Rmu_co8297235.1_g000001

Zinc knuckle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8297235.1
OG0000187
Physical Location & Seq
Reverse (-)
413 .. 865
453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8297235.1_g000001.1.cds

Sequence Viewer

Length: 453 bp
atgcgacaggcaaacgtgtttatggtggggaagcttctcaccgctcgggagtacaagaatcgatctttcctggggatgttccggaatgcatggagggtgaatggttcactacgcgtcgaggaggcggagtggcagagggttcttgtcactctatctgacgctaccggtcgggatcggatctggaagggagctccatggggttacaatcatgctccggttgccctagcctactacgatggcgtggctactattgaatcggtatctttcacctcagccacgttctggatcactttgcaaggcatccctcttgcgtttcgttctgagagggcgatgattatgatcgggttcaacctgggcgacttcaaggagattgacaaacaggccaagaaggttgggaagcttcgaatccgggtggaaatccctcttaaccggcctctgccctttcaacgatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

150

Amino Acids

17.24

Weight (kDa)

10.41

Isoelectric Point (pI)

31.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000187)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20170 FvH4_4g06831
rosa_chinensis RchiOBHm_Chr1g0321991 RchiOBHm_Chr2g0161811
rosa_laevigata RLG00000015375 RLG00000018377
rosa_multiflora Rmu_co8011754.1_g000001 Rmu_co8019986.1_g000001 Rmu_co8067766.1_g000001 Rmu_co8156128.1_g000001 Rmu_co8219154.1_g000003 Rmu_co8297235.1_g000001 Rmu_co8345223.1_g000001 Rmu_co8364775.1_g000001 Rmu_co8465075.1_g000001 Rmu_sc0000007.1_g000002 Rmu_sc0000054.1_g000015 Rmu_sc0000054.1_g000023 Rmu_sc0000145.1_g000010 Rmu_sc0000166.1_g000006 Rmu_sc0000218.1_g000012 Rmu_sc0000339.1_g000008 Rmu_sc0000342.1_g000015 Rmu_sc0000464.1_g000008 Rmu_sc0000620.1_g000003 Rmu_sc0000638.1_g000017 Rmu_sc0000890.1_g000007 Rmu_sc0000894.1_g000024 Rmu_sc0000909.1_g000010 Rmu_sc0000928.1_g000020 Rmu_sc0000977.1_g000007 Rmu_sc0001022.1_g000003 Rmu_sc0001035.1_g000037 Rmu_sc0001060.1_g000008 Rmu_sc0001168.1_g000005 Rmu_sc0001173.1_g000022 Rmu_sc0001179.1_g000016 Rmu_sc0001408.1_g000003 Rmu_sc0001455.1_g000005 Rmu_sc0001484.1_g000008 Rmu_sc0001585.1_g000010 Rmu_sc0001702.1_g000030 Rmu_sc0001755.1_g000063 Rmu_sc0001903.1_g000009 Rmu_sc0002099.1_g000001 Rmu_sc0002142.1_g000012 Rmu_sc0002158.1_g000014 Rmu_sc0002307.1_g000009 Rmu_sc0002358.1_g000013 Rmu_sc0002421.1_g000002 Rmu_sc0002426.1_g000003 Rmu_sc0002438.1_g000001 Rmu_sc0002461.1_g000001 Rmu_sc0002508.1_g000003 Rmu_sc0002532.1_g000056 Rmu_sc0002583.1_g000002 