Rorug07G0111600

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000187
Physical Location & Seq
Reverse (-)
8712986 .. 8718531
5546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0111600.1

Sequence Viewer

Length: 3147 bp
ATGGAAAGTCATTGTCTCAATCCTATTAGCTTGGTCCTTTCAGAAGACAAACTGATTATATCATCAACAAGTCATGGTTGTGTTTATCTTACTTTATCTTCCACCATTTTGAGGGTAGCAATAATCAACCGAGCACCGGAAGAGATGGTTCATGATTTCGAAGCTTGTGCAGATTGCACTCAGAAGTGTTTGCTGATTCATGGGAATAAGAAAAGTGGTTCCCATATCGAAACCTCTTTTTTCAAAATCATGTTTGGAAAACAGTTTTCTAAATTTATGGATTTGCCTCCAAAAGTTGCTCGTATTTTGTTATTGTCTGATCGGAGAACTTTTCTTGAAGATTCATGTGGACTGCGGTGGAAAGTTGCAATATCTAGTGTTAATGGTTCCTGGGCCTTTCAACAAGGATGGAATGCTTTTGCACTAGACCATGACCTACAAGTAGGAGATTTTCTGGTCTTCAATTACGTTGTGGGTTCACATTTTACTGTTAAGATATATGACAACTCTGGGTGTGAAGTGAATCTTTTTGAGACCATACATCAGAACAACAGAAATAGGGTTCACACAGATGGTCAGTGCCACACCATTGGTAAAAGTACAGTGAGCAAATCCGTTTCAGATACCTTTGCTGGTCCAGATGCAGAAATAAGTAAATGCTTGAATGAAGTGCATGGTATGAAGAAACCTCTAATTATCACAGAAAATGCTTCAGATCACGATGATGATTATGAAAGGTCAAAGTGCAAGCGCAAAGCAGAATTTGCTGACGACATGTCATGTATGATAGAAAGAGAATATGGACACCAACAAGGAGATAACAGAGCCCGTATTCTTGATTTGTCTAGTTTTGAATCATTCAAAAAGATAAAAATCTGTGGCAGTGATGGAAGCAATAAAGTTGCTGCAGTGAATGAAACACATTCTCATCATGCTGATTCATCACTGAAATTGAGAAATGAAGTTAGCTCAGTTAAAATTCCAGTGGCTAAAGAGTTAGTGGATAGAGTAATTCCTTCAGATGCATCTGAAATTGAAAGGACTCAGAAAAATAACTATTCTGAATACAAGGTAAAGGGGGCACCCCTGTCTGATAAAGATTCATCCACCAATGTGACCTCTGGACATCTCTTTCCAACACTTCCTGAAAATCCTGAACAAAAGGAAGAGATCTTCTCTAGTATTTCGAACAGAGCAGCCAAAAAATGCCAGTCTGCTAATGTATCAGGCAATGATCATCAGGTAGCAGTATCAGGAAATGAACATGATTTGATTGATGACACTTGTCAAAGTGCCAACAAAAAAATTACTGAAATTTCAAAGTCCTGCGATAAAGTGATGAAGGTCATTGAAAACAAGCCTGTGGAGTTGACTTCACATCCATGCGGTCAGGAGGTCATTCCAGAAAGTAATGATATTGTGATGATGAACATTGGAAAAGAGTCTGTGGATTTGACTTCAGATCCATGCAGTCGGGAGGTCATTCCAGAAAGAAAGGATAAAGCTGGGACCATCATTGAAAATGTTATTGTGGACTTGACTTCAGATCAATGCAGTCAGGAGGTCATTGAAAAAGAGGAATCATTAGATCTTTCCACCAAGGTTGGAATAGAAATTCCTTCAGGTCCAGCTGAGTTTGAAGGGACTGAGGAATGTAACAAATCTGAAGCCAAGGTCAATAGGGTGCCGTCTGATAAAGATTCATGCACTGAGACCTCTGGGCATCCCTTCCCAACACATTCTGAAAATCCTGAAGAAAATGGAGAGACCACCTCAAAGAAAGCAACAAAAGAATGCCGGAATGCTGATGGATCAGAATTATTATCAGGCAAGGATCAGGTGGTACTATCACAAAAGGAACCTGATTTAATGTATGCTAGTTCCCCAAGAGACAACAAGAAATTGACTGGGATTTCAGAGTCTATTCATAATCCCCTTACTTTTGAATGCCAAAATTATGATGTGATCATCAATGAGAAGGAGTCTGTTGAGGTGACTTGTCAGGAGGTCATTCCACAGAGAAAGGATACCATCATCATTGAAAACAAGTCTGTTGAGGTGACTTTAGAACCATGCAGCCAGGAGGTTATTCCAGAAAGAAAGGATACAATCGTCGTTGGAGAAGAGTCTGTTGATTTGACTTTAGATCCAAGCTGTCAAGAGTTCATTCCAGAAACAAAGGGGGAATCATTAAAGGTTTTCAACAGGGTACACAGACAGCGTGCTAATACAAATATAAGAGCAGTTCCTTCAGATGCATCTGACCTTGAAAGGACTGAGAAAAGAAATTATTCTGAAGTTAAGGTCAGAAAGGTATCAACTTCTGATAAAGTTCCACACACCAAGCAGACCTCTTGTCATCTCAACCCAATACGTCATGTACATGTTGAAGAAGATAAAACAAACATCCCTAAGATTTCAAACAGAGGAATAAACAAACTTAAGACTACTGATGGATCAGTGGCTGCATCCCAAAAGGAACAAGTTTCTATCGATGACAGCTGCCAACAAGCCAAAAAGAAATTTACTGGAATTTCAAAGTCTTTTGAAGCTCCGGTTAATTCTGGATCCCAATATTATTCAGCTAAAATGTCAAAGATCATGGAAAAGGAGTTTGTAAACTCCAGTTCAGATCCATGCAGTCAGGAGATTATTCCAGAAGTTGATGCCCTATCATCAAAGATCTCTAACAAGTTGCAGAGTCAGAGTAATTTTGCAAGAAAAAATGACAGCGCTCTTCCAGTGGTAAAACCTGAACCCATTGAAGATGCAGGTGCAACCTTTCCAAGTTTCACAATCTCCTGTTTGGCAACTGACCGTCAATCTTTTCTGGAGTTGCCAAAGAGTTTGCCATTACCCTTGAACACCATGAAGGGAAGGTCTAAACTGGATAGGAGGATAGTTTATCTTCAAGGTCCTGATAAGAGATTGTGGCCAGTCCTCTACATTCAGAAATTTTTCTTCAGAACTTTGGCAAATGGTTGGGAGGCTTTCAGCATGGCAAATAACATTGAACAAGGAGATCGATGTACTATTGAGGTCACAAATCAAACTGAAGGCATATGTAGTGTGCACATAGTTAAGATGAGAGATGATATGGAATTCTATGAAGAGTATGAGCACAATCCTCTCATTGACCTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

1048

Amino Acids

116.92

Weight (kDa)

5.48

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 80 - 169 4.9e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000187)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20170 FvH4_4g06831
rosa_chinensis RchiOBHm_Chr1g0321991 RchiOBHm_Chr2g0161811
rosa_laevigata RLG00000015375 RLG00000018377
rosa_multiflora Rmu_co8011754.1_g000001 Rmu_co8019986.1_g000001 Rmu_co8067766.1_g000001 Rmu_co8156128.1_g000001 Rmu_co8219154.1_g000003 Rmu_co8297235.1_g000001 Rmu_co8345223.1_g000001 Rmu_co8364775.1_g000001 Rmu_co8465075.1_g000001 Rmu_sc0000007.1_g000002 Rmu_sc0000054.1_g000015 Rmu_sc0000054.1_g000023 Rmu_sc0000145.1_g000010 Rmu_sc0000166.1_g000006 Rmu_sc0000218.1_g000012 Rmu_sc0000339.1_g000008 Rmu_sc0000342.1_g000015 Rmu_sc0000464.1_g000008 Rmu_sc0000620.1_g000003 Rmu_sc0000638.1_g000017 Rmu_sc0000890.1_g000007 Rmu_sc0000894.1_g000024 Rmu_sc0000909.1_g000010 Rmu_sc0000928.1_g000020 Rmu_sc0000977.1_g000007 Rmu_sc0001022.1_g000003 Rmu_sc0001035.1_g000037 Rmu_sc0001060.1_g000008 Rmu_sc0001168.1_g000005 Rmu_sc0001173.1_g000022 Rmu_sc0001179.1_g000016 Rmu_sc0001408.1_g000003 Rmu_sc0001455.1_g000005 Rmu_sc0001484.1_g000008 Rmu_sc0001585.1_g000010 Rmu_sc0001702.1_g000030 Rmu_sc0001755.1_g000063 Rmu_sc0001903.1_g000009 Rmu_sc0002099.1_g000001 Rmu_sc0002142.1_g000012 Rmu_sc0002158.1_g000014 Rmu_sc0002307.1_g000009 Rmu_sc0002358.1_g000013 Rmu_sc0002421.1_g000002 Rmu_sc0002426.1_g000003 Rmu_sc0002438.1_g000001 Rmu_sc0002461.1_g000001 Rmu_sc0002508.1_g000003 Rmu_sc0002532.1_g000056 Rmu_sc0002583.1_g000002 Rmu_sc0002927.1_g000033 