Rmu_sc0004727.1_g000003

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004727.1
OG0000187
Physical Location & Seq
Forward (+)
14531 .. 15379
849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004727.1_g000003.1.cds

Sequence Viewer

Length: 849 bp
atggtggggaagttgctcactgctcgggattataagatcaaatcccttcttgggatgttccgcaatgcatggcgaatcaatggatccctgcgggtggaggagacagacggtcagagggttctgttcactttgtcggatctttcggatcgagttcatatctggaagggtgctccgtgggggtacaatcatgcaccagtggcactggcatgttatgatggtgtgatgcccgtggaaaaggttcctctaaccatggcctcatactggataacactgcaaggtattccccctgcgtttcggtctgaaagagtgatgactatgattggttgcacacttggggattttcaggagatcgataagacagggaggaaggccagaaagcttcacattctggtagaaatctcgctgaacaagccgctgccatttaaacattggtattggctggaagatgaggttgaattcctctgcaaattcaagtattacaaactgtttggccagtgctccatctatggtttgatgacccatgtaggcttgccttgctcggggctgggattggaggaggatgatgatggggctgagaggacggtgttgggatcgcaaattgcacctacaccgtcggtgactttggctgaaggtatgcaaatgcagcacggagcttcggctttgagttcggggaagaagggaggcagcagctcggttttgaatgctggggttttgattcatggcaaactagtgtttaagacccctatccctcagccggttgtggaaactcctctgagctctttcttgaacaaacctcggcgacaagtggtgatcagagagctccaggaaactgctatggcaccagattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

282

Amino Acids

31.35

Weight (kDa)

8.72

Isoelectric Point (pI)

