RchiOBHm_Chr4g0435041

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001162
Physical Location & Seq
Forward (+)
58666937 .. 58669199
2263 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40348

Sequence Viewer

Length: 1203 bp
ATGACAGCTATGAACCTTTCCCTGAGTTTCCGATCACCAGAGGCGTCTCTGTTTCACCGGAGATATAGCTCTCCGATCAAACACCTGCAAAAACAGCAAGTACCCAATTTACTCTCGTTCCCCAAACTACAATTTCCAGTGCCCAAAACTCAGTTCAGGGTCACGGCTCGGAAAGGGTCAGCTATAGAAGGCTTAAGCCAAGAGATGAACACCATCGCCTCGCAGAACCTGGATCACGCGCCTGCTCGGAGACGAGTTCGCTCGGCATTCATCGACTTGCATAAACAACTTGACCATTGCTTGTTCAAGATGGATCATGCTGGCATCAGAACAGAGGAGTGGTATGGAAGGAATTCAAGGGGTATGGAAATTTTCTGCAAAAGCTGGTTGCCGAAACCGGGTGATCAAATCAAAGCTGGCTTATGTTTTTGCCATGGGTACGGTAGTACTTGCACATTCTTCTTTGAAGGTATTGCTAGGAGAATTGCTCAGTCTGGGTACGCTGTATATGCAGTAGACTATCCAGGTTTTGGTCTTTCTGAAGGGTTGCATGGTTTCATCCCAGACTTTGATGAGTTGGTCGATGATGTTATTGAACAATTTACTAGTATCAAAGGAAGATCTGAAGTGAAAGGTTTGCCCTTTTTTATAATGGGGGAGTCGATGGGTGGAGCTGTCACTCTAAAGATTCATCTAAAGGAACCGCATGAATGGGATGGAGTGATCCTTGTATCTCCAATGTGTAAAATTGCAGATGATGTGATGCCTCCAGCAATAGTGCTGAAGCTATTAGCCTTCATGTCTGAAGTTTTGCCCGAGGCAAAACTCTTCCCACAGAAAGAGATAGCTCATCTAAGCTACAGAGAGCTCGGAAAGAGAAAAACGGCTGGTTACAATGTGATTTCTTATAAGGACCAAATGCGATCAAGAACAGCTGTAGAACTTCTGAAGGCCACTAGCGATATTGAAATGCAGTTGGATAAGGTTTCATCCCCATTGCTAATTCTTCATGGAGAAGCAGATAAGGTGACAGACCCTTTGGTGAGCCAGTTGCTGTATGAAAAGTCCTCTAGCAAGGATAAGACTTTGAAGCTCTATCAAGATGGTTATCACTGCATCCTTCAAGGGGAGCCTGATGACACAATTTTCACCGTCCTTGATGACATTGTTACTTGGCTTGATTCCCGGTGCTTCCGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

400

Amino Acids

45.22

Weight (kDa)

7.63

Isoelectric Point (pI)

