Rmu_sc0023851.1_g000002

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0023851.1
OG0000187
Physical Location & Seq
Forward (+)
803 .. 2151
1349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0023851.1_g000002.1.cds

Sequence Viewer

Length: 561 bp
ctgcctaaccggccatcctacatacgcgacctaaattcctatctgaaccgctcgggagcgagtggttcgatctcaacagcggcccaatcttggccaccaccatccttgccctcagagagaagcatcaccacgaaggccagatcctgtcctgctgcacgcgccatgcgccaaggaggccagaagcccgcgccgaagaggtactggatcactctccaaggtacctttgaacaggagatcgataagaatggcaagaaagttgggaagctttggattcgagtcaagatacctttgaacaagccattgccttttaaacgttggtactgggtagaagatgaggttgaattcttgtgcagattcaagtacgacaaacttttaggtcattgttctaattgcggcctggtgactcatgttggcttgtcgtgttcgggaccgacactgaacgaggatggtgaggaggttgagcgaactattctgggttcccagcctatggtactggcggtcgtagactggagtgctgccattgaacgaaaccacgacggtgtcgagctctggtacctctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

20.97

Weight (kDa)

9.13

Isoelectric Point (pI)

53.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000187)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20170 FvH4_4g06831
rosa_chinensis RchiOBHm_Chr1g0321991 RchiOBHm_Chr2g0161811
rosa_laevigata RLG00000015375 RLG00000018377
rosa_multiflora Rmu_co8011754.1_g000001 Rmu_co8019986.1_g000001 Rmu_co8067766.1_g000001 Rmu_co8156128.1_g000001 Rmu_co8219154.1_g000003 Rmu_co8297235.1_g000001 Rmu_co8345223.1_g000001 Rmu_co8364775.1_g000001 Rmu_co8465075.1_g000001 Rmu_sc0000007.1_g000002 Rmu_sc0000054.1_g000015 Rmu_sc0000054.1_g000023 Rmu_sc0000145.1_g000010 Rmu_sc0000166.1_g000006 Rmu_sc0000218.1_g000012 Rmu_sc0000339.1_g000008 Rmu_sc0000342.1_g000015 Rmu_sc0000464.1_g000008 Rmu_sc0000620.1_g000003 Rmu_sc0000638.1_g000017 Rmu_sc0000890.1_g000007 Rmu_sc0000894.1_g000024 Rmu_sc0000909.1_g000010 Rmu_sc0000928.1_g000020 Rmu_sc0000977.1_g000007 Rmu_sc0001022.1_g000003 Rmu_sc0001035.1_g000037 Rmu_sc0001060.1_g000008 Rmu_sc0001168.1_g000005 Rmu_sc0001173.1_g000022 Rmu_sc0001179.1_g000016 Rmu_sc0001408.1_g000003 Rmu_sc0001455.1_g000005 Rmu_sc0001484.1_g000008 Rmu_sc0001585.1_g000010 Rmu_sc0001702.1_g000030 Rmu_sc0001755.1_g000063 Rmu_sc0001903.1_g000009 Rmu_sc0002099.1_g000001 Rmu_sc0002142.1_g000012 Rmu_sc0002158.1_g000014 Rmu_sc0002307.1_g000009 Rmu_sc0002358.1_g000013 Rmu_sc0002421.1_g000002 Rmu_sc0002426.1_g000003 Rmu_sc0002438.1_g000001 Rmu_sc0002461.1_g000001 Rmu_sc0002508.1_g000003 Rmu_sc0002532.1_g000056 Rmu_sc0002583.1_g000002 Rmu_sc0002927.1_g000033 Rmu_sc0003033.1_g000040 Rmu_sc0003271.1_g000006 Rmu_sc0003381.1_g000006 Rmu_sc0003507.1_g000007 Rmu_sc0003578.1_g000003 Rmu_sc0003693.1_g000004 Rmu_sc0003693.1_g000005 Rmu_sc0003704.1_g000003 Rmu_sc0003840.1_g000002 Rmu_sc0004084.1_g000026 Rmu_sc0004193.1_g000007 Rmu_sc0004234.1_g000033 Rmu_sc0004235.1_g000025 Rmu_sc0004435.1_g000006 