ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 373 |
| AatII | GACGTC | 1 cut(s) | 377 |
| AciI | CCGC | 2 cut(s) | 224, 404 |
| AclWI | GGATC | 2 cut(s) | 392, 394 |
| AcuI | CTGAAG | 1 cut(s) | 75 |
| AcyI | GRCGYC | 1 cut(s) | 374 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 88, 89, 170 |
| AflIII | ACRYGT | 1 cut(s) | 114 |
| AgsI | TTSAA | 1 cut(s) | 247 |
| AjnI | CCWGG | 2 cut(s) | 188, 393 |
| AluBI | AGCT | 1 cut(s) | 6 |
| AluI | AGCT | 1 cut(s) | 6 |
| Alw21I | GWGCWC | 2 cut(s) | 8, 243 |
| AlwI | GGATC | 2 cut(s) | 392, 394 |
| AoxI | GGCC | 1 cut(s) | 91 |
| ApeKI | GCWGC | 1 cut(s) | 23 |
| AspLEI | GCGC | 2 cut(s) | 81, 292 |
| AspS9I | GGNCC | 2 cut(s) | 91, 256 |
| AsuC2I | CCSGG | 1 cut(s) | 484 |
| AsuHPI | GGTGA | 1 cut(s) | 379 |
| AvaII | GGWCC | 1 cut(s) | 256 |
| AxyI | CCTNAGG | 1 cut(s) | 451 |
| BanII | GRGCYC | 1 cut(s) | 8 |
| Bbv12I | GWGCWC | 2 cut(s) | 8, 243 |
| BbvI | GCAGC | 1 cut(s) | 10 |
| BccI | CCATC | 1 cut(s) | 329 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 461 |
| BciT130I | CCWGG | 2 cut(s) | 190, 395 |
| BcnI | CCSGG | 1 cut(s) | 484 |
| BfaI | CTAG | 2 cut(s) | 27, 314 |
| BfmI | CTRYAG | 1 cut(s) | 261 |
| BisI | GCNGC | 1 cut(s) | 24 |
| BlsI | GCNGC | 1 cut(s) | 25 |
| Bme1390I | CCNGG | 3 cut(s) | 190, 395, 484 |
| Bme18I | GGWCC | 1 cut(s) | 256 |
| BmgT120I | GGNCC | 2 cut(s) | 91, 256 |
| BmiI | GGNNCC | 2 cut(s) | 258, 360 |
| BmrFI | CCNGG | 3 cut(s) | 190, 395, 484 |
| BmsI | GCATC | 2 cut(s) | 4, 348 |
| BpmI | CTGGAG | 2 cut(s) | 211, 377 |
| BpuMI | CCSGG | 1 cut(s) | 484 |
| BsaHI | GRCGYC | 1 cut(s) | 374 |
| BsaJI | CCNNGG | 3 cut(s) | 18, 94, 293 |
| BsaWI | WCCGGW | 1 cut(s) | 414 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 88, 89, 170 |
| Bse118I | RCCGGY | 1 cut(s) | 346 |
| Bse1I | ACTGG | 1 cut(s) | 386 |
| Bse21I | CCTNAGG | 1 cut(s) | 451 |
| Bse3DI | GCAATG | 1 cut(s) | 206 |
| BseBI | CCWGG | 2 cut(s) | 190, 395 |
| BseDI | CCNNGG | 3 cut(s) | 18, 94, 293 |
| BseGI | GGATG | 1 cut(s) | 180 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 88, 89, 170 |
| BseMI | GCAATG | 1 cut(s) | 206 |
| BseMII | CTCAG | 1 cut(s) | 101 |
| BseNI | ACTGG | 1 cut(s) | 386 |
| BseRI | GAGGAG | 1 cut(s) | 242 |
| BseXI | GCAGC | 1 cut(s) | 10 |
| BshFI | GGCC | 1 cut(s) | 93 |
| BsiHKAI | GWGCWC | 2 cut(s) | 8, 243 |
