Prupe.1G312200_v2.0.a1

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001162
Physical Location & Seq
Reverse (-)
30291520 .. 30293997
2478 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G312200.5

Sequence Viewer

Length: 1191 bp
ATGAACCTTTCGCTTACTCTGAGTTTCCGACCAACGGAGCCTTCTCTGTTTGACCGGAAATCCTTCTCTCCGATCAAGTATGCACAAAAACAGCACGTACCCATTTTTCATCAACCAAAATTGGACCCAAGAAGAACTCAGTTCAGTGTCACGGCTAAGAGACCGTCGCCAATAGAAGGCTTAAGCGAGGAGATGAGCGTCATGGCTTCGCAGAATCTAGACCATGCACCTGCTCGGAGACGAGTTCGGTCTGCATTCGTTGACGTGCATCAACAGCTTGATCATTGCTTGTTCAAGATGGATCATGCTGGCATCAGAACAGAGGAGTGGTATGGAAGGAACTCAAAGGGCATGCAAATTTTCTGCAAAAGCTGGTTGCCAAAACTAGATGTTCAAATCAAAGGTGCTTTATGTTTTTGCCATGGGTACGGTAGCACTTGCACATTCTTCTTTGAAGGTATTGCTAAGCGAATTGCTGCATCTGGGTACGCTGTATATGCTGTGGACTATCCAGGTTTTGGTCTTTCTGAAGGATTGCATGGTTACATCCCAAACTTTGATGAATTGGTTGACGATGTTAATGAACAATTTACTAATATCAAAGGAAGACCCGAAGTGAAAGGTTTGCCCTTCTTTATAATGGGAGAGTCCATGGGCGGAGCTGTCACTCTCAAGATTCATCTAAAAGAACCGTCTAAATGGGATGGAGTAATCCTTGTAGCTCCAATGTGTAAAATTGCAGAGGACGTGATGCCTCCAGCAATAGTTCTGAAGCTATTAGCACTCATGTCTGAAGTTTTGCCTCAGGCAAAACTCTTTCCCCAGAAAGACATGGCTGGCCTAAGCTACAGAGATCCGAGAAAGAGAAAAACGGCTGGTTACAATGTGATTTCTTACAAAGACCACATGCGATCAAGAACAGCTGTGGAACTGCTGAAGGCCACAAGTGATATAGAAACACAGTTGGATAAGGTTTCAGGGCCATTGCTAATTCTTCATGGAGCGGCAGATAAGGTGACCGATCCTTTAGTGAGCCAGTTACTTTATGAGAAGGCCTCTAGCAAGGATAAAACTTTGAAGCTCTATCAAGATGGTTATCACTGCATTCTTCAAGGGGAACCTGATGACAGAATTTTTACTGTCCTAGATGACATTGTAACATGGCTTGATTTTCGGTGCTCCCCCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

397

Amino Acids

44.61

Weight (kDa)

