AT1G77420

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001162
Physical Location & Seq
Forward (+)
29093499 .. 29095719
2221 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G77420.1

Sequence Viewer

Length: 1149 bp
ATGGATCGTGCTTTAACTCCGCGTTTTGATACTCCAGCTACTCTCTCCCCTTTCCGATACCGATATGGGAGCTCTCTGGCCATCACTCGACGATTCGCGCCGGCGATTGTTTGCGCGAAACGATCTCCGATCGATGGAGTGAGCGACGAGCTGAATCTGATTGCTTCACAGAACTTAGATCAAGCTCCCGCACGAAGACTAGCCCGTTCCGCTTTTGTTGACTTGCAGCTTCAGCTGGATCACTGCTTGTTCAAGAAAGCTCCGAGTGGGATCAGAACAGAGGAGTGGTATGAGAGAAATTCGAAAGGGGAAGATATTTTCTGCAAGAGTTGGTTGCCGAAATCCGGCGACGAAATCAAAGCTGCTGTATGTTTCTGTCATGGCTATGGAAGCACTTGCACTTTCTTCTTCGATGGTATAGCAAAGCAAATAGCAGGTTTTGGTTATGGGGTTTATGCTATAGACCATCCTGGTTTTGGTTTATCAGATGGTTTACATGGTCACATTCCAAGTTTTGATGACTTAGCAGACAATGCTATTGAACAGTTCACCAAAATGAAAGGGAGATCGGAATTGAGAAATTTGCCCCGGTTTTTGCTGGGACAGTCAATGGGAGGAGCAGTTGCCTTAAAAATTCATCTTAAAGAACCTCAAGCTTGGGATGGTCTTATCCTTGTCGCTCCAATGTGTAAGATTTCAGAAGATGTAAAACCTCCTCCACTAGTCTTGAAGACGTTAATCTTGATGTCGACACTATTTCCCAAAGCAAAGCTCTTTCCAAAGAGAGACTTGAGCGACTTCTTCTTCAGGGACCTGAGTAAAAGAAAATTGTGTGAGTACGATGTGATATGCTACGATGATCAAACACGTCTCAAAACTGCTGTTGAACTTCTAAATGCAACAAGAGACATTGAGATGCAAGTTGACAAGGTCTCGTTGCCACTGTTGATACTTCATGGAGACACTGATAAAGTCACAGATCCCACTGTGAGTAAATTCCTTCACAAACATGCAGTCAGCCAAGACAAGACTCTAAAACTCTATCCAGGTGGCTACCATTGCATTCTAGAAGGTGATACAGACGAAAATATTTTCACTGTAATCAATGACATCGTTGCTTGGCTCGATGCCCGCGTTGATCCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

382

Amino Acids

42.82

Weight (kDa)

