Rroxscaffold_5G00384250

Domain of unknown function DUF220

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001775
Physical Location & Seq
Reverse (-)
63722566 .. 63725377
2812 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00384250.1

Sequence Viewer

Length: 1110 bp
ATGGGTGAGACAGAGGTGCCCACGACAAGCATAGACATCCGGAAGAACGAGAAAAATGGGCGCCTCAAGGCATTGGATGTAGTGCTCTCTAAAGCTTTTGTCCAGGTTCCTCACAAGCTACAGAATTTTCTTAAGTCACAGTTTCGAAGACTACCAAAAGCTGATGGAGTTAAAACAGTGAGCTCTGTCCCTAAAAAGGCTTCATCTTTGGAGGTTGACCTGGAAAAGCAGTTGCAAGCATGGAGAGAAAATCCTTCATGGGATGATCAAACACCACATATAAAGTTTGAACTATCAGTACATCTGGGAAGGAACATTTCAAGAATTTATGGCTTCTCTGAGTATGTAGTAACCGTGAGTCACAAAGTAGTTTACTCTGTGCAGGTCAGTGTGCCAAAAGGTTCTCTCTGCAACCTCAGTGTAAACGTCAATGTTGGGTTGCCCCCAGATGCAGTATATAATATTGTAACTGACCCCGACAATAAGAGGGTTTTCAAGAATATTAAGGCAAGTAATATTGAGGTGATATCCAGAAGAGTTTTGGTTGACGAAGGTCTTAGGCAGGTTGTTGATCTAGAGCAAGCAGCTATATGGAAATTTCTTTGGTGGTCAGGGACCATTGCAGTTCATGTTTTAGTAGATCAAAACAGAGAAGACCACTCAATGAAGTTCAAGCAAGTCAAAACAGGGTTTATGGAGAGATTTGAAGGCTGTTGGAGAGTGGAGCCTCTTTTTGTTGATGAAAAAACCTGCTTTCCCTATAAACCCACAACATGGGCAGACTATAATTCACACACTGGAGGCAAAGGAAGGATCGGATCAAAGGTGAGCTTGGACCAACTGATCCAACCGTCTATTGTTCCTCCCCCACCCATTTCTTGGTATCTGAGGGGTATCACCGCCAAGACCACAGAGATGTTGATAAATGATCTTCTTGCTGAAGCCGCCAGAATACGGGGAGAATTAAACCCTGAGAAGTCTGATAAAGAGGTTGAAATGTCTGAGGAAGTACTTAATCAAGTTGATAACACCTGTAACATCAAAGAAAGATGGGCTATGCATAGGAGAAATGCAAAGCAATGTCATCGGAGGCTACCGACTGCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF220

Protein Analysis

369

Amino Acids

42.08

Weight (kDa)

