Rorug06G0089300

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000187
Physical Location & Seq
Reverse (-)
11894328 .. 11895415
1088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0089300.1

Sequence Viewer

Length: 705 bp
ATGGCGACTAGTCACGCAGAAGTCCCCGGCGAGCCAACCCAGACCGGAACAGCCATCCTCGAAACCGCAACCGCCGCCATCCAAGGCTTCGGCCCCGTCAACAAGATCCACCAGCACCTCTGCGCCTTCCACTTCTATGGCCACGACATGACACGTCAGGTCGAAGCCCACCATTTCTGCGGGCACCAGAATGAGGAGGTGCGTCAGTGCCTCATCTACGACAGCCCTGAGGCCGACGCTAAGCTCATCGGCCTCGAGTACATCGTCTCGGAGAGACTCTTCTTGACTCTCCCTGACGACGAGAAGAAGCTGTGGCACTCTCACGAGTATGAGGTGAAGAGTGGGGTTTTGTTCATGCCCGGGGTGCCGGGACCGATCCAGCGTCAGGATCTGGACAAGGTTTGTAAGACCTATGGGAAGGTGTTCCATTTCTGGCAGGTTGATAGGGGCGACAATCTACCTCTTGGGGTGCCACAGGTGATGTTGGCATTGACTAGGGATGGTCAGCTGTATGAGGATCTTGCTCGAAATGTGGAGAAGAGGTTTGGGATATCCTTTGAGAAGGAGAGGGAGAACCGAGCTTATATGACCGGGCCGACTCTCGGAGTGCATCCTTTGGCAAACGGAGGAGGAAAAGGGTTGAAGACGGAGCTGAGGGAGGTGGACGTTCAGCCGGTCGATTCTGTTCCGAGGGTCTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

234

Amino Acids

26.29

Weight (kDa)

5.82

Isoelectric Point (pI)

