Rorug03G0254700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000187
Physical Location & Seq
Reverse (-)
24163226 .. 24166828
3603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0254700.1

Sequence Viewer

Length: 1743 bp
ATGGGTATGTTGGGGCTTGTTATCCGAGTAAACTGTCTATTCTTGTTATCGTTCTTGGTGTGTTCTACCCCTCCGGAGGACCCCATCAAGTGTTCATCGTCAAATAACACAAACTGCACCATCACAAACTCCTACGGCATCTTCCCCGATCGAACTGTCTGTAAAGCGACTAGCGTATCATACCCTAGTACGGAGGAAGAACTCGTTAAGGTCGTGGCTGATGCAACCAAGAACAAGAAGAGCATGAAGGTGGCGACTCGTTACTCCCACAGCATTCCCAAGCTGGTTTGCCCGCGCGGCGAAGACGGGCTGATCATAAGCACCAACAACCTCAACCGTGTGGTGAAGGTCGACGTTGAAGGGATGAAGATGACGGTGGAGAGCGGCGTGACGCTTAGGCAGGTGATCAGTGAGGCTGCGAAGGTTGACTTGGCGTTGCCTTACGCACCATATTGGTGGGGGCTAACTGTCGGTGGGTTGCTAGGGACTGGTGCACATGGAAGCACATTATGGGGTAAGGGAAGCTCTGTTCATGATTATGTGGTGGGACTTAGGATTGTGAGTCCAGGAGGGCCTGAAGTTGGTTATGTCAAGGTGAGAAGTCTCGGTCAAGGTGATAAAGATCTTAATGCAGCTAAGGTTTCACTTGGAGTCTTAGGAGTAATATCACAGGTTACTCTAAACCTTCAACCCATGTTCAAGAGGTCCATCACATATTTGAGCAAGAGCGATTCTGACTTGGGAGATCAAGTGCTTAAGTTTGGCAAGCAGCATGAGTTTGCGGACATAATATGGTATCCTAGCCAGCACAAGGCAGTTTATCGAGTAGATGATCGTATCTCCTCAAACACATCCGGAAATGGTTTATATGATTTCCTTCCTTTTCGAGCAACGCCTTCAGTTGCACTGGCAGTTATAAGAACGGCAGAAGAGAACCAAGAATCAAAAAGCGATGCTGATGGGAAATGCTCTGGGGCAAAGTTGGTCACATCTGGGCTTTTATCCGATGCTTATGGTTTGACAAACAATGGTATAATCTTTACTGGATATCCTGTGATTGGATATCAGAACCGGCTTCAAGCATCTGGAACTTGCCTTGATAGTCATCAAGATTCAAGAATCACGGCATGCGCATGGGACCCAAGGATCAAGGGCGAGTTTTTTCATCAAACAACCTTCAGCATCAGCTTGTCCGTGGCCAAAAGCTTCATTCAAGACGTGCAGAAGCTAGTTGAGATTGAACCCAAATCCCTCTGTGGAGTAGAAATCTACAATGGAATCCTAATGCGCTATGTCACCGCATCAACTGCTTACCTTGGCAAACAAGTAGATGCAATTGACTTCGACTTCACTTATTACCGAAGCAAGGACCCCATGACGCCTAGGCTCCATGAAGGCATACTTGAAGAAATAGAGCAATTGGCACTGTTTAAGTACGGAGCATTGCCACATTGGGGAAAGAATAGGAACATAGCCTTTGATGGGGTGATGAAGAAGTACATACATGGTAAGGAGTTCTTGAGGGTTAAGGAAGTGTATGACCCAATGGGGCTATTCTCAAGTGAATGGAGTGACCAGGTCCTTGGAATTAAGGGTGAGGTTAACTTAGTCAAGGAAGGGTGCGCCCTAGAAGGGTTGTGCATTTGCTCACAAGACATTCATTGTGCCCCAAGCGAGGGCTACTTCTGTAGACCGGGGAAAGTATTTAGGGATGCAAGGATCTGTACTCCGATCCATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

580

Amino Acids

63.67

Weight (kDa)

7.8

Isoelectric Point (pI)

28.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 59 - 191 1.4e-18 FAD binding domain
ALO PF04030 388 - 526 1.2e-08 D-arabinono-1,4-lactone oxidase
GULLO2-like_3rd PF22906 538 - 576 1.6e-23 L-gulonolactone oxidase 2-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000187)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20170 FvH4_4g06831