8.47

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 638
AarI CACCTGC 1 cut(s) 238
Acc36I ACCTGC 1 cut(s) 238
AccB7I CCANNNNNTGG 1 cut(s) 518
AccBSI CCGCTC 1 cut(s) 1004
AciI CCGC 2 cut(s) 657, 1004
AclWI GGATC 3 cut(s) 309, 848, 1016
AcsI RAATTY 2 cut(s) 357, 1131
AcuI CTGAAG 4 cut(s) 549, 791, 813, 956
AfaI GTAC 3 cut(s) 99, 428, 488
AfiI CCNNNNNNNGG 3 cut(s) 34, 176, 518
AflII CTTAAG 1 cut(s) 181
AgsI TTSAA 5 cut(s) 295, 395, 455, 1078, 1112
AjiI CACGTC 2 cut(s) 265, 748
AjnI CCWGG 1 cut(s) 511
AjuI GAANNNNNNNTTGG 2 cut(s) 25, 57
AluBI AGCT 8 cut(s) 277, 372, 662, 722, 775, 846, 923, 1081
AluI AGCT 8 cut(s) 277, 372, 662, 722, 775, 846, 923, 1081
Alw21I GWGCWC 1 cut(s) 1181
Alw26I GTCTC 2 cut(s) 154, 232
AlwI GGATC 3 cut(s) 309, 848, 1016
AoxI GGCC 4 cut(s) 838, 939, 980, 1053
ApeKI GCWGC 1 cut(s) 476
ApoI RAATTY 2 cut(s) 357, 1131
Asp700I GAANNNNTTC 1 cut(s) 62
AspS9I GGNCC 2 cut(s) 124, 980
AsuHPI GGTGA 1 cut(s) 1027
AvaII GGWCC 1 cut(s) 124
AxyI CCTNAGG 1 cut(s) 804
BbsI GAAGAC 1 cut(s) 613
Bbv12I GWGCWC 1 cut(s) 1181
BbvI GCAGC 1 cut(s) 463
BccI CCATC 3 cut(s) 292, 698, 1085
BceAI ACGGC 2 cut(s) 168, 888
BciT130I CCWGG 1 cut(s) 513
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 2 cut(s) 154, 232
BfaI CTAG 4 cut(s) 218, 386, 1059, 1145
BfmI CTRYAG 1 cut(s) 847
BfrI CTTAAG 1 cut(s) 181
BfuAI ACCTGC 1 cut(s) 238
BisI GCNGC 2 cut(s) 477, 1005
BlpI GCTNAGC 1 cut(s) 465
BlsI GCNGC 2 cut(s) 478, 1006
Bme1390I CCNGG 1 cut(s) 513
Bme18I GGWCC 1 cut(s) 124
BmgBI CACGTC 2 cut(s) 265, 748
BmgT120I GGNCC 2 cut(s) 124, 980
BmiI GGNNCC 3 cut(s) 39, 126, 1119
BmrFI CCNGG 1 cut(s) 513
BmsI GCATC 4 cut(s) 277, 321, 488, 741
BpiI GAAGAC 1 cut(s) 613
BplI GAGNNNNNCTC 2 cut(s) 1040, 1072
BpmI CTGGAG 1 cut(s) 741
Bpu10I CCTNAGC 1 cut(s) 842
Bpu1102I GCTNAGC 1 cut(s) 465
BpuEI CTTGAG 1 cut(s) 656
BsaAI YACGTR 1 cut(s) 97
BsaBI GATNNNNATC 1 cut(s) 1095
BsaI GGTCTC 1 cut(s) 154
BsaJI CCNNGG 2 cut(s) 421, 651
BsaWI WCCGGW 1 cut(s) 54
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 176, 518
Bse1I ACTGG 1 cut(s) 1036
Bse21I CCTNAGG 1 cut(s) 804
Bse3DI GCAATG 2 cut(s) 283, 983
Bse8I GATNNNNATC 1 cut(s) 1095
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 2 cut(s) 421, 651
BseGI GGATG 2 cut(s) 546, 709
BseJI GATNNNNATC 1 cut(s) 1095
BseLI CCNNNNNNNGG 3 cut(s) 34, 176, 518