30.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1264 PF06884 30 - 196 5.4e-75 Protein of unknown function (DUF1264)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000187)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20170 FvH4_4g06831
rosa_chinensis RchiOBHm_Chr1g0321991 RchiOBHm_Chr2g0161811
rosa_laevigata RLG00000015375 RLG00000018377
rosa_multiflora Rmu_co8011754.1_g000001 Rmu_co8019986.1_g000001 Rmu_co8067766.1_g000001 Rmu_co8156128.1_g000001 Rmu_co8219154.1_g000003 Rmu_co8297235.1_g000001 Rmu_co8345223.1_g000001 Rmu_co8364775.1_g000001 Rmu_co8465075.1_g000001 Rmu_sc0000007.1_g000002 Rmu_sc0000054.1_g000015 Rmu_sc0000054.1_g000023 Rmu_sc0000145.1_g000010 Rmu_sc0000166.1_g000006 Rmu_sc0000218.1_g000012 Rmu_sc0000339.1_g000008 Rmu_sc0000342.1_g000015 Rmu_sc0000464.1_g000008 Rmu_sc0000620.1_g000003 Rmu_sc0000638.1_g000017 Rmu_sc0000890.1_g000007 Rmu_sc0000894.1_g000024 Rmu_sc0000909.1_g000010 Rmu_sc0000928.1_g000020 Rmu_sc0000977.1_g000007 Rmu_sc0001022.1_g000003 Rmu_sc0001035.1_g000037 Rmu_sc0001060.1_g000008 Rmu_sc0001168.1_g000005 Rmu_sc0001173.1_g000022 Rmu_sc0001179.1_g000016 Rmu_sc0001408.1_g000003 Rmu_sc0001455.1_g000005 Rmu_sc0001484.1_g000008 Rmu_sc0001585.1_g000010 Rmu_sc0001702.1_g000030 Rmu_sc0001755.1_g000063 Rmu_sc0001903.1_g000009 Rmu_sc0002099.1_g000001 Rmu_sc0002142.1_g000012 Rmu_sc0002158.1_g000014 Rmu_sc0002307.1_g000009 Rmu_sc0002358.1_g000013 Rmu_sc0002421.1_g000002 Rmu_sc0002426.1_g000003 Rmu_sc0002438.1_g000001 Rmu_sc0002461.1_g000001 Rmu_sc0002508.1_g000003 Rmu_sc0002532.1_g000056 Rmu_sc0002583.1_g000002 Rmu_sc0002927.1_g000033 Rmu_sc0003033.1_g000040 Rmu_sc0003271.1_g000006 Rmu_sc0003381.1_g000006 Rmu_sc0003507.1_g000007 Rmu_sc0003578.1_g000003 Rmu_sc0003693.1_g000004 Rmu_sc0003693.1_g000005 Rmu_sc0003704.1_g000003 Rmu_sc0003840.1_g000002 Rmu_sc0004084.1_g000026 Rmu_sc0004193.1_g000007 Rmu_sc0004234.1_g000033 Rmu_sc0004235.1_g000025 Rmu_sc0004435.1_g000006 Rmu_sc0004727.1_g000003 Rmu_sc0004741.1_g000001 Rmu_sc0004926.1_g000004 Rmu_sc0005365.1_g000001 Rmu_sc0005392.1_g000007 Rmu_sc0005415.1_g000001 Rmu_sc0005539.1_g000007 Rmu_sc0005708.1_g000017 Rmu_sc0006317.1_g000007 Rmu_sc0006453.1_g000054 Rmu_sc0006536.1_g000004 Rmu_sc0006740.1_g000022 Rmu_sc0006763.1_g000012 Rmu_sc0007115.1_g000002 Rmu_sc0007115.1_g000010 Rmu_sc0007129.1_g000017 Rmu_sc0007131.1_g000010 Rmu_sc0008373.1_g000008 Rmu_sc0009005.1_g000008 Rmu_sc0009252.1_g000007 Rmu_sc0009599.1_g000022 Rmu_sc0009785.1_g000027 Rmu_sc0009891.1_g000003 Rmu_sc0010099.1_g000010 Rmu_sc0010266.1_g000008 Rmu_sc0010334.1_g000003 Rmu_sc0010381.1_g000001 Rmu_sc0010496.1_g000003 Rmu_sc0010496.1_g000004 Rmu_sc0010546.1_g000003 Rmu_sc0011482.1_g000006 Rmu_sc0011875.1_g000010 Rmu_sc0011917.1_g000006 Rmu_sc0012293.1_g000004 Rmu_sc0012847.1_g000003 Rmu_sc0013773.1_g000001 Rmu_sc0016060.1_g000003 Rmu_sc0016170.1_g000013 Rmu_sc0016393.1_g000002 Rmu_sc0017589.1_g000001 Rmu_sc0017786.1_g000005 Rmu_sc0018326.1_g000002 Rmu_sc0020760.1_g000001 Rmu_sc0021262.1_g000003 Rmu_sc0023851.1_g000002 Rmu_sc0024044.1_g000001 Rmu_sc0026820.1_g000001 Rmu_sc0032306.1_g000001 Rmu_sc0035666.1_g000001 Rmu_sc0036881.1_g000001 Rmu_sc0037030.1_g000001 Rmu_sc0037158.1_g000001 Rmu_ssc0000016.1_g000002 Rmu_ssc0000044.1_g000012 Rmu_ssc0000044.1_g000014 Rmu_ssc0000050.1_g000064 Rmu_ssc0000064.1_g000022 Rmu_ssc0000154.1_g000037 Rmu_ssc0000178.1_g000013 Rmu_ssc0000217.1_g000022 Rmu_ssc0000392.1_g000004