rosa_chinensis RchiOBHm_Chr1g0321991 RchiOBHm_Chr2g0161811
rosa_laevigata RLG00000015375 RLG00000018377
rosa_multiflora Rmu_co8011754.1_g000001 Rmu_co8019986.1_g000001 Rmu_co8067766.1_g000001 Rmu_co8156128.1_g000001 Rmu_co8219154.1_g000003 Rmu_co8297235.1_g000001 Rmu_co8345223.1_g000001 Rmu_co8364775.1_g000001 Rmu_co8465075.1_g000001 Rmu_sc0000007.1_g000002 Rmu_sc0000054.1_g000015 Rmu_sc0000054.1_g000023 Rmu_sc0000145.1_g000010 Rmu_sc0000166.1_g000006 Rmu_sc0000218.1_g000012 Rmu_sc0000339.1_g000008 Rmu_sc0000342.1_g000015 Rmu_sc0000464.1_g000008 Rmu_sc0000620.1_g000003 Rmu_sc0000638.1_g000017 Rmu_sc0000890.1_g000007 Rmu_sc0000894.1_g000024 Rmu_sc0000909.1_g000010 Rmu_sc0000928.1_g000020 Rmu_sc0000977.1_g000007 Rmu_sc0001022.1_g000003 Rmu_sc0001035.1_g000037 Rmu_sc0001060.1_g000008 Rmu_sc0001168.1_g000005 Rmu_sc0001173.1_g000022 Rmu_sc0001179.1_g000016 Rmu_sc0001408.1_g000003 Rmu_sc0001455.1_g000005 Rmu_sc0001484.1_g000008 Rmu_sc0001585.1_g000010 Rmu_sc0001702.1_g000030 Rmu_sc0001755.1_g000063 Rmu_sc0001903.1_g000009 Rmu_sc0002099.1_g000001 Rmu_sc0002142.1_g000012 Rmu_sc0002158.1_g000014 Rmu_sc0002307.1_g000009 Rmu_sc0002358.1_g000013 Rmu_sc0002421.1_g000002 Rmu_sc0002426.1_g000003 Rmu_sc0002438.1_g000001 Rmu_sc0002461.1_g000001 Rmu_sc0002508.1_g000003 Rmu_sc0002532.1_g000056 Rmu_sc0002583.1_g000002 Rmu_sc0002927.1_g000033 Rmu_sc0003033.1_g000040 Rmu_sc0003271.1_g000006 Rmu_sc0003381.1_g000006 Rmu_sc0003507.1_g000007 Rmu_sc0003578.1_g000003 Rmu_sc0003693.1_g000004 Rmu_sc0003693.1_g000005 Rmu_sc0003704.1_g000003 Rmu_sc0003840.1_g000002 Rmu_sc0004084.1_g000026 Rmu_sc0004193.1_g000007 Rmu_sc0004234.1_g000033 Rmu_sc0004235.1_g000025 Rmu_sc0004435.1_g000006 Rmu_sc0004727.1_g000003 Rmu_sc0004741.1_g000001 Rmu_sc0004926.1_g000004 Rmu_sc0005365.1_g000001 Rmu_sc0005392.1_g000007 Rmu_sc0005415.1_g000001 Rmu_sc0005539.1_g000007 Rmu_sc0005708.1_g000017 Rmu_sc0006317.1_g000007 Rmu_sc0006453.1_g000054 Rmu_sc0006536.1_g000004 Rmu_sc0006740.1_g000022 Rmu_sc0006763.1_g000012 Rmu_sc0007115.1_g000002 Rmu_sc0007115.1_g000010 Rmu_sc0007129.1_g000017 Rmu_sc0007131.1_g000010 Rmu_sc0008373.1_g000008 Rmu_sc0009005.1_g000008 Rmu_sc0009252.1_g000007 Rmu_sc0009599.1_g000022 Rmu_sc0009785.1_g000027 Rmu_sc0009891.1_g000003 Rmu_sc0010099.1_g000010 Rmu_sc0010266.1_g000008 