Rorug05G0238600

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000187
Physical Location & Seq
Reverse (-)
25259939 .. 25263200
3262 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0238600.1

Sequence Viewer

Length: 2433 bp
ATGCAACTGGGTGGTGTTACTTTGTTTTTCTTATTCATCTTCAGTTTGCTTCCTTCACAGTATGGCGCTGAAGTATATAACATAACTCCTTCACACCCATTAGCAGAGGGACAAACTCTAGTCTCTCCTGGCCTAATATTTGAACTGGGCTTCTTCAATCCTAATAGTTCTGCCAATAAGTATGTGGGCTTGTGGCACAAAAGTATATATCCCCGTAAATATGTGTGGGTGGCTAACAGAGATAATCCTCTTGCGGCTAGAGACACCTTCGTTAGTTTGAGAATTGGCAGCAGCGGGAGTCTGGAGCTCGTAGATGGGAAACAGATTTCTGTCTGGTCCGCCAATATATCCAATTGTTCATCTGCAGTTCTCTTGGATAATGGAAACTTTGTTGTCAAAGATGATATGGGAGCTGATATATGGGAGAGTTTCAATAATCCTAGTGACTCACAACTGCCAAGCATGATGGGAAAACAGATTTTCTACACATCTTGGAGAAGTGATAATGATCCATCAACGGGGATATTCAAGGCCGGATATTCAGCAGAGTTTCCATCACAATCGTTCATTTGGATTAATGGATCTATTCCCTACTGGAGAAGTGGGCCTTGGGATCAATCAAAGTATATTGGTGTGACACCGGGAACAAGGTATAACTCTTACAGTTTTAACAAAATTCCAGGTGACAAAGTTTTTACATATATAGACATATCTTCAGAAGGAGTAATGAGGAAAATGCTTTCAGAAAGTGGGAAGAACTGGTATCTTGACTGGGAGTCATGGAAAAACCCATGCGATAATTATGGCGCCTGTGGACCTTTTGGGGTTTGCAATGCTTCTGACTCTCCAATCTGCAAGTGTTTGAAAGGGTTTATACCCAAGTCAAATGAAGAATGGAGCAAAAGAAACTGGAGCCGAGGTTGTGTGAGACGAACGAATTTGTCTTGTGAGACTAACACAAATGATTCAGTCTCATCAAAAGGAAAAGATGGGTTTTTGAAGTTGGAAAGAGTGAAAGTACCCGATTTTCATGAATATCTGAATTCTGTGTCTCTAGACAAGCTCGAGGACTGCAAGATACAGTGCCTGAGTAATTGTTCTTGCCTGGCTTATGCATATGTTGATACCATAGGGTGTTTGGTCTGGTCCAAAGACCTTATTGATATGCAGAAGTTTCCATCTCTTGGAGAAGATCTTCACGTCCGCCTAGCACCCTCAGAACTAGATGAAGAAAAGCCGATAAAGTTAATTGCTAGCCTTACAGCTATTGGGTTTATGAGTATCTTGATTGCAATAGTCTTCAGTTTGCACAGGTGGCGTGCCAACCAAAAGCGACACGTTGGATTAACAGCACGACGCTTGGAAACAACTAGTATGATTAAGATTCATAGAGATGGTCTTCGAGAATATATAGGACAACATGATTTCTCGGAGCTAAAGATATATGATTTTGATAGCATATCGATCGCCACAGACAACTTCAGCATAACAAACAAACTCGGTCAAGGAGGCTTTGGCCCAGTTTATAAGGGGATGCTACCGGAAGGGAAGGAAATAGCAGTAAAAAGACTATCTAGTAGCTCAAGACAAGGAGTTGAAGAGTTCAAGAATGAGATGCTGTTGATCTCCAATCTTCAACACAAAAACCTTGTTAGGATGATGGGTTGCTGTGTTAAAGAGAATGAGAAGTTACTGATCTACGAGTTCATGCCAAACAAAAGCTTGGATACTTTTCTATATGATCCGACAAAGAGAGCAATGCTTGATTGGGCTTCACGCTTTAATATTATTCAGGGTGTTGCTAGAGGGCTTCTTTATCTCCATCATGATTCCTATGTGAAGGTGATACATAGAGATTTAAAAGTCAGTAACATTCTCTTAGATGAGAAATTGAATCCAAAAATCTCAGATTTTGGATTGGCACGCATAATTGAAGAAACACAAAGTCAAGAAAATACTCAGAAGGTTGTTGGAACACGTGGCTATATGTCTCCAGAGTATGCCATGGGTGGGATATTTTCTGAAAAATCTGATGTCTACAGCTTTGGAGTCCTGGTATTAGAGATTATAAGCAGCAAGAAGAATACCAGCTTCTATTTTTATGATCAACAGCTAGGCTTCCTTTCCTATGCATGGAAGTTGTGGAATGAAGGCAGGGCGTTGGAGTTTGTAGATGAAGTATTGGGTGATTCATATTCCTCATCCGAAGTAATGAGATGTGTGCATGTTGGGCTTCTTTGTGTACAGGACAATGCTACGGATAGACCAACCATGACGGATGTAGCTTCAATTCTAAGCAGCGAGAAAGATGGTCCACATCCGAAGAGGCCTGTATTCACTACCCAAAACTCAGTTTATCATCCTGGACCATACTATGAAAATACAAATTCCTCAAAAAATGAAGCTAGCATTACAATAATTGAAGGACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

