Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0000187

Physical Location & Seq

Genomic Coordinates Reverse (-)

9911857 .. 9915045

Gene Structure 3189 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0122900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2103 bp

ATGAGCAACCCAAACTCCCACTTCCCGAAATCCCTGTCCCCGAAGCGCGTCCTCAAGCTCCTCCAAGCCGAAAAGAACACCCACTCCGCACTCGCCCTCCTCGACTCCGCCACTCGCCACCCCAACTACACCCACTCCCCCGACGTCTTCCACCACATCCTCCGCCGCCTCATCCACCCCAACCTCGTCGCCCACGTCGGTCGGGTCGTCGAACTCATCCGCACCCAGAAATGCCACTGCCCCGAGGACGTGGCACTGACCGTGATCAAAGCCTACGCCAAAAACTCAATGCCTGATAAAGCCCTGCAGGTCTTCCAGCAAATGCGAGAGATTTTCGGGTGCGAGCCCGGCATTCGATCCTACAATTCTCTTCTAAACTCCTTCATTGAGTCCAACCAGTGGGACCGGGCCGAGCAGTTCTTTGCTTATTTCGAAACGGTGGGGTTGTCCCCAAATTTGCAGACGTACAACACTCTGATCAAGATTTCGTGCAAGAAGAAGCAGTTCGAGAAGGCCAGGGGGTTGCTGGATTGGATGTGGGAGAAGGGTTTGGAGCCTGATGTGATGAGTTACGCAACTTTGATCAATGCGTTTGCGAAAAATGGGAAGCTGGGGGATGCATTGGAGGTGTTCGACGAAATGCCTGAGAGAGGAGTCAGTCCTGATGTCATGTGTTATAATGTTTTGATTGATGGGTTTTTTCGGAAAGGCGAATATGTGGAGGCCAAGGAGGTTTGGGAGAGGTTGGTGATGGACTCGGAGGCTTATCCGAATGTTGTTAGTTATAATGTTATGATTAGTGGCTTGTGCAAATGCGGGAGGTTTGGTGAGAGTTTGGAGATATGGAATAGGATGAAGAGGAATGAAAGAGGATGTGATTTGTTTACTTGTAGTTCTTTGATTCATGGGTTGTGTAAAGCGGGGAATGTGGATGAGGCGGAGAGAGTTTATAAGGATATGGTTGAGAAGGGAGTAGTGCCGGATGTGGTTATGTATAATGCAATGCTCGATGGTTTCTGCCGAGCAGGGAAGATTGGACAGTGCTTTGAGTTGTGGGAGGTGATGGAGAAGGGTGGTTGTCGTAATGTTGTGAGTTATAACATATTGATCAAAGGGTTGTTTGAAAATAAGAAGGCAGAGGAAGCAATGTCTGTCTGGGAATTAATGCATGTGAAGGCTTGTGTTGCAGATTCCACAACGTATGGAGTCTTAATTCATGGACTGTGTAAGAATGGGTACTTGAACAAGGCTCTATGGATTTTAAAAGAGGCGGAAAATGCAAGGGCTGATCTTGATGTCTTTGCATATTCCTCACTGATTAGTTGGTTATGCAAAGAAGGGAGACTAGATGAAGCAGCCAGAATACTTGATCAGATGGTTAAGTGTGGCTATAAACCGAATTCTCATGTTTGCAATTCATTGATATATGGCTTTATCCAAGCTACCAAACTTGAAGATGCAATTTGCTTTTTTAGGGGAATGTCCACCAAGTATTGCTCTCCAAATGTTGTCTCCTACAATACTCTGATAAATGGCTTATGCAAAGTGGGAAGATTTAGTGACGCATATGTGTTTGTGAGGGAAATGCTGGAGACAGGCTGGACACTAGATGTGATCACATATAGTTTGTTAATTGATGGGCTTTGTCAAGGCAGGAAGATTGACATGGCCCTCAATTTGTGGAATCAAGCCCTTGACAAAGGATTCGAACCTGATGTAACTATGTACAACATTATGATTCATGGACTTTGCTCTGCAGGCAAAGCTGAAGGTGCTTTGCAGCTATATTTTCAGATGGGGTGTAGGAACTGTGTTCCGAATCTTGTGACACACAACACCCTAATGGAGGGTTTTTACAAAATTAGAGACTGTGGAAAGGCATCAGAAATTTGGGCTCGCATTTTAAAGAATGGGCTACAACCAGATATAATTTCCTATAATATTACTCTGAAAGGGCTTTGTTCATGCAGTAGAATATCAGATGCTGTTGGGCACTTAGAAAAAGCTTTGCATCATGGAGTTCTTCCAACTCATATAACGTGGCACATACTTGTTAGGGCAGTGGTCGACAACGGGGCCACTATGCAGTCTTCTTGGGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006952 GO:0006979 GO:0008150 GO:0008152 GO:0009505 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0016020 GO:0016491 GO:0016614 GO:0016899 GO:0030054 GO:0030312 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0055114 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BBE FAD_binding_4

