Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0001775

Physical Location & Seq

Genomic Coordinates Reverse (-)

70773509 .. 70779301

Gene Structure 5793 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG406400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGGCTCACGACAACGGTGACGGCGTTACTCACGACATGTTCTCGCTGCTCGCCTCCGGCGATCGGGACTTCCTCCTCCGTAACAACGGCGACCAGGTAAAGATCAATACTTTGACTGGTAAGATTTTGGGGTTGTATTTCTCTGGCTCATGGTGTGGTCCATGTCGTCGTTTCACCCCAAACTTGGTGGAGGTCTACCAGGACCTTGCTTCCAAAGGGGACTTTGAAGTTGTGTTTGTCTCTTCTGATAGAGATGAGGAGTCATTCAGTGGCTACTTCTCTGAAATGCCATGGCTTGCGGTTCCCTTTTCGGATTTGGAGACCCGAAAAGGCCTGAAAGACTTGTTCAAAGTCAGGGGAATTCCTCATCTTGTTGTTATTGATGCCAATGGGAAGGTTTCCACTGATAAAGGGACTGTAGTTGTGAGGGAGTATGGTGTGGAGGGATATCCGTTCACCGCGGAGAAGATTAGTCTCTTGGAAGAGCAAGAGGAGGCGGCTAAGAAGAATCAATCCTTGAGTTCTCTCTTGGTTTCTAATTCTCGGGATTACTTGGTTTCGAGTGATGGGAATAAGGTTGCTGAGCTTGCTGAGATTGAGGAGGCCAAACTCGAAGCACAGACTCTGGAGTCGCTTTTGGTGACTGAAGACTTAGACTTTGTGATAGAGACGAGCGGTGCCAAGGTACCGGTGTCTGAGCTTGTTGGGAAGCACATTCTGCTCTATTTCTCAGCTCATTGGTGTCCCCCATGTCGTTCATTCCTGCCTAAGCTCATAGCAGCATATCATGAGATCAAGGCTAAGGACAATGCATTTGAAATTATCTTTATCTCAAGTGATCGTGATCAATCCTCCTTCGATGACTTCTTTTCGAGCATGCCTTGGTTAGCCCTCCCATTTGGTGATCCAAGGAAGACGTTCCTGCAGCGCAAATTCAAAATCCAAGGCATTCCTGCTGTGGTAGCTATCAGCCCCACTGAGCATTTGAAGCATTTGGAAGAAAAGGTAGACCAAGAAGCAAAGGGGTGGCCTGAGAAAGTGAAGAGTGAGCTACATGTTGAACATGAACTTGCACGCACTCGTTGCCACGAATATGTGTGTAATGGGTGTAGGGAGTCCGGAAGTGGTTGGTCTTTTTCCTGTAAGAAGTGTGACTTTGATCTTCACCCACAATGTGCTCTGAAGAACAATGAAGCAACAAAGGATGATCCCGAGACCAAGGCAAAGGAGGGATATGTTTGTGATGGTGACGTGTGCCATTTGCTCTTTGCAGTAGTGATGGGTGAGACAGAGGTGCCCACGACAAGCATAGACATCCGGAAGAACGAGAAAAATGGGCGCCCCAAGGCATTGGATGTAGTGCTCTCTAAAGCTTTTGTCCAGGTTCCTCACAAGCTACAGAATTTTCTTAAGACACAGTTTAGAAGACTACCAAAAGCTGATGGAGTTAAAACAGTGAGCTCTGTCCCTAAAAAGGCTTCATCTTTGGAGGTTGACCTGGAAAAGCAGTTGCAAGCATGGAGAGAAAATCCTTCATGGGATGATCAAACACCACATATAAAGGTCAGTGTGCCAAAAGGCTCTCTCTGCAACCTCAGTGTAAACGTCAATGTTGGGTTGCCCCCAGATGCAGTATATAATATTGTAACTGACCCCGACAATAAGAGGGTTTTCAAGAATATTAAGGCAAGTGATAATGGTCTTTTTCTTCACCATGAGTGTTTTTCTCTGTACCTTTTCGTACTCCCCTTCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0003824 GO:0004791 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006935 GO:0008150 GO:0008152 GO:0009605 GO:0009636 