Rorug07G0287600

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000187
Physical Location & Seq
Reverse (-)
27977547 .. 27980496
2950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0287600.1

Sequence Viewer

Length: 2475 bp
ATGAAGAAATCTCACTTCCACTCCTCACCCCTCCCTTCACTCCGTCGCTTCTACCTCTTAAACCCCAAGCCCCTCTCCTCACAACCACCACCACCGCCGCCGAACCCAATTCCACAACCCTCCGATTCAACCCAAACCCAACTCCTAAACACTCTTCTTTCCCACAAGACCGATCCCAGTTCCGCTCTTGACCTCCTCAAACGCGCCGAAGCTCACCCAGGCTTTGTCAACGGCGTCGACACCGTCGCCGTCTTGCTTCACATCTTAATGGGCTTTCCAGAGACTCGCGGTCGCGCCAAGACATTGCTTAACCGCTACGTTTCCGGCGACTCCGGCCCTCCTCCTCCTCCTCCGGCAGTCTTTCTCGATCGTTTGCTGGACTGCGCAAAGAGGTTTGGTTTTGATTTAGATTCTTGGGTTTTCAGTTACTTGTTGAATAGCTATGTTAGAGCCAATCGAATAGGGGATGCTGTTGATTGCTTTAATAGAATGGTGGAGCTTGAGATATATGCTGGTGGTGTTGAATATATGAACATTGTGTTGACGGCATTGGTTCGGAGGAATATGTTTGAGGAAGCACGAGAGTTGTATGGTAAAATGGTTGACACAGGAGTTGGCGGTGATTGCGCCACTCTACATGTCATGATGCGTGCTTGTTTGAAGGAGGGGAAGCCGGAGGAGGCCGAGGAGTACTTTCGGAAGGCAAGGGGGAGAGGCATAGCAGCTGATGCCGCATTGTATGATGTGGCAATTGGGGCTGTTTGTAAGACTCCCGAATCGCGTTTGGCTTTGGTGTTGTTGGGTGAAATGAGAGAAATGGGGTGGGTTCCTTCCGGGCGTACGTATACTAGTGTGATTGGGGCGTGTGTGAAGCAGGGGAATATGGTGGAGGCGCTAAGGCTCAAGGATGAAATGGTGAGTTGTGGGGAATCGATTGATTTGGTTGTTGCTACAAGCTTGATGAAGGGTTATTGTGCGCAAGGGAAGTTGGATGCTGCTTTGGATTTGTTCAACATGATTATGCAGGATGGACTTATTCCTAACAGAATCACTTATGCAACTGTGATAGGATGCTGCTGTAAGAATGGGAACATGGAAAGGGCATATGAGCTTTACACCAAAATGAAACAGATGGGTATTTTGCCTGATGAGTTCATTGTGAATCATTTGGTGCGTGGATTTTTGAAATGTCGGTTACTTGAAGATGCGTCTAAGTTGTTTGACGAGGCAGTTGACTACGGTATTGCAAATGTCTTCTTGTACAATAATTTCTTAGCATGGCTTTGTAGGGAGGGTAAAGTAAGCGAAGCCCGTAGTTTATGGGATAAGATGATGCGTAATGGTGTGATACCTAATGCAGTTTCCTACAACAACATGATACATGGCTATTGCAGAATAGGGGATATGGATGGTGCAAATAATGAATTTTTGGAGATGACACAAAGGGGTTTGGAACCTACTGTTTTTACTTACTCTATTTTGATGAATGGGTATTTCAGGAACGGTAATGCCGAACGTGCTTTTAATGTCTTTGATGATATGGTGGATGCAAAGAAAATCCCCACAGGCTACACAATTAACATTGTCATTGATGGCCTGTGCAAAGTTGGCCAGACATCTGTGGCAAGTGATCTTTTGAAGAAATTTCTTGAGAAGGGTTTTATTCCTGGATGTATGACTTATAATAGTATTATACATGGGTTTACCAGGGAGGGTGCCATGAATTCGGCACTGAATGTTTATAGAGAAATGTGTGAAGGTGGAGTCACTCCAACTGTTATCACTTACACCAGCCTTATTAATGGGTTATGCAAAAGCAATAAGATAGATCTTGCTTTAGAAATGTGGCATGAAATGAAAAGTAAGGGCATCAAAATGGATGTTACAGCATACTCTGCTATCATTGATGGGTTTTGCAAAAGGCGAGACATGAAAAGTGCCCGCGAACTTTTCTCGGAACTTCTGGAAGTTGGTTTATCTCCAAATACAGGTGTTTATACTAGCATGATTTGTGGCTTCCGGAATGTAAATGACATGGAAGCAGCTATTGAGTTACATAAGAAAATGATAACTGAAGGCATTCCATGTGATGTGAAAACGTACACTGCATTAATCAATGGACTGCTAAGAAAGGGTGATTTACAATCTGCATCAGATCTTTACTCAAAGATGCTTTCGAAGGGAATTACACCTGATATCAACACATATACAGTTCTAATAAATGGCCTTTGTTGCAAACGACAATTGGAGAAGGCACGCAAAACCTTGGAATACATGAATAGAAGGAGAATGACTGCTAGGGTTCATATTTATACTGCTTTGATTGTTGGGCACTTCAAGGAGGGGAATTTGCAAGAATCGTTTAGGTTGCACGATGAAATGCTAGACAAAGGCCTCGTACCTGATGACATTACTTGTGATATTCTTATAAATGGAAAGTTCAAAGGCATAAGTGCTCTTCCGAGGACTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 Exo_endo_phos_2 RNase_H RVT_1 zf-CCHC_4