Protein Analysis

700

Amino Acids

79.04

Weight (kDa)

7.45

Isoelectric Point (pI)

35.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	84 - 213	2.6e-07	Fungal tetratrico peptide repeats
PPR_2	PF13041	87 - 128	2.5e-06	PPR repeat family
PPR_2	PF13041	116 - 165	5e-09	PPR repeat family
PPR_long	PF17177	135 - 238	5.6e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	151 - 200	3.8e-13	PPR repeat family
PPR_3	PF13812	175 - 231	2.8e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	182 - 214	1.9e-11	PPR repeat
PPR_2	PF13041	186 - 234	7.2e-16	PPR repeat family
PPR	PF01535	190 - 219	7.5e-08	PPR repeat
PPR_1	PF12854	217 - 248	1.8e-07	PPR repeat
PPR_2	PF13041	257 - 306	9.7e-15	PPR repeat family
PPR_1	PF12854	257 - 286	1.2e-08	PPR repeat
PPR	PF01535	260 - 288	2e-08	PPR repeat
PPR_long	PF17177	277 - 357	8.7e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	283 - 337	5.4e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	293 - 330	8.6e-10	PPR repeat family
PPR_1	PF12854	293 - 321	2.7e-08	PPR repeat
PPR	PF01535	295 - 325	4.9e-09	PPR repeat
PPR_3	PF13812	315 - 371	3.2e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	323 - 356	4.2e-10	PPR repeat
PPR_2	PF13041	327 - 360	5.6e-09	PPR repeat family
PPR	PF01535	330 - 360	1.1e-06	PPR repeat
PPR_2	PF13041	361 - 410	2e-14	PPR repeat family
PPR_1	PF12854	394 - 423	2.4e-06	PPR repeat
PPR_1	PF12854	431 - 460	4.2e-09	PPR repeat
PPR_2	PF13041	432 - 478	1.5e-11	PPR repeat family
PPR	PF01535	435 - 463	7.8e-07	PPR repeat
PPR_3	PF13812	454 - 511	1.6e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	466 - 515	1.2e-12	PPR repeat family
PPR_1	PF12854	499 - 529	4.2e-13	PPR repeat
PPR_2	PF13041	501 - 550	9.5e-17	PPR repeat family
PPR	PF01535	504 - 532	2.2e-06	PPR repeat
PPR_2	PF13041	544 - 584	1.2e-09	PPR repeat family
PPR_3	PF13812	564 - 616	6e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	567 - 599	1.5e-08	PPR repeat
PPR_2	PF13041	571 - 620	8.7e-14	PPR repeat family
PPR	PF01535	575 - 604	3.5e-06	PPR repeat
PPR_2	PF13041	606 - 654	7.6e-10	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000187)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g20170 FvH4_4g06831
rosa_chinensis	RchiOBHm_Chr1g0321991 RchiOBHm_Chr2g0161811
rosa_laevigata	RLG00000015375 RLG00000018377
rosa_multiflora	Rmu_co8011754.1_g000001 Rmu_co8019986.1_g000001 Rmu_co8067766.1_g000001 Rmu_co8156128.1_g000001 Rmu_co8219154.1_g000003 Rmu_co8297235.1_g000001 Rmu_co8345223.1_g000001 Rmu_co8364775.1_g000001 Rmu_co8465075.1_g000001 Rmu_sc0000007.1_g000002 Rmu_sc0000054.1_g000015 Rmu_sc0000054.1_g000023 Rmu_sc0000145.1_g000010 Rmu_sc0000166.1_g000006 Rmu_sc0000218.1_g000012 Rmu_sc0000339.1_g000008 Rmu_sc0000342.1_g000015 Rmu_sc0000464.1_g000008 Rmu_sc0000620.1_g000003 Rmu_sc0000638.1_g000017 Rmu_sc0000890.1_g000007 Rmu_sc0000894.1_g000024 Rmu_sc0000909.1_g000010 Rmu_sc0000928.1_g000020 Rmu_sc0000977.1_g000007 Rmu_sc0001022.1_g000003 