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010035 GO:0010038 GO:0010183 GO:0010769 GO:0015036 GO:0016043 GO:0016049 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0022414 GO:0022603 GO:0022604 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0040008 GO:0040011 GO:0042221 GO:0042330 GO:0043900 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0045595 GO:0046686 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050918 GO:0051128 GO:0051239 GO:0051510 GO:0051704 GO:0051716 GO:0055114 GO:0060284 GO:0060560 GO:0065007 GO:0070887 GO:0071840 GO:0080092 GO:0097237 GO:0098754 GO:0098869 GO:1990748 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C1_2 Thioredoxin_8

Protein Analysis

585

Amino Acids

65.54

Weight (kDa)

5.66

Isoelectric Point (pI)

36.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AhpC-TSA	PF00578	25 - 134	6.3e-08	AhpC/TSA family
Redoxin	PF08534	27 - 135	2.4e-07	Redoxin
Thioredoxin_2	PF13098	39 - 132	5e-06	Thioredoxin-like domain
Thioredoxin_8	PF13905	40 - 133	3.4e-28	Thioredoxin-like
Redoxin	PF08534	223 - 286	9.4e-06	Redoxin
AhpC-TSA	PF00578	224 - 294	4.8e-08	AhpC/TSA family
Thioredoxin_8	PF13905	236 - 325	5e-26	Thioredoxin-like
Thioredoxin_2	PF13098	236 - 326	6.9e-06	Thioredoxin-like domain
C1_2	PF03107	352 - 393	1.3e-06	C1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0001775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70480 AT1G70480
fragaria_vesca	FvH4_4g34241 FvH4_4g34250 FvH4_4g34260 FvH4_4g34260 FvH4_4g34260 FvH4_4g34260
malus_domestica	MD13G1093300.v1.1 MD13G1093500.v1.1 MD16G1094500.v1.1 MD16G1094600.v1.1 MD16G1094700.v1.1
prunus_persica	Prupe.1G251900_v2.0.a1 Prupe.1G252000_v2.0.a1
pyrus_communis	pycom13g08130 pycom13g08140 pycom16g08030 pycom16g08040 pycom16g08050
rosa_chinensis	RchiOBHm_Chr4g0443711 RchiOBHm_Chr6g0250421
rosa_laevigata	RLG00000005898 RLG00000005899 RLG00000005900
rosa_multiflora	Rmu_co8463221.1_g000001 Rmu_sc0001426.1_g000010 Rmu_sc0001426.1_g000011 Rmu_sc0001426.1_g000013 Rmu_sc0008739.1_g000001
rosa_roxburghii	Rroxscaffold_5G00384250 Rroxscaffold_5G00384260 Rroxscaffold_5G00384270
rosa_rugosa	Rorug05G0496400 Rorug05G0496500