9.25

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF220 PF02713 204 - 275 5.7e-29 Domain of unknown function DUF220
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 373, 553, 758
AccB1I GGYRCC 2 cut(s) 16, 60
AccB7I CCANNNNNTGG 2 cut(s) 774, 879
AccIII TCCGGA 1 cut(s) 39
AciI CCGC 2 cut(s) 900, 945
AclWI GGATC 3 cut(s) 821, 826, 838
AcsI RAATTY 3 cut(s) 124, 324, 596
AcuI CTGAAG 1 cut(s) 960
AcyI GRCGYC 1 cut(s) 61
AfaI GTAC 2 cut(s) 300, 1011
AfiI CCNNNNNNNGG 4 cut(s) 196, 774, 879, 954
AflII CTTAAG 1 cut(s) 131
AgsI TTSAA 6 cut(s) 290, 321, 496, 673, 707, 995
AjnI CCWGG 2 cut(s) 102, 219
AluBI AGCT 6 cut(s) 95, 118, 161, 183, 587, 831
AluI AGCT 6 cut(s) 95, 118, 161, 183, 587, 831
Alw21I GWGCWC 2 cut(s) 87, 185
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 3 cut(s) 821, 826, 838
Aor13HI TCCGGA 1 cut(s) 39
ApeKI GCWGC 1 cut(s) 584
ApoI RAATTY 3 cut(s) 124, 324, 596
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 2 cut(s) 615, 835
AsuHPI GGTGA 4 cut(s) 17, 535, 838, 889
AsuII TTCGAA 1 cut(s) 145
AvaII GGWCC 2 cut(s) 615, 835
BaeGI GKGCMC 1 cut(s) 21
BanI GGYRCC 2 cut(s) 16, 60
BanII GRGCYC 1 cut(s) 185
BbsI GAAGAC 2 cut(s) 154, 660
Bbv12I GWGCWC 2 cut(s) 87, 185
BbvI GCAGC 1 cut(s) 596
BccI CCATC 2 cut(s) 158, 1044
BcgI CGANNNNNNTGC 2 cut(s) 1067, 1101
BciT130I CCWGG 2 cut(s) 104, 221
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 1 cut(s) 2
BfaI CTAG 1 cut(s) 575
BfmI CTRYAG 1 cut(s) 119
BfoI RGCGCY 1 cut(s) 64
BfrI CTTAAG 1 cut(s) 131
BfuAI ACCTGC 3 cut(s) 373, 553, 758
BisI GCNGC 2 cut(s) 585, 945
BlsI GCNGC 2 cut(s) 586, 946
BmcAI AGTACT 1 cut(s) 1011
Bme1390I CCNGG 2 cut(s) 104, 221
Bme18I GGWCC 2 cut(s) 615, 835
BmgT120I GGNCC 2 cut(s) 615, 835
BmiI GGNNCC 5 cut(s) 18, 62, 108, 616, 726
BmrFI CCNGG 2 cut(s) 104, 221
BmsI GCATC 1 cut(s) 439
BoxI GACNNNNGTC 1 cut(s) 552
BpiI GAAGAC 2 cut(s) 154, 660
BpmI CTGGAG 1 cut(s) 819
Bpu14I TTCGAA 1 cut(s) 145
BpuEI CTTGAG 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 61
BsaWI WCCGGW 1 cut(s) 39
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 774, 879, 954
Bse1I ACTGG 1 cut(s) 802
Bse3DI GCAATG 2 cut(s) 618, 1085
BseAI TCCGGA 1 cut(s) 39
BseBI CCWGG 2 cut(s) 104, 221
BseGI GGATG 3 cut(s) 36, 82, 268
BseLI CCNNNNNNNGG 4 cut(s) 196, 774, 879, 954
BseMI GCAATG 2 cut(s) 618, 1085
BseMII CTCAG 5 cut(s) 330, 430, 878, 963, 993
BseNI ACTGG 1 cut(s) 802
BseSI GKGCMC 1 cut(s) 21
BseXI GCAGC 1 cut(s) 596
BsgI GTGCAG 1 cut(s) 401
BshNI GGYRCC 2 cut(s) 16, 60
BsiHKAI GWGCWC 2 cut(s) 87, 185
BsiSI CCGG 1 cut(s) 40
BslFI GGGAC 2 cut(s) 173, 628
BslI CCNNNNNNNGG 4 cut(s) 196, 774, 879, 954
BsmAI GTCTC 1 cut(s) 2
BsmFI GGGAC 2 cut(s) 173, 628
Bsp119I TTCGAA 1 cut(s) 145
Bsp1286I GDGCHC 3 cut(s) 21, 87, 185
Bsp13I TCCGGA 1 cut(s) 39
Bsp143I GATC 7 cut(s) 265, 571, 640, 813, 818, 843, 928
BspACI CCGC 2 cut(s) 900, 945
BspCNI CTCAG 5 cut(s) 331, 429, 879, 964, 994
BspEI TCCGGA 1 cut(s) 39
BspLI GGNNCC 5 cut(s) 18, 62, 108, 616, 726
BspMI ACCTGC 3 cut(s) 373, 553, 758
BspPI GGATC 3 cut(s) 821, 826, 838
BspT104I TTCGAA 1 cut(s) 145
BspT107I GGYRCC 2 cut(s) 16, 60
BspTI CTTAAG 1 cut(s) 131
BsrDI GCAATG 2 cut(s) 618, 1085