Rmu_sc0001035.1_g000037 Rmu_sc0001060.1_g000008 Rmu_sc0001168.1_g000005 Rmu_sc0001173.1_g000022 Rmu_sc0001179.1_g000016 Rmu_sc0001408.1_g000003 Rmu_sc0001455.1_g000005 Rmu_sc0001484.1_g000008 Rmu_sc0001585.1_g000010 Rmu_sc0001702.1_g000030 Rmu_sc0001755.1_g000063 Rmu_sc0001903.1_g000009 Rmu_sc0002099.1_g000001 Rmu_sc0002142.1_g000012 Rmu_sc0002158.1_g000014 Rmu_sc0002307.1_g000009 Rmu_sc0002358.1_g000013 Rmu_sc0002421.1_g000002 Rmu_sc0002426.1_g000003 Rmu_sc0002438.1_g000001 Rmu_sc0002461.1_g000001 Rmu_sc0002508.1_g000003 Rmu_sc0002532.1_g000056 Rmu_sc0002583.1_g000002 Rmu_sc0002927.1_g000033 Rmu_sc0003033.1_g000040 Rmu_sc0003271.1_g000006 Rmu_sc0003381.1_g000006 Rmu_sc0003507.1_g000007 Rmu_sc0003578.1_g000003 Rmu_sc0003693.1_g000004 Rmu_sc0003693.1_g000005 Rmu_sc0003704.1_g000003 Rmu_sc0003840.1_g000002 Rmu_sc0004084.1_g000026 Rmu_sc0004193.1_g000007 Rmu_sc0004234.1_g000033 Rmu_sc0004235.1_g000025 Rmu_sc0004435.1_g000006 Rmu_sc0004727.1_g000003 Rmu_sc0004741.1_g000001 Rmu_sc0004926.1_g000004 Rmu_sc0005365.1_g000001 Rmu_sc0005392.1_g000007 Rmu_sc0005415.1_g000001 Rmu_sc0005539.1_g000007 Rmu_sc0005708.1_g000017 Rmu_sc0006317.1_g000007 Rmu_sc0006453.1_g000054 Rmu_sc0006536.1_g000004 Rmu_sc0006740.1_g000022 Rmu_sc0006763.1_g000012 Rmu_sc0007115.1_g000002 Rmu_sc0007115.1_g000010 Rmu_sc0007129.1_g000017 Rmu_sc0007131.1_g000010 Rmu_sc0008373.1_g000008 Rmu_sc0009005.1_g000008 Rmu_sc0009252.1_g000007 Rmu_sc0009599.1_g000022 Rmu_sc0009785.1_g000027 Rmu_sc0009891.1_g000003 Rmu_sc0010099.1_g000010 Rmu_sc0010266.1_g000008 Rmu_sc0010334.1_g000003 Rmu_sc0010381.1_g000001 Rmu_sc0010496.1_g000003 Rmu_sc0010496.1_g000004 Rmu_sc0010546.1_g000003 Rmu_sc0011482.1_g000006 Rmu_sc0011875.1_g000010 Rmu_sc0011917.1_g000006 Rmu_sc0012293.1_g000004 Rmu_sc0012847.1_g000003 Rmu_sc0013773.1_g000001 Rmu_sc0016060.1_g000003 Rmu_sc0016170.1_g000013 Rmu_sc0016393.1_g000002 Rmu_sc0017589.1_g000001 Rmu_sc0017786.1_g000005 Rmu_sc0018326.1_g000002 Rmu_sc0020760.1_g000001 Rmu_sc0021262.1_g000003 Rmu_sc0023851.1_g000002 Rmu_sc0024044.1_g000001 Rmu_sc0026820.1_g000001 Rmu_sc0032306.1_g000001 Rmu_sc0035666.1_g000001 Rmu_sc0036881.1_g000001 Rmu_sc0037030.1_g000001 Rmu_sc0037158.1_g000001 Rmu_ssc0000016.1_g000002 Rmu_ssc0000044.1_g000012 Rmu_ssc0000044.1_g000014 Rmu_ssc0000050.1_g000064 Rmu_ssc0000064.1_g000022 Rmu_ssc0000154.1_g000037 Rmu_ssc0000178.1_g000013 Rmu_ssc0000217.1_g000022 Rmu_ssc0000392.1_g000004
rosa_roxburghii	Rroxscaffold_3G00242000 Rroxscaffold_3G00242530 Rroxscaffold_6G00406010
rosa_rugosa	Rorug01G0062000 Rorug02G0224000 Rorug02G0262900 Rorug02G0263000 Rorug02G0358900 Rorug02G0360500 Rorug02G0378500.1 Rorug02G0378600 Rorug02G0394700 Rorug03G0122900 Rorug03G0254600 Rorug03G0254700 Rorug03G0254800 Rorug04G0042200.1 Rorug04G0378100 Rorug05G0053400 Rorug05G0238600 Rorug05G0364500 Rorug05G0562000 Rorug06G0089300 Rorug06G0089400 Rorug06G0145700 Rorug07G0111600 Rorug07G0229500.1 Rorug07G0287600

