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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 4 cut(s) | 16, 66, 76, 82 |
| AjnI | CCWGG | 1 cut(s) | 49 |
| AluBI | AGCT | 2 cut(s) | 19, 130 |
| AluI | AGCT | 2 cut(s) | 19, 130 |
| AsuHPI | GGTGA | 1 cut(s) | 97 |
| BciT130I | CCWGG | 1 cut(s) | 51 |
| BfaI | CTAG | 1 cut(s) | 172 |
| Bme1390I | CCNGG | 1 cut(s) | 51 |
| BmrFI | CCNGG | 1 cut(s) | 51 |
| BsaJI | CCNNGG | 1 cut(s) | 133 |
| BseBI | CCWGG | 1 cut(s) | 51 |
| BseDI | CCNNGG | 1 cut(s) | 133 |
| BseMII | CTCAG | 1 cut(s) | 42 |
| BspCNI | CTCAG | 1 cut(s) | 41 |
| BssECI | CCNNGG | 1 cut(s) | 133 |
| BssT1I | CCWWGG | 1 cut(s) | 133 |
| Bst2UI | CCWGG | 1 cut(s) | 51 |
| Bst4CI | ACNGT | 1 cut(s) | 12 |
| BstDEI | CTNAG | 1 cut(s) | 28 |
| BstNI | CCWGG | 1 cut(s) | 51 |
| BstNSI | RCATGY | 1 cut(s) | 46 |
| BstSCI | CCNGG | 1 cut(s) | 49 |
| CviAII | CATG | 2 cut(s) | 43, 58 |
| CviJI | RGCY | 4 cut(s) | 19, 54, 130, 169 |
| CviKI_1 | RGCY | 4 cut(s) | 19, 54, 130, 169 |
| DdeI | CTNAG | 1 cut(s) | 28 |
| Eco130I | CCWWGG | 1 cut(s) | 133 |
| EcoRII | CCWGG | 1 cut(s) | 49 |
| EcoT14I | CCWWGG | 1 cut(s) | 133 |
| ErhI | CCWWGG | 1 cut(s) | 133 |
| FaeI | CATG | 2 cut(s) | 46, 61 |
| FaiI | YATR | 2 cut(s) | 44, 59 |
| FatI | CATG | 2 cut(s) | 42, 57 |
| FspBI | CTAG | 1 cut(s) | 172 |
| Hin1II | CATG | 2 cut(s) | 46, 61 |
| HinfI | GANTC | 3 cut(s) | 88, 140, 146 |
| HphI | GGTGA | 1 cut(s) | 97 |
| Hpy188I | TCNGA | 3 cut(s) | 31, 145, 159 |
| Hpy188III | TCNNGA | 1 cut(s) | 103 |
| HpyAV | CCTTC | 2 cut(s) | 28, 76 |
| HpyCH4III | ACNGT | 1 cut(s) | 12 |
| HpyCH4V | TGCA | 1 cut(s) | 46 |
| HpyF3I | CTNAG | 1 cut(s) | 28 |
| Hsp92II | CATG | 2 cut(s) | 46, 61 |
| LmnI | GCTCC | 1 cut(s) | 135 |
| LpnPI | CCDG | 3 cut(s) | 36, 63, 101 |
| MaeI | CTAG | 1 cut(s) | 172 |
| MboII | GAAGA | 1 cut(s) | 91 |
| MluCI | AATT | 1 cut(s) | 77 |
| MnlI | CCTC | 1 cut(s) | 37 |
| MseI | TTAA | 1 cut(s) | 164 |
| MspR9I | CCNGG | 1 cut(s) | 51 |
| MvaI | CCWGG | 1 cut(s) | 51 |
| NlaIII | CATG | 2 cut(s) | 46, 61 |
| NspI | RCATGY | 1 cut(s) | 46 |
| PfeI | GAWTC | 3 cut(s) | 88, 140, 146 |
| Psp6I | CCWGG | 1 cut(s) | 49 |
| PspGI | CCWGG | 1 cut(s) | 49 |
| SaqAI | TTAA | 1 cut(s) | 164 |
| ScrFI | CCNGG | 1 cut(s) | 51 |
| SetI | ASST | 4 cut(s) | 21, 52, 87, 132 |
| SgeI | CNNG | 9 cut(s) | 34, 55, 62, 63, 70, 115, 128, 139, 146 |
| Sse9I | AATT | 1 cut(s) | 77 |
| SspMI | CTAG | 1 cut(s) | 172 |
| StyD4I | CCNGG | 1 cut(s) | 49 |
| StyI | CCWWGG | 1 cut(s) | 133 |
| TaaI | ACNGT | 1 cut(s) | 12 |
| TaqI | TCGA | 1 cut(s) | 104 |
| TasI | AATT | 1 cut(s) | 77 |
| TfiI | GAWTC | 3 cut(s) | 88, 140, 146 |
| Tru1I | TTAA | 1 cut(s) | 164 |
| Tru9I | TTAA | 1 cut(s) | 164 |
| TspDTI | ATGAA | 2 cut(s) | 46, 138 |
| XceI | RCATGY | 1 cut(s) | 46 |
| XspI | CTAG | 1 cut(s) | 172 |