| BsiSI | CCGG | 3 cut(s) | 347, 415, 483 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 88, 89, 170 |
| BsmI | GAATGC | 1 cut(s) | 484 |
| BsnI | GGCC | 1 cut(s) | 93 |
| Bsp1286I | GDGCHC | 2 cut(s) | 8, 243 |
| Bsp143I | GATC | 5 cut(s) | 156, 265, 384, 399, 468 |
| Bsp19I | CCATGG | 2 cut(s) | 18, 293 |
| BspACI | CCGC | 2 cut(s) | 224, 404 |
| BspANI | GGCC | 1 cut(s) | 93 |
| BspCNI | CTCAG | 1 cut(s) | 100 |
| BspLI | GGNNCC | 2 cut(s) | 258, 360 |
| BspMAI | CTGCAG | 1 cut(s) | 265 |
| BspPI | GGATC | 2 cut(s) | 392, 394 |
| BspQI | GCTCTTC | 1 cut(s) | 248 |
| BsrDI | GCAATG | 1 cut(s) | 206 |
| BsrFI | RCCGGY | 1 cut(s) | 346 |
| BsrI | ACTGG | 1 cut(s) | 386 |
| BssAI | RCCGGY | 1 cut(s) | 346 |
| BssECI | CCNNGG | 3 cut(s) | 18, 94, 293 |
| BssMI | GATC | 5 cut(s) | 156, 265, 384, 399, 468 |
| BssNI | GRCGYC | 1 cut(s) | 374 |
| BssT1I | CCWWGG | 3 cut(s) | 18, 94, 293 |
| Bst2UI | CCWGG | 2 cut(s) | 190, 395 |
| Bst4CI | ACNGT | 1 cut(s) | 142 |
| Bst6I | CTCTTC | 1 cut(s) | 248 |
| BstACI | GRCGYC | 1 cut(s) | 374 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 238 |
| BstC8I | GCNNGC | 1 cut(s) | 408 |
| BstDEI | CTNAG | 2 cut(s) | 87, 451 |
| BstDSI | CCRYGG | 2 cut(s) | 18, 293 |
| BstF5I | GGATG | 1 cut(s) | 180 |
| BstHHI | GCGC | 2 cut(s) | 81, 292 |
| BstKTI | GATC | 5 cut(s) | 159, 268, 387, 402, 471 |
| BstMBI | GATC | 5 cut(s) | 156, 265, 384, 399, 468 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 23, 32, 238, 289, 317 |
| BstNI | CCWGG | 2 cut(s) | 190, 395 |
| BstSCI | CCNGG | 3 cut(s) | 188, 393, 482 |
| BstSFI | CTRYAG | 1 cut(s) | 261 |
| BstV1I | GCAGC | 1 cut(s) | 10 |
| BstX2I | RGATCY | 1 cut(s) | 399 |
| BstYI | RGATCY | 1 cut(s) | 399 |
| Bsu36I | CCTNAGG | 1 cut(s) | 451 |
| BsuRI | GGCC | 1 cut(s) | 93 |
| BtgI | CCRYGG | 2 cut(s) | 18, 293 |
| BtsCI | GGATG | 1 cut(s) | 180 |
| Cac8I | GCNNGC | 1 cut(s) | 408 |
| CfoI | GCGC | 2 cut(s) | 81, 292 |
| Cfr10I | RCCGGY | 1 cut(s) | 346 |
| Cfr13I | GGNCC | 2 cut(s) | 91, 256 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 25, 60 |
| CviAII | CATG | 5 cut(s) | 19, 169, 294, 308, 343 |
| CviJI | RGCY | 7 cut(s) | 6, 23, 35, 93, 163, 300, 444 |
| CviKI_1 | RGCY | 7 cut(s) | 6, 23, 35, 93, 163, 300, 444 |
| DdeI | CTNAG | 2 cut(s) | 87, 451 |
| DpnI | GATC | 5 cut(s) | 158, 267, 386, 401, 470 |
| DpnII | GATC | 5 cut(s) | 156, 265, 384, 399, 468 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 373 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 373 |