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	678, 786, 951, 1098
AatII	GACGTC	1 cut(s)	147
Acc36I	ACCTGC	1 cut(s)	298
AccB7I	CCANNNNNTGG	1 cut(s)	399
AccI	GTMKAC	1 cut(s)	2067
AccII	CGCG	1 cut(s)	48
AciI	CCGC	9 cut(s)	87, 108, 163, 166, 220, 816, 920, 938, 1271
AclWI	GGATC	1 cut(s)	351
AcsI	RAATTY	3 cut(s)	454, 1399, 1887
AcuI	CTGAAG	1 cut(s)	1788
AcyI	GRCGYC	1 cut(s)	144
AfaI	GTAC	3 cut(s)	467, 1238, 1727
AfiI	CCNNNNNNNGG	3 cut(s)	399, 650, 1846
AgsI	TTSAA	3 cut(s)	1124, 1243, 1454
AjiI	CACGTC	2 cut(s)	196, 250
AjnI	CCWGG	1 cut(s)	515
AloI	GAACNNNNNNTCC	2 cut(s)	68, 100
AluBI	AGCT	6 cut(s)	58, 610, 1442, 1766, 1783, 2006
AluI	AGCT	6 cut(s)	58, 610, 1442, 1766, 1783, 2006
Alw26I	GTCTC	4 cut(s)	1336, 1516, 1586, 1860
AlwI	GGATC	1 cut(s)	351
AlwNI	CAGNNNCTG	1 cut(s)	1985
Ama87I	CYCGRG	1 cut(s)	242
AoxI	GGCC	5 cut(s)	408, 513, 723, 1668, 2076
ApeKI	GCWGC	2 cut(s)	1355, 1780
ApoI	RAATTY	3 cut(s)	454, 1399, 1887
ArsI	GACNNNNNNTTYG	4 cut(s)	20, 52, 1688, 1720
AseI	ATTAAT	1 cut(s)	1163
Asp700I	GAANNNNTTC	1 cut(s)	503
AspLEI	GCGC	1 cut(s)	48
AspS9I	GGNCC	4 cut(s)	403, 408, 1669, 2076
AsuC2I	CCSGG	2 cut(s)	348, 407
AsuHPI	GGTGA	3 cut(s)	760, 839, 1072
AsuII	TTCGAA	2 cut(s)	432, 1707
AvaI	CYCGRG	1 cut(s)	242
AvaII	GGWCC	1 cut(s)	403
BaeGI	GKGCMC	1 cut(s)	1995
BanII	GRGCYC	2 cut(s)	348, 1897
BbsI	GAAGAC	3 cut(s)	139, 304, 2082
BbvI	GCAGC	2 cut(s)	1367, 1792
BccI	CCATC	7 cut(s)	686, 745, 1004, 1057, 1369, 1631, 1789
BciT130I	CCWGG	1 cut(s)	517
BclI	TGATCA	6 cut(s)	264, 477, 582, 1107, 1369, 1614
BcnI	CCSGG	2 cut(s)	348, 407
BcoDI	GTCTC	4 cut(s)	1336, 1516, 1586, 1860
BfaI	CTAG	2 cut(s)	1346, 1607
BfmI	CTRYAG	2 cut(s)	305, 1755
BfuAI	ACCTGC	1 cut(s)	298
BisI	GCNGC	3 cut(s)	166, 1356, 1781
BlsI	GCNGC	3 cut(s)	167, 1357, 1782
Bme1390I	CCNGG	3 cut(s)	348, 407, 517
Bme18I	GGWCC	1 cut(s)	403
BmeT110I	CYCGRG	1 cut(s)	242
BmgBI	CACGTC	2 cut(s)	196, 250
BmgT120I	GGNCC	4 cut(s)	403, 408, 1669, 2076
BmiI	GGNNCC	3 cut(s)	404, 555, 2077
BmrFI	CCNGG	3 cut(s)	348, 407, 517
BmsI	GCATC	5 cut(s)	607, 1447, 1889, 1972, 2020
BpiI	GAAGAC	3 cut(s)	139, 304, 2082
BpmI	CTGGAG	1 cut(s)	1610
Bpu14I	TTCGAA	2 cut(s)	432, 1707
BpuEI	CTTGAG	1 cut(s)	38
BpuMI	CCSGG	2 cut(s)	348, 407
BsaHI	GRCGYC	1 cut(s)	144
BsaJI	CCNNGG	3 cut(s)	243, 516, 726
BsaXI	ACNNNNNCTCC	9 cut(s)	29, 68, 81, 98, 111, 119, 149, 811, 841
Bsc4I	CCNNNNNNNGG	3 cut(s)	399, 650, 1846