37.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000187)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20170 FvH4_4g06831
rosa_chinensis RchiOBHm_Chr1g0321991 RchiOBHm_Chr2g0161811
rosa_laevigata RLG00000015375 RLG00000018377
rosa_multiflora Rmu_co8011754.1_g000001 Rmu_co8019986.1_g000001 Rmu_co8067766.1_g000001 Rmu_co8156128.1_g000001 Rmu_co8219154.1_g000003 Rmu_co8297235.1_g000001 Rmu_co8345223.1_g000001 Rmu_co8364775.1_g000001 Rmu_co8465075.1_g000001 Rmu_sc0000007.1_g000002 Rmu_sc0000054.1_g000015 Rmu_sc0000054.1_g000023 Rmu_sc0000145.1_g000010 Rmu_sc0000166.1_g000006 Rmu_sc0000218.1_g000012 Rmu_sc0000339.1_g000008 Rmu_sc0000342.1_g000015 Rmu_sc0000464.1_g000008 Rmu_sc0000620.1_g000003 Rmu_sc0000638.1_g000017 Rmu_sc0000890.1_g000007 Rmu_sc0000894.1_g000024 Rmu_sc0000909.1_g000010 Rmu_sc0000928.1_g000020 Rmu_sc0000977.1_g000007 Rmu_sc0001022.1_g000003 Rmu_sc0001035.1_g000037 Rmu_sc0001060.1_g000008 Rmu_sc0001168.1_g000005 Rmu_sc0001173.1_g000022 Rmu_sc0001179.1_g000016 Rmu_sc0001408.1_g000003 Rmu_sc0001455.1_g000005 Rmu_sc0001484.1_g000008 Rmu_sc0001585.1_g000010 Rmu_sc0001702.1_g000030 Rmu_sc0001755.1_g000063 Rmu_sc0001903.1_g000009 Rmu_sc0002099.1_g000001 Rmu_sc0002142.1_g000012 Rmu_sc0002158.1_g000014 Rmu_sc0002307.1_g000009 Rmu_sc0002358.1_g000013 Rmu_sc0002421.1_g000002 Rmu_sc0002426.1_g000003 Rmu_sc0002438.1_g000001 Rmu_sc0002461.1_g000001 Rmu_sc0002508.1_g000003 Rmu_sc0002532.1_g000056 Rmu_sc0002583.1_g000002 Rmu_sc0002927.1_g000033 Rmu_sc0003033.1_g000040 Rmu_sc0003271.1_g000006 Rmu_sc0003381.1_g000006 Rmu_sc0003507.1_g000007 Rmu_sc0003578.1_g000003 Rmu_sc0003693.1_g000004 Rmu_sc0003693.1_g000005 Rmu_sc0003704.1_g000003 Rmu_sc0003840.1_g000002 Rmu_sc0004084.1_g000026 Rmu_sc0004193.1_g000007 Rmu_sc0004234.1_g000033 Rmu_sc0004235.1_g000025 Rmu_sc0004435.1_g000006 Rmu_sc0004727.1_g000003 Rmu_sc0004741.1_g000001 Rmu_sc0004926.1_g000004 Rmu_sc0005365.1_g000001 Rmu_sc0005392.1_g000007 Rmu_sc0005415.1_g000001 Rmu_sc0005539.1_g000007 Rmu_sc0005708.1_g000017 Rmu_sc0006317.1_g000007 Rmu_sc0006453.1_g000054 Rmu_sc0006536.1_g000004 Rmu_sc0006740.1_g000022 Rmu_sc0006763.1_g000012 Rmu_sc0007115.1_g000002 Rmu_sc0007115.1_g000010 Rmu_sc0007129.1_g000017 Rmu_sc0007131.1_g000010 Rmu_sc0008373.1_g000008 Rmu_sc0009005.1_g000008 Rmu_sc0009252.1_g000007 Rmu_sc0009599.1_g000022 Rmu_sc0009785.1_g000027 Rmu_sc0009891.1_g000003 Rmu_sc0010099.1_g000010 Rmu_sc0010266.1_g000008 Rmu_sc0010334.1_g000003 Rmu_sc0010381.1_g000001 Rmu_sc0010496.1_g000003 Rmu_sc0010496.1_g000004 Rmu_sc0010546.1_g000003 Rmu_sc0011482.1_g000006 Rmu_sc0011875.1_g000010 Rmu_sc0011917.1_g000006 Rmu_sc0012293.1_g000004 Rmu_sc0012847.1_g000003 Rmu_sc0013773.1_g000001 Rmu_sc0016060.1_g000003 Rmu_sc0016170.1_g000013 Rmu_sc0016393.1_g000002 Rmu_sc0017589.1_g000001 Rmu_sc0017786.1_g000005 Rmu_sc0018326.1_g000002 Rmu_sc0020760.1_g000001 Rmu_sc0021262.1_g000003 Rmu_sc0023851.1_g000002 Rmu_sc0024044.1_g000001 Rmu_sc0026820.1_g000001 Rmu_sc0032306.1_g000001 Rmu_sc0035666.1_g000001 Rmu_sc0036881.1_g000001 Rmu_sc0037030.1_g000001 Rmu_sc0037158.1_g000001 Rmu_ssc0000016.1_g000002 Rmu_ssc0000044.1_g000012 Rmu_ssc0000044.1_g000014 Rmu_ssc0000050.1_g000064 Rmu_ssc0000064.1_g000022 Rmu_ssc0000154.1_g000037 Rmu_ssc0000178.1_g000013 Rmu_ssc0000217.1_g000022 Rmu_ssc0000392.1_g000004