| Eam1104I | CTCTTC | 1 cut(s) | 248 |
| EarI | CTCTTC | 1 cut(s) | 248 |
| Ecl136II | GAGCTC | 1 cut(s) | 6 |
| Eco130I | CCWWGG | 3 cut(s) | 18, 94, 293 |
| Eco24I | GRGCYC | 1 cut(s) | 8 |
| Eco47I | GGWCC | 1 cut(s) | 256 |
| Eco53kI | GAGCTC | 1 cut(s) | 6 |
| Eco57I | CTGAAG | 1 cut(s) | 75 |
| Eco81I | CCTNAGG | 1 cut(s) | 451 |
| EcoICRI | GAGCTC | 1 cut(s) | 6 |
| EcoRII | CCWGG | 2 cut(s) | 188, 393 |
| EcoT14I | CCWWGG | 3 cut(s) | 18, 94, 293 |
| EcoT38I | GRGCYC | 1 cut(s) | 8 |
| ErhI | CCWWGG | 3 cut(s) | 18, 94, 293 |
| FaeI | CATG | 5 cut(s) | 22, 172, 297, 311, 346 |
| FaiI | YATR | 9 cut(s) | 20, 170, 295, 303, 309, 333, 344, 431, 437 |
| FatI | CATG | 5 cut(s) | 18, 168, 293, 307, 342 |
| FauI | CCCGC | 1 cut(s) | 411 |
| Fnu4HI | GCNGC | 1 cut(s) | 24 |
| FokI | GGATG | 1 cut(s) | 187 |
| FriOI | GRGCYC | 1 cut(s) | 8 |
| Fsp4HI | GCNGC | 1 cut(s) | 24 |
| FspBI | CTAG | 2 cut(s) | 27, 314 |
| GlaI | GCGC | 2 cut(s) | 80, 291 |
| GluI | GCNGC | 1 cut(s) | 24 |
| GsuI | CTGGAG | 2 cut(s) | 211, 377 |
| HaeIII | GGCC | 1 cut(s) | 93 |
| HapII | CCGG | 3 cut(s) | 347, 415, 483 |
| HhaI | GCGC | 2 cut(s) | 81, 292 |
| Hin1I | GRCGYC | 1 cut(s) | 374 |
| Hin1II | CATG | 5 cut(s) | 22, 172, 297, 311, 346 |
| Hin6I | GCGC | 2 cut(s) | 79, 290 |
| HinP1I | GCGC | 2 cut(s) | 79, 290 |
| HpaII | CCGG | 3 cut(s) | 347, 415, 483 |
| HphI | GGTGA | 1 cut(s) | 379 |
| Hpy188I | TCNGA | 1 cut(s) | 256 |
| Hpy188III | TCNNGA | 2 cut(s) | 146, 464 |
| Hpy99I | CGWCG | 1 cut(s) | 376 |
| HpyAV | CCTTC | 1 cut(s) | 50 |
| HpyCH4III | ACNGT | 1 cut(s) | 142 |
| HpyCH4IV | ACGT | 2 cut(s) | 114, 374 |
| HpyCH4V | TGCA | 3 cut(s) | 211, 263, 353 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 23, 32, 238, 289, 317 |
| HpyF3I | CTNAG | 2 cut(s) | 87, 451 |
| HpySE526I | ACGT | 2 cut(s) | 114, 374 |
| Hsp92I | GRCGYC | 1 cut(s) | 374 |
| Hsp92II | CATG | 5 cut(s) | 22, 172, 297, 311, 346 |
| HspAI | GCGC | 2 cut(s) | 79, 290 |
| Kzo9I | GATC | 5 cut(s) | 156, 265, 384, 399, 468 |
| LguI | GCTCTTC | 1 cut(s) | 248 |
| LmnI | GCTCC | 2 cut(s) | 203, 229 |
| Lsp1109I | GCAGC | 1 cut(s) | 10 |
| LweI | GCATC | 2 cut(s) | 4, 348 |
| MaeI | CTAG | 2 cut(s) | 27, 314 |
| MaeII | ACGT | 2 cut(s) | 114, 374 |
| MaeIII | GTNAC | 1 cut(s) | 146 |
