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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1063 |
| Acc36I | ACCTGC | 2 cut(s) | 1063, 1149 |
| AccB1I | GGYRCC | 1 cut(s) | 4 |
| AciI | CCGC | 5 cut(s) | 321, 1131, 1371, 1937, 1940 |
| AclWI | GGATC | 6 cut(s) | 503, 584, 1400, 1535, 1577, 1590 |
| AcoI | YGGCCR | 1 cut(s) | 1380 |
| AcsI | RAATTY | 3 cut(s) | 367, 770, 1149 |
| AcyI | GRCGYC | 1 cut(s) | 5 |
| AdeI | CACNNNGTG | 1 cut(s) | 608 |
| AfaI | GTAC | 6 cut(s) | 247, 727, 852, 1096, 1596, 1972 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 155, 469, 806, 1848 |
| AgsI | TTSAA | 5 cut(s) | 775, 984, 1046, 1155, 1985 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 657 |
| AhlI | ACTAGT | 1 cut(s) | 656 |
| AjnI | CCWGG | 4 cut(s) | 805, 955, 1346, 1570 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1256, 1288 |
| Alw21I | GWGCWC | 3 cut(s) | 530, 1446, 1819 |
| Alw26I | GTCTC | 7 cut(s) | 552, 644, 729, 815, 1210, 1443, 1541 |
| Alw44I | GTGCAC | 1 cut(s) | 1442 |
| AlwI | GGATC | 6 cut(s) | 503, 584, 1400, 1535, 1577, 1590 |
| AoxI | GGCC | 4 cut(s) | 207, 388, 1380, 1838 |
| ApaLI | GTGCAC | 1 cut(s) | 1442 |
| ApeKI | GCWGC | 1 cut(s) | 318 |
| ApoI | RAATTY | 3 cut(s) | 367, 770, 1149 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1271 |
| AspLEI | GCGC | 1 cut(s) | 7 |
| AspS9I | GGNCC | 5 cut(s) | 207, 472, 803, 1711, 1839 |
| AsuC2I | CCSGG | 1 cut(s) | 801 |
| AsuHPI | GGTGA | 3 cut(s) | 571, 1310, 1811 |
| AsuII | TTCGAA | 1 cut(s) | 1828 |
| AvaII | GGWCC | 3 cut(s) | 472, 803, 1711 |
| BaeGI | GKGCMC | 1 cut(s) | 1446 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1119, 1152 |
| BalI | TGGCCA | 1 cut(s) | 1382 |
| BamHI | GGATCC | 1 cut(s) | 1582 |
| BanI | GGYRCC | 1 cut(s) | 4 |
| BbsI | GAAGAC | 1 cut(s) | 1274 |
| Bbv12I | GWGCWC | 3 cut(s) | 530, 1446, 1819 |
| BbvI | GCAGC | 1 cut(s) | 330 |
| BccI | CCATC | 4 cut(s) | 548, 883, 1239, 1982 |
| BceAI | ACGGC | 1 cut(s) | 180 |
| BciT130I | CCWGG | 4 cut(s) | 807, 957, 1348, 1572 |
| BciVI | GTATCC | 1 cut(s) | 807 |
| BcnI | CCSGG | 1 cut(s) | 801 |
| BcoDI | GTCTC | 7 cut(s) | 552, 644, 729, 815, 1210, 1443, 1541 |
| BcuI | ACTAGT | 1 cut(s) | 656 |
| BfaI | CTAG | 7 cut(s) | 386, 657, 1092, 1482, 1499, 1538, 1611 |
| BfmI | CTRYAG | 3 cut(s) | 153, 583, 642 |
| BfoI | RGCGCY | 1 cut(s) | 8 |