48.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 136 - 378 6.1e-61 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 141 - 374 8.4e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 143 - 380 1.1e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 650, 909
AarI CACCTGC 1 cut(s) 93
Acc36I ACCTGC 1 cut(s) 93
AccB7I CCANNNNNTGG 1 cut(s) 530
AccI GTMKAC 1 cut(s) 516
AccII CGCG 1 cut(s) 239
AciI CCGC 1 cut(s) 704
AclWI GGATC 3 cut(s) 240, 321, 718
AcsI RAATTY 2 cut(s) 352, 369
AcuI CTGAAG 5 cut(s) 561, 645, 803, 825, 968
AcyI GRCGYC 1 cut(s) 44
AfaI GTAC 4 cut(s) 102, 440, 448, 500
AfiI CCNNNNNNNGG 3 cut(s) 398, 530, 1126
AflII CTTAAG 1 cut(s) 193
AgsI TTSAA 7 cut(s) 307, 357, 467, 596, 968, 1090, 1124
AhlI ACTAGT 1 cut(s) 605
AjnI CCWGG 2 cut(s) 228, 523
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
Alw21I GWGCWC 1 cut(s) 870
Alw26I GTCTC 2 cut(s) 51, 244
AlwI GGATC 3 cut(s) 240, 321, 718
AlwNI CAGNNNCTG 2 cut(s) 229, 1054
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 1 cut(s) 951
ApoI RAATTY 2 cut(s) 352, 369
ArsI GACNNNNNNTTYG 2 cut(s) 1021, 1053
Asp700I GAANNNNTTC 1 cut(s) 352
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 1 cut(s) 913
AsuC2I CCSGG 2 cut(s) 399, 1186
AsuHPI GGTGA 6 cut(s) 27, 47, 413, 1039, 1054, 1141
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 1 cut(s) 913
BaeGI GKGCMC 1 cut(s) 144
BaeI ACNNNNGTAYC 2 cut(s) 430, 463
BanII GRGCYC 1 cut(s) 870
Bbv12I GWGCWC 1 cut(s) 870
BccI CCATC 5 cut(s) 221, 304, 658, 710, 1097
BceAI ACGGC 2 cut(s) 180, 900
BciT130I CCWGG 2 cut(s) 230, 525
BclI TGATCA 1 cut(s) 403
BcnI CCSGG 2 cut(s) 399, 1186
BcoDI GTCTC 2 cut(s) 51, 244
BcuI ACTAGT 1 cut(s) 605
BfaI CTAG 4 cut(s) 477, 606, 957, 1071
BfmI CTRYAG 3 cut(s) 183, 859, 936
BfrI CTTAAG 1 cut(s) 193
BfuAI ACCTGC 1 cut(s) 93
BglII AGATCT 1 cut(s) 620
BmcAI AGTACT 1 cut(s) 448
Bme1390I CCNGG 4 cut(s) 230, 399, 525, 1186
Bme18I GGWCC 1 cut(s) 913
BmeT110I CYCGRG 1 cut(s) 815
BmgT120I GGNCC 1 cut(s) 913
BmiI GGNNCC 2 cut(s) 702, 1131
BmrFI CCNGG 4 cut(s) 230, 399, 525, 1186
BmsI GCATC 3 cut(s) 333, 753, 1125
BplI GAGNNNNNCTC 2 cut(s) 472, 504
BpmI CTGGAG 1 cut(s) 753
BpuMI CCSGG 2 cut(s) 399, 1186
BsaBI GATNNNNATC 1 cut(s) 1107
BsaHI GRCGYC 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 433, 816
BsaWI WCCGGW 1 cut(s) 57
Bsc4I CCNNNNNNNGG 3 cut(s) 398, 530, 1126
Bse1I ACTGG 2 cut(s) 137, 1048
Bse3DI GCAATG 2 cut(s) 295, 995
Bse8I GATNNNNATC 1 cut(s) 1107
BseBI CCWGG 2 cut(s) 230, 525
BseDI CCNNGG 2 cut(s) 433, 816
BseGI GGATG 4 cut(s) 558, 721, 989, 1116
BseJI GATNNNNATC 1 cut(s) 1107
BseLI CCNNNNNNNGG 3 cut(s) 398, 530, 1126
BseMI GCAATG 2 cut(s) 295, 995
BseMII CTCAG 3 cut(s) 14, 164, 503
BseNI ACTGG 2 cut(s) 137, 1048
BseRI GAGGAG 1 cut(s) 350
BseSI GKGCMC 1 cut(s) 144
Bsh1236I CGCG 1 cut(s) 239
BshFI GGCC 1 cut(s) 953
BsiHKAI GWGCWC 1 cut(s) 870
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 3 cut(s) 58, 398, 1186
BslI CCNNNNNNNGG 3 cut(s) 398, 530, 1126
BsmAI GTCTC 2 cut(s) 51, 244
BsmBI CGTCTC 2 cut(s) 51, 244
BsmI GAATGC 1 cut(s) 266
BsnI GGCC 1 cut(s) 953
BsoBI CYCGRG 1 cut(s) 815
Bsp1286I GDGCHC 2 cut(s) 144, 870
Bsp143I GATC 8 cut(s) 32, 75, 232, 313, 403, 620, 723, 923
Bsp19I CCATGG 1 cut(s) 433
