Rw4G029430

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001162
Physical Location & Seq
Forward (+)
56101164 .. 56103529
2366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029430.1

Sequence Viewer

Length: 1173 bp
ATGGAACGTTCTCGAGCTCTGAGATTCCAGCAACTGGAGCTTCCTTTCTGCCCCCAACAACTCCAAACCCAGAGGCCTACATTTTCAGTCGCCGCCAGAAGAACAAGTCAGATAATAGTTGCAGCGGCCAAGACCAAGAGAAGAAGGCCACCCATTGAAGGCGTTAGCGAGGAGCTCAATTTGATCGCCGATCAGAGCCTTGACTGTGCTCCGGCTCGGAGAAGAGTTCGATCCGCATTCGTTGAGCTGCAGCAGCAACTTGATCACTGCTTGTTTAAGTTGGCTCCAACTGGGATCAGAACAGAAGAGTGGTACGAAATAAATTCAAGGGGGCTAGGAATTTTCTGTAAAAGCTGGAAGCCCAGAGAAGGTGTTCCGATTAAAGGCGTATTGTGTTTCTGCCATGGTTATGGTGATACTTGCGTTTTCTTCTTTGAAGGTATTGCCAAGCAGATTGCGGCAGCTGGGTACGCTGTGTACGCCATGGACTACCCAGGCTTTGGCCTTTCAGAAGGATTGCATGGTTATATCCCGAGCTTTGATCAGCTAGCTGACGATGTTATTGAACAGTATACAAAGATTAAAGCAAAACCAGAGTTGAGAGGGTTGCCTCACTTCATATTGGGCCAGTCCATGGGTGGAGCTGTTACTCTCAAAGTTCACTTTAAGCAACCATATGCATGGGATGGCATAGTTCTTGTGGCTCCAATGTGTAAAATTGCAGAGGATGTAACACCTCCACCAGCGGCAGCGAAGCTATTAACTCTTATGTCCAATGTTGTCCCCAAAGCAAAGCTTGTCCCGCAGAAAGACTTGGCCGAGCTGGCCTTCAGAGATGAAAGGAAAAGGAAACTGGCTGTCTACAATGTGACATGCTACAATGGCCCCGTGCGATTGAAGACTGCTGTGGAACTACTAAACGCTACTAAGGAGATTGAGATGCAAGTGCATAAGGTTTCATCTCCACTACTAGTTCTTCATGGAGCTGCAGATAAGGTGACTGATCCCTTGGTGAGCCAGTTTCTTTATGAGAGGGCCTCAAGCAAAGACAAAACTCTAAAACTCTACCCAGATGGCTTTCATTGCATTCTTGAAGGAGAACCTGATGATAGAATCTTTTCAGTCCTTGAAGATATTATCACTTGGCTTGATCACCGGTGTTTGCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

390

Amino Acids

43.79

Weight (kDa)