rosa_roxburghii Rroxscaffold_3G00242000 Rroxscaffold_3G00242530 Rroxscaffold_6G00406010
rosa_rugosa Rorug01G0062000 Rorug02G0224000 Rorug02G0262900 Rorug02G0263000 Rorug02G0358900 Rorug02G0360500 Rorug02G0378500.1 Rorug02G0378600 Rorug02G0394700 Rorug03G0122900 Rorug03G0254600 Rorug03G0254700 Rorug03G0254800 Rorug04G0042200.1 Rorug04G0378100 Rorug05G0053400 Rorug05G0238600 Rorug05G0364500 Rorug05G0562000 Rorug06G0089300 Rorug06G0089400 Rorug06G0145700 Rorug07G0111600 Rorug07G0229500.1 Rorug07G0287600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 33
AccB1I GGYRCC 1 cut(s) 838
AciI CCGC 3 cut(s) 61, 91, 413
AclWI GGATC 5 cut(s) 78, 91, 144, 153, 598
AcoI YGGCCR 1 cut(s) 490
AcsI RAATTY 2 cut(s) 455, 467
AcuI CTGAAG 1 cut(s) 648
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 5 cut(s) 51, 94, 261, 539, 754
AgsI TTSAA 4 cut(s) 455, 472, 700, 787
AhdI GACNNNNNGTC 1 cut(s) 108
AhlI ACTAGT 1 cut(s) 727
AjnI CCWGG 1 cut(s) 822
AluBI AGCT 5 cut(s) 379, 653, 690, 777, 820
AluI AGCT 5 cut(s) 379, 653, 690, 777, 820
Alw21I GWGCWC 4 cut(s) 172, 500, 779, 822
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 5 cut(s) 78, 91, 144, 153, 598
Ama87I CYCGRG 2 cut(s) 24, 538
AoxI GGCC 3 cut(s) 252, 369, 490
ApeKI GCWGC 4 cut(s) 415, 643, 684, 687
ApoI RAATTY 2 cut(s) 455, 467
Asp700I GAANNNNTTC 1 cut(s) 237
AsuHPI GGTGA 2 cut(s) 628, 820
AvaI CYCGRG 2 cut(s) 24, 538
BalI TGGCCA 1 cut(s) 492
BamHI GGATCC 1 cut(s) 83
BanI GGYRCC 1 cut(s) 838
BanII GRGCYC 2 cut(s) 779, 822
Bbv12I GWGCWC 4 cut(s) 172, 500, 779, 822
BbvCI CCTCAGC 1 cut(s) 750
BbvI GCAGC 4 cut(s) 402, 655, 696, 699
BccI CCATC 3 cut(s) 209, 509, 560
BciT130I CCWGG 1 cut(s) 824
BclI TGATCA 1 cut(s) 810
BcoDI GTCTC 1 cut(s) 95
BcuI ACTAGT 1 cut(s) 727
BfaI CTAG 1 cut(s) 728
BisI GCNGC 5 cut(s) 413, 416, 644, 685, 688
BlsI GCNGC 5 cut(s) 414, 417, 645, 686, 689
Bme1390I CCNGG 1 cut(s) 824
BmeRI GACNNNNNGTC 1 cut(s) 108
BmeT110I CYCGRG 2 cut(s) 24, 538
BmiI GGNNCC 3 cut(s) 85, 240, 840
BmrFI CCNGG 1 cut(s) 824
BmsI GCATC 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 806
Bpu10I CCTNAGC 1 cut(s) 750
Bsa29I ATCGAT 1 cut(s) 351
BsaJI CCNNGG 4 cut(s) 173, 228, 249, 794
Bsc4I CCNNNNNNNGG 5 cut(s) 51, 94, 261, 539, 754
Bse118I RCCGGY 1 cut(s) 754
Bse1I ACTGG 4 cut(s) 194, 207, 266, 493
Bse3DI GCAATG 1 cut(s) 70
BseBI CCWGG 1 cut(s) 824
BseCI ATCGAT 1 cut(s) 351
BseDI CCNNGG 4 cut(s) 173, 228, 249, 794
BseGI GGATG 2 cut(s) 60, 565
BseLI CCNNNNNNNGG 5 cut(s) 51, 94, 261, 539, 754
BseMI GCAATG 1 cut(s) 70
BseMII CTCAG 3 cut(s) 564, 764, 764
BseNI ACTGG 4 cut(s) 194, 207, 266, 493
BseRI GAGGAG 3 cut(s) 113, 569, 759
BseXI GCAGC 4 cut(s) 402, 655, 696, 699
BseYI CCCAGC 2 cut(s) 544, 704
BshFI GGCC 3 cut(s) 254, 371, 492
BshNI GGYRCC 1 cut(s) 838
BshVI ATCGAT 1 cut(s) 351
BsiHKAI GWGCWC 4 cut(s) 172, 500, 779, 822
BsiHKCI CYCGRG 2 cut(s) 24, 538
BsiSI CCGG 1 cut(s) 755
BslI CCNNNNNNNGG 5 cut(s) 51, 94, 261, 539, 754
BsmAI GTCTC 1 cut(s) 95
BsmI GAATGC 1 cut(s) 706