Protein Analysis

824

Amino Acids

91.86

Weight (kDa)

8.04

Isoelectric Point (pI)

30.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 177 - 221 6.3e-06 PPR repeat family
PPR PF01535 281 - 309 6.6e-06 PPR repeat
PPR_3 PF13812 300 - 362 1.7e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 313 - 361 2.5e-11 PPR repeat family
PPR_1 PF12854 343 - 376 1.1e-08 PPR repeat
PPR_2 PF13041 347 - 385 6.9e-10 PPR repeat family
PPR PF01535 350 - 380 2.8e-07 PPR repeat
PPR_2 PF13041 417 - 456 3.4e-10 PPR repeat family
PPR_long PF17177 427 - 531 9.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 440 - 497 1.1e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 447 - 473 5.7e-07 PPR repeat
PPR_2 PF13041 451 - 499 6.6e-17 PPR repeat family
PPR_3 PF13812 478 - 530 3.9e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 482 - 515 6e-11 PPR repeat
PPR PF01535 489 - 516 2.6e-06 PPR repeat
PPR_2 PF13041 493 - 535 1.6e-10 PPR repeat family
TPR_24 PF23276 536 - 653 1.2e-10 Fungal tetratrico peptide repeats
PPR_3 PF13812 545 - 601 2.4e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 552 - 584 4.6e-06 PPR repeat
PPR_2 PF13041 559 - 605 1.8e-16 PPR repeat family
PPR_long PF17177 568 - 713 4.7e-12 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 588 - 620 6.4e-12 PPR repeat
PPR_2 PF13041 591 - 640 1.4e-17 PPR repeat family
PPR PF01535 594 - 624 3.1e-09 PPR repeat
PPR_3 PF13812 616 - 670 1.1e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 622 - 653 9.3e-07 PPR repeat
PPR_2 PF13041 627 - 673 2.9e-10 PPR repeat family
PPR_2 PF13041 661 - 709 1.8e-11 PPR repeat family
PPR_3 PF13812 686 - 741 6.7e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 697 - 744 7.4e-17 PPR repeat family
PPR PF01535 700 - 729 5.2e-06 PPR repeat
PPR_1 PF12854 727 - 759 7.1e-08 PPR repeat
PPR_2 PF13041 768 - 812 3.4e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000187)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20170 FvH4_4g06831
rosa_chinensis RchiOBHm_Chr1g0321991 RchiOBHm_Chr2g0161811
rosa_laevigata RLG00000015375 RLG00000018377