| BfuAI | ACCTGC | 2 cut(s) | 1063, 1149 |
| BfuI | GTATCC | 1 cut(s) | 807 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 201 |
| BglII | AGATCT | 1 cut(s) | 831 |
| BisI | GCNGC | 2 cut(s) | 319, 1940 |
| BlsI | GCNGC | 2 cut(s) | 320, 1941 |
| Bme1390I | CCNGG | 5 cut(s) | 801, 807, 957, 1348, 1572 |
| Bme18I | GGWCC | 3 cut(s) | 472, 803, 1711 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 657 |
| BmgT120I | GGNCC | 5 cut(s) | 207, 472, 803, 1711, 1839 |
| BmiI | GGNNCC | 7 cut(s) | 6, 473, 474, 1012, 1028, 1584, 1712 |
| BmrFI | CCNGG | 5 cut(s) | 801, 807, 957, 1348, 1572 |
| BmrI | ACTGGG | 1 cut(s) | 1563 |
| BmsI | GCATC | 6 cut(s) | 97, 130, 1303, 1785, 1867, 1896 |
| BmuI | ACTGGG | 1 cut(s) | 1563 |
| BpiI | GAAGAC | 1 cut(s) | 1274 |
| BpmI | CTGGAG | 1 cut(s) | 1686 |
| Bpu14I | TTCGAA | 1 cut(s) | 1828 |
| BpuEI | CTTGAG | 1 cut(s) | 1234 |
| BpuMI | CCSGG | 1 cut(s) | 801 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1410 |
| BsaHI | GRCGYC | 1 cut(s) | 5 |
| BsaI | GGTCTC | 1 cut(s) | 815 |
| BsaJI | CCNNGG | 3 cut(s) | 533, 911, 1347 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1256, 1286 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 155, 469, 806, 1848 |
| Bse1I | ACTGG | 5 cut(s) | 733, 854, 1558, 1669, 1698 |
| Bse3DI | GCAATG | 2 cut(s) | 1388, 1439 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1410 |
| BseBI | CCWGG | 4 cut(s) | 807, 957, 1348, 1572 |
| BseDI | CCNNGG | 3 cut(s) | 533, 911, 1347 |
| BseGI | GGATG | 6 cut(s) | 288, 894, 1250, 1357, 1467, 1630 |
| BseJI | GATNNNNATC | 1 cut(s) | 1410 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 155, 469, 806, 1848 |
| BseMI | GCAATG | 2 cut(s) | 1388, 1439 |
| BseMII | CTCAG | 6 cut(s) | 210, 619, 687, 1059, 1612, 1959 |
| BseNI | ACTGG | 5 cut(s) | 733, 854, 1558, 1669, 1698 |
| BseRI | GAGGAG | 4 cut(s) | 662, 1023, 1277, 1778 |
| BseSI | GKGCMC | 1 cut(s) | 1446 |
| BseXI | GCAGC | 1 cut(s) | 330 |
| BseYI | CCCAGC | 2 cut(s) | 740, 884 |
| BshFI | GGCC | 4 cut(s) | 209, 390, 1382, 1840 |
| BshNI | GGYRCC | 1 cut(s) | 4 |
| BsiHKAI | GWGCWC | 3 cut(s) | 530, 1446, 1819 |
| BsiSI | CCGG | 3 cut(s) | 420, 801, 1014 |
| BsiWI | CGTACG | 1 cut(s) | 245 |
| BslFI | GGGAC | 4 cut(s) | 485, 757, 1704, 1724 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 155, 469, 806, 1848 |
| BsmAI | GTCTC | 7 cut(s) | 552, 644, 729, 815, 1210, 1443, 1541 |