Rmu_sc0003033.1_g000040 Rmu_sc0003271.1_g000006 Rmu_sc0003381.1_g000006 Rmu_sc0003507.1_g000007 Rmu_sc0003578.1_g000003 Rmu_sc0003693.1_g000004 Rmu_sc0003693.1_g000005 Rmu_sc0003704.1_g000003 Rmu_sc0003840.1_g000002 Rmu_sc0004084.1_g000026 Rmu_sc0004193.1_g000007 Rmu_sc0004234.1_g000033 Rmu_sc0004235.1_g000025 Rmu_sc0004435.1_g000006 Rmu_sc0004727.1_g000003 Rmu_sc0004741.1_g000001 Rmu_sc0004926.1_g000004 Rmu_sc0005365.1_g000001 Rmu_sc0005392.1_g000007 Rmu_sc0005415.1_g000001 Rmu_sc0005539.1_g000007 Rmu_sc0005708.1_g000017 Rmu_sc0006317.1_g000007 Rmu_sc0006453.1_g000054 Rmu_sc0006536.1_g000004 Rmu_sc0006740.1_g000022 Rmu_sc0006763.1_g000012 Rmu_sc0007115.1_g000002 Rmu_sc0007115.1_g000010 Rmu_sc0007129.1_g000017 Rmu_sc0007131.1_g000010 Rmu_sc0008373.1_g000008 Rmu_sc0009005.1_g000008 Rmu_sc0009252.1_g000007 Rmu_sc0009599.1_g000022 Rmu_sc0009785.1_g000027 Rmu_sc0009891.1_g000003 Rmu_sc0010099.1_g000010 Rmu_sc0010266.1_g000008 Rmu_sc0010334.1_g000003 Rmu_sc0010381.1_g000001 Rmu_sc0010496.1_g000003 Rmu_sc0010496.1_g000004 Rmu_sc0010546.1_g000003 Rmu_sc0011482.1_g000006 Rmu_sc0011875.1_g000010 Rmu_sc0011917.1_g000006 Rmu_sc0012293.1_g000004 Rmu_sc0012847.1_g000003 Rmu_sc0013773.1_g000001 Rmu_sc0016060.1_g000003 Rmu_sc0016170.1_g000013 Rmu_sc0016393.1_g000002 Rmu_sc0017589.1_g000001 Rmu_sc0017786.1_g000005 Rmu_sc0018326.1_g000002 Rmu_sc0020760.1_g000001 Rmu_sc0021262.1_g000003 Rmu_sc0023851.1_g000002 Rmu_sc0024044.1_g000001 Rmu_sc0026820.1_g000001 Rmu_sc0032306.1_g000001 Rmu_sc0035666.1_g000001 Rmu_sc0036881.1_g000001 Rmu_sc0037030.1_g000001 Rmu_sc0037158.1_g000001 Rmu_ssc0000016.1_g000002 Rmu_ssc0000044.1_g000012 Rmu_ssc0000044.1_g000014 Rmu_ssc0000050.1_g000064 Rmu_ssc0000064.1_g000022 Rmu_ssc0000154.1_g000037 Rmu_ssc0000178.1_g000013 Rmu_ssc0000217.1_g000022 Rmu_ssc0000392.1_g000004
rosa_roxburghii Rroxscaffold_3G00242000 Rroxscaffold_3G00242530 Rroxscaffold_6G00406010
rosa_rugosa Rorug01G0062000 Rorug02G0224000 Rorug02G0262900 Rorug02G0263000 Rorug02G0358900 Rorug02G0360500 Rorug02G0378500.1 Rorug02G0378600 Rorug02G0394700 Rorug03G0122900 Rorug03G0254600 Rorug03G0254700 Rorug03G0254800 Rorug04G0042200.1 Rorug04G0378100 Rorug05G0053400 Rorug05G0238600 Rorug05G0364500 Rorug05G0562000 Rorug06G0089300 Rorug06G0089400 Rorug06G0145700 Rorug07G0111600 Rorug07G0229500.1 Rorug07G0287600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2762
Acc36I ACCTGC 1 cut(s) 2762
AccB1I GGYRCC 2 cut(s) 1081, 1684
AciI CCGC 2 cut(s) 355, 1386
AclWI GGATC 8 cut(s) 1457, 1819, 1842, 2141, 2464, 2562, 2575, 2627