rosa_multiflora Rmu_co8011754.1_g000001 Rmu_co8019986.1_g000001 Rmu_co8067766.1_g000001 Rmu_co8156128.1_g000001 Rmu_co8219154.1_g000003 Rmu_co8297235.1_g000001 Rmu_co8345223.1_g000001 Rmu_co8364775.1_g000001 Rmu_co8465075.1_g000001 Rmu_sc0000007.1_g000002 Rmu_sc0000054.1_g000015 Rmu_sc0000054.1_g000023 Rmu_sc0000145.1_g000010 Rmu_sc0000166.1_g000006 Rmu_sc0000218.1_g000012 Rmu_sc0000339.1_g000008 Rmu_sc0000342.1_g000015 Rmu_sc0000464.1_g000008 Rmu_sc0000620.1_g000003 Rmu_sc0000638.1_g000017 Rmu_sc0000890.1_g000007 Rmu_sc0000894.1_g000024 Rmu_sc0000909.1_g000010 Rmu_sc0000928.1_g000020 Rmu_sc0000977.1_g000007 Rmu_sc0001022.1_g000003 Rmu_sc0001035.1_g000037 Rmu_sc0001060.1_g000008 Rmu_sc0001168.1_g000005 Rmu_sc0001173.1_g000022 Rmu_sc0001179.1_g000016 Rmu_sc0001408.1_g000003 Rmu_sc0001455.1_g000005 Rmu_sc0001484.1_g000008 Rmu_sc0001585.1_g000010 Rmu_sc0001702.1_g000030 Rmu_sc0001755.1_g000063 Rmu_sc0001903.1_g000009 Rmu_sc0002099.1_g000001 Rmu_sc0002142.1_g000012 Rmu_sc0002158.1_g000014 Rmu_sc0002307.1_g000009 Rmu_sc0002358.1_g000013 Rmu_sc0002421.1_g000002 Rmu_sc0002426.1_g000003 Rmu_sc0002438.1_g000001 Rmu_sc0002461.1_g000001 Rmu_sc0002508.1_g000003 Rmu_sc0002532.1_g000056 Rmu_sc0002583.1_g000002 Rmu_sc0002927.1_g000033 Rmu_sc0003033.1_g000040 Rmu_sc0003271.1_g000006 Rmu_sc0003381.1_g000006 Rmu_sc0003507.1_g000007 Rmu_sc0003578.1_g000003 Rmu_sc0003693.1_g000004 Rmu_sc0003693.1_g000005 Rmu_sc0003704.1_g000003 Rmu_sc0003840.1_g000002 Rmu_sc0004084.1_g000026 Rmu_sc0004193.1_g000007 Rmu_sc0004234.1_g000033 Rmu_sc0004235.1_g000025 Rmu_sc0004435.1_g000006 Rmu_sc0004727.1_g000003 Rmu_sc0004741.1_g000001 Rmu_sc0004926.1_g000004 Rmu_sc0005365.1_g000001 Rmu_sc0005392.1_g000007 Rmu_sc0005415.1_g000001 Rmu_sc0005539.1_g000007 Rmu_sc0005708.1_g000017 Rmu_sc0006317.1_g000007 Rmu_sc0006453.1_g000054 Rmu_sc0006536.1_g000004 Rmu_sc0006740.1_g000022 Rmu_sc0006763.1_g000012 Rmu_sc0007115.1_g000002 Rmu_sc0007115.1_g000010 Rmu_sc0007129.1_g000017 Rmu_sc0007131.1_g000010 Rmu_sc0008373.1_g000008 Rmu_sc0009005.1_g000008 Rmu_sc0009252.1_g000007 Rmu_sc0009599.1_g000022 Rmu_sc0009785.1_g000027 Rmu_sc0009891.1_g000003 Rmu_sc0010099.1_g000010 Rmu_sc0010266.1_g000008 Rmu_sc0010334.1_g000003 Rmu_sc0010381.1_g000001 Rmu_sc0010496.1_g000003 Rmu_sc0010496.1_g000004 Rmu_sc0010546.1_g000003 Rmu_sc0011482.1_g000006 Rmu_sc0011875.1_g000010 Rmu_sc0011917.1_g000006 Rmu_sc0012293.1_g000004 Rmu_sc0012847.1_g000003 Rmu_sc0013773.1_g000001 Rmu_sc0016060.1_g000003 Rmu_sc0016170.1_g000013 Rmu_sc0016393.1_g000002 Rmu_sc0017589.1_g000001 Rmu_sc0017786.1_g000005 Rmu_sc0018326.1_g000002 Rmu_sc0020760.1_g000001 Rmu_sc0021262.1_g000003 Rmu_sc0023851.1_g000002 Rmu_sc0024044.1_g000001 Rmu_sc0026820.1_g000001 Rmu_sc0032306.1_g000001 Rmu_sc0035666.1_g000001 Rmu_sc0036881.1_g000001 Rmu_sc0037030.1_g000001 Rmu_sc0037158.1_g000001 Rmu_ssc0000016.1_g000002 Rmu_ssc0000044.1_g000012 Rmu_ssc0000044.1_g000014 Rmu_ssc0000050.1_g000064 Rmu_ssc0000064.1_g000022 Rmu_ssc0000154.1_g000037 Rmu_ssc0000178.1_g000013 Rmu_ssc0000217.1_g000022 Rmu_ssc0000392.1_g000004
