MD13G1093500.v1.1

Domain of unknown function DUF220

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001775
Physical Location & Seq
Forward (+)
6585027 .. 6596595
11569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1093500.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGCTCCGTAATCAATCGCTTAAAGTTGTCCTTTCAATGGGTGAGACAGAGGTGACCAAGACAAGCGTTAACATCGACAAGAACAAGAGAAATGGGCACCTCAAGCCCAAGGCCTTTGATCTGGCGTTTTCTAAAGCTTTGTTGCAGGTTCCTCACAGGCTGCAAAATTGCCTAAAGTCCCAGCTCAAGAGACTAGCAAAGGATGATGGAGTGAAAAAGGTGAGCTCTGTTCCTAAAAAGGCTGCATCTTTGGAGGATGACCTCGAGAAGCAGTTGCAAGCATGGAGAGAAAATCCTTCTTGGGTTGATCAACCACCGTATATAAAGGTCAGTGTACCAAAAGGTTCTCTTTGCAACCTGAGTGTAAACGTCAATGTTGGTTTACCCCCAGACGCAGTATATAATATTGTAACTGACCCCGACAATAAGAGGGTTTTCAAAAATATTAAGGAAGTGATATCAAGAAACGTTTTGGTTGATGAAGGGCATAGGCAGGTGGTTGATCTGGAGCAGGCAGCTATATGGAAGTTTCTTTGGTGGTCAGGGACCATTGCGGTTCGTGTTATAGTAGATCAAAACAGAAAAGACCACTCAATGAAGTTCAAGCAAGTCAAAACAGGGTTTATGGAGAAATTTGAAGGCTACTGGAGAGTCGAACCTCTTTTTGTCGATGAAAAAACCTGCTTTCCGTTTAAACCCAGGACATGGGCAGATTATAATTCTTGCACAGGAGGCAAAGGAAGGATTGGTTCAAGGGTGAGCTTGGAGCAACTGATCCAACCAACTATAGTTCCACCTCCACCGATTTCATGGTACCTAAGGGGAATCACCAGCAAGACTACTGAGATGCTGATAAACGACATGCTTGCGGAAGCTGCCAGATTACGAGGAGAATTAAATCCTGGAGAGTCCAATAAAGAGATTGAAATGTCTCAGGACGGGCAAGTACTTAGTCATGTTGATAATGTGTCTAACATCAAAGAAAGATGGGCCCTTCATAGGAGGAACGCAAAGCAATGCCATCGGAGGCTACCGCCTGTCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF220

Protein Analysis

349

Amino Acids

39.7

Weight (kDa)