AcoI YGGCCR 1 cut(s) 2933
AcsI RAATTY 9 cut(s) 272, 761, 978, 1314, 1614, 2522, 2532, 2954, 3101
AfaI GTAC 5 cut(s) 601, 1845, 2211, 2382, 3031
AfeI AGCGCT 1 cut(s) 2734
AfiI CCNNNNNNNGG 2 cut(s) 111, 136
AflII CTTAAG 1 cut(s) 2441
AflIII ACRYGT 2 cut(s) 774, 2383
AhdI GACNNNNNGTC 2 cut(s) 775, 2355
AjnI CCWGG 2 cut(s) 389, 2079
AleI CACNNNNGTG 1 cut(s) 1112
AluBI AGCT 9 cut(s) 30, 164, 969, 1505, 1631, 2154, 2502, 2552, 2585
AluI AGCT 9 cut(s) 30, 164, 969, 1505, 1631, 2154, 2502, 2552, 2585
Alw21I GWGCWC 3 cut(s) 136, 3075, 3123
Alw26I GTCTC 5 cut(s) 20, 527, 1706, 1760, 1884
Alw44I GTGCAC 1 cut(s) 3071
AlwI GGATC 8 cut(s) 1457, 1819, 1842, 2141, 2464, 2562, 2575, 2627
AlwNI CAGNNNCTG 1 cut(s) 2465
Aor51HI AGCGCT 1 cut(s) 2734
AoxI GGCC 2 cut(s) 393, 2933
ApaLI GTGCAC 1 cut(s) 3071
ApeKI GCWGC 5 cut(s) 905, 1196, 2076, 2465, 2502
ApoI RAATTY 9 cut(s) 272, 761, 978, 1314, 1614, 2522, 2532, 2954, 3101
Asp700I GAANNNNTTC 2 cut(s) 2290, 2957
AspLEI GCGC 2 cut(s) 753, 2735
AspS9I GGNCC 6 cut(s) 34, 393, 635, 1509, 1625, 2915
AsuHPI GGTGA 2 cut(s) 2005, 2071
AsuII TTCGAA 2 cut(s) 159, 1187
AvaII GGWCC 5 cut(s) 34, 635, 1509, 1625, 2915
BaeGI GKGCMC 2 cut(s) 1084, 3075
BaeI ACNNNNGTAYC 2 cut(s) 2097, 2130
BalI TGGCCA 1 cut(s) 2935
BamHI GGATCC 1 cut(s) 2567
BanI GGYRCC 2 cut(s) 1081, 1684
BanII GRGCYC 1 cut(s) 829
BbsI GAAGAC 2 cut(s) 51, 451
Bbv12I GWGCWC 3 cut(s) 136, 3075, 3123
BbvI GCAGC 5 cut(s) 892, 1208, 2088, 2452, 2489
BccI CCATC 8 cut(s) 139, 402, 566, 881, 1520, 1802, 2039, 2447
BceAI ACGGC 1 cut(s) 1672
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BciT130I CCWGG 2 cut(s) 391, 2081
BciVI GTATCC 2 cut(s) 2020, 2098
BclI TGATCA 2 cut(s) 1234, 1965
BcoDI GTCTC 5 cut(s) 20, 527, 1706, 1760, 1884
BfaI CTAG 5 cut(s) 375, 425, 846, 1179, 1878
BfmI CTRYAG 1 cut(s) 906
BfoI RGCGCY 1 cut(s) 2736
BfrI CTTAAG 1 cut(s) 2441
BfuAI ACCTGC 1 cut(s) 2762
BfuI GTATCC 2 cut(s) 2020, 2098
BglII AGATCT 3 cut(s) 1170, 1588, 2682
BisI GCNGC 5 cut(s) 906, 1197, 2077, 2466, 2503
BlsI GCNGC 5 cut(s) 907, 1198, 2078, 2467, 2504
Bme1390I CCNGG 2 cut(s) 391, 2081
Bme18I GGWCC 5 cut(s) 34, 635, 1509, 1625, 2915
BmeRI GACNNNNNGTC 2 cut(s) 775, 2355
BmgT120I GGNCC 6 cut(s) 34, 393, 635, 1509, 1625, 2915
BmiI GGNNCC 7 cut(s) 220, 388, 1083, 1510, 1686, 1860, 2569
BmrFI CCNGG 2 cut(s) 391, 2081
BmrI ACTGGG 1 cut(s) 1917
BmsI GCATC 9 cut(s) 631, 1012, 1034, 1732, 2245, 2267, 2477, 2656, 2758
BmuI ACTGGG 1 cut(s) 1917
BoxI GACNNNNGTC 1 cut(s) 1284
BpiI GAAGAC 2 cut(s) 51, 451