rosa_roxburghii Rroxscaffold_3G00242000 Rroxscaffold_3G00242530 Rroxscaffold_6G00406010
rosa_rugosa Rorug01G0062000 Rorug02G0224000 Rorug02G0262900 Rorug02G0263000 Rorug02G0358900 Rorug02G0360500 Rorug02G0378500.1 Rorug02G0378600 Rorug02G0394700 Rorug03G0122900 Rorug03G0254600 Rorug03G0254700 Rorug03G0254800 Rorug04G0042200.1 Rorug04G0378100 Rorug05G0053400 Rorug05G0238600 Rorug05G0364500 Rorug05G0562000 Rorug06G0089300 Rorug06G0089400 Rorug06G0145700 Rorug07G0111600 Rorug07G0229500.1 Rorug07G0287600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1683, 2429
Acc16I TGCGCA 2 cut(s) 385, 978
AccB1I GGYRCC 1 cut(s) 1715
AccBSI CCGCTC 1 cut(s) 185
AccI GTMKAC 2 cut(s) 237, 845
AccII CGCG 5 cut(s) 204, 288, 294, 781, 1944
AccIII TCCGGA 1 cut(s) 2019
AciI CCGC 8 cut(s) 95, 98, 183, 288, 313, 618, 732, 1942
AclWI GGATC 1 cut(s) 167
AcoI YGGCCR 1 cut(s) 1609
AcsI RAATTY 4 cut(s) 1424, 1643, 1723, 2347
AcuI CTGAAG 1 cut(s) 2094
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 5 cut(s) 692, 841, 1262, 2102, 2400
AfiI CCNNNNNNNGG 1 cut(s) 1988
AflIII ACRYGT 1 cut(s) 637
AhdI GACNNNNNGTC 1 cut(s) 288
AhlI ACTAGT 1 cut(s) 848
AjnI CCWGG 3 cut(s) 217, 1666, 1706
AluBI AGCT 7 cut(s) 212, 441, 499, 725, 957, 1111, 2045
AluI AGCT 7 cut(s) 212, 441, 499, 725, 957, 1111, 2045
Alw21I GWGCWC 1 cut(s) 2458
Alw26I GTCTC 2 cut(s) 275, 1920
AlwI GGATC 1 cut(s) 167
Aor13HI TCCGGA 1 cut(s) 2019
AoxI GGCC 6 cut(s) 334, 681, 1594, 1609, 2224, 2392
ApeKI GCWGC 4 cut(s) 722, 995, 1074, 2042
ApoI RAATTY 4 cut(s) 1424, 1643, 1723, 2347
AseI ATTAAT 2 cut(s) 1800, 2111
Asp700I GAANNNNTTC 1 cut(s) 2079
AspLEI GCGC 6 cut(s) 206, 296, 386, 629, 895, 979
AspS9I GGNCC 1 cut(s) 335
AsuC2I CCSGG 1 cut(s) 835
AsuHPI GGTGA 6 cut(s) 18, 206, 632, 815, 928, 2147
AsuII TTCGAA 1 cut(s) 2177
BaeGI GKGCMC 2 cut(s) 1942, 2334
BalI TGGCCA 1 cut(s) 1611
BanI GGYRCC 1 cut(s) 1715
BauI CACGAG 1 cut(s) 579
BbsI GAAGAC 1 cut(s) 1246
Bbv12I GWGCWC 1 cut(s) 2458
BbvI GCAGC 4 cut(s) 734, 982, 1061, 2054
BccI CCATC 5 cut(s) 1022, 1126, 1403, 1586, 1901
BceAI ACGGC 3 cut(s) 233, 247, 561
BciT130I CCWGG 3 cut(s) 219, 1668, 1708
BcnI CCSGG 1 cut(s) 835
BcoDI GTCTC 2 cut(s) 275, 1920
BcuI ACTAGT 1 cut(s) 848
BfaI CTAG 4 cut(s) 849, 2001, 2298, 2384
BfoI RGCGCY 1 cut(s) 896
BglII AGATCT 2 cut(s) 1828, 2155
BisI GCNGC 6 cut(s) 98, 723, 732, 996, 1075, 2043
BlsI GCNGC 6 cut(s) 99, 724, 733, 997, 1076, 2044
BmcAI AGTACT 1 cut(s) 692
Bme1390I CCNGG 4 cut(s) 219, 835, 1668, 1708
BmeRI GACNNNNNGTC 1 cut(s) 288
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 3 cut(s) 828, 1455, 1717