8.75

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 127 - 368 1.2e-66 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 130 - 367 1.7e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 133 - 371 6.2e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 34, 500, 634
AccI GTMKAC 2 cut(s) 572, 861
AciI CCGC 6 cut(s) 93, 125, 234, 458, 746, 803
AclI AACGTT 1 cut(s) 7
AclWI GGATC 3 cut(s) 225, 302, 998
AcoI YGGCCR 2 cut(s) 126, 816
AcsI RAATTY 2 cut(s) 322, 339
AcuI CTGAAG 1 cut(s) 814
AfaI GTAC 3 cut(s) 314, 470, 479
AfiI CCNNNNNNNGG 6 cut(s) 34, 158, 368, 383, 500, 634
AgeI ACCGGT 1 cut(s) 1155
AgsI TTSAA 7 cut(s) 158, 327, 437, 566, 898, 1094, 1130
AhlI ACTAGT 1 cut(s) 970
AjnI CCWGG 1 cut(s) 493
Alw21I GWGCWC 3 cut(s) 19, 177, 211
AlwI GGATC 3 cut(s) 225, 302, 998
AlwNI CAGNNNCTG 1 cut(s) 34
Ama87I CYCGRG 2 cut(s) 12, 532
AoxI GGCC 9 cut(s) 74, 126, 146, 502, 625, 816, 825, 883, 1035
ApeKI GCWGC 7 cut(s) 122, 247, 250, 253, 461, 749, 986
ApoI RAATTY 2 cut(s) 322, 339
AsiGI ACCGGT 1 cut(s) 1155
Asp700I GAANNNNTTC 2 cut(s) 372, 1117
AspS9I GGNCC 3 cut(s) 625, 884, 1035
AsuHPI GGTGA 4 cut(s) 425, 1009, 1024, 1145
AsuNHI GCTAGC 1 cut(s) 547
AvaI CYCGRG 2 cut(s) 12, 532
BaeI ACNNNNGTAYC 2 cut(s) 408, 441
BanII GRGCYC 2 cut(s) 19, 177
BbsI GAAGAC 1 cut(s) 905
Bbv12I GWGCWC 3 cut(s) 19, 177, 211
BbvI GCAGC 7 cut(s) 134, 234, 262, 265, 473, 761, 973
BccI CCATC 2 cut(s) 680, 1067
BciT130I CCWGG 1 cut(s) 495
BclI TGATCA 3 cut(s) 262, 541, 1150
BcuI ACTAGT 1 cut(s) 970
BfaI CTAG 3 cut(s) 335, 548, 971
BfmI CTRYAG 2 cut(s) 248, 987
BglI GCCNNNNNGGC 1 cut(s) 824
Bme1390I CCNGG 1 cut(s) 495
BmeT110I CYCGRG 2 cut(s) 12, 532
BmgT120I GGNCC 3 cut(s) 625, 884, 1035
BmiI GGNNCC 3 cut(s) 285, 705, 886
BmrFI CCNGG 1 cut(s) 495
BmrI ACTGGG 1 cut(s) 300
BmsI GCATC 1 cut(s) 930
BmtI GCTAGC 1 cut(s) 551
BmuI ACTGGG 1 cut(s) 300
BpiI GAAGAC 1 cut(s) 905
BplI GAGNNNNNCTC 2 cut(s) 1022, 1054
BpmI CTGGAG 1 cut(s) 56
BpuEI CTTGAG 2 cut(s) 1024, 1151
BsaJI CCNNGG 5 cut(s) 403, 483, 493, 633, 1008
BsaWI WCCGGW 1 cut(s) 1155
Bsc4I CCNNNNNNNGG 6 cut(s) 34, 158, 368, 383, 500, 634
Bse118I RCCGGY 1 cut(s) 1155
Bse1I ACTGG 5 cut(s) 39, 295, 628, 858, 1018
Bse3DI GCAATG 1 cut(s) 1081
BseBI CCWGG 1 cut(s) 495
BseDI CCNNGG 5 cut(s) 403, 483, 493, 633, 1008
BseGI GGATG 2 cut(s) 691, 733
BseLI CCNNNNNNNGG 6 cut(s) 34, 158, 368, 383, 500, 634
BseMI GCAATG 1 cut(s) 1081
BseMII CTCAG 1 cut(s) 11
BseNI ACTGG 5 cut(s) 39, 295, 628, 858, 1018
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 7 cut(s) 134, 234, 262, 265, 473, 761, 973
BseYI CCCAGC 1 cut(s) 464
BshFI GGCC 9 cut(s) 76, 128, 148, 504, 627, 818, 827, 885, 1037