Rmu_sc0002927.1_g000033 Rmu_sc0003033.1_g000040 Rmu_sc0003271.1_g000006 Rmu_sc0003381.1_g000006 Rmu_sc0003507.1_g000007 Rmu_sc0003578.1_g000003 Rmu_sc0003693.1_g000004 Rmu_sc0003693.1_g000005 Rmu_sc0003704.1_g000003 Rmu_sc0003840.1_g000002 Rmu_sc0004084.1_g000026 Rmu_sc0004193.1_g000007 Rmu_sc0004234.1_g000033 Rmu_sc0004235.1_g000025 Rmu_sc0004435.1_g000006 Rmu_sc0004727.1_g000003 Rmu_sc0004741.1_g000001 Rmu_sc0004926.1_g000004 Rmu_sc0005365.1_g000001 Rmu_sc0005392.1_g000007 Rmu_sc0005415.1_g000001 Rmu_sc0005539.1_g000007 Rmu_sc0005708.1_g000017 Rmu_sc0006317.1_g000007 Rmu_sc0006453.1_g000054 Rmu_sc0006536.1_g000004 Rmu_sc0006740.1_g000022 Rmu_sc0006763.1_g000012 Rmu_sc0007115.1_g000002 Rmu_sc0007115.1_g000010 Rmu_sc0007129.1_g000017 Rmu_sc0007131.1_g000010 Rmu_sc0008373.1_g000008 Rmu_sc0009005.1_g000008 Rmu_sc0009252.1_g000007 Rmu_sc0009599.1_g000022 Rmu_sc0009785.1_g000027 Rmu_sc0009891.1_g000003 Rmu_sc0010099.1_g000010 Rmu_sc0010266.1_g000008 Rmu_sc0010334.1_g000003 Rmu_sc0010381.1_g000001 Rmu_sc0010496.1_g000003 Rmu_sc0010496.1_g000004 Rmu_sc0010546.1_g000003 Rmu_sc0011482.1_g000006 Rmu_sc0011875.1_g000010 Rmu_sc0011917.1_g000006 Rmu_sc0012293.1_g000004 Rmu_sc0012847.1_g000003 Rmu_sc0013773.1_g000001 Rmu_sc0016060.1_g000003 Rmu_sc0016170.1_g000013 Rmu_sc0016393.1_g000002 Rmu_sc0017589.1_g000001 Rmu_sc0017786.1_g000005 Rmu_sc0018326.1_g000002 Rmu_sc0020760.1_g000001 Rmu_sc0021262.1_g000003 Rmu_sc0023851.1_g000002 Rmu_sc0024044.1_g000001 Rmu_sc0026820.1_g000001 Rmu_sc0032306.1_g000001 Rmu_sc0035666.1_g000001 Rmu_sc0036881.1_g000001 Rmu_sc0037030.1_g000001 Rmu_sc0037158.1_g000001 Rmu_ssc0000016.1_g000002 Rmu_ssc0000044.1_g000012 Rmu_ssc0000044.1_g000014 Rmu_ssc0000050.1_g000064 Rmu_ssc0000064.1_g000022 Rmu_ssc0000154.1_g000037 Rmu_ssc0000178.1_g000013 Rmu_ssc0000217.1_g000022 Rmu_ssc0000392.1_g000004
rosa_roxburghii Rroxscaffold_3G00242000 Rroxscaffold_3G00242530 Rroxscaffold_6G00406010
rosa_rugosa Rorug01G0062000 Rorug02G0224000 Rorug02G0262900 Rorug02G0263000 Rorug02G0358900 Rorug02G0360500 Rorug02G0378500.1 Rorug02G0378600 Rorug02G0394700 Rorug03G0122900 Rorug03G0254600 Rorug03G0254700 Rorug03G0254800 Rorug04G0042200.1 Rorug04G0378100 Rorug05G0053400 Rorug05G0238600 Rorug05G0364500 Rorug05G0562000 Rorug06G0089300 Rorug06G0089400 Rorug06G0145700 Rorug07G0111600 Rorug07G0229500.1 Rorug07G0287600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 282