BplI GAGNNNNNCTC 4 cut(s) 1160, 1192, 1757, 1789
BpmI CTGGAG 2 cut(s) 2608, 2852
Bpu14I TTCGAA 2 cut(s) 159, 1187
Bsa29I ATCGAT 2 cut(s) 2493, 3025
BsaBI GATNNNNATC 1 cut(s) 872
BsaI GGTCTC 3 cut(s) 527, 1706, 1760
BsaJI CCNNGG 3 cut(s) 390, 1599, 1671
BsaWI WCCGGW 2 cut(s) 136, 2554
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 136
Bse1I ACTGG 8 cut(s) 983, 1210, 1912, 2533, 2625, 2741, 2892, 2936
Bse3DI GCAATG 1 cut(s) 1237
Bse8I GATNNNNATC 1 cut(s) 872
BseBI CCWGG 2 cut(s) 391, 2081
BseCI ATCGAT 2 cut(s) 2493, 3025
BseDI CCNNGG 3 cut(s) 390, 1599, 1671
BseGI GGATG 6 cut(s) 413, 1103, 1378, 1723, 2406, 2468
BseJI GATNNNNATC 1 cut(s) 872
BseLI CCNNNNNNNGG 2 cut(s) 111, 136
BseMI GCAATG 1 cut(s) 1237
BseMII CTCAG 7 cut(s) 194, 984, 1058, 1623, 1638, 1701, 2268
BseNI ACTGG 8 cut(s) 983, 1210, 1912, 2533, 2625, 2741, 2892, 2936
BseSI GKGCMC 2 cut(s) 1084, 3075
BseXI GCAGC 5 cut(s) 892, 1208, 2088, 2452, 2489
BseYI CCCAGC 1 cut(s) 1505
BsgI GTGCAG 1 cut(s) 189
BshFI GGCC 2 cut(s) 395, 2935
BshNI GGYRCC 2 cut(s) 1081, 1684
BshVI ATCGAT 2 cut(s) 2493, 3025
BsiHKAI GWGCWC 3 cut(s) 136, 3075, 3123
BsiSI CCGG 3 cut(s) 137, 1798, 2555
BslFI GGGAC 2 cut(s) 1522, 1657
BslI CCNNNNNNNGG 2 cut(s) 111, 136
BsmAI GTCTC 5 cut(s) 20, 527, 1706, 1760, 1884
BsmFI GGGAC 2 cut(s) 1522, 1657
BsmI GAATGC 4 cut(s) 418, 1799, 1807, 1952
BsnI GGCC 2 cut(s) 395, 2935
Bso31I GGTCTC 3 cut(s) 527, 1706, 1760
Bsp119I TTCGAA 2 cut(s) 159, 1187
Bsp1286I GDGCHC 5 cut(s) 136, 829, 1084, 3075, 3123
Bsp1407I TGTACA 1 cut(s) 2380
BspACI CCGC 2 cut(s) 355, 1386
BspANI GGCC 2 cut(s) 395, 2935
BspCNI CTCAG 7 cut(s) 193, 983, 1057, 1624, 1639, 1702, 2269
BspDI ATCGAT 2 cut(s) 2493, 3025
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 7 cut(s) 220, 388, 1083, 1510, 1686, 1860, 2569
BspMAI CTGCAG 1 cut(s) 910
BspMI ACCTGC 1 cut(s) 2762
BspPI GGATC 8 cut(s) 1457, 1819, 1842, 2141, 2464, 2562, 2575, 2627
BspQI GCTCTTC 1 cut(s) 2742
BspT104I TTCGAA 2 cut(s) 159, 1187
BspT107I GGYRCC 2 cut(s) 1081, 1684
BspTI CTTAAG 1 cut(s) 2441
BspTNI GGTCTC 3 cut(s) 527, 1706, 1760
BsrDI GCAATG 1 cut(s) 1237
BsrGI TGTACA 1 cut(s) 2380
BsrI ACTGG 8 cut(s) 983, 1210, 1912, 2533, 2625, 2741, 2892, 2936
BssECI CCNNGG 3 cut(s) 390, 1599, 1671
BssT1I CCWWGG 2 cut(s) 1599, 1671
Bst2UI CCWGG 2 cut(s) 391, 2081
Bst4CI ACNGT 4 cut(s) 264, 490, 604, 2819
Bst6I CTCTTC 5 cut(s) 135, 1161, 2118, 2742, 3105
BstAFI CTTAAG 1 cut(s) 2441
BstAPI GCANNNNNTGC 1 cut(s) 764
BstAUI TGTACA 1 cut(s) 2380
BstBI TTCGAA 2 cut(s) 159, 1187