Bse1I	ACTGG	1 cut(s)	397
Bse3DI	GCAATG	2 cut(s)	1008, 1152
BseBI	CCWGG	1 cut(s)	517
BseDI	CCNNGG	3 cut(s)	243, 516, 726
BseGI	GGATG	9 cut(s)	156, 171, 216, 540, 622, 858, 878, 937, 988
BseLI	CCNNNNNNNGG	3 cut(s)	399, 650, 1846
BseMI	GCAATG	2 cut(s)	1008, 1152
BseMII	CTCAG	1 cut(s)	636
BseNI	ACTGG	1 cut(s)	397
BseRI	GAGGAG	3 cut(s)	50, 89, 666
BseSI	GKGCMC	1 cut(s)	1995
BseXI	GCAGC	2 cut(s)	1367, 1792
BseYI	CCCAGC	1 cut(s)	610
Bsh1236I	CGCG	1 cut(s)	48
Bsh1285I	CGRYCG	1 cut(s)	202
BshFI	GGCC	5 cut(s)	410, 515, 725, 1670, 2078
BsiEI	CGRYCG	1 cut(s)	202
BsiHKCI	CYCGRG	1 cut(s)	242
BsiSI	CCGG	3 cut(s)	348, 406, 980
BslFI	GGGAC	3 cut(s)	22, 416, 433
BslI	CCNNNNNNNGG	3 cut(s)	399, 650, 1846
BsmAI	GTCTC	4 cut(s)	1336, 1516, 1586, 1860
BsmFI	GGGAC	3 cut(s)	22, 416, 433
BsmI	GAATGC	1 cut(s)	351
BsnI	GGCC	5 cut(s)	410, 515, 725, 1670, 2078
BsoBI	CYCGRG	1 cut(s)	242
Bsp119I	TTCGAA	2 cut(s)	432, 1707
Bsp1286I	GDGCHC	3 cut(s)	348, 1897, 1995
Bsp1407I	TGTACA	1 cut(s)	1725
Bsp143I	GATC	8 cut(s)	264, 356, 477, 582, 1107, 1288, 1369, 1614
BspACI	CCGC	9 cut(s)	87, 108, 163, 166, 220, 816, 920, 938, 1271
BspANI	GGCC	5 cut(s)	410, 515, 725, 1670, 2078
BspCNI	CTCAG	1 cut(s)	637
BspFNI	CGCG	1 cut(s)	48
BspLI	GGNNCC	3 cut(s)	404, 555, 2077
BspMAI	CTGCAG	2 cut(s)	309, 1759
BspMI	ACCTGC	1 cut(s)	298
BspPI	GGATC	1 cut(s)	351
BspT104I	TTCGAA	2 cut(s)	432, 1707
BsrDI	GCAATG	2 cut(s)	1008, 1152
BsrGI	TGTACA	1 cut(s)	1725
BsrI	ACTGG	1 cut(s)	397
BssECI	CCNNGG	3 cut(s)	243, 516, 726
BssMI	GATC	8 cut(s)	264, 356, 477, 582, 1107, 1288, 1369, 1614
BssNI	GRCGYC	1 cut(s)	144
BssT1I	CCWWGG	1 cut(s)	726
Bst2UI	CCWGG	1 cut(s)	517
Bst4CI	ACNGT	6 cut(s)	262, 439, 1041, 1224, 1811, 1871
Bst6I	CTCTTC	2 cut(s)	375, 851
BstACI	GRCGYC	1 cut(s)	144
BstAUI	TGTACA	1 cut(s)	1725
BstBI	TTCGAA	2 cut(s)	432, 1707
BstC8I	GCNNGC	3 cut(s)	344, 1759, 1897
BstDEI	CTNAG	2 cut(s)	645, 1996
BstENI	CCTNNNNNAGG	2 cut(s)	648, 1844
BstF5I	GGATG	9 cut(s)	156, 171, 216, 540, 622, 858, 878, 937, 988
BstFNI	CGCG	1 cut(s)	48
BstHHI	GCGC	1 cut(s)	48
BstKTI	GATC	8 cut(s)	267, 359, 480, 585, 1110, 1291, 1372, 1617
BstMAI	GTCTC	4 cut(s)	1336, 1516, 1586, 1860
BstMBI	GATC	8 cut(s)	264, 356, 477, 582, 1107, 1288, 1369, 1614
BstMCI	CGRYCG	1 cut(s)	202
BstMWI	GCNNNNNNNGC	7 cut(s)	348, 1142, 1184, 1277, 1758, 1763, 1772
BstNI	CCWGG	1 cut(s)	517
BstNSI	RCATGY	1 cut(s)	1172