Rmu_sc0004727.1_g000003 Rmu_sc0004741.1_g000001 Rmu_sc0004926.1_g000004 Rmu_sc0005365.1_g000001 Rmu_sc0005392.1_g000007 Rmu_sc0005415.1_g000001 Rmu_sc0005539.1_g000007 Rmu_sc0005708.1_g000017 Rmu_sc0006317.1_g000007 Rmu_sc0006453.1_g000054 Rmu_sc0006536.1_g000004 Rmu_sc0006740.1_g000022 Rmu_sc0006763.1_g000012 Rmu_sc0007115.1_g000002 Rmu_sc0007115.1_g000010 Rmu_sc0007129.1_g000017 Rmu_sc0007131.1_g000010 Rmu_sc0008373.1_g000008 Rmu_sc0009005.1_g000008 Rmu_sc0009252.1_g000007 Rmu_sc0009599.1_g000022 Rmu_sc0009785.1_g000027 Rmu_sc0009891.1_g000003 Rmu_sc0010099.1_g000010 Rmu_sc0010266.1_g000008 Rmu_sc0010334.1_g000003 Rmu_sc0010381.1_g000001 Rmu_sc0010496.1_g000003 Rmu_sc0010496.1_g000004 Rmu_sc0010546.1_g000003 Rmu_sc0011482.1_g000006 Rmu_sc0011875.1_g000010 Rmu_sc0011917.1_g000006 Rmu_sc0012293.1_g000004 Rmu_sc0012847.1_g000003 Rmu_sc0013773.1_g000001 Rmu_sc0016060.1_g000003 Rmu_sc0016170.1_g000013 Rmu_sc0016393.1_g000002 Rmu_sc0017589.1_g000001 Rmu_sc0017786.1_g000005 Rmu_sc0018326.1_g000002 Rmu_sc0020760.1_g000001 Rmu_sc0021262.1_g000003 Rmu_sc0023851.1_g000002 Rmu_sc0024044.1_g000001 Rmu_sc0026820.1_g000001 Rmu_sc0032306.1_g000001 Rmu_sc0035666.1_g000001 Rmu_sc0036881.1_g000001 Rmu_sc0037030.1_g000001 Rmu_sc0037158.1_g000001 Rmu_ssc0000016.1_g000002 Rmu_ssc0000044.1_g000012 Rmu_ssc0000044.1_g000014 Rmu_ssc0000050.1_g000064 Rmu_ssc0000064.1_g000022 Rmu_ssc0000154.1_g000037 Rmu_ssc0000178.1_g000013 Rmu_ssc0000217.1_g000022 Rmu_ssc0000392.1_g000004
rosa_roxburghii Rroxscaffold_3G00242000 Rroxscaffold_3G00242530 Rroxscaffold_6G00406010
rosa_rugosa Rorug01G0062000 Rorug02G0224000 Rorug02G0262900 Rorug02G0263000 Rorug02G0358900 Rorug02G0360500 Rorug02G0378500.1 Rorug02G0378600 Rorug02G0394700 Rorug03G0122900 Rorug03G0254600 Rorug03G0254700 Rorug03G0254800 Rorug04G0042200.1 Rorug04G0378100 Rorug05G0053400 Rorug05G0238600 Rorug05G0364500 Rorug05G0562000 Rorug06G0089300 Rorug06G0089400 Rorug06G0145700 Rorug07G0111600 Rorug07G0229500.1 Rorug07G0287600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 218, 552
AccB1I GGYRCC 2 cut(s) 218, 552
AccB7I CCANNNNNTGG 1 cut(s) 487
AccBSI CCGCTC 1 cut(s) 51
AccI GTMKAC 1 cut(s) 504
AccII CGCG 3 cut(s) 27, 159, 188
AciI CCGC 5 cut(s) 49, 80, 186, 393, 497
AclI AACGTT 1 cut(s) 313
AclWI GGATC 2 cut(s) 135, 212
AcoI YGGCCR 2 cut(s) 11, 92
AcsI RAATTY 2 cut(s) 34, 341
AfaI GTAC 6 cut(s) 200, 220, 320, 362, 492, 554
AfiI CCNNNNNNNGG 2 cut(s) 90, 487
AgsI TTSAA 5 cut(s) 227, 292, 341, 358, 524
AjnI CCWGG 1 cut(s) 396
AleI CACNNNNGTG 1 cut(s) 537
AluBI AGCT 2 cut(s) 265, 547
AluI AGCT 2 cut(s) 265, 547
Alw21I GWGCWC 1 cut(s) 549
AlwI GGATC 2 cut(s) 135, 212
AlwNI CAGNNNCTG 1 cut(s) 144
Ama87I CYCGRG 1 cut(s) 52
AoxI GGCC 6 cut(s) 11, 81, 92, 135, 175, 394