rosa_samantha	Rh4AG406400 Rh4AG406500 Rh4AG406600 Rh4BG417400 Rh4BG417500 Rh4BG417600 Rh4BG417700 Rh4CG431900 Rh4DG413000
rosa_wichuraiana	Rw4G034900 Rw4G034910 Rw4G034920

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	628
Acc65I	GGTACC	1 cut(s)	685
AccB1I	GGYRCC	4 cut(s)	677, 685, 1294, 1338
AccBSI	CCGCTC	1 cut(s)	675
AccI	GTMKAC	2 cut(s)	195, 1008
AccII	CGCG	1 cut(s)	461
AccIII	TCCGGA	2 cut(s)	1118, 1317
AciI	CCGC	5 cut(s)	299, 459, 461, 497, 675
AclWI	GGATC	2 cut(s)	899, 1202
AcsI	RAATTY	3 cut(s)	360, 932, 1402
AcuI	CTGAAG	2 cut(s)	666, 1202
AcyI	GRCGYC	1 cut(s)	1339
AdeI	CACNNNGTG	1 cut(s)	1175
AfaI	GTAC	3 cut(s)	687, 1733, 1743
AfiI	CCNNNNNNNGG	3 cut(s)	63, 184, 1474
AflII	CTTAAG	1 cut(s)	1409
AflIII	ACRYGT	3 cut(s)	36, 1054, 1251
AgeI	ACCGGT	1 cut(s)	688
AgsI	TTSAA	7 cut(s)	227, 349, 818, 937, 988, 1061, 1675
AjiI	CACGTC	1 cut(s)	1252
AjnI	CCWGG	4 cut(s)	93, 198, 1380, 1497
Alw21I	GWGCWC	3 cut(s)	1180, 1365, 1463
Alw26I	GTCTC	6 cut(s)	244, 314, 479, 662, 1208, 1280
AlwI	GGATC	2 cut(s)	899, 1202
AlwNI	CAGNNNCTG	1 cut(s)	625
Ama87I	CYCGRG	2 cut(s)	543, 1211
Aor13HI	TCCGGA	2 cut(s)	1118, 1317
AoxI	GGCC	3 cut(s)	331, 603, 1028
ApeKI	GCWGC	3 cut(s)	46, 779, 925
ApoI	RAATTY	3 cut(s)	360, 932, 1402
ArsI	GACNNNNNNTTYG	4 cut(s)	641, 673, 797, 829
AsiGI	ACCGGT	1 cut(s)	688
Asp700I	GAANNNNTTC	1 cut(s)	917
Asp718I	GGTACC	1 cut(s)	685
AspLEI	GCGC	2 cut(s)	930, 1341
AspS9I	GGNCC	2 cut(s)	158, 202
AsuHPI	GGTGA	9 cut(s)	29, 166, 448, 652, 914, 1157, 1259, 1295, 1703
AvaI	CYCGRG	2 cut(s)	543, 1211
AvaII	GGWCC	2 cut(s)	158, 202
BaeGI	GKGCMC	1 cut(s)	1299
BaeI	ACNNNNGTAYC	2 cut(s)	677, 710
BanI	GGYRCC	4 cut(s)	677, 685, 1294, 1338
BanII	GRGCYC	1 cut(s)	1463
BbsI	GAAGAC	3 cut(s)	654, 920, 1432
Bbv12I	GWGCWC	3 cut(s)	1180, 1365, 1463
BbvI	GCAGC	3 cut(s)	33, 791, 937
BccI	CCATC	4 cut(s)	560, 1238, 1273, 1436
BceAI	ACGGC	2 cut(s)	37, 103
BciT130I	CCWGG	4 cut(s)	95, 200, 1382, 1499
BclI	TGATCA	2 cut(s)	844, 1543
BcoDI	GTCTC	6 cut(s)	244, 314, 479, 662, 1208, 1280
BfmI	CTRYAG	3 cut(s)	417, 923, 1397
BfoI	RGCGCY	1 cut(s)	1342
BfrI	CTTAAG	1 cut(s)	1409
BisI	GCNGC	4 cut(s)	47, 498, 780, 926
BlpI	GCTNAGC	1 cut(s)	582
BlsI	GCNGC	4 cut(s)	48, 499, 781, 927
Bme1390I	CCNGG	4 cut(s)	95, 200, 1382, 1499
Bme18I	GGWCC	2 cut(s)	158, 202