BmrFI CCNGG 4 cut(s) 219, 835, 1668, 1708
BmrI ACTGGG 1 cut(s) 171
BmuI ACTGGG 1 cut(s) 171
BpiI GAAGAC 1 cut(s) 1246
Bpu10I CCTNAGC 1 cut(s) 896
Bpu14I TTCGAA 1 cut(s) 2177
BpuEI CTTGAG 3 cut(s) 521, 887, 1670
BpuMI CCSGG 1 cut(s) 835
Bsa29I ATCGAT 1 cut(s) 932
BsaAI YACGTR 1 cut(s) 843
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 5 cut(s) 217, 684, 1707, 2265, 2462
BsaWI WCCGGW 1 cut(s) 2019
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 1 cut(s) 1988
Bse1I ACTGG 1 cut(s) 177
Bse3DI GCAATG 1 cut(s) 302
BseAI TCCGGA 1 cut(s) 2019
BseBI CCWGG 3 cut(s) 219, 1668, 1708
BseCI ATCGAT 1 cut(s) 932
BseDI CCNNGG 5 cut(s) 217, 684, 1707, 2265, 2462
BseGI GGATG 9 cut(s) 472, 913, 997, 1033, 1076, 1414, 1552, 1676, 1885
BseLI CCNNNNNNNGG 1 cut(s) 1988
BseMI GCAATG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 177
BseRI GAGGAG 9 cut(s) 13, 67, 185, 330, 333, 336, 339, 692, 701
BseSI GKGCMC 2 cut(s) 1942, 2334
BseXI GCAGC 4 cut(s) 734, 982, 1061, 2054
Bsh1236I CGCG 5 cut(s) 204, 288, 294, 781, 1944
Bsh1285I CGRYCG 2 cut(s) 292, 370
BshFI GGCC 6 cut(s) 336, 683, 1596, 1611, 2226, 2394
BshNI GGYRCC 1 cut(s) 1715
BshVI ATCGAT 1 cut(s) 932
BsiEI CGRYCG 2 cut(s) 292, 370
BsiHKAI GWGCWC 1 cut(s) 2458
BsiSI CCGG 6 cut(s) 324, 333, 353, 674, 834, 2020
BsiWI CGTACG 1 cut(s) 839
BslI CCNNNNNNNGG 1 cut(s) 1988
BsmAI GTCTC 2 cut(s) 275, 1920
BsmI GAATGC 1 cut(s) 2079
BsnI GGCC 6 cut(s) 336, 683, 1596, 1611, 2226, 2394
Bsp119I TTCGAA 1 cut(s) 2177
Bsp1286I GDGCHC 3 cut(s) 1942, 2334, 2458
Bsp13I TCCGGA 1 cut(s) 2019
Bsp1407I TGTACA 1 cut(s) 1260
Bsp143I GATC 5 cut(s) 172, 367, 1630, 1828, 2155
BspACI CCGC 8 cut(s) 95, 98, 183, 288, 313, 618, 732, 1942
BspANI GGCC 6 cut(s) 336, 683, 1596, 1611, 2226, 2394
BspDI ATCGAT 1 cut(s) 932
BspEI TCCGGA 1 cut(s) 2019
BspFNI CGCG 5 cut(s) 204, 288, 294, 781, 1944
BspHI TCATGA 2 cut(s) 642, 2471
BspLI GGNNCC 3 cut(s) 828, 1455, 1717
BspPI GGATC 1 cut(s) 167
BspQI GCTCTTC 1 cut(s) 2463
BspT104I TTCGAA 1 cut(s) 2177
BspT107I GGYRCC 1 cut(s) 1715
BsrBI CCGCTC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 302
BsrGI TGTACA 1 cut(s) 1260
BsrI ACTGG 1 cut(s) 177
BssECI CCNNGG 5 cut(s) 217, 684, 1707, 2265, 2462
BssMI GATC 5 cut(s) 172, 367, 1630, 1828, 2155
BssNAI GTATAC 1 cut(s) 846
BssNI GRCGYC 1 cut(s) 234
BssSI CACGAG 1 cut(s) 579
BssT1I CCWWGG 1 cut(s) 2265
Bst1107I GTATAC 1 cut(s) 846
Bst2BI CACGAG 1 cut(s) 579
Bst2UI CCWGG 3 cut(s) 219, 1668, 1708
Bst4CI ACNGT 7 cut(s) 244, 1063, 1241, 1462, 1505, 1777, 2212
Bst6I CTCTTC 2 cut(s) 159, 2463
BstACI GRCGYC 1 cut(s) 234
BstAPI GCANNNNNTGC 2 cut(s) 728, 1895
BstAUI TGTACA 1 cut(s) 1260
BstBAI YACGTR 1 cut(s) 843