BstSCI	CCNGG	3 cut(s)	346, 405, 515
BstSFI	CTRYAG	2 cut(s)	305, 1755
BstSLI	GKGCMC	1 cut(s)	1995
BstUI	CGCG	1 cut(s)	48
BstV1I	GCAGC	2 cut(s)	1367, 1792
BstV2I	GAAGAC	3 cut(s)	139, 304, 2082
BsuRI	GGCC	5 cut(s)	410, 515, 725, 1670, 2078
BtrI	CACGTC	2 cut(s)	196, 250
BtsCI	GGATG	9 cut(s)	156, 171, 216, 540, 622, 858, 878, 937, 988
BtsI	GCAGTG	2 cut(s)	235, 2067
BtsIMutI	CAGTG	6 cut(s)	235, 254, 404, 1046, 1313, 2067
BveI	ACCTGC	1 cut(s)	298
Cac8I	GCNNGC	3 cut(s)	344, 1759, 1897
CaiI	CAGNNNCTG	1 cut(s)	1985
CfoI	GCGC	1 cut(s)	48
Cfr13I	GGNCC	4 cut(s)	403, 408, 1669, 2076
CseI	GACGC	2 cut(s)	37, 1571
Csp6I	GTAC	3 cut(s)	466, 1237, 1726
CviAII	CATG	9 cut(s)	670, 905, 1169, 1217, 1406, 1666, 1742, 1965, 2015
CviQI	GTAC	3 cut(s)	466, 1237, 1726
DdeI	CTNAG	2 cut(s)	645, 1996
DpnI	GATC	8 cut(s)	266, 358, 479, 584, 1109, 1290, 1371, 1616
DpnII	GATC	8 cut(s)	264, 356, 477, 582, 1107, 1288, 1369, 1614
DraI	TTTAAA	2 cut(s)	1263, 1905
Eam1104I	CTCTTC	2 cut(s)	375, 851
EarI	CTCTTC	2 cut(s)	375, 851
EciI	GGCGGA	4 cut(s)	97, 152, 953, 1286
Eco130I	CCWWGG	1 cut(s)	726
Eco24I	GRGCYC	2 cut(s)	348, 1897
Eco47I	GGWCC	1 cut(s)	403
Eco57I	CTGAAG	1 cut(s)	1788
Eco88I	CYCGRG	1 cut(s)	242
EcoNI	CCTNNNNNAGG	2 cut(s)	648, 1844
EcoRI	GAATTC	1 cut(s)	1399
EcoRII	CCWGG	1 cut(s)	515
EcoT14I	CCWWGG	1 cut(s)	726
EcoT22I	ATGCAT	2 cut(s)	622, 1170
EcoT38I	GRGCYC	2 cut(s)	348, 1897
ErhI	CCWWGG	1 cut(s)	726
FaeI	CATG	9 cut(s)	673, 908, 1172, 1220, 1409, 1669, 1745, 1968, 2018
FaqI	GGGAC	3 cut(s)	22, 416, 433
FatI	CATG	9 cut(s)	669, 904, 1168, 1216, 1405, 1665, 1741, 1964, 2014
FauI	CCCGC	2 cut(s)	809, 913
FauNDI	CATATG	1 cut(s)	1567
FbaI	TGATCA	6 cut(s)	264, 477, 582, 1107, 1369, 1614
FblI	GTMKAC	1 cut(s)	2067
Fnu4HI	GCNGC	3 cut(s)	166, 1356, 1781
FokI	GGATG	9 cut(s)	143, 158, 203, 547, 629, 865, 885, 944, 995
FriOI	GRGCYC	2 cut(s)	348, 1897
Fsp4HI	GCNGC	3 cut(s)	166, 1356, 1781
FspBI	CTAG	2 cut(s)	1346, 1607
GlaI	GCGC	1 cut(s)	47
GluI	GCNGC	3 cut(s)	166, 1356, 1781
GsaI	CCCAGC	1 cut(s)	614
GsuI	CTGGAG	1 cut(s)	1610
HaeIII	GGCC	5 cut(s)	410, 515, 725, 1670, 2078
HapII	CCGG	3 cut(s)	348, 406, 980
HgaI	GACGC	2 cut(s)	37, 1571
HhaI	GCGC	1 cut(s)	48
Hin1I	GRCGYC	1 cut(s)	144
Hin1II	CATG	9 cut(s)	673, 908, 1172, 1220, 1409, 1669, 1745, 1968, 2018
Hin6I	GCGC	1 cut(s)	46
HinP1I	GCGC	1 cut(s)	46
HincII	GTYRAC	1 cut(s)	2068
HindII	GTYRAC	1 cut(s)	2068