ApeKI GCWGC 2 cut(s) 152, 515
ApoI RAATTY 2 cut(s) 34, 341
Asp718I GGTACC 2 cut(s) 218, 552
AspLEI GCGC 3 cut(s) 161, 168, 190
AspS9I GGNCC 2 cut(s) 82, 428
AsuHPI GGTGA 3 cut(s) 118, 412, 461
AvaI CYCGRG 1 cut(s) 52
AvaII GGWCC 1 cut(s) 428
BalI TGGCCA 1 cut(s) 94
BanI GGYRCC 2 cut(s) 218, 552
BanII GRGCYC 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 2 cut(s) 139, 502
BccI CCATC 3 cut(s) 22, 109, 440
BciT130I CCWGG 1 cut(s) 398
BfaI CTAG 1 cut(s) 559
BglI GCCNNNNNGGC 2 cut(s) 10, 174
BisI GCNGC 4 cut(s) 81, 153, 394, 516
BlsI GCNGC 4 cut(s) 82, 154, 395, 517
Bme1390I CCNGG 1 cut(s) 398
Bme18I GGWCC 1 cut(s) 428
BmeT110I CYCGRG 1 cut(s) 52
BmgT120I GGNCC 2 cut(s) 82, 428
BmiI GGNNCC 4 cut(s) 220, 429, 478, 554
BmrFI CCNGG 1 cut(s) 398
BmrI ACTGGG 1 cut(s) 331
BmsI GCATC 1 cut(s) 132
BmuI ACTGGG 1 cut(s) 331
BpmI CTGGAG 1 cut(s) 529
Bsa29I ATCGAT 1 cut(s) 237
BsaJI CCNNGG 2 cut(s) 169, 214
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 487
Bse118I RCCGGY 1 cut(s) 9
Bse1I ACTGG 4 cut(s) 206, 326, 498, 512
Bse3DI GCAATG 1 cut(s) 299
BseBI CCWGG 1 cut(s) 398
BseCI ATCGAT 1 cut(s) 237
BseDI CCNNGG 2 cut(s) 169, 214
BseGI GGATG 3 cut(s) 14, 101, 451
BseLI CCNNNNNNNGG 2 cut(s) 90, 487
BseMI GCAATG 1 cut(s) 299
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 4 cut(s) 206, 326, 498, 512
BseRI GAGGAG 1 cut(s) 467
BseXI GCAGC 2 cut(s) 139, 502
BseYI CCCAGC 1 cut(s) 480
BsgI GTGCAG 2 cut(s) 138, 370
Bsh1236I CGCG 3 cut(s) 27, 159, 188
Bsh1285I CGRYCG 1 cut(s) 501
BshFI GGCC 6 cut(s) 13, 83, 94, 137, 177, 396
BshNI GGYRCC 2 cut(s) 218, 552
BshVI ATCGAT 1 cut(s) 237
BsiEI CGRYCG 1 cut(s) 501
BsiHKAI GWGCWC 1 cut(s) 549
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 1 cut(s) 10
BslFI GGGAC 1 cut(s) 441
BslI CCNNNNNNNGG 2 cut(s) 90, 487
BsmFI GGGAC 1 cut(s) 441
BsnI GGCC 6 cut(s) 13, 83, 94, 137, 177, 396
BsoBI CYCGRG 1 cut(s) 52
Bsp1286I GDGCHC 1 cut(s) 549
Bsp143I GATC 4 cut(s) 69, 140, 204, 234
BspACI CCGC 5 cut(s) 49, 80, 186, 393, 497
BspANI GGCC 6 cut(s) 13, 83, 94, 137, 177, 396
BspCNI CTCAG 1 cut(s) 125
BspDI ATCGAT 1 cut(s) 237
BspFNI CGCG 3 cut(s) 27, 159, 188
BspLI GGNNCC 4 cut(s) 220, 429, 478, 554
BspPI GGATC 2 cut(s) 135, 212
BspT107I GGYRCC 2 cut(s) 218, 552
BsrBI CCGCTC 1 cut(s) 51
BsrDI GCAATG 1 cut(s) 299
BsrFI RCCGGY 1 cut(s) 9
BsrI ACTGG 4 cut(s) 206, 326, 498, 512
BssAI RCCGGY 1 cut(s) 9
BssECI CCNNGG 2 cut(s) 169, 214
BssMI GATC 4 cut(s) 69, 140, 204, 234
BssT1I CCWWGG 2 cut(s) 169, 214
Bst2UI CCWGG 1 cut(s) 398
Bst4CI ACNGT 1 cut(s) 539
Bst6I CTCTTC 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 157, 186
BstDEI CTNAG 1 cut(s) 112
BstF5I GGATG 3 cut(s) 14, 101, 451
BstFNI CGCG 3 cut(s) 27, 159, 188
BstHHI GCGC 3 cut(s) 161, 168, 190
BstKTI GATC 4 cut(s) 72, 143, 207, 237