BstBI TTCGAA 1 cut(s) 2177
BstC8I GCNNGC 3 cut(s) 651, 1942, 2257
BstDEI CTNAG 4 cut(s) 896, 1212, 1273, 2126
BstF5I GGATG 9 cut(s) 472, 913, 997, 1033, 1076, 1414, 1552, 1676, 1885
BstFNI CGCG 5 cut(s) 204, 288, 294, 781, 1944
BstH2I RGCGCY 1 cut(s) 896
BstHHI GCGC 6 cut(s) 206, 296, 386, 629, 895, 979
BstKTI GATC 5 cut(s) 175, 370, 1633, 1831, 2158
BstMAI GTCTC 2 cut(s) 275, 1920
BstMBI GATC 5 cut(s) 172, 367, 1630, 1828, 2155
BstMCI CGRYCG 2 cut(s) 292, 370
BstMWI GCNNNNNNNGC 9 cut(s) 333, 624, 728, 731, 755, 1517, 1608, 1895, 2232
BstNI CCWGG 3 cut(s) 219, 1668, 1708
BstNSI RCATGY 1 cut(s) 641
BstSCI CCNGG 4 cut(s) 217, 833, 1666, 1706
BstSLI GKGCMC 2 cut(s) 1942, 2334
BstSNI TACGTA 1 cut(s) 843
BstUI CGCG 5 cut(s) 204, 288, 294, 781, 1944
BstV1I GCAGC 4 cut(s) 734, 982, 1061, 2054
BstV2I GAAGAC 1 cut(s) 1246
BstX2I RGATCY 2 cut(s) 1828, 2155
BstYI RGATCY 2 cut(s) 1828, 2155
BstZ17I GTATAC 1 cut(s) 846
Bsu15I ATCGAT 1 cut(s) 932
BsuRI GGCC 6 cut(s) 336, 683, 1596, 1611, 2226, 2394
BsuTUI ATCGAT 1 cut(s) 932
BtsCI GGATG 9 cut(s) 472, 913, 997, 1033, 1076, 1414, 1552, 1676, 1885
BtsI GCAGTG 1 cut(s) 2103
BtsIMutI CAGTG 2 cut(s) 1730, 2103
Cac8I GCNNGC 3 cut(s) 651, 1942, 2257
CciI TCATGA 2 cut(s) 642, 2471
CfoI GCGC 6 cut(s) 206, 296, 386, 629, 895, 979
Cfr13I GGNCC 1 cut(s) 335
ClaI ATCGAT 1 cut(s) 932
CseI GACGC 2 cut(s) 223, 1197
Csp6I GTAC 5 cut(s) 691, 840, 1261, 2101, 2399
CviQI GTAC 5 cut(s) 691, 840, 1261, 2101, 2399
DdeI CTNAG 4 cut(s) 896, 1212, 1273, 2126
DpnI GATC 5 cut(s) 174, 369, 1632, 1830, 2157
DpnII GATC 5 cut(s) 172, 367, 1630, 1828, 2155
DriI GACNNNNNGTC 1 cut(s) 288
EaeI YGGCCR 1 cut(s) 1609
Eam1104I CTCTTC 2 cut(s) 159, 2463
Eam1105I GACNNNNNGTC 1 cut(s) 288
EarI CTCTTC 2 cut(s) 159, 2463
Eco105I TACGTA 1 cut(s) 843
Eco130I CCWWGG 1 cut(s) 2265
Eco147I AGGCCT 1 cut(s) 2394
Eco32I GATATC 1 cut(s) 2197
Eco57I CTGAAG 1 cut(s) 2094
EcoRI GAATTC 1 cut(s) 1723
EcoRII CCWGG 3 cut(s) 217, 1666, 1706
EcoRV GATATC 1 cut(s) 2197
EcoT14I CCWWGG 1 cut(s) 2265
ErhI CCWWGG 1 cut(s) 2265
FauI CCCGC 1 cut(s) 1949
FauNDI CATATG 1 cut(s) 1105
FblI GTMKAC 2 cut(s) 237, 845
Fnu4HI GCNGC 6 cut(s) 98, 723, 732, 996, 1075, 2043
FokI GGATG 9 cut(s) 479, 920, 1004, 1040, 1083, 1421, 1559, 1683, 1892
Fsp4HI GCNGC 6 cut(s) 98, 723, 732, 996, 1075, 2043
FspBI CTAG 4 cut(s) 849, 2001, 2298, 2384
FspI TGCGCA 2 cut(s) 385, 978
GlaI GCGC 6 cut(s) 205, 295, 385, 628, 894, 978
GluI GCNGC 6 cut(s) 98, 723, 732, 996, 1075, 2043
HaeII RGCGCY 1 cut(s) 896
HaeIII GGCC 6 cut(s) 336, 683, 1596, 1611, 2226, 2394
HapII CCGG 6 cut(s) 324, 333, 353, 674, 834, 2020
HgaI GACGC 2 cut(s) 223, 1197
