Rmu_sc0002307.1_g000009

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002307.1
OG0000187
Physical Location & Seq
Reverse (-)
62214 .. 63695
1482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002307.1_g000009.1.cds

Sequence Viewer

Length: 813 bp
atggtggggaagttgctcactgctcgagagtacaagacccgatccttccttgggatgttctgcaatgcatggcagatcaatggatccttgcgtgtggaggagacagacggccagagagttcttttcattttgtctgatccttcggatagagttcatatctggaagggtgccctgtgggggtacaatcatgccccggtcgcgctggcatgttatgatggtgtgacgcccgtggaaaaggcccaacagaatttttccactcccacagcctcatattggataacactgcaaggtattcctcatgcgtttcggtctgaaagagtgatgactatgattggctacacacttggggattttcaggagatcgataagacggggaagaaggccggaaaacttcgtattcggggcttgccttgctcggggccggcgttggaggaggatgatgacggggctgagaggacggtgttgggatcgcaaactgcacatgcaccgctactgactttgattggaggtatgcagatgcagccgggagcttcagcttcgagttcggggacgaaaggaggcagcagcctggttttgaatgctagggttttgcttcatggaaaaaccctagtttttaagacccctatccctcagctagttgtggaacctcctctgagttccttcttgaacaaacctcggcgacaagtggtgatcagaaagcttcaggaaactgctctgacaccggagagaattactggtaaacggagaggtcgatctgaggagggagtgactatctcccacaagaagctaaagcttagcttgggtgtaggctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 zf-CCHC_4

Protein Analysis

270

Amino Acids

29.48

Weight (kDa)