810

Amino Acids

91.41

Weight (kDa)

6.47

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 156 4.4e-19 D-mannose binding lectin
S_locus_glycop PF00954 199 - 295 5e-20 S-locus glycoprotein domain
PAN_2 PF08276 326 - 389 1.2e-18 PAN-like domain
Pkinase PF00069 495 - 695 7.6e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 496 - 763 1.1e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000187)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20170 FvH4_4g06831
rosa_chinensis RchiOBHm_Chr1g0321991 RchiOBHm_Chr2g0161811
rosa_laevigata RLG00000015375 RLG00000018377
rosa_multiflora Rmu_co8011754.1_g000001 Rmu_co8019986.1_g000001 Rmu_co8067766.1_g000001 Rmu_co8156128.1_g000001 Rmu_co8219154.1_g000003 Rmu_co8297235.1_g000001 Rmu_co8345223.1_g000001 Rmu_co8364775.1_g000001 Rmu_co8465075.1_g000001 Rmu_sc0000007.1_g000002 Rmu_sc0000054.1_g000015 Rmu_sc0000054.1_g000023 Rmu_sc0000145.1_g000010 Rmu_sc0000166.1_g000006 Rmu_sc0000218.1_g000012 Rmu_sc0000339.1_g000008 Rmu_sc0000342.1_g000015 Rmu_sc0000464.1_g000008 Rmu_sc0000620.1_g000003 Rmu_sc0000638.1_g000017 Rmu_sc0000890.1_g000007 Rmu_sc0000894.1_g000024 Rmu_sc0000909.1_g000010 Rmu_sc0000928.1_g000020 Rmu_sc0000977.1_g000007 Rmu_sc0001022.1_g000003 Rmu_sc0001035.1_g000037 Rmu_sc0001060.1_g000008 Rmu_sc0001168.1_g000005 Rmu_sc0001173.1_g000022 Rmu_sc0001179.1_g000016 Rmu_sc0001408.1_g000003 Rmu_sc0001455.1_g000005 Rmu_sc0001484.1_g000008 Rmu_sc0001585.1_g000010 Rmu_sc0001702.1_g000030 Rmu_sc0001755.1_g000063 Rmu_sc0001903.1_g000009 Rmu_sc0002099.1_g000001 Rmu_sc0002142.1_g000012 Rmu_sc0002158.1_g000014 Rmu_sc0002307.1_g000009 Rmu_sc0002358.1_g000013 Rmu_sc0002421.1_g000002 Rmu_sc0002426.1_g000003 Rmu_sc0002438.1_g000001 Rmu_sc0002461.1_g000001 Rmu_sc0002508.1_g000003 Rmu_sc0002532.1_g000056 Rmu_sc0002583.1_g000002 Rmu_sc0002927.1_g000033 Rmu_sc0003033.1_g000040 Rmu_sc0003271.1_g000006 Rmu_sc0003381.1_g000006 Rmu_sc0003507.1_g000007 Rmu_sc0003578.1_g000003 Rmu_sc0003693.1_g000004 Rmu_sc0003693.1_g000005 Rmu_sc0003704.1_g000003 Rmu_sc0003840.1_g000002 Rmu_sc0004084.1_g000026 Rmu_sc0004193.1_g000007 Rmu_sc0004234.1_g000033 Rmu_sc0004235.1_g000025 Rmu_sc0004435.1_g000006 Rmu_sc0004727.1_g000003 Rmu_sc0004741.1_g000001 Rmu_sc0004926.1_g000004 