BmeT110I	CYCGRG	2 cut(s)	543, 1211
BmgBI	CACGTC	1 cut(s)	1252
BmgT120I	GGNCC	2 cut(s)	158, 202
BmiI	GGNNCC	6 cut(s)	303, 679, 687, 1296, 1340, 1386
BmrFI	CCNGG	4 cut(s)	95, 200, 1382, 1499
BmsI	GCATC	2 cut(s)	373, 1618
BpiI	GAAGAC	3 cut(s)	654, 920, 1432
BpmI	CTGGAG	1 cut(s)	647
Bpu10I	CCTNAGC	2 cut(s)	768, 801
Bpu1102I	GCTNAGC	1 cut(s)	582
BpuEI	CTTGAG	2 cut(s)	538, 817
BsaBI	GATNNNNATC	1 cut(s)	843
BsaHI	GRCGYC	1 cut(s)	1339
BsaI	GGTCTC	2 cut(s)	314, 1208
BsaJI	CCNNGG	8 cut(s)	290, 459, 681, 881, 908, 943, 1218, 1344
BsaWI	WCCGGW	3 cut(s)	688, 1118, 1317
Bsc4I	CCNNNNNNNGG	3 cut(s)	63, 184, 1474
Bse118I	RCCGGY	1 cut(s)	688
Bse1I	ACTGG	1 cut(s)	121
Bse8I	GATNNNNATC	1 cut(s)	843
BseAI	TCCGGA	2 cut(s)	1118, 1317
BseBI	CCWGG	4 cut(s)	95, 200, 1382, 1499
BseDI	CCNNGG	8 cut(s)	290, 459, 681, 881, 908, 943, 1218, 1344
BseGI	GGATG	4 cut(s)	1210, 1314, 1360, 1546
BseJI	GATNNNNATC	1 cut(s)	843
BseLI	CCNNNNNNNGG	3 cut(s)	63, 184, 1474
BseMII	CTCAG	7 cut(s)	573, 582, 687, 744, 969, 1023, 1609
BseNI	ACTGG	1 cut(s)	121
BseRI	GAGGAG	4 cut(s)	65, 272, 506, 614
BseSI	GKGCMC	1 cut(s)	1299
BseXI	GCAGC	3 cut(s)	33, 791, 937
Bsh1236I	CGCG	1 cut(s)	461
Bsh1285I	CGRYCG	1 cut(s)	64
BshFI	GGCC	3 cut(s)	333, 605, 1030
BshNI	GGYRCC	4 cut(s)	677, 685, 1294, 1338
BshTI	ACCGGT	1 cut(s)	688
BsiEI	CGRYCG	1 cut(s)	64
BsiHKAI	GWGCWC	3 cut(s)	1180, 1365, 1463
BsiHKCI	CYCGRG	2 cut(s)	543, 1211
BsiSI	CCGG	4 cut(s)	57, 689, 1119, 1318
BslFI	GGGAC	5 cut(s)	80, 233, 427, 729, 1451
BslI	CCNNNNNNNGG	3 cut(s)	63, 184, 1474
BsmAI	GTCTC	6 cut(s)	244, 314, 479, 662, 1208, 1280
BsmBI	CGTCTC	1 cut(s)	662
BsmFI	GGGAC	5 cut(s)	80, 233, 427, 729, 1451
BsmI	GAATGC	1 cut(s)	948
BsnI	GGCC	3 cut(s)	333, 605, 1030
Bso31I	GGTCTC	2 cut(s)	314, 1208
BsoBI	CYCGRG	2 cut(s)	543, 1211
Bsp1286I	GDGCHC	4 cut(s)	1180, 1299, 1365, 1463
Bsp13I	TCCGGA	2 cut(s)	1118, 1317
Bsp143I	GATC	9 cut(s)	61, 102, 792, 838, 844, 904, 1159, 1207, 1543
Bsp1720I	GCTNAGC	1 cut(s)	582
Bsp19I	CCATGG	1 cut(s)	290
BspACI	CCGC	5 cut(s)	299, 459, 461, 497, 675
BspANI	GGCC	3 cut(s)	333, 605, 1030
BspCNI	CTCAG	7 cut(s)	574, 583, 688, 743, 970, 1024, 1608
BspEI	TCCGGA	2 cut(s)	1118, 1317
BspFNI	CGCG	1 cut(s)	461
BspHI	TCATGA	1 cut(s)	787
BspLI	GGNNCC	6 cut(s)	303, 679, 687, 1296, 1340, 1386
