Rmu_co8345223.1_g000001

Zinc knuckle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8345223.1
OG0000187
Physical Location & Seq
Reverse (-)
2 .. 938
937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8345223.1_g000001.1.cds

Sequence Viewer

Length: 775 bp
ctggcttcttctcttgcaattgctgaggggaggaaacaggtcgatctgcgaccgacgcaaggaaaaggtttgcgacaggcagatgtgtttatgttggggaagctgctgactgctcgggagtacaagacccgctcttttatggggttgttccgcaatgcatggcgggtgaatggatcactaagggtggaggaagcggagggacagagggttctcatcactttttcggacgctactgaccgggatcggatctggaagggatctccatggggatataatcacgctccgattgccctaacttactatgatggtgtggcggccatggactcggtccctttcaccttatcctcatactggattacactgcaaggaatccctcttgcgttccgctcggagagggtgatgactatgattggttttacccttggtgatttcaaggagattgataaacaggccaagaaggttggaaagctccgaatcaaggtggatatccctttcaaccggcctctgtccttccaacgctggtattgggtagaggatgaggttgagttccactgcaagtttcagtttgataaactctttggtcgatgctcgatttgtgtctatgttactcacgtaggtctaccttgctcgggacctccattggaggagaatgatgaggcagaacgcacggttctggtgctgctacaacggctccgacgtccagctcatatcagagagctgcctgagatgatggctgtcccagaaagtgttatagggcagaggagacaaagggagg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

258

Amino Acids

29.7

Weight (kDa)