Rmu_sc0005365.1_g000001 Rmu_sc0005392.1_g000007 Rmu_sc0005415.1_g000001 Rmu_sc0005539.1_g000007 Rmu_sc0005708.1_g000017 Rmu_sc0006317.1_g000007 Rmu_sc0006453.1_g000054 Rmu_sc0006536.1_g000004 Rmu_sc0006740.1_g000022 Rmu_sc0006763.1_g000012 Rmu_sc0007115.1_g000002 Rmu_sc0007115.1_g000010 Rmu_sc0007129.1_g000017 Rmu_sc0007131.1_g000010 Rmu_sc0008373.1_g000008 Rmu_sc0009005.1_g000008 Rmu_sc0009252.1_g000007 Rmu_sc0009599.1_g000022 Rmu_sc0009785.1_g000027 Rmu_sc0009891.1_g000003 Rmu_sc0010099.1_g000010 Rmu_sc0010266.1_g000008 Rmu_sc0010334.1_g000003 Rmu_sc0010381.1_g000001 Rmu_sc0010496.1_g000003 Rmu_sc0010496.1_g000004 Rmu_sc0010546.1_g000003 Rmu_sc0011482.1_g000006 Rmu_sc0011875.1_g000010 Rmu_sc0011917.1_g000006 Rmu_sc0012293.1_g000004 Rmu_sc0012847.1_g000003 Rmu_sc0013773.1_g000001 Rmu_sc0016060.1_g000003 Rmu_sc0016170.1_g000013 Rmu_sc0016393.1_g000002 Rmu_sc0017589.1_g000001 Rmu_sc0017786.1_g000005 Rmu_sc0018326.1_g000002 Rmu_sc0020760.1_g000001 Rmu_sc0021262.1_g000003 Rmu_sc0023851.1_g000002 Rmu_sc0024044.1_g000001 Rmu_sc0026820.1_g000001 Rmu_sc0032306.1_g000001 Rmu_sc0035666.1_g000001 Rmu_sc0036881.1_g000001 Rmu_sc0037030.1_g000001 Rmu_sc0037158.1_g000001 Rmu_ssc0000016.1_g000002 Rmu_ssc0000044.1_g000012 Rmu_ssc0000044.1_g000014 Rmu_ssc0000050.1_g000064 Rmu_ssc0000064.1_g000022 Rmu_ssc0000154.1_g000037 Rmu_ssc0000178.1_g000013 Rmu_ssc0000217.1_g000022 Rmu_ssc0000392.1_g000004
rosa_roxburghii Rroxscaffold_3G00242000 Rroxscaffold_3G00242530 Rroxscaffold_6G00406010
rosa_rugosa Rorug01G0062000 Rorug02G0224000 Rorug02G0262900 Rorug02G0263000 Rorug02G0358900 Rorug02G0360500 Rorug02G0378500.1 Rorug02G0378600 Rorug02G0394700 Rorug03G0122900 Rorug03G0254600 Rorug03G0254700 Rorug03G0254800 Rorug04G0042200.1 Rorug04G0378100 Rorug05G0053400 Rorug05G0238600 Rorug05G0364500 Rorug05G0562000 Rorug06G0089300 Rorug06G0089400 Rorug06G0145700 Rorug07G0111600 Rorug07G0229500.1 Rorug07G0287600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1527, 2069
AccB1I GGYRCC 1 cut(s) 806
AccB7I CCANNNNNTGG 1 cut(s) 1184
AccI GTMKAC 1 cut(s) 2037
AciI CCGC 4 cut(s) 254, 294, 339, 1204
AclWI GGATC 4 cut(s) 503, 589, 621, 1736
AcsI RAATTY 4 cut(s) 675, 937, 1042, 2386
AcuI CTGAAG 5 cut(s) 25, 90, 699, 1285, 1465
AcvI CACGTG 1 cut(s) 1979
AcyI GRCGYC 1 cut(s) 807
AfaI GTAC 2 cut(s) 1020, 2244
AfiI CCNNNNNNNGG 4 cut(s) 518, 1184, 2010, 2133
AflIII ACRYGT 2 cut(s) 1336, 1976
AhdI GACNNNNNGTC 1 cut(s) 775
AhlI ACTAGT 1 cut(s) 1370
AjiI CACGTC 1 cut(s) 1201
