Rmu_ssc0000064.1_g000022

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000064.1
OG0000187
Physical Location & Seq
Reverse (-)
134640 .. 136817
2178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000064.1_g000022.1.cds

Sequence Viewer

Length: 822 bp
atgtttgctagggaagcgctggtggacgaaagaactccaattgaagcagctagttcagttagagagattattgttttaattgaggagattccagatcgagttgagaagatgattacctcaattgaggaaggcaagggattgcgacaatctgaagttttcatggttgggaaactccttactgctaaggtgttcaaggtccgctccttcctagaccgggctagggtttggaggggtgcgccatggggttttaaccgtggtcatgtggctttggctgagtatgatggagttgtgccaattgagaaggtaccgctggtgaagtcctcttattggatcacgttacaaggtattccacctgctttcacatccgaaagggagatgacgatgattgggtataccttggcggactttcaggagattgataagcaaggaaagaaggttggtaagtatcgtatccggtatgataagttgtttgaaagatgtggggtgtgtggcttggtcacacacgttggcttgccatgctcggggccgccgctgaatgaggaagatgatgatgcggagcgtacagtgttgggcacgtcggcggccatgcaggccggcgtcatgcaggttggggttggggacctgggtacggggtcaggctctgaaggttgcagccatgatgctgctttcactcctatggtttcattcaatgggcatagcttgatattcaaaactcaactcccaagaatgatcccggagcatcttttgtgtaaccctacttacttggtgcagaaaccacgctgtaaggaaagcattagagagattggagattctgacaggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

273

Amino Acids

30.28

Weight (kDa)

6.03

Isoelectric Point (pI)

26.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000187)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20170 FvH4_4g06831
rosa_chinensis RchiOBHm_Chr1g0321991 RchiOBHm_Chr2g0161811
rosa_laevigata RLG00000015375 RLG00000018377
rosa_multiflora Rmu_co8011754.1_g000001 Rmu_co8019986.1_g000001 Rmu_co8067766.1_g000001 Rmu_co8156128.1_g000001 Rmu_co8219154.1_g000003 Rmu_co8297235.1_g000001 Rmu_co8345223.1_g000001 Rmu_co8364775.1_g000001 Rmu_co8465075.1_g000001 Rmu_sc0000007.1_g000002 Rmu_sc0000054.1_g000015 Rmu_sc0000054.1_g000023 Rmu_sc0000145.1_g000010 Rmu_sc0000166.1_g000006 Rmu_sc0000218.1_g000012 Rmu_sc0000339.1_g000008 Rmu_sc0000342.1_g000015 Rmu_sc0000464.1_g000008 Rmu_sc0000620.1_g000003 Rmu_sc0000638.1_g000017 Rmu_sc0000890.1_g000007 Rmu_sc0000894.1_g000024 Rmu_sc0000909.1_g000010 Rmu_sc0000928.1_g000020 Rmu_sc0000977.1_g000007 Rmu_sc0001022.1_g000003 Rmu_sc0001035.1_g000037 Rmu_sc0001060.1_g000008 Rmu_sc0001168.1_g000005 Rmu_sc0001173.1_g000022 Rmu_sc0001179.1_g000016 Rmu_sc0001408.1_g000003 Rmu_sc0001455.1_g000005 Rmu_sc0001484.1_g000008 