9.78

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000187)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20170 FvH4_4g06831
rosa_chinensis RchiOBHm_Chr1g0321991 RchiOBHm_Chr2g0161811
rosa_laevigata RLG00000015375 RLG00000018377
rosa_multiflora Rmu_co8011754.1_g000001 Rmu_co8019986.1_g000001 Rmu_co8067766.1_g000001 Rmu_co8156128.1_g000001 Rmu_co8219154.1_g000003 Rmu_co8297235.1_g000001 Rmu_co8345223.1_g000001 Rmu_co8364775.1_g000001 Rmu_co8465075.1_g000001 Rmu_sc0000007.1_g000002 Rmu_sc0000054.1_g000015 Rmu_sc0000054.1_g000023 Rmu_sc0000145.1_g000010 Rmu_sc0000166.1_g000006 Rmu_sc0000218.1_g000012 Rmu_sc0000339.1_g000008 Rmu_sc0000342.1_g000015 Rmu_sc0000464.1_g000008 Rmu_sc0000620.1_g000003 Rmu_sc0000638.1_g000017 Rmu_sc0000890.1_g000007 Rmu_sc0000894.1_g000024 Rmu_sc0000909.1_g000010 Rmu_sc0000928.1_g000020 Rmu_sc0000977.1_g000007 Rmu_sc0001022.1_g000003 Rmu_sc0001035.1_g000037 Rmu_sc0001060.1_g000008 Rmu_sc0001168.1_g000005 Rmu_sc0001173.1_g000022 Rmu_sc0001179.1_g000016 Rmu_sc0001408.1_g000003 Rmu_sc0001455.1_g000005 Rmu_sc0001484.1_g000008 Rmu_sc0001585.1_g000010 Rmu_sc0001702.1_g000030 Rmu_sc0001755.1_g000063 Rmu_sc0001903.1_g000009 Rmu_sc0002099.1_g000001 Rmu_sc0002142.1_g000012 Rmu_sc0002158.1_g000014 Rmu_sc0002307.1_g000009 Rmu_sc0002358.1_g000013 Rmu_sc0002421.1_g000002 Rmu_sc0002426.1_g000003 Rmu_sc0002438.1_g000001 Rmu_sc0002461.1_g000001 Rmu_sc0002508.1_g000003 Rmu_sc0002532.1_g000056 Rmu_sc0002583.1_g000002 Rmu_sc0002927.1_g000033 Rmu_sc0003033.1_g000040 Rmu_sc0003271.1_g000006 Rmu_sc0003381.1_g000006 Rmu_sc0003507.1_g000007 Rmu_sc0003578.1_g000003 Rmu_sc0003693.1_g000004 Rmu_sc0003693.1_g000005 Rmu_sc0003704.1_g000003 Rmu_sc0003840.1_g000002 Rmu_sc0004084.1_g000026 Rmu_sc0004193.1_g000007 Rmu_sc0004234.1_g000033 Rmu_sc0004235.1_g000025 Rmu_sc0004435.1_g000006 Rmu_sc0004727.1_g000003 Rmu_sc0004741.1_g000001 Rmu_sc0004926.1_g000004 Rmu_sc0005365.1_g000001 Rmu_sc0005392.1_g000007 Rmu_sc0005415.1_g000001 Rmu_sc0005539.1_g000007 Rmu_sc0005708.1_g000017 Rmu_sc0006317.1_g000007 Rmu_sc0006453.1_g000054 Rmu_sc0006536.1_g000004 Rmu_sc0006740.1_g000022 Rmu_sc0006763.1_g000012 Rmu_sc0007115.1_g000002 Rmu_sc0007115.1_g000010 Rmu_sc0007129.1_g000017 Rmu_sc0007131.1_g000010 Rmu_sc0008373.1_g000008 Rmu_sc0009005.1_g000008 Rmu_sc0009252.1_g000007 Rmu_sc0009599.1_g000022 Rmu_sc0009785.1_g000027 Rmu_sc0009891.1_g000003 Rmu_sc0010099.1_g000010 Rmu_sc0010266.1_g000008 Rmu_sc0010334.1_g000003 Rmu_sc0010381.1_g000001 Rmu_sc0010496.1_g000003 Rmu_sc0010496.1_g000004 Rmu_sc0010546.1_g000003 Rmu_sc0011482.1_g000006 Rmu_sc0011875.1_g000010 Rmu_sc0011917.1_g000006 Rmu_sc0012293.1_g000004 Rmu_sc0012847.1_g000003 Rmu_sc0013773.1_g000001 Rmu_sc0016060.1_g000003 Rmu_sc0016170.1_g000013 Rmu_sc0016393.1_g000002 Rmu_sc0017589.1_g000001 Rmu_sc0017786.1_g000005 Rmu_sc0018326.1_g000002 Rmu_sc0020760.1_g000001 Rmu_sc0021262.1_g000003 Rmu_sc0023851.1_g000002 Rmu_sc0024044.1_g000001 Rmu_sc0026820.1_g000001 Rmu_sc0032306.1_g000001 Rmu_sc0035666.1_g000001 Rmu_sc0036881.1_g000001 Rmu_sc0037030.1_g000001 Rmu_sc0037158.1_g000001 Rmu_ssc0000016.1_g000002 Rmu_ssc0000044.1_g000012 Rmu_ssc0000044.1_g000014 Rmu_ssc0000050.1_g000064 Rmu_ssc0000064.1_g000022 Rmu_ssc0000154.1_g000037 Rmu_ssc0000178.1_g000013 Rmu_ssc0000217.1_g000022 Rmu_ssc0000392.1_g000004