BseMI GCAATG 2 cut(s) 283, 983
BseMII CTCAG 3 cut(s) 11, 152, 818
BseNI ACTGG 1 cut(s) 1036
BseRI GAGGAG 2 cut(s) 203, 338
BseXI GCAGC 1 cut(s) 463
BshFI GGCC 4 cut(s) 840, 941, 982, 1055
BsiHKAI GWGCWC 1 cut(s) 1181
BsiSI CCGG 1 cut(s) 55
BslI CCNNNNNNNGG 3 cut(s) 34, 176, 518
BsmAI GTCTC 2 cut(s) 154, 232
BsmBI CGTCTC 1 cut(s) 232
BsmI GAATGC 2 cut(s) 254, 1104
BsnI GGCC 4 cut(s) 840, 941, 982, 1055
Bso31I GGTCTC 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 1181
Bsp143I GATC 6 cut(s) 72, 280, 301, 853, 911, 1021
Bsp1720I GCTNAGC 1 cut(s) 465
Bsp19I CCATGG 2 cut(s) 421, 651
BspACI CCGC 2 cut(s) 657, 1004
BspANI GGCC 4 cut(s) 840, 941, 982, 1055
BspCNI CTCAG 3 cut(s) 12, 151, 817
BspLI GGNNCC 3 cut(s) 39, 126, 1119
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 3 cut(s) 309, 848, 1016
BspTI CTTAAG 1 cut(s) 181
BspTNI GGTCTC 1 cut(s) 154
BsrBI CCGCTC 1 cut(s) 1004
BsrDI GCAATG 2 cut(s) 283, 983
BsrI ACTGG 1 cut(s) 1036
BssECI CCNNGG 2 cut(s) 421, 651
BssMI GATC 6 cut(s) 72, 280, 301, 853, 911, 1021
BssT1I CCWWGG 2 cut(s) 421, 651
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 5 cut(s) 165, 431, 693, 963, 1141
BstAFI CTTAAG 1 cut(s) 181
BstBAI YACGTR 1 cut(s) 97
BstC8I GCNNGC 3 cut(s) 310, 353, 838
BstDEI CTNAG 6 cut(s) 20, 138, 156, 465, 804, 842
BstDSI CCRYGG 2 cut(s) 421, 651
BstEII GGTNACC 1 cut(s) 1015
BstF5I GGATG 2 cut(s) 546, 709
BstKTI GATC 6 cut(s) 75, 283, 304, 856, 914, 1024
BstMAI GTCTC 2 cut(s) 154, 232
BstMBI GATC 6 cut(s) 72, 280, 301, 853, 911, 1021
BstMWI GCNNNNNNNGC 2 cut(s) 274, 497
BstNI CCWGG 1 cut(s) 513
BstNSI RCATGY 2 cut(s) 355, 910
BstPI GGTNACC 1 cut(s) 1015
BstSCI CCNGG 1 cut(s) 511
BstSFI CTRYAG 1 cut(s) 847
BstV1I GCAGC 1 cut(s) 463
BstV2I GAAGAC 1 cut(s) 613
BstX2I RGATCY 1 cut(s) 853
BstYI RGATCY 1 cut(s) 853
Bsu36I CCTNAGG 1 cut(s) 804
BsuRI GGCC 4 cut(s) 840, 941, 982, 1055
BtgI CCRYGG 2 cut(s) 421, 651
BtrI CACGTC 2 cut(s) 265, 748
BtsCI GGATG 2 cut(s) 546, 709
BtsI GCAGTG 1 cut(s) 1099
BtsIMutI CAGTG 2 cut(s) 151, 1099
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 3 cut(s) 310, 353, 838
Cfr13I GGNCC 2 cut(s) 124, 980
CseI GACGC 1 cut(s) 187
Csp6I GTAC 3 cut(s) 98, 427, 487
CviQI GTAC 3 cut(s) 98, 427, 487
DdeI CTNAG 6 cut(s) 20, 138, 156, 465, 804, 842
DpnI GATC 6 cut(s) 74, 282, 303, 855, 913, 1023