BsnI GGCC 3 cut(s) 254, 371, 492
BsoBI CYCGRG 2 cut(s) 24, 538
Bsp1286I GDGCHC 4 cut(s) 172, 500, 779, 822
Bsp143I GATC 7 cut(s) 36, 83, 136, 145, 348, 590, 810
Bsp19I CCATGG 1 cut(s) 249
BspACI CCGC 3 cut(s) 61, 91, 413
BspANI GGCC 3 cut(s) 254, 371, 492
BspCNI CTCAG 3 cut(s) 565, 763, 765
BspDI ATCGAT 1 cut(s) 351
BspLI GGNNCC 3 cut(s) 85, 240, 840
BspPI GGATC 5 cut(s) 78, 91, 144, 153, 598
BspT107I GGYRCC 1 cut(s) 838
BsrDI GCAATG 1 cut(s) 70
BsrFI RCCGGY 1 cut(s) 754
BsrI ACTGG 4 cut(s) 194, 207, 266, 493
BssAI RCCGGY 1 cut(s) 754
BssECI CCNNGG 4 cut(s) 173, 228, 249, 794
BssMI GATC 7 cut(s) 36, 83, 136, 145, 348, 590, 810
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 824
Bst4CI ACNGT 4 cut(s) 110, 486, 583, 612
BstC8I GCNNGC 1 cut(s) 530
BstDEI CTNAG 3 cut(s) 573, 750, 773
BstDSI CCRYGG 3 cut(s) 173, 228, 249
BstF5I GGATG 2 cut(s) 60, 565
BstKTI GATC 7 cut(s) 39, 86, 139, 148, 351, 593, 813
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 7 cut(s) 36, 83, 136, 145, 348, 590, 810
BstMWI GCNNNNNNNGC 4 cut(s) 197, 409, 534, 643
BstNI CCWGG 1 cut(s) 824
BstNSI RCATGY 1 cut(s) 210
BstSCI CCNGG 1 cut(s) 822
BstV1I GCAGC 4 cut(s) 402, 655, 696, 699
BstX2I RGATCY 2 cut(s) 83, 136
BstYI RGATCY 2 cut(s) 83, 136
Bsu15I ATCGAT 1 cut(s) 351
BsuRI GGCC 3 cut(s) 254, 371, 492
BsuTUI ATCGAT 1 cut(s) 351
BtgI CCRYGG 3 cut(s) 173, 228, 249
BtsCI GGATG 2 cut(s) 60, 565
BtsI GCAGTG 2 cut(s) 18, 269
BtsIMutI CAGTG 5 cut(s) 18, 200, 201, 269, 500
Cac8I GCNNGC 1 cut(s) 530
Cfr10I RCCGGY 1 cut(s) 754
ClaI ATCGAT 1 cut(s) 351
Csp6I GTAC 1 cut(s) 181
CviAII CATG 6 cut(s) 69, 188, 207, 250, 521, 719
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 3 cut(s) 573, 750, 773
DpnI GATC 7 cut(s) 38, 85, 138, 147, 350, 592, 812
DpnII GATC 7 cut(s) 36, 83, 136, 145, 348, 590, 810
DraI TTTAAA 1 cut(s) 424
DriI GACNNNNNGTC 1 cut(s) 108
EaeI YGGCCR 1 cut(s) 490
Eam1105I GACNNNNNGTC 1 cut(s) 108
Ecl136II GAGCTC 2 cut(s) 777, 820
Eco130I CCWWGG 1 cut(s) 249
Eco24I GRGCYC 2 cut(s) 779, 822
Eco53kI GAGCTC 2 cut(s) 777, 820
Eco57I CTGAAG 1 cut(s) 648
Eco88I CYCGRG 2 cut(s) 24, 538
EcoICRI GAGCTC 2 cut(s) 777, 820
EcoRI GAATTC 1 cut(s) 455
EcoRII CCWGG 1 cut(s) 822
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 70
EcoT38I GRGCYC 2 cut(s) 779, 822
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 6 cut(s) 72, 191, 210, 253, 524, 722
FatI CATG 6 cut(s) 68, 187, 206, 249, 520, 718
FauI CCCGC 1 cut(s) 84
FbaI TGATCA 1 cut(s) 810
Fnu4HI GCNGC 5 cut(s) 413, 416, 644, 685, 688
FokI GGATG 2 cut(s) 67, 572
FriOI GRGCYC 2 cut(s) 779, 822
Fsp4HI GCNGC 5 cut(s) 413, 416, 644, 685, 688
FspBI CTAG 1 cut(s) 728
GluI GCNGC 5 cut(s) 413, 416, 644, 685, 688
GsaI CCCAGC 2 cut(s) 548, 708
GsuI CTGGAG 1 cut(s) 806
HaeIII GGCC 3 cut(s) 254, 371, 492
HapII CCGG 1 cut(s) 755
Hin1II CATG 6 cut(s) 72, 191, 210, 253, 524, 722
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 2 cut(s) 75, 715
HpaII CCGG 1 cut(s) 755
HphI GGTGA 2 cut(s) 628, 820
Hpy166II GTNNAC 1 cut(s) 126
Hpy188I TCNGA 6 cut(s) 114, 136, 145, 301, 774, 815
Hpy188III TCNNGA 4 cut(s) 26, 160, 344, 784
Hpy8I GTNNAC 1 cut(s) 126
Hpy99I CGWCG 1 cut(s) 616