| BsmBI | CGTCTC | 1 cut(s) | 729 |
| BsmFI | GGGAC | 4 cut(s) | 485, 757, 1704, 1724 |
| BsmI | GAATGC | 1 cut(s) | 329 |
| BsnI | GGCC | 4 cut(s) | 209, 390, 1382, 1840 |
| Bso31I | GGTCTC | 1 cut(s) | 815 |
| Bsp119I | TTCGAA | 1 cut(s) | 1828 |
| Bsp1286I | GDGCHC | 3 cut(s) | 530, 1446, 1819 |
| Bsp143I | GATC | 7 cut(s) | 495, 576, 676, 831, 1405, 1540, 1582 |
| BspACI | CCGC | 5 cut(s) | 321, 1131, 1371, 1937, 1940 |
| BspANI | GGCC | 4 cut(s) | 209, 390, 1382, 1840 |
| BspCNI | CTCAG | 6 cut(s) | 211, 618, 688, 1060, 1611, 1958 |
| BspHI | TCATGA | 2 cut(s) | 15, 1402 |
| BspLI | GGNNCC | 7 cut(s) | 6, 473, 474, 1012, 1028, 1584, 1712 |
| BspMAI | CTGCAG | 1 cut(s) | 646 |
| BspMI | ACCTGC | 2 cut(s) | 1063, 1149 |
| BspPI | GGATC | 6 cut(s) | 503, 584, 1400, 1535, 1577, 1590 |
| BspT104I | TTCGAA | 1 cut(s) | 1828 |
| BspT107I | GGYRCC | 1 cut(s) | 4 |
| BspTNI | GGTCTC | 1 cut(s) | 815 |
| BsrDI | GCAATG | 2 cut(s) | 1388, 1439 |
| BsrI | ACTGG | 5 cut(s) | 733, 854, 1558, 1669, 1698 |
| BssECI | CCNNGG | 3 cut(s) | 533, 911, 1347 |
| BssMI | GATC | 7 cut(s) | 495, 576, 676, 831, 1405, 1540, 1582 |
| BssNI | GRCGYC | 1 cut(s) | 5 |
| BssT1I | CCWWGG | 1 cut(s) | 533 |
| Bst2UI | CCWGG | 4 cut(s) | 807, 957, 1348, 1572 |
| Bst4CI | ACNGT | 6 cut(s) | 410, 587, 709, 998, 1099, 1809 |
| BstACI | GRCGYC | 1 cut(s) | 5 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 149 |
| BstBI | TTCGAA | 1 cut(s) | 1828 |
| BstC8I | GCNNGC | 3 cut(s) | 76, 150, 526 |
| BstDEI | CTNAG | 7 cut(s) | 219, 605, 696, 1068, 1598, 1899, 1945 |
| BstEII | GGTNACC | 1 cut(s) | 1109 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 153, 1846 |
| BstF5I | GGATG | 6 cut(s) | 288, 894, 1250, 1357, 1467, 1630 |
| BstH2I | RGCGCY | 1 cut(s) | 8 |
| BstHHI | GCGC | 1 cut(s) | 7 |
| BstKTI | GATC | 7 cut(s) | 498, 579, 679, 834, 1408, 1543, 1585 |
| BstMAI | GTCTC | 7 cut(s) | 552, 644, 729, 815, 1210, 1443, 1541 |
| BstMBI | GATC | 7 cut(s) | 495, 576, 676, 831, 1405, 1540, 1582 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 149, 201, 215 |
| BstNI | CCWGG | 4 cut(s) | 807, 957, 1348, 1572 |
| BstNSI | RCATGY | 1 cut(s) | 491 |
| BstPI | GGTNACC | 1 cut(s) | 1109 |
| BstSCI | CCNGG | 5 cut(s) | 799, 805, 955, 1346, 1570 |
| BstSFI | CTRYAG | 3 cut(s) | 153, 583, 642 |
| BstSLI | GKGCMC | 1 cut(s) | 1446 |