rosa_roxburghii Rroxscaffold_3G00242000 Rroxscaffold_3G00242530 Rroxscaffold_6G00406010
rosa_rugosa Rorug01G0062000 Rorug02G0224000 Rorug02G0262900 Rorug02G0263000 Rorug02G0358900 Rorug02G0360500 Rorug02G0378500.1 Rorug02G0378600 Rorug02G0394700 Rorug03G0122900 Rorug03G0254600 Rorug03G0254700 Rorug03G0254800 Rorug04G0042200.1 Rorug04G0378100 Rorug05G0053400 Rorug05G0238600 Rorug05G0364500 Rorug05G0562000 Rorug06G0089300 Rorug06G0089400 Rorug06G0145700 Rorug07G0111600 Rorug07G0229500.1 Rorug07G0287600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 427
AccB1I GGYRCC 3 cut(s) 183, 364, 469
AciI CCGC 4 cut(s) 66, 72, 75, 180
AclWI GGATC 4 cut(s) 100, 370, 396, 525
AcoI YGGCCR 1 cut(s) 139
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 3 cut(s) 193, 385, 602
AflIII ACRYGT 1 cut(s) 152
AgsI TTSAA 1 cut(s) 643
AhlI ACTAGT 1 cut(s) 8
AjiI CACGTC 1 cut(s) 155
AluBI AGCT 5 cut(s) 244, 310, 508, 581, 652
AluI AGCT 5 cut(s) 244, 310, 508, 581, 652
Alw26I GTCTC 2 cut(s) 268, 271
AlwI GGATC 4 cut(s) 100, 370, 396, 525
AlwNI CAGNNNCTG 1 cut(s) 391
Ama87I CYCGRG 2 cut(s) 254, 359
AoxI GGCC 5 cut(s) 91, 139, 231, 250, 593
Asp700I GAANNNNTTC 1 cut(s) 422
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 3 cut(s) 92, 371, 593
AsuC2I CCSGG 5 cut(s) 27, 360, 361, 369, 592
AsuHPI GGTGA 2 cut(s) 346, 490
AvaI CYCGRG 2 cut(s) 254, 359
AvaII GGWCC 1 cut(s) 371
AxyI CCTNAGG 1 cut(s) 228
BaeGI GKGCMC 1 cut(s) 186
BalI TGGCCA 1 cut(s) 141
BanI GGYRCC 3 cut(s) 183, 364, 469
BauI CACGAG 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 650
BbvCI CCTCAGC 1 cut(s) 653
BccI CCATC 3 cut(s) 62, 86, 494
BcnI CCSGG 5 cut(s) 27, 360, 361, 369, 592
BcoDI GTCTC 2 cut(s) 268, 271
BcuI ACTAGT 1 cut(s) 8
BfaI CTAG 2 cut(s) 9, 495
BfuAI ACCTGC 1 cut(s) 427
BisI GCNGC 1 cut(s) 75
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 1 cut(s) 76
Bme1390I CCNGG 5 cut(s) 27, 360, 361, 369, 592
Bme18I GGWCC 1 cut(s) 371
BmeT110I CYCGRG 2 cut(s) 254, 359
BmgBI CACGTC 1 cut(s) 155
BmgT120I GGNCC 3 cut(s) 92, 371, 593
BmiI GGNNCC 5 cut(s) 94, 185, 366, 372, 471
BmrFI CCNGG 5 cut(s) 27, 360, 361, 369, 592
BmsI GCATC 1 cut(s) 619
BpiI GAAGAC 1 cut(s) 650
Bpu10I CCTNAGC 1 cut(s) 653
Bpu1102I GCTNAGC 1 cut(s) 240
BpuMI CCSGG 5 cut(s) 27, 360, 361, 369, 592
BsaJI CCNNGG 5 cut(s) 25, 82, 359, 360, 689
BsaWI WCCGGW 1 cut(s) 44
BsaXI ACNNNNNCTCC 2 cut(s) 527, 557
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 385, 602
Bse118I RCCGGY 1 cut(s) 673
Bse21I CCTNAGG 1 cut(s) 228
BseDI CCNNGG 5 cut(s) 25, 82, 359, 360, 689
BseGI GGATG 4 cut(s) 54, 78, 505, 610
BseLI CCNNNNNNNGG 3 cut(s) 193, 385, 602
BseMII CTCAG 2 cut(s) 219, 644
BseRI GAGGAG 2 cut(s) 209, 642
BseSI GKGCMC 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 678
BshFI GGCC 5 cut(s) 93, 141, 233, 252, 595
BshNI GGYRCC 3 cut(s) 183, 364, 469
BsiEI CGRYCG 1 cut(s) 678