AjnI CCWGG 5 cut(s) 127, 679, 1104, 2052, 2362
AjuI GAANNNNNNNTTGG 2 cut(s) 592, 624
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 7 cut(s) 127, 255, 922, 944, 976, 1056, 1995
AlwI GGATC 4 cut(s) 503, 589, 621, 1736
AlwNI CAGNNNCTG 1 cut(s) 1087
Ama87I CYCGRG 1 cut(s) 1064
AoxI GGCC 5 cut(s) 130, 531, 605, 1516, 2327
ApeKI GCWGC 4 cut(s) 288, 291, 2073, 2298
ApoI RAATTY 4 cut(s) 675, 937, 1042, 2386
AseI ATTAAT 1 cut(s) 576
Asp700I GAANNNNTTC 1 cut(s) 1194
AspLEI GCGC 2 cut(s) 68, 809
AspS9I GGNCC 7 cut(s) 336, 605, 815, 1146, 1517, 2312, 2366
AsuC2I CCSGG 1 cut(s) 642
AsuHPI GGTGA 3 cut(s) 695, 1855, 2198
AsuNHI GCTAGC 2 cut(s) 1253, 2405
AvaI CYCGRG 1 cut(s) 1064
AvaII GGWCC 5 cut(s) 336, 815, 1146, 2312, 2366
BanI GGYRCC 1 cut(s) 806
BanII GRGCYC 1 cut(s) 309
BbrPI CACGTG 1 cut(s) 1979
BbsI GAAGAC 2 cut(s) 1291, 1391
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 4 cut(s) 300, 303, 2085, 2310
BcgI CGANNNNNNTGC 2 cut(s) 1447, 1481
BciT130I CCWGG 5 cut(s) 129, 681, 1106, 2054, 2364
BciVI GTATCC 1 cut(s) 1720
BclI TGATCA 1 cut(s) 2104
BcnI CCSGG 1 cut(s) 642
BcoDI GTCTC 7 cut(s) 127, 255, 922, 944, 976, 1056, 1995
BcuI ACTAGT 1 cut(s) 1370
BfmI CTRYAG 2 cut(s) 363, 2038
BfoI RGCGCY 2 cut(s) 69, 810
BfuI GTATCC 1 cut(s) 1720
BglII AGATCT 1 cut(s) 1192
BisI GCNGC 5 cut(s) 255, 289, 292, 2074, 2299
BlsI GCNGC 5 cut(s) 256, 290, 293, 2075, 2300
Bme1390I CCNGG 6 cut(s) 129, 642, 681, 1106, 2054, 2364
Bme18I GGWCC 5 cut(s) 336, 815, 1146, 2312, 2366
BmeRI GACNNNNNGTC 1 cut(s) 775
BmeT110I CYCGRG 1 cut(s) 1064
BmgBI CACGTC 1 cut(s) 1201
BmgT120I GGNCC 7 cut(s) 336, 605, 815, 1146, 1517, 2312, 2366
BmiI GGNNCC 2 cut(s) 808, 914
BmrFI CCNGG 6 cut(s) 129, 642, 681, 1106, 2054, 2364
BmrI ACTGGG 4 cut(s) 17, 155, 781, 1514
BmsI GCATC 2 cut(s) 1524, 1605
BmtI GCTAGC 2 cut(s) 1257, 2409
BmuI ACTGGG 4 cut(s) 17, 155, 781, 1514
BpiI GAAGAC 2 cut(s) 1291, 1391
BpmI CTGGAG 4 cut(s) 323, 616, 931, 1977
BpuEI CTTGAG 1 cut(s) 1567
BpuMI CCSGG 1 cut(s) 642
Bsa29I ATCGAT 1 cut(s) 1463
BsaAI YACGTR 1 cut(s) 1979
BsaBI GATNNNNATC 1 cut(s) 507
BsaHI GRCGYC 1 cut(s) 807
BsaJI CCNNGG 3 cut(s) 608, 916, 2004
BsaWI WCCGGW 1 cut(s) 1540
BsaXI ACNNNNNCTCC 4 cut(s) 904, 934, 2040, 2070
Bsc4I CCNNNNNNNGG 4 cut(s) 518, 1184, 2010, 2133
Bse1I ACTGG 7 cut(s) 12, 150, 599, 764, 776, 914, 1520
Bse3DI GCAATG 2 cut(s) 838, 1764
Bse8I GATNNNNATC 1 cut(s) 507
BseBI CCWGG 5 cut(s) 129, 681, 1106, 2054, 2364
BseCI ATCGAT 1 cut(s) 1463
BseDI CCNNGG 3 cut(s) 608, 916, 2004