Rmu_sc0010334.1_g000003 Rmu_sc0010381.1_g000001 Rmu_sc0010496.1_g000003 Rmu_sc0010496.1_g000004 Rmu_sc0010546.1_g000003 Rmu_sc0011482.1_g000006 Rmu_sc0011875.1_g000010 Rmu_sc0011917.1_g000006 Rmu_sc0012293.1_g000004 Rmu_sc0012847.1_g000003 Rmu_sc0013773.1_g000001 Rmu_sc0016060.1_g000003 Rmu_sc0016170.1_g000013 Rmu_sc0016393.1_g000002 Rmu_sc0017589.1_g000001 Rmu_sc0017786.1_g000005 Rmu_sc0018326.1_g000002 Rmu_sc0020760.1_g000001 Rmu_sc0021262.1_g000003 Rmu_sc0023851.1_g000002 Rmu_sc0024044.1_g000001 Rmu_sc0026820.1_g000001 Rmu_sc0032306.1_g000001 Rmu_sc0035666.1_g000001 Rmu_sc0036881.1_g000001 Rmu_sc0037030.1_g000001 Rmu_sc0037158.1_g000001 Rmu_ssc0000016.1_g000002 Rmu_ssc0000044.1_g000012 Rmu_ssc0000044.1_g000014 Rmu_ssc0000050.1_g000064 Rmu_ssc0000064.1_g000022 Rmu_ssc0000154.1_g000037 Rmu_ssc0000178.1_g000013 Rmu_ssc0000217.1_g000022 Rmu_ssc0000392.1_g000004
rosa_roxburghii Rroxscaffold_3G00242000 Rroxscaffold_3G00242530 Rroxscaffold_6G00406010
rosa_rugosa Rorug01G0062000 Rorug02G0224000 Rorug02G0262900 Rorug02G0263000 Rorug02G0358900 Rorug02G0360500 Rorug02G0378500.1 Rorug02G0378600 Rorug02G0394700 Rorug03G0122900 Rorug03G0254600 Rorug03G0254700 Rorug03G0254800 Rorug04G0042200.1 Rorug04G0378100 Rorug05G0053400 Rorug05G0238600 Rorug05G0364500 Rorug05G0562000 Rorug06G0089300 Rorug06G0089400 Rorug06G0145700 Rorug07G0111600 Rorug07G0229500.1 Rorug07G0287600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 917
AarI CACCTGC 1 cut(s) 391
Acc16I TGCGCA 1 cut(s) 1132
Acc36I ACCTGC 1 cut(s) 391
AccBSI CCGCTC 1 cut(s) 384
AccI GTMKAC 2 cut(s) 351, 1690
AccII CGCG 2 cut(s) 295, 297
AccIII TCCGGA 2 cut(s) 73, 854
AciI CCGC 5 cut(s) 293, 297, 384, 782, 1299
AclWI GGATC 3 cut(s) 1154, 1726, 1727
AcoI YGGCCR 1 cut(s) 1197
AcuI CTGAAG 3 cut(s) 597, 882, 1162
AcyI GRCGYC 1 cut(s) 1379
AfaI GTAC 4 cut(s) 190, 1436, 1499, 1726
AflII CTTAAG 1 cut(s) 755
AgsI TTSAA 8 cut(s) 359, 689, 700, 1079, 1116, 1214, 1241, 1406
AjiI CACGTC 1 cut(s) 1219
AjnI CCWGG 2 cut(s) 565, 1575
AjuI GAANNNNNNNTTGG 2 cut(s) 413, 445
AloI GAACNNNNNNTCC 2 cut(s) 513, 545
AluBI AGCT 6 cut(s) 283, 525, 635, 1188, 1206, 1228
AluI AGCT 6 cut(s) 283, 525, 635, 1188, 1206, 1228
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 1 cut(s) 608
Alw44I GTGCAC 1 cut(s) 492
AlwI GGATC 3 cut(s) 1154, 1726, 1727
Aor13HI TCCGGA 2 cut(s) 73, 854
AoxI GGCC 2 cut(s) 572, 1197
