749, 937, 964, 1234
HpyCH4III	ACNGT	4 cut(s)	17, 418, 838, 1614
HpyCH4IV	ACGT	1 cut(s)	1692
HpyCH4V	TGCA	6 cut(s)	645, 764, 804, 1181, 1294, 1514
HpyF10VI	GCNNNNNNNGC	7 cut(s)	956, 1087, 1331, 1347, 1394, 1429, 1524
HpySE526I	ACGT	1 cut(s)	1692
HspAI	GCGC	1 cut(s)	1297
Kpn2I	TCCGGA	1 cut(s)	1559
KpnI	GGTACC	1 cut(s)	1058
Ksp22I	TGATCA	1 cut(s)	1213
KspI	CCGCGG	1 cut(s)	462
Kzo9I	GATC	8 cut(s)	61, 102, 1161, 1207, 1213, 1273, 1600, 1648
LguI	GCTCTTC	2 cut(s)	900, 1413
Lsp1109I	GCAGC	3 cut(s)	33, 1160, 1306
LweI	GCATC	2 cut(s)	373, 1390
MabI	ACCWGGT	1 cut(s)	93
MaeI	CTAG	2 cut(s)	848, 1385
MaeII	ACGT	1 cut(s)	1692
MaeIII	GTNAC	7 cut(s)	17, 25, 80, 889, 1009, 1592, 1688
MalI	GATC	8 cut(s)	63, 104, 1163, 1209, 1215, 1275, 1602, 1650
MbiI	CCGCTC	1 cut(s)	1044
MboI	GATC	8 cut(s)	61, 102, 1161, 1207, 1213, 1273, 1600, 1648
MhlI	GDGCHC	1 cut(s)	1621
MluCI	AATT	4 cut(s)	360, 538, 855, 1301
MlyI	GAGTC	5 cut(s)	269, 691, 985, 1007, 1565
Mph1103I	ATGCAT	1 cut(s)	1183
MroI	TCCGGA	1 cut(s)	1559
MroXI	GAANNNNTTC	1 cut(s)	1286
MseI	TTAA	1 cut(s)	789
MslI	CAYNNNNRTG	2 cut(s)	885, 1533
MspA1I	CMGCKG	1 cut(s)	461
MspI	CCGG	3 cut(s)	57, 1058, 1560
MspR9I	CCNGG	2 cut(s)	95, 200
Mva1269I	GAATGC	1 cut(s)	1317
MvaI	CCWGG	2 cut(s)	95, 200
MvnI	CGCG	1 cut(s)	461
MwoI	GCNNNNNNNGC	7 cut(s)	956, 1087, 1331, 1347, 1394, 1429, 1524
NcoI	CCATGG	1 cut(s)	770
NdeII	GATC	8 cut(s)	61, 102, 1161, 1207, 1213, 1273, 1600, 1648
NlaIV	GGNNCC	3 cut(s)	303, 1048, 1056
NmuCI	GTSAC	5 cut(s)	17, 889, 1009, 1592, 1688
NsiI	ATGCAT	1 cut(s)	1183
NspI	RCATGY	2 cut(s)	1249, 1499
PaeI	GCATGC	1 cut(s)	1249
PceI	AGGCCT	1 cut(s)	333
PciI	ACATGT	1 cut(s)	1495
PciSI	GCTCTTC	2 cut(s)	900, 1413
PcsI	WCGNNNNNNNCGW	1 cut(s)	21
PctI	GAATGC	1 cut(s)	1317
PdmI	GAANNNNTTC	1 cut(s)	1286
PfeI	GAWTC	1 cut(s)	508
PinAI	ACCGGT	1 cut(s)	1057
PkrI	GCNGC	4 cut(s)	48, 499, 1150, 1296
PleI	GAGTC	5 cut(s)	268, 690, 985, 1006, 1564
PpsI	GAGTC	5 cut(s)	268, 690, 985, 1006, 1564
PpuMI	RGGWCCY	1 cut(s)	202
PscI	ACATGT	1 cut(s)	1495
PsiI	TTATAA	1 cut(s)	854
Psp5II	RGGWCCY	1 cut(s)	202