Rmu_sc0001585.1_g000010 Rmu_sc0001702.1_g000030 Rmu_sc0001755.1_g000063 Rmu_sc0001903.1_g000009 Rmu_sc0002099.1_g000001 Rmu_sc0002142.1_g000012 Rmu_sc0002158.1_g000014 Rmu_sc0002307.1_g000009 Rmu_sc0002358.1_g000013 Rmu_sc0002421.1_g000002 Rmu_sc0002426.1_g000003 Rmu_sc0002438.1_g000001 Rmu_sc0002461.1_g000001 Rmu_sc0002508.1_g000003 Rmu_sc0002532.1_g000056 Rmu_sc0002583.1_g000002 Rmu_sc0002927.1_g000033 Rmu_sc0003033.1_g000040 Rmu_sc0003271.1_g000006 Rmu_sc0003381.1_g000006 Rmu_sc0003507.1_g000007 Rmu_sc0003578.1_g000003 Rmu_sc0003693.1_g000004 Rmu_sc0003693.1_g000005 Rmu_sc0003704.1_g000003 Rmu_sc0003840.1_g000002 Rmu_sc0004084.1_g000026 Rmu_sc0004193.1_g000007 Rmu_sc0004234.1_g000033 Rmu_sc0004235.1_g000025 Rmu_sc0004435.1_g000006 Rmu_sc0004727.1_g000003 Rmu_sc0004741.1_g000001 Rmu_sc0004926.1_g000004 Rmu_sc0005365.1_g000001 Rmu_sc0005392.1_g000007 Rmu_sc0005415.1_g000001 Rmu_sc0005539.1_g000007 Rmu_sc0005708.1_g000017 Rmu_sc0006317.1_g000007 Rmu_sc0006453.1_g000054 Rmu_sc0006536.1_g000004 Rmu_sc0006740.1_g000022 Rmu_sc0006763.1_g000012 Rmu_sc0007115.1_g000002 Rmu_sc0007115.1_g000010 Rmu_sc0007129.1_g000017 Rmu_sc0007131.1_g000010 Rmu_sc0008373.1_g000008 Rmu_sc0009005.1_g000008 Rmu_sc0009252.1_g000007 Rmu_sc0009599.1_g000022 Rmu_sc0009785.1_g000027 Rmu_sc0009891.1_g000003 Rmu_sc0010099.1_g000010 Rmu_sc0010266.1_g000008 Rmu_sc0010334.1_g000003 Rmu_sc0010381.1_g000001 Rmu_sc0010496.1_g000003 Rmu_sc0010496.1_g000004 Rmu_sc0010546.1_g000003 Rmu_sc0011482.1_g000006 Rmu_sc0011875.1_g000010 Rmu_sc0011917.1_g000006 Rmu_sc0012293.1_g000004 Rmu_sc0012847.1_g000003 Rmu_sc0013773.1_g000001 Rmu_sc0016060.1_g000003 Rmu_sc0016170.1_g000013 Rmu_sc0016393.1_g000002 Rmu_sc0017589.1_g000001 Rmu_sc0017786.1_g000005 Rmu_sc0018326.1_g000002 Rmu_sc0020760.1_g000001 Rmu_sc0021262.1_g000003 Rmu_sc0023851.1_g000002 Rmu_sc0024044.1_g000001 Rmu_sc0026820.1_g000001 Rmu_sc0032306.1_g000001 Rmu_sc0035666.1_g000001 Rmu_sc0036881.1_g000001 Rmu_sc0037030.1_g000001 Rmu_sc0037158.1_g000001 Rmu_ssc0000016.1_g000002 Rmu_ssc0000044.1_g000012 Rmu_ssc0000044.1_g000014 Rmu_ssc0000050.1_g000064 Rmu_ssc0000064.1_g000022 Rmu_ssc0000154.1_g000037 Rmu_ssc0000178.1_g000013 Rmu_ssc0000217.1_g000022 Rmu_ssc0000392.1_g000004
rosa_roxburghii Rroxscaffold_3G00242000 Rroxscaffold_3G00242530 Rroxscaffold_6G00406010