HhaI GCGC 6 cut(s) 206, 296, 386, 629, 895, 979
Hin1I GRCGYC 1 cut(s) 234
Hin6I GCGC 6 cut(s) 204, 294, 384, 627, 893, 977
HinP1I GCGC 6 cut(s) 204, 294, 384, 627, 893, 977
HincII GTYRAC 5 cut(s) 229, 238, 543, 604, 1234
HindII GTYRAC 5 cut(s) 229, 238, 543, 604, 1234
HindIII AAGCTT 1 cut(s) 955
HpaII CCGG 6 cut(s) 324, 333, 353, 674, 834, 2020
HphI GGTGA 6 cut(s) 18, 206, 632, 815, 928, 2147
Hpy166II GTNNAC 8 cut(s) 229, 238, 543, 604, 846, 1234, 1704, 2103
Hpy188I TCNGA 6 cut(s) 124, 558, 699, 1957, 2155, 2463
Hpy8I GTNNAC 8 cut(s) 229, 238, 543, 604, 846, 1234, 1704, 2103
Hpy99I CGWCG 3 cut(s) 48, 239, 248
HpyCH4III ACNGT 7 cut(s) 244, 1063, 1241, 1462, 1505, 1777, 2212
HpyCH4IV ACGT 4 cut(s) 318, 842, 1516, 2099
HpyF10VI GCNNNNNNNGC 9 cut(s) 333, 624, 728, 731, 755, 1517, 1608, 1895, 2232
HpyF3I CTNAG 4 cut(s) 896, 1212, 1273, 2126
HpySE526I ACGT 4 cut(s) 318, 842, 1516, 2099
Hsp92I GRCGYC 1 cut(s) 234
HspAI GCGC 6 cut(s) 204, 294, 384, 627, 893, 977
Kpn2I TCCGGA 1 cut(s) 2019
Kzo9I GATC 5 cut(s) 172, 367, 1630, 1828, 2155
LguI GCTCTTC 1 cut(s) 2463
LmnI GCTCC 1 cut(s) 496
Lsp1109I GCAGC 4 cut(s) 734, 982, 1061, 2054
MaeI CTAG 4 cut(s) 849, 2001, 2298, 2384
MaeII ACGT 4 cut(s) 318, 842, 1516, 2099
MaeIII GTNAC 5 cut(s) 425, 1194, 1765, 1882, 2052
MalI GATC 5 cut(s) 174, 369, 1632, 1830, 2157
MbiI CCGCTC 1 cut(s) 185
MboI GATC 5 cut(s) 172, 367, 1630, 1828, 2155
MboII GAAGA 6 cut(s) 16, 146, 1214, 1246, 1651, 2450
MfeI CAATTG 2 cut(s) 750, 2243
MflI RGATCY 2 cut(s) 1828, 2155
MhlI GDGCHC 3 cut(s) 1942, 2334, 2458
MlsI TGGCCA 1 cut(s) 1611
MluNI TGGCCA 1 cut(s) 1611
MlyI GAGTC 4 cut(s) 277, 323, 763, 1773
MmeI TCCRAC 2 cut(s) 969, 1796
Mox20I TGGCCA 1 cut(s) 1611
MroI TCCGGA 1 cut(s) 2019
MroXI GAANNNNTTC 1 cut(s) 2079
MscI TGGCCA 1 cut(s) 1611
MseI TTAA 8 cut(s) 59, 266, 309, 483, 1524, 1578, 1800, 2111
MslI CAYNNNNRTG 4 cut(s) 266, 1019, 1121, 1874
Msp20I TGGCCA 1 cut(s) 1611
MspA1I CMGCKG 1 cut(s) 725
MspI CCGG 6 cut(s) 324, 333, 353, 674, 834, 2020
MspR9I CCNGG 4 cut(s) 219, 835, 1668, 1708
MunI CAATTG 2 cut(s) 750, 2243
Mva1269I GAATGC 1 cut(s) 2079
MvaI CCWGG 3 cut(s) 219, 1668, 1708
MvnI CGCG 5 cut(s) 204, 288, 294, 781, 1944
MwoI GCNNNNNNNGC 9 cut(s) 333, 624, 728, 731, 755, 1517, 1608, 1895, 2232
NciI CCSGG 1 cut(s) 835
NdeI CATATG 1 cut(s) 1105
NdeII GATC 5 cut(s) 172, 367, 1630, 1828, 2155
NlaIV GGNNCC 3 cut(s) 828, 1455, 1717
NmeAIII GCCGAG 1 cut(s) 709
NmuCI GTSAC 1 cut(s) 1765
NsbI TGCGCA 2 cut(s) 385, 978
NspI RCATGY 1 cut(s) 641
NspV TTCGAA 1 cut(s) 2177
PagI TCATGA 2 cut(s) 642, 2471
PceI AGGCCT 1 cut(s) 2394
PciI ACATGT 1 cut(s) 637
PciSI GCTCTTC 1 cut(s) 2463