BshTI ACCGGT 1 cut(s) 1155
BsiHKAI GWGCWC 3 cut(s) 19, 177, 211
BsiHKCI CYCGRG 2 cut(s) 12, 532
BsiSI CCGG 2 cut(s) 212, 1156
BslFI GGGAC 2 cut(s) 767, 785
BslI CCNNNNNNNGG 6 cut(s) 34, 158, 368, 383, 500, 634
BsmFI GGGAC 2 cut(s) 767, 785
BsmI GAATGC 2 cut(s) 236, 1086
BsnI GGCC 9 cut(s) 76, 128, 148, 504, 627, 818, 827, 885, 1037
BsoBI CYCGRG 2 cut(s) 12, 532
Bsp1286I GDGCHC 3 cut(s) 19, 177, 211
Bsp143I GATC 8 cut(s) 183, 190, 230, 262, 294, 541, 1003, 1150
Bsp19I CCATGG 3 cut(s) 403, 483, 633
BspACI CCGC 6 cut(s) 93, 125, 234, 458, 746, 803
BspANI GGCC 9 cut(s) 76, 128, 148, 504, 627, 818, 827, 885, 1037
BspCNI CTCAG 1 cut(s) 12
BspLI GGNNCC 3 cut(s) 285, 705, 886
BspMAI CTGCAG 2 cut(s) 252, 991
BspOI GCTAGC 1 cut(s) 551
BspPI GGATC 3 cut(s) 225, 302, 998
BsrDI GCAATG 1 cut(s) 1081
BsrFI RCCGGY 1 cut(s) 1155
BsrI ACTGG 5 cut(s) 39, 295, 628, 858, 1018
BssAI RCCGGY 1 cut(s) 1155
BssECI CCNNGG 5 cut(s) 403, 483, 493, 633, 1008
BssMI GATC 8 cut(s) 183, 190, 230, 262, 294, 541, 1003, 1150
BssNAI GTATAC 1 cut(s) 573
BssT1I CCWWGG 4 cut(s) 403, 483, 633, 1008
Bst1107I GTATAC 1 cut(s) 573
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 2 cut(s) 206, 570
Bst6I CTCTTC 2 cut(s) 217, 300
BstC8I GCNNGC 2 cut(s) 549, 825
BstDEI CTNAG 2 cut(s) 20, 927
BstDSI CCRYGG 3 cut(s) 403, 483, 633
BstF5I GGATG 2 cut(s) 691, 733
BstKTI GATC 8 cut(s) 186, 193, 233, 265, 297, 544, 1006, 1153
BstMBI GATC 8 cut(s) 183, 190, 230, 262, 294, 541, 1003, 1150
BstMWI GCNNNNNNNGC 8 cut(s) 37, 253, 470, 479, 802, 824, 882, 1083
BstNI CCWGG 1 cut(s) 495
BstNSI RCATGY 1 cut(s) 876
BstSCI CCNGG 1 cut(s) 493
BstSFI CTRYAG 2 cut(s) 248, 987
BstV1I GCAGC 7 cut(s) 134, 234, 262, 265, 473, 761, 973
BstV2I GAAGAC 1 cut(s) 905
BstXI CCANNNNNNTGG 2 cut(s) 410, 681
BstZ17I GTATAC 1 cut(s) 573
BsuRI GGCC 9 cut(s) 76, 128, 148, 504, 627, 818, 827, 885, 1037
BtgI CCRYGG 3 cut(s) 403, 483, 633
BtsCI GGATG 2 cut(s) 691, 733
BtsI GCAGTG 1 cut(s) 265
BtsIMutI CAGTG 1 cut(s) 265
Cac8I GCNNGC 2 cut(s) 549, 825
CaiI CAGNNNCTG 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 1155
Cfr13I GGNCC 3 cut(s) 625, 884, 1035
Csp6I GTAC 3 cut(s) 313, 469, 478
CspAI ACCGGT 1 cut(s) 1155
CviAII CATG 7 cut(s) 404, 484, 521, 634, 681, 873, 980
CviQI GTAC 3 cut(s) 313, 469, 478
DdeI CTNAG 2 cut(s) 20, 927
DpnI GATC 8 cut(s) 185, 192, 232, 264, 296, 543, 1005, 1152
DpnII GATC 8 cut(s) 183, 190, 230, 262, 294, 541, 1003, 1150
EaeI YGGCCR 2 cut(s) 126, 816
Eam1104I CTCTTC 2 cut(s) 217, 300
EarI CTCTTC 2 cut(s) 217, 300
Ecl136II GAGCTC 2 cut(s) 17, 175
Eco130I CCWWGG 4 cut(s) 403, 483, 633, 1008
Eco147I AGGCCT 1 cut(s) 76