| BstV1I | GCAGC | 1 cut(s) | 330 |
| BstV2I | GAAGAC | 1 cut(s) | 1274 |
| BstX2I | RGATCY | 3 cut(s) | 831, 1540, 1582 |
| BstYI | RGATCY | 3 cut(s) | 831, 1540, 1582 |
| BsuI | GTATCC | 1 cut(s) | 807 |
| BsuRI | GGCC | 4 cut(s) | 209, 390, 1382, 1840 |
| BtsCI | GGATG | 6 cut(s) | 288, 894, 1250, 1357, 1467, 1630 |
| BtsI | GCAGTG | 2 cut(s) | 143, 1383 |
| BtsIMutI | CAGTG | 7 cut(s) | 143, 258, 415, 592, 613, 861, 1383 |
| BveI | ACCTGC | 2 cut(s) | 1063, 1149 |
| Cac8I | GCNNGC | 3 cut(s) | 76, 150, 526 |
| CciI | TCATGA | 2 cut(s) | 15, 1402 |
| CfoI | GCGC | 1 cut(s) | 7 |
| Cfr13I | GGNCC | 5 cut(s) | 207, 472, 803, 1711, 1839 |
| CseI | GACGC | 1 cut(s) | 746 |
| CsiI | ACCWGGT | 1 cut(s) | 1570 |
| Csp6I | GTAC | 6 cut(s) | 246, 726, 851, 1095, 1595, 1971 |
| CviAII | CATG | 4 cut(s) | 16, 488, 1294, 1403 |
| CviQI | GTAC | 6 cut(s) | 246, 726, 851, 1095, 1595, 1971 |
| DdeI | CTNAG | 7 cut(s) | 219, 605, 696, 1068, 1598, 1899, 1945 |
| DinI | GGCGCC | 1 cut(s) | 6 |
| DpnI | GATC | 7 cut(s) | 497, 578, 678, 833, 1407, 1542, 1584 |
| DpnII | GATC | 7 cut(s) | 495, 576, 676, 831, 1405, 1540, 1582 |
| DraIII | CACNNNGTG | 1 cut(s) | 608 |
| DriI | GACNNNNNGTC | 1 cut(s) | 657 |
| EaeI | YGGCCR | 1 cut(s) | 1380 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 657 |
| Eco130I | CCWWGG | 1 cut(s) | 533 |
| Eco147I | AGGCCT | 1 cut(s) | 390 |
| Eco31I | GGTCTC | 1 cut(s) | 815 |
| Eco32I | GATATC | 1 cut(s) | 353 |
| Eco47I | GGWCC | 3 cut(s) | 472, 803, 1711 |
| Eco91I | GGTNACC | 1 cut(s) | 1109 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 153, 1846 |
| EcoO109I | RGGNCCY | 3 cut(s) | 472, 1711, 1839 |
| EcoO65I | GGTNACC | 1 cut(s) | 1109 |
| EcoRI | GAATTC | 1 cut(s) | 770 |
| EcoRII | CCWGG | 4 cut(s) | 805, 955, 1346, 1570 |
| EcoRV | GATATC | 1 cut(s) | 353 |
| EcoT14I | CCWWGG | 1 cut(s) | 533 |
| EgeI | GGCGCC | 1 cut(s) | 6 |
| EheI | GGCGCC | 1 cut(s) | 6 |
| ErhI | CCWWGG | 1 cut(s) | 533 |
| Esp3I | CGTCTC | 1 cut(s) | 729 |
| FaeI | CATG | 4 cut(s) | 19, 491, 1297, 1406 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 818, 850, 964, 996, 1015, 1047, 1716, 1748 |
| FaqI | GGGAC | 4 cut(s) | 485, 757, 1704, 1724 |
| FatI | CATG | 4 cut(s) | 15, 487, 1293, 1402 |
| FauI | CCCGC | 1 cut(s) | 1364 |
| Fnu4HI | GCNGC | 2 cut(s) | 319, 1940 |
| FokI | GGATG | 6 cut(s) | 275, 901, 1257, 1364, 1454, 1637 |