ApaLI GTGCAC 1 cut(s) 492
ApeKI GCWGC 3 cut(s) 416, 632, 769
AspA2I CCTAGG 1 cut(s) 1382
AspLEI GCGC 4 cut(s) 297, 1133, 1290, 1625
AspS9I GGNCC 6 cut(s) 79, 572, 705, 1138, 1369, 1579
AsuC2I CCSGG 1 cut(s) 1695
AsuHPI GGTGA 7 cut(s) 355, 415, 607, 626, 1288, 1498, 1607
AvaII GGWCC 5 cut(s) 79, 705, 1138, 1369, 1579
AvrII CCTAGG 1 cut(s) 1382
BaeGI GKGCMC 2 cut(s) 496, 1669
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BalI TGGCCA 1 cut(s) 1199
BbsI GAAGAC 1 cut(s) 309
Bbv12I GWGCWC 1 cut(s) 496
BbvI GCAGC 3 cut(s) 403, 644, 781
BccI CCATC 5 cut(s) 92, 128, 716, 953, 1475
BceAI ACGGC 3 cut(s) 151, 939, 1140
BciT130I CCWGG 2 cut(s) 567, 1577
BciVI GTATCC 1 cut(s) 807
BclI TGATCA 2 cut(s) 312, 405
BcnI CCSGG 1 cut(s) 1695
BcoDI GTCTC 1 cut(s) 608
BfaI CTAG 7 cut(s) 171, 186, 482, 801, 1229, 1383, 1628
BfmI CTRYAG 1 cut(s) 1687
BfrI CTTAAG 1 cut(s) 755
BfuAI ACCTGC 1 cut(s) 391
BfuI GTATCC 1 cut(s) 807
BglI GCCNNNNNGGC 1 cut(s) 297
BglII AGATCT 1 cut(s) 622
BisI GCNGC 5 cut(s) 298, 385, 417, 633, 770
BlnI CCTAGG 1 cut(s) 1382
BlsI GCNGC 5 cut(s) 299, 386, 418, 634, 771
Bme1390I CCNGG 3 cut(s) 567, 1577, 1695
Bme18I GGWCC 5 cut(s) 79, 705, 1138, 1369, 1579
BmgBI CACGTC 1 cut(s) 1219
BmgT120I GGNCC 6 cut(s) 79, 572, 705, 1138, 1369, 1579
BmiI GGNNCC 5 cut(s) 81, 1139, 1140, 1371, 1388
BmrFI CCNGG 3 cut(s) 567, 1577, 1695
BmsI GCATC 9 cut(s) 147, 211, 943, 997, 1091, 1191, 1310, 1321, 1702
BpiI GAAGAC 1 cut(s) 309
Bpu10I CCTNAGC 2 cut(s) 395, 636
BpuEI CTTGAG 2 cut(s) 1540, 1543
BpuMI CCSGG 1 cut(s) 1695
BsaBI GATNNNNATC 2 cut(s) 621, 1104
BsaHI GRCGYC 1 cut(s) 1379
BsaJI CCNNGG 6 cut(s) 1142, 1194, 1315, 1382, 1582, 1694
BsaWI WCCGGW 2 cut(s) 73, 854
BsaXI ACNNNNNCTCC 2 cut(s) 735, 765
Bse118I RCCGGY 1 cut(s) 1071
Bse1I ACTGG 3 cut(s) 493, 912, 1048
Bse3DI GCAATG 1 cut(s) 1442
Bse8I GATNNNNATC 2 cut(s) 621, 1104
BseAI TCCGGA 2 cut(s) 73, 854
BseBI CCWGG 2 cut(s) 567, 1577
BseDI CCNNGG 6 cut(s) 1142, 1194, 1315, 1382, 1582, 1694
BseGI GGATG 3 cut(s) 369, 851, 1717
BseJI GATNNNNATC 2 cut(s) 621, 1104
BseMI GCAATG 1 cut(s) 1442
BseNI ACTGG 3 cut(s) 493, 912, 1048
BseRI GAGGAG 1 cut(s) 832
BseSI GKGCMC 2 cut(s) 496, 1669
BseXI GCAGC 3 cut(s) 403, 644, 781
BsgI GTGCAG 2 cut(s) 100, 1241
Bsh1236I CGCG 2 cut(s) 295, 297
Bsh1285I CGRYCG 1 cut(s) 151
BshFI GGCC 2 cut(s) 574, 1199
BsiEI CGRYCG 1 cut(s) 151
BsiHKAI GWGCWC 1 cut(s) 496