9.3

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000187)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20170 FvH4_4g06831
rosa_chinensis RchiOBHm_Chr1g0321991 RchiOBHm_Chr2g0161811
rosa_laevigata RLG00000015375 RLG00000018377
rosa_multiflora Rmu_co8011754.1_g000001 Rmu_co8019986.1_g000001 Rmu_co8067766.1_g000001 Rmu_co8156128.1_g000001 Rmu_co8219154.1_g000003 Rmu_co8297235.1_g000001 Rmu_co8345223.1_g000001 Rmu_co8364775.1_g000001 Rmu_co8465075.1_g000001 Rmu_sc0000007.1_g000002 Rmu_sc0000054.1_g000015 Rmu_sc0000054.1_g000023 Rmu_sc0000145.1_g000010 Rmu_sc0000166.1_g000006 Rmu_sc0000218.1_g000012 Rmu_sc0000339.1_g000008 Rmu_sc0000342.1_g000015 Rmu_sc0000464.1_g000008 Rmu_sc0000620.1_g000003 Rmu_sc0000638.1_g000017 Rmu_sc0000890.1_g000007 Rmu_sc0000894.1_g000024 Rmu_sc0000909.1_g000010 Rmu_sc0000928.1_g000020 Rmu_sc0000977.1_g000007 Rmu_sc0001022.1_g000003 Rmu_sc0001035.1_g000037 Rmu_sc0001060.1_g000008 Rmu_sc0001168.1_g000005 Rmu_sc0001173.1_g000022 Rmu_sc0001179.1_g000016 Rmu_sc0001408.1_g000003 Rmu_sc0001455.1_g000005 Rmu_sc0001484.1_g000008 Rmu_sc0001585.1_g000010 Rmu_sc0001702.1_g000030 Rmu_sc0001755.1_g000063 Rmu_sc0001903.1_g000009 Rmu_sc0002099.1_g000001 Rmu_sc0002142.1_g000012 Rmu_sc0002158.1_g000014 Rmu_sc0002307.1_g000009 Rmu_sc0002358.1_g000013 Rmu_sc0002421.1_g000002 Rmu_sc0002426.1_g000003 Rmu_sc0002438.1_g000001 Rmu_sc0002461.1_g000001 Rmu_sc0002508.1_g000003 Rmu_sc0002532.1_g000056 Rmu_sc0002583.1_g000002 Rmu_sc0002927.1_g000033 Rmu_sc0003033.1_g000040 Rmu_sc0003271.1_g000006 Rmu_sc0003381.1_g000006 Rmu_sc0003507.1_g000007 Rmu_sc0003578.1_g000003 Rmu_sc0003693.1_g000004 Rmu_sc0003693.1_g000005 Rmu_sc0003704.1_g000003 Rmu_sc0003840.1_g000002 Rmu_sc0004084.1_g000026 Rmu_sc0004193.1_g000007 Rmu_sc0004234.1_g000033 Rmu_sc0004235.1_g000025 Rmu_sc0004435.1_g000006 Rmu_sc0004727.1_g000003 Rmu_sc0004741.1_g000001 Rmu_sc0004926.1_g000004 Rmu_sc0005365.1_g000001 Rmu_sc0005392.1_g000007 Rmu_sc0005415.1_g000001 Rmu_sc0005539.1_g000007 Rmu_sc0005708.1_g000017 Rmu_sc0006317.1_g000007 Rmu_sc0006453.1_g000054 Rmu_sc0006536.1_g000004 Rmu_sc0006740.1_g000022 Rmu_sc0006763.1_g000012 Rmu_sc0007115.1_g000002 Rmu_sc0007115.1_g000010 Rmu_sc0007129.1_g000017 Rmu_sc0007131.1_g000010 Rmu_sc0008373.1_g000008 Rmu_sc0009005.1_g000008 Rmu_sc0009252.1_g000007 Rmu_sc0009599.1_g000022 Rmu_sc0009785.1_g000027 Rmu_sc0009891.1_g000003 Rmu_sc0010099.1_g000010 Rmu_sc0010266.1_g000008 Rmu_sc0010334.1_g000003 Rmu_sc0010381.1_g000001 Rmu_sc0010496.1_g000003 Rmu_sc0010496.1_g000004 Rmu_sc0010546.1_g000003 Rmu_sc0011482.1_g000006 Rmu_sc0011875.1_g000010 Rmu_sc0011917.1_g000006 Rmu_sc0012293.1_g000004 Rmu_sc0012847.1_g000003 Rmu_sc0013773.1_g000001 Rmu_sc0016060.1_g000003 Rmu_sc0016170.1_g000013 Rmu_sc0016393.1_g000002 