BspMAI	CTGCAG	1 cut(s)	927
BspPI	GGATC	2 cut(s)	899, 1202
BspQI	GCTCTTC	1 cut(s)	477
BspT107I	GGYRCC	4 cut(s)	677, 685, 1294, 1338
BspTI	CTTAAG	1 cut(s)	1409
BspTNI	GGTCTC	2 cut(s)	314, 1208
BsrBI	CCGCTC	1 cut(s)	675
BsrFI	RCCGGY	1 cut(s)	688
BsrI	ACTGG	1 cut(s)	121
BssAI	RCCGGY	1 cut(s)	688
BssECI	CCNNGG	8 cut(s)	290, 459, 681, 881, 908, 943, 1218, 1344
BssMI	GATC	9 cut(s)	61, 102, 792, 838, 844, 904, 1159, 1207, 1543
BssNI	GRCGYC	1 cut(s)	1339
BssT1I	CCWWGG	7 cut(s)	290, 681, 881, 908, 943, 1218, 1344
Bst2UI	CCWGG	4 cut(s)	95, 200, 1382, 1499
Bst4CI	ACNGT	4 cut(s)	17, 418, 1419, 1456
Bst6I	CTCTTC	3 cut(s)	247, 477, 1037
BstACI	GRCGYC	1 cut(s)	1339
BstAFI	CTTAAG	1 cut(s)	1409
BstAPI	GCANNNNNTGC	2 cut(s)	718, 1083
BstC8I	GCNNGC	6 cut(s)	51, 297, 588, 878, 1075, 1515
BstDSI	CCRYGG	2 cut(s)	290, 459
BstF5I	GGATG	4 cut(s)	1210, 1314, 1360, 1546
BstFNI	CGCG	1 cut(s)	461
BstH2I	RGCGCY	1 cut(s)	1342
BstHHI	GCGC	2 cut(s)	930, 1341
BstKTI	GATC	9 cut(s)	64, 105, 795, 841, 847, 907, 1162, 1210, 1546
BstMAI	GTCTC	6 cut(s)	244, 314, 479, 662, 1208, 1280
BstMBI	GATC	9 cut(s)	61, 102, 792, 838, 844, 904, 1159, 1207, 1543
BstMCI	CGRYCG	1 cut(s)	64
BstMWI	GCNNNNNNNGC	6 cut(s)	587, 718, 962, 988, 1083, 1587
BstNI	CCWGG	4 cut(s)	95, 200, 1382, 1499
BstNSI	RCATGY	3 cut(s)	40, 880, 1058
BstSCI	CCNGG	4 cut(s)	93, 198, 1380, 1497
BstSFI	CTRYAG	3 cut(s)	417, 923, 1397
BstSLI	GKGCMC	1 cut(s)	1299
BstUI	CGCG	1 cut(s)	461
BstV1I	GCAGC	3 cut(s)	33, 791, 937
BstV2I	GAAGAC	3 cut(s)	654, 920, 1432
BstXI	CCANNNNNNTGG	1 cut(s)	1351
BsuRI	GGCC	3 cut(s)	333, 605, 1030
BtgI	CCRYGG	2 cut(s)	290, 459
BtrI	CACGTC	1 cut(s)	1252
BtsCI	GGATG	4 cut(s)	1210, 1314, 1360, 1546
BtsIMutI	CAGTG	6 cut(s)	274, 402, 975, 1461, 1573, 1603
Cac8I	GCNNGC	6 cut(s)	51, 297, 588, 878, 1075, 1515
CaiI	CAGNNNCTG	1 cut(s)	625
CciI	TCATGA	1 cut(s)	787
CfoI	GCGC	2 cut(s)	930, 1341
Cfr10I	RCCGGY	1 cut(s)	688
Cfr13I	GGNCC	2 cut(s)	158, 202
Cfr42I	CCGCGG	1 cut(s)	462
CsiI	ACCWGGT	1 cut(s)	93
Csp6I	GTAC	3 cut(s)	686, 1732, 1742
CspAI	ACCGGT	1 cut(s)	688
CspCI	CAANNNNNGTGG	2 cut(s)	168, 203
CviQI	GTAC	3 cut(s)	686, 1732, 1742
DinI	GGCGCC	1 cut(s)	1340
DpnI	GATC	9 cut(s)	63, 104, 794, 840, 846, 906, 1161, 1209, 1545