BseGI GGATG 6 cut(s) 1539, 1662, 2201, 2284, 2317, 2359
BseJI GATNNNNATC 1 cut(s) 507
BseLI CCNNNNNNNGG 4 cut(s) 518, 1184, 2010, 2133
BseMI GCAATG 2 cut(s) 838, 1764
BseMII CTCAG 5 cut(s) 1079, 1230, 1920, 1973, 2364
BseNI ACTGG 7 cut(s) 12, 150, 599, 764, 776, 914, 1520
BseXI GCAGC 4 cut(s) 300, 303, 2085, 2310
Bsh1285I CGRYCG 1 cut(s) 1467
BshFI GGCC 5 cut(s) 132, 533, 607, 1518, 2329
BshNI GGYRCC 1 cut(s) 806
BshVI ATCGAT 1 cut(s) 1463
BsiEI CGRYCG 1 cut(s) 1467
BsiHKAI GWGCWC 1 cut(s) 309
BsiHKCI CYCGRG 1 cut(s) 1064
BsiSI CCGG 3 cut(s) 534, 641, 1541
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 4 cut(s) 518, 1184, 2010, 2133
BsmAI GTCTC 7 cut(s) 127, 255, 922, 944, 976, 1056, 1995
BsmBI CGTCTC 1 cut(s) 922
BsmFI GGGAC 1 cut(s) 123
BsnI GGCC 5 cut(s) 132, 533, 607, 1518, 2329
BsoBI CYCGRG 1 cut(s) 1064
Bsp1286I GDGCHC 1 cut(s) 309
Bsp1407I TGTACA 1 cut(s) 2242
Bsp143I GATC 9 cut(s) 508, 581, 613, 1192, 1464, 1623, 1695, 1741, 2104
Bsp19I CCATGG 1 cut(s) 2004
BspACI CCGC 4 cut(s) 254, 294, 339, 1204
BspANI GGCC 5 cut(s) 132, 533, 607, 1518, 2329
BspCNI CTCAG 5 cut(s) 1080, 1229, 1919, 1972, 2363
BspDI ATCGAT 1 cut(s) 1463
BspHI TCATGA 2 cut(s) 1030, 1825
BspLI GGNNCC 2 cut(s) 808, 914
BspMAI CTGCAG 1 cut(s) 367
BspOI GCTAGC 2 cut(s) 1257, 2409
BspPI GGATC 4 cut(s) 503, 589, 621, 1736
BspT107I GGYRCC 1 cut(s) 806
BsrDI GCAATG 2 cut(s) 838, 1764
BsrGI TGTACA 1 cut(s) 2242
BsrI ACTGG 7 cut(s) 12, 150, 599, 764, 776, 914, 1520
BssECI CCNNGG 3 cut(s) 608, 916, 2004
BssMI GATC 9 cut(s) 508, 581, 613, 1192, 1464, 1623, 1695, 1741, 2104
BssNI GRCGYC 1 cut(s) 807
BssT1I CCWWGG 2 cut(s) 608, 2004
Bst2UI CCWGG 5 cut(s) 129, 681, 1106, 2054, 2364
Bst4CI ACNGT 4 cut(s) 60, 665, 1083, 2430
Bst6I CTCTTC 2 cut(s) 1593, 2318
BstACI GRCGYC 1 cut(s) 807
BstAUI TGTACA 1 cut(s) 2242
BstBAI YACGTR 1 cut(s) 1979
BstC8I GCNNGC 4 cut(s) 1255, 1320, 1924, 2407
BstDEI CTNAG 7 cut(s) 1088, 1216, 1879, 1906, 1959, 2294, 2350
BstDSI CCRYGG 1 cut(s) 2004
BstF5I GGATG 6 cut(s) 1539, 1662, 2201, 2284, 2317, 2359
BstH2I RGCGCY 2 cut(s) 69, 810
BstHHI GCGC 2 cut(s) 68, 809
BstKTI GATC 9 cut(s) 511, 584, 616, 1195, 1467, 1626, 1698, 1744, 2107
BstMAI GTCTC 7 cut(s) 127, 255, 922, 944, 976, 1056, 1995
BstMBI GATC 9 cut(s) 508, 581, 613, 1192, 1464, 1623, 1695, 1741, 2104
BstMCI CGRYCG 1 cut(s) 1467
BstMWI GCNNNNNNNGC 2 cut(s) 1315, 2230
BstNI CCWGG 5 cut(s) 129, 681, 1106, 2054, 2364
BstNSI RCATGY 1 cut(s) 2228
BstSCI CCNGG 6 cut(s) 127, 640, 679, 1104, 2052, 2362