AccBSI CCGCTC 1 cut(s) 44
AccII CGCG 1 cut(s) 114
AccIII TCCGGA 1 cut(s) 81
AciI CCGC 2 cut(s) 42, 125
AclWI GGATC 3 cut(s) 180, 185, 293
AfaI GTAC 1 cut(s) 53
AfiI CCNNNNNNNGG 1 cut(s) 282
AflIII ACRYGT 2 cut(s) 15, 112
AgeI ACCGGT 1 cut(s) 164
AgsI TTSAA 4 cut(s) 254, 349, 364, 446
AjnI CCWGG 2 cut(s) 69, 351
AluBI AGCT 3 cut(s) 34, 191, 400
AluI AGCT 3 cut(s) 34, 191, 400
Alw21I GWGCWC 1 cut(s) 193
AlwI GGATC 3 cut(s) 180, 185, 293
Ama87I CYCGRG 1 cut(s) 45
Aor13HI TCCGGA 1 cut(s) 81
AoxI GGCC 2 cut(s) 381, 431
AsiGI ACCGGT 1 cut(s) 164
AsuC2I CCSGG 1 cut(s) 410
AsuHPI GGTGA 3 cut(s) 31, 109, 259
AsuII TTCGAA 1 cut(s) 403
AvaI CYCGRG 1 cut(s) 45
BanII GRGCYC 1 cut(s) 193
Bbv12I GWGCWC 1 cut(s) 193
BbvCI CCTCAGC 1 cut(s) 271
BccI CCATC 1 cut(s) 230
BciT130I CCWGG 2 cut(s) 71, 353
BcnI CCSGG 1 cut(s) 410
BfaI CTAG 1 cut(s) 224
Bme1390I CCNGG 3 cut(s) 71, 353, 410
BmeT110I CYCGRG 1 cut(s) 45
BmrFI CCNGG 3 cut(s) 71, 353, 410
BmsI GCATC 1 cut(s) 309
Bpu10I CCTNAGC 1 cut(s) 271
Bpu14I TTCGAA 1 cut(s) 403
BpuMI CCSGG 1 cut(s) 410
Bsa29I ATCGAT 1 cut(s) 61
BsaBI GATNNNNATC 1 cut(s) 338
BsaJI CCNNGG 3 cut(s) 70, 194, 352
BsaWI WCCGGW 3 cut(s) 81, 164, 214
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 1 cut(s) 282
Bse118I RCCGGY 2 cut(s) 164, 429
Bse8I GATNNNNATC 1 cut(s) 338
BseAI TCCGGA 1 cut(s) 81
BseBI CCWGG 2 cut(s) 71, 353
BseCI ATCGAT 1 cut(s) 61
BseDI CCNNGG 3 cut(s) 70, 194, 352
BseGI GGATG 2 cut(s) 81, 300
BseJI GATNNNNATC 1 cut(s) 338
BseLI CCNNNNNNNGG 1 cut(s) 282
BseMII CTCAG 2 cut(s) 285, 312
BseRI GAGGAG 1 cut(s) 134
Bsh1236I CGCG 1 cut(s) 114
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 2 cut(s) 383, 433
BshTI ACCGGT 1 cut(s) 164
BshVI ATCGAT 1 cut(s) 61
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 193
BsiHKCI CYCGRG 1 cut(s) 45
BsiSI CCGG 5 cut(s) 82, 165, 215, 409, 430
BslI CCNNNNNNNGG 1 cut(s) 282
BsmI GAATGC 1 cut(s) 91
BsnI GGCC 2 cut(s) 383, 433
BsoBI CYCGRG 1 cut(s) 45
Bsp119I TTCGAA 1 cut(s) 403
Bsp1286I GDGCHC 1 cut(s) 193
Bsp13I TCCGGA 1 cut(s) 81
Bsp143I GATC 5 cut(s) 62, 172, 177, 285, 339
Bsp19I CCATGG 1 cut(s) 194
BspACI CCGC 2 cut(s) 42, 125
BspANI GGCC 2 cut(s) 383, 433
BspCNI CTCAG 2 cut(s) 284, 313