| Fsp4HI | GCNGC | 2 cut(s) | 319, 1940 |
| FspBI | CTAG | 7 cut(s) | 386, 657, 1092, 1482, 1499, 1538, 1611 |
| GlaI | GCGC | 1 cut(s) | 6 |
| GluI | GCNGC | 2 cut(s) | 319, 1940 |
| GsaI | CCCAGC | 2 cut(s) | 744, 888 |
| GsuI | CTGGAG | 1 cut(s) | 1686 |
| HaeII | RGCGCY | 1 cut(s) | 8 |
| HaeIII | GGCC | 4 cut(s) | 209, 390, 1382, 1840 |
| HapII | CCGG | 3 cut(s) | 420, 801, 1014 |
| HgaI | GACGC | 1 cut(s) | 746 |
| HhaI | GCGC | 1 cut(s) | 7 |
| Hin1I | GRCGYC | 1 cut(s) | 5 |
| Hin1II | CATG | 4 cut(s) | 19, 491, 1297, 1406 |
| Hin6I | GCGC | 1 cut(s) | 5 |
| HinP1I | GCGC | 1 cut(s) | 5 |
| HindIII | AAGCTT | 2 cut(s) | 1730, 1962 |
| HinfI | GANTC | 5 cut(s) | 225, 942, 1478, 1619, 1682 |
| HpaII | CCGG | 3 cut(s) | 420, 801, 1014 |
| HphI | GGTGA | 3 cut(s) | 571, 1310, 1811 |
| Hpy166II | GTNNAC | 6 cut(s) | 460, 904, 1102, 1285, 1444, 1681 |
| Hpy188I | TCNGA | 7 cut(s) | 756, 815, 879, 912, 1039, 1069, 1624 |
| Hpy188III | TCNNGA | 9 cut(s) | 16, 235, 457, 946, 992, 1403, 1544, 1586, 1985 |
| Hpy8I | GTNNAC | 6 cut(s) | 460, 904, 1102, 1285, 1444, 1681 |
| HpyAV | CCTTC | 7 cut(s) | 247, 463, 1017, 1076, 1124, 1324, 1991 |
| HpyCH4III | ACNGT | 6 cut(s) | 410, 587, 709, 998, 1099, 1809 |
| HpyCH4IV | ACGT | 1 cut(s) | 1271 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 149, 201, 215 |
| HpyF3I | CTNAG | 7 cut(s) | 219, 605, 696, 1068, 1598, 1899, 1945 |
| HpySE526I | ACGT | 1 cut(s) | 1271 |
| Hsp92I | GRCGYC | 1 cut(s) | 5 |
| Hsp92II | CATG | 4 cut(s) | 19, 491, 1297, 1406 |
| HspAI | GCGC | 1 cut(s) | 5 |
| KasI | GGCGCC | 1 cut(s) | 4 |
| KflI | GGGWCCC | 1 cut(s) | 472 |
| Kzo9I | GATC | 7 cut(s) | 495, 576, 676, 831, 1405, 1540, 1582 |
| LmnI | GCTCC | 5 cut(s) | 346, 618, 959, 1010, 1958 |
| Lsp1109I | GCAGC | 1 cut(s) | 330 |
| LweI | GCATC | 6 cut(s) | 97, 130, 1303, 1785, 1867, 1896 |
| MabI | ACCWGGT | 1 cut(s) | 1570 |
| MaeI | CTAG | 7 cut(s) | 386, 657, 1092, 1482, 1499, 1538, 1611 |
| MaeII | ACGT | 1 cut(s) | 1271 |
| MaeIII | GTNAC | 6 cut(s) | 256, 410, 703, 946, 1001, 1109 |
| MalI | GATC | 7 cut(s) | 497, 578, 678, 833, 1407, 1542, 1584 |
| MboI | GATC | 7 cut(s) | 495, 576, 676, 831, 1405, 1540, 1582 |
| MfeI | CAATTG | 2 cut(s) | 613, 1857 |
| MflI | RGATCY | 3 cut(s) | 831, 1540, 1582 |
| MhlI | GDGCHC | 3 cut(s) | 530, 1446, 1819 |
| MlsI | TGGCCA | 1 cut(s) | 1382 |