Psp6I	CCWGG	2 cut(s)	93, 198
PspGI	CCWGG	2 cut(s)	93, 198
PspN4I	GGNNCC	3 cut(s)	303, 1048, 1056
PspPI	GGNCC	2 cut(s)	158, 202
PspPPI	RGGWCCY	1 cut(s)	202
PstI	CTGCAG	1 cut(s)	1296
PstNI	CAGNNNCTG	1 cut(s)	994
RsaI	GTAC	2 cut(s)	840, 1056
RsaNI	GTAC	2 cut(s)	839, 1055
RseI	CAYNNNNRTG	2 cut(s)	885, 1533
SacII	CCGCGG	1 cut(s)	462
SapI	GCTCTTC	2 cut(s)	900, 1413
SaqAI	TTAA	1 cut(s)	789
SatI	GCNGC	4 cut(s)	47, 498, 1149, 1295
Sau3AI	GATC	8 cut(s)	61, 102, 1161, 1207, 1213, 1273, 1600, 1648
Sau96I	GGNCC	2 cut(s)	158, 202
SchI	GAGTC	5 cut(s)	269, 691, 985, 1007, 1565
ScrFI	CCNGG	2 cut(s)	95, 200
SduI	GDGCHC	1 cut(s)	1621
SexAI	ACCWGGT	1 cut(s)	93
SfaNI	GCATC	2 cut(s)	373, 1390
SfcI	CTRYAG	2 cut(s)	417, 1292
Sfr303I	CCGCGG	1 cut(s)	462
SgrBI	CCGCGG	1 cut(s)	462
SinI	GGWCC	2 cut(s)	158, 202
SmiMI	CAYNNNNRTG	2 cut(s)	885, 1533
SmlI	CTYRAG	4 cut(s)	517, 686, 830, 1201
SmoI	CTYRAG	4 cut(s)	517, 686, 830, 1201
SpeI	ACTAGT	1 cut(s)	847
SphI	GCATGC	1 cut(s)	1249
Sse9I	AATT	4 cut(s)	360, 538, 855, 1301
SseBI	AGGCCT	1 cut(s)	333
SsiI	CCGC	5 cut(s)	299, 459, 461, 497, 1044
SspI	AATATT	1 cut(s)	631
SspMI	CTAG	2 cut(s)	848, 1385
StuI	AGGCCT	1 cut(s)	333
StyD4I	CCNGG	2 cut(s)	93, 198
StyI	CCWWGG	7 cut(s)	770, 1050, 1170, 1250, 1277, 1312, 1659
TaaI	ACNGT	4 cut(s)	17, 418, 838, 1614
TaiI	ACGT	1 cut(s)	1695
TaqI	TCGA	4 cut(s)	560, 981, 1227, 1241
TasI	AATT	4 cut(s)	360, 538, 855, 1301
TauI	GCSGC	1 cut(s)	500
TfiI	GAWTC	1 cut(s)	508
Tru1I	TTAA	1 cut(s)	789
Tru9I	TTAA	1 cut(s)	789
TscAI	CASTG	6 cut(s)	274, 409, 630, 1351, 1420, 1619
TseFI	GTSAC	5 cut(s)	17, 889, 1009, 1592, 1688
TseI	GCWGC	3 cut(s)	46, 1148, 1294
Tsp45I	GTSAC	5 cut(s)	17, 889, 1009, 1592, 1688
TspDTI	ATGAA	4 cut(s)	750, 1116, 1521, 1647
TspGWI	ACGGA	2 cut(s)	68, 441
TspRI	CASTG	6 cut(s)	274, 409, 630, 1351, 1420, 1619
VpaK11BI	GGWCC	2 cut(s)	158, 202
XapI	RAATTY	2 cut(s)	360, 1301
XceI	RCATGY	2 cut(s)	1249, 1499
XmiI	GTMKAC	2 cut(s)	195, 1449
XmnI	GAANNNNTTC	1 cut(s)	1286
XspI	CTAG	2 cut(s)	848, 1385
Zsp2I	ATGCAT	1 cut(s)	1183

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.