BsiHKCI CYCGRG 2 cut(s) 254, 359
BsiSI CCGG 6 cut(s) 27, 45, 360, 368, 591, 674
BslFI GGGAC 2 cut(s) 8, 384
BslI CCNNNNNNNGG 3 cut(s) 193, 385, 602
BsmAI GTCTC 2 cut(s) 268, 271
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 2 cut(s) 8, 384
BsnI GGCC 5 cut(s) 93, 141, 233, 252, 595
BsoBI CYCGRG 2 cut(s) 254, 359
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 4 cut(s) 105, 375, 388, 517
Bsp1720I GCTNAGC 1 cut(s) 240
BspACI CCGC 4 cut(s) 66, 72, 75, 180
BspANI GGCC 5 cut(s) 93, 141, 233, 252, 595
BspCNI CTCAG 2 cut(s) 220, 645
BspLI GGNNCC 5 cut(s) 94, 185, 366, 372, 471
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 4 cut(s) 100, 370, 396, 525
BspT107I GGYRCC 3 cut(s) 183, 364, 469
BsrFI RCCGGY 1 cut(s) 673
BssAI RCCGGY 1 cut(s) 673
BssECI CCNNGG 5 cut(s) 25, 82, 359, 360, 689
BssMI GATC 4 cut(s) 105, 375, 388, 517
BssSI CACGAG 1 cut(s) 323
BssT1I CCWWGG 1 cut(s) 82
Bst2BI CACGAG 1 cut(s) 323
Bst6I CTCTTC 3 cut(s) 284, 332, 533
BstC8I GCNNGC 2 cut(s) 32, 182
BstDEI CTNAG 3 cut(s) 228, 240, 653
BstF5I GGATG 4 cut(s) 54, 78, 505, 610
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 4 cut(s) 108, 378, 391, 520
BstMAI GTCTC 2 cut(s) 268, 271
BstMBI GATC 4 cut(s) 105, 375, 388, 517
BstMCI CGRYCG 1 cut(s) 678
BstMWI GCNNNNNNNGC 2 cut(s) 74, 364
BstSCI CCNGG 5 cut(s) 25, 358, 359, 367, 590
BstSLI GKGCMC 1 cut(s) 186
BstV2I GAAGAC 1 cut(s) 650
BstX2I RGATCY 3 cut(s) 105, 388, 517
BstXI CCANNNNNNTGG 1 cut(s) 137
BstYI RGATCY 3 cut(s) 105, 388, 517
Bsu36I CCTNAGG 1 cut(s) 228
BsuRI GGCC 5 cut(s) 93, 141, 233, 252, 595
BtrI CACGTC 1 cut(s) 155
BtsCI GGATG 4 cut(s) 54, 78, 505, 610
BtsIMutI CAGTG 1 cut(s) 212
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 2 cut(s) 32, 182
CaiI CAGNNNCTG 1 cut(s) 391
CfoI GCGC 1 cut(s) 125
Cfr10I RCCGGY 1 cut(s) 673
Cfr13I GGNCC 3 cut(s) 92, 371, 593
Cfr9I CCCGGG 1 cut(s) 359
CseI GACGC 3 cut(s) 191, 245, 371
Csp6I GTAC 1 cut(s) 259
CviAII CATG 2 cut(s) 148, 355
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 3 cut(s) 228, 240, 653
DpnI GATC 4 cut(s) 107, 377, 390, 519
DpnII GATC 4 cut(s) 105, 375, 388, 517
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 3 cut(s) 284, 332, 533
EarI CTCTTC 3 cut(s) 284, 332, 533
Eco130I CCWWGG 1 cut(s) 82
Eco32I GATATC 1 cut(s) 552
Eco47I GGWCC 1 cut(s) 371
Eco81I CCTNAGG 1 cut(s) 228
Eco88I CYCGRG 2 cut(s) 254, 359
EcoRV GATATC 1 cut(s) 552
EcoT14I CCWWGG 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 82
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 2 cut(s) 151, 358
FaiI YATR 8 cut(s) 138, 149, 330, 356, 414, 513, 585, 587
FaqI GGGAC 2 cut(s) 8, 384
FatI CATG 2 cut(s) 147, 354
FauI CCCGC 1 cut(s) 173
Fnu4HI GCNGC 1 cut(s) 75
FokI GGATG 4 cut(s) 41, 65, 512, 597
Fsp4HI GCNGC 1 cut(s) 75
FspBI CTAG 2 cut(s) 9, 495
GlaI GCGC 1 cut(s) 124
GluI GCNGC 1 cut(s) 75
HaeIII GGCC 5 cut(s) 93, 141, 233, 252, 595
HapII CCGG 6 cut(s) 27, 45, 360, 368, 591, 674
HgaI GACGC 3 cut(s) 191, 245, 371
HhaI GCGC 1 cut(s) 125
Hin1II CATG 2 cut(s) 151, 358
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 4 cut(s) 276, 286, 598, 680
HpaII CCGG 6 cut(s) 27, 45, 360, 368, 591, 674