BstC8I GCNNGC 2 cut(s) 749, 2223
BstDEI CTNAG 8 cut(s) 180, 970, 1044, 1632, 1647, 1710, 2277, 2412
BstF5I GGATG 6 cut(s) 413, 1103, 1378, 1723, 2406, 2468
BstH2I RGCGCY 1 cut(s) 2736
BstHHI GCGC 2 cut(s) 753, 2735
BstMAI GTCTC 5 cut(s) 20, 527, 1706, 1760, 1884
BstMWI GCNNNNNNNGC 1 cut(s) 764
BstNI CCWGG 2 cut(s) 391, 2081
BstNSI RCATGY 2 cut(s) 778, 2387
BstPAI GACNNNNGTC 1 cut(s) 1284
BstSCI CCNGG 2 cut(s) 389, 2079
BstSFI CTRYAG 1 cut(s) 906
BstSLI GKGCMC 2 cut(s) 1084, 3075
BstV1I GCAGC 5 cut(s) 892, 1208, 2088, 2452, 2489
BstV2I GAAGAC 2 cut(s) 51, 451
BstX2I RGATCY 7 cut(s) 1170, 1462, 1588, 2146, 2567, 2632, 2682
BstXI CCANNNNNNTGG 1 cut(s) 590
BstYI RGATCY 7 cut(s) 1170, 1462, 1588, 2146, 2567, 2632, 2682
Bsu15I ATCGAT 2 cut(s) 2493, 3025
BsuI GTATCC 2 cut(s) 2020, 2098
BsuRI GGCC 2 cut(s) 395, 2935
BsuTUI ATCGAT 2 cut(s) 2493, 3025
BtsCI GGATG 6 cut(s) 413, 1103, 1378, 1723, 2406, 2468
BtsI GCAGTG 2 cut(s) 889, 915
BtsIMutI CAGTG 9 cut(s) 584, 609, 889, 915, 944, 990, 1707, 2466, 2748
BveI ACCTGC 1 cut(s) 2762
Cac8I GCNNGC 2 cut(s) 749, 2223
CaiI CAGNNNCTG 1 cut(s) 2465
CciI TCATGA 1 cut(s) 151
CfoI GCGC 2 cut(s) 753, 2735
Cfr13I GGNCC 6 cut(s) 34, 393, 635, 1509, 1625, 2915
ClaI ATCGAT 2 cut(s) 2493, 3025
Csp6I GTAC 5 cut(s) 600, 1844, 2210, 2381, 3030
CspCI CAANNNNNGTGG 2 cut(s) 1585, 1620
CviQI GTAC 5 cut(s) 600, 1844, 2210, 2381, 3030
DdeI CTNAG 8 cut(s) 180, 970, 1044, 1632, 1647, 1710, 2277, 2412
DriI GACNNNNNGTC 2 cut(s) 775, 2355
EaeI YGGCCR 1 cut(s) 2933
Eam1104I CTCTTC 5 cut(s) 135, 1161, 2118, 2742, 3105
Eam1105I GACNNNNNGTC 2 cut(s) 775, 2355
EarI CTCTTC 5 cut(s) 135, 1161, 2118, 2742, 3105
Eco130I CCWWGG 2 cut(s) 1599, 1671
Eco24I GRGCYC 1 cut(s) 829
Eco31I GGTCTC 3 cut(s) 527, 1706, 1760
Eco47I GGWCC 5 cut(s) 34, 635, 1509, 1625, 2915
Eco47III AGCGCT 1 cut(s) 2734
EcoO109I RGGNCCY 1 cut(s) 2915
EcoRI GAATTC 1 cut(s) 3101
EcoRII CCWGG 2 cut(s) 389, 2079
EcoT14I CCWWGG 2 cut(s) 1599, 1671
EcoT22I ATGCAT 2 cut(s) 1027, 2260
EcoT38I GRGCYC 1 cut(s) 829
ErhI CCWWGG 2 cut(s) 1599, 1671
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FaqI GGGAC 2 cut(s) 1522, 1657
FauNDI CATATG 1 cut(s) 3062
FbaI TGATCA 2 cut(s) 1234, 1965
Fnu4HI GCNGC 5 cut(s) 906, 1197, 2077, 2466, 2503
FokI GGATG 6 cut(s) 420, 1090, 1365, 1710, 2393, 2455
FriOI GRGCYC 1 cut(s) 829
Fsp4HI GCNGC 5 cut(s) 906, 1197, 2077, 2466, 2503
FspBI CTAG 5 cut(s) 375, 425, 846, 1179, 1878
GlaI GCGC 2 cut(s) 752, 2734
GluI GCNGC 5 cut(s) 906, 1197, 2077, 2466, 2503
GsaI CCCAGC 1 cut(s) 1509