BstSFI CTRYAG 2 cut(s) 363, 2038
BstV1I GCAGC 4 cut(s) 300, 303, 2085, 2310
BstV2I GAAGAC 2 cut(s) 1291, 1391
BstX2I RGATCY 2 cut(s) 581, 1192
BstYI RGATCY 2 cut(s) 581, 1192
Bsu15I ATCGAT 1 cut(s) 1463
BsuI GTATCC 1 cut(s) 1720
BsuRI GGCC 5 cut(s) 132, 533, 607, 1518, 2329
BsuTUI ATCGAT 1 cut(s) 1463
BtgI CCRYGG 1 cut(s) 2004
BtrI CACGTC 1 cut(s) 1201
BtsCI GGATG 6 cut(s) 1539, 1662, 2201, 2284, 2317, 2359
BtsIMutI CAGTG 1 cut(s) 1088
Cac8I GCNNGC 4 cut(s) 1255, 1320, 1924, 2407
CaiI CAGNNNCTG 1 cut(s) 1087
CciI TCATGA 2 cut(s) 1030, 1825
CfoI GCGC 2 cut(s) 68, 809
Cfr13I GGNCC 7 cut(s) 336, 605, 815, 1146, 1517, 2312, 2366
ClaI ATCGAT 1 cut(s) 1463
CseI GACGC 1 cut(s) 1365
Csp6I GTAC 2 cut(s) 1019, 2243
CviQI GTAC 2 cut(s) 1019, 2243
DdeI CTNAG 7 cut(s) 1088, 1216, 1879, 1906, 1959, 2294, 2350
DinI GGCGCC 1 cut(s) 808
DpnI GATC 9 cut(s) 510, 583, 615, 1194, 1466, 1625, 1697, 1743, 2106
DpnII GATC 9 cut(s) 508, 581, 613, 1192, 1464, 1623, 1695, 1741, 2104
DraI TTTAAA 1 cut(s) 1860
DriI GACNNNNNGTC 1 cut(s) 775
Eam1104I CTCTTC 2 cut(s) 1593, 2318
Eam1105I GACNNNNNGTC 1 cut(s) 775
EarI CTCTTC 2 cut(s) 1593, 2318
EciI GGCGGA 2 cut(s) 328, 1193
Ecl136II GAGCTC 1 cut(s) 307
Eco130I CCWWGG 2 cut(s) 608, 2004
Eco147I AGGCCT 1 cut(s) 2329
Eco24I GRGCYC 1 cut(s) 309
Eco47I GGWCC 5 cut(s) 336, 815, 1146, 2312, 2366
Eco53kI GAGCTC 1 cut(s) 307
Eco57I CTGAAG 5 cut(s) 25, 90, 699, 1285, 1465
Eco72I CACGTG 1 cut(s) 1979
Eco88I CYCGRG 1 cut(s) 1064
EcoICRI GAGCTC 1 cut(s) 307
EcoRI GAATTC 1 cut(s) 1042
EcoRII CCWGG 5 cut(s) 127, 679, 1104, 2052, 2362
EcoT14I CCWWGG 2 cut(s) 608, 2004
EcoT22I ATGCAT 2 cut(s) 1117, 2134
EcoT38I GRGCYC 1 cut(s) 309
EgeI GGCGCC 1 cut(s) 808
EheI GGCGCC 1 cut(s) 808
ErhI CCWWGG 2 cut(s) 608, 2004
Esp3I CGTCTC 1 cut(s) 922
FalI AAGNNNNNCTT 2 cut(s) 592, 624
FaqI GGGAC 1 cut(s) 123
FauI CCCGC 1 cut(s) 287
FauNDI CATATG 1 cut(s) 1117
FbaI TGATCA 1 cut(s) 2104
FblI GTMKAC 1 cut(s) 2037
Fnu4HI GCNGC 5 cut(s) 255, 289, 292, 2074, 2299
FokI GGATG 6 cut(s) 1546, 1669, 2188, 2291, 2304, 2346
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 5 cut(s) 255, 289, 292, 2074, 2299
GlaI GCGC 2 cut(s) 67, 808
GluI GCNGC 5 cut(s) 255, 289, 292, 2074, 2299
GsuI CTGGAG 4 cut(s) 323, 616, 931, 1977
HaeII RGCGCY 2 cut(s) 69, 810
HaeIII GGCC 5 cut(s) 132, 533, 607, 1518, 2329
HapII CCGG 3 cut(s) 534, 641, 1541
HgaI GACGC 1 cut(s) 1365
HhaI GCGC 2 cut(s) 68, 809
Hin1I GRCGYC 1 cut(s) 807
Hin6I GCGC 2 cut(s) 66, 807