9.64

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF220 PF02713 181 - 252 3.7e-31 Domain of unknown function DUF220
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 717
AarI CACCTGC 1 cut(s) 484
Acc36I ACCTGC 3 cut(s) 136, 484, 689
Acc65I GGTACC 1 cut(s) 813
AccB1I GGYRCC 2 cut(s) 96, 813
AccB7I CCANNNNNTGG 1 cut(s) 705
AciI CCGC 3 cut(s) 554, 869, 1034
AclI AACGTT 1 cut(s) 468
AclWI GGATC 1 cut(s) 769
AcsI RAATTY 1 cut(s) 632
AfaI GTAC 3 cut(s) 336, 815, 948
AfiI CCNNNNNNNGG 3 cut(s) 37, 705, 999
AgsI TTSAA 6 cut(s) 36, 439, 604, 638, 753, 926
AjnI CCWGG 2 cut(s) 698, 901
AluBI AGCT 6 cut(s) 137, 184, 225, 518, 762, 875
AluI AGCT 6 cut(s) 137, 184, 225, 518, 762, 875
Alw21I GWGCWC 1 cut(s) 227
Alw26I GTCTC 3 cut(s) 38, 184, 936
AlwI GGATC 1 cut(s) 769
Ama87I CYCGRG 1 cut(s) 263
AoxI GGCC 2 cut(s) 111, 990
ApaI GGGCCC 1 cut(s) 994
ApeKI GCWGC 4 cut(s) 160, 242, 515, 875
ApoI RAATTY 1 cut(s) 632
Asp718I GGTACC 1 cut(s) 813
AspS9I GGNCC 3 cut(s) 546, 990, 991
AsuHPI GGTGA 5 cut(s) 53, 64, 232, 769, 820
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 1 cut(s) 546
AxyI CCTNAGG 1 cut(s) 818
BaeGI GKGCMC 2 cut(s) 99, 994
BanI GGYRCC 2 cut(s) 96, 813
BanII GRGCYC 2 cut(s) 227, 994
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 4 cut(s) 147, 229, 527, 862
BccI CCATC 3 cut(s) 200, 981, 1029
BcgI CGANNNNNNTGC 4 cut(s) 848, 882, 1004, 1038
BciT130I CCWGG 2 cut(s) 700, 903
BclI TGATCA 1 cut(s) 307
BcoDI GTCTC 3 cut(s) 38, 184, 936
BfaI CTAG 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 786
BfuAI ACCTGC 3 cut(s) 136, 484, 689
BisI GCNGC 4 cut(s) 161, 243, 516, 876
BlsI GCNGC 4 cut(s) 162, 244, 517, 877
BmcAI AGTACT 1 cut(s) 948
Bme1390I CCNGG 2 cut(s) 700, 903
Bme18I GGWCC 1 cut(s) 546
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 3 cut(s) 546, 990, 991
BmiI GGNNCC 5 cut(s) 98, 150, 547, 815, 992
BmrFI CCNGG 2 cut(s) 700, 903
BmsI GCATC 2 cut(s) 254, 837
BpmI CTGGAG 3 cut(s) 527, 667, 924
BpuEI CTTGAG 2 cut(s) 86, 170
BsaJI CCNNGG 2 cut(s) 108, 698
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 705, 999
Bse1I ACTGG 1 cut(s) 650
Bse21I CCTNAGG 1 cut(s) 818
Bse3DI GCAATG 2 cut(s) 549, 1022
BseBI CCWGG 2 cut(s) 700, 903
BseDI CCNNGG 2 cut(s) 108, 698
BseGI GGATG 2 cut(s) 208, 262
BseLI CCNNNNNNNGG 3 cut(s) 37, 705, 999
BseMI GCAATG 2 cut(s) 549, 1022
BseMII CTCAG 3 cut(s) 350, 834, 947
BseNI ACTGG 1 cut(s) 650
BseRI GAGGAG 1 cut(s) 903
BseSI GKGCMC 2 cut(s) 99, 994
BseXI GCAGC 4 cut(s) 147, 229, 527, 862
BseYI CCCAGC 1 cut(s) 180
BshFI GGCC 2 cut(s) 113, 992
BshNI GGYRCC 2 cut(s) 96, 813
BsiHKAI GWGCWC 1 cut(s) 227
BsiHKCI CYCGRG 1 cut(s) 263
BslFI GGGAC 2 cut(s) 163, 559
BslI CCNNNNNNNGG 3 cut(s) 37, 705, 999
BsmAI GTCTC 3 cut(s) 38, 184, 936
BsmFI GGGAC 2 cut(s) 163, 559
BsnI GGCC 2 cut(s) 113, 992
BsoBI CYCGRG 1 cut(s) 263
Bsp120I GGGCCC 1 cut(s) 990
Bsp1286I GDGCHC 3 cut(s) 99, 227, 994
Bsp143I GATC 5 cut(s) 118, 307, 502, 571, 774
BspACI CCGC 3 cut(s) 554, 869, 1034
BspANI GGCC 2 cut(s) 113, 992
BspCNI CTCAG 3 cut(s) 351, 835, 946
BspLI GGNNCC 5 cut(s) 98, 150, 547, 815, 992