GsuI CTGGAG 2 cut(s) 2608, 2852
HaeII RGCGCY 1 cut(s) 2736
HaeIII GGCC 2 cut(s) 395, 2935
HapII CCGG 3 cut(s) 137, 1798, 2555
HhaI GCGC 2 cut(s) 753, 2735
Hin6I GCGC 2 cut(s) 751, 2733
HinP1I GCGC 2 cut(s) 751, 2733
HincII GTYRAC 1 cut(s) 1371
HindII GTYRAC 1 cut(s) 1371
HindIII AAGCTT 1 cut(s) 162
HpaII CCGG 3 cut(s) 137, 1798, 2555
HphI GGTGA 2 cut(s) 2005, 2071
Hpy166II GTNNAC 8 cut(s) 350, 479, 565, 1371, 1534, 2212, 2620, 3073
Hpy8I GTNNAC 8 cut(s) 350, 479, 565, 1371, 1534, 2212, 2620, 3073
Hpy99I CGWCG 1 cut(s) 2117
HpyCH4III ACNGT 4 cut(s) 264, 490, 604, 2819
HpyCH4IV ACGT 2 cut(s) 468, 2374
HpyF10VI GCNNNNNNNGC 1 cut(s) 764
HpyF3I CTNAG 8 cut(s) 180, 970, 1044, 1632, 1647, 1710, 2277, 2412
HpySE526I ACGT 2 cut(s) 468, 2374
HspAI GCGC 2 cut(s) 751, 2733
Ksp22I TGATCA 2 cut(s) 1234, 1965
LguI GCTCTTC 1 cut(s) 2742
LmnI GCTCC 1 cut(s) 2557
Lsp1109I GCAGC 5 cut(s) 892, 1208, 2088, 2452, 2489
LweI GCATC 9 cut(s) 631, 1012, 1034, 1732, 2245, 2267, 2477, 2656, 2758
MaeI CTAG 5 cut(s) 375, 425, 846, 1179, 1878
MaeII ACGT 2 cut(s) 468, 2374
MaeIII GTNAC 5 cut(s) 1114, 1655, 1993, 2059, 3040
MflI RGATCY 7 cut(s) 1170, 1462, 1588, 2146, 2567, 2632, 2682
MhlI GDGCHC 5 cut(s) 136, 829, 1084, 3075, 3123
MlsI TGGCCA 1 cut(s) 2935
MluNI TGGCCA 1 cut(s) 2935
MlyI GAGTC 6 cut(s) 1036, 1451, 1928, 1991, 2135, 2710
MmeI TCCRAC 3 cut(s) 1160, 1585, 2098
Mox20I TGGCCA 1 cut(s) 2935
Mph1103I ATGCAT 2 cut(s) 1027, 2260
MroXI GAANNNNTTC 2 cut(s) 2290, 2957
MscI TGGCCA 1 cut(s) 2935
MseI TTAA 9 cut(s) 381, 492, 975, 1868, 2192, 2301, 2442, 2559, 3081
MslI CAYNNNNRTG 6 cut(s) 78, 570, 723, 1112, 1381, 2382
Msp20I TGGCCA 1 cut(s) 2935
MspA1I CMGCKG 2 cut(s) 1631, 2502
MspCI CTTAAG 1 cut(s) 2441
MspI CCGG 3 cut(s) 137, 1798, 2555
MspR9I CCNGG 2 cut(s) 391, 2081
Mva1269I GAATGC 4 cut(s) 418, 1799, 1807, 1952
MvaI CCWGG 2 cut(s) 391, 2081
MwoI GCNNNNNNNGC 1 cut(s) 764
NdeI CATATG 1 cut(s) 3062
NlaIV GGNNCC 7 cut(s) 220, 388, 1083, 1510, 1686, 1860, 2569
NmuCI GTSAC 4 cut(s) 1114, 1993, 2059, 3040
NsiI ATGCAT 2 cut(s) 1027, 2260
NspI RCATGY 2 cut(s) 778, 2387
NspV TTCGAA 2 cut(s) 159, 1187
OliI CACNNNNGTG 1 cut(s) 1112
PagI TCATGA 1 cut(s) 151
PaqCI CACCTGC 1 cut(s) 2762
PciI ACATGT 2 cut(s) 774, 2383
PciSI GCTCTTC 1 cut(s) 2742
PctI GAATGC 4 cut(s) 418, 1799, 1807, 1952
PdmI GAANNNNTTC 2 cut(s) 2290, 2957
PfeI GAWTC 9 cut(s) 196, 341, 523, 854, 938, 1100, 1580, 1700, 2186
PkrI GCNGC 5 cut(s) 907, 1198, 2078, 2467, 2504
PleI GAGTC 6 cut(s) 1036, 1450, 1927, 1990, 2134, 2709