BsiSI CCGG 4 cut(s) 74, 855, 1072, 1694
BslFI GGGAC 3 cut(s) 499, 561, 1151
BsmAI GTCTC 1 cut(s) 608
BsmFI GGGAC 3 cut(s) 499, 561, 1151
BsmI GAATGC 1 cut(s) 273
BsnI GGCC 2 cut(s) 574, 1199
Bsp1286I GDGCHC 2 cut(s) 496, 1669
Bsp13I TCCGGA 2 cut(s) 73, 854
Bsp143I GATC 9 cut(s) 148, 312, 405, 622, 745, 832, 1146, 1719, 1731
BspACI CCGC 5 cut(s) 293, 297, 384, 782, 1299
BspANI GGCC 2 cut(s) 574, 1199
BspEI TCCGGA 2 cut(s) 73, 854
BspFNI CGCG 2 cut(s) 295, 297
BspHI TCATGA 1 cut(s) 532
BspLI GGNNCC 5 cut(s) 81, 1139, 1140, 1371, 1388
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 3 cut(s) 1154, 1726, 1727
BspQI GCTCTTC 1 cut(s) 233
BspTI CTTAAG 1 cut(s) 755
BsrBI CCGCTC 1 cut(s) 384
BsrDI GCAATG 1 cut(s) 1442
BsrFI RCCGGY 1 cut(s) 1071
BsrI ACTGG 3 cut(s) 493, 912, 1048
BssAI RCCGGY 1 cut(s) 1071
BssECI CCNNGG 6 cut(s) 1142, 1194, 1315, 1382, 1582, 1694
BssMI GATC 9 cut(s) 148, 312, 405, 622, 745, 832, 1146, 1719, 1731
BssNI GRCGYC 1 cut(s) 1379
BssT1I CCWWGG 4 cut(s) 1142, 1315, 1382, 1582
Bst2UI CCWGG 2 cut(s) 567, 1577
Bst4CI ACNGT 6 cut(s) 35, 157, 338, 376, 469, 1428
Bst6I CTCTTC 2 cut(s) 233, 924
BstACI GRCGYC 1 cut(s) 1379
BstAFI CTTAAG 1 cut(s) 755
BstAPI GCANNNNNTGC 1 cut(s) 1307
BstC8I GCNNGC 4 cut(s) 293, 767, 806, 1129
BstDEI CTNAG 5 cut(s) 395, 551, 636, 655, 1606
BstDSI CCRYGG 1 cut(s) 1194
BstF5I GGATG 3 cut(s) 369, 851, 1717
BstFNI CGCG 2 cut(s) 295, 297
BstHHI GCGC 4 cut(s) 297, 1133, 1290, 1625
BstKTI GATC 9 cut(s) 151, 315, 408, 625, 748, 835, 1149, 1722, 1734
BstMAI GTCTC 1 cut(s) 608
BstMBI GATC 9 cut(s) 148, 312, 405, 622, 745, 832, 1146, 1719, 1731
BstMCI CGRYCG 1 cut(s) 151
BstMWI GCNNNNNNNGC 2 cut(s) 297, 1307
BstNI CCWGG 2 cut(s) 567, 1577
BstNSI RCATGY 1 cut(s) 1131
BstSCI CCNGG 3 cut(s) 565, 1575, 1693
BstSFI CTRYAG 1 cut(s) 1687
BstSLI GKGCMC 2 cut(s) 496, 1669
BstUI CGCG 2 cut(s) 295, 297
BstV1I GCAGC 3 cut(s) 403, 644, 781
BstV2I GAAGAC 1 cut(s) 309
BstX2I RGATCY 2 cut(s) 622, 1719
BstXI CCANNNNNNTGG 2 cut(s) 456, 1583
BstYI RGATCY 2 cut(s) 622, 1719
BsuI GTATCC 1 cut(s) 807
BsuRI GGCC 2 cut(s) 574, 1199
BtgI CCRYGG 1 cut(s) 1194
BtgZI GCGATG 1 cut(s) 966
BtrI CACGTC 1 cut(s) 1219
BtsCI GGATG 3 cut(s) 369, 851, 1717
BtsIMutI CAGTG 3 cut(s) 415, 905, 1424
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 4 cut(s) 293, 767, 806, 1129
CciI TCATGA 1 cut(s) 532
CfoI GCGC 4 cut(s) 297, 1133, 1290, 1625