Rmu_sc0017589.1_g000001 Rmu_sc0017786.1_g000005 Rmu_sc0018326.1_g000002 Rmu_sc0020760.1_g000001 Rmu_sc0021262.1_g000003 Rmu_sc0023851.1_g000002 Rmu_sc0024044.1_g000001 Rmu_sc0026820.1_g000001 Rmu_sc0032306.1_g000001 Rmu_sc0035666.1_g000001 Rmu_sc0036881.1_g000001 Rmu_sc0037030.1_g000001 Rmu_sc0037158.1_g000001 Rmu_ssc0000016.1_g000002 Rmu_ssc0000044.1_g000012 Rmu_ssc0000044.1_g000014 Rmu_ssc0000050.1_g000064 Rmu_ssc0000064.1_g000022 Rmu_ssc0000154.1_g000037 Rmu_ssc0000178.1_g000013 Rmu_ssc0000217.1_g000022 Rmu_ssc0000392.1_g000004
rosa_roxburghii Rroxscaffold_3G00242000 Rroxscaffold_3G00242530 Rroxscaffold_6G00406010
rosa_rugosa Rorug01G0062000 Rorug02G0224000 Rorug02G0262900 Rorug02G0263000 Rorug02G0358900 Rorug02G0360500 Rorug02G0378500.1 Rorug02G0378600 Rorug02G0394700 Rorug03G0122900 Rorug03G0254600 Rorug03G0254700 Rorug03G0254800 Rorug04G0042200.1 Rorug04G0378100 Rorug05G0053400 Rorug05G0238600 Rorug05G0364500 Rorug05G0562000 Rorug06G0089300 Rorug06G0089400 Rorug06G0145700 Rorug07G0111600 Rorug07G0229500.1 Rorug07G0287600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 700
AccBSI CCGCTC 2 cut(s) 132, 387
AccI GTMKAC 1 cut(s) 619
AciI CCGC 6 cut(s) 130, 151, 163, 194, 314, 385
AclWI GGATC 4 cut(s) 181, 249, 254, 265
AcoI YGGCCR 1 cut(s) 315
AcyI GRCGYC 1 cut(s) 697
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 4 cut(s) 59, 351, 478, 629
AgsI TTSAA 2 cut(s) 433, 496
AloI GAACNNNNNNTCC 2 cut(s) 192, 224
AluBI AGCT 4 cut(s) 103, 469, 704, 718
AluI AGCT 4 cut(s) 103, 469, 704, 718
Alw26I GTCTC 1 cut(s) 757
AlwI GGATC 4 cut(s) 181, 249, 254, 265
Ama87I CYCGRG 2 cut(s) 114, 628
AoxI GGCC 3 cut(s) 315, 450, 500
ApeKI GCWGC 3 cut(s) 103, 679, 718
AspS9I GGNCC 2 cut(s) 328, 632
AsuC2I CCSGG 1 cut(s) 239
AsuHPI GGTGA 4 cut(s) 178, 328, 409, 437
AvaI CYCGRG 2 cut(s) 114, 628
AvaII GGWCC 2 cut(s) 328, 632
BbvCI CCTCAGC 1 cut(s) 24
BbvI GCAGC 3 cut(s) 90, 666, 705
BccI CCATC 2 cut(s) 299, 724
BceAI ACGGC 1 cut(s) 704
BcnI CCSGG 1 cut(s) 239
BcoDI GTCTC 1 cut(s) 757
BisI GCNGC 4 cut(s) 104, 315, 680, 719
BlsI GCNGC 4 cut(s) 105, 316, 681, 720
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 2 cut(s) 328, 632
BmeT110I CYCGRG 2 cut(s) 114, 628
BmgT120I GGNCC 2 cut(s) 328, 632
BmiI GGNNCC 3 cut(s) 330, 633, 692
BmrFI CCNGG 1 cut(s) 239
BmsI GCATC 1 cut(s) 575
Bpu10I CCTNAGC 1 cut(s) 24
BpuMI CCSGG 1 cut(s) 239
BsaAI YACGTR 1 cut(s) 613
BsaHI GRCGYC 1 cut(s) 697
BsaJI CCNNGG 3 cut(s) 263, 318, 421
BsaXI ACNNNNNCTCC 2 cut(s) 675, 705
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 351, 478, 629
Bse118I RCCGGY 1 cut(s) 498
Bse1I ACTGG 1 cut(s) 356
Bse3DI GCAATG 1 cut(s) 160
BseDI CCNNGG 3 cut(s) 263, 318, 421
BseGI GGATG 1 cut(s) 541
BseLI CCNNNNNNNGG 4 cut(s) 59, 351, 478, 629