7.12

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 118 - 356 7.9e-68 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 122 - 357 1.5e-13 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 125 - 357 1e-13 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 425
AccI GTMKAC 1 cut(s) 749
AccII CGCG 4 cut(s) 22, 98, 116, 1134
AciI CCGC 4 cut(s) 20, 189, 210, 1132
AclWI GGATC 5 cut(s) 12, 246, 278, 974, 1133
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 4 cut(s) 298, 580, 633, 995
AcuI CTGAAG 2 cut(s) 215, 790
AfaI GTAC 1 cut(s) 839
AfiI CCNNNNNNNGG 3 cut(s) 134, 344, 476
AflIII ACRYGT 1 cut(s) 866
AgsI TTSAA 4 cut(s) 253, 542, 730, 887
AhlI ACTAGT 1 cut(s) 721
AjiI CACGTC 1 cut(s) 869
AjnI CCWGG 2 cut(s) 469, 1045
Alw21I GWGCWC 1 cut(s) 74
Alw26I GTCTC 5 cut(s) 780, 875, 900, 937, 954
AlwI GGATC 5 cut(s) 12, 246, 278, 974, 1133
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 2 cut(s) 226, 362
ApoI RAATTY 4 cut(s) 298, 580, 633, 995
ArsI GACNNNNNNTTYG 2 cut(s) 999, 1031
AspLEI GCGC 2 cut(s) 100, 116
AspS9I GGNCC 1 cut(s) 811
AsuC2I CCSGG 1 cut(s) 589
AsuHPI GGTGA 2 cut(s) 541, 1085
AsuII TTCGAA 1 cut(s) 302
AvaII GGWCC 1 cut(s) 811
BalI TGGCCA 1 cut(s) 80
BanII GRGCYC 1 cut(s) 74
BbsI GAAGAC 2 cut(s) 202, 737
Bbv12I GWGCWC 1 cut(s) 74
BbvI GCAGC 2 cut(s) 238, 349
BccI CCATC 6 cut(s) 89, 128, 407, 474, 482, 656
BciT130I CCWGG 2 cut(s) 471, 1047
BclI TGATCA 1 cut(s) 859
BcnI CCSGG 1 cut(s) 589
BcoDI GTCTC 5 cut(s) 780, 875, 900, 937, 954
BcuI ACTAGT 1 cut(s) 721
BfaI CTAG 3 cut(s) 200, 722, 1067
BfmI CTRYAG 1 cut(s) 459
BfuAI ACCTGC 1 cut(s) 425
BisI GCNGC 2 cut(s) 227, 363
BlsI GCNGC 2 cut(s) 228, 364
Bme1390I CCNGG 3 cut(s) 471, 589, 1047
Bme18I GGWCC 1 cut(s) 811
BmgBI CACGTC 1 cut(s) 869
BmgT120I GGNCC 1 cut(s) 811
BmiI GGNNCC 1 cut(s) 812
BmrFI CCNGG 3 cut(s) 471, 589, 1047
BmsI GCATC 2 cut(s) 906, 1117
BpiI GAAGAC 2 cut(s) 202, 737
BpmI CTGGAG 1 cut(s) 18
Bpu14I TTCGAA 1 cut(s) 302
BpuEI CTTGAG 2 cut(s) 636, 811
BpuMI CCSGG 1 cut(s) 589
Bsa29I ATCGAT 1 cut(s) 132
BsaI GGTCTC 1 cut(s) 937
BsaJI CCNNGG 1 cut(s) 587
BsaXI ACNNNNNCTCC 2 cut(s) 606, 636
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 344, 476
Bse118I RCCGGY 1 cut(s) 100
Bse3DI GCAATG 1 cut(s) 1057
BseBI CCWGG 2 cut(s) 471, 1047
BseCI ATCGAT 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 587
BseGI GGATG 2 cut(s) 466, 667
BseLI CCNNNNNNNGG 3 cut(s) 134, 344, 476
BseMI GCAATG 1 cut(s) 1057
BseMII CTCAG 1 cut(s) 806
BseRI GAGGAG 3 cut(s) 296, 630, 705
BseXI GCAGC 2 cut(s) 238, 349