Eco24I GRGCYC 2 cut(s) 19, 177
Eco53kI GAGCTC 2 cut(s) 17, 175
Eco57I CTGAAG 1 cut(s) 814
Eco88I CYCGRG 2 cut(s) 12, 532
EcoICRI GAGCTC 2 cut(s) 17, 175
EcoO109I RGGNCCY 1 cut(s) 1035
EcoRII CCWGG 1 cut(s) 493
EcoT14I CCWWGG 4 cut(s) 403, 483, 633, 1008
EcoT22I ATGCAT 1 cut(s) 682
EcoT38I GRGCYC 2 cut(s) 19, 177
ErhI CCWWGG 4 cut(s) 403, 483, 633, 1008
FaeI CATG 7 cut(s) 407, 487, 524, 637, 684, 876, 983
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FaqI GGGAC 2 cut(s) 767, 785
FatI CATG 7 cut(s) 403, 483, 520, 633, 680, 872, 979
FauI CCCGC 1 cut(s) 810
FauNDI CATATG 1 cut(s) 676
FbaI TGATCA 3 cut(s) 262, 541, 1150
FblI GTMKAC 2 cut(s) 572, 861
FokI GGATG 2 cut(s) 698, 740
FriOI GRGCYC 2 cut(s) 19, 177
FspBI CTAG 3 cut(s) 335, 548, 971
GsaI CCCAGC 1 cut(s) 468
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 9 cut(s) 76, 128, 148, 504, 627, 818, 827, 885, 1037
HapII CCGG 2 cut(s) 212, 1156
Hin1II CATG 7 cut(s) 407, 487, 524, 637, 684, 876, 983
HindIII AAGCTT 1 cut(s) 794
HinfI GANTC 2 cut(s) 24, 1113
HpaII CCGG 2 cut(s) 212, 1156
HphI GGTGA 4 cut(s) 425, 1009, 1024, 1145
Hpy166II GTNNAC 4 cut(s) 478, 573, 661, 862
Hpy188I TCNGA 8 cut(s) 21, 111, 195, 219, 299, 378, 511, 833
Hpy188III TCNNGA 3 cut(s) 12, 532, 1091
Hpy8I GTNNAC 4 cut(s) 478, 573, 661, 862
HpyAV CCTTC 7 cut(s) 138, 152, 362, 431, 506, 838, 1088
HpyCH4III ACNGT 2 cut(s) 206, 570
HpyCH4IV ACGT 1 cut(s) 7
HpyCH4V TGCA 9 cut(s) 122, 250, 520, 680, 722, 943, 949, 989, 1086
HpyF10VI GCNNNNNNNGC 8 cut(s) 37, 253, 470, 479, 802, 824, 882, 1083
HpyF3I CTNAG 2 cut(s) 20, 927
HpySE526I ACGT 1 cut(s) 7
Hsp92II CATG 7 cut(s) 407, 487, 524, 637, 684, 876, 983
Ksp22I TGATCA 3 cut(s) 262, 541, 1150
Kzo9I GATC 8 cut(s) 183, 190, 230, 262, 294, 541, 1003, 1150
LmnI GCTCC 7 cut(s) 37, 172, 214, 289, 641, 709, 983
Lsp1109I GCAGC 7 cut(s) 134, 234, 262, 265, 473, 761, 973
LweI GCATC 1 cut(s) 930
MaeI CTAG 3 cut(s) 335, 548, 971
MaeII ACGT 1 cut(s) 7
MaeIII GTNAC 4 cut(s) 646, 730, 868, 997
MalI GATC 8 cut(s) 185, 192, 232, 264, 296, 543, 1005, 1152
MboI GATC 8 cut(s) 183, 190, 230, 262, 294, 541, 1003, 1150
MboII GAAGA 8 cut(s) 111, 153, 234, 317, 421, 910, 968, 1142
MhlI GDGCHC 3 cut(s) 19, 177, 211
MluCI AATT 4 cut(s) 178, 322, 339, 717
MmeI TCCRAC 1 cut(s) 311
MnlI CCTC 8 cut(s) 66, 163, 596, 621, 718, 747, 1026, 1048
Mph1103I ATGCAT 1 cut(s) 682
MroXI GAANNNNTTC 2 cut(s) 372, 1117
MseI TTAA 5 cut(s) 276, 381, 582, 666, 761
MslI CAYNNNNRTG 2 cut(s) 408, 679
MspA1I CMGCKG 3 cut(s) 125, 464, 746
MspI CCGG 2 cut(s) 212, 1156
MspR9I CCNGG 1 cut(s) 495
Mva1269I GAATGC 2 cut(s) 236, 1086
MvaI CCWGG 1 cut(s) 495