rosa_rugosa Rorug01G0062000 Rorug02G0224000 Rorug02G0262900 Rorug02G0263000 Rorug02G0358900 Rorug02G0360500 Rorug02G0378500.1 Rorug02G0378600 Rorug02G0394700 Rorug03G0122900 Rorug03G0254600 Rorug03G0254700 Rorug03G0254800 Rorug04G0042200.1 Rorug04G0378100 Rorug05G0053400 Rorug05G0238600 Rorug05G0364500 Rorug05G0562000 Rorug06G0089300 Rorug06G0089400 Rorug06G0145700 Rorug07G0111600 Rorug07G0229500.1 Rorug07G0287600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 361
Acc36I ACCTGC 2 cut(s) 361, 595
Acc65I GGTACC 1 cut(s) 304
AccB1I GGYRCC 1 cut(s) 304
AccBSI CCGCTC 1 cut(s) 201
AccI GTMKAC 1 cut(s) 392
AciI CCGC 7 cut(s) 199, 308, 401, 527, 530, 554, 581
AclWI GGATC 2 cut(s) 338, 724
AcoI YGGCCR 1 cut(s) 582
AcuI CTGAAG 2 cut(s) 171, 663
AcyI GRCGYC 1 cut(s) 597
AfaI GTAC 3 cut(s) 306, 562, 628
AfeI AGCGCT 1 cut(s) 18
AfiI CCNNNNNNNGG 5 cut(s) 214, 220, 327, 521, 628
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 5 cut(s) 44, 193, 473, 688, 709
AjiI CACGTC 1 cut(s) 576
AjnI CCWGG 1 cut(s) 621
AluBI AGCT 2 cut(s) 50, 699
AluI AGCT 2 cut(s) 50, 699
AlwI GGATC 2 cut(s) 338, 724
AlwNI CAGNNNCTG 1 cut(s) 641
Ama87I CYCGRG 1 cut(s) 520
Aor51HI AGCGCT 1 cut(s) 18
AoxI GGCC 3 cut(s) 524, 582, 591
ApeKI GCWGC 3 cut(s) 47, 651, 662
Asp718I GGTACC 1 cut(s) 304
AspLEI GCGC 2 cut(s) 19, 238
AspS9I GGNCC 3 cut(s) 196, 524, 619
AsuC2I CCSGG 2 cut(s) 215, 734
AsuHPI GGTGA 1 cut(s) 325
AvaI CYCGRG 1 cut(s) 520
AvaII GGWCC 2 cut(s) 196, 619
BaeGI GKGCMC 1 cut(s) 575
BanI GGYRCC 1 cut(s) 304
BbvI GCAGC 3 cut(s) 59, 649, 663
BccI CCATC 1 cut(s) 275
BciT130I CCWGG 1 cut(s) 623
BciVI GTATCC 1 cut(s) 461
BcnI CCSGG 2 cut(s) 215, 734
BfaI CTAG 4 cut(s) 9, 51, 209, 219
BfoI RGCGCY 1 cut(s) 20
BfuAI ACCTGC 2 cut(s) 361, 595
BfuI GTATCC 1 cut(s) 461
BglI GCCNNNNNGGC 1 cut(s) 590
BisI GCNGC 6 cut(s) 48, 527, 530, 582, 652, 663
BlsI GCNGC 6 cut(s) 49, 528, 531, 583, 653, 664
Bme1390I CCNGG 3 cut(s) 215, 623, 734
Bme18I GGWCC 2 cut(s) 196, 619
BmeT110I CYCGRG 1 cut(s) 520
BmgBI CACGTC 1 cut(s) 576
BmgT120I GGNCC 3 cut(s) 196, 524, 619
BmiI GGNNCC 3 cut(s) 306, 525, 620
BmrFI CCNGG 3 cut(s) 215, 623, 734
BmsI GCATC 3 cut(s) 541, 649, 748
Bpu10I CCTNAGC 1 cut(s) 183
BpuMI CCSGG 2 cut(s) 215, 734
BsaHI GRCGYC 1 cut(s) 597