DpnII GATC 6 cut(s) 72, 280, 301, 853, 911, 1021
EciI GGCGGA 1 cut(s) 672
Eco130I CCWWGG 2 cut(s) 421, 651
Eco147I AGGCCT 1 cut(s) 1055
Eco31I GGTCTC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 124
Eco57I CTGAAG 4 cut(s) 549, 791, 813, 956
Eco81I CCTNAGG 1 cut(s) 804
Eco91I GGTNACC 1 cut(s) 1015
EcoO65I GGTNACC 1 cut(s) 1015
EcoRII CCWGG 1 cut(s) 511
EcoT14I CCWWGG 2 cut(s) 421, 651
ErhI CCWWGG 2 cut(s) 421, 651
Esp3I CGTCTC 1 cut(s) 232
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 2 cut(s) 477, 1005
FokI GGATG 2 cut(s) 533, 716
Fsp4HI GCNGC 2 cut(s) 477, 1005
FspBI CTAG 4 cut(s) 218, 386, 1059, 1145
GluI GCNGC 2 cut(s) 477, 1005
GsuI CTGGAG 1 cut(s) 741
HaeIII GGCC 4 cut(s) 840, 941, 982, 1055
HapII CCGG 1 cut(s) 55
HgaI GACGC 1 cut(s) 187
HincII GTYRAC 2 cut(s) 262, 571
HindII GTYRAC 2 cut(s) 262, 571
HinfI GANTC 3 cut(s) 214, 647, 676
HpaII CCGG 1 cut(s) 55
HphI GGTGA 1 cut(s) 1027
Hpy166II GTNNAC 3 cut(s) 262, 505, 571
Hpy188I TCNGA 9 cut(s) 21, 29, 72, 237, 317, 529, 771, 793, 858
Hpy188III TCNNGA 5 cut(s) 218, 295, 673, 915, 1088
Hpy8I GTNNAC 3 cut(s) 262, 505, 571
Hpy99I CGWCG 1 cut(s) 169
HpyAV CCTTC 9 cut(s) 51, 73, 170, 330, 449, 524, 640, 931, 1045
HpyCH4III ACNGT 5 cut(s) 165, 431, 693, 963, 1141
HpyCH4IV ACGT 3 cut(s) 96, 264, 747
HpyF10VI GCNNNNNNNGC 2 cut(s) 274, 497
HpyF3I CTNAG 6 cut(s) 20, 138, 156, 465, 804, 842
HpySE526I ACGT 3 cut(s) 96, 264, 747
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 6 cut(s) 72, 280, 301, 853, 911, 1021
LmnI GCTCC 5 cut(s) 37, 659, 727, 1001, 1184
Lsp1109I GCAGC 1 cut(s) 463
LweI GCATC 4 cut(s) 277, 321, 488, 741
MaeI CTAG 4 cut(s) 218, 386, 1059, 1145
MaeII ACGT 3 cut(s) 96, 264, 747
MaeIII GTNAC 7 cut(s) 148, 542, 664, 878, 1015, 1038, 1156
MalI GATC 6 cut(s) 74, 282, 303, 855, 913, 1023
MbiI CCGCTC 1 cut(s) 1004
MboI GATC 6 cut(s) 72, 280, 301, 853, 911, 1021
MboII GAAGA 5 cut(s) 144, 439, 618, 986, 1100
MflI RGATCY 1 cut(s) 853
MhlI GDGCHC 1 cut(s) 1181
MluCI AATT 9 cut(s) 119, 357, 471, 563, 587, 735, 990, 1131, 1186
MlyI GAGTC 1 cut(s) 656
MmeI TCCRAC 2 cut(s) 52, 945
MnlI CCTC 6 cut(s) 181, 316, 736, 765, 813, 1066
MroXI GAANNNNTTC 1 cut(s) 62
MseI TTAA 3 cut(s) 182, 579, 1189
MspA1I CMGCKG 1 cut(s) 923
MspCI CTTAAG 1 cut(s) 181
MspI CCGG 1 cut(s) 55
MspR9I CCNGG 1 cut(s) 513
Mva1269I GAATGC 2 cut(s) 254, 1104