BspACI CCGC 1 cut(s) 704
BspANI GGCC 1 cut(s) 953
BspCNI CTCAG 3 cut(s) 15, 163, 502
BspFNI CGCG 1 cut(s) 239
BspLI GGNNCC 2 cut(s) 702, 1131
BspMI ACCTGC 1 cut(s) 93
BspPI GGATC 3 cut(s) 240, 321, 718
BspTI CTTAAG 1 cut(s) 193
BsrDI GCAATG 2 cut(s) 295, 995
BsrI ACTGG 2 cut(s) 137, 1048
BssECI CCNNGG 2 cut(s) 433, 816
BssMI GATC 8 cut(s) 32, 75, 232, 313, 403, 620, 723, 923
BssNI GRCGYC 1 cut(s) 44
BssT1I CCWWGG 1 cut(s) 433
Bst2UI CCWGG 2 cut(s) 230, 525
Bst4CI ACNGT 2 cut(s) 443, 1153
Bst6I CTCTTC 1 cut(s) 833
BstACI GRCGYC 1 cut(s) 44
BstAFI CTTAAG 1 cut(s) 193
BstC8I GCNNGC 3 cut(s) 243, 322, 418
BstDEI CTNAG 4 cut(s) 23, 150, 489, 854
BstDSI CCRYGG 1 cut(s) 433
BstF5I GGATG 4 cut(s) 558, 721, 989, 1116
BstFNI CGCG 1 cut(s) 239
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 8 cut(s) 35, 78, 235, 316, 406, 623, 726, 926
BstMAI GTCTC 2 cut(s) 51, 244
BstMBI GATC 8 cut(s) 32, 75, 232, 313, 403, 620, 723, 923
BstMWI GCNNNNNNNGC 2 cut(s) 94, 509
BstNI CCWGG 2 cut(s) 230, 525
BstSCI CCNGG 4 cut(s) 228, 397, 523, 1184
BstSFI CTRYAG 3 cut(s) 183, 859, 936
BstSLI GKGCMC 1 cut(s) 144
BstUI CGCG 1 cut(s) 239
BstX2I RGATCY 1 cut(s) 620
BstYI RGATCY 1 cut(s) 620
BsuRI GGCC 1 cut(s) 953
BtgI CCRYGG 1 cut(s) 433
BtgZI GCGATG 1 cut(s) 199
BtsCI GGATG 4 cut(s) 558, 721, 989, 1116
BtsI GCAGTG 1 cut(s) 1111
BtsIMutI CAGTG 2 cut(s) 144, 1111
BveI ACCTGC 1 cut(s) 93
Cac8I GCNNGC 3 cut(s) 243, 322, 418
CaiI CAGNNNCTG 2 cut(s) 229, 1054
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 1 cut(s) 913
CseI GACGC 1 cut(s) 33
Csp6I GTAC 4 cut(s) 101, 439, 447, 499
CviAII CATG 6 cut(s) 317, 434, 551, 707, 799, 1010
CviQI GTAC 4 cut(s) 101, 439, 447, 499
DdeI CTNAG 4 cut(s) 23, 150, 489, 854
DpnI GATC 8 cut(s) 34, 77, 234, 315, 405, 622, 725, 925
DpnII GATC 8 cut(s) 32, 75, 232, 313, 403, 620, 723, 923
Eam1104I CTCTTC 1 cut(s) 833
EarI CTCTTC 1 cut(s) 833
Ecl136II GAGCTC 1 cut(s) 868
Eco130I CCWWGG 1 cut(s) 433
Eco24I GRGCYC 1 cut(s) 870
Eco47I GGWCC 1 cut(s) 913
Eco53kI GAGCTC 1 cut(s) 868
Eco57I CTGAAG 5 cut(s) 561, 645, 803, 825, 968
Eco88I CYCGRG 1 cut(s) 815
EcoICRI GAGCTC 1 cut(s) 868
EcoRI GAATTC 1 cut(s) 352
EcoRII CCWGG 2 cut(s) 228, 523
EcoT14I CCWWGG 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 870
ErhI CCWWGG 1 cut(s) 433
Esp3I CGTCTC 2 cut(s) 51, 244
FaeI CATG 6 cut(s) 320, 437, 554, 710, 802, 1013
FatI CATG 6 cut(s) 316, 433, 550, 706, 798, 1009
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 1 cut(s) 516
FokI GGATG 4 cut(s) 545, 728, 976, 1103
FriOI GRGCYC 1 cut(s) 870
FspBI CTAG 4 cut(s) 477, 606, 957, 1071
GlaI GCGC 1 cut(s) 240
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 1 cut(s) 953
HapII CCGG 3 cut(s) 58, 398, 1186
HgaI GACGC 1 cut(s) 33
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 6 cut(s) 320, 437, 554, 710, 802, 1013
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HinfI GANTC 3 cut(s) 659, 688, 1181
HpaII CCGG 3 cut(s) 58, 398, 1186
HphI GGTGA 6 cut(s) 27, 47, 413, 1039, 1054, 1141
Hpy166II GTNNAC 1 cut(s) 517
Hpy188III TCNNGA 3 cut(s) 307, 927, 1100
Hpy8I GTNNAC 1 cut(s) 517
HpyAV CCTTC 7 cut(s) 182, 342, 461, 536, 805, 943, 1130
HpyCH4III ACNGT 2 cut(s) 443, 1153
HpyCH4V TGCA 9 cut(s) 88, 280, 378, 453, 512, 550, 752, 973, 1116