BsaJI CCNNGG 4 cut(s) 239, 253, 396, 622
BsaWI WCCGGW 1 cut(s) 453
Bsc4I CCNNNNNNNGG 5 cut(s) 214, 220, 327, 521, 628
Bse118I RCCGGY 1 cut(s) 593
BseBI CCWGG 1 cut(s) 623
BseDI CCNNGG 4 cut(s) 239, 253, 396, 622
BseGI GGATG 1 cut(s) 362
BseLI CCNNNNNNNGG 5 cut(s) 214, 220, 327, 521, 628
BseMII CTCAG 1 cut(s) 264
BseRI GAGGAG 1 cut(s) 98
BseSI GKGCMC 1 cut(s) 575
BseXI GCAGC 3 cut(s) 59, 649, 663
BsgI GTGCAG 1 cut(s) 788
BshFI GGCC 3 cut(s) 526, 584, 593
BshNI GGYRCC 1 cut(s) 304
BsiHKCI CYCGRG 1 cut(s) 520
BsiSI CCGG 4 cut(s) 214, 454, 594, 734
BslFI GGGAC 1 cut(s) 632
BslI CCNNNNNNNGG 5 cut(s) 214, 220, 327, 521, 628
BsmFI GGGAC 1 cut(s) 632
BsnI GGCC 3 cut(s) 526, 584, 593
BsoBI CYCGRG 1 cut(s) 520
Bsp1286I GDGCHC 1 cut(s) 575
Bsp143I GATC 3 cut(s) 94, 330, 729
Bsp19I CCATGG 1 cut(s) 239
BspACI CCGC 7 cut(s) 199, 308, 401, 527, 530, 554, 581
BspANI GGCC 3 cut(s) 526, 584, 593
BspCNI CTCAG 1 cut(s) 265
BspLI GGNNCC 3 cut(s) 306, 525, 620
BspMI ACCTGC 2 cut(s) 361, 595
BspPI GGATC 2 cut(s) 338, 724
BspT107I GGYRCC 1 cut(s) 304
BsrBI CCGCTC 1 cut(s) 201
BsrFI RCCGGY 1 cut(s) 593
BssAI RCCGGY 1 cut(s) 593
BssECI CCNNGG 4 cut(s) 239, 253, 396, 622
BssMI GATC 3 cut(s) 94, 330, 729
BssNAI GTATAC 1 cut(s) 393
BssNI GRCGYC 1 cut(s) 597
BssT1I CCWWGG 2 cut(s) 239, 396
Bst1107I GTATAC 1 cut(s) 393
Bst2UI CCWGG 1 cut(s) 623
Bst4CI ACNGT 2 cut(s) 254, 565
BstACI GRCGYC 1 cut(s) 597
BstC8I GCNNGC 3 cut(s) 512, 591, 595
BstDEI CTNAG 2 cut(s) 183, 273
BstDSI CCRYGG 2 cut(s) 239, 253
BstF5I GGATG 1 cut(s) 362
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 2 cut(s) 19, 238
BstKTI GATC 3 cut(s) 97, 333, 732
BstMBI GATC 3 cut(s) 94, 330, 729
BstMWI GCNNNNNNNGC 3 cut(s) 14, 516, 590
BstNI CCWGG 1 cut(s) 623
BstSCI CCNGG 3 cut(s) 213, 621, 732
BstSLI GKGCMC 1 cut(s) 575
BstV1I GCAGC 3 cut(s) 59, 649, 663
BstZ17I GTATAC 1 cut(s) 393
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 3 cut(s) 526, 584, 593
BtgI CCRYGG 2 cut(s) 239, 253
BtrI CACGTC 1 cut(s) 576
BtsCI GGATG 1 cut(s) 362
BtsIMutI CAGTG 1 cut(s) 570
BveI ACCTGC 2 cut(s) 361, 595
Cac8I GCNNGC 3 cut(s) 512, 591, 595
CaiI CAGNNNCTG 1 cut(s) 641
CfoI GCGC 2 cut(s) 19, 238
Cfr10I RCCGGY 1 cut(s) 593
Cfr13I GGNCC 3 cut(s) 196, 524, 619
CseI GACGC 1 cut(s) 586
Csp6I GTAC 3 cut(s) 305, 561, 627