BstMBI GATC 4 cut(s) 69, 140, 204, 234
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 4 cut(s) 10, 158, 165, 174
BstNI CCWGG 1 cut(s) 398
BstSCI CCNGG 1 cut(s) 396
BstUI CGCG 3 cut(s) 27, 159, 188
BstV1I GCAGC 2 cut(s) 139, 502
BstX2I RGATCY 1 cut(s) 140
BstYI RGATCY 1 cut(s) 140
Bsu15I ATCGAT 1 cut(s) 237
BsuRI GGCC 6 cut(s) 13, 83, 94, 137, 177, 396
BsuTUI ATCGAT 1 cut(s) 237
BtsCI GGATG 3 cut(s) 14, 101, 451
BtsIMutI CAGTG 1 cut(s) 434
Cac8I GCNNGC 2 cut(s) 157, 186
CaiI CAGNNNCTG 1 cut(s) 144
CfoI GCGC 3 cut(s) 161, 168, 190
Cfr10I RCCGGY 1 cut(s) 9
Cfr13I GGNCC 2 cut(s) 82, 428
ClaI ATCGAT 1 cut(s) 237
Csp6I GTAC 6 cut(s) 199, 219, 319, 361, 491, 553
CspCI CAANNNNNGTGG 2 cut(s) 87, 122
CviAII CATG 2 cut(s) 163, 407
CviQI GTAC 6 cut(s) 199, 219, 319, 361, 491, 553
DdeI CTNAG 1 cut(s) 112
DpnI GATC 4 cut(s) 71, 142, 206, 236
DpnII GATC 4 cut(s) 69, 140, 204, 234
DraI TTTAAA 1 cut(s) 310
EaeI YGGCCR 2 cut(s) 11, 92
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
Ecl136II GAGCTC 1 cut(s) 547
Eco130I CCWWGG 2 cut(s) 169, 214
Eco24I GRGCYC 1 cut(s) 549
Eco47I GGWCC 1 cut(s) 428
Eco53kI GAGCTC 1 cut(s) 547
Eco88I CYCGRG 1 cut(s) 52
EcoICRI GAGCTC 1 cut(s) 547
EcoRI GAATTC 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 396
EcoT14I CCWWGG 2 cut(s) 169, 214
EcoT38I GRGCYC 1 cut(s) 549
ErhI CCWWGG 2 cut(s) 169, 214
FaeI CATG 2 cut(s) 166, 410
FaiI YATR 4 cut(s) 23, 164, 408, 488
FaqI GGGAC 1 cut(s) 441
FatI CATG 2 cut(s) 162, 406
FauI CCCGC 1 cut(s) 193
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 4 cut(s) 81, 153, 394, 516
FokI GGATG 2 cut(s) 88, 458
FriOI GRGCYC 1 cut(s) 549
Fsp4HI GCNGC 4 cut(s) 81, 153, 394, 516
FspBI CTAG 1 cut(s) 559
GlaI GCGC 3 cut(s) 160, 167, 189
GluI GCNGC 4 cut(s) 81, 153, 394, 516
GsaI CCCAGC 1 cut(s) 484
GsuI CTGGAG 1 cut(s) 529
HaeIII GGCC 6 cut(s) 13, 83, 94, 137, 177, 396
HapII CCGG 1 cut(s) 10
HhaI GCGC 3 cut(s) 161, 168, 190
Hin1II CATG 2 cut(s) 166, 410
Hin6I GCGC 3 cut(s) 159, 166, 188
HinP1I GCGC 3 cut(s) 159, 166, 188
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 4 cut(s) 271, 276, 354, 403
HpaII CCGG 1 cut(s) 10
HphI GGTGA 3 cut(s) 118, 412, 461
Hpy166II GTNNAC 1 cut(s) 505
Hpy188I TCNGA 2 cut(s) 45, 115
Hpy188III TCNNGA 3 cut(s) 54, 280, 426
Hpy8I GTNNAC 1 cut(s) 505
Hpy99I CGWCG 1 cut(s) 539
HpyAV CCTTC 1 cut(s) 127
HpyCH4III ACNGT 1 cut(s) 539
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 2 cut(s) 155, 351
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 158, 165, 174
HpyF3I CTNAG 1 cut(s) 112
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 2 cut(s) 166, 410
HspAI GCGC 3 cut(s) 159, 166, 188
KpnI GGTACC 2 cut(s) 222, 556
Kzo9I GATC 4 cut(s) 69, 140, 204, 234
LmnI GCTCC 1 cut(s) 56