DpnII	GATC	9 cut(s)	61, 102, 792, 838, 844, 904, 1159, 1207, 1543
DraIII	CACNNNGTG	1 cut(s)	1175
DrdI	GACNNNNNNGTC	1 cut(s)	628
DseDI	GACNNNNNNGTC	1 cut(s)	628
Eam1104I	CTCTTC	3 cut(s)	247, 477, 1037
EarI	CTCTTC	3 cut(s)	247, 477, 1037
Ecl136II	GAGCTC	1 cut(s)	1461
Eco130I	CCWWGG	7 cut(s)	290, 681, 881, 908, 943, 1218, 1344
Eco147I	AGGCCT	1 cut(s)	333
Eco24I	GRGCYC	1 cut(s)	1463
Eco31I	GGTCTC	2 cut(s)	314, 1208
Eco32I	GATATC	1 cut(s)	449
Eco47I	GGWCC	2 cut(s)	158, 202
Eco53kI	GAGCTC	1 cut(s)	1461
Eco57I	CTGAAG	2 cut(s)	666, 1202
Eco88I	CYCGRG	2 cut(s)	543, 1211
EcoICRI	GAGCTC	1 cut(s)	1461
EcoO109I	RGGNCCY	1 cut(s)	202
EcoRI	GAATTC	1 cut(s)	360
EcoRII	CCWGG	4 cut(s)	93, 198, 1380, 1497
EcoRV	GATATC	1 cut(s)	449
EcoT14I	CCWWGG	7 cut(s)	290, 681, 881, 908, 943, 1218, 1344
EcoT22I	ATGCAT	1 cut(s)	814
EcoT38I	GRGCYC	1 cut(s)	1463
EgeI	GGCGCC	1 cut(s)	1340
EheI	GGCGCC	1 cut(s)	1340
ErhI	CCWWGG	7 cut(s)	290, 681, 881, 908, 943, 1218, 1344
Esp3I	CGTCTC	1 cut(s)	662
FalI	AAGNNNNNCTT	2 cut(s)	1139, 1171
FaqI	GGGAC	5 cut(s)	80, 233, 427, 729, 1451
FbaI	TGATCA	2 cut(s)	844, 1543
FblI	GTMKAC	2 cut(s)	195, 1008
Fnu4HI	GCNGC	4 cut(s)	47, 498, 780, 926
FokI	GGATG	4 cut(s)	1217, 1301, 1367, 1553
FriOI	GRGCYC	1 cut(s)	1463
Fsp4HI	GCNGC	4 cut(s)	47, 498, 780, 926
GlaI	GCGC	2 cut(s)	929, 1340
GluI	GCNGC	4 cut(s)	47, 498, 780, 926
GsuI	CTGGAG	1 cut(s)	647
HaeII	RGCGCY	1 cut(s)	1342
HaeIII	GGCC	3 cut(s)	333, 605, 1030
HapII	CCGG	4 cut(s)	57, 689, 1119, 1318
HhaI	GCGC	2 cut(s)	930, 1341
Hin1I	GRCGYC	1 cut(s)	1339
Hin6I	GCGC	2 cut(s)	928, 1339
HinP1I	GCGC	2 cut(s)	928, 1339
HincII	GTYRAC	1 cut(s)	1495
HindII	GTYRAC	1 cut(s)	1495
HindIII	AAGCTT	1 cut(s)	1371
HinfI	GANTC	5 cut(s)	260, 508, 622, 629, 1115
HpaII	CCGG	4 cut(s)	57, 689, 1119, 1318
HphI	GGTGA	9 cut(s)	29, 166, 448, 652, 914, 1157, 1259, 1295, 1703
Hpy166II	GTNNAC	5 cut(s)	196, 456, 1009, 1495, 1603
Hpy188I	TCNGA	5 cut(s)	247, 283, 313, 697, 1182
Hpy8I	GTNNAC	5 cut(s)	196, 456, 1009, 1495, 1603
Hpy99I	CGWCG	1 cut(s)	171
HpyAV	CCTTC	3 cut(s)	388, 865, 1542
HpyCH4III	ACNGT	4 cut(s)	17, 418, 1419, 1456
HpyCH4IV	ACGT	3 cut(s)	917, 1251, 1605
HpyCH4V	TGCA	7 cut(s)	812, 925, 1073, 1271, 1513, 1590, 1631