BseYI CCCAGC 1 cut(s) 598
Bsh1236I CGCG 4 cut(s) 22, 98, 116, 1134
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 1 cut(s) 80
BshVI ATCGAT 1 cut(s) 132
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 74
BsiSI CCGG 3 cut(s) 101, 345, 589
BslFI GGGAC 2 cut(s) 615, 824
BslI CCNNNNNNNGG 3 cut(s) 134, 344, 476
BsmAI GTCTC 5 cut(s) 780, 875, 900, 937, 954
BsmBI CGTCTC 1 cut(s) 875
BsmFI GGGAC 2 cut(s) 615, 824
BsmI GAATGC 1 cut(s) 1062
BsnI GGCC 1 cut(s) 80
Bso31I GGTCTC 1 cut(s) 937
Bsp119I TTCGAA 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 74
BspACI CCGC 4 cut(s) 20, 189, 210, 1132
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 1 cut(s) 807
BspDI ATCGAT 1 cut(s) 132
BspFNI CGCG 4 cut(s) 22, 98, 116, 1134
BspLI GGNNCC 1 cut(s) 812
BspMI ACCTGC 1 cut(s) 425
BspPI GGATC 5 cut(s) 12, 246, 278, 974, 1133
BspT104I TTCGAA 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 937
BsrDI GCAATG 1 cut(s) 1057
BsrFI RCCGGY 1 cut(s) 100
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 1 cut(s) 587
Bst2UI CCWGG 2 cut(s) 471, 1047
Bst4CI ACNGT 5 cut(s) 546, 606, 945, 988, 1099
BstAPI GCANNNNNTGC 1 cut(s) 533
BstBI TTCGAA 1 cut(s) 302
BstC8I GCNNGC 2 cut(s) 102, 1132
BstDEI CTNAG 3 cut(s) 175, 523, 815
BstF5I GGATG 2 cut(s) 466, 667
BstFNI CGCG 4 cut(s) 22, 98, 116, 1134
BstHHI GCGC 2 cut(s) 100, 116
BstMAI GTCTC 5 cut(s) 780, 875, 900, 937, 954
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 5 cut(s) 209, 232, 390, 533, 1059
BstNI CCWGG 2 cut(s) 471, 1047
BstNSI RCATGY 1 cut(s) 1013
BstSCI CCNGG 3 cut(s) 469, 587, 1045
BstSFI CTRYAG 1 cut(s) 459
BstUI CGCG 4 cut(s) 22, 98, 116, 1134
BstV1I GCAGC 2 cut(s) 238, 349
BstV2I GAAGAC 2 cut(s) 202, 737
BstX2I RGATCY 1 cut(s) 979
BstYI RGATCY 1 cut(s) 979
Bsu15I ATCGAT 1 cut(s) 132
BsuRI GGCC 1 cut(s) 80
BsuTUI ATCGAT 1 cut(s) 132
BtrI CACGTC 1 cut(s) 869
BtsCI GGATG 2 cut(s) 466, 667
BtsI GCAGTG 1 cut(s) 241
BtsIMutI CAGTG 5 cut(s) 241, 941, 963, 984, 1095
BveI ACCTGC 1 cut(s) 425
Cac8I GCNNGC 2 cut(s) 102, 1132
CfoI GCGC 2 cut(s) 100, 116
Cfr10I RCCGGY 1 cut(s) 100
Cfr13I GGNCC 1 cut(s) 811
ClaI ATCGAT 1 cut(s) 132
Csp6I GTAC 1 cut(s) 838
CspCI CAANNNNNGTGG 2 cut(s) 708, 743
CviAII CATG 4 cut(s) 380, 497, 956, 1010
CviQI GTAC 1 cut(s) 838
DdeI CTNAG 3 cut(s) 175, 523, 815
EaeI YGGCCR 1 cut(s) 78
Ecl136II GAGCTC 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 74
Eco31I GGTCTC 1 cut(s) 937
Eco47I GGWCC 1 cut(s) 811
Eco53kI GAGCTC 1 cut(s) 72
Eco57I CTGAAG 2 cut(s) 215, 790
EcoICRI GAGCTC 1 cut(s) 72