BsrI ACTGG 1 cut(s) 802
BssMI GATC 7 cut(s) 265, 571, 640, 813, 818, 843, 928
BssNI GRCGYC 1 cut(s) 61
Bst2UI CCWGG 2 cut(s) 104, 221
Bst4CI ACNGT 4 cut(s) 141, 178, 355, 852
Bst6I CTCTTC 1 cut(s) 529
BstACI GRCGYC 1 cut(s) 61
BstAFI CTTAAG 1 cut(s) 131
BstBI TTCGAA 1 cut(s) 145
BstC8I GCNNGC 2 cut(s) 237, 582
BstDEI CTNAG 6 cut(s) 339, 416, 557, 887, 972, 1002
BstF5I GGATG 3 cut(s) 36, 82, 268
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 7 cut(s) 268, 574, 643, 816, 821, 846, 931
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 7 cut(s) 265, 571, 640, 813, 818, 843, 928
BstMWI GCNNNNNNNGC 1 cut(s) 944
BstNI CCWGG 2 cut(s) 104, 221
BstPAI GACNNNNGTC 1 cut(s) 552
BstSCI CCNGG 2 cut(s) 102, 219
BstSFI CTRYAG 1 cut(s) 119
BstSLI GKGCMC 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 596
BstV2I GAAGAC 2 cut(s) 154, 660
BtsCI GGATG 3 cut(s) 36, 82, 268
BtsIMutI CAGTG 4 cut(s) 183, 394, 424, 795
BveI ACCTGC 3 cut(s) 373, 553, 758
Cac8I GCNNGC 2 cut(s) 237, 582
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 2 cut(s) 615, 835
Csp6I GTAC 2 cut(s) 299, 1010
CviAII CATG 4 cut(s) 240, 258, 629, 774
CviQI GTAC 2 cut(s) 299, 1010
DdeI CTNAG 6 cut(s) 339, 416, 557, 887, 972, 1002
DinI GGCGCC 1 cut(s) 62
DpnI GATC 7 cut(s) 267, 573, 642, 815, 820, 845, 930
DpnII GATC 7 cut(s) 265, 571, 640, 813, 818, 843, 928
Eam1104I CTCTTC 1 cut(s) 529
EarI CTCTTC 1 cut(s) 529
Ecl136II GAGCTC 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 185
Eco32I GATATC 1 cut(s) 528
Eco47I GGWCC 2 cut(s) 615, 835
Eco53kI GAGCTC 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 960
EcoICRI GAGCTC 1 cut(s) 183
EcoRII CCWGG 2 cut(s) 102, 219
EcoRV GATATC 1 cut(s) 528
EcoT22I ATGCAT 1 cut(s) 1062
EcoT38I GRGCYC 1 cut(s) 185
EgeI GGCGCC 1 cut(s) 62
EheI GGCGCC 1 cut(s) 62
FaeI CATG 4 cut(s) 243, 261, 632, 777
FalI AAGNNNNNCTT 2 cut(s) 815, 847
FaqI GGGAC 2 cut(s) 173, 628
FatI CATG 4 cut(s) 239, 257, 628, 773
FbaI TGATCA 1 cut(s) 265
Fnu4HI GCNGC 2 cut(s) 585, 945
FokI GGATG 3 cut(s) 23, 89, 275
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 2 cut(s) 585, 945
FspBI CTAG 1 cut(s) 575
GlaI GCGC 1 cut(s) 62
GluI GCNGC 2 cut(s) 585, 945
GsuI CTGGAG 1 cut(s) 819
HaeII RGCGCY 1 cut(s) 64
HapII CCGG 1 cut(s) 40
HhaI GCGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 61
Hin1II CATG 4 cut(s) 243, 261, 632, 777
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 2 cut(s) 217, 547
HindII GTYRAC 2 cut(s) 217, 547
HindIII AAGCTT 1 cut(s) 93
HinfI GANTC 1 cut(s) 358
HpaII CCGG 1 cut(s) 40
HphI GGTGA 4 cut(s) 17, 535, 838, 889
Hpy166II GTNNAC 4 cut(s) 217, 373, 424, 547
Hpy188I TCNGA 6 cut(s) 340, 818, 888, 982, 1003, 1089
Hpy188III TCNNGA 5 cut(s) 40, 321, 496, 531, 575
Hpy8I GTNNAC 4 cut(s) 217, 373, 424, 547
HpyAV CCTTC 5 cut(s) 264, 303, 545, 701, 804
HpyCH4III ACNGT 4 cut(s) 141, 178, 355, 852
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 7 cut(s) 235, 382, 411, 452, 623, 1060, 1073
HpyF10VI GCNNNNNNNGC 1 cut(s) 944
HpyF3I CTNAG 6 cut(s) 339, 416, 557, 887, 972, 1002
HpySE526I ACGT 1 cut(s) 426
Hsp92I GRCGYC 1 cut(s) 61
Hsp92II CATG 4 cut(s) 243, 261, 632, 777
HspAI GCGC 1 cut(s) 61
KasI GGCGCC 1 cut(s) 60