Cfr10I RCCGGY 1 cut(s) 1071
Cfr13I GGNCC 6 cut(s) 79, 572, 705, 1138, 1369, 1579
CseI GACGC 2 cut(s) 400, 1387
CsiI ACCWGGT 1 cut(s) 1575
Csp6I GTAC 4 cut(s) 189, 1435, 1498, 1725
CviQI GTAC 4 cut(s) 189, 1435, 1498, 1725
DdeI CTNAG 5 cut(s) 395, 551, 636, 655, 1606
DpnI GATC 9 cut(s) 150, 314, 407, 624, 747, 834, 1148, 1721, 1733
DpnII GATC 9 cut(s) 148, 312, 405, 622, 745, 832, 1146, 1719, 1731
EaeI YGGCCR 1 cut(s) 1197
Eam1104I CTCTTC 2 cut(s) 233, 924
EarI CTCTTC 2 cut(s) 233, 924
Eco130I CCWWGG 4 cut(s) 1142, 1315, 1382, 1582
Eco32I GATATC 2 cut(s) 1049, 1064
Eco47I GGWCC 5 cut(s) 79, 705, 1138, 1369, 1579
Eco57I CTGAAG 3 cut(s) 597, 882, 1162
EcoO109I RGGNCCY 5 cut(s) 79, 572, 1138, 1369, 1579
EcoRII CCWGG 2 cut(s) 565, 1575
EcoRV GATATC 2 cut(s) 1049, 1064
EcoT14I CCWWGG 4 cut(s) 1142, 1315, 1382, 1582
ErhI CCWWGG 4 cut(s) 1142, 1315, 1382, 1582
FalI AAGNNNNNCTT 6 cut(s) 413, 445, 1386, 1418, 1502, 1534
FaqI GGGAC 3 cut(s) 499, 561, 1151
FauI CCCGC 1 cut(s) 300
FbaI TGATCA 2 cut(s) 312, 405
FblI GTMKAC 2 cut(s) 351, 1690
Fnu4HI GCNGC 5 cut(s) 298, 385, 417, 633, 770
FokI GGATG 3 cut(s) 376, 838, 1724
Fsp4HI GCNGC 5 cut(s) 298, 385, 417, 633, 770
FspAI RTGCGCAY 1 cut(s) 1132
FspBI CTAG 7 cut(s) 171, 186, 482, 801, 1229, 1383, 1628
FspI TGCGCA 1 cut(s) 1132
GlaI GCGC 4 cut(s) 296, 1132, 1289, 1624
GluI GCNGC 5 cut(s) 298, 385, 417, 633, 770
HaeIII GGCC 2 cut(s) 574, 1199
HapII CCGG 4 cut(s) 74, 855, 1072, 1694
HgaI GACGC 2 cut(s) 400, 1387
HhaI GCGC 4 cut(s) 297, 1133, 1290, 1625
Hin1I GRCGYC 1 cut(s) 1379
Hin6I GCGC 4 cut(s) 295, 1131, 1288, 1623
HinP1I GCGC 4 cut(s) 295, 1131, 1288, 1623
HincII GTYRAC 3 cut(s) 352, 427, 1603
HindII GTYRAC 3 cut(s) 352, 427, 1603
HindIII AAGCTT 1 cut(s) 1204
HinfI GANTC 8 cut(s) 256, 562, 651, 731, 941, 1112, 1119, 1278
HpaI GTTAAC 1 cut(s) 1603
HpaII CCGG 4 cut(s) 74, 855, 1072, 1694
HphI GGTGA 7 cut(s) 355, 415, 607, 626, 1288, 1498, 1607
Hpy166II GTNNAC 6 cut(s) 31, 352, 427, 494, 1603, 1691
Hpy188I TCNGA 5 cut(s) 26, 736, 1006, 1068, 1731
Hpy188III TCNNGA 9 cut(s) 74, 533, 700, 855, 1086, 1109, 1116, 1214, 1519
Hpy8I GTNNAC 6 cut(s) 31, 352, 427, 494, 1603, 1691
Hpy99I CGWCG 1 cut(s) 356
HpyCH4III ACNGT 6 cut(s) 35, 157, 338, 376, 469, 1428
HpyCH4IV ACGT 2 cut(s) 354, 1218
HpyCH4V TGCA 9 cut(s) 117, 224, 494, 632, 905, 1222, 1334, 1641, 1715
HpyF10VI GCNNNNNNNGC 2 cut(s) 297, 1307