HindIII	AAGCTT	1 cut(s)	2004
HpaII	CCGG	3 cut(s)	348, 406, 980
HphI	GGTGA	3 cut(s)	760, 839, 1072
Hpy166II	GTNNAC	3 cut(s)	885, 1485, 2068
Hpy188III	TCNNGA	5 cut(s)	25, 481, 508, 662, 1292
Hpy8I	GTNNAC	3 cut(s)	885, 1485, 2068
Hpy99I	CGWCG	5 cut(s)	146, 191, 200, 212, 638
HpyAV	CCTTC	9 cut(s)	391, 505, 538, 961, 1063, 1126, 1168, 1331, 1763
HpyCH4III	ACNGT	6 cut(s)	262, 439, 1041, 1224, 1811, 1871
HpyCH4IV	ACGT	6 cut(s)	144, 195, 249, 464, 1199, 2039
HpyF10VI	GCNNNNNNNGC	7 cut(s)	348, 1142, 1184, 1277, 1758, 1763, 1772
HpyF3I	CTNAG	2 cut(s)	645, 1996
HpySE526I	ACGT	6 cut(s)	144, 195, 249, 464, 1199, 2039
Hsp92I	GRCGYC	1 cut(s)	144
Hsp92II	CATG	9 cut(s)	673, 908, 1172, 1220, 1409, 1669, 1745, 1968, 2018
HspAI	GCGC	1 cut(s)	46
Ksp22I	TGATCA	6 cut(s)	264, 477, 582, 1107, 1369, 1614
Kzo9I	GATC	8 cut(s)	264, 356, 477, 582, 1107, 1288, 1369, 1614
LmnI	GCTCC	2 cut(s)	63, 553
Lsp1109I	GCAGC	2 cut(s)	1367, 1792
LweI	GCATC	5 cut(s)	607, 1447, 1889, 1972, 2020
MaeI	CTAG	2 cut(s)	1346, 1607
MaeII	ACGT	6 cut(s)	144, 195, 249, 464, 1199, 2039
MaeIII	GTNAC	4 cut(s)	569, 1559, 1717, 1825
MalI	GATC	8 cut(s)	266, 358, 479, 584, 1109, 1290, 1371, 1616
MboI	GATC	8 cut(s)	264, 356, 477, 582, 1107, 1288, 1369, 1614
MhlI	GDGCHC	3 cut(s)	348, 1897, 1995
MlyI	GAGTC	5 cut(s)	98, 398, 663, 749, 1215
MmeI	TCCRAC	2 cut(s)	417, 2051
Mph1103I	ATGCAT	2 cut(s)	622, 1170
MroXI	GAANNNNTTC	1 cut(s)	503
MseI	TTAA	7 cut(s)	1163, 1211, 1262, 1380, 1631, 1904, 2101
MslI	CAYNNNNRTG	1 cut(s)	1841
MspI	CCGG	3 cut(s)	348, 406, 980
MspR9I	CCNGG	3 cut(s)	348, 407, 517
Mva1269I	GAATGC	1 cut(s)	351
MvaI	CCWGG	1 cut(s)	517
MvnI	CGCG	1 cut(s)	48
MwoI	GCNNNNNNNGC	7 cut(s)	348, 1142, 1184, 1277, 1758, 1763, 1772
NciI	CCSGG	2 cut(s)	348, 407
NdeI	CATATG	1 cut(s)	1567
NdeII	GATC	8 cut(s)	264, 356, 477, 582, 1107, 1288, 1369, 1614
NlaIII	CATG	9 cut(s)	673, 908, 1172, 1220, 1409, 1669, 1745, 1968, 2018
NlaIV	GGNNCC	3 cut(s)	404, 555, 2077
NmeAIII	GCCGAG	2 cut(s)	436, 1046
NmuCI	GTSAC	2 cut(s)	1559, 1825
NsiI	ATGCAT	2 cut(s)	622, 1170
NspI	RCATGY	1 cut(s)	1172
NspV	TTCGAA	2 cut(s)	432, 1707
PcsI	WCGNNNNNNNCGW	3 cut(s)	99, 192, 204
PctI	GAATGC	1 cut(s)	351
PdmI	GAANNNNTTC	1 cut(s)	503
PfeI	GAWTC	6 cut(s)	901, 1190, 1684, 1704, 1738, 1819
PflMI	CCANNNNNTGG	1 cut(s)	399
PkrI	GCNGC	3 cut(s)	167, 1357, 1782
PleI	GAGTC	5 cut(s)	98, 397, 662, 749, 1214