HpyF10VI	GCNNNNNNNGC	6 cut(s)	587, 718, 962, 988, 1083, 1587
HpySE526I	ACGT	3 cut(s)	917, 1251, 1605
Hsp92I	GRCGYC	1 cut(s)	1339
HspAI	GCGC	2 cut(s)	928, 1339
KasI	GGCGCC	1 cut(s)	1338
Kpn2I	TCCGGA	2 cut(s)	1118, 1317
KpnI	GGTACC	1 cut(s)	689
Ksp22I	TGATCA	2 cut(s)	844, 1543
KspI	CCGCGG	1 cut(s)	462
Kzo9I	GATC	9 cut(s)	61, 102, 792, 838, 844, 904, 1159, 1207, 1543
LguI	GCTCTTC	1 cut(s)	477
Lsp1109I	GCAGC	3 cut(s)	33, 791, 937
LweI	GCATC	2 cut(s)	373, 1618
MabI	ACCWGGT	1 cut(s)	93
MaeII	ACGT	3 cut(s)	917, 1251, 1605
MaeIII	GTNAC	7 cut(s)	17, 25, 80, 640, 1151, 1247, 1645
MalI	GATC	9 cut(s)	63, 104, 794, 840, 846, 906, 1161, 1209, 1545
MbiI	CCGCTC	1 cut(s)	675
MboI	GATC	9 cut(s)	61, 102, 792, 838, 844, 904, 1159, 1207, 1543
MhlI	GDGCHC	4 cut(s)	1180, 1299, 1365, 1463
MluCI	AATT	5 cut(s)	360, 538, 819, 932, 1402
Mly113I	GGCGCC	1 cut(s)	1339
MlyI	GAGTC	4 cut(s)	269, 616, 638, 1124
Mph1103I	ATGCAT	1 cut(s)	814
MroI	TCCGGA	2 cut(s)	1118, 1317
MroXI	GAANNNNTTC	1 cut(s)	917
MseI	TTAA	3 cut(s)	1410, 1449, 1683
MslI	CAYNNNNRTG	1 cut(s)	1092
MspA1I	CMGCKG	1 cut(s)	461
MspCI	CTTAAG	1 cut(s)	1409
MspI	CCGG	4 cut(s)	57, 689, 1119, 1318
MspR9I	CCNGG	4 cut(s)	95, 200, 1382, 1499
Mva1269I	GAATGC	1 cut(s)	948
MvaI	CCWGG	4 cut(s)	95, 200, 1382, 1499
MvnI	CGCG	1 cut(s)	461
MwoI	GCNNNNNNNGC	6 cut(s)	587, 718, 962, 988, 1083, 1587
NarI	GGCGCC	1 cut(s)	1339
NcoI	CCATGG	1 cut(s)	290
NdeII	GATC	9 cut(s)	61, 102, 792, 838, 844, 904, 1159, 1207, 1543
NlaIV	GGNNCC	6 cut(s)	303, 679, 687, 1296, 1340, 1386
NmuCI	GTSAC	4 cut(s)	17, 640, 1151, 1247
NsiI	ATGCAT	1 cut(s)	814
NspI	RCATGY	3 cut(s)	40, 880, 1058
PaeI	GCATGC	1 cut(s)	880
PagI	TCATGA	1 cut(s)	787
PceI	AGGCCT	1 cut(s)	333
PciI	ACATGT	2 cut(s)	36, 1054
PciSI	GCTCTTC	1 cut(s)	477
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 57
PctI	GAATGC	1 cut(s)	948
PdmI	GAANNNNTTC	1 cut(s)	917
PfeI	GAWTC	1 cut(s)	508
PinAI	ACCGGT	1 cut(s)	688
PkrI	GCNGC	4 cut(s)	48, 499, 781, 927
Ple19I	CGATCG	1 cut(s)	64
PleI	GAGTC	4 cut(s)	268, 616, 637, 1123
PluTI	GGCGCC	1 cut(s)	1342
PpsI	GAGTC	4 cut(s)	268, 616, 637, 1123
PpuMI	RGGWCCY	1 cut(s)	202
PscI	ACATGT	2 cut(s)	36, 1054
Psp124BI	GAGCTC	1 cut(s)	1463
Psp5II	RGGWCCY	1 cut(s)	202
Psp6I	CCWGG	4 cut(s)	93, 198, 1380, 1497