CviAII CATG 7 cut(s) 160, 240, 260, 516, 586, 601, 656
CviQI GTAC 3 cut(s) 305, 561, 627
DdeI CTNAG 2 cut(s) 183, 273
DpnI GATC 3 cut(s) 96, 332, 731
DpnII GATC 3 cut(s) 94, 330, 729
EaeI YGGCCR 1 cut(s) 582
EciI GGCGGA 1 cut(s) 416
Eco130I CCWWGG 2 cut(s) 239, 396
Eco47I GGWCC 2 cut(s) 196, 619
Eco47III AGCGCT 1 cut(s) 18
Eco57I CTGAAG 2 cut(s) 171, 663
Eco88I CYCGRG 1 cut(s) 520
EcoO109I RGGNCCY 1 cut(s) 619
EcoRII CCWGG 1 cut(s) 621
EcoT14I CCWWGG 2 cut(s) 239, 396
ErhI CCWWGG 2 cut(s) 239, 396
FaeI CATG 7 cut(s) 163, 243, 263, 519, 589, 604, 659
FaqI GGGAC 1 cut(s) 632
FatI CATG 7 cut(s) 159, 239, 259, 515, 585, 600, 655
FblI GTMKAC 1 cut(s) 392
Fnu4HI GCNGC 6 cut(s) 48, 527, 530, 582, 652, 663
FokI GGATG 1 cut(s) 349
Fsp4HI GCNGC 6 cut(s) 48, 527, 530, 582, 652, 663
FspBI CTAG 4 cut(s) 9, 51, 209, 219
GlaI GCGC 2 cut(s) 18, 237
GluI GCNGC 6 cut(s) 48, 527, 530, 582, 652, 663
HaeII RGCGCY 1 cut(s) 20
HaeIII GGCC 3 cut(s) 526, 584, 593
HapII CCGG 4 cut(s) 214, 454, 594, 734
HgaI GACGC 1 cut(s) 586
HhaI GCGC 2 cut(s) 19, 238
Hin1I GRCGYC 1 cut(s) 597
Hin1II CATG 7 cut(s) 163, 243, 263, 519, 589, 604, 659
Hin6I GCGC 2 cut(s) 17, 236
HinP1I GCGC 2 cut(s) 17, 236
HinfI GANTC 2 cut(s) 88, 809
HpaII CCGG 4 cut(s) 214, 454, 594, 734
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 2 cut(s) 25, 393
Hpy188I TCNGA 4 cut(s) 151, 367, 643, 814
Hpy188III TCNNGA 2 cut(s) 92, 410
Hpy8I GTNNAC 2 cut(s) 25, 393
Hpy99I CGWCG 1 cut(s) 580
HpyAV CCTTC 5 cut(s) 122, 214, 295, 427, 638
HpyCH4III ACNGT 2 cut(s) 254, 565
HpyCH4IV ACGT 3 cut(s) 335, 504, 575
HpyCH4V TGCA 4 cut(s) 589, 604, 651, 769
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 516, 590
HpyF3I CTNAG 2 cut(s) 183, 273
HpySE526I ACGT 3 cut(s) 335, 504, 575
Hsp92I GRCGYC 1 cut(s) 597
Hsp92II CATG 7 cut(s) 163, 243, 263, 519, 589, 604, 659
HspAI GCGC 2 cut(s) 17, 236
KpnI GGTACC 1 cut(s) 308
KroI GCCGGC 1 cut(s) 593
KroNI GCCGGC 1 cut(s) 595
Kzo9I GATC 3 cut(s) 94, 330, 729
LmnI GCTCC 3 cut(s) 206, 556, 736
Lsp1109I GCAGC 3 cut(s) 59, 649, 663
LweI GCATC 3 cut(s) 541, 649, 748
MaeI CTAG 4 cut(s) 9, 51, 209, 219
MaeII ACGT 3 cut(s) 335, 504, 575
MaeIII GTNAC 3 cut(s) 336, 496, 749
MalI GATC 3 cut(s) 96, 332, 731
MbiI CCGCTC 1 cut(s) 201
MboI GATC 3 cut(s) 94, 330, 729
MboII GAAGA 2 cut(s) 118, 554