PpsI GAGTC 6 cut(s) 1036, 1450, 1927, 1990, 2134, 2709
PpuMI RGGWCCY 1 cut(s) 2915
PscI ACATGT 2 cut(s) 774, 2383
PshAI GACNNNNGTC 1 cut(s) 1284
Psp5II RGGWCCY 1 cut(s) 2915
Psp6I CCWGG 2 cut(s) 389, 2079
PspFI CCCAGC 1 cut(s) 1505
PspGI CCWGG 2 cut(s) 389, 2079
PspN4I GGNNCC 7 cut(s) 220, 388, 1083, 1510, 1686, 1860, 2569
PspPI GGNCC 6 cut(s) 34, 393, 635, 1509, 1625, 2915
PspPPI RGGWCCY 1 cut(s) 2915
PstI CTGCAG 1 cut(s) 910
PstNI CAGNNNCTG 1 cut(s) 2465
PsuI RGATCY 7 cut(s) 1170, 1462, 1588, 2146, 2567, 2632, 2682
PvuII CAGCTG 2 cut(s) 1631, 2502
RsaI GTAC 5 cut(s) 601, 1845, 2211, 2382, 3031
RsaNI GTAC 5 cut(s) 600, 1844, 2210, 2381, 3030
RseI CAYNNNNRTG 6 cut(s) 78, 570, 723, 1112, 1381, 2382
SapI GCTCTTC 1 cut(s) 2742
SaqAI TTAA 9 cut(s) 381, 492, 975, 1868, 2192, 2301, 2442, 2559, 3081
SatI GCNGC 5 cut(s) 906, 1197, 2077, 2466, 2503
Sau96I GGNCC 6 cut(s) 34, 393, 635, 1509, 1625, 2915
SchI GAGTC 6 cut(s) 1036, 1451, 1928, 1991, 2135, 2710
ScrFI CCNGG 2 cut(s) 391, 2081
SduI GDGCHC 5 cut(s) 136, 829, 1084, 3075, 3123
SfaNI GCATC 9 cut(s) 631, 1012, 1034, 1732, 2245, 2267, 2477, 2656, 2758
SfcI CTRYAG 1 cut(s) 906
SfuI TTCGAA 2 cut(s) 159, 1187
SinI GGWCC 5 cut(s) 34, 635, 1509, 1625, 2915
SmiMI CAYNNNNRTG 6 cut(s) 78, 570, 723, 1112, 1381, 2382
SmlI CTYRAG 1 cut(s) 2441
SmoI CTYRAG 1 cut(s) 2441
SsiI CCGC 2 cut(s) 355, 1386
SspI AATATT 1 cut(s) 2576
SspMI CTAG 5 cut(s) 375, 425, 846, 1179, 1878
StyD4I CCNGG 2 cut(s) 389, 2079
StyI CCWWGG 2 cut(s) 1599, 1671
TaaI ACNGT 4 cut(s) 264, 490, 604, 2819
TaiI ACGT 2 cut(s) 471, 2377
TaqI TCGA 5 cut(s) 159, 228, 1187, 2493, 3025
TatI WGTACW 3 cut(s) 599, 2380, 3029
TfiI GAWTC 9 cut(s) 196, 341, 523, 854, 938, 1100, 1580, 1700, 2186
Tru1I TTAA 9 cut(s) 381, 492, 975, 1868, 2192, 2301, 2442, 2559, 3081
Tru9I TTAA 9 cut(s) 381, 492, 975, 1868, 2192, 2301, 2442, 2559, 3081
TscAI CASTG 9 cut(s) 584, 609, 889, 915, 951, 990, 1714, 2466, 2748
TseFI GTSAC 4 cut(s) 1114, 1993, 2059, 3040
TseI GCWGC 5 cut(s) 905, 1196, 2076, 2465, 2502
Tsp45I GTSAC 4 cut(s) 1114, 1993, 2059, 3040
TspGWI ACGGA 1 cut(s) 604
TspRI CASTG 9 cut(s) 584, 609, 889, 915, 951, 990, 1714, 2466, 2748
Vha464I CTTAAG 1 cut(s) 2441
VneI GTGCAC 1 cut(s) 3071
VpaK11BI GGWCC 5 cut(s) 34, 635, 1509, 1625, 2915
XapI RAATTY 9 cut(s) 272, 761, 978, 1314, 1614, 2522, 2532, 2954, 3101
XceI RCATGY 2 cut(s) 778, 2387
XmnI GAANNNNTTC 2 cut(s) 2290, 2957
XspI CTAG 5 cut(s) 375, 425, 846, 1179, 1878
Zsp2I ATGCAT 2 cut(s) 1027, 2260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.