EcoO109I RGGNCCY 1 cut(s) 811
EcoRII CCWGG 2 cut(s) 469, 1045
EcoT38I GRGCYC 1 cut(s) 74
Esp3I CGTCTC 1 cut(s) 875
FaeI CATG 4 cut(s) 383, 500, 959, 1013
FalI AAGNNNNNCTT 2 cut(s) 773, 805
FaqI GGGAC 2 cut(s) 615, 824
FatI CATG 4 cut(s) 379, 496, 955, 1009
FauI CCCGC 2 cut(s) 196, 1139
FbaI TGATCA 1 cut(s) 859
FblI GTMKAC 1 cut(s) 749
Fnu4HI GCNGC 2 cut(s) 227, 363
FokI GGATG 2 cut(s) 453, 674
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 2 cut(s) 227, 363
FspBI CTAG 3 cut(s) 200, 722, 1067
GlaI GCGC 2 cut(s) 99, 115
GluI GCNGC 2 cut(s) 227, 363
GsaI CCCAGC 1 cut(s) 602
GsuI CTGGAG 1 cut(s) 18
HaeIII GGCC 1 cut(s) 80
HapII CCGG 3 cut(s) 101, 345, 589
HhaI GCGC 2 cut(s) 100, 116
Hin1II CATG 4 cut(s) 383, 500, 959, 1013
Hin6I GCGC 2 cut(s) 98, 114
HinP1I GCGC 2 cut(s) 98, 114
HincII GTYRAC 3 cut(s) 220, 750, 925
HindII GTYRAC 3 cut(s) 220, 750, 925
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 3 cut(s) 93, 154, 1030
HpaII CCGG 3 cut(s) 101, 345, 589
HphI GGTGA 2 cut(s) 541, 1085
Hpy166II GTNNAC 5 cut(s) 220, 494, 549, 750, 925
Hpy188I TCNGA 8 cut(s) 56, 129, 159, 264, 275, 487, 571, 700
Hpy188III TCNNGA 4 cut(s) 253, 727, 742, 1067
Hpy8I GTNNAC 5 cut(s) 220, 494, 549, 750, 925
Hpy99I CGWCG 3 cut(s) 93, 149, 353
HpyAV CCTTC 2 cut(s) 1010, 1064
HpyCH4III ACNGT 5 cut(s) 546, 606, 945, 988, 1099
HpyCH4IV ACGT 2 cut(s) 734, 868
HpyCH4V TGCA 7 cut(s) 226, 324, 399, 899, 919, 1013, 1062
HpyF10VI GCNNNNNNNGC 5 cut(s) 209, 232, 390, 533, 1059
HpyF3I CTNAG 3 cut(s) 175, 523, 815
HpySE526I ACGT 2 cut(s) 734, 868
Hsp92II CATG 4 cut(s) 383, 500, 959, 1013
HspAI GCGC 2 cut(s) 98, 114
KroI GCCGGC 1 cut(s) 100
KroNI GCCGGC 1 cut(s) 102
Ksp22I TGATCA 1 cut(s) 859
LmnI GCTCC 5 cut(s) 69, 190, 265, 617, 685
Lsp1109I GCAGC 2 cut(s) 238, 349
LweI GCATC 2 cut(s) 906, 1117
MaeI CTAG 3 cut(s) 200, 722, 1067
MaeII ACGT 2 cut(s) 734, 868
MaeIII GTNAC 2 cut(s) 500, 973
MboII GAAGA 8 cut(s) 207, 323, 397, 400, 713, 742, 793, 796
MflI RGATCY 1 cut(s) 979
MhlI GDGCHC 1 cut(s) 74
MlsI TGGCCA 1 cut(s) 80
MluCI AATT 6 cut(s) 298, 572, 580, 633, 827, 995
MluNI TGGCCA 1 cut(s) 80
MlyI GAGTC 1 cut(s) 1024
MnlI CCTC 5 cut(s) 274, 608, 660, 723, 726
Mox20I TGGCCA 1 cut(s) 80
MreI CGCCGGCG 1 cut(s) 100
MroNI GCCGGC 1 cut(s) 100
MscI TGGCCA 1 cut(s) 80
MseI TTAA 4 cut(s) 14, 629, 642, 737
MslI CAYNNNNRTG 4 cut(s) 384, 554, 914, 1008
Msp20I TGGCCA 1 cut(s) 80
MspA1I CMGCKG 1 cut(s) 235
MspI CCGG 3 cut(s) 101, 345, 589
MspR9I CCNGG 3 cut(s) 471, 589, 1047
Mva1269I GAATGC 1 cut(s) 1062