Lsp1109I GCAGC 2 cut(s) 139, 502
LweI GCATC 1 cut(s) 132
MaeI CTAG 1 cut(s) 559
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 1 cut(s) 400
MalI GATC 4 cut(s) 71, 142, 206, 236
MbiI CCGCTC 1 cut(s) 51
MboI GATC 4 cut(s) 69, 140, 204, 234
MboII GAAGA 2 cut(s) 205, 341
MflI RGATCY 1 cut(s) 140
MhlI GDGCHC 1 cut(s) 549
MlsI TGGCCA 1 cut(s) 94
MluCI AATT 3 cut(s) 34, 341, 388
MluNI TGGCCA 1 cut(s) 94
MlyI GAGTC 2 cut(s) 285, 397
MnlI CCTC 7 cut(s) 121, 167, 189, 328, 436, 445, 448
Mox20I TGGCCA 1 cut(s) 94
MscI TGGCCA 1 cut(s) 94
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 537
Msp20I TGGCCA 1 cut(s) 94
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 1 cut(s) 10
MspR9I CCNGG 1 cut(s) 398
MvaI CCWGG 1 cut(s) 398
MvnI CGCG 3 cut(s) 27, 159, 188
MwoI GCNNNNNNNGC 4 cut(s) 10, 158, 165, 174
NdeII GATC 4 cut(s) 69, 140, 204, 234
NlaIII CATG 2 cut(s) 166, 410
NlaIV GGNNCC 4 cut(s) 220, 429, 478, 554
NmuCI GTSAC 1 cut(s) 400
OliI CACNNNNGTG 1 cut(s) 537
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PfeI GAWTC 2 cut(s) 271, 354
PflFI GACNNNGTC 1 cut(s) 539
PflMI CCANNNNNTGG 1 cut(s) 487
PkrI GCNGC 4 cut(s) 82, 154, 395, 517
PleI GAGTC 2 cut(s) 284, 397
PpsI GAGTC 2 cut(s) 284, 397
Psp124BI GAGCTC 1 cut(s) 549
Psp1406I AACGTT 1 cut(s) 313
Psp6I CCWGG 1 cut(s) 396
PspFI CCCAGC 1 cut(s) 480
PspGI CCWGG 1 cut(s) 396
PspN4I GGNNCC 4 cut(s) 220, 429, 478, 554
PspPI GGNCC 2 cut(s) 82, 428
PstNI CAGNNNCTG 1 cut(s) 144
PsuI RGATCY 1 cut(s) 140
PsyI GACNNNGTC 1 cut(s) 539
RsaI GTAC 6 cut(s) 200, 220, 320, 362, 492, 554
RsaNI GTAC 6 cut(s) 199, 219, 319, 361, 491, 553
RseI CAYNNNNRTG 1 cut(s) 537
SacI GAGCTC 1 cut(s) 549
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 4 cut(s) 81, 153, 394, 516
Sau3AI GATC 4 cut(s) 69, 140, 204, 234
Sau96I GGNCC 2 cut(s) 82, 428
SchI GAGTC 2 cut(s) 285, 397
ScrFI CCNGG 1 cut(s) 398
SduI GDGCHC 1 cut(s) 549
SfaNI GCATC 1 cut(s) 132
SinI GGWCC 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 537
Sse9I AATT 3 cut(s) 34, 341, 388
SsiI CCGC 5 cut(s) 49, 80, 186, 393, 497
SspMI CTAG 1 cut(s) 559
SstI GAGCTC 1 cut(s) 549
StyD4I CCNGG 1 cut(s) 396
StyI CCWWGG 2 cut(s) 169, 214
TaaI ACNGT 1 cut(s) 539
TaiI ACGT 1 cut(s) 316
TaqI TCGA 4 cut(s) 68, 237, 274, 543
TaqII GACCGA 1 cut(s) 445
TasI AATT 3 cut(s) 34, 341, 388
TauI GCSGC 2 cut(s) 83, 396
TfiI GAWTC 2 cut(s) 271, 354
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 1 cut(s) 441
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 2 cut(s) 152, 515
Tsp45I GTSAC 1 cut(s) 400
TspRI CASTG 1 cut(s) 441
Tth111I GACNNNGTC 1 cut(s) 539
Van91I CCANNNNNTGG 1 cut(s) 487
VpaK11BI GGWCC 1 cut(s) 428
XapI RAATTY 2 cut(s) 34, 341
XmiI GTMKAC 1 cut(s) 504
XspI CTAG 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.