PspGI	CCWGG	4 cut(s)	93, 198, 1380, 1497
PspN4I	GGNNCC	6 cut(s)	303, 679, 687, 1296, 1340, 1386
PspPI	GGNCC	2 cut(s)	158, 202
PspPPI	RGGWCCY	1 cut(s)	202
PstI	CTGCAG	1 cut(s)	927
PstNI	CAGNNNCTG	1 cut(s)	625
PvuI	CGATCG	1 cut(s)	64
RsaI	GTAC	3 cut(s)	687, 1733, 1743
RsaNI	GTAC	3 cut(s)	686, 1732, 1742
RseI	CAYNNNNRTG	1 cut(s)	1092
SacI	GAGCTC	1 cut(s)	1463
SacII	CCGCGG	1 cut(s)	462
SapI	GCTCTTC	1 cut(s)	477
SaqAI	TTAA	3 cut(s)	1410, 1449, 1683
SatI	GCNGC	4 cut(s)	47, 498, 780, 926
Sau3AI	GATC	9 cut(s)	61, 102, 792, 838, 844, 904, 1159, 1207, 1543
Sau96I	GGNCC	2 cut(s)	158, 202
SchI	GAGTC	4 cut(s)	269, 616, 638, 1124
ScrFI	CCNGG	4 cut(s)	95, 200, 1382, 1499
SduI	GDGCHC	4 cut(s)	1180, 1299, 1365, 1463
SexAI	ACCWGGT	1 cut(s)	93
SfaNI	GCATC	2 cut(s)	373, 1618
SfcI	CTRYAG	3 cut(s)	417, 923, 1397
SfoI	GGCGCC	1 cut(s)	1340
Sfr303I	CCGCGG	1 cut(s)	462
SgrBI	CCGCGG	1 cut(s)	462
SinI	GGWCC	2 cut(s)	158, 202
SmiMI	CAYNNNNRTG	1 cut(s)	1092
SmlI	CTYRAG	3 cut(s)	517, 832, 1409
SmoI	CTYRAG	3 cut(s)	517, 832, 1409
SphI	GCATGC	1 cut(s)	880
Sse9I	AATT	5 cut(s)	360, 538, 819, 932, 1402
SseBI	AGGCCT	1 cut(s)	333
SsiI	CCGC	5 cut(s)	299, 459, 461, 497, 675
SspDI	GGCGCC	1 cut(s)	1338
SspI	AATATT	2 cut(s)	1642, 1681
SstI	GAGCTC	1 cut(s)	1463
StuI	AGGCCT	1 cut(s)	333
StyD4I	CCNGG	4 cut(s)	93, 198, 1380, 1497
StyI	CCWWGG	7 cut(s)	290, 681, 881, 908, 943, 1218, 1344
TaaI	ACNGT	4 cut(s)	17, 418, 1419, 1456
TaiI	ACGT	3 cut(s)	920, 1254, 1608
TaqI	TCGA	4 cut(s)	560, 612, 858, 872
TasI	AATT	5 cut(s)	360, 538, 819, 932, 1402
TauI	GCSGC	1 cut(s)	500
TfiI	GAWTC	1 cut(s)	508
Tru1I	TTAA	3 cut(s)	1410, 1449, 1683
Tru9I	TTAA	3 cut(s)	1410, 1449, 1683
TscAI	CASTG	6 cut(s)	274, 409, 982, 1461, 1573, 1603
TseFI	GTSAC	4 cut(s)	17, 640, 1151, 1247
TseI	GCWGC	3 cut(s)	46, 779, 925
Tsp45I	GTSAC	4 cut(s)	17, 640, 1151, 1247
TspDTI	ATGAA	5 cut(s)	747, 1080, 1206, 1470, 1524
TspGWI	ACGGA	2 cut(s)	68, 441
TspRI	CASTG	6 cut(s)	274, 409, 982, 1461, 1573, 1603
Vha464I	CTTAAG	1 cut(s)	1409
VpaK11BI	GGWCC	2 cut(s)	158, 202
XapI	RAATTY	3 cut(s)	360, 932, 1402
XceI	RCATGY	3 cut(s)	40, 880, 1058
XmiI	GTMKAC	2 cut(s)	195, 1008
XmnI	GAANNNNTTC	1 cut(s)	917
Zsp2I	ATGCAT	1 cut(s)	814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.