rosa_roxburghii Rroxscaffold_3G00242000 Rroxscaffold_3G00242530 Rroxscaffold_6G00406010
rosa_rugosa Rorug01G0062000 Rorug02G0224000 Rorug02G0262900 Rorug02G0263000 Rorug02G0358900 Rorug02G0360500 Rorug02G0378500.1 Rorug02G0378600 Rorug02G0394700 Rorug03G0122900 Rorug03G0254600 Rorug03G0254700 Rorug03G0254800 Rorug04G0042200.1 Rorug04G0378100 Rorug05G0053400 Rorug05G0238600 Rorug05G0364500 Rorug05G0562000 Rorug06G0089300 Rorug06G0089400 Rorug06G0145700 Rorug07G0111600 Rorug07G0229500.1 Rorug07G0287600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 167
AccII CGCG 1 cut(s) 200
AciI CCGC 1 cut(s) 488
AclWI GGATC 5 cut(s) 36, 78, 91, 131, 475
AcoI YGGCCR 1 cut(s) 109
AcsI RAATTY 1 cut(s) 247
AcuI CTGAAG 2 cut(s) 516, 686
AcyI GRCGYC 1 cut(s) 224
AfaI GTAC 2 cut(s) 32, 182
AfiI CCNNNNNNNGG 4 cut(s) 51, 177, 273, 416
AgsI TTSAA 2 cut(s) 577, 667
AjnI CCWGG 1 cut(s) 567
AluBI AGCT 7 cut(s) 530, 536, 634, 700, 787, 793, 798
AluI AGCT 7 cut(s) 530, 536, 634, 700, 787, 793, 798
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 5 cut(s) 36, 78, 91, 131, 475
Ama87I CYCGRG 2 cut(s) 24, 415
AoxI GGCC 4 cut(s) 109, 237, 381, 419
ApeKI GCWGC 3 cut(s) 520, 561, 564
ApoI RAATTY 1 cut(s) 247
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 2 cut(s) 238, 419
AsuC2I CCSGG 2 cut(s) 194, 525
AsuHPI GGTGA 1 cut(s) 700
AvaI CYCGRG 2 cut(s) 24, 415
BaeGI GKGCMC 1 cut(s) 172
BamHI GGATCC 1 cut(s) 83
BanI GGYRCC 1 cut(s) 167
BbvCI CCTCAGC 1 cut(s) 630
BbvI GCAGC 3 cut(s) 532, 573, 576
BccI CCATC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 1 cut(s) 569
BclI TGATCA 1 cut(s) 690
BcnI CCSGG 2 cut(s) 194, 525
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 4 cut(s) 582, 608, 635, 811
BisI GCNGC 3 cut(s) 521, 562, 565
BlpI GCTNAGC 1 cut(s) 794
BlsI GCNGC 3 cut(s) 522, 563, 566
Bme1390I CCNGG 3 cut(s) 194, 525, 569
BmeT110I CYCGRG 2 cut(s) 24, 415
BmgT120I GGNCC 2 cut(s) 238, 419
BmiI GGNNCC 4 cut(s) 85, 169, 420, 645
BmrFI CCNGG 3 cut(s) 194, 525, 569
BmsI GCATC 1 cut(s) 507
Bpu10I CCTNAGC 1 cut(s) 630
Bpu1102I GCTNAGC 1 cut(s) 794
BpuMI CCSGG 2 cut(s) 194, 525
Bsa29I ATCGAT 1 cut(s) 363
BsaHI GRCGYC 1 cut(s) 224
BsaJI CCNNGG 4 cut(s) 49, 192, 228, 674
BsaWI WCCGGW 1 cut(s) 721
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 177, 273, 416
Bse118I RCCGGY 1 cut(s) 421
Bse1I ACTGG 1 cut(s) 739
Bse3DI GCAATG 1 cut(s) 70
BseBI CCWGG 1 cut(s) 569
BseCI ATCGAT 1 cut(s) 363
BseDI CCNNGG 4 cut(s) 49, 192, 228, 674
BseGI GGATG 2 cut(s) 60, 442
BseLI CCNNNNNNNGG 4 cut(s) 51, 177, 273, 416
BseMI GCAATG 1 cut(s) 70
BseMII CTCAG 4 cut(s) 441, 644, 644, 747
BseNI ACTGG 1 cut(s) 739
BseRI GAGGAG 4 cut(s) 113, 446, 639, 773
BseSI GKGCMC 1 cut(s) 172
BseXI GCAGC 3 cut(s) 532, 573, 576
BsgI GTGCAG 1 cut(s) 462
Bsh1236I CGCG 1 cut(s) 200
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 4 cut(s) 111, 239, 383, 421
BshNI GGYRCC 1 cut(s) 167
BshVI ATCGAT 1 cut(s) 363
BsiEI CGRYCG 1 cut(s) 198
BsiHKCI CYCGRG 2 cut(s) 24, 415
BsiSI CCGG 5 cut(s) 194, 384, 422, 524, 722
BslFI GGGAC 1 cut(s) 562
BslI CCNNNNNNNGG 4 cut(s) 51, 177, 273, 416