PcsI WCGNNNNNNNCGW 2 cut(s) 324, 1509
PctI GAATGC 1 cut(s) 2079
PdmI GAANNNNTTC 1 cut(s) 2079
PfeI GAWTC 7 cut(s) 125, 410, 776, 929, 1047, 1162, 2357
Pfl23II CGTACG 1 cut(s) 839
PflFI GACNNNGTC 1 cut(s) 242
PfoI TCCNGGA 1 cut(s) 1666
PkrI GCNGC 6 cut(s) 99, 724, 733, 997, 1076, 2044
Ple19I CGATCG 1 cut(s) 370
PleI GAGTC 4 cut(s) 277, 323, 763, 1772
PpsI GAGTC 4 cut(s) 277, 323, 763, 1772
Ppu21I YACGTR 1 cut(s) 843
PscI ACATGT 1 cut(s) 637
PshBI ATTAAT 2 cut(s) 1800, 2111
PsiI TTATAA 2 cut(s) 1683, 2429
Psp6I CCWGG 3 cut(s) 217, 1666, 1706
PspGI CCWGG 3 cut(s) 217, 1666, 1706
PspLI CGTACG 1 cut(s) 839
PspN4I GGNNCC 3 cut(s) 828, 1455, 1717
PspPI GGNCC 1 cut(s) 335
PsuI RGATCY 2 cut(s) 1828, 2155
PsyI GACNNNGTC 1 cut(s) 242
PvuI CGATCG 1 cut(s) 370
PvuII CAGCTG 1 cut(s) 725
RsaI GTAC 5 cut(s) 692, 841, 1262, 2102, 2400
RsaNI GTAC 5 cut(s) 691, 840, 1261, 2101, 2399
RseI CAYNNNNRTG 4 cut(s) 266, 1019, 1121, 1874
SalI GTCGAC 1 cut(s) 236
SapI GCTCTTC 1 cut(s) 2463
SaqAI TTAA 8 cut(s) 59, 266, 309, 483, 1524, 1578, 1800, 2111
SatI GCNGC 6 cut(s) 98, 723, 732, 996, 1075, 2043
Sau3AI GATC 5 cut(s) 172, 367, 1630, 1828, 2155
Sau96I GGNCC 1 cut(s) 335
ScaI AGTACT 1 cut(s) 692
SchI GAGTC 4 cut(s) 277, 323, 763, 1773
ScrFI CCNGG 4 cut(s) 219, 835, 1668, 1708
SduI GDGCHC 3 cut(s) 1942, 2334, 2458
SfuI TTCGAA 1 cut(s) 2177
SmiMI CAYNNNNRTG 4 cut(s) 266, 1019, 1121, 1874
SmlI CTYRAG 3 cut(s) 500, 902, 1649
SmoI CTYRAG 3 cut(s) 500, 902, 1649
SnaBI TACGTA 1 cut(s) 843
SpeI ACTAGT 1 cut(s) 848
SseBI AGGCCT 1 cut(s) 2394
SsiI CCGC 8 cut(s) 95, 98, 183, 288, 313, 618, 732, 1942
SspMI CTAG 4 cut(s) 849, 2001, 2298, 2384
StuI AGGCCT 1 cut(s) 2394
StyD4I CCNGG 4 cut(s) 217, 833, 1666, 1706
StyI CCWWGG 1 cut(s) 2265
TaaI ACNGT 7 cut(s) 244, 1063, 1241, 1462, 1505, 1777, 2212
TaiI ACGT 4 cut(s) 321, 845, 1519, 2102
TaqI TCGA 5 cut(s) 237, 366, 457, 932, 2177
TaqII GACCGA 1 cut(s) 185
TatI WGTACW 2 cut(s) 690, 1260
TauI GCSGC 2 cut(s) 100, 734
TfiI GAWTC 7 cut(s) 125, 410, 776, 929, 1047, 1162, 2357
Tru1I TTAA 8 cut(s) 59, 266, 309, 483, 1524, 1578, 1800, 2111
Tru9I TTAA 8 cut(s) 59, 266, 309, 483, 1524, 1578, 1800, 2111
TscAI CASTG 2 cut(s) 1737, 2110
TseFI GTSAC 1 cut(s) 1765
TseI GCWGC 4 cut(s) 722, 995, 1074, 2042
Tsp45I GTSAC 1 cut(s) 1765
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 2 cut(s) 1737, 2110
Tth111I GACNNNGTC 1 cut(s) 242
VspI ATTAAT 2 cut(s) 1800, 2111
XapI RAATTY 4 cut(s) 1424, 1643, 1723, 2347
XceI RCATGY 1 cut(s) 641
XmiI GTMKAC 2 cut(s) 237, 845
XmnI GAANNNNTTC 1 cut(s) 2079
XspI CTAG 4 cut(s) 849, 2001, 2298, 2384
ZrmI AGTACT 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.