BspDI ATCGAT 1 cut(s) 61
BspEI TCCGGA 1 cut(s) 81
BspFNI CGCG 1 cut(s) 114
BspPI GGATC 3 cut(s) 180, 185, 293
BspT104I TTCGAA 1 cut(s) 403
BsrBI CCGCTC 1 cut(s) 44
BsrFI RCCGGY 2 cut(s) 164, 429
BssAI RCCGGY 2 cut(s) 164, 429
BssECI CCNNGG 3 cut(s) 70, 194, 352
BssMI GATC 5 cut(s) 62, 172, 177, 285, 339
BssT1I CCWWGG 1 cut(s) 194
Bst2UI CCWGG 2 cut(s) 71, 353
BstBI TTCGAA 1 cut(s) 403
BstDEI CTNAG 2 cut(s) 271, 321
BstDSI CCRYGG 1 cut(s) 194
BstF5I GGATG 2 cut(s) 81, 300
BstFNI CGCG 1 cut(s) 114
BstKTI GATC 5 cut(s) 65, 175, 180, 288, 342
BstMBI GATC 5 cut(s) 62, 172, 177, 285, 339
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstNI CCWGG 2 cut(s) 71, 353
BstSCI CCNGG 3 cut(s) 69, 351, 408
BstUI CGCG 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
Bsu15I ATCGAT 1 cut(s) 61
BsuRI GGCC 2 cut(s) 383, 433
BsuTUI ATCGAT 1 cut(s) 61
BtgI CCRYGG 1 cut(s) 194
BtgZI GCGATG 1 cut(s) 344
BtsCI GGATG 2 cut(s) 81, 300
Cfr10I RCCGGY 2 cut(s) 164, 429
ClaI ATCGAT 1 cut(s) 61
CseI GACGC 2 cut(s) 103, 167
Csp6I GTAC 1 cut(s) 52
CspAI ACCGGT 1 cut(s) 164
CviAII CATG 3 cut(s) 90, 195, 209
CviJI RGCY 8 cut(s) 34, 191, 227, 245, 275, 383, 400, 433
CviKI_1 RGCY 8 cut(s) 34, 191, 227, 245, 275, 383, 400, 433
CviQI GTAC 1 cut(s) 52
DdeI CTNAG 2 cut(s) 271, 321
DpnI GATC 5 cut(s) 64, 174, 179, 287, 341
DpnII GATC 5 cut(s) 62, 172, 177, 285, 339
EciI GGCGGA 1 cut(s) 140
Ecl136II GAGCTC 1 cut(s) 191
Eco130I CCWWGG 1 cut(s) 194
Eco24I GRGCYC 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 191
Eco88I CYCGRG 1 cut(s) 45
EcoICRI GAGCTC 1 cut(s) 191
EcoRII CCWGG 2 cut(s) 69, 351
EcoT14I CCWWGG 1 cut(s) 194
EcoT22I ATGCAT 1 cut(s) 91
EcoT38I GRGCYC 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 194
FaeI CATG 3 cut(s) 93, 198, 212
FaiI YATR 5 cut(s) 23, 91, 196, 210, 338
FatI CATG 3 cut(s) 89, 194, 208
FokI GGATG 2 cut(s) 88, 287
FriOI GRGCYC 1 cut(s) 193
FspBI CTAG 1 cut(s) 224
HaeIII GGCC 2 cut(s) 383, 433
HapII CCGG 5 cut(s) 82, 165, 215, 409, 430
HgaI GACGC 2 cut(s) 103, 167
Hin1II CATG 3 cut(s) 93, 198, 212
HindIII AAGCTT 2 cut(s) 32, 398
HinfI GANTC 3 cut(s) 58, 254, 405
HpaII CCGG 5 cut(s) 82, 165, 215, 409, 430
HphI GGTGA 3 cut(s) 31, 109, 259
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 3 cut(s) 157, 177, 322
Hpy188III TCNNGA 5 cut(s) 47, 82, 170, 181, 283