BspMI ACCTGC 3 cut(s) 136, 484, 689
BspPI GGATC 1 cut(s) 769
BspT107I GGYRCC 2 cut(s) 96, 813
BsrDI GCAATG 2 cut(s) 549, 1022
BsrI ACTGG 1 cut(s) 650
BssECI CCNNGG 2 cut(s) 108, 698
BssMI GATC 5 cut(s) 118, 307, 502, 571, 774
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 2 cut(s) 700, 903
Bst4CI ACNGT 1 cut(s) 318
BstC8I GCNNGC 3 cut(s) 279, 513, 867
BstDEI CTNAG 5 cut(s) 359, 818, 843, 933, 950
BstEII GGTNACC 1 cut(s) 52
BstF5I GGATG 2 cut(s) 208, 262
BstKTI GATC 5 cut(s) 121, 310, 505, 574, 777
BstMAI GTCTC 3 cut(s) 38, 184, 936
BstMBI GATC 5 cut(s) 118, 307, 502, 571, 774
BstMWI GCNNNNNNNGC 3 cut(s) 103, 732, 875
BstNI CCWGG 2 cut(s) 700, 903
BstNSI RCATGY 1 cut(s) 865
BstPI GGTNACC 1 cut(s) 52
BstSCI CCNGG 2 cut(s) 698, 901
BstSFI CTRYAG 1 cut(s) 786
BstSLI GKGCMC 2 cut(s) 99, 994
BstV1I GCAGC 4 cut(s) 147, 229, 527, 862
Bsu36I CCTNAGG 1 cut(s) 818
BsuRI GGCC 2 cut(s) 113, 992
BtsCI GGATG 2 cut(s) 208, 262
BtsIMutI CAGTG 1 cut(s) 337
BveI ACCTGC 3 cut(s) 136, 484, 689
Cac8I GCNNGC 3 cut(s) 279, 513, 867
Cfr13I GGNCC 3 cut(s) 546, 990, 991
CseI GACGC 1 cut(s) 401
Csp6I GTAC 3 cut(s) 335, 814, 947
CviAII CATG 5 cut(s) 282, 705, 810, 862, 956
CviQI GTAC 3 cut(s) 335, 814, 947
DdeI CTNAG 5 cut(s) 359, 818, 843, 933, 950
DpnI GATC 5 cut(s) 120, 309, 504, 573, 776
DpnII GATC 5 cut(s) 118, 307, 502, 571, 774
DraI TTTAAA 1 cut(s) 694
Ecl136II GAGCTC 1 cut(s) 225
Eco130I CCWWGG 1 cut(s) 108
Eco147I AGGCCT 1 cut(s) 113
Eco24I GRGCYC 2 cut(s) 227, 994
Eco32I GATATC 1 cut(s) 459
Eco47I GGWCC 1 cut(s) 546
Eco53kI GAGCTC 1 cut(s) 225
Eco81I CCTNAGG 1 cut(s) 818
Eco88I CYCGRG 1 cut(s) 263
Eco91I GGTNACC 1 cut(s) 52
EcoICRI GAGCTC 1 cut(s) 225
EcoO109I RGGNCCY 1 cut(s) 991
EcoO65I GGTNACC 1 cut(s) 52
EcoRII CCWGG 2 cut(s) 698, 901
EcoRV GATATC 1 cut(s) 459
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 2 cut(s) 227, 994
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 5 cut(s) 285, 708, 813, 865, 959
FalI AAGNNNNNCTT 4 cut(s) 15, 47, 333, 365
FaqI GGGAC 2 cut(s) 163, 559
FatI CATG 5 cut(s) 281, 704, 809, 861, 955
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 4 cut(s) 161, 243, 516, 876
FokI GGATG 2 cut(s) 215, 269
FriOI GRGCYC 2 cut(s) 227, 994
Fsp4HI GCNGC 4 cut(s) 161, 243, 516, 876
FspBI CTAG 1 cut(s) 194
GluI GCNGC 4 cut(s) 161, 243, 516, 876
GsaI CCCAGC 1 cut(s) 184
GsuI CTGGAG 3 cut(s) 527, 667, 924
HaeIII GGCC 2 cut(s) 113, 992
HgaI GACGC 1 cut(s) 401
Hin1II CATG 5 cut(s) 285, 708, 813, 865, 959
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 3 cut(s) 651, 825, 908
HpaI GTTAAC 1 cut(s) 70
HphI GGTGA 5 cut(s) 53, 64, 232, 769, 820
Hpy166II GTNNAC 4 cut(s) 70, 335, 367, 383
Hpy188I TCNGA 1 cut(s) 1026
Hpy188III TCNNGA 5 cut(s) 187, 265, 462, 506, 935
Hpy8I GTNNAC 4 cut(s) 70, 335, 367, 383
HpyAV CCTTC 5 cut(s) 306, 476, 632, 735, 1004
HpyCH4III ACNGT 1 cut(s) 318
HpyCH4IV ACGT 2 cut(s) 369, 468
HpyCH4V TGCA 6 cut(s) 145, 163, 245, 277, 354, 726
HpyF10VI GCNNNNNNNGC 3 cut(s) 103, 732, 875
HpyF3I CTNAG 5 cut(s) 359, 818, 843, 933, 950
HpySE526I ACGT 2 cut(s) 369, 468
Hsp92II CATG 5 cut(s) 285, 708, 813, 865, 959