MvaI CCWGG 2 cut(s) 471, 1047
MvnI CGCG 4 cut(s) 22, 98, 116, 1134
MwoI GCNNNNNNNGC 5 cut(s) 209, 232, 390, 533, 1059
NaeI GCCGGC 1 cut(s) 102
NciI CCSGG 1 cut(s) 589
NgoMIV GCCGGC 1 cut(s) 100
NlaIII CATG 4 cut(s) 383, 500, 959, 1013
NlaIV GGNNCC 1 cut(s) 812
NmuCI GTSAC 2 cut(s) 500, 973
NspI RCATGY 1 cut(s) 1013
NspV TTCGAA 1 cut(s) 302
PcsI WCGNNNNNNNCGW 1 cut(s) 1131
PctI GAATGC 1 cut(s) 1062
PdiI GCCGGC 1 cut(s) 102
PfeI GAWTC 2 cut(s) 93, 154
PflFI GACNNNGTC 1 cut(s) 929
PkrI GCNGC 2 cut(s) 228, 364
Ple19I CGATCG 1 cut(s) 132
PleI GAGTC 1 cut(s) 1024
PpsI GAGTC 1 cut(s) 1024
PpuMI RGGWCCY 1 cut(s) 811
Psp124BI GAGCTC 1 cut(s) 74
Psp5II RGGWCCY 1 cut(s) 811
Psp6I CCWGG 2 cut(s) 469, 1045
PspFI CCCAGC 1 cut(s) 598
PspGI CCWGG 2 cut(s) 469, 1045
PspN4I GGNNCC 1 cut(s) 812
PspPI GGNCC 1 cut(s) 811
PspPPI RGGWCCY 1 cut(s) 811
PsuI RGATCY 1 cut(s) 979
PsyI GACNNNGTC 1 cut(s) 929
PvuI CGATCG 1 cut(s) 132
PvuII CAGCTG 1 cut(s) 235
RsaI GTAC 1 cut(s) 839
RsaNI GTAC 1 cut(s) 838
RseI CAYNNNNRTG 4 cut(s) 384, 554, 914, 1008
SacI GAGCTC 1 cut(s) 74
SalI GTCGAC 1 cut(s) 748
SaqAI TTAA 4 cut(s) 14, 629, 642, 737
SatI GCNGC 2 cut(s) 227, 363
Sau96I GGNCC 1 cut(s) 811
SchI GAGTC 1 cut(s) 1024
ScrFI CCNGG 3 cut(s) 471, 589, 1047
SduI GDGCHC 1 cut(s) 74
SfaNI GCATC 2 cut(s) 906, 1117
SfcI CTRYAG 1 cut(s) 459
SfuI TTCGAA 1 cut(s) 302
SgrAI CRCCGGYG 1 cut(s) 100
SinI GGWCC 1 cut(s) 811
SmiMI CAYNNNNRTG 4 cut(s) 384, 554, 914, 1008
SmlI CTYRAG 2 cut(s) 651, 790
SmoI CTYRAG 2 cut(s) 651, 790
SpeI ACTAGT 1 cut(s) 721
Sse9I AATT 6 cut(s) 298, 572, 580, 633, 827, 995
SsiI CCGC 4 cut(s) 20, 189, 210, 1132
SspI AATATT 1 cut(s) 1090
SspMI CTAG 3 cut(s) 200, 722, 1067
SstI GAGCTC 1 cut(s) 74
StyD4I CCNGG 3 cut(s) 469, 587, 1045
TaaI ACNGT 5 cut(s) 546, 606, 945, 988, 1099
TaiI ACGT 2 cut(s) 737, 871
TaqI TCGA 6 cut(s) 88, 132, 302, 411, 749, 1125
TasI AATT 6 cut(s) 298, 572, 580, 633, 827, 995
TfiI GAWTC 2 cut(s) 93, 154
Tru1I TTAA 4 cut(s) 14, 629, 642, 737
Tru9I TTAA 4 cut(s) 14, 629, 642, 737
TscAI CASTG 5 cut(s) 248, 948, 970, 991, 1102
TseFI GTSAC 2 cut(s) 500, 973
TseI GCWGC 2 cut(s) 226, 362
Tsp45I GTSAC 2 cut(s) 500, 973
TspDTI ATGAA 3 cut(s) 572, 626, 944
TspRI CASTG 5 cut(s) 248, 948, 970, 991, 1102
Tth111I GACNNNGTC 1 cut(s) 929
VpaK11BI GGWCC 1 cut(s) 811
XapI RAATTY 4 cut(s) 298, 580, 633, 995
XbaI TCTAGA 1 cut(s) 1066
XceI RCATGY 1 cut(s) 1013
XcmI CCANNNNNNNNNTGG 1 cut(s) 473
XmiI GTMKAC 1 cut(s) 749
XspI CTAG 3 cut(s) 200, 722, 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.