MfeI CAATTG 3 cut(s) 39, 120, 294
MhlI GDGCHC 1 cut(s) 575
MluCI AATT 4 cut(s) 39, 78, 120, 294
MnlI CCTC 6 cut(s) 76, 118, 127, 222, 331, 532
MroNI GCCGGC 1 cut(s) 593
MseI TTAA 2 cut(s) 77, 249
MslI CAYNNNNRTG 1 cut(s) 674
MspA1I CMGCKG 2 cut(s) 310, 532
MspI CCGG 4 cut(s) 214, 454, 594, 734
MspR9I CCNGG 3 cut(s) 215, 623, 734
MunI CAATTG 3 cut(s) 39, 120, 294
MvaI CCWGG 1 cut(s) 623
MwoI GCNNNNNNNGC 3 cut(s) 14, 516, 590
NaeI GCCGGC 1 cut(s) 595
NciI CCSGG 2 cut(s) 215, 734
NcoI CCATGG 1 cut(s) 239
NdeII GATC 3 cut(s) 94, 330, 729
NgoMIV GCCGGC 1 cut(s) 593
NlaIII CATG 7 cut(s) 163, 243, 263, 519, 589, 604, 659
NlaIV GGNNCC 3 cut(s) 306, 525, 620
NmuCI GTSAC 1 cut(s) 496
PaqCI CACCTGC 1 cut(s) 361
PdiI GCCGGC 1 cut(s) 595
PfeI GAWTC 2 cut(s) 88, 809
PfoI TCCNGGA 1 cut(s) 732
PkrI GCNGC 6 cut(s) 49, 528, 531, 583, 653, 664
PpuMI RGGWCCY 1 cut(s) 619
Psp5II RGGWCCY 1 cut(s) 619
Psp6I CCWGG 1 cut(s) 621
PspGI CCWGG 1 cut(s) 621
PspN4I GGNNCC 3 cut(s) 306, 525, 620
PspPI GGNCC 3 cut(s) 196, 524, 619
PspPPI RGGWCCY 1 cut(s) 619
PstNI CAGNNNCTG 1 cut(s) 641
RsaI GTAC 3 cut(s) 306, 562, 628
RsaNI GTAC 3 cut(s) 305, 561, 627
RseI CAYNNNNRTG 1 cut(s) 674
SaqAI TTAA 2 cut(s) 77, 249
SatI GCNGC 6 cut(s) 48, 527, 530, 582, 652, 663
Sau3AI GATC 3 cut(s) 94, 330, 729
Sau96I GGNCC 3 cut(s) 196, 524, 619
ScrFI CCNGG 3 cut(s) 215, 623, 734
SduI GDGCHC 1 cut(s) 575
SfaNI GCATC 3 cut(s) 541, 649, 748
SfiI GGCCNNNNNGGCC 1 cut(s) 590
SinI GGWCC 2 cut(s) 196, 619
SmiMI CAYNNNNRTG 1 cut(s) 674
Sse9I AATT 4 cut(s) 39, 78, 120, 294
SsiI CCGC 7 cut(s) 199, 308, 401, 527, 530, 554, 581
SspMI CTAG 4 cut(s) 9, 51, 209, 219
StyD4I CCNGG 3 cut(s) 213, 621, 732
StyI CCWWGG 2 cut(s) 239, 396
TaaI ACNGT 2 cut(s) 254, 565
TaiI ACGT 3 cut(s) 338, 507, 578
TaqI TCGA 1 cut(s) 97
TasI AATT 4 cut(s) 39, 78, 120, 294
TauI GCSGC 3 cut(s) 529, 532, 584
TfiI GAWTC 2 cut(s) 88, 809
Tru1I TTAA 2 cut(s) 77, 249
Tru9I TTAA 2 cut(s) 77, 249
TscAI CASTG 1 cut(s) 570
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 3 cut(s) 47, 651, 662
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 2 cut(s) 148, 672
TspRI CASTG 1 cut(s) 570
VpaK11BI GGWCC 2 cut(s) 196, 619
XmiI GTMKAC 1 cut(s) 392
XspI CTAG 4 cut(s) 9, 51, 209, 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.