BseMI GCAATG 1 cut(s) 160
BseMII CTCAG 2 cut(s) 15, 714
BseNI ACTGG 1 cut(s) 356
BseRI GAGGAG 2 cut(s) 659, 775
BseXI GCAGC 3 cut(s) 90, 666, 705
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 3 cut(s) 317, 452, 502
BsiEI CGRYCG 1 cut(s) 53
BsiHKCI CYCGRG 2 cut(s) 114, 628
BsiSI CCGG 2 cut(s) 238, 499
BslFI GGGAC 4 cut(s) 213, 314, 645, 722
BslI CCNNNNNNNGG 4 cut(s) 59, 351, 478, 629
BsmAI GTCTC 1 cut(s) 757
BsmFI GGGAC 4 cut(s) 213, 314, 645, 722
BsnI GGCC 3 cut(s) 317, 452, 502
BsoBI CYCGRG 2 cut(s) 114, 628
Bsp143I GATC 5 cut(s) 43, 173, 241, 246, 257
Bsp19I CCATGG 2 cut(s) 263, 318
BspACI CCGC 6 cut(s) 130, 151, 163, 194, 314, 385
BspANI GGCC 3 cut(s) 317, 452, 502
BspCNI CTCAG 2 cut(s) 16, 715
BspLI GGNNCC 3 cut(s) 330, 633, 692
BspPI GGATC 4 cut(s) 181, 249, 254, 265
BsrBI CCGCTC 2 cut(s) 132, 387
BsrDI GCAATG 1 cut(s) 160
BsrFI RCCGGY 1 cut(s) 498
BsrI ACTGG 1 cut(s) 356
BssAI RCCGGY 1 cut(s) 498
BssECI CCNNGG 3 cut(s) 263, 318, 421
BssMI GATC 5 cut(s) 43, 173, 241, 246, 257
BssNI GRCGYC 1 cut(s) 697
BssT1I CCWWGG 3 cut(s) 263, 318, 421
Bst4CI ACNGT 1 cut(s) 670
BstACI GRCGYC 1 cut(s) 697
BstBAI YACGTR 1 cut(s) 613
BstDEI CTNAG 3 cut(s) 24, 179, 723
BstDSI CCRYGG 2 cut(s) 263, 318
BstF5I GGATG 1 cut(s) 541
BstKTI GATC 5 cut(s) 46, 176, 244, 249, 260
BstMAI GTCTC 1 cut(s) 757
BstMBI GATC 5 cut(s) 43, 173, 241, 246, 257
BstMCI CGRYCG 1 cut(s) 53
BstMWI GCNNNNNNNGC 3 cut(s) 55, 287, 688
BstSCI CCNGG 1 cut(s) 237
BstV1I GCAGC 3 cut(s) 90, 666, 705
BstX2I RGATCY 2 cut(s) 246, 257
BstYI RGATCY 2 cut(s) 246, 257
BsuRI GGCC 3 cut(s) 317, 452, 502
BtgI CCRYGG 2 cut(s) 263, 318
BtsCI GGATG 1 cut(s) 541
BtsI GCAGTG 2 cut(s) 359, 550
BtsIMutI CAGTG 2 cut(s) 359, 550
Cfr10I RCCGGY 1 cut(s) 498
Cfr13I GGNCC 2 cut(s) 328, 632
CseI GACGC 2 cut(s) 64, 236
Csp6I GTAC 1 cut(s) 121
CviAII CATG 3 cut(s) 159, 264, 319
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 3 cut(s) 24, 179, 723
DpnI GATC 5 cut(s) 45, 175, 243, 248, 259
DpnII GATC 5 cut(s) 43, 173, 241, 246, 257
EaeI YGGCCR 1 cut(s) 315
Eco130I CCWWGG 3 cut(s) 263, 318, 421
Eco32I GATATC 1 cut(s) 487
Eco47I GGWCC 2 cut(s) 328, 632
Eco88I CYCGRG 2 cut(s) 114, 628
EcoO109I RGGNCCY 1 cut(s) 632
EcoRV GATATC 1 cut(s) 487
EcoT14I CCWWGG 3 cut(s) 263, 318, 421
EcoT22I ATGCAT 1 cut(s) 160
ErhI CCWWGG 3 cut(s) 263, 318, 421
FaeI CATG 3 cut(s) 162, 267, 322
FaqI GGGAC 4 cut(s) 213, 314, 645, 722
FatI CATG 3 cut(s) 158, 263, 318
FauI CCCGC 2 cut(s) 137, 156
FblI GTMKAC 1 cut(s) 619
Fnu4HI GCNGC 4 cut(s) 104, 315, 680, 719
FokI GGATG 1 cut(s) 548
Fsp4HI GCNGC 4 cut(s) 104, 315, 680, 719
GluI GCNGC 4 cut(s) 104, 315, 680, 719
HaeIII GGCC 3 cut(s) 317, 452, 502
HapII CCGG 2 cut(s) 238, 499
HgaI GACGC 2 cut(s) 64, 236
Hin1I GRCGYC 1 cut(s) 697
Hin1II CATG 3 cut(s) 162, 267, 322