HpyF10VI GCNNNNNNNGC 2 cut(s) 94, 509
HpyF3I CTNAG 4 cut(s) 23, 150, 489, 854
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 6 cut(s) 320, 437, 554, 710, 802, 1013
HspAI GCGC 1 cut(s) 239
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 8 cut(s) 32, 75, 232, 313, 403, 620, 723, 923
LmnI GCTCC 2 cut(s) 671, 1129
LweI GCATC 3 cut(s) 333, 753, 1125
MaeI CTAG 4 cut(s) 477, 606, 957, 1071
MaeIII GTNAC 5 cut(s) 160, 676, 890, 1027, 1168
MalI GATC 8 cut(s) 34, 77, 234, 315, 405, 622, 725, 925
MboI GATC 8 cut(s) 32, 75, 232, 313, 403, 620, 723, 923
MboII GAAGA 4 cut(s) 451, 630, 820, 998
MflI RGATCY 1 cut(s) 620
MhlI GDGCHC 2 cut(s) 144, 870
MluCI AATT 9 cut(s) 106, 131, 352, 369, 483, 599, 747, 1002, 1143
MlyI GAGTC 1 cut(s) 668
MmeI TCCRAC 1 cut(s) 957
MnlI CCTC 6 cut(s) 34, 229, 328, 777, 811, 1078
MroXI GAANNNNTTC 1 cut(s) 352
MseI TTAA 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 935
MspCI CTTAAG 1 cut(s) 193
MspI CCGG 3 cut(s) 58, 398, 1186
MspR9I CCNGG 4 cut(s) 230, 399, 525, 1186
Mva1269I GAATGC 1 cut(s) 266
MvaI CCWGG 2 cut(s) 230, 525
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 2 cut(s) 94, 509
NciI CCSGG 2 cut(s) 399, 1186
NcoI CCATGG 1 cut(s) 433
NdeII GATC 8 cut(s) 32, 75, 232, 313, 403, 620, 723, 923
NlaIII CATG 6 cut(s) 320, 437, 554, 710, 802, 1013
NlaIV GGNNCC 2 cut(s) 702, 1131
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 3 cut(s) 160, 676, 1027
PaqCI CACCTGC 1 cut(s) 93
PctI GAATGC 1 cut(s) 266
PdmI GAANNNNTTC 1 cut(s) 352
PfeI GAWTC 2 cut(s) 688, 1181
PflMI CCANNNNNTGG 1 cut(s) 530
PleI GAGTC 1 cut(s) 667
PpsI GAGTC 1 cut(s) 667
PsiI TTATAA 2 cut(s) 650, 909
Psp124BI GAGCTC 1 cut(s) 870
Psp6I CCWGG 2 cut(s) 228, 523
PspGI CCWGG 2 cut(s) 228, 523
PspN4I GGNNCC 2 cut(s) 702, 1131
PspPI GGNCC 1 cut(s) 913
PstNI CAGNNNCTG 2 cut(s) 229, 1054
PsuI RGATCY 1 cut(s) 620
PvuII CAGCTG 1 cut(s) 935
RsaI GTAC 4 cut(s) 102, 440, 448, 500
RsaNI GTAC 4 cut(s) 101, 439, 447, 499
SacI GAGCTC 1 cut(s) 870
SaqAI TTAA 1 cut(s) 194
Sau3AI GATC 8 cut(s) 32, 75, 232, 313, 403, 620, 723, 923
Sau96I GGNCC 1 cut(s) 913
ScaI AGTACT 1 cut(s) 448
SchI GAGTC 1 cut(s) 668
ScrFI CCNGG 4 cut(s) 230, 399, 525, 1186
SduI GDGCHC 2 cut(s) 144, 870
SfaNI GCATC 3 cut(s) 333, 753, 1125
SfcI CTRYAG 3 cut(s) 183, 859, 936
SinI GGWCC 1 cut(s) 913
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
SpeI ACTAGT 1 cut(s) 605
Sse9I AATT 9 cut(s) 106, 131, 352, 369, 483, 599, 747, 1002, 1143
SsiI CCGC 1 cut(s) 704
SspMI CTAG 4 cut(s) 477, 606, 957, 1071
SstI GAGCTC 1 cut(s) 870
StyD4I CCNGG 4 cut(s) 228, 397, 523, 1184
StyI CCWWGG 1 cut(s) 433
TaaI ACNGT 2 cut(s) 443, 1153
TaqI TCGA 3 cut(s) 273, 582, 662
TasI AATT 9 cut(s) 106, 131, 352, 369, 483, 599, 747, 1002, 1143
TatI WGTACW 1 cut(s) 446
TfiI GAWTC 2 cut(s) 688, 1181
Tru1I TTAA 1 cut(s) 194
Tru9I TTAA 1 cut(s) 194
TscAI CASTG 2 cut(s) 144, 1118
TseFI GTSAC 3 cut(s) 160, 676, 1027
Tsp45I GTSAC 3 cut(s) 160, 676, 1027
TspRI CASTG 2 cut(s) 144, 1118
Van91I CCANNNNNTGG 1 cut(s) 530
Vha464I CTTAAG 1 cut(s) 193
VpaK11BI GGWCC 1 cut(s) 913
XapI RAATTY 2 cut(s) 352, 369
XmiI GTMKAC 1 cut(s) 516
XmnI GAANNNNTTC 1 cut(s) 352
XspI CTAG 4 cut(s) 477, 606, 957, 1071
ZrmI AGTACT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.