HphI GGTGA 2 cut(s) 346, 490
Hpy166II GTNNAC 2 cut(s) 100, 664
Hpy188I TCNGA 3 cut(s) 271, 605, 690
Hpy188III TCNNGA 4 cut(s) 283, 323, 386, 392
Hpy8I GTNNAC 2 cut(s) 100, 664
Hpy99I CGWCG 2 cut(s) 239, 302
HpyAV CCTTC 3 cut(s) 136, 412, 556
HpyCH4IV ACGT 2 cut(s) 154, 666
HpyCH4V TGCA 1 cut(s) 610
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 364
HpyF3I CTNAG 3 cut(s) 228, 240, 653
HpySE526I ACGT 2 cut(s) 154, 666
Hsp92II CATG 2 cut(s) 151, 358
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 4 cut(s) 105, 375, 388, 517
LmnI GCTCC 1 cut(s) 649
LweI GCATC 1 cut(s) 619
MaeI CTAG 2 cut(s) 9, 495
MaeII ACGT 2 cut(s) 154, 666
MaeIII GTNAC 1 cut(s) 11
MalI GATC 4 cut(s) 107, 377, 390, 519
MboI GATC 4 cut(s) 105, 375, 388, 517
MboII GAAGA 5 cut(s) 271, 316, 349, 550, 655
MflI RGATCY 3 cut(s) 105, 388, 517
MhlI GDGCHC 1 cut(s) 186
MlsI TGGCCA 1 cut(s) 141
MluNI TGGCCA 1 cut(s) 141
MlyI GAGTC 3 cut(s) 270, 280, 592
Mox20I TGGCCA 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 422
MscI TGGCCA 1 cut(s) 141
MslI CAYNNNNRTG 3 cut(s) 135, 189, 327
Msp20I TGGCCA 1 cut(s) 141
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 6 cut(s) 27, 45, 360, 368, 591, 674
MspR9I CCNGG 5 cut(s) 27, 360, 361, 369, 592
MwoI GCNNNNNNNGC 2 cut(s) 74, 364
NciI CCSGG 5 cut(s) 27, 360, 361, 369, 592
NdeII GATC 4 cut(s) 105, 375, 388, 517
NlaIII CATG 2 cut(s) 151, 358
NlaIV GGNNCC 5 cut(s) 94, 185, 366, 372, 471
NmuCI GTSAC 1 cut(s) 11
PaeR7I CTCGAG 1 cut(s) 254
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PdmI GAANNNNTTC 1 cut(s) 422
PfeI GAWTC 1 cut(s) 680
PkrI GCNGC 1 cut(s) 76
PleI GAGTC 3 cut(s) 270, 280, 592
PpsI GAGTC 3 cut(s) 270, 280, 592
PspN4I GGNNCC 5 cut(s) 94, 185, 366, 372, 471
PspPI GGNCC 3 cut(s) 92, 371, 593
PspXI VCTCGAGB 1 cut(s) 254
PstNI CAGNNNCTG 1 cut(s) 391
PsuI RGATCY 3 cut(s) 105, 388, 517
PvuII CAGCTG 1 cut(s) 508
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
RseI CAYNNNNRTG 3 cut(s) 135, 189, 327
SatI GCNGC 1 cut(s) 75
Sau3AI GATC 4 cut(s) 105, 375, 388, 517
Sau96I GGNCC 3 cut(s) 92, 371, 593
SchI GAGTC 3 cut(s) 270, 280, 592
ScrFI CCNGG 5 cut(s) 27, 360, 361, 369, 592
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 1 cut(s) 619
Sfr274I CTCGAG 1 cut(s) 254
SinI GGWCC 1 cut(s) 371
SlaI CTCGAG 1 cut(s) 254
SmaI CCCGGG 1 cut(s) 361
SmiMI CAYNNNNRTG 3 cut(s) 135, 189, 327
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SpeI ACTAGT 1 cut(s) 8
SsiI CCGC 4 cut(s) 66, 72, 75, 180
SspMI CTAG 2 cut(s) 9, 495
StyD4I CCNGG 5 cut(s) 25, 358, 359, 367, 590
StyI CCWWGG 1 cut(s) 82
TaiI ACGT 2 cut(s) 157, 669
TaqI TCGA 5 cut(s) 60, 162, 255, 526, 678
TaqII GACCGA 1 cut(s) 388
TatI WGTACW 1 cut(s) 258
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 1 cut(s) 680
TscAI CASTG 1 cut(s) 212
TseFI GTSAC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 1 cut(s) 343
TspGWI ACGGA 2 cut(s) 639, 662
TspMI CCCGGG 1 cut(s) 359
TspRI CASTG 1 cut(s) 212
VpaK11BI GGWCC 1 cut(s) 371
XhoI CTCGAG 1 cut(s) 254
XmaI CCCGGG 1 cut(s) 359
XmnI GAANNNNTTC 1 cut(s) 422
XspI CTAG 2 cut(s) 9, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.