MwoI GCNNNNNNNGC 8 cut(s) 37, 253, 470, 479, 802, 824, 882, 1083
NcoI CCATGG 3 cut(s) 403, 483, 633
NdeI CATATG 1 cut(s) 676
NdeII GATC 8 cut(s) 183, 190, 230, 262, 294, 541, 1003, 1150
NheI GCTAGC 1 cut(s) 547
NlaIII CATG 7 cut(s) 407, 487, 524, 637, 684, 876, 983
NlaIV GGNNCC 3 cut(s) 285, 705, 886
NmeAIII GCCGAG 1 cut(s) 844
NmuCI GTSAC 2 cut(s) 868, 997
NsiI ATGCAT 1 cut(s) 682
NspI RCATGY 1 cut(s) 876
PaeR7I CTCGAG 1 cut(s) 12
PceI AGGCCT 1 cut(s) 76
PctI GAATGC 2 cut(s) 236, 1086
PdmI GAANNNNTTC 2 cut(s) 372, 1117
PfeI GAWTC 2 cut(s) 24, 1113
PflMI CCANNNNNTGG 3 cut(s) 34, 500, 634
PinAI ACCGGT 1 cut(s) 1155
Psp124BI GAGCTC 2 cut(s) 19, 177
Psp1406I AACGTT 1 cut(s) 7
Psp6I CCWGG 1 cut(s) 493
PspFI CCCAGC 1 cut(s) 464
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 3 cut(s) 285, 705, 886
PspPI GGNCC 3 cut(s) 625, 884, 1035
PstI CTGCAG 2 cut(s) 252, 991
PstNI CAGNNNCTG 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 3 cut(s) 314, 470, 479
RsaNI GTAC 3 cut(s) 313, 469, 478
RseI CAYNNNNRTG 2 cut(s) 408, 679
SacI GAGCTC 2 cut(s) 19, 177
SaqAI TTAA 5 cut(s) 276, 381, 582, 666, 761
Sau3AI GATC 8 cut(s) 183, 190, 230, 262, 294, 541, 1003, 1150
Sau96I GGNCC 3 cut(s) 625, 884, 1035
ScrFI CCNGG 1 cut(s) 495
SduI GDGCHC 3 cut(s) 19, 177, 211
SfaNI GCATC 1 cut(s) 930
SfcI CTRYAG 2 cut(s) 248, 987
SfiI GGCCNNNNNGGCC 1 cut(s) 824
Sfr274I CTCGAG 1 cut(s) 12
SgrAI CRCCGGYG 1 cut(s) 1155
SlaI CTCGAG 1 cut(s) 12
SmiMI CAYNNNNRTG 2 cut(s) 408, 679
SmlI CTYRAG 3 cut(s) 12, 1039, 1166
SmoI CTYRAG 3 cut(s) 12, 1039, 1166
SpeI ACTAGT 1 cut(s) 970
Sse9I AATT 4 cut(s) 178, 322, 339, 717
SseBI AGGCCT 1 cut(s) 76
SsiI CCGC 6 cut(s) 93, 125, 234, 458, 746, 803
SspMI CTAG 3 cut(s) 335, 548, 971
SstI GAGCTC 2 cut(s) 19, 177
StuI AGGCCT 1 cut(s) 76
StyD4I CCNGG 1 cut(s) 493
StyI CCWWGG 4 cut(s) 403, 483, 633, 1008
TaaI ACNGT 2 cut(s) 206, 570
TaiI ACGT 1 cut(s) 10
TaqI TCGA 2 cut(s) 13, 229
TasI AATT 4 cut(s) 178, 322, 339, 717
TauI GCSGC 4 cut(s) 95, 128, 461, 749
TfiI GAWTC 2 cut(s) 24, 1113
Tru1I TTAA 5 cut(s) 276, 381, 582, 666, 761
Tru9I TTAA 5 cut(s) 276, 381, 582, 666, 761
TscAI CASTG 1 cut(s) 272
TseFI GTSAC 2 cut(s) 868, 997
TseI GCWGC 7 cut(s) 122, 247, 250, 253, 461, 749, 986
Tsp45I GTSAC 2 cut(s) 868, 997
TspDTI ATGAA 5 cut(s) 607, 852, 948, 968, 1070
TspRI CASTG 1 cut(s) 272
Van91I CCANNNNNTGG 3 cut(s) 34, 500, 634
XapI RAATTY 2 cut(s) 322, 339
XceI RCATGY 1 cut(s) 876
XcmI CCANNNNNNNNNTGG 1 cut(s) 635
XhoI CTCGAG 1 cut(s) 12
XmiI GTMKAC 2 cut(s) 572, 861
XmnI GAANNNNTTC 2 cut(s) 372, 1117
XspI CTAG 3 cut(s) 335, 548, 971
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.