BsmAI GTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 562
BsmI GAATGC 1 cut(s) 583
BsnI GGCC 4 cut(s) 111, 239, 383, 421
BsoBI CYCGRG 2 cut(s) 24, 415
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 8 cut(s) 41, 75, 83, 136, 360, 467, 690, 752
Bsp1720I GCTNAGC 1 cut(s) 794
BspACI CCGC 1 cut(s) 488
BspANI GGCC 4 cut(s) 111, 239, 383, 421
BspCNI CTCAG 4 cut(s) 442, 643, 645, 748
BspDI ATCGAT 1 cut(s) 363
BspFNI CGCG 1 cut(s) 200
BspLI GGNNCC 4 cut(s) 85, 169, 420, 645
BspPI GGATC 5 cut(s) 36, 78, 91, 131, 475
BspT107I GGYRCC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 70
BsrFI RCCGGY 1 cut(s) 421
BsrI ACTGG 1 cut(s) 739
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 4 cut(s) 49, 192, 228, 674
BssMI GATC 8 cut(s) 41, 75, 83, 136, 360, 467, 690, 752
BssNI GRCGYC 1 cut(s) 224
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 569
Bst4CI ACNGT 1 cut(s) 460
BstACI GRCGYC 1 cut(s) 224
BstC8I GCNNGC 3 cut(s) 204, 407, 423
BstDEI CTNAG 5 cut(s) 450, 630, 653, 756, 794
BstDSI CCRYGG 1 cut(s) 228
BstF5I GGATG 2 cut(s) 60, 442
BstFNI CGCG 1 cut(s) 200
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 8 cut(s) 44, 78, 86, 139, 363, 470, 693, 755
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 8 cut(s) 41, 75, 83, 136, 360, 467, 690, 752
BstMCI CGRYCG 1 cut(s) 198
BstMWI GCNNNNNNNGC 3 cut(s) 197, 411, 520
BstNI CCWGG 1 cut(s) 569
BstNSI RCATGY 2 cut(s) 210, 485
BstSCI CCNGG 3 cut(s) 192, 523, 567
BstSLI GKGCMC 1 cut(s) 172
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 3 cut(s) 532, 573, 576
BstX2I RGATCY 1 cut(s) 83
BstYI RGATCY 1 cut(s) 83
Bsu15I ATCGAT 1 cut(s) 363
BsuRI GGCC 4 cut(s) 111, 239, 383, 421
BsuTUI ATCGAT 1 cut(s) 363
BtgI CCRYGG 1 cut(s) 228
BtsCI GGATG 2 cut(s) 60, 442
BtsI GCAGTG 2 cut(s) 18, 281
BtsIMutI CAGTG 2 cut(s) 18, 281
Cac8I GCNNGC 3 cut(s) 204, 407, 423
CfoI GCGC 1 cut(s) 202
Cfr10I RCCGGY 1 cut(s) 421
Cfr13I GGNCC 2 cut(s) 238, 419
ClaI ATCGAT 1 cut(s) 363
CseI GACGC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 31, 181
CviAII CATG 6 cut(s) 69, 188, 207, 299, 482, 596
CviQI GTAC 2 cut(s) 31, 181
DdeI CTNAG 5 cut(s) 450, 630, 653, 756, 794
DpnI GATC 8 cut(s) 43, 77, 85, 138, 362, 469, 692, 754
DpnII GATC 8 cut(s) 41, 75, 83, 136, 360, 467, 690, 752
EaeI YGGCCR 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 49
Eco57I CTGAAG 2 cut(s) 516, 686
Eco88I CYCGRG 2 cut(s) 24, 415
EcoRII CCWGG 1 cut(s) 567
EcoT14I CCWWGG 1 cut(s) 49
EcoT22I ATGCAT 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 6 cut(s) 72, 191, 210, 302, 485, 599
FalI AAGNNNNNCTT 1 cut(s) 782
FaqI GGGAC 1 cut(s) 562
FatI CATG 6 cut(s) 68, 187, 206, 298, 481, 595
FbaI TGATCA 1 cut(s) 690
Fnu4HI GCNGC 3 cut(s) 521, 562, 565
FokI GGATG 2 cut(s) 67, 449
Fsp4HI GCNGC 3 cut(s) 521, 562, 565
FspBI CTAG 4 cut(s) 582, 608, 635, 811
GlaI GCGC 1 cut(s) 201
GluI GCNGC 3 cut(s) 521, 562, 565
HaeIII GGCC 4 cut(s) 111, 239, 383, 421
HapII CCGG 5 cut(s) 194, 384, 422, 524, 722
HgaI GACGC 1 cut(s) 232
HhaI GCGC 1 cut(s) 202
Hin1I GRCGYC 1 cut(s) 224
Hin1II CATG 6 cut(s) 72, 191, 210, 302, 485, 599
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HindIII AAGCTT 2 cut(s) 698, 791