| MalI | GATC | 5 cut(s) | 158, 267, 386, 401, 470 |
| MboI | GATC | 5 cut(s) | 156, 265, 384, 399, 468 |
| MboII | GAAGA | 1 cut(s) | 235 |
| MflI | RGATCY | 1 cut(s) | 399 |
| MhlI | GDGCHC | 2 cut(s) | 8, 243 |
| MmeI | TCCRAC | 1 cut(s) | 234 |
| MnlI | CCTC | 7 cut(s) | 49, 57, 186, 211, 220, 228, 401 |
| MslI | CAYNNNNRTG | 1 cut(s) | 173 |
| MspI | CCGG | 3 cut(s) | 347, 415, 483 |
| MspR9I | CCNGG | 3 cut(s) | 190, 395, 484 |
| Mva1269I | GAATGC | 1 cut(s) | 484 |
| MvaI | CCWGG | 2 cut(s) | 190, 395 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 23, 32, 238, 289, 317 |
| NciI | CCSGG | 1 cut(s) | 484 |
| NcoI | CCATGG | 2 cut(s) | 18, 293 |
| NdeII | GATC | 5 cut(s) | 156, 265, 384, 399, 468 |
| NlaIII | CATG | 5 cut(s) | 22, 172, 297, 311, 346 |
| NlaIV | GGNNCC | 2 cut(s) | 258, 360 |
| NmuCI | GTSAC | 1 cut(s) | 146 |
| PciSI | GCTCTTC | 1 cut(s) | 248 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 280 |
| PctI | GAATGC | 1 cut(s) | 484 |
| PfoI | TCCNGGA | 1 cut(s) | 393 |
| PkrI | GCNGC | 1 cut(s) | 25 |
| Psp124BI | GAGCTC | 1 cut(s) | 8 |
| Psp6I | CCWGG | 2 cut(s) | 188, 393 |
| PspGI | CCWGG | 2 cut(s) | 188, 393 |
| PspN4I | GGNNCC | 2 cut(s) | 258, 360 |
| PspPI | GGNCC | 2 cut(s) | 91, 256 |
| PstI | CTGCAG | 1 cut(s) | 265 |
| PsuI | RGATCY | 1 cut(s) | 399 |
| RseI | CAYNNNNRTG | 1 cut(s) | 173 |
| SacI | GAGCTC | 1 cut(s) | 8 |
| SapI | GCTCTTC | 1 cut(s) | 248 |
| SatI | GCNGC | 1 cut(s) | 24 |
| Sau3AI | GATC | 5 cut(s) | 156, 265, 384, 399, 468 |
| Sau96I | GGNCC | 2 cut(s) | 91, 256 |
| ScrFI | CCNGG | 3 cut(s) | 190, 395, 484 |
| SduI | GDGCHC | 2 cut(s) | 8, 243 |
| SetI | ASST | 8 cut(s) | 8, 41, 61, 100, 117, 360, 377, 452 |
| SfaNI | GCATC | 2 cut(s) | 4, 348 |
| SfcI | CTRYAG | 1 cut(s) | 261 |
| SinI | GGWCC | 1 cut(s) | 256 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 173 |
| SsiI | CCGC | 2 cut(s) | 224, 404 |
| SspMI | CTAG | 2 cut(s) | 27, 314 |
| SstI | GAGCTC | 1 cut(s) | 8 |
| StyD4I | CCNGG | 3 cut(s) | 188, 393, 482 |
| StyI | CCWWGG | 3 cut(s) | 18, 94, 293 |
| TaaI | ACNGT | 1 cut(s) | 142 |
| TaiI | ACGT | 2 cut(s) | 117, 377 |
| TaqI | TCGA | 1 cut(s) | 471 |
| TseFI | GTSAC | 1 cut(s) | 146 |
| TseI | GCWGC | 1 cut(s) | 23 |
| Tsp45I | GTSAC | 1 cut(s) | 146 |
| TspDTI | ATGAA | 1 cut(s) | 157 |
| VpaK11BI | GGWCC | 1 cut(s) | 256 |
| XspI | CTAG | 2 cut(s) | 27, 314 |
| ZraI | GACGTC | 1 cut(s) | 375 |