| MluNI | TGGCCA | 1 cut(s) | 1382 |
| Mly113I | GGCGCC | 1 cut(s) | 5 |
| MlyI | GAGTC | 3 cut(s) | 1472, 1628, 1676 |
| MmeI | TCCRAC | 1 cut(s) | 1602 |
| Mox20I | TGGCCA | 1 cut(s) | 1382 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1271 |
| MscI | TGGCCA | 1 cut(s) | 1382 |
| MseI | TTAA | 5 cut(s) | 836, 1343, 1455, 1488, 1967 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1875 |
| Msp20I | TGGCCA | 1 cut(s) | 1382 |
| MspA1I | CMGCKG | 5 cut(s) | 321, 374, 722, 1133, 1742 |
| MspI | CCGG | 3 cut(s) | 420, 801, 1014 |
| MspR9I | CCNGG | 5 cut(s) | 801, 807, 957, 1348, 1572 |
| MunI | CAATTG | 2 cut(s) | 613, 1857 |
| Mva1269I | GAATGC | 1 cut(s) | 329 |
| MvaI | CCWGG | 4 cut(s) | 807, 957, 1348, 1572 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 149, 201, 215 |
| NarI | GGCGCC | 1 cut(s) | 5 |
| NciI | CCSGG | 1 cut(s) | 801 |
| NdeII | GATC | 7 cut(s) | 495, 576, 676, 831, 1405, 1540, 1582 |
| NlaIII | CATG | 4 cut(s) | 19, 491, 1297, 1406 |
| NlaIV | GGNNCC | 7 cut(s) | 6, 473, 474, 1012, 1028, 1584, 1712 |
| NmeAIII | GCCGAG | 1 cut(s) | 1498 |
| NmuCI | GTSAC | 5 cut(s) | 256, 410, 703, 946, 1001 |
| NspI | RCATGY | 1 cut(s) | 491 |
| NspV | TTCGAA | 1 cut(s) | 1828 |
| PagI | TCATGA | 2 cut(s) | 15, 1402 |
| PaqCI | CACCTGC | 1 cut(s) | 1063 |
| PceI | AGGCCT | 1 cut(s) | 390 |
| PctI | GAATGC | 1 cut(s) | 329 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1271 |
| PfeI | GAWTC | 2 cut(s) | 225, 942 |
| Pfl23II | CGTACG | 1 cut(s) | 245 |
| PkrI | GCNGC | 2 cut(s) | 320, 1941 |
| PleI | GAGTC | 3 cut(s) | 1472, 1627, 1676 |
| PluTI | GGCGCC | 1 cut(s) | 8 |
| PpsI | GAGTC | 3 cut(s) | 1472, 1627, 1676 |
| PpuMI | RGGWCCY | 2 cut(s) | 472, 1711 |
| Psp5II | RGGWCCY | 2 cut(s) | 472, 1711 |
| Psp6I | CCWGG | 4 cut(s) | 805, 955, 1346, 1570 |
| PspEI | GGTNACC | 1 cut(s) | 1109 |
| PspFI | CCCAGC | 2 cut(s) | 740, 884 |
| PspGI | CCWGG | 4 cut(s) | 805, 955, 1346, 1570 |
| PspLI | CGTACG | 1 cut(s) | 245 |
| PspN4I | GGNNCC | 7 cut(s) | 6, 473, 474, 1012, 1028, 1584, 1712 |
| PspPI | GGNCC | 5 cut(s) | 207, 472, 803, 1711, 1839 |
| PspPPI | RGGWCCY | 2 cut(s) | 472, 1711 |
| PstI | CTGCAG | 1 cut(s) | 646 |
| PsuI | RGATCY | 3 cut(s) | 831, 1540, 1582 |
| PvuII | CAGCTG | 3 cut(s) | 374, 722, 1742 |
| RsaI | GTAC | 6 cut(s) | 247, 727, 852, 1096, 1596, 1972 |