HinP1I GCGC 2 cut(s) 66, 807
HindIII AAGCTT 1 cut(s) 1720
HpaII CCGG 3 cut(s) 534, 641, 1541
HphI GGTGA 3 cut(s) 695, 1855, 2198
Hpy166II GTNNAC 4 cut(s) 815, 2038, 2243, 2315
Hpy8I GTNNAC 4 cut(s) 815, 2038, 2243, 2315
Hpy99I CGWCG 1 cut(s) 1359
HpyCH4III ACNGT 4 cut(s) 60, 665, 1083, 2430
HpyCH4IV ACGT 3 cut(s) 1200, 1338, 1978
HpyF10VI GCNNNNNNNGC 2 cut(s) 1315, 2230
HpyF3I CTNAG 7 cut(s) 1088, 1216, 1879, 1906, 1959, 2294, 2350
HpySE526I ACGT 3 cut(s) 1200, 1338, 1978
Hsp92I GRCGYC 1 cut(s) 807
HspAI GCGC 2 cut(s) 66, 807
KasI GGCGCC 1 cut(s) 806
Ksp22I TGATCA 1 cut(s) 2104
Kzo9I GATC 9 cut(s) 508, 581, 613, 1192, 1464, 1623, 1695, 1741, 2104
LmnI GCTCC 5 cut(s) 304, 410, 897, 912, 1432
Lsp1109I GCAGC 4 cut(s) 300, 303, 2085, 2310
LweI GCATC 2 cut(s) 1524, 1605
MaeII ACGT 3 cut(s) 1200, 1338, 1978
MaeIII GTNAC 6 cut(s) 16, 443, 634, 683, 1689, 1868
MalI GATC 9 cut(s) 510, 583, 615, 1194, 1466, 1625, 1697, 1743, 2106
MboI GATC 9 cut(s) 508, 581, 613, 1192, 1464, 1623, 1695, 1741, 2104
MfeI CAATTG 1 cut(s) 352
MflI RGATCY 2 cut(s) 581, 1192
MhlI GDGCHC 1 cut(s) 309
Mly113I GGCGCC 1 cut(s) 807
MlyI GAGTC 5 cut(s) 307, 440, 785, 836, 2058
MmeI TCCRAC 5 cut(s) 984, 1321, 1769, 1951, 2142
Mph1103I ATGCAT 2 cut(s) 1117, 2134
MroXI GAANNNNTTC 1 cut(s) 1194
MseI TTAA 8 cut(s) 576, 669, 1247, 1346, 1380, 1674, 1782, 1859
MslI CAYNNNNRTG 1 cut(s) 1392
MspA1I CMGCKG 1 cut(s) 294
MspI CCGG 3 cut(s) 534, 641, 1541
MspR9I CCNGG 6 cut(s) 129, 642, 681, 1106, 2054, 2364
MunI CAATTG 1 cut(s) 352
MvaI CCWGG 5 cut(s) 129, 681, 1106, 2054, 2364
MwoI GCNNNNNNNGC 2 cut(s) 1315, 2230
NarI GGCGCC 1 cut(s) 807
NciI CCSGG 1 cut(s) 642
NcoI CCATGG 1 cut(s) 2004
NdeI CATATG 1 cut(s) 1117
NdeII GATC 9 cut(s) 508, 581, 613, 1192, 1464, 1623, 1695, 1741, 2104
NheI GCTAGC 2 cut(s) 1253, 2405
NlaIV GGNNCC 2 cut(s) 808, 914
NmeAIII GCCGAG 1 cut(s) 941
NmuCI GTSAC 3 cut(s) 443, 634, 683
NsiI ATGCAT 2 cut(s) 1117, 2134
NspI RCATGY 1 cut(s) 2228
PaeR7I CTCGAG 1 cut(s) 1064
PagI TCATGA 2 cut(s) 1030, 1825
PceI AGGCCT 1 cut(s) 2329
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 5 cut(s) 965, 1384, 1829, 1894, 2189
PflMI CCANNNNNTGG 1 cut(s) 1184
PfoI TCCNGGA 1 cut(s) 2362
PkrI GCNGC 5 cut(s) 256, 290, 293, 2075, 2300
Ple19I CGATCG 1 cut(s) 1467
PleI GAGTC 5 cut(s) 306, 440, 784, 836, 2057
PluTI GGCGCC 1 cut(s) 810
PmaCI CACGTG 1 cut(s) 1979
PmlI CACGTG 1 cut(s) 1979
PpsI GAGTC 5 cut(s) 306, 440, 784, 836, 2057
Ppu21I YACGTR 1 cut(s) 1979
PshBI ATTAAT 1 cut(s) 576