KpnI GGTACC 1 cut(s) 817
Ksp22I TGATCA 1 cut(s) 307
KspAI GTTAAC 1 cut(s) 70
Kzo9I GATC 5 cut(s) 118, 307, 502, 571, 774
LmnI GCTCC 3 cut(s) 9, 508, 766
Lsp1109I GCAGC 4 cut(s) 147, 229, 527, 862
LweI GCATC 2 cut(s) 254, 837
MaeI CTAG 1 cut(s) 194
MaeII ACGT 2 cut(s) 369, 468
MaeIII GTNAC 2 cut(s) 52, 409
MalI GATC 5 cut(s) 120, 309, 504, 573, 776
MboI GATC 5 cut(s) 118, 307, 502, 571, 774
MhlI GDGCHC 3 cut(s) 99, 227, 994
MluCI AATT 4 cut(s) 166, 632, 718, 893
MlyI GAGTC 2 cut(s) 660, 917
MmeI TCCRAC 1 cut(s) 802
MseI TTAA 5 cut(s) 21, 69, 447, 693, 896
MspR9I CCNGG 2 cut(s) 700, 903
MssI GTTTAAAC 1 cut(s) 694
MvaI CCWGG 2 cut(s) 700, 903
MwoI GCNNNNNNNGC 3 cut(s) 103, 732, 875
NdeII GATC 5 cut(s) 118, 307, 502, 571, 774
NlaIII CATG 5 cut(s) 285, 708, 813, 865, 959
NlaIV GGNNCC 5 cut(s) 98, 150, 547, 815, 992
NmuCI GTSAC 1 cut(s) 52
NspI RCATGY 1 cut(s) 865
PaeR7I CTCGAG 1 cut(s) 263
PaqCI CACCTGC 1 cut(s) 484
PceI AGGCCT 1 cut(s) 113
PfeI GAWTC 1 cut(s) 825
PflMI CCANNNNNTGG 1 cut(s) 705
PfoI TCCNGGA 1 cut(s) 901
PkrI GCNGC 4 cut(s) 162, 244, 517, 877
PleI GAGTC 2 cut(s) 659, 916
PmeI GTTTAAAC 1 cut(s) 694
PpsI GAGTC 2 cut(s) 659, 916
PsiI TTATAA 1 cut(s) 717
Psp124BI GAGCTC 1 cut(s) 227
Psp1406I AACGTT 1 cut(s) 468
Psp6I CCWGG 2 cut(s) 698, 901
PspEI GGTNACC 1 cut(s) 52
PspFI CCCAGC 1 cut(s) 180
PspGI CCWGG 2 cut(s) 698, 901
PspN4I GGNNCC 5 cut(s) 98, 150, 547, 815, 992
PspOMI GGGCCC 1 cut(s) 990
PspPI GGNCC 3 cut(s) 546, 990, 991
RsaI GTAC 3 cut(s) 336, 815, 948
RsaNI GTAC 3 cut(s) 335, 814, 947
SacI GAGCTC 1 cut(s) 227
SaqAI TTAA 5 cut(s) 21, 69, 447, 693, 896
SatI GCNGC 4 cut(s) 161, 243, 516, 876
Sau3AI GATC 5 cut(s) 118, 307, 502, 571, 774
Sau96I GGNCC 3 cut(s) 546, 990, 991
ScaI AGTACT 1 cut(s) 948
SchI GAGTC 2 cut(s) 660, 917
ScrFI CCNGG 2 cut(s) 700, 903
SduI GDGCHC 3 cut(s) 99, 227, 994
SfaNI GCATC 2 cut(s) 254, 837
SfcI CTRYAG 1 cut(s) 786
Sfr274I CTCGAG 1 cut(s) 263
SinI GGWCC 1 cut(s) 546
SlaI CTCGAG 1 cut(s) 263
SmlI CTYRAG 3 cut(s) 101, 185, 263
SmoI CTYRAG 3 cut(s) 101, 185, 263
Sse9I AATT 4 cut(s) 166, 632, 718, 893
SseBI AGGCCT 1 cut(s) 113
SsiI CCGC 3 cut(s) 554, 869, 1034
SspI AATATT 2 cut(s) 406, 445
SspMI CTAG 1 cut(s) 194
SstI GAGCTC 1 cut(s) 227
StuI AGGCCT 1 cut(s) 113
StyD4I CCNGG 2 cut(s) 698, 901
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 1 cut(s) 318
TaiI ACGT 2 cut(s) 372, 471
TaqI TCGA 4 cut(s) 75, 264, 654, 669
TasI AATT 4 cut(s) 166, 632, 718, 893
TatI WGTACW 1 cut(s) 946
TfiI GAWTC 1 cut(s) 825
Tru1I TTAA 5 cut(s) 21, 69, 447, 693, 896
Tru9I TTAA 5 cut(s) 21, 69, 447, 693, 896
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 4 cut(s) 160, 242, 515, 875
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 5 cut(s) 495, 611, 687, 798, 986
TspGWI ACGGA 1 cut(s) 678
TspRI CASTG 1 cut(s) 337
Van91I CCANNNNNTGG 1 cut(s) 705
VpaK11BI GGWCC 1 cut(s) 546
XapI RAATTY 1 cut(s) 632
XceI RCATGY 1 cut(s) 865
XcmI CCANNNNNNNNNTGG 1 cut(s) 807
XhoI CTCGAG 1 cut(s) 263
XspI CTAG 1 cut(s) 194
ZrmI AGTACT 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.