HinfI GANTC 3 cut(s) 323, 369, 474
HpaII CCGG 2 cut(s) 238, 499
HphI GGTGA 4 cut(s) 178, 328, 409, 437
Hpy166II GTNNAC 1 cut(s) 620
Hpy188I TCNGA 7 cut(s) 226, 246, 285, 391, 473, 695, 713
Hpy188III TCNNGA 3 cut(s) 116, 250, 630
Hpy8I GTNNAC 1 cut(s) 620
Hpy99I CGWCG 2 cut(s) 58, 699
HpyAV CCTTC 3 cut(s) 247, 451, 520
HpyCH4III ACNGT 1 cut(s) 670
HpyCH4IV ACGT 2 cut(s) 612, 697
HpyCH4V TGCA 4 cut(s) 17, 158, 364, 555
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 287, 688
HpyF3I CTNAG 3 cut(s) 24, 179, 723
HpySE526I ACGT 2 cut(s) 612, 697
Hsp92I GRCGYC 1 cut(s) 697
Hsp92II CATG 3 cut(s) 162, 267, 322
Kzo9I GATC 5 cut(s) 43, 173, 241, 246, 257
LmnI GCTCC 3 cut(s) 286, 474, 696
Lsp1109I GCAGC 3 cut(s) 90, 666, 705
LweI GCATC 1 cut(s) 575
MaeII ACGT 2 cut(s) 612, 697
MaeIII GTNAC 1 cut(s) 604
MalI GATC 5 cut(s) 45, 175, 243, 248, 259
MbiI CCGCTC 2 cut(s) 132, 387
MboI GATC 5 cut(s) 43, 173, 241, 246, 257
MfeI CAATTG 1 cut(s) 18
MflI RGATCY 2 cut(s) 246, 257
MluCI AATT 1 cut(s) 18
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 3 cut(s) 442, 538, 718
Mph1103I ATGCAT 1 cut(s) 160
MspI CCGG 2 cut(s) 238, 499
MspR9I CCNGG 1 cut(s) 239
MunI CAATTG 1 cut(s) 18
MwoI GCNNNNNNNGC 3 cut(s) 55, 287, 688
NciI CCSGG 1 cut(s) 239
NcoI CCATGG 2 cut(s) 263, 318
NdeII GATC 5 cut(s) 43, 173, 241, 246, 257
NlaIII CATG 3 cut(s) 162, 267, 322
NlaIV GGNNCC 3 cut(s) 330, 633, 692
NsiI ATGCAT 1 cut(s) 160
PcsI WCGNNNNNNNCGW 1 cut(s) 694
PfeI GAWTC 2 cut(s) 369, 474
PflFI GACNNNGTC 1 cut(s) 326
PkrI GCNGC 4 cut(s) 105, 316, 681, 720
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
Ppu21I YACGTR 1 cut(s) 613
PpuMI RGGWCCY 1 cut(s) 632
Psp5II RGGWCCY 1 cut(s) 632
PspN4I GGNNCC 3 cut(s) 330, 633, 692
PspPI GGNCC 2 cut(s) 328, 632
PspPPI RGGWCCY 1 cut(s) 632
PsuI RGATCY 2 cut(s) 246, 257
PsyI GACNNNGTC 1 cut(s) 326
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
SatI GCNGC 4 cut(s) 104, 315, 680, 719
Sau3AI GATC 5 cut(s) 43, 173, 241, 246, 257
Sau96I GGNCC 2 cut(s) 328, 632
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 239
SfaNI GCATC 1 cut(s) 575
SinI GGWCC 2 cut(s) 328, 632
Sse9I AATT 1 cut(s) 18
SsiI CCGC 6 cut(s) 130, 151, 163, 194, 314, 385
StyD4I CCNGG 1 cut(s) 237
StyI CCWWGG 3 cut(s) 263, 318, 421
TaaI ACNGT 1 cut(s) 670
TaiI ACGT 2 cut(s) 615, 700
TaqI TCGA 3 cut(s) 42, 583, 590
TaqII GACCGA 2 cut(s) 67, 316
TasI AATT 1 cut(s) 18
TatI WGTACW 1 cut(s) 120
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 2 cut(s) 369, 474
TscAI CASTG 2 cut(s) 366, 557
TseI GCWGC 3 cut(s) 103, 679, 718
TspRI CASTG 2 cut(s) 366, 557
Tth111I GACNNNGTC 1 cut(s) 326
VpaK11BI GGWCC 2 cut(s) 328, 632
XmiI GTMKAC 1 cut(s) 619
ZraI GACGTC 1 cut(s) 698
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.