HpyAV CCTTC 5 cut(s) 56, 157, 361, 623, 670
HpyCH4III ACNGT 4 cut(s) 110, 486, 583, 612
HpyCH4V TGCA 8 cut(s) 68, 191, 274, 327, 465, 602, 637, 643
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 409, 534, 643
HpyF3I CTNAG 3 cut(s) 573, 750, 773
Hsp92II CATG 6 cut(s) 72, 191, 210, 253, 524, 722
Ksp22I TGATCA 1 cut(s) 810
Kzo9I GATC 7 cut(s) 36, 83, 136, 145, 348, 590, 810
LmnI GCTCC 4 cut(s) 175, 503, 650, 825
Lsp1109I GCAGC 4 cut(s) 402, 655, 696, 699
LweI GCATC 1 cut(s) 213
MaeI CTAG 1 cut(s) 728
MaeIII GTNAC 1 cut(s) 616
MalI GATC 7 cut(s) 38, 85, 138, 147, 350, 592, 812
MboI GATC 7 cut(s) 36, 83, 136, 145, 348, 590, 810
MboII GAAGA 2 cut(s) 455, 685
MflI RGATCY 2 cut(s) 83, 136
MhlI GDGCHC 4 cut(s) 172, 500, 779, 822
MlsI TGGCCA 1 cut(s) 492
MluCI AATT 3 cut(s) 455, 467, 597
MluNI TGGCCA 1 cut(s) 492
MmeI TCCRAC 1 cut(s) 114
Mox20I TGGCCA 1 cut(s) 492
Mph1103I ATGCAT 1 cut(s) 70
MroXI GAANNNNTTC 1 cut(s) 237
MscI TGGCCA 1 cut(s) 492
MseI TTAA 2 cut(s) 423, 735
MslI CAYNNNNRTG 1 cut(s) 205
Msp20I TGGCCA 1 cut(s) 492
MspA1I CMGCKG 1 cut(s) 415
MspI CCGG 1 cut(s) 755
MspR9I CCNGG 1 cut(s) 824
Mva1269I GAATGC 1 cut(s) 706
MvaI CCWGG 1 cut(s) 824
MwoI GCNNNNNNNGC 4 cut(s) 197, 409, 534, 643
NcoI CCATGG 1 cut(s) 249
NdeII GATC 7 cut(s) 36, 83, 136, 145, 348, 590, 810
NlaIII CATG 6 cut(s) 72, 191, 210, 253, 524, 722
NlaIV GGNNCC 3 cut(s) 85, 240, 840
NmeAIII GCCGAG 1 cut(s) 775
NmuCI GTSAC 1 cut(s) 616
NsiI ATGCAT 1 cut(s) 70
NspI RCATGY 1 cut(s) 210
PctI GAATGC 1 cut(s) 706
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 2 cut(s) 75, 715
PfoI TCCNGGA 1 cut(s) 822
PkrI GCNGC 5 cut(s) 414, 417, 645, 686, 689
PsiI TTATAA 1 cut(s) 33
Psp124BI GAGCTC 2 cut(s) 779, 822
Psp6I CCWGG 1 cut(s) 822
PspFI CCCAGC 2 cut(s) 544, 704
PspGI CCWGG 1 cut(s) 822
PspN4I GGNNCC 3 cut(s) 85, 240, 840
PsuI RGATCY 2 cut(s) 83, 136
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
RseI CAYNNNNRTG 1 cut(s) 205
SacI GAGCTC 2 cut(s) 779, 822
SaqAI TTAA 2 cut(s) 423, 735
SatI GCNGC 5 cut(s) 413, 416, 644, 685, 688
Sau3AI GATC 7 cut(s) 36, 83, 136, 145, 348, 590, 810
ScrFI CCNGG 1 cut(s) 824
SduI GDGCHC 4 cut(s) 172, 500, 779, 822
SfaNI GCATC 1 cut(s) 213
SmiMI CAYNNNNRTG 1 cut(s) 205
SpeI ACTAGT 1 cut(s) 727
Sse9I AATT 3 cut(s) 455, 467, 597
SsiI CCGC 3 cut(s) 61, 91, 413
SspMI CTAG 1 cut(s) 728
SstI GAGCTC 2 cut(s) 779, 822
StyD4I CCNGG 1 cut(s) 822
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 4 cut(s) 110, 486, 583, 612
TaqI TCGA 2 cut(s) 148, 351
TaqII GACCGA 1 cut(s) 285
TasI AATT 3 cut(s) 455, 467, 597
TauI GCSGC 1 cut(s) 415
TfiI GAWTC 2 cut(s) 75, 715
Tru1I TTAA 2 cut(s) 423, 735
Tru9I TTAA 2 cut(s) 423, 735
TscAI CASTG 5 cut(s) 25, 201, 207, 276, 500
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 4 cut(s) 415, 643, 684, 687
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 2 cut(s) 143, 707
TspGWI ACGGA 2 cut(s) 162, 663
TspRI CASTG 5 cut(s) 25, 201, 207, 276, 500
XapI RAATTY 2 cut(s) 455, 467
XceI RCATGY 1 cut(s) 210
XcmI CCANNNNNNNNNTGG 1 cut(s) 426
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 1 cut(s) 728
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.