HpaII CCGG 5 cut(s) 194, 384, 422, 524, 722
HphI GGTGA 1 cut(s) 700
Hpy166II GTNNAC 1 cut(s) 740
Hpy188I TCNGA 7 cut(s) 136, 145, 313, 654, 695, 717, 757
Hpy188III TCNNGA 5 cut(s) 26, 160, 356, 664, 704
Hpy8I GTNNAC 1 cut(s) 740
HpyAV CCTTC 5 cut(s) 55, 150, 157, 373, 670
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4V TGCA 7 cut(s) 63, 68, 286, 479, 485, 514, 520
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 411, 520
HpyF3I CTNAG 5 cut(s) 450, 630, 653, 756, 794
Hsp92I GRCGYC 1 cut(s) 224
Hsp92II CATG 6 cut(s) 72, 191, 210, 302, 485, 599
HspAI GCGC 1 cut(s) 200
KroI GCCGGC 1 cut(s) 421
KroNI GCCGGC 1 cut(s) 423
Ksp22I TGATCA 1 cut(s) 690
Kzo9I GATC 8 cut(s) 41, 75, 83, 136, 360, 467, 690, 752
LmnI GCTCC 1 cut(s) 527
Lsp1109I GCAGC 3 cut(s) 532, 573, 576
LweI GCATC 1 cut(s) 507
MaeI CTAG 4 cut(s) 582, 608, 635, 811
MaeIII GTNAC 2 cut(s) 220, 766
MalI GATC 8 cut(s) 43, 77, 85, 138, 362, 469, 692, 754
MboI GATC 8 cut(s) 41, 75, 83, 136, 360, 467, 690, 752
MboII GAAGA 1 cut(s) 388
MflI RGATCY 1 cut(s) 83
MhlI GDGCHC 1 cut(s) 172
MluCI AATT 2 cut(s) 247, 729
MmeI TCCRAC 1 cut(s) 408
Mph1103I ATGCAT 1 cut(s) 70
MroNI GCCGGC 1 cut(s) 421
MseI TTAA 1 cut(s) 615
MspI CCGG 5 cut(s) 194, 384, 422, 524, 722
MspR9I CCNGG 3 cut(s) 194, 525, 569
Mva1269I GAATGC 1 cut(s) 583
MvaI CCWGG 1 cut(s) 569
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 197, 411, 520
NaeI GCCGGC 1 cut(s) 423
NciI CCSGG 2 cut(s) 194, 525
NdeII GATC 8 cut(s) 41, 75, 83, 136, 360, 467, 690, 752
NgoMIV GCCGGC 1 cut(s) 421
NlaIII CATG 6 cut(s) 72, 191, 210, 302, 485, 599
NlaIV GGNNCC 4 cut(s) 85, 169, 420, 645
NmeAIII GCCGAG 1 cut(s) 655
NmuCI GTSAC 2 cut(s) 220, 766
NsiI ATGCAT 1 cut(s) 70
NspI RCATGY 2 cut(s) 210, 485
PaeR7I CTCGAG 1 cut(s) 24
PcsI WCGNNNNNNNCGW 2 cut(s) 422, 748
PctI GAATGC 1 cut(s) 583
PdiI GCCGGC 1 cut(s) 423
PkrI GCNGC 3 cut(s) 522, 563, 566
Psp6I CCWGG 1 cut(s) 567
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 4 cut(s) 85, 169, 420, 645
PspPI GGNCC 2 cut(s) 238, 419
PsuI RGATCY 1 cut(s) 83
RsaI GTAC 2 cut(s) 32, 182
RsaNI GTAC 2 cut(s) 31, 181
SaqAI TTAA 1 cut(s) 615
SatI GCNGC 3 cut(s) 521, 562, 565
Sau3AI GATC 8 cut(s) 41, 75, 83, 136, 360, 467, 690, 752
Sau96I GGNCC 2 cut(s) 238, 419
ScrFI CCNGG 3 cut(s) 194, 525, 569
SduI GDGCHC 1 cut(s) 172
SfaNI GCATC 1 cut(s) 507
Sfr274I CTCGAG 1 cut(s) 24
SlaI CTCGAG 1 cut(s) 24
SmlI CTYRAG 1 cut(s) 24
SmoI CTYRAG 1 cut(s) 24
Sse9I AATT 2 cut(s) 247, 729
SsiI CCGC 1 cut(s) 488
SspMI CTAG 4 cut(s) 582, 608, 635, 811
StyD4I CCNGG 3 cut(s) 192, 523, 567
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 1 cut(s) 460
TaqI TCGA 4 cut(s) 25, 363, 539, 751
TaqII GACCGA 1 cut(s) 297
TasI AATT 2 cut(s) 247, 729
TatI WGTACW 1 cut(s) 30
Tru1I TTAA 1 cut(s) 615
Tru9I TTAA 1 cut(s) 615
TscAI CASTG 2 cut(s) 25, 288
TseFI GTSAC 2 cut(s) 220, 766
TseI GCWGC 3 cut(s) 520, 561, 564
Tsp45I GTSAC 2 cut(s) 220, 766
TspDTI ATGAA 3 cut(s) 115, 143, 584
TspGWI ACGGA 1 cut(s) 757
TspRI CASTG 2 cut(s) 25, 288
XapI RAATTY 1 cut(s) 247
XceI RCATGY 2 cut(s) 210, 485
XhoI CTCGAG 1 cut(s) 24
XspI CTAG 4 cut(s) 582, 608, 635, 811
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.