Kpn2I TCCGGA 1 cut(s) 39
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 7 cut(s) 265, 571, 640, 813, 818, 843, 928
LmnI GCTCC 1 cut(s) 724
Lsp1109I GCAGC 1 cut(s) 596
LweI GCATC 1 cut(s) 439
MaeI CTAG 1 cut(s) 575
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 5 cut(s) 135, 349, 359, 466, 1034
MalI GATC 7 cut(s) 267, 573, 642, 815, 820, 845, 930
MboI GATC 7 cut(s) 265, 571, 640, 813, 818, 843, 928
MboII GAAGA 5 cut(s) 55, 159, 546, 665, 923
MhlI GDGCHC 3 cut(s) 21, 87, 185
MluCI AATT 5 cut(s) 124, 324, 596, 787, 962
Mly113I GGCGCC 1 cut(s) 61
MlyI GAGTC 1 cut(s) 367
MmeI TCCRAC 2 cut(s) 695, 871
Mph1103I ATGCAT 1 cut(s) 1062
MroI TCCGGA 1 cut(s) 39
MseI TTAA 5 cut(s) 132, 171, 504, 965, 1014
MslI CAYNNNNRTG 1 cut(s) 914
MspCI CTTAAG 1 cut(s) 131
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 2 cut(s) 104, 221
MvaI CCWGG 2 cut(s) 104, 221
MwoI GCNNNNNNNGC 1 cut(s) 944
NarI GGCGCC 1 cut(s) 61
NdeII GATC 7 cut(s) 265, 571, 640, 813, 818, 843, 928
NlaIII CATG 4 cut(s) 243, 261, 632, 777
NlaIV GGNNCC 5 cut(s) 18, 62, 108, 616, 726
NmuCI GTSAC 2 cut(s) 135, 359
NsiI ATGCAT 1 cut(s) 1062
NspV TTCGAA 1 cut(s) 145
PflMI CCANNNNNTGG 2 cut(s) 774, 879
PkrI GCNGC 2 cut(s) 586, 946
PleI GAGTC 1 cut(s) 366
PluTI GGCGCC 1 cut(s) 64
PpsI GAGTC 1 cut(s) 366
PshAI GACNNNNGTC 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 185
Psp6I CCWGG 2 cut(s) 102, 219
PspGI CCWGG 2 cut(s) 102, 219
PspN4I GGNNCC 5 cut(s) 18, 62, 108, 616, 726
PspPI GGNCC 2 cut(s) 615, 835
PsrI GAACNNNNNNTAC 2 cut(s) 282, 314
RsaI GTAC 2 cut(s) 300, 1011
RsaNI GTAC 2 cut(s) 299, 1010
RseI CAYNNNNRTG 1 cut(s) 914
SacI GAGCTC 1 cut(s) 185
SaqAI TTAA 5 cut(s) 132, 171, 504, 965, 1014
SatI GCNGC 2 cut(s) 585, 945
Sau3AI GATC 7 cut(s) 265, 571, 640, 813, 818, 843, 928
Sau96I GGNCC 2 cut(s) 615, 835
ScaI AGTACT 1 cut(s) 1011
SchI GAGTC 1 cut(s) 367
ScrFI CCNGG 2 cut(s) 104, 221
SduI GDGCHC 3 cut(s) 21, 87, 185
SfaNI GCATC 1 cut(s) 439
SfcI CTRYAG 1 cut(s) 119
SfoI GGCGCC 1 cut(s) 62
SfuI TTCGAA 1 cut(s) 145
SinI GGWCC 2 cut(s) 615, 835
SmiMI CAYNNNNRTG 1 cut(s) 914
SmlI CTYRAG 2 cut(s) 65, 131
SmoI CTYRAG 2 cut(s) 65, 131
Sse9I AATT 5 cut(s) 124, 324, 596, 787, 962
SsiI CCGC 2 cut(s) 900, 945
SspDI GGCGCC 1 cut(s) 60
SspI AATATT 3 cut(s) 463, 502, 517
SspMI CTAG 1 cut(s) 575
SstI GAGCTC 1 cut(s) 185
StyD4I CCNGG 2 cut(s) 102, 219
TaaI ACNGT 4 cut(s) 141, 178, 355, 852
TaiI ACGT 1 cut(s) 429
TaqI TCGA 1 cut(s) 145
TasI AATT 5 cut(s) 124, 324, 596, 787, 962
TatI WGTACW 2 cut(s) 298, 1009
TauI GCSGC 1 cut(s) 947
Tru1I TTAA 5 cut(s) 132, 171, 504, 965, 1014
Tru9I TTAA 5 cut(s) 132, 171, 504, 965, 1014
TscAI CASTG 4 cut(s) 183, 394, 424, 802
TseFI GTSAC 2 cut(s) 135, 359
TseI GCWGC 1 cut(s) 584
Tsp45I GTSAC 2 cut(s) 135, 359
TspDTI ATGAA 5 cut(s) 192, 246, 617, 680, 756
TspRI CASTG 4 cut(s) 183, 394, 424, 802
Van91I CCANNNNNTGG 2 cut(s) 774, 879
Vha464I CTTAAG 1 cut(s) 131
VpaK11BI GGWCC 2 cut(s) 615, 835
XapI RAATTY 3 cut(s) 124, 324, 596
XbaI TCTAGA 1 cut(s) 574
XcmI CCANNNNNNNNNTGG 2 cut(s) 538, 876
XspI CTAG 1 cut(s) 575
ZrmI AGTACT 1 cut(s) 1011
Zsp2I ATGCAT 1 cut(s) 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.