Hpy8I GTNNAC 1 cut(s) 107
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 2 cut(s) 178, 382
HpyCH4IV ACGT 2 cut(s) 15, 278
HpyCH4V TGCA 2 cut(s) 89, 295
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 2 cut(s) 271, 321
HpySE526I ACGT 2 cut(s) 15, 278
Hsp92II CATG 3 cut(s) 93, 198, 212
Kpn2I TCCGGA 1 cut(s) 81
Kzo9I GATC 5 cut(s) 62, 172, 177, 285, 339
LmnI GCTCC 3 cut(s) 188, 196, 217
LweI GCATC 1 cut(s) 309
MaeI CTAG 1 cut(s) 224
MaeII ACGT 2 cut(s) 15, 278
MaeIII GTNAC 2 cut(s) 145, 200
MalI GATC 5 cut(s) 64, 174, 179, 287, 341
MbiI CCGCTC 1 cut(s) 44
MboI GATC 5 cut(s) 62, 172, 177, 285, 339
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 1 cut(s) 193
MluI ACGCGT 1 cut(s) 112
MnlI CCTC 9 cut(s) 87, 112, 115, 129, 280, 315, 318, 432, 444
Mph1103I ATGCAT 1 cut(s) 91
MroI TCCGGA 1 cut(s) 81
MseI TTAA 1 cut(s) 426
MspI CCGG 5 cut(s) 82, 165, 215, 409, 430
MspR9I CCNGG 3 cut(s) 71, 353, 410
Mva1269I GAATGC 1 cut(s) 91
MvaI CCWGG 2 cut(s) 71, 353
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 1 cut(s) 218
NciI CCSGG 1 cut(s) 410
NcoI CCATGG 1 cut(s) 194
NdeII GATC 5 cut(s) 62, 172, 177, 285, 339
NlaIII CATG 3 cut(s) 93, 198, 212
NmuCI GTSAC 1 cut(s) 145
NsiI ATGCAT 1 cut(s) 91
NspV TTCGAA 1 cut(s) 403
PctI GAATGC 1 cut(s) 91
PfeI GAWTC 3 cut(s) 58, 254, 405
PflMI CCANNNNNTGG 1 cut(s) 282
PinAI ACCGGT 1 cut(s) 164
Psp124BI GAGCTC 1 cut(s) 193
Psp6I CCWGG 2 cut(s) 69, 351
PspGI CCWGG 2 cut(s) 69, 351
PsuI RGATCY 1 cut(s) 177
RsaI GTAC 1 cut(s) 53
RsaNI GTAC 1 cut(s) 52
SacI GAGCTC 1 cut(s) 193
SaqAI TTAA 1 cut(s) 426
Sau3AI GATC 5 cut(s) 62, 172, 177, 285, 339
ScrFI CCNGG 3 cut(s) 71, 353, 410
SduI GDGCHC 1 cut(s) 193
SetI ASST 8 cut(s) 18, 36, 193, 272, 281, 354, 393, 402
SfaNI GCATC 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 403
SsiI CCGC 2 cut(s) 42, 125
SspMI CTAG 1 cut(s) 224
SstI GAGCTC 1 cut(s) 193
StyD4I CCNGG 3 cut(s) 69, 351, 408
StyI CCWWGG 1 cut(s) 194
TaiI ACGT 2 cut(s) 18, 281
TaqI TCGA 3 cut(s) 61, 117, 403
TatI WGTACW 1 cut(s) 51
TfiI GAWTC 3 cut(s) 58, 254, 405
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TseFI GTSAC 1 cut(s) 145
Tsp45I GTSAC 1 cut(s) 145
Van91I CCANNNNNTGG 1 cut(s) 282
XspI CTAG 1 cut(s) 224
Zsp2I ATGCAT 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.