| RsaNI | GTAC | 6 cut(s) | 246, 726, 851, 1095, 1595, 1971 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1875 |
| SaqAI | TTAA | 5 cut(s) | 836, 1343, 1455, 1488, 1967 |
| SatI | GCNGC | 2 cut(s) | 319, 1940 |
| Sau3AI | GATC | 7 cut(s) | 495, 576, 676, 831, 1405, 1540, 1582 |
| Sau96I | GGNCC | 5 cut(s) | 207, 472, 803, 1711, 1839 |
| SchI | GAGTC | 3 cut(s) | 1472, 1628, 1676 |
| ScrFI | CCNGG | 5 cut(s) | 801, 807, 957, 1348, 1572 |
| SduI | GDGCHC | 3 cut(s) | 530, 1446, 1819 |
| SexAI | ACCWGGT | 1 cut(s) | 1570 |
| SfaNI | GCATC | 6 cut(s) | 97, 130, 1303, 1785, 1867, 1896 |
| SfcI | CTRYAG | 3 cut(s) | 153, 583, 642 |
| SfoI | GGCGCC | 1 cut(s) | 6 |
| SfuI | TTCGAA | 1 cut(s) | 1828 |
| SinI | GGWCC | 3 cut(s) | 472, 803, 1711 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1875 |
| SmlI | CTYRAG | 1 cut(s) | 1213 |
| SmoI | CTYRAG | 1 cut(s) | 1213 |
| SpeI | ACTAGT | 1 cut(s) | 656 |
| SseBI | AGGCCT | 1 cut(s) | 390 |
| SsiI | CCGC | 5 cut(s) | 321, 1131, 1371, 1937, 1940 |
| SspDI | GGCGCC | 1 cut(s) | 4 |
| SspI | AATATT | 1 cut(s) | 541 |
| SspMI | CTAG | 7 cut(s) | 386, 657, 1092, 1482, 1499, 1538, 1611 |
| StuI | AGGCCT | 1 cut(s) | 390 |
| StyD4I | CCNGG | 5 cut(s) | 799, 805, 955, 1346, 1570 |
| StyI | CCWWGG | 1 cut(s) | 533 |
| TaaI | ACNGT | 6 cut(s) | 410, 587, 709, 998, 1099, 1809 |
| TaiI | ACGT | 1 cut(s) | 1274 |
| TaqI | TCGA | 4 cut(s) | 165, 779, 1828, 1915 |
| TatI | WGTACW | 2 cut(s) | 1594, 1970 |
| TauI | GCSGC | 1 cut(s) | 1942 |
| TfiI | GAWTC | 2 cut(s) | 225, 942 |
| Tru1I | TTAA | 5 cut(s) | 836, 1343, 1455, 1488, 1967 |
| Tru9I | TTAA | 5 cut(s) | 836, 1343, 1455, 1488, 1967 |
| TscAI | CASTG | 7 cut(s) | 150, 265, 415, 592, 613, 861, 1390 |
| TseFI | GTSAC | 5 cut(s) | 256, 410, 703, 946, 1001 |
| TseI | GCWGC | 1 cut(s) | 318 |
| Tsp45I | GTSAC | 5 cut(s) | 256, 410, 703, 946, 1001 |
| TspDTI | ATGAA | 8 cut(s) | 32, 654, 854, 855, 1282, 1391, 1406, 1848 |
| TspGWI | ACGGA | 1 cut(s) | 1205 |
| TspRI | CASTG | 7 cut(s) | 150, 265, 415, 592, 613, 861, 1390 |
| VneI | GTGCAC | 1 cut(s) | 1442 |
| VpaK11BI | GGWCC | 3 cut(s) | 472, 803, 1711 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 153, 1846 |
| XapI | RAATTY | 3 cut(s) | 367, 770, 1149 |
| XceI | RCATGY | 1 cut(s) | 491 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1271 |
| XspI | CTAG | 7 cut(s) | 386, 657, 1092, 1482, 1499, 1538, 1611 |