PpsI	GAGTC	5 cut(s)	98, 397, 662, 749, 1214
PshBI	ATTAAT	1 cut(s)	1163
PsiI	TTATAA	4 cut(s)	678, 786, 951, 1098
Psp6I	CCWGG	1 cut(s)	515
PspFI	CCCAGC	1 cut(s)	610
PspGI	CCWGG	1 cut(s)	515
PspN4I	GGNNCC	3 cut(s)	404, 555, 2077
PspPI	GGNCC	4 cut(s)	403, 408, 1669, 2076
PstI	CTGCAG	2 cut(s)	309, 1759
PstNI	CAGNNNCTG	1 cut(s)	1985
RsaI	GTAC	3 cut(s)	467, 1238, 1727
RsaNI	GTAC	3 cut(s)	466, 1237, 1726
RseI	CAYNNNNRTG	1 cut(s)	1841
SalI	GTCGAC	1 cut(s)	2066
SaqAI	TTAA	7 cut(s)	1163, 1211, 1262, 1380, 1631, 1904, 2101
SatI	GCNGC	3 cut(s)	166, 1356, 1781
Sau3AI	GATC	8 cut(s)	264, 356, 477, 582, 1107, 1288, 1369, 1614
Sau96I	GGNCC	4 cut(s)	403, 408, 1669, 2076
SbfI	CCTGCAGG	1 cut(s)	309
SchI	GAGTC	5 cut(s)	98, 398, 663, 749, 1215
ScrFI	CCNGG	3 cut(s)	348, 407, 517
SdaI	CCTGCAGG	1 cut(s)	309
SduI	GDGCHC	3 cut(s)	348, 1897, 1995
SfaNI	GCATC	5 cut(s)	607, 1447, 1889, 1972, 2020
SfcI	CTRYAG	2 cut(s)	305, 1755
SfuI	TTCGAA	2 cut(s)	432, 1707
SinI	GGWCC	1 cut(s)	403
SmiMI	CAYNNNNRTG	1 cut(s)	1841
SmlI	CTYRAG	1 cut(s)	53
SmoI	CTYRAG	1 cut(s)	53
Sse8387I	CCTGCAGG	1 cut(s)	309
SsiI	CCGC	9 cut(s)	87, 108, 163, 166, 220, 816, 920, 938, 1271
SspI	AATATT	1 cut(s)	1942
SspMI	CTAG	2 cut(s)	1346, 1607
StyD4I	CCNGG	3 cut(s)	346, 405, 515
StyI	CCWWGG	1 cut(s)	726
TaaI	ACNGT	6 cut(s)	262, 439, 1041, 1224, 1811, 1871
TaiI	ACGT	6 cut(s)	147, 198, 252, 467, 1202, 2042
TaqI	TCGA	9 cut(s)	102, 210, 355, 432, 507, 633, 1008, 1707, 2067
TaqII	GACCGA	1 cut(s)	188
TatI	WGTACW	1 cut(s)	1725
TauI	GCSGC	1 cut(s)	168
TfiI	GAWTC	6 cut(s)	901, 1190, 1684, 1704, 1738, 1819
Tru1I	TTAA	7 cut(s)	1163, 1211, 1262, 1380, 1631, 1904, 2101
Tru9I	TTAA	7 cut(s)	1163, 1211, 1262, 1380, 1631, 1904, 2101
TscAI	CASTG	6 cut(s)	242, 261, 404, 1046, 1320, 2067
TseFI	GTSAC	2 cut(s)	1559, 1825
TseI	GCWGC	2 cut(s)	1355, 1780
Tsp45I	GTSAC	2 cut(s)	1559, 1825
TspDTI	ATGAA	9 cut(s)	373, 869, 879, 893, 1205, 1365, 1407, 1730, 1953
TspRI	CASTG	6 cut(s)	242, 261, 404, 1046, 1320, 2067
Van91I	CCANNNNNTGG	1 cut(s)	399
VpaK11BI	GGWCC	1 cut(s)	403
VspI	ATTAAT	1 cut(s)	1163
XagI	CCTNNNNNAGG	2 cut(s)	648, 1844
XapI	RAATTY	3 cut(s)	454, 1399, 1887
XceI	RCATGY	1 cut(s)	1172
XcmI	CCANNNNNNNNNTGG	1 cut(s)	523
XmiI	GTMKAC	1 cut(s)	2067
XmnI	GAANNNNTTC	1 cut(s)	503
XspI	CTAG	2 cut(s)	1346, 1607
ZraI	GACGTC	1 cut(s)	145
Zsp2I	ATGCAT	2 cut(s)	622, 1170

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.