PsiI TTATAA 2 cut(s) 1527, 2069
Psp124BI GAGCTC 1 cut(s) 309
Psp6I CCWGG 5 cut(s) 127, 679, 1104, 2052, 2362
PspCI CACGTG 1 cut(s) 1979
PspGI CCWGG 5 cut(s) 127, 679, 1104, 2052, 2362
PspN4I GGNNCC 2 cut(s) 808, 914
PspPI GGNCC 7 cut(s) 336, 605, 815, 1146, 1517, 2312, 2366
PspXI VCTCGAGB 1 cut(s) 1064
PstI CTGCAG 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 1087
PsuI RGATCY 2 cut(s) 581, 1192
PvuI CGATCG 1 cut(s) 1467
RsaI GTAC 2 cut(s) 1020, 2244
RsaNI GTAC 2 cut(s) 1019, 2243
RseI CAYNNNNRTG 1 cut(s) 1392
SacI GAGCTC 1 cut(s) 309
SaqAI TTAA 8 cut(s) 576, 669, 1247, 1346, 1380, 1674, 1782, 1859
SatI GCNGC 5 cut(s) 255, 289, 292, 2074, 2299
Sau3AI GATC 9 cut(s) 508, 581, 613, 1192, 1464, 1623, 1695, 1741, 2104
Sau96I GGNCC 7 cut(s) 336, 605, 815, 1146, 1517, 2312, 2366
SchI GAGTC 5 cut(s) 307, 440, 785, 836, 2058
ScrFI CCNGG 6 cut(s) 129, 642, 681, 1106, 2054, 2364
SduI GDGCHC 1 cut(s) 309
SfaNI GCATC 2 cut(s) 1524, 1605
SfcI CTRYAG 2 cut(s) 363, 2038
SfoI GGCGCC 1 cut(s) 808
Sfr274I CTCGAG 1 cut(s) 1064
SinI GGWCC 5 cut(s) 336, 815, 1146, 2312, 2366
SlaI CTCGAG 1 cut(s) 1064
SmiMI CAYNNNNRTG 1 cut(s) 1392
SmlI CTYRAG 2 cut(s) 1064, 1582
SmoI CTYRAG 2 cut(s) 1064, 1582
SpeI ACTAGT 1 cut(s) 1370
SseBI AGGCCT 1 cut(s) 2329
SsiI CCGC 4 cut(s) 254, 294, 339, 1204
SspDI GGCGCC 1 cut(s) 806
SspI AATATT 2 cut(s) 138, 1786
SstI GAGCTC 1 cut(s) 309
StuI AGGCCT 1 cut(s) 2329
StyD4I CCNGG 6 cut(s) 127, 640, 679, 1104, 2052, 2362
StyI CCWWGG 2 cut(s) 608, 2004
TaaI ACNGT 4 cut(s) 60, 665, 1083, 2430
TaiI ACGT 3 cut(s) 1203, 1341, 1981
TaqI TCGA 3 cut(s) 1065, 1402, 1463
TaqII GACCGA 1 cut(s) 1490
TatI WGTACW 1 cut(s) 2242
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 5 cut(s) 965, 1384, 1829, 1894, 2189
Tru1I TTAA 8 cut(s) 576, 669, 1247, 1346, 1380, 1674, 1782, 1859
Tru9I TTAA 8 cut(s) 576, 669, 1247, 1346, 1380, 1674, 1782, 1859
TscAI CASTG 1 cut(s) 1088
TseFI GTSAC 3 cut(s) 443, 634, 683
TseI GCWGC 4 cut(s) 288, 291, 2073, 2298
Tsp45I GTSAC 3 cut(s) 443, 634, 683
TspGWI ACGGA 2 cut(s) 2273, 2291
TspRI CASTG 1 cut(s) 1088
Van91I CCANNNNNTGG 1 cut(s) 1184
VpaK11BI GGWCC 5 cut(s) 336, 815, 1146, 2312, 2366
VspI ATTAAT 1 cut(s) 576
XapI RAATTY 4 cut(s) 675, 937, 1042, 2386
XbaI TCTAGA 1 cut(s) 1054
XceI RCATGY 1 cut(s) 2228
XcmI CCANNNNNNNNNTGG 2 cut(s) 181, 1135
XhoI CTCGAG 1 cut(s) 1064
XmiI GTMKAC 1 cut(s) 2037
XmnI GAANNNNTTC 1 cut(s) 1194
Zsp2I ATGCAT 2 cut(s) 1117, 2134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.