HpyF3I CTNAG 5 cut(s) 395, 551, 636, 655, 1606
HpySE526I ACGT 2 cut(s) 354, 1218
Hsp92I GRCGYC 1 cut(s) 1379
HspAI GCGC 4 cut(s) 295, 1131, 1288, 1623
KflI GGGWCCC 1 cut(s) 1138
Kpn2I TCCGGA 2 cut(s) 73, 854
Ksp22I TGATCA 2 cut(s) 312, 405
KspAI GTTAAC 1 cut(s) 1603
Kzo9I GATC 9 cut(s) 148, 312, 405, 622, 745, 832, 1146, 1719, 1731
LguI GCTCTTC 1 cut(s) 233
LmnI GCTCC 2 cut(s) 1392, 1439
Lsp1109I GCAGC 3 cut(s) 403, 644, 781
LweI GCATC 9 cut(s) 147, 211, 943, 997, 1091, 1191, 1310, 1321, 1702
MabI ACCWGGT 1 cut(s) 1575
MaeI CTAG 7 cut(s) 171, 186, 482, 801, 1229, 1383, 1628
MaeII ACGT 2 cut(s) 354, 1218
MaeIII GTNAC 6 cut(s) 260, 388, 673, 985, 1294, 1571
MalI GATC 9 cut(s) 150, 314, 407, 624, 747, 834, 1148, 1721, 1733
MbiI CCGCTC 1 cut(s) 384
MboI GATC 9 cut(s) 148, 312, 405, 622, 745, 832, 1146, 1719, 1731
MboII GAAGA 8 cut(s) 133, 209, 250, 314, 379, 941, 1418, 1504
MfeI CAATTG 2 cut(s) 1335, 1418
MflI RGATCY 2 cut(s) 622, 1719
MhlI GDGCHC 2 cut(s) 496, 1669
MlsI TGGCCA 1 cut(s) 1199
MluCI AATT 4 cut(s) 1335, 1418, 1587, 1738
MluNI TGGCCA 1 cut(s) 1199
MlyI GAGTC 3 cut(s) 250, 571, 660
Mox20I TGGCCA 1 cut(s) 1199
MroI TCCGGA 2 cut(s) 73, 854
MscI TGGCCA 1 cut(s) 1199
MseI TTAA 7 cut(s) 207, 627, 756, 1431, 1527, 1590, 1602
MslI CAYNNNNRTG 4 cut(s) 248, 454, 537, 1132
Msp20I TGGCCA 1 cut(s) 1199
MspCI CTTAAG 1 cut(s) 755
MspI CCGG 4 cut(s) 74, 855, 1072, 1694
MspR9I CCNGG 3 cut(s) 567, 1577, 1695
MunI CAATTG 2 cut(s) 1335, 1418
Mva1269I GAATGC 1 cut(s) 273
MvaI CCWGG 2 cut(s) 567, 1577
MvnI CGCG 2 cut(s) 295, 297
MwoI GCNNNNNNNGC 2 cut(s) 297, 1307
NciI CCSGG 1 cut(s) 1695
NdeII GATC 9 cut(s) 148, 312, 405, 622, 745, 832, 1146, 1719, 1731
NlaIV GGNNCC 5 cut(s) 81, 1139, 1140, 1371, 1388
NmuCI GTSAC 4 cut(s) 388, 985, 1294, 1571
NsbI TGCGCA 1 cut(s) 1132
NspI RCATGY 1 cut(s) 1131
PaeI GCATGC 1 cut(s) 1131
PagI TCATGA 1 cut(s) 532
PaqCI CACCTGC 1 cut(s) 391
PciSI GCTCTTC 1 cut(s) 233
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 1 cut(s) 273
PfeI GAWTC 5 cut(s) 731, 941, 1112, 1119, 1278
PflFI GACNNNGTC 1 cut(s) 1577
PfoI TCCNGGA 1 cut(s) 565
PkrI GCNGC 5 cut(s) 299, 386, 418, 634, 771
Ple19I CGATCG 1 cut(s) 151
PleI GAGTC 3 cut(s) 250, 570, 659
PpsI GAGTC 3 cut(s) 250, 570, 659
PpuMI RGGWCCY 4 cut(s) 79, 1138, 1369, 1579
PsiI TTATAA 1 cut(s) 917
Psp5II RGGWCCY 4 cut(s) 79, 1138, 1369, 1579