MvaI CCWGG 1 cut(s) 513
MwoI GCNNNNNNNGC 2 cut(s) 274, 497
NcoI CCATGG 2 cut(s) 421, 651
NdeII GATC 6 cut(s) 72, 280, 301, 853, 911, 1021
NlaIV GGNNCC 3 cut(s) 39, 126, 1119
NmuCI GTSAC 3 cut(s) 148, 664, 1015
NspI RCATGY 2 cut(s) 355, 910
PaeI GCATGC 1 cut(s) 355
PaqCI CACCTGC 1 cut(s) 238
PceI AGGCCT 1 cut(s) 1055
PctI GAATGC 2 cut(s) 254, 1104
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 2 cut(s) 214, 676
PflMI CCANNNNNTGG 1 cut(s) 518
PkrI GCNGC 2 cut(s) 478, 1006
PleI GAGTC 1 cut(s) 655
PpsI GAGTC 1 cut(s) 655
Ppu21I YACGTR 1 cut(s) 97
PsiI TTATAA 1 cut(s) 638
Psp6I CCWGG 1 cut(s) 511
PspEI GGTNACC 1 cut(s) 1015
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 3 cut(s) 39, 126, 1119
PspPI GGNCC 2 cut(s) 124, 980
PsuI RGATCY 1 cut(s) 853
PvuII CAGCTG 1 cut(s) 923
RsaI GTAC 3 cut(s) 99, 428, 488
RsaNI GTAC 3 cut(s) 98, 427, 487
SaqAI TTAA 3 cut(s) 182, 579, 1189
SatI GCNGC 2 cut(s) 477, 1005
Sau3AI GATC 6 cut(s) 72, 280, 301, 853, 911, 1021
Sau96I GGNCC 2 cut(s) 124, 980
SchI GAGTC 1 cut(s) 656
ScrFI CCNGG 1 cut(s) 513
SduI GDGCHC 1 cut(s) 1181
SfaNI GCATC 4 cut(s) 277, 321, 488, 741
SfcI CTRYAG 1 cut(s) 847
SinI GGWCC 1 cut(s) 124
SmlI CTYRAG 2 cut(s) 181, 671
SmoI CTYRAG 2 cut(s) 181, 671
SphI GCATGC 1 cut(s) 355
Sse9I AATT 9 cut(s) 119, 357, 471, 563, 587, 735, 990, 1131, 1186
SseBI AGGCCT 1 cut(s) 1055
SsiI CCGC 2 cut(s) 657, 1004
SspMI CTAG 4 cut(s) 218, 386, 1059, 1145
StuI AGGCCT 1 cut(s) 1055
StyD4I CCNGG 1 cut(s) 511
StyI CCWWGG 2 cut(s) 421, 651
TaaI ACNGT 5 cut(s) 165, 431, 693, 963, 1141
TaiI ACGT 3 cut(s) 99, 267, 750
TaqII GACCGA 2 cut(s) 237, 1034
TasI AATT 9 cut(s) 119, 357, 471, 563, 587, 735, 990, 1131, 1186
TauI GCSGC 1 cut(s) 1007
TfiI GAWTC 2 cut(s) 214, 676
Tru1I TTAA 3 cut(s) 182, 579, 1189
Tru9I TTAA 3 cut(s) 182, 579, 1189
TscAI CASTG 2 cut(s) 151, 1106
TseFI GTSAC 3 cut(s) 148, 664, 1015
TseI GCWGC 1 cut(s) 476
Tsp45I GTSAC 3 cut(s) 148, 664, 1015
TspDTI ATGAA 6 cut(s) 17, 98, 576, 597, 668, 986
TspGWI ACGGA 1 cut(s) 50
TspRI CASTG 2 cut(s) 151, 1106
Van91I CCANNNNNTGG 1 cut(s) 518
Vha464I CTTAAG 1 cut(s) 181
VpaK11BI GGWCC 1 cut(s) 124
XapI RAATTY 2 cut(s) 357, 1131
XbaI TCTAGA 1 cut(s) 217
XceI RCATGY 2 cut(s) 355, 910
XmnI GAANNNNTTC 1 cut(s) 62
XspI CTAG 4 cut(s) 218, 386, 1059, 1145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.