Psp6I CCWGG 2 cut(s) 565, 1575
PspGI CCWGG 2 cut(s) 565, 1575
PspN4I GGNNCC 5 cut(s) 81, 1139, 1140, 1371, 1388
PspPI GGNCC 6 cut(s) 79, 572, 705, 1138, 1369, 1579
PspPPI RGGWCCY 4 cut(s) 79, 1138, 1369, 1579
PsuI RGATCY 2 cut(s) 622, 1719
PsyI GACNNNGTC 1 cut(s) 1577
PvuI CGATCG 1 cut(s) 151
RsaI GTAC 4 cut(s) 190, 1436, 1499, 1726
RsaNI GTAC 4 cut(s) 189, 1435, 1498, 1725
RseI CAYNNNNRTG 4 cut(s) 248, 454, 537, 1132
SalI GTCGAC 1 cut(s) 350
SapI GCTCTTC 1 cut(s) 233
SaqAI TTAA 7 cut(s) 207, 627, 756, 1431, 1527, 1590, 1602
SatI GCNGC 5 cut(s) 298, 385, 417, 633, 770
Sau3AI GATC 9 cut(s) 148, 312, 405, 622, 745, 832, 1146, 1719, 1731
Sau96I GGNCC 6 cut(s) 79, 572, 705, 1138, 1369, 1579
SchI GAGTC 3 cut(s) 250, 571, 660
ScrFI CCNGG 3 cut(s) 567, 1577, 1695
SduI GDGCHC 2 cut(s) 496, 1669
SexAI ACCWGGT 1 cut(s) 1575
SfaNI GCATC 9 cut(s) 147, 211, 943, 997, 1091, 1191, 1310, 1321, 1702
SfcI CTRYAG 1 cut(s) 1687
SinI GGWCC 5 cut(s) 79, 705, 1138, 1369, 1579
SmiMI CAYNNNNRTG 4 cut(s) 248, 454, 537, 1132
SmlI CTYRAG 3 cut(s) 755, 1519, 1558
SmoI CTYRAG 3 cut(s) 755, 1519, 1558
SphI GCATGC 1 cut(s) 1131
Sse9I AATT 4 cut(s) 1335, 1418, 1587, 1738
SsiI CCGC 5 cut(s) 293, 297, 384, 782, 1299
SspMI CTAG 7 cut(s) 171, 186, 482, 801, 1229, 1383, 1628
StyD4I CCNGG 3 cut(s) 565, 1575, 1693
StyI CCWWGG 4 cut(s) 1142, 1315, 1382, 1582
TaaI ACNGT 6 cut(s) 35, 157, 338, 376, 469, 1428
TaiI ACGT 2 cut(s) 357, 1221
TaqI TCGA 5 cut(s) 151, 351, 823, 886, 1344
TaqII GACCGA 1 cut(s) 596
TasI AATT 4 cut(s) 1335, 1418, 1587, 1738
TatI WGTACW 2 cut(s) 1497, 1724
TauI GCSGC 2 cut(s) 300, 387
TfiI GAWTC 5 cut(s) 731, 941, 1112, 1119, 1278
Tru1I TTAA 7 cut(s) 207, 627, 756, 1431, 1527, 1590, 1602
Tru9I TTAA 7 cut(s) 207, 627, 756, 1431, 1527, 1590, 1602
TscAI CASTG 3 cut(s) 415, 912, 1431
TseFI GTSAC 4 cut(s) 388, 985, 1294, 1571
TseI GCWGC 3 cut(s) 416, 632, 769
Tsp45I GTSAC 4 cut(s) 388, 985, 1294, 1571
TspDTI ATGAA 9 cut(s) 84, 260, 380, 521, 1154, 1198, 1407, 1505, 1649
TspGWI ACGGA 3 cut(s) 206, 1183, 1452
TspRI CASTG 3 cut(s) 415, 912, 1431
Tth111I GACNNNGTC 1 cut(s) 1577
Vha464I CTTAAG 1 cut(s) 755
VneI GTGCAC 1 cut(s) 492
VpaK11BI GGWCC 5 cut(s) 79, 705, 1138, 1369, 1579
XceI RCATGY 1 cut(s) 1131
XmaJI CCTAGG 1 cut(s) 1382
XmiI GTMKAC 2 cut(s) 351, 1690
XspI CTAG 7 cut(s